CN103830245A - Oral testosterone ester formulation and application of same in preparation of drug used for treating testosterone deficiency - Google Patents
Oral testosterone ester formulation and application of same in preparation of drug used for treating testosterone deficiency Download PDFInfo
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- CN103830245A CN103830245A CN201410005248.7A CN201410005248A CN103830245A CN 103830245 A CN103830245 A CN 103830245A CN 201410005248 A CN201410005248 A CN 201410005248A CN 103830245 A CN103830245 A CN 103830245A
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Abstract
The invention provides an oral pharmaceutical composition. The oral pharmaceutical composition comprises testosterone undecanoate dissolving in a carrier; the carrier comprises at least one lipophilic surfactant and at least one hydrophilic surfactant, wherein a ratio (w/w) of total lipophilic surfactants to total hydrophilic surfactants is in a range of 6: 1 to 3.5: 1; after oral administration of the composition once or twice each day, a stable state average serum testosterone concentration in a range from 300 to about 1100 ng/dL is provided, and dissolved testosterone undecanoate contains 18 to 22 wt% of the composition. The invention further provides a medicinal preparation of testosterone undecanoate. The invention also provides application of the composition in preparation of a drug used for treating testosterone deficiency or symptoms thereof. The oral pharmaceutical composition provided by the invention can offer an optimal serum testosterone level, and thus, the pharmaceutical composition can effectively treat males with hypogonadism in clinical practice in a prolonged time quantum.
Description
The application is to be on 04 12nd, 2010 the applying date, international application no PCT/US2010/030788, national applications number is 201080066142.8, and denomination of invention is the divisional application of the application of " method of oral testosterone ester formulation and the treatment testosterone deficiency disease that comprises it ".
Technical field
The present invention relates generally to the oral formulations of the testosterone ester that is used for the treatment of testosterone deficiency disease.More specifically, the present invention relates to comprise the pharmaceutical composition of the testosterone undecanoate (TU) with the absorption that strengthens and extend and pharmacokinetics.
background of invention
Testosterone (T) is the main androgen of one that produces in interstitial cell and is responsible for male genitals and the normal growth of secondary sex characteristics (for example, have a low and deep voice, muscle development, facial hair etc.), grows and maintain.In the manhood, testosterone be testis with and accessory structure (prostate and seminal vesicle) normal function, happiness and maintain libido, erectile ability is necessary.
The T hyposecretion of testosterone deficiency disease-taking low serum T concentration as feature-can cause andriatria disease (for example, hypogonadism).The symptom relevant to male gonad hypofunction comprises sexual impotence and hyposexuality, fatigue and there is no that energy, depressed, secondary sex characteristics are degenerated, muscle weight reduces and fat weight increases.And male gonad hypofunction is a risk factor of osteoporosis, metabolic syndrome, type ii diabetes and cardiovascular disease.
The multiple testosterone alternative medicine of commercially available acquisition is treated male gonad hypofunction.Pharmaceutical preparation with intramuscular injection, implant, alkylation T(for example comprises, methyltestosterone) testosterone and the testosterone derivative of oral tablet, topical gel or topical plaster form.But current all T therapies fail suitably to provide that a kind of T's of sending is simple and easy
andclinical effective method.For example, intramuscular injection make people's pain and to dosage between the remarkable fluctuation of serum T level relevant; T paster is general is correlated with and conventionally causes essence skin irritation with the T level (, invalid clinically) below normal value; And T gel is transferred to women and child is relevant to T from user insecurely.Equally, unique " approval " oral T therapy, methyltestosterone is relevant to remarkable hepatotoxic appearance.Therefore, in the course of time, it is good and therefore cause not satisfied low T male treatment that the method for the treatment of at present testosterone deficiency disease is not suffered from compliance.
Testosterone with and the bioavailability of ester not good-because of headed by cross intestinal and liver metabolism significantly-or invalid-because health can not discharge testosterone from its testosterone prodrug.For example, the testosterone ester that testosterone and side chain lengths are less than 10 carbon is mainly to absorb by Portal circulation, causes a large amount of (if not all) first pass metabolism.Long carbochain (that is, 14 or more carbon) fatty acid ester can be by intestinal Lymphatic, but fatty acid chain length is longer, and speed and degree that esterase hydrolyzed ester discharges testosterone are lower, therefore pharmacologically active not good (, invalid clinically).
Except the selection of testosterone ester, the preparation of testosterone ester has proposed unique challenges.Gastrointestinal tract environment is obviously aqueous in essence, and this requires medicine must dissolve for absorption.But, testosterone (especially its ester) utmost point is insoluble in water and aqueous medium, even and T or T ester are dissolved in preparation at first, preparation must be able to make medicine maintain solvable or discrete form, and do not precipitate or separately from solution, separate out in vivo (although this character can be tested in vitro, for example,, by preparation content is mixed in simulated intestinal fluid).And oral T preparation must discharge T or T ester effectively according to required release profiles.Therefore, effective preparation of T or T ester must make good solubility and best release and meet balance between target blood plasma or serum-concentration curve.
Because these and other reason, FDA (Food and Drug Adminstration) (FDA) does not ratify the oral formulations of testosterone or testosterone ester so far yet.In fact unique oral testosterone product of being ratified by FDA is that (wherein, the C-17 position covalent bond of methyl and testosterone " core " is to suppress hepatic metabolism for methyltestosterone, so far; Simultaneously note, methyltestosterone is not the prodrug of testosterone) and this approval occur in decades ago.Unfortunately, the use of methyltestosterone and hepatotoxic incidence rate significant correlation, therefore seldom open this medicine and treat low testosterone male.
As mentioned above, the fatty acid ester of testosterone provides the potential testosterone of another kind of form to send (, with " prodrug " form) to health.Once absorption, testosterone can discharge by the effect of non-specific tissue and blood plasma esterase from its ester.And as determined by its octanol (log P) value, by increasing the relative hydrophobicity of testosterone part and the lipophile of gained molecule, this prodrug can be absorbed by intestinal lymph at least in part, thereby reduces the first pass metabolism of liver.Generally speaking, log P value at least 5 and oil-soluble be that the lipophilicity compound of at least 50mg/mL is mainly to carry by lymphsystem.
Although have prospect, testosterone prodrug, comprises testosterone ester, does not prepare in the mode of the effective and lasting level of serum testosterone (, average serum T concentration is within the scope of about 300-1100ng/dL) that reaches gonad function normal level.In fact, the Orally administered pharmaceutical preparation of testosterone prodrug (comprising testosterone ester), is ratified by FDA not yet.
Therefore, still need a kind of oral formulations of testosterone ester, it provides best level of serum testosterone, its hypoglandular male of therapeutic (, those male of serum T concentration≤300ng/dL) effectively clinically within the time period extending.
Brief summary of the invention
In one embodiment of the invention, a kind of combination of oral medication is provided, it comprises the testosterone undecanoate being dissolved in carrier, described carrier comprises at least one lipophilic surfactant and at least one hydrophilic surfactant active with total lipophilic surfactant and the ratio (w/w) of total hydrophilic surfactant active in the scope of about 6:1 to 3.5:1, described compositions once a day or twice of every day Orally administered after, the testosterone concentration of the stable state average serum within the scope of approximately 300 to about 1100ng/dL is provided.In the time using together with meals, pharmaceutical composition provides and is no more than 2500ng/dL, is preferably no more than 1800ng/dL, and is most preferably no more than the C of 1500ng/dL
max.
According to a preferred embodiment, at least one hydrophilic surfactant active comprises Cremophor RH40(polyoxyethylene glycerol trihydroxy stearate); At least one lipophilic surfactant comprises oleic acid.Pharmaceutical composition of the present invention can comprise the testosterone undecanoate of the dissolving of 18 to 22 % by weight, and can not basically contain monohydric alcohol (for example ethanol).
In another embodiment of the present invention, a kind of dosage form of testosterone undecanoate is provided, it comprises the testosterone undecanoate being dissolved in carrier, described carrier comprises at least one lipophilic surfactant and at least one hydrophilic surfactant active, by described dosage form once a day or every day administered twice after suffering from the experimenter of hypogonadism or its symptom, stable state average serum within the scope of approximately 300 to about 1100ng/dL testosterone concentration is provided, avoids exceeding the C of 2500ng/dL simultaneously
maxthe appearance of value, more preferably, avoids exceeding the C of 1800ng/dL
maxthe appearance of value, most preferably, avoids exceeding the C of 1500ng/dL
maxthe appearance of value.
In another embodiment of the present invention, a kind of pharmaceutical composition is provided, it comprises the testosterone undecanoate being dissolved in carrier, described carrier comprises at least one lipophilic surfactant and at least one hydrophilic surfactant active, described compositions with low 20 % by weight that reach of fat content to as high as after Orally administered together with the meals of 50 % by weight, providing relatively with Orally administered rear observed value together with the meals of fat content approximately 30 % by weight is inapparent average serum testosterone concentration statistically.
In an embodiment more of the present invention, a kind of pharmaceutical composition is provided, it comprises the testosterone undecanoate being dissolved in carrier, described carrier comprises at least one lipophilic surfactant and at least one hydrophilic surfactant active with total lipophilic surfactant and the ratio (w/w) of total hydrophilic surfactant active in the scope of about 6:1 to 3.5:1, described compositions once a day or every twice-daily Orally administered after, the quick phase half-life of serum testosterone of approximately 5 hours and the serum testosterone t1/2 of approximately 29 hours are provided.
In an embodiment more of the present invention, a kind of pharmaceutical composition is provided, it comprises the testosterone undecanoate being dissolved in carrier, described carrier comprises at least one lipophilic surfactant and at least one hydrophilic surfactant active with total lipophilic surfactant and the ratio (w/w) of total hydrophilic surfactant active in the scope of about 6:1 to 3.5:1, by described compositions once a day or twice of every day by oral administration to suffering from after the experimenter of testosterone deficiency disease or its symptom, the average serum testosterone concentration of the 30th day at therapeutic scheme every day is provided, it is substantially identical with the 7th day observed value.According to the present invention, every day, the average serum testosterone concentration obtaining for the 30th day of therapeutic scheme was substantially also identical with the observed value of the 60th day.
In another embodiment of the present invention, a kind of method for the treatment of testosterone deficiency disease or its symptom is provided, it comprises the pharmaceutical composition of the Orally administered effective dose of experimenter to suffering from testosterone deficiency disease or its symptom, described pharmaceutical composition comprises the testosterone undecanoate being dissolved in carrier, described carrier comprises at least one lipophilic surfactant and at least one hydrophilic surfactant active with total lipophilic surfactant and total hydrophilic surfactant active's ratio (w/w) at about 6:1 to 3.5:1, so that the testosterone concentration of the stable state average serum within the scope of approximately 300 to about 1100ng/dL to be provided.Described compositions can be once a day or every day administered twice, and can obtain approximately 900 with 1100ng/dL within the scope of C
maxvalue.
According to this method, described compositions can be used together with comprising the meals of at least 20 % by weight fat.Described method does not cause the diurnal variation of testosterone pharmacokinetics, average serum T substantially
maxbe worth within the scope of Orally administered latter approximately 3 to 7 hours, and after repeat administration, substantially do not observe the reaction of stable state serum testosterone and significantly decline.
In a preferred embodiment of the present invention, a kind of pharmaceutical composition is provided, it comprises:
(a) testosterone undecanoate of the dissolving of 15-25 % by weight;
(b) at least one hydrophilic surfactant active of 12-18 % by weight;
(c) at least one lipophilic surfactant of 50-65 % by weight;
(d) borage oil of 10-15 % by weight and the mixture of Oleum menthae,
Described compositions can not contain monohydric alcohol, particularly ethanol conventionally, and to after having the experimenter who needs Orally administered, obtains the serum testosterone half-life (T in the scope of approximately 10 hours to approximately 18 hours
1/2).Cremophor RH40 is that a kind of preferred hydrophilic surfactant active and preferred lipophilic surfactant are oleic acid.Borage oil and Oleum menthae are all considered to lipophilic surfactant.
In an especially preferred embodiment, described compositions comprises:
(a) testosterone undecanoate of the dissolving of 18-22 % by weight;
(b) at least one hydrophilic surfactant active of 15-17 % by weight;
(c) at least one lipophilic surfactant of 50-55 % by weight; And
(d) borage oil of 10-15 % by weight and the mixture of Oleum menthae.
The ratio of borage oil and Oleum menthae can be in 8:1 to 3:1 scope, preferably, and 6:1 to 5:1, more preferably, 5:1 to 4:1.Except Cremophor RH40, SolutolHS-15, Tween80 and TPGS are also preferred hydrophilic surfactant actives; And except oleic acid, glyceryl monooleate, lauric acid propylene glycol ester and Capmul MCM are also preferred lipophilic surfactants.Also expected two or more lipophilic surfactants and two or more hydrophilic surfactant actives' combination.
In another embodiment of the present invention, a kind of method for the treatment of testosterone deficiency disease is provided, described method comprises the pharmaceutical composition to the Orally administered effective dose of hypogonadism experimenter, described pharmaceutical composition comprises:
(a) testosterone undecanoate of the dissolving of 15-25 % by weight;
(b) one or more hydrophilic surfactant actives of 12-18 % by weight;
(c) one or more lipophilic surfactants of 50-65 % by weight;
(d) borage oil of 10-15 % by weight and the mixture of Oleum menthae,
And containing ethanol, described pharmaceutical composition once a day or twice of every day Orally administered obtain experimenter approximately 300 to about 1100ng/dL within the scope of stable state average serum testosterone concentration, C
ave.Described compositions is optionally used together with approximately 15 % by weight and approximately 25 % by weight or higher meals with fat content.According to this method, experimenter can reach any one or all in following pharmacokinetic parameter:
(a) experimenter's serum testosterone C
max900 to 1100ng/dL;
(b) essentially no testosterone pharmacokinetics diurnal variation;
(c) use serum T after compositions
maxit is 3 to 7 hours; And
(d) after repetitive administration, substantially not observing the reaction of stable state serum testosterone declines.
In this respect, explaining before at least one embodiment of the present invention in detail, should understand the present invention and its application is not limited to structural details and be limited to the component configuration illustrating in mentioned in following description or accompanying drawing.The present invention can carry out except embodiment describing and can implementing in many ways and carry out.Equally, should understand the phrase and the term that in literary composition and in summary, use is not to be considered as restriction in order to set forth.
Therefore, those skilled in the art can by understand the disclosure based on concept can be easily as the basis of design other parts, method and system for carrying out multiple object of the present invention.For example, embodiments more of the present invention can combine TU and other active medicines (comprising other hormones) in oral delivery system, described oral delivery components of system as directed ground prevention or the alleviation symptom relevant to testosterone deficiency disease.Therefore, importantly, think that claim comprises the equivalent structure that does not depart from the scope of the invention and spirit.
accompanying drawing summary
Fig. 1 provide once a day or 24 hours of twice oral administration every day TU preparation of the present invention in serum T level.
Fig. 2 illustrates that using preparation contrast of the present invention comprises the male of hypogonadism serum T response in time after the oral TU preparation of the tradition of TU (Restandol) in oleic acid.
Fig. 3 provides before Orally administered TU preparation of the present invention, the T of the experimenter's of the meals of the different fat contents of taking food (representing with percentage by weight) serum T level
maxvalue.
Fig. 4 provides before Orally administered TU preparation of the present invention, the C of the experimenter's of the meals of the different fat contents of taking food (representing with percentage by weight) serum T level
maxvalue.
Fig. 5 provides before Orally administered TU preparation of the present invention, area under curve (AUC) value of the experimenter's of the meals of the different fat contents of taking food (representing with percentage by weight) serum T level.
detailed Description Of The Invention
The invention provides the combination of oral medication of a kind of TU of comprising, said composition is when when being no more than administered twice in the male of hypogonadism every day, provide in this male and to expect " normally " or gonad function normal range (, about 300-1100ng/dL) interior testosterone average steady state serum levels (concentration), avoid being thought by FDA (Food and Drug Adminstration) simultaneously unexpected, or even unacceptable high C
maxvalue.For example, the statement of FDA approval guide is less than 85% treatment experimenter and can has 1500ng/dL or higher C
maxvalue, and nobody has the C that exceeds 2500ng/dL
maxvalue.Be less than 5% treatment experimenter and there is the C within the scope of 1800-2500ng/dL
maxvalue.And, preparation of the present invention is designed to self-emulsifying drug delivery systems (SEDDS) to form the emulsion (or dispersion) containing TU after mixing with gastrointestinal intestinal juice.
In one embodiment of the invention, adopt preparation of the present invention, combine by the ester of oral delivery testosterone and/or testosterone molecule C17 position individually or with other active component.For example, the preferably combination of Orally active inhibitor of testosterone undecanoate and I type or II type 5α-reductase or the combination of testosterone undecanoate and synthetic progesterone in some embodiments.
Although the undecylate (, TU) by testosterone is described and illustration multiple embodiments of the present invention, according to the instruction of description, can adopt other esters (comprising T) of hydrophobic compound for oral delivery.In fact, those of ordinary skill in the art should be apparent from instruction herein, and drug delivery system of the present invention and the compositions of coming since then can be suitable for other testosterone esters of oral delivery, for example short chain (C
2-C
6), medium chain (C
7-C
13) and long-chain (C
14-C
24) fatty acid ester, the preferably medium chain fatty acid ester of testosterone.
Preparation of the present invention comprises the T-ester being dissolved in mixture, and described mixture comprises one or more lipophilic surfactants and one or more hydrophilic surfactant actives.Lipophilic surfactant as defined herein has and is less than 10 and be preferably less than 5 hydrophilic-lipophilic balance (HLB) (HLB) value.Hydrophilic surfactant active as defined herein has the HLB value that is greater than 10.(HLB is that the hydrophilic of the active amphiphatic molecule of a kind of effects on surface (for example, surfactant) and the experience of hydrophobic group relation are expressed.It changes and comprises nonionic and ionic surfactant approximately 1 to approximately 45 in order to presentation surface activating agent and its value.HLB value is higher, and surfactant water solublity/dispersibility is stronger).
According to an aspect of the present invention, in delivery system, each component (, lipophile and hydrophilic surfactant active) has separately property of solubilizing and partly contributes to active component to dissolve.Substantially contribute to those lipophilic surfactants of medicine dissolution to be defined as " mainly " solvent herein.But, should be appreciated that, dissolubility can be affected by the temperature of solvent/preparation.For example, the preparation of the present invention that comprises approximately 20% testosterone undecanoate is at 30 DEG C or above temperature (comprising within the scope of 30 to approximately 40 DEG C) is lower keeps solvable.
Hydrophilic surfactant active's component is that to reach required dispersibility and the drug release of preparation in gastrointestinal tract required., hydrophilic surfactant active, except as secondary solvent, also needs with it, medicine to be discharged from lipid carrier substrate or in primary solvent.In this regard, high HLB surfactant, for example Cremophor RH40, can meet the demands conventionally.Can adjust to provide optimal drug to discharge to the level of high HLB surfactant (amount), and not undermine the dissolving of active component.
The lipophilic surfactant that is suitable for drug delivery system of the present invention comprises:
Fatty acid (C
6-C
24, preferably, C
10-C
24, more preferably, C
14-C
24), for example, sad, capric acid, undecanoic acid, lauric acid, myristic acid, Palmic acid, stearic acid, oleic acid, linoleic acid plus linolenic acid.Preferably oleic acid.
The list of fatty acid-and/or two-glyceride, for example, Imwitor988(is mono--/bis--caprylin), Imwitor742(is mono--/bis--Xin/caprin), Imwitor308(is mono--caprylin), Imwitor191(is mono--tristerin), Softigen701(is mono--/bis--castor oil acid glyceride), Capmul MCM(is mono--/bis--Xin/caprin), Capmul MCM (L) (liquid form of Capmul MCM), Capmul GMO(is mono--olein), Capmul GDL(GLYCERYL DILAURATE), Maisine(is mono--glyceryl linoleate), Peceol(is mono--olein), Myverol18-92(is from the distillation monoglyceride of sunflower oil) and Myverol18-06(from the distillation monoglyceride of oil with hydrogenated soybean), Precirol ATO5(Palmic acid tristerin) and the semi-synthetic glyceride of Gelucire39/01(, , C
12-18single-, two and Three-glycerol ester).The preferred component of this type of lipophilic surfactant is oleic acid, Palmic acid and stearic partial glyceride and composition thereof.
The list of fatty acid-and/or acetas, succinate, lactate, citrate and/or the tartrate of two-glyceride; for example, Myvacet9-45 (distillation acetylated monoglyceride), Miglyol829 (pungent/capric acid two glycerol succinic acid esters), Myverol SMG (list/bis--succinylated monoglycerides), Imwitor370 (Glyceryl stearate citrate), Imwitor375 (glyceryl monostearate/citrate/lactate) and Crodatem T22 (the diacetyl tartrate of monoglyceride).
The propylene glycol list of fatty acid-and/or two-ester, for example, Lauroglycol (PGML), Mirpyl (propylene glycol list myristinate), Captex200 (propylene glycol dicaprylate/dicaprate), Miglyol840 (propylene glycol dicaprylate/dicaprate) and Neobee M-20 (propylene glycol dicaprylate/dicaprate).
The polyglycerin ester of fatty acid, for example Plurol oleique(polyglycerol acrylate), Caprol ET(polyglycereol fatty acid mixed) and Drewpol10.10.10(polyglycerol acrylate).
The castor oil ethoxylate (HLB<10) with low content ethoxylate, for example Etocas5(5 moles of ethylene oxide is reacted with 1 mole of castor oil) and Sandoxylate5(5 moles of ethylene oxide and the reaction of 1 mole of castor oil).
By making oxirane react the acid and the ester ethoxylate (HLB<10) that form with the glyceride of fatty acid or fatty acid, the glyceride of described fatty acid for example, Crodet04(polyoxyethylene (4) lauric acid), Cithrol2MS(polyoxyethylene (2) stearic acid), Marlosol183(polyoxyethylene (3) stearic acid) and Marlowet G12DO(glyceryl 12EO dioleate).
The sorbitan ester of fatty acid, for example, Span20(Span-20), Crill1(Span-20) and Crill4(sorbitan monooleate).
The product of transesterification reaction (HLB<10) of natural or hydrogenated vegetable oil triglyceride and polyalkylene polyhydric alcohol, for example, Labrafil M1944CS(polyoxyethylene almond oil), Labrafil M2125CS(polyoxyethylene Semen Maydis oil) and Gelucire37/06(polyoxyethylene hydrogenated coconut).Preferably Labrafil M1944CS.
Alcohol ethoxylate (HLB<10), for example, the oily ether of Volpo N3(polyoxyethylene (3)), the oily ether of Brij93(polyoxyethylene (2)), Marlowet LA4(polyoxyethylene (4) Laurel ether).
Pluronics, for example, polyoxyethylene-poly(propylene oxide) copolymer and block copolymer (HLB<10), for example, Synperonic PE L42 (HLB=8) and Synperonic PE L61 (HLB=3).
If needed, can use the mixture of suitable lipophilic surfactant (for example, above listed those), in some instances, find that this mixture is useful.
Can use in the present invention any pharmaceutically acceptable hydrophilic surfactant active (, HLB value is greater than 10).Some limiting examples comprise:
Oleum Ricini or castor oil hydrogenated ethoxylate (HLB>10), for example, Cremophor EL (polyoxyethylene (35) Oleum Ricini), Cremophor RH40 (polyoxyethylene (40) castor oil hydrogenated), Etocas40 (polyoxyethylene (40) Oleum Ricini), Nikkol HCO-60 (polyoxyethylene (60) castor oil hydrogenated), Solutol HS-15 (Polyethylene Glycol 660 hydroxy stearic acid esters), Labrasol (caprylyl caproyl PEG-8 glyceride), alpha-tocopherol-PEG-6000-succinate (TPGS) and ascorbic acid-6 cetylate.Preferably Cremophor RH40.
Polyoxyethylene sorbitol acid anhydride derivative of fatty acid, for example, Tween20(polyoxyethylene (20) monolaurate), Tween80(polyoxyethylene (20) monoleate), Crillet4(polyoxyethylene (20) monoleate) and Montanox40(polyoxyethylene (20) monopalmitate).Preferably Tween80(polysorbate80).
Gelucires, preferably, the mono-and diester of Gelucire50/13(Palmic acid and stearic PEG).(with regard to Gelucires, the fusing point of first digit (, 50) tie substance and the corresponding HLB numerical value of second digit (, 13)).
Fatty acid ethoxylate (HLB>10), for example, Myrj45(polyoxyethylene (8) stearate), Tagat L(polyoxyethylene (30) monolaurate), Marlosol1820(polyoxyethylene (20) stearate) and Marlosol OL15(polyoxyethylene (15) oleate).Preferably Myrj45.
Alcohol ethoxylate (HLB>10), for example, the oily ether of Brij96(polyoxyethylene (10)), the oily ether of Volpo015(polyoxyethylene (15)), the oily ether of Marlowet OA30(polyoxyethylene (30)) and Marlowet LMA20(polyoxyethylene (20) C12 – C14 aliphatic ether).
Polyoxyethylene-poly(propylene oxide) copolymer and block copolymer (HLB>10), can trade name Pluronics or Poloxamers buy, for example Poloxamers188 and 407, is also called Syperonic PE L44(HLB=16) and Syperonic F127(HLB=22).
Anion surfactant, for example, sodium lauryl sulfate, enuatrol and dioctyl sodium sulphosuccinate.
Alkylphenol surfactant (HLB>10), for example, Triton N-101(polyoxyethylene (9-10) nonyl phenol) and Synperonic NP9(polyoxyethylene (9) nonyl phenol).
As described in, in one aspect of the invention, in delivery system every kind of component (, lipophile and hydrophilic surfactant active) have separately solvent properties and partly contribute to active component dissolve.By this way, bound by theory or be limited to this theory, the present invention does not need other solvents, for example, and cosolvent, but these are optionally included in system of the present invention and preparation.
Being suitable for optional cosolvent of the present invention is, for example, water, short chain monocarbon alcohol, dihydroxylic alcohols and polyhydric alcohol, for example ethanol, benzylalcohol, glycerol, propylene glycol, Allyl carbonate, have approximately 200 to approximately 10, Polyethylene Glycol, diethylene glycol monoethyl ether (for example, Transcutol HP) and the combination thereof of 000 mean molecule quantity.Preferably, do not comprise this cosolvent, especially ethanol or other monoethanols.
Other oil that can be incorporated in embodiment of the present invention comprise medium chain (C7 – C13) or long-chain (C
14– C
22) fatty acid and low-molecular-weight be (up to C
6) the complete triglyceride of monobasic, binary or polyhydric alcohol.Therefore, some examples for oils of the present invention comprise: vegetable oil (for example, soybean oil, safflower oil, Semen Maydis oil, olive oil, Oleum Ricini, cotton seed oil, Oleum Arachidis hypogaeae semen, Oleum Helianthi, Oleum Cocois, Petiolus Trachycarpi oil, Oleum Brassicae campestris, Radix Oenotherae erythrosepalae oil, Oleum Vitis viniferae, Semen Tritici aestivi germ oil, Oleum sesami, American Avocado Tree oil, Semen Armeniacae Amarum, borage, Herba Menthae and almond oil) and animal oil (for example, cod-liver oil, shark oil and ermine oil).
In other embodiments of the present invention, method and composition be can biochemical upper (1) TU of adjustment absorb by being incorporated to, (2) TU is metabolized to T and/or (3) T is metabolized to dihydrotestosterone (DHT) component adjusts (, maintaining) serum testosterone availability.For example, in long-chain fatty acid ester, comprise medium and can improve TU absorption.By this way, more TU avoid the hydrolysis in intestinal and enter blood flow.In other words, fatty acid ester can suppress esterase with competing, otherwise esterase is by metabolism TU.The example of other esters or its combination comprises galenical extract or the optimum ester (for example, propyl p-hydroxybenzoate, Caprylyl acetate and ethyl acetate) as food additive.
Other components that capable of regulating TU absorbs comprise that " natural " and the synthetic inhibitor of 5α-reductase, 5α-reductase are the enzymes that a kind of catalysis T of existing in enterocyte and its hetero-organization changes into DHT.The increase that suppresses to increase and to maintain wholly or in part T serum levels after oral administration TU of this conversion, follows simultaneously and reduces serum DHT level.Borage oil, the 5α-reductase inhibitor gamma-Linolenic acid (GLA) that it comprises significant quantity, is an example of " natural " regulator of TU metabolism.Certainly, except in borage oil, independent component that can be using GLA as TU preparation of the present invention and directly adding.(for example in this area, understand the natural inhibitor of multiple 5α-reductase, epigallocatechin gallate (EGCG), it is for the catechuic acid that mainly obtains from green tea with from the Serenoa repens extract of the berry of saw leaf palmetto (Serenoa repens) species), all these can be suitable for the present invention.The limiting examples that is applicable to synthetic 5α-reductase inhibitor of the present invention comprises the compound such as finasteride (finasteride), dutasteride (dutasteride) etc.
Except 5α-reductase inhibitor, the T metabolic poison by other mechanism is contained in the present invention.A kind of this inhibition point can be cytochrome P 450 isozymes CYP3A4, and it is present in also therefore can metabolism testosterone in enterocyte and hepatocyte.Therefore, selected embodiment of the present invention comprises Oleum menthae, and known its contains the component that can suppress CYP3A4 activity.
Other optional members that can be included in the present composition are those compositions that are usually used in oil base drug delivery system, for example, antioxidant, for example, tocopherol, tocopherol acetas, ascorbic acid, butylated hydroxytoluene (BHT), ascorbyl palmitate, fourth oxybenzene methyl ether and propyl gallate; PH stabilizing agent, for example citric acid, tartaric acid, fumaric acid, acetic acid, glycine, arginine, lysine and dipotassium hydrogen phosphate; Thickening agent/suspending agent, for example hydrogenated vegetable oil, Apis cerana Fabricius, silica sol, mannitol, natural gum, cellulose, silicate, bentonite; Flavoring agent, for example Fructus Pruni pseudocerasi, Fructus Citri Limoniae and Fructus Foeniculi spice; Sweeting agent, for example aspartame (aspartame), acesulfame potassium, sucralose, glucide and cyclamate; Deng.
The relative scale that the inventor recognizes one or more lipophilic surfactants and one or more hydrophilic surfactant actives is most important to reaching pharmacokinetics required for the present invention.More specifically, inventor has found total lipophilic surfactant and total hydrophilic surfactant active's a ratio, this ratio not only can be dissolved relatively a large amount of T-ester (for example, being greater than 15%, 18%, 20%, 22% or 25%) and can be provided T-ester to discharge from the best of preparation.Preferably, total oil (for example, oleic acid+borage oil+Oleum menthae, all these are regarded as lipophilic surfactant) with hydrophilic surfactant active's ratio (w/w) in about 6:1 to 1:1,6:1 to 3.1,6:1 to 3.5:1 or 6:1 to 4:1 scope, and more preferably, in about 5:1 to 3:1 scope, and most preferably, in about 4:1 to 3:1 scope.
By weight, preferred following relative concentration (percent is based on total formulation weight amount):
Hydrophilic surfactant active: 10-20%, more preferably, 12-18%, and most preferably, 15-17%.
Lipophilic surfactant: 50-70%, more preferably, 50-65%, and most preferably, 50-55%.
Other oils: 5-15%, more preferably, 7-15%, and most preferably, 10-13%.
Medicine: 10-30%, more preferably, 15-25%, and most preferably, 18-22%.
Preparation of the present invention has self emulsifying, forms thin matter emulsifying agent in vivo after use medium or intestinal juice dilution.In other words, preparation can have high surfactant and lipid content, and it is designed for and after mixing with water-bearing media, forms ideal dispersion body.The qualitative description of the self emulsifying characteristic of preparation of the present invention can be observed during its dissolution in vitro.On the other hand, can utilize laser light scattering and/or carry out the quantitative measurement of the particle diameter of emulsion droplet by the spectrophotometric turbidity measurement of UV/VIS in dissolve medium.Those of ordinary skill in the art can utilize and understand any of these methods.
According to pharmaceutical composition of the present invention preferably liquid or semisolid at ambient temperature.And, for example, by pharmaceutical composition (is adsorbed onto to solid carrier particle, silicon dioxide, calcium silicates or Magnesiumaluminumsilicate) upper to obtain the powder without mobility, can this powder be filled in hard capsule or be pressed into tablet, and these pharmaceutical compositions can be changed into solid dosage forms.Referring to, for example, US2003/0072798, its disclosure is incorporated in full by reference.Therefore, term " dissolving " is herein interpreted as describing active pharmaceutical ingredient (API), and it is dissolved in liquid solution or is scattered in equably in solid carrier.Can form and use the dosage form of encapsulation form.
The present invention is preferably incorporated in lipid surfactant excipient (for example, above-mentioned lipophile and hydrophilic surfactant active's combination in any) and has the lower API dissolving.Therefore, the fusing point of surfactant used is to determine that resulting composition will be liquid or a semisolid factor at ambient temperature.Especially preferred compositions of the present invention is liquid oral unit dosage forms, more preferably, be for example filled into, in hard or soft capsule,, gelatin or non-gelatine capsule, those capsules of for example being made by cellulose, carrageenin or Pul (pollulan).Those of ordinary skill in the art know the encapsulation techniques of the pharmaceutical preparation based on lipid.Because delivery system of the present invention described herein and preparation are not limited to any encapsulation method, so without concrete encapsulation techniques is further discussed.
Can be by the conventional art preparation for the medicament carrier system based on lipid according to medicament carrier system of the present invention and pharmaceutical preparation.At the typical method of preparing for the preferred carrier system of the present invention, weigh lipophilic surfactant component and add in suitable rustless steel container, then weigh hydrophilic surfactant active's component, and add in container together with other components.In a preferred method, can for example first hydrophobic drug be added, in lipophilic surfactant component (, oleic acid), and add hydrophilic surfactant active's component after dissolving completely.Under any circumstance, the mixing of component can be by being used homogenize blender or other high-shear devices and high temperature (especially using in high-melting-point surfactant situation) to complete to guarantee that all components is even liquid state before or after interpolation medicine.
In weighing hydrophobic drug and adding the lipid mixture of merging, preferably at high temperature continue to mix, until make homogeneous solution.Can make preparation degassed, then be encapsulated in soft or hard capsule.In some cases, can utilize suitable jacketed vessel to make to fill preparation keeps, under high temperature, being beneficial to processing.Equally, in some cases, homogeneous solution can filter (for example,, by 5 micron filters) before incapsulating.
Turn back to now testosterone and send, pharmaceutical composition of the present invention can be suitable for testosterone treatment.Testosterone is male's main endogenous androgen.Interstitial cell (Leydig cell) in testis produces about 7mg testosterone every day, cause serum-concentration approximately 300 to about 1100ng/dL.Women's ovary and adrenal gland be synthetic testosterone also, but its amount is in gonad function normal male viewed approximately 1/10th.Great majority (>=98%) circulation testosterone just has biological activity when being attached to sex hormone binding globulin and albumin and only discharging with free form.Therefore, term " dissociates " to be defined as and is not for example attached to, biomolecule, cell and/or the lipidic matrix of preparation of the present invention as herein described, or be not limited in wherein.Conventionally, " dissociating " as herein described medicine refers to the medicine that can contact the metabolic enzyme circulating in serum.
Although should not limit the invention to sending of testosterone or its any certain esters, find that TU provides unique chemistry and physical characteristic, it is preferably used in some embodiments.The inventor recognizes that the undecylate of testosterone especially can produce the bioavailability of comparing the viewed excellence of use other equivalent esters (for example, testosterone enanthatas ester (TE)).
And, the use of TU and the ratio relevant (table 1) of the significantly lower serum DHT:T of reported values (comprising the oral formulations of TU) of comparing other forms of T substitute in preparation of the present invention.Testosterone or testosterone interact with androgen receptor after changing into DHT by the effect of 5α-reductase.DHT is a kind of than the more effective androgen of testosterone, and some scientists think that the rising of DHT level has increased the risk of carcinoma of prostate.By this way, the invention provides another unexpected advantage of comparing other known testosterone delivery vehicle.
Table 1: viewed in response to the serum DHT of T-substitute and the comparison of DHT:T ratio by various route of administration
1atkinson, LE, Chang, Y-L and Synder, PJ. (1998) Long-term experience with testosterone replacement through scrotal skin.In:Testosterone:Action, Deficiency and Substitution
Particular of the present invention will be set forth in unrestricted embodiment.According to instruction of the present invention, table 2 provides the composition details of the several formulations of TU.In order to calculate, 1mg T is equivalent to 1.58mg T-undecylate.
The composition details (mg/ capsule and % by weight) of table 2 be based on each ' the about 800mg filling weight of 00' hard gelatin capsule.But, under the testosterone ester amount that is less than about 100mg/ capsule, can for example, adjust in proportion preparation for the less total filling weight that allows to use less hard gelatin capsule (, ' No. 0' or while needing, more ting model).
Equally, should it is evident that those of ordinary skill in the art, for example, in a kind (, lipophile, hydrophilic etc.) many (if not all) surfactant can exchange with another surfactant from one species.Therefore,, although table 1 has been listed the preparation that comprises oleic acid, those of ordinary skill in the art will be appreciated that other lipophilic surfactants (for example, above cited those) also may be applicable to.Similarly, comprise Cremophor RH40(HLB=13 although table 1 has been listed) preparation, those of ordinary skill in the art will be appreciated that other hydrophilic surfactant actives (for example, above listed those) may be applicable to.Borage oil, Oleum menthae, BHT and ascorbyl palmitate can substitute or be removed with the similar material of chemical property.
table 2
1milligram weight is rounded up to nearest integer; 800 (± 10%)
2±8mg
According to the preferred formulation of the TU in of the present invention being filled into " 00 " number capsule be:
preparation A
| Composition | Mg/ capsule | %,w/w |
| Testosterone undecanoate | 158.3 | 19.8 |
| Oleic acid | 413.1 | 51.6 |
| Cremophor?RH40 | 128.4 | 16.1 |
| Borage seed oil | 80.0 | 10 |
| Oleum menthae | 20.0 | 2.5 |
| BHT | 0.2 | 0.03 |
| Amount to | 800 | 100 |
preparation B
Next will describe according in the body of preparation of the present invention and external performance data.But the scope of the invention should not be restricted to the particular formulations of studying in following examples or embodiment.
the embodiment 1-odd-numbered day is studied
Research preparation B is once a day or the odd-numbered day pharmacokinetic curve of administered twice every day after the male of hypogonadism.By research design become a kind of open-label, odd-numbered day administration, continuously, the pharmacokinetic that intersects.Give written informed consent postscript, recruit the male of 12 hypogonadisms, and all 12 experimenters are completing research.The preparation B that every experimenter accepts every daily dose is as follows:
1.200mg T (with TU form) QD, that is, and 2 capsules/dosage
2.200mg T (with TU form) BID (100mg/ dosage), that is, and 1 capsule/dosage
3.400mg T (with TU form) BID (200mg/ dosage)
After 5 minutes, dosage is applied to experimenter (breakfast QD, and breakfast and dinner BID) with the form of capsule on the feed.
Table 3 provides the relevant PK parameter of research:
ainstitute's indicating value of half-life and peak time is intermediate value and scope.
bdosage is to represent with T equivalent.Each TU capsule contains 158.3mg TU, its corresponding 100mg T equivalent.
Average serum T concentration (C in 24 hours sections after administration
avg) represent that the serum T level of all schemes of studying just increases, wherein scheme 3 obtains optimum response (C
avg385ng/dL).That in this research, evaluates never exceeds Upper Limit of Normal Value (, 1100ng/dL) in response to the viewed average serum T of oral T-ester formulation peak concentration.Although some indivedual experimenters' C
maxt value exceeds upper limits of normal, but in these peaks the overwhelming majority be 1200 to 1400ng/dL.The experimenter's of any treatment group C
maxall do not exceed 1500ng/dL.
Serum T half-life (the T of scheme 1 and 2
1/2) intermediate value is about 15 hours, and the T of scheme 3
1/2it is 8 hours.In each scheme, serum DHT concentration increases together with serum T level.The average DHT:T ratio (R during all being measured by liquid chromatography-mass spectrography (LC/MS/MS)
avg) slightly exceed normal range (, 0.03-0.1), but not remarkable clinically.
Produce the most satisfied result with 200mgT equivalent by the TU of BID administration together with food, wherein 75% experimenter reaches serum T C
avghigher than the subnormal gonad function of 300ng/dL(normal limit).Similarly, the average serum T that 75% experimenter reaches (, 0.03-0.1ng/dL) within normal range.Do not reach C
avgfor those experimenters of 300ng/dL are at least more than 200ng/dL, show that the increase slightly of TU dosage will be effective oral T alternative medicine to these experimenters.
In the time of data in baseline correction serum T, observe most subjects serum T increase consistent with DHT concentration and with T-ester dosage indifference, wherein the dosage of oral TU is linear excellent.Although DHT:T ratio raises slightly, any rising is all considered as not remarkable clinically.The relatively equivalent preparation of other T esters (for example, TE), observes between the experimenter of this preparation variability little.And, in " BID " dosage regimen, 12 hours AUC zero differences during average serum T peak concentration or AM and PM administration.
About safety, although reported that headache is a kind of side effect, in each therapeutic scheme, in an experimenter, report does not have side effect.During research, there is not serious adverse events or death, and there is no an experimenter because of the research of adverse events premature interruption.Therefore, all adverse events are considered as to moderate strength event.
embodiment 2-research on the seven
Research preparation B is applied to acute tolerance and the stable state serum pharmacokinetic curve after the male of hypogonadism for twice with two doses in every day.Research design is become to the pharmacokinetic (checking food effect in a group) of a kind of open label, repeat administration, intersection.
Give written informed consent postscript, recruit the male of 29 hypogonadisms, wherein 24 have completed research.The scheme that every the experimenter who completes research accepts preparation B is as follows:
1.7 daily dose 600mg T(are with TU form), BID(300mg/ dosage), that is, and 3 capsules/dosage
2.8 daily dose 400mgT(are with TU form), BID(200mg/ dosage)
Starting feed (breakfast and dinner) after 30 minutes, with capsule form, dosage is applied to experimenter, but at the 8th day, AM dosage was used on an empty stomach.
T exposes peak value (C
max) and T always expose (AUC) to endogenous baseline T proofread and correct after and dosage proportional.T peak concentration (T
max) time occur in approximately 4 hours after each treatment administration.Equally, the serum-concentration of TU and DHTU rises and declines in dosing interval, wherein the concentration initial and that finish of dosing interval be less than TU peak concentration 20% and be less than 25% of DHTU peak concentration.For every kind for the treatment of, because endogenous T produces, baseline T concentration reduces gradually.Observe with promoting sexual gland hormone be subject to exogenous T gradually and lasting inhibition consistent, therefore cause the generation minimizing of endogenous T.Suppress at least partly the maintenance removing phase of 14 days.
Again, serum T pharmacokinetics does not show the diurnal variation of serum T concentration.PM administration (using about 8PM greatly) obtains using with AM (using about 8AM greatly) similarly concentration-time curve (Fig. 1).Because the concentration similarity between AM and PM administration (assessment in scheme 1), so be satiated with food from scheme 2() 12-hour PK data for predicated response exactly in the 200mg T(with the administration of BID mode the form with TU) the whole day PK curve of 24 hours.Analog result shows that (a) is based on AUC, the serum T C of 77% experimenter in 24 hours sections
avgwithin gonad function normal range, therefore meet 75% the performance requirements of current FDA to T substitute products; And (b) there is no experimenter's a C
maxexceed 1500ng/dL, this exceeds current FDA standard, for T substitute products, is less than 85% experimenter's C
maxbe greater than 1500ng/dL.Therefore, the usefulness terminal also specifying with current FAD is consistent, there is no experimenter's C
maxexceed 2500ng/dL and be less than 5% the experimenter's that studies C
maxwithin the scope of 1800-2500ng/dL.It should be noted that these results are all to obtain without any dose titration in the situation that.
Table 4 provides the stable state AM of T and the comparison of PM pharmacokinetics under with BID administration:
Using TU together with high fat diet produces and use similar serum T-concentration-time curve together with standard diet.On the contrary, in empty stomach situation, using TU causes serum T to expose (C
maxand AUC) decline more than 50% (table 5).In all cases, find the C of observation
maxwith the C calculating
avgbetween strong correlation, show with the specific C of oral T-ester formulation targeting
avgcan cause the peak value T level of expecting after administration.
DHT concentration is followed the trail of T concentration, although DHT concentration is only the 11-34% of T concentration.T shows slightly non-linear to the conversion of DHT, increase with the concentration ratio speed less than T.In the time that T concentration is the highest, DHT/T ratio minimum, and starting before TU treatment, DHT/T ratio is about 0.1, and during treating, under stable state, average ratio is 0.24 and in approximately 0.1 to 0.35 scope, depends on the sampling time after Orally administered TU.
Average estradiol concentration is about 11pg/mL starting before oral TU treatment, and the scope of the 7th day of various treatments (concentration before administration) in 19pg/mL to 33pg/mL scope.Stable state estradiol concentration before administration is about 20-30pg/mL.
the research of embodiment 3-surrounding
Also study preparation B with determine when to the male of hypogonadism with every day the dosage of twice 200mg T(with the form of TU) when (, 2 capsules/dosage) treatment 28 days, reach the required time of stable state.Research design is become to the pharmacokinetic of a kind of open label, repeat administration.
Giving written informed consent postscript, recruiting the male of 15 hypogonadisms, all male have completed research.Each experimenter accepts the 200mg T(of two doses every day with the form of TU), continue 28 days.
For each experimenter, the research arrangement of the 32nd day " the 28th day " continuous P K is sampled day.Therefore, each dosage-be obedient to experimenter accepts the 400mg T(of 31 daily doses altogether with TU form) (, 200mg T, BID), and last AM administration 200mgT(is with TU form).With capsule form application dosage, wherein instruct experimenter start feed (breakfast and dinner) after 30 minutes, take medicine.
Table 6 provides the relevant PK data of research:
Within normal range, 86.7% experimenter reaches serum T C
avg, there is no an experimenter's C
maxconcentration is greater than 1800ng/dL, and 13.3% experimenter's C only
maxconcentration is greater than 1500ng/dL.(note: carry out this research during do not carry out dose titration so that experimenter within the scope of target usefulness and safety).In tested preparation, be obviously longer than to T only or with the TU institute reported values of the preparation oral of prior art in response to the T half-life of TU.For example, in the clinical research of the oral TU preparation consistent with the present invention described herein, observe and eliminate the half-life (α phase) probably for approximately 5 hours, and disclosed serum T curve after prior art preparation based on oral administration TU, estimated value the chances are its half (, 2 to 3 hours).Use oral TU preparation of the present invention, observe equally long elimination (, the last eventually) half-life of 29 hours.But the generation of endogenous T is subject to the inhibition of using of exogenous T, wherein only limited inhibition occurs in 3 days of beginning, and the continued treatment that needs 5-7 days reaches maximum and suppresses.
To treatment the 7th day, T and DHT concentration reached stable state.The T of the 3rd day and the concentration of DHT are greater than the 5th day, and this shows for the exogenous T using, and needs a period of time to suppress endogenous T and produces, and therefore realizes the stable state in response to oral TU.Really the 276ng/dL from treatment is suppressed to the 108ng/dL after the supplementary T treatment of 28 days by endogenous T level, to add exogenous T.
But, significantly, once serum T in response to twice oral TU and reaching after stable state every day, is observed serum T response along with the time does not slightly drop to do not decline (, after continuous T U administration, without the trend towards lower serum T level).For example, the C of the 15th day
avgthe substantially similar C observing for the 28th day
avg(Fig. 2).By contrast, it is reported that the oral TU preparation of oral this area is in time towards lower T meansigma methods (Cantrill, J.A.Clinical Endocrinol (1984) 21:97-107).In the male of the hypogonadism with oral TU preparation for treating known in the art, it is reported the first day of the male treatment of the serum T response ratio hypogonadism observed after treatment in 4 weeks the little about 30%-of observed value wherein major part there is primary hypogonadism form and therefore the baseline values of serum T is low (for example, <100ng/dL), therefore, T reduces only to be explained by suppressing endogenous T.
Serum DHT concentration is followed the trail of T concentration closely, and DHT and DHT/T value have increased by 4 to 7 times during treating.Average DHT/T ratio during the dosing interval of 12 hours is 0.245, although the value during dosing interval can be in average maximum ratio 0.380 to the scope of average minimum scale 0.131.In 36 hours that interruption is treated with oral TU, DHT concentration turns back to the front level for the treatment of.But T concentration can not turn back to the front level for the treatment of soon, be obviously can not reverse soon because endogenous T produces/discharge the inhibition being subject to.
The concentration of estradiol (E2) shows single and little by little increases to stable state, and this also can reaching in treatment on the 7th day.E2 concentration also shows the systematic change that administration interim tracking T changes.The average C of E2
max, C
avgand C
minrespectively 30.6pg/mL, 22.0pg/mL and 15.5pg/mL.In 36 hours that interruption is treated with oral TU, E2 concentration turns back to the front level for the treatment of.
Average C when T stable state (the AM administration of the 28th day)
max, C
avgand C
minconcentration is respectively 995ng/dL, 516ng/dL and 199ng/dL.The intermediate value Tmax of T occurs in after administration 5.0 hours.C
minaverage out to C
max23.5%, obtaining the index of oscillation is 156%.Only the experimenter of about half has been assessed the elimination half-life of T, and those experimenters' intermediate value is 18.4 hours (average T
1/2it is 29 hours).
the research of embodiment 4-food effect
The research of any effect of the pharmacokinetics of the male's of hypogonadism dietary fat to preparation B is that a kind of open label, two center, five are to crossing research.The removing after date of 4-10 days, by 300 mg T(475mg TU of single dose, 3 preparation B capsules) be applied to 16 and there is the horizontal 205.5+25.3 ng/dL(of serum baseline T meansigma methods+SE, 23-334.1ng/dL) the male of hypogonadism.Making that experimenter is random accepts medicine or feed was accepted medicine after 30 minutes on an empty stomach, meals contain～and 800 calories and there is the fat (% by weight) of specified quantitative: utmost point low fat (6-10%); Low fat (20%); " normally " dietary fat (30%); Or higher fatty acid (50%)." normally " meals are defined as a kind of comparator for statistical (, with reference to meals) setly.After collection drug administration, the serial blood sample of 24 hours is to determine serum testosterone and dihydrotestosterone (DHT) level by liquid chromatography-mass spectrography (LC/MS/MS).
Find in response to the pharmacokinetic parameter (table 7, Fig. 3-5) of the serum T of single oral high dose TU and low fat and normal fatty diet similar-in fact bioequivalence (, 90% confidence interval is between 85-125%).In the time that normal and high fat diet compare, also observe similar serum T PK parameter.Although high fat diet produces higher serum T response (although statistically not occurring different), but when with normal fatty diet relatively time, the average proportions of least square meansigma methods between 70-143%-significant difference is not <30% clinically.
1c
avgwith AUC
0-∞/ τ calculates (τ=dosing interval=12 hour, BID administration)
The transmutability of PK reaction seems after administration for the first time, or start after the oral TU of dosage several times the highest, and along with treatment continues and reduces.Therefore, any impact of serum T PK parameter is seemed is all inapparent to the dietary fat within the scope of low-normal-Gao during long term administration.This with 7 days treatment (embodiment 2) and 30 days treatments (embodiment 3) PK result consistent, wherein, in the repeat administration of oral TU is studied, the PK in different meals situations still shows C
maxwith C
avgthe similar results [form of 200 mg T(with TU used in two kinds of researchs), BID] distributing.
Relatively normal fatty diet (, with reference to meals), do not taking food or taking food under utmost point low fat, low fat or high fat diet, after oral TU, the statistics of viewed serum T response relatively discloses: between low fat or high fat diet and normal meals, under p<0.05 level, there is not significant difference statistically.On the contrary, in the serum T PK parameter producing from normal meals significantly different (lower) of using with the oral TU of SEDDS dosage form on an empty stomach or while having taken food utmost point low fat breakfast.Therefore, the fat content of the meals of using together with preparation of the present invention can be different from " normally " substantially, and this does not have appreciable impact to obtained T level clinically.Therefore, allow the motility of the every meal of experimenter and the dietary habit of every day, this point be known oral TU preparation before this can not.Oral TU preparation known in the art can not reach any significant serum T level so far under empty stomach state.
the test of embodiment 5-In Vitro Dissolution
External stripping research of carrying out preparation of the present invention evaluate with body in the dependency of viewed PK curve.In first research, the dissolution of research preparation B.For relatively, comprise Andriol
(in each soft capsule, comprising the 40mg TU being dissolved in Oleum Ricini and lauric acid propylene glycol ester mixture).Under TU with dose,equivalent substantially, study, that is, and 1 preparation B capsule (158.3mg TU) and 4 Testocaps soft capsules (4 × 40mg=160mg TU).In simulated intestinal fluid (FeSSIF) medium of the state of being satiated with food, study stripping (that is, TU discharges from each self-preparing agent), described medium is simulated the intestinal juice being stimulated by meals.FeSSIF contains sodium hydroxide, glacial acetic acid, potassium chloride, lecithin and sodium taurocholate.Final emulsion is adjusted to pH5.0.
In table 8 and 9, the data of oblatio show that preparation of the present invention discharges about 40%TU and after 4 hours, discharges approximately 60% of whole capsule starting in 30 minutes.But, for
whole 4 hours seldom or there is no out (1%) of drug release.Main Differences the TU stripping of observing from these two kinds of preparations can be attributed at least partly and in preparation B, have hydrophilic surfactant active (for example, Cremophor RH40).By contrast, Andriol
only contain oil (Oleum Ricini) and lipophilic surfactant (lauric acid propylene glycol ester).
In second research, make preparation A carry out similar test, but use 5%Triton X100 kaliumphosphate buffer (pH6.8) as dissolution medium.Result provides in following table 10.In this research, in the beginning of stripping 15 minutes, from preparation of the present invention, discharge 98%TU, and again because the existence of hydrophilic surfactant active Cremophor RH40 is beneficial to this Fast Stripping in the nature of things and TU discharges.
In another embodiment of the present invention, pharmaceutical composition disclosed herein can be suitable for alleviating some the tactful side effect for male contraception equally.For example, the male contraception based on progesterone suppresses in fact lutropin (LH) and follicle stimulating hormone (FSH), and therefore Inhibit sperm occurs, and causes clinical azoospermia (be defined as continuous 2 months and be less than approximately 100 ten thousand sperms/mL seminal fluid).But, use progesterone and there is equally the non-required side effect that reduces significantly stable state level of serum testosterone.
For example, in this case, preferably provide the progesterone preparation that simultaneously contains testosterone or testosterone derivative (for example, TU).More preferably, provide according to pharmaceutical preparation of the present invention, it comprises progesterone-present in an amount at least sufficient to suppress in fact LH and produce-and testosterone of FSH.In some embodiments, pharmaceutical preparation described in oral delivery once a day.
Preparation of the present invention can provide and discharge the preparation extending, testosterone can be delivered in serum in several hours.In fact, between 3 and 7 hours, preferably, exceed 4,5 or 6 hours according to the half-life of serum testosterone of the present invention.By contrast, the serum half-life of male's testosterone is considered in the scope of 10 to 100 minutes.
Not bound by theory or restriction, thinks on the one hand, preparation of the present invention can be by strengthening civilian Chinese medicine by the lymphoid absorption of intestinal but not absorbed to reach these effects by Portal circulation.On the other hand, not bound by theory or restriction again, thinks that de-ester required time causes the T half-life of more growing by using the ester of testosterone.
Oral dose of the present invention can be by needing approximately every 12 hours of the experimenter of testosterone treatment once oral, to maintain the level of serum testosterone needing.In a more preferred, oral dose is by needing approximately every 24 hours of the experimenter of testosterone treatment once oral.Usually incite somebody to action, " required " testosterone levels is those levels of finding in the people experimenter without testosterone deficiency disease being characterized by.
Although the present invention is described in conjunction with its specific embodiments, should be appreciated that and can further revise and this description is intended to contain following all changes of the present invention, purposes or change.Conventionally, the principle of the invention and comprise belong to the known or conventional convention in correlation technique of the present invention and can be used for above-described basic feature and within the scope of appended claims to those variations of the present invention.
Claims (15)
1. a combination of oral medication, it comprises the testosterone undecanoate being dissolved in carrier, described carrier comprises at least one lipophilic surfactant and at least one hydrophilic surfactant active with total lipophilic surfactant and the ratio (w/w) of total hydrophilic surfactant active in about 6:1 to 3.5:1 scope, described compositions is once a day or twice of every day provide the testosterone concentration of the stable state average serum within the scope of approximately 300 to about 1100ng/dL, the described compositions that the testosterone undecanoate of wherein said dissolving comprises 18 to 22 % by weight after Orally administered.
2. combination of oral medication as claimed in claim 1, wherein said at least one hydrophilic surfactant active comprises Cremophor RH40(polyoxyethylene glycerol-trihydroxy-stearate).
3. combination of oral medication as claimed in claim 1, wherein said at least one lipophilic surfactant comprises oleic acid.
4. combination of oral medication as claimed in claim 1, wherein said testosterone undecanoate is dissolved in the carrier that does not basically contain ethanol.
5. combination of oral medication as claimed in claim 1, described at least one hydrophilic surfactant active that it comprises 15 to 17 % by weight.
6. combination of oral medication as claimed in claim 1, described at least one lipophilic surfactant that it comprises 50 to 55 % by weight.
7. the dosage form of a testosterone undecanoate, it comprises the testosterone undecanoate being dissolved in carrier, described carrier comprises at least one lipophilic surfactant and at least one hydrophilic surfactant active, described dosage form once a day or twice of every day by oral administration to suffering from after the experimenter of hypogonadism or its symptom, stable state average serum within the scope of approximately 300 to about 1100ng/dL testosterone concentration is provided, avoids exceeding the C of 2500ng/dL simultaneously
maxthe appearance of value.
8. the application of pharmaceutical composition in the medicine of preparation treatment testosterone deficiency disease or its symptom, described application comprises the pharmaceutical composition of the Orally administered effective dose of experimenter to suffering from testosterone deficiency disease or its symptom, described pharmaceutical composition comprises the testosterone undecanoate being dissolved in carrier, described carrier comprises at least one lipophilic surfactant and at least one hydrophilic surfactant active with total lipophilic surfactant and the ratio (w/w) of total hydrophilic surfactant active in about 6:1 to 3.5:1 scope, so that the testosterone concentration of the stable state average serum within the scope of approximately 300 to about 1100ng/dL to be provided.
9. purposes as claimed in claim 8, wherein said compositions is used once a day.
10. purposes as claimed in claim 8, wherein said compositions administered twice every day.
11. 1 kinds of combination of oral medication, it comprises:
A.18-22 the testosterone undecanoate of the dissolving of % by weight;
B.50-55 at least one lipophilic surfactant of % by weight; And
C.15-17 at least one hydrophilic surfactant active of % by weight;
D.10-15 the borage seed oil of % by weight and the mixture of Oleum menthae.
12. compositionss as claimed in claim 11, wherein said hydrophilic surfactant active is Cremophor RH40.
13. compositionss as claimed in claim 11, wherein said lipophilic surfactant is oleic acid.
14. compositionss as claimed in claim 11, wherein said lipophilic surfactant and described hydrophilic surfactant active's ratio is about 4:1.
15. 1 kinds of combination of oral medication, it comprises:
A.19.8 the testosterone undecanoate of the dissolving of % by weight;
B.51.6 the oleic acid of % by weight;
C.16.1 the Cremophor RH40 of % by weight;
D.10 the borage seed oil of % by weight;
E.2.5 the Oleum menthae of % by weight; And
F.0.03 the BHT of % by weight.
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| Application Number | Title | Priority Date | Filing Date |
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| CN201410005248.7A Pending CN103830245A (en) | 2010-04-12 | 2010-04-12 | Oral testosterone ester formulation and application of same in preparation of drug used for treating testosterone deficiency |
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1686143A (en) * | 2005-04-04 | 2005-10-26 | 浙江大学 | Hendecanoic acid testosterone self emulsified soft capsule composition and its preparation method |
| CN101217963A (en) * | 2005-04-15 | 2008-07-09 | 克劳拉斯医疗有限公司 | Pharmaceutical delivery systems for hydrophobic drugs and compositions comprising same |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1686143A (en) * | 2005-04-04 | 2005-10-26 | 浙江大学 | Hendecanoic acid testosterone self emulsified soft capsule composition and its preparation method |
| CN101217963A (en) * | 2005-04-15 | 2008-07-09 | 克劳拉斯医疗有限公司 | Pharmaceutical delivery systems for hydrophobic drugs and compositions comprising same |
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