CN103817322B - PSAu Core-shell structure material and preparation method thereof and application - Google Patents
PSAu Core-shell structure material and preparation method thereof and application Download PDFInfo
- Publication number
- CN103817322B CN103817322B CN201410064532.1A CN201410064532A CN103817322B CN 103817322 B CN103817322 B CN 103817322B CN 201410064532 A CN201410064532 A CN 201410064532A CN 103817322 B CN103817322 B CN 103817322B
- Authority
- CN
- China
- Prior art keywords
- core
- aqueous solution
- shell
- psau
- amino
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Abstract
The invention discloses a kind of PSAu Core-shell structure material and preparation method thereof and application.This has the material of nucleocapsid structure, is also PSAu, is made up of shell and core; The material forming shell is gold; The material forming core is polystyrene microsphere PS ball; Be connected by the PEI that described band is amino between shell with core; Wherein, the surfactant of band amino is connected by silicon oxygen bond or physisorption with between core, is connected by positive and negative electrostatic force with between shell; Described core is positioned at the center of described shell, and is entirely surrounded by described shell.This PSAu core-shell material and co-culture of cells, within the scope of finite concentration, 0-90 μ g/ml can promote the growth of MSC cell.
Description
Technical field
The present invention relates to a kind of PSAu Core-shell structure material and preparation method thereof and application.
Background technology
Nano material, due to the character of its uniqueness, receives much concern as skin effect, small-size effect, quantum size effect, macro quanta tunnel effect etc.Wherein the nanogold particle (AuNPs) of various shape is becoming study hotspot in recent years.Because its scale topography is easy to control synthesis, high, the good biocompatibility of chemical stability, is easy to carry out the photoelectric property of finishing and its uniqueness, as the surface plasma body resonant vibration (SPR) of AuNPs, the character such as SERS (SERS), make it have a wide range of applications in biological medicine.AuNPs has and reports widely in bio-imaging technology, bio-sensing, cancer diagnosis, therapy application, drug delivery etc.
From the Ancient books of China and Japan, can find that gold can be used as medicine, especially goldleaf.Formal row goldleaf being classified as food additives of the nineteen eighty-three World Health Organization (FAO/WHO) the food additive regulations committee (CCFA) the 16 meeting (Hague, Detch meeting).Also propose in recent work more both domestic and external, nm of gold is at some difficult diseases for the treatment of, as senile dementia (DingbinLiuetal.AHighlySensitiveGold-Nanoparticle-BasedAs sayforAcetylcholinesteraseinCerebrospinalFluidofTransgen icMicewithAlzheimer'sDisease.AdvancedHealthcareMaterials, 2012, 1, 90 – 95), cosmetic applications (PatelAnuradhaetal.OVERVIEWONAPPLICATIONOFNANOPARTICLESIN COSMETICS.AsianJournalofPharmaceuticalSciencesandClinica lResearch, 2011, Vol.1, Issue2, 40 – 55) and organizational project (MichalShevachetal.Nanoengineeringgoldparticlecomposite bersforcardiactissueengineering.J.Mater.Chem.B, 2013, DOI:10.1039/c3tb20584c) important application is had in the middle of.The prospect that AuNPs likely becomes future drugs causes people's interest widely.
In addition, due to developing rapidly of nano science, excite people and study the huge enthusiasm of various nano particle for organism and cytosis.Wherein there is great attraction about the research of nano particle on the toxicity of cell and the impact of Promote cell's growth, have a large amount of article reports in recent years about the work of this one side.
In the middle of these reports, we can see that gold nano grain has the effect of potential promotion cell proliferation, therefore we can utilize this effect of gold nano grain, realize some and be difficult to fast breeding that is that breed or benign cell, as liver cell, pancreaticβ-cell, stem cell etc.This medicinal significant for AuNPs.Therefore find a kind of easier, that cost is lower method rapidly and efficiently increased realizing seed cell on the basis of original cell seed particularly important for organizational project.
Summary of the invention
The object of this invention is to provide a kind of PSAu Core-shell structure material and preparation method thereof and application.
The material with nucleocapsid structure provided by the invention, is also PSAu, is made up of the surfactant of shell, core and band amino;
Form the material of described shell for gold;
The material forming described core is polystyrene microsphere;
Described shell is with amino surfactant to be connected with between core by described; Wherein, the surfactant of described band amino is connected by silicon oxygen bond or physisorption with between core, is connected by positive and negative electrostatic force with between shell.
In above-mentioned material, the particle diameter of described core is 200nm-3.2 μm, is specially 250nm, 300nm, 3.2 μm, 300nm-3.2 μm or 250-300nm;
The thickness of described shell is 0-30nm, is specially 16nm, 20nm, 25nm, 16-20nm or 20-25nm, and the thickness of described shell is not 0;
The amino surfactant of described band is selected from (3-mercaptopropyi) trimethoxy silane, (3-mercaptopropyi) triethoxysilane, vinyltrimethoxy silane, octyl group trimethoxy silane, 3-aminopropyl triethoxysilane, 3-aminopropyl trimethoxysilane and at least one of N, N-bis-in (amino-ethyl acyl Methylethyl) octadecylamine and PEI;
Described have in the material of nucleocapsid structure, and the mass concentration of gold is 0-90 μ g/ml, and being specially 0-50 μ g/ml, is more specifically 10-25 μ g/ml, and is not 0.
Above-mentioned core-shell material prepares by the following method and obtains.
Preparation provided by the invention has the method for the material of nucleocapsid structure, comprises the steps:
1) by the aqueous solution of aforementioned polystyrene microballoon and be describedly with the aqueous solution of amino surfactant to stir after the surface amination carrying out polystyrene microsphere reacts 20-40 minute, centrifugal collecting precipitation, gained is precipitated Eddy diffusion in water, add the aqueous solution of the gold nano grain as seed, after stirring carries out reaction 0.5-1.5 hour, the gold nano grain that centrifugal removing is unnecessary, collecting precipitation obtains PSAuNP particle;
2) by step 1) gained PSAuNP particle Eddy diffusion in water, then adds the mixing of growth-promoting media, reducing agent, protective agent and ammoniacal liquor and carries out the growth response of golden shell, react complete drying obtain described in there is the material of nucleocapsid structure.
The step 1 of said method) in, the concentration of the aqueous solution of polystyrene microsphere is 0-1.5mg/mL, is specially 1mg/mL, and concentration is not 0;
The concentration of the aqueous solution of the surfactant that described band is amino is 0-2mg/mL, be specially 1mg/mL, and concentration is not 0;
In above-mentioned surface amination reactions steps, the time is 30 minutes;
The mass ratio of the surfactant that described band is amino and polystyrene microsphere is 0-140:1, is specially 120:1, and the quality of the surfactant of band amino is not 0;
In described centrifugal collecting precipitation step, rotating speed is 5000-10000r/min, is specially 5000r/min, 9000r/min, 10000r/min or 5000-9000r/min; Time is 15-20min;
The mass ratio of the described gold nano grain as seed and PS ball is 0-50:1, and the quality of seed gold grain is not 0, is specially 10-30:1; The particle diameter of described gold nano grain is 2-5nm;
Described stirring is carried out in reactions steps, and the time is 1 hour.
In described step 1), the aqueous solution as the gold nano grain of seed prepares in accordance with the following steps and obtains:
Be the HAuCl of 2% by 125 μ L mass percentage concentration
4the aqueous solution joins 15ml containing in protectant aqueous solution, stirs after 10 minutes, then adds 1ml containing 0.0013gNaBH
4the aqueous solution carry out reduction reaction after 6 hours, water-soluble after product being dried to dry powder, obtain the described gold nano grain aqueous solution as seed.
Wherein, described protective agent is selected from least one of 2-Pyrrolidone, METHYLPYRROLIDONE, polyethylene glycol, polypropylene glycol and polyvinylpyrrolidone (K-30); The molecular weight of described polyethylene glycol is 200-900g/mol; The molecular weight of polypropylene glycol is 400-800g/mol.
Step 2) in, described growth-promoting media is according to ShiW.; SahooY.; SwihartM.andPrasadP.GoldNanoshellsonPolystyreneCoresforC ontrolofSurfacePlasmonResonance.Langmuir, 2005,21, method that 1610-1617 provides preparation and obtaining, the method is specially: by the reactant aqueous solution of the aqueous solution of potash and gold chloride after 30 minutes lucifuge deposit 24 hours and obtain;
Wherein, the concentration of the aqueous solution of described potash is specially 0.25-0.30g/L, is specially 0.27g/L;
The concentration of the aqueous solution of described gold chloride is specially 5mM;
The amount ratio of the aqueous solution of described potash and gold chloride is specially 0.05g:15ml;
Described reducing agent is selected from least one in sodium borohydride, ascorbic acid, hydroxylamine hydrochloride, hydrazine hydrate and formaldehyde;
Described protective agent is selected from least one of 2-Pyrrolidone, METHYLPYRROLIDONE, polyethylene glycol, polypropylene glycol and polyvinylpyrrolidone; Wherein, the molecular weight of described polyethylene glycol is 200-900g/mol; The molecular weight of polypropylene glycol is 400-800g/mol;
The mass percentage concentration of described ammoniacal liquor is 10-35%, is specially 25%;
The volume ratio of described growth-promoting media and polystyrene microsphere is 100-200:1, is specially 100:1;
The volume ratio of the aqueous solution of described growth-promoting media, reducing agent, protectant aqueous solution and ammoniacal liquor is 200:2:2:1;
In the growth response step of described golden shell, temperature is 0-50 DEG C, is specially 25 DEG C; Time is 8-15 hour, is specially 12 hours.
In addition, the material of what the invention described above provided have nucleocapsid structure promotes in preparation the application in the product of the mescenchymal stem cell propagation of people also to belong to protection scope of the present invention.Wherein, described promotion cell proliferation comprises: have the material of nucleocapsid structure and mescenchymal stem cell co-incubation in culture medium of described people by described;
In described Dual culture step, described in there is the concentration of gold element in described culture medium in the material of nucleocapsid structure be specially 0-90 μ g/ml, be more specifically 0-50 μ g/ml, then be specially 10-25 μ g/ml, and be not 0;
Described culture medium is specially α-MEM culture medium.
The above-mentioned PSAu Core-shell structure material prepared promotes the method for cell proliferation, specifically can comprise the steps: that the PSAu Core-shell structure material after by freeze drying is dissolved in culture medium, be configured to certain density solution, after cell 24 hours is adherent, add PSAu solution and co-culture of cells.
The above-mentioned PSAu nucleocapsid structure prepared promotes that the function of cell proliferation has concentration dependent, and its concentration is 0-90 μ g/ml, and the larger facilitation of size is better.
The spherical layering Nano composite granules of the one that PSAu core-shell material provided by the invention is made up of golden shell and PS core.It is when the PS ball of 200nm-3.2 μm forms PSAu nucleocapsid structure that the present invention finds nanogold particle to be fixed on diameter, the basis keeping cell primary characteristic can effectively strengthen the multiplication capacity of mescenchymal stem cell, delay MSC cell ageing, promote the growth of MSC cell.Experimental result shows, the larger promotion proliferation function for MSC cell of size of PSAu nucleocapsid structure is better, and extends facilitation enhancing in time.
Accompanying drawing explanation
Fig. 1 is the stereoscan photograph of the PSAu of preparation in embodiment 1.
Fig. 2 is the stereoscan photograph of the PSAu of preparation in embodiment 2.
Fig. 3 is the stereoscan photograph of the PSAu of preparation in embodiment 3.
Fig. 4 is the cytoactive curve after the PSAu nucleocapsid structure and MSC cytosis prepared in embodiment 1.
Fig. 5 is the cytoactive curve after the PSAu nucleocapsid structure and MSC cytosis prepared in embodiment 2.
Fig. 6 is the cytoactive curve after the PSAu nucleocapsid structure and MSC cytosis prepared in embodiment 3.
Detailed description of the invention
The experimental technique used in following embodiment if no special instructions, is conventional method.
The material used in following embodiment, reagent etc., if no special instructions, all can obtain from commercial channels.Following embodiment MSC cell used purchased from American Type culture collection institute (AmericanTypeCultureCollection, ATCC).
In following embodiment be used as the gold nano grain of seed the aqueous solution prepare all in accordance with the following steps and obtain:
Get 125 μ L2%HAuCl
4join 15ml containing PVP(K-30) in the aqueous solution of 0.0144g, stirs after 10 minutes, add 1ml and contain 0.0013gNaBH
4the aqueous solution, vigorous stirring, react stopping in 6 hours, by sample concentration, freeze drying becomes dry powder for subsequent use.The particle diameter of this gold nano grain is 2nm-5nm.
During use, gained dry powder is soluble in water according to proportioning again, as the nm of gold seed solution of preparation PSAu nucleocapsid structure.
The preparation of the PSAu Core-shell structure material of embodiment 1,250nm
1) PSAuNP particle is prepared:
Getting 200 μ L particle diameters is that the aqueous solution of 250nmPS (1mg/ml) joins in 24ml PEI (PEI) (1mg/ml) aqueous solution, slow stirring 30 minutes, the centrifugal 20min of 10000r/min washs the unnecessary PEI (PEI) of three removings, obtains PS/PEI precipitation.Then PS/PEI precipitation is resuspended in the 3ml aqueous solution, adds containing the 6ml aqueous solution of 0.006g as the gold nano grain of seed, slowly stir the gold seeds that after 1 hour, centrifugal removing is unnecessary, obtain PSAuNP particle;
2) PSAu Core-shell structure material is prepared:
Step 1) gained PSAuNP particle is resuspended in water; obtain 5ml solution; add 20ml growth-promoting media, 200 μ L5% reducing agent Vitamin C aqueous acids; the aqueous solution and the 100 μ L mass percentage concentration of 200 μ L1% protective agent METHYLPYRROLIDONEs are the ammoniacal liquor of 25%; in 25 DEG C of growth responses carrying out shell after 12 hours; the centrifugal 5min of 3000r/min washs three times, and freeze drying becomes dry powder, obtains PSAu Core-shell structure material provided by the invention.
The electromicroscopic photograph of this material as shown in Figure 1.As seen from the figure, in this material, the particle diameter of core is 250nm; The thickness of shell is 25nm;
And this material is made up of the surfactant of shell, core and band amino;
The material forming shell is gold;
The material forming core is polystyrene microsphere;
Be connected by the surfactant PEI (PEI) that band is amino between shell with core; Wherein, the surfactant PEI of band amino is connected by silicon oxygen bond or physisorption with between core, is connected by positive and negative electrostatic force with between shell.
The preparation of the PSAu nucleocapsid structure of embodiment 2,300nm.
1) according to the step of embodiment 1, be only that the particle diameter of PS in the aqueous solution of 250nmPS (1mg/ml) replaces with 300nm by particle diameter in step 1), will wherein " 10000r/min centrifugal 20min washing ", centrifugal rotational speed replaces with 9000r/min, time replaces with 15min, PSAuNP particle;
2) PSAu Core-shell structure material is prepared:
Step 1) gained PSAuNP particle is resuspended in water, obtain 5ml solution, add 40ml growth-promoting media, 40 μ L formaldehyde (aqueous solution), 400 μ L1%PVP(K30) and 200 μ L mass percentage concentration be the ammoniacal liquor of 25%, 25 DEG C of growth responses carrying out shell are after 12 hours, the centrifugal 5min of 2500r/min washs three times, freeze drying becomes dry powder, obtains PSAu Core-shell structure material provided by the invention.
The electromicroscopic photograph of this material as shown in Figure 2.As seen from the figure, in this material, the particle diameter of core is 300nm; The thickness of shell is 20nm;
And this material is made up of the surfactant of shell, core and band amino;
The material forming shell is gold;
The material forming core is polystyrene microsphere;
Be connected by the surfactant PEI (PEI) that band is amino between shell with core; Wherein, the surfactant PEI of band amino is connected by silicon oxygen bond or physisorption with between core, is connected by positive and negative electrostatic force with between shell.
The preparation of the PSAu Core-shell structure material that embodiment is 3,3.2 μm
1) according to the step of embodiment 1, only by the aqueous solution of 250nmPS in step 1) (1mg/ml), the particle diameter of PS replaces with 3.2 μm, the consumption of the aqueous solution of PS (1mg/ml) replaces with 800 μ L, and the consumption of PEI (1mg/ml) aqueous solution replaces with 30mL; In " the centrifugal 20min washing of 10000r/min ", centrifugal rotational speed replaces with 5000r/min; The 6ml aqueous solution containing 0.006g seed gold grain replaces with the 6ml aqueous solution containing 0.009g seed gold grain, obtains PSAuNP particle;
2) PSAu Core-shell structure material is prepared:
Step 1) gained PSAuNP particle is resuspended in water, obtain 5ml solution, add 80ml growth-promoting media, the 800 μ L2% hydroxylamine hydrochloride aqueous solution, 800 μ L1%2-pyrrolidone solution and 400 μ L mass percentage concentration are the ammoniacal liquor of 25%, 25 DEG C of growth responses carrying out shell are after 12 hours, the centrifugal 5min of 1500r/min washs three times, and freeze drying becomes dry powder, obtains PSAu Core-shell structure material provided by the invention.
The electromicroscopic photograph of this material as shown in Figure 3.As seen from the figure, in this material, the particle diameter of core is 3.2 μm; The thickness of shell is 16nm;
And this material is made up of the surfactant of shell, core and band amino;
The material forming shell is gold;
The material forming core is polystyrene microsphere;
Be connected by the surfactant PEI (PEI) that band is amino between shell with core; Wherein, the surfactant PEI of band amino is connected by silicon oxygen bond or physisorption with between core, is connected by positive and negative electrostatic force with between shell.
The PSAu Core-shell structure material of embodiment 4, embodiment 1 preparation is for promoting MSC cell proliferation
(1) MSC cell kind plate
When cell cover to reach 90% bottom blake bottle time vitellophag, digestive juice used is trypsase, then the centrifugal cell suspension being mixed with 25000/ml, and 100 μ L cell suspensions are inoculated in the 96 every holes of orifice plate, complete for inoculation orifice plate is placed in 37 DEG C and contains CO
2in the bio-incubator of 5%, 24 hour cells are adherent completely rear stand-by.
(2) the PSAu Core-shell structure material dry powder prepared by above-described embodiment 1 is dissolved in α-MEM culture medium, is prepared into highly concentrated solution.
Afterwards this concentrated solution is continued, with the dilution of α-MEM culture medium, to be prepared into five containing the dilution of Au mass concentration between 0-50 μ g/ml again, after cell 24 hours is adherent, adds 50 these dilutions of μ L, with co-culture of cells.
Acquired results as shown in Figure 4.
As seen from the figure, when the mass concentration of PSAu surface gold is at 0-50 μ g/ml, PSAu nucleocapsid structure has the effect of growth promoting effects for MSC cell, and facilitation is maximum when the mass concentration of gold is at 25 μ g/ml, and extends in time, and facilitation strengthens.
According to upper identical step, only PSAu Core-shell structure material prepared by embodiment 1 is replaced with PSAu Core-shell structure material prepared by embodiment 2, gained promote cell proliferation result as shown in Figure 5.
As seen from the figure, when the mass concentration of PSAu surface gold is at 0-50 μ g/ml, PSAu nucleocapsid structure has the effect of growth promoting effects for MSC cell, and facilitation is maximum when the mass concentration of gold is at 10 μ g/ml, and extends in time, and facilitation strengthens.
According to upper identical step, only PSAu Core-shell structure material prepared by embodiment 1 is replaced with PSAu Core-shell structure material prepared by embodiment 3, gained promote cell proliferation result as shown in Figure 6.
As seen from the figure, when the mass concentration of PSAu surface gold is at 0-40 μ g/ml, PSAu nucleocapsid structure has the effect of growth promoting effects for MSC cell, and facilitation is maximum when the mass concentration of gold is at 25 μ g/ml, and extends in time, and facilitation strengthens.
Claims (9)
1. a preparation method for PSAu Core-shell structure material, comprises the steps:
1) aqueous solution of surfactant amino with band for the aqueous solution of polystyrene microsphere is stirred carry out after surface amination reacts 20-40 minute, centrifugal collecting precipitation, gained is precipitated Eddy diffusion in water, add the aqueous solution of the gold nano grain as seed, after stirring carries out reaction 0.5-1.5 hour, collecting precipitation obtains PSAuNP particle;
2) by step 1) gained PSAuNP particle Eddy diffusion obtains suspension in water, add the growth response that golden shell is carried out in the mixing of growth-promoting media, the aqueous solution of reducing agent, protectant aqueous solution and ammoniacal liquor again, react complete drying and obtain described PSAu Core-shell structure material;
Described PSAu Core-shell structure material, is made up of the surfactant of shell, core and band amino;
Form the material of described shell for gold;
The material forming described core is polystyrene microsphere;
Described shell is with amino surfactant to be connected with between core by described; Wherein, the surfactant of described band amino is connected by silicon oxygen bond or physisorption with between core, is connected by positive and negative electrostatic force with between shell.
2. the preparation method of PSAu Core-shell structure material according to claim 1, is characterized in that: the particle diameter of described core is 200nm-3.2 μm;
The thickness of described shell is 0-30nm, and the thickness of described shell is not 0;
The amino surfactant of described band is selected from (3-mercaptopropyi) trimethoxy silane, (3-mercaptopropyi) triethoxysilane, vinyltrimethoxy silane, octyl group trimethoxy silane, 3-aminopropyl triethoxysilane, 3-aminopropyl trimethoxysilane and at least one of N, N-bis-in (amino-ethyl acyl Methylethyl) octadecylamine and PEI; Wherein, the molecular weight of described PEI is 10000-30000g/mol.
3. the preparation method of PSAu Core-shell structure material according to claim 2, is characterized in that: the molecular weight of described PEI is 25000g/mol.
4. the preparation method of PSAu Core-shell structure material according to claim 1, is characterized in that: described step 1) in, the concentration of the aqueous solution of polystyrene microsphere is 0-1.5mg/mL, and concentration is not 0;
The concentration of the aqueous solution of the surfactant that described band is amino is 0-2mg/mL, and concentration is not 0;
The mass ratio of the surfactant that described band is amino and polystyrene microsphere is 0-140:1, and the quality of the surfactant of band amino is not 0;
In described surface amination reactions steps, the time is 30 minutes;
In described centrifugal collecting precipitation step, rotating speed is 5000-10000r/min; Time is 15-20min;
The mass ratio of described gold nano grain and polystyrene microsphere is 0-50:1, and the quality of described gold nano grain is not 0; The particle diameter of described gold nano grain is 2-5nm;
Described stirring is carried out in reactions steps, and the time is 1 hour.
5. the preparation method of PSAu Core-shell structure material according to claim 4, is characterized in that:
Described step 1) in, the concentration of the aqueous solution of polystyrene microsphere is 1mg/mL;
The concentration of the aqueous solution of the surfactant that described band is amino is 1mg/mL;
The mass ratio of the surfactant that described band is amino and polystyrene microsphere is 120:1;
The mass ratio of described gold nano grain and polystyrene microsphere is 10-30:1.
6. the preparation method of PSAu Core-shell structure material according to claim 1, is characterized in that: described step 2) in, described growth-promoting media be by the reactant aqueous solution of the aqueous solution of potash and gold chloride after 30 minutes lucifuge deposit 24 hours and obtain;
Wherein, the concentration of the aqueous solution of described potash is 0.25-0.30g/L;
Described reducing agent is selected from least one in sodium borohydride, ascorbic acid, hydroxylamine hydrochloride, hydrazine hydrate and formaldehyde;
Described protective agent is selected from least one of 2-Pyrrolidone, METHYLPYRROLIDONE, polyethylene glycol, polypropylene glycol and polyvinylpyrrolidone; Wherein, the molecular weight of described polyethylene glycol is 200-900g/mol; The molecular weight of polypropylene glycol is 400-800g/mol;
The mass percentage concentration of described ammoniacal liquor is 10-35%;
The volume ratio of described growth-promoting media and polystyrene microsphere is 100-200:1;
The volume ratio of the aqueous solution of described growth-promoting media, reducing agent, protectant aqueous solution and ammoniacal liquor is 200:2:2:1;
In the growth response step of described golden shell, temperature is 0-50 DEG C; Time is 8-15 hour.
7. the preparation method of PSAu Core-shell structure material according to claim 6, is characterized in that: described step 2) in, the concentration of the aqueous solution of described potash is 0.27g/L;
The concentration of the aqueous solution of described gold chloride is 5mM;
The amount ratio of the aqueous solution of described potash and gold chloride is 0.05g:15ml;
The mass percentage concentration of described ammoniacal liquor is 25%;
The volume ratio of described growth-promoting media and polystyrene microsphere is 100:1;
In the growth response step of described golden shell, temperature is 25 DEG C; Time is 12 hours.
8., according to the preparation method of described PSAu Core-shell structure material arbitrary in claim 1-7, it is characterized in that: described step 1) in, the aqueous solution as the gold nano grain of seed prepares in accordance with the following steps and obtains:
Be the HAuCl of 2% by 125 μ L mass percentage concentration
4the aqueous solution joins 15ml containing in protectant aqueous solution, stirs after 10 minutes, then adds 1ml containing 0.0013gNaBH
4the aqueous solution carry out reduction reaction after 6 hours, water-soluble after product being dried to dry powder, obtain the described gold nano grain aqueous solution as seed.
9. the preparation method of PSAu Core-shell structure material according to claim 8, is characterized in that: described protective agent is selected from least one of 2-Pyrrolidone, METHYLPYRROLIDONE, polyethylene glycol, polypropylene glycol and polyvinylpyrrolidone;
Wherein, the molecular weight of described polyethylene glycol is 200-900g/mol; The molecular weight of polypropylene glycol is 400-800g/mol.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410064532.1A CN103817322B (en) | 2014-02-25 | 2014-02-25 | PSAu Core-shell structure material and preparation method thereof and application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410064532.1A CN103817322B (en) | 2014-02-25 | 2014-02-25 | PSAu Core-shell structure material and preparation method thereof and application |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103817322A CN103817322A (en) | 2014-05-28 |
| CN103817322B true CN103817322B (en) | 2016-04-13 |
Family
ID=50752772
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410064532.1A Expired - Fee Related CN103817322B (en) | 2014-02-25 | 2014-02-25 | PSAu Core-shell structure material and preparation method thereof and application |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103817322B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106935871A (en) * | 2015-12-31 | 2017-07-07 | 中国科学院化学研究所 | A kind of mesoporous PtAu nano materials of hollow ball-shape and preparation method and application |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104259474B (en) * | 2014-09-23 | 2016-09-07 | 中国科学院化学研究所 | A kind of preparation method of gold core-shell structured nanomaterials |
| CN105081345B (en) * | 2015-08-28 | 2017-04-12 | 东北大学 | Preparation method of ultra-thin metal shell layer nano-particles |
| CN106346019B (en) * | 2016-10-26 | 2018-06-12 | 华中科技大学 | A kind of micron chickens nucleocapsid of graphene support and preparation method thereof |
| CN106735180B (en) * | 2016-12-13 | 2019-04-26 | 哈尔滨理工大学 | A kind of method of polystyrene coating metal nano granule |
| CN109249018B (en) * | 2018-10-15 | 2019-09-06 | 华中科技大学 | A kind of self-assembling method of nanogold particle foam covering polystyrene microsphere |
| CN110804591B (en) * | 2019-11-07 | 2021-07-09 | 浙江大学 | Hematopoietic stem cell in-vitro culture system containing polymer micro-nano spheres and application |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5932104A (en) * | 1982-08-17 | 1984-02-21 | Konishiroku Photo Ind Co Ltd | Manufacture of ferromagnetic powder for surface treatment and magnetic recording medium |
| CN101767769A (en) * | 2009-12-31 | 2010-07-07 | 浙江大学 | Method for preparing silica/silver nuclear shell structure granules by using polyvinyl pyrrolidone (PVP) in an assistant way |
| CN102941118A (en) * | 2012-10-24 | 2013-02-27 | 中国航空工业集团公司北京航空材料研究院 | Au nanometer core-shell structure catalyst and preparation method thereof |
| CN103373702A (en) * | 2013-07-04 | 2013-10-30 | 天津大学 | Method for realizing growth of gold nanoparticles by utilizing block polymers as templates |
| CN103521753A (en) * | 2013-09-27 | 2014-01-22 | 中国科学院化学研究所 | Core-shell structure material and preparation method and application thereof |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8178202B2 (en) * | 2006-06-21 | 2012-05-15 | William Marsh Rice University | Nonconcentric nanoshells with offset core in relation to shell and method of using the same |
-
2014
- 2014-02-25 CN CN201410064532.1A patent/CN103817322B/en not_active Expired - Fee Related
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5932104A (en) * | 1982-08-17 | 1984-02-21 | Konishiroku Photo Ind Co Ltd | Manufacture of ferromagnetic powder for surface treatment and magnetic recording medium |
| CN101767769A (en) * | 2009-12-31 | 2010-07-07 | 浙江大学 | Method for preparing silica/silver nuclear shell structure granules by using polyvinyl pyrrolidone (PVP) in an assistant way |
| CN102941118A (en) * | 2012-10-24 | 2013-02-27 | 中国航空工业集团公司北京航空材料研究院 | Au nanometer core-shell structure catalyst and preparation method thereof |
| CN103373702A (en) * | 2013-07-04 | 2013-10-30 | 天津大学 | Method for realizing growth of gold nanoparticles by utilizing block polymers as templates |
| CN103521753A (en) * | 2013-09-27 | 2014-01-22 | 中国科学院化学研究所 | Core-shell structure material and preparation method and application thereof |
Non-Patent Citations (4)
| Title |
|---|
| The immobilization of hepatocytes on 24nm-sized gold colloid for enhanced hepatocytes proliferation;Gu HY etal;《Biomaterials》;20041231;第25卷;第3445-3451页 * |
| 刘大博等.PSAu核壳结构纳米催化剂的制备及性能研究.《材料工程》.2012,(第7期),第1-4、91页. * |
| 纳米金/壳聚糖仿生功能支架对角质形成细胞贴壁及增殖的影响;鲁双云;《中国组织工程研究与临床康复》;20081031;第12卷(第41期);第8011-8014页 * |
| 纳米金对人体表皮细胞增殖作用的体外实验研究;张敏娟;《中国美容医学》;20090930;第18卷(第9期);第1296-1298页 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106935871A (en) * | 2015-12-31 | 2017-07-07 | 中国科学院化学研究所 | A kind of mesoporous PtAu nano materials of hollow ball-shape and preparation method and application |
| CN106935871B (en) * | 2015-12-31 | 2019-07-09 | 中国科学院化学研究所 | A kind of mesoporous PtAu nano material of hollow ball-shape and the preparation method and application thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103817322A (en) | 2014-05-28 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103817322B (en) | PSAu Core-shell structure material and preparation method thereof and application | |
| CN101721372B (en) | Gold shell coated hollow mesoporous silicon dioxide spheres, method for preparing same and application thereof in tumor treatment | |
| CN101205420B (en) | Magnetic inorganic nano-particle/ordered meso-porous silica core-shell microspheres and preparation thereof | |
| CN103961705B (en) | The preparation of the hollow copper sulfide/poly-dopamine complex of modified with folic acid and application thereof | |
| Leal et al. | Effect of the surface treatment on the structural, morphological, magnetic and biological properties of MFe2O4 iron spinels (M= Cu, Ni, Co, Mn and Fe) | |
| CN101230208A (en) | Method for preparing gold nano-rod particles coated with silica layer | |
| CN103273080B (en) | A kind of nanometer golden flower and preparation method thereof and application | |
| CN102703411B (en) | Aramagnetic epoxy group mesoporous molecular sieve for immobilized biological enzymes, and preparation method thereof | |
| CN103787344A (en) | Water-soluble mesoporous silica nano-particle and preparation method and application thereof | |
| CN106409457A (en) | Preparation method and SERS application of monodispersed silver-shell magnetic microspheres | |
| CN103011177A (en) | Method for preparing mesoporous silicon dioxide nanometer material | |
| CN105963702A (en) | Drug delivery controlled-release system having tumor induced targeting capacity and preparation method of drug delivery controlled-release system | |
| CN106265514A (en) | A kind of doxorubicin hydrochloride magnetic nano particle and preparation method thereof | |
| Gong et al. | In vitro toxicity and bioimaging studies of gold nanorods formulations coated with biofunctional thiol-PEG molecules and Pluronic block copolymers | |
| CN103521753B (en) | A kind of Core-shell structure material and preparation method thereof and application | |
| CN102631876A (en) | Method for preparing core-shell-structured magnetic nano particles | |
| CN104844839B (en) | A kind of preparation method of magnetic fluorescence composite nanometer particle | |
| CN107522239A (en) | A kind of method based on gallic acid metallic ion coordination chemical regulation nano ferriferrous oxide decentralization and particle diameter | |
| CN105903979B (en) | A kind of Fe3O4The preparation method of@Au nucleocapsid functional materials | |
| CN103756229A (en) | Preparation method of thermosensitive composite gold nanoparticles | |
| CN103769216A (en) | Nano silver catalyst with thermosensitivity and magnetic property and preparation method thereof | |
| CN102532553B (en) | Preparation method of nano-silver penetrating hybridization sol | |
| CN104383920B (en) | The preparation method of a kind of MnOOH/Ag nano composite material and application thereof | |
| CN106215892A (en) | A kind of molecular engram microsphere and preparation method thereof and a kind of hydrogen peroxide enzyme inhibitor | |
| Li et al. | Multi-functional Fe3O4@ HMPDA@ G5-Au core-releasable satellite nano drug carriers for multimodal treatment of tumor cells |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20160413 Termination date: 20210225 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |