CN103833803A - 超声波提取和树脂纯化多穗柯叶中甜味剂的方法 - Google Patents
超声波提取和树脂纯化多穗柯叶中甜味剂的方法 Download PDFInfo
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- CN103833803A CN103833803A CN201410067676.2A CN201410067676A CN103833803A CN 103833803 A CN103833803 A CN 103833803A CN 201410067676 A CN201410067676 A CN 201410067676A CN 103833803 A CN103833803 A CN 103833803A
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Cited By (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104861012A (zh) * | 2015-04-20 | 2015-08-26 | 湖南农业大学 | 一种分离制备多穗石柯根皮苷单体的方法 |
CN106220693A (zh) * | 2016-07-29 | 2016-12-14 | 广西苷亮健生物科技有限公司 | 一种提取多穗石柯根皮苷的方法 |
CN108541948A (zh) * | 2018-07-16 | 2018-09-18 | 怀化学院 | 一种木姜叶柯含片及其制备方法 |
CN108902972A (zh) * | 2018-07-16 | 2018-11-30 | 怀化学院 | 一种木姜叶柯咀嚼片及其制备方法 |
CN109134557A (zh) * | 2018-07-16 | 2019-01-04 | 华北理工大学 | 从多穗石柯叶片中提取根皮苷的方法 |
CN110041188A (zh) * | 2019-05-22 | 2019-07-23 | 东华理工大学 | 一种自多穗柯叶中同时提取二氢查耳酮和棕色素的方法 |
CN110150623A (zh) * | 2019-05-31 | 2019-08-23 | 广西苷亮健生物科技有限公司 | 一种木姜叶柯甜味颗粒及其制备方法 |
CN111345460A (zh) * | 2018-12-20 | 2020-06-30 | 重庆积玉生物工程有限公司 | 一种多穗石柯天然甜味剂的制作方法 |
CN112225768A (zh) * | 2020-11-18 | 2021-01-15 | 成都农业科技中心 | 从木姜叶柯中提取三叶苷和根皮苷的方法 |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103005091A (zh) * | 2012-11-30 | 2013-04-03 | 李胜华 | 一种多穗柯叶速溶茶粉及其制作工艺 |
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2014
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Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103005091A (zh) * | 2012-11-30 | 2013-04-03 | 李胜华 | 一种多穗柯叶速溶茶粉及其制作工艺 |
Non-Patent Citations (3)
Title |
---|
于丽静: "《多穗柯中二氢查耳酮纯化的研究》", 《现代食品科技》 * |
廖晓峰: "《从多穗柯中提取二氢查耳酮(DHC)类甜味剂的研究》", 《广州食品工业科技》 * |
李胜华: "《大孔树脂纯化多穗柯总黄酮的工艺研究》", 《江苏农业科学》 * |
Cited By (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104861012A (zh) * | 2015-04-20 | 2015-08-26 | 湖南农业大学 | 一种分离制备多穗石柯根皮苷单体的方法 |
CN106220693A (zh) * | 2016-07-29 | 2016-12-14 | 广西苷亮健生物科技有限公司 | 一种提取多穗石柯根皮苷的方法 |
CN108541948A (zh) * | 2018-07-16 | 2018-09-18 | 怀化学院 | 一种木姜叶柯含片及其制备方法 |
CN108902972A (zh) * | 2018-07-16 | 2018-11-30 | 怀化学院 | 一种木姜叶柯咀嚼片及其制备方法 |
CN109134557A (zh) * | 2018-07-16 | 2019-01-04 | 华北理工大学 | 从多穗石柯叶片中提取根皮苷的方法 |
CN109134557B (zh) * | 2018-07-16 | 2020-07-28 | 华北理工大学 | 从多穗石柯叶片中提取根皮苷的方法 |
CN111345460A (zh) * | 2018-12-20 | 2020-06-30 | 重庆积玉生物工程有限公司 | 一种多穗石柯天然甜味剂的制作方法 |
CN110041188A (zh) * | 2019-05-22 | 2019-07-23 | 东华理工大学 | 一种自多穗柯叶中同时提取二氢查耳酮和棕色素的方法 |
CN110150623A (zh) * | 2019-05-31 | 2019-08-23 | 广西苷亮健生物科技有限公司 | 一种木姜叶柯甜味颗粒及其制备方法 |
CN112225768A (zh) * | 2020-11-18 | 2021-01-15 | 成都农业科技中心 | 从木姜叶柯中提取三叶苷和根皮苷的方法 |
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