CN103808942A

CN103808942A - Protein reaction box

Info

Publication number: CN103808942A
Application number: CN201410041634.1A
Authority: CN
Inventors: 高社干; 朱小娟; 时振国
Original assignee: First Affiliated Hospital of Henan University of Science and Technology
Current assignee: First Affiliated Hospital of Henan University of Science and Technology
Priority date: 2014-01-28
Filing date: 2014-01-28
Publication date: 2014-05-21
Anticipated expiration: 2034-01-28
Also published as: CN103808942B

Abstract

The invention relates to a protein reaction box. The protein reaction box comprises a box body with the opening upper end and an upper cover, and further comprises a pressing mechanism I arranged on the box body and a pressing mechanism II arranged on the upper cover, wherein a distance between two pressing rods of the pressing mechanism can be adjusted through an adjustment device I; a distance between two pressing rods II of the pressing mechanism II can be adjusted through an adjustment device II. The protein reaction box provided by the invention can fix a PVDF (Polyvinylidene Fluoride) film inside an antibody solution, so that the PVDF film and antibodies are sufficiently subjected to contact reaction, and a reagent can be saved; the protein reaction box is particularly applicable to fixing the cut PVDF films with different sizes; no damage on a film exists; the adjustment method is simple, convenient and easy to operate; the pollution to an environment and air, caused by using a disposable plastic bag and a heat sealer, can be avoided; furthermore, the protein reaction box has the advantages of strong repeatability and smart structural design; the experiment cost can be greatly reduced.

Description

A kind of albumino reaction box

Technical field

The present invention relates to medical science, biomolecular science experiment apparatus technical field, be specifically related to a kind of albumino reaction box.

Background technology

Protein blot experiment (being Western blot) is medical science and the conventional experimental technique of molecular biosciences cytology, wherein the PVDF membrane after transferring film (pvdf membrane) or nitrocellulose filter (NC film) etc. are the committed step of experiment with antibody incubation, by albumen-antibody incubation technology be now: the pvdf membrane of the albumen that takes a turn for the better or NC film are placed in the polybag of cutting off, add after an antiantibody, catch up with after the interior gas of most bag or bubble, sealed with capper, be placed in 4 ℃ of refrigerator overnight incubation, two anti-also need again with new polybag, NC film or pvdf membrane to be hatched.But because pvdf membrane or NC film density are less than the density of antibody-solutions, easily floating in antibody-solutions, cause the albumen on pvdf membrane or NC film to contact inhomogeneous with antibody, in addition, pvdf membrane or the tender easy scuffing in surface of NC film, and when sealing, drive the bothersome effort of gas in bag, if residual gas also may cause pvdf membrane or NC film to contact insufficient with antibody, interference experiment result, the sensitivity of experiment is declined, the accuracy of impact experiment, make the failure of an experiment, waste of manpower, material resources, financial resources, and repeatedly use the disposable plastic bag also can be to environment.

Summary of the invention

The object of the invention is the deficiency for solving the problems of the technologies described above, a kind of albumino reaction box is provided, pvdf membrane or NC film can be fixed in antibody-solutions, make its abundant and antibody contact reaction, and can save reagent, but also reusable, avoid disposable plastic bag pollution on the environment, obviously reduce experimental cost.

The present invention is the deficiency solving the problems of the technologies described above, the technical scheme adopting is: a kind of albumino reaction box, comprise box body and the upper cover of upper end open, described albumino reaction box also includes the hold-down mechanism I being arranged on box body and is arranged at the hold-down mechanism II of upper cover, hold-down mechanism I comprises regulating device I, two toothed rack I, two connecting rod I and two compressor arm I, described regulating device I comprises rotating shaft I, gear I and thumb wheel I, rotating shaft I is located on box body one side sidewall, rotating shaft I can rotate freely, one end that described rotating shaft I is positioned at box body inside connects gear I, one end that rotating shaft I is positioned at box body outside connects thumb wheel I, the above and below of gear I is respectively equipped with a tooth bar I, two tooth bar I are all intermeshing with gear I, described two tooth bar I are all arranged on the sidewall of box body by chute, two tooth bar I are all parallel to box bottom setting, described two toothed rack I are all connected with compressor arm I by connecting rod I, two bottom surface settings that compressor arm I is parallel to each other and presses close to box body,

Described hold-down mechanism II comprises regulating device II, tooth bar II, connecting rod II and compressor arm II, regulating device II comprises rotating shaft II, gear II and thumb wheel II, rotating shaft II is through upper cover setting, and rotating shaft II can rotate freely, one end that rotating shaft II is positioned at upper cover inside arranges gear II, one end that rotating shaft II is positioned at upper cover outside arranges thumb wheel II, the both sides of gear II are provided with the two toothed rack II that are parallel to each other, two toothed rack II are all intermeshing with gear II, described two tooth bar II are all arranged on the lid of upper cover by chute, two toothed rack II are all connected with compressor arm II by connecting rod II, connecting rod II comprises two sections of bodies of rod, between two sections of bodies of rod, connect by spring, two compressor arm II are parallel to each other and perpendicular to the sidewall setting of box body.

beneficial effect

Albumino reaction box of the present invention, the film of the different materials such as pvdf membrane or NC film can be fixed in antibody-solutions, make its abundant and antibody contact reaction, and can save reagent, be particularly useful for the film of the difference size after fixing cutting, and to film not damaged, its control method is simple and easy to do, more avoided being suitable for the pollution that disposable plastic bag and heat sealing machine cause environment and air, and Reusability is strong, structural design is ingenious, greatly reduces experimental cost.

Accompanying drawing explanation

Fig. 1 is external structure schematic diagram of the present invention;

Fig. 2 is the structural representation of hold-down mechanism I of the present invention;

Fig. 3 is the structural representation of regulating device I of the present invention;

Fig. 4 is the structural representation of hold-down mechanism II of the present invention;

Fig. 5 is the structural representation of regulating device II of the present invention and connecting rod II;

Mark in figure: 1, box body, 2, upper cover, 301, regulating device I, 302, tooth bar I, 303, connecting rod I, 304, compressor arm I, 3011, rotating shaft I, 3012, gear I, 3013, thumb wheel I, 401, regulating device II, 402, tooth bar II, 403, connecting rod II, 404, compressor arm II, 4011, rotating shaft II, 4012, gear II, 4013, thumb wheel II, 4031, spring.

Embodiment

As shown in the figure: a kind of albumino reaction box, comprise box body 1 and the upper cover 2 of upper end open, described albumino reaction box also includes the hold-down mechanism I being arranged on box body 1 and is arranged at the hold-down mechanism II of upper cover 2, hold-down mechanism I comprises regulating device I 301, two toothed rack I 302, two connecting rod I 303 and two compressor arm I 304, regulating device I 301 is used for regulating two distances between compressor arm I 304, adjusts according to the pvdf membrane of different sizes.Described regulating device I 301 comprises rotating shaft I 3011, gear I 3012 and thumb wheel I 3013, rotating shaft I 3011 is located on box body 1 one side sidewalls, rotating shaft I 3011 can rotate freely, one end that described rotating shaft I 3011 is positioned at box body 1 inside connects gear I 3012, one end that rotating shaft I 3011 is positioned at box body 1 outside connects thumb wheel I 3013, stirring thumb wheel I 3013 can make gear I 3012 rotate, the above and below of gear I 3012 is respectively equipped with a tooth bar I 302, the engaging tooth that is positioned at the tooth bar I 302 of top arranges down, the engaging tooth that is positioned at the tooth bar I 302 of below arranges upward.Two tooth bar I 302 are all intermeshing with gear I 3012, and described two tooth bar I 302 are all arranged on the sidewall of box body 1 by chute.Two tooth bar I 302 are all fastened in chute, and tooth bar I 302 can not deviate from from chute, and tooth bar I 302 can be along the length direction displacement of chute.Two tooth bar I 302 are all parallel to box body 1 bottom surface and arrange, described two toothed rack I 302 all by connecting rod I 303 be connected with 304, two compressor arm I 304 of compressor arm I be separately positioned on two tooth bar I 302 away from end.Two compressor arm I 304 are parallel to each other and press close to box body 1 bottom surface and arrange;

Described hold-down mechanism II comprises regulating device II 401, tooth bar II 402, connecting rod II 403 and compressor arm II 404, and regulating device II 401 is used for regulating two distances between compressor arm II 404, adjusts according to the pvdf membrane of different sizes.Regulating device II 401 comprises rotating shaft II 4011, gear II 4012 and thumb wheel II 4013, rotating shaft II 4011 arranges through upper cover 2, and rotating shaft II 4011 can rotate freely, one end that rotating shaft II 4011 is positioned at upper cover 2 inside arranges gear II 4012, one end that rotating shaft II 4011 is positioned at upper cover 2 outsides arranges thumb wheel II 4013, stirring thumb wheel II 4013 can make gear II 4012 rotate, the both sides of gear II 4012 are provided with the two toothed rack II 402 that are parallel to each other, two toothed rack II 402 are all intermeshing with gear II 4012, described two tooth bar II 402 are all arranged on the lid of upper cover 2 by chute, two tooth bar II 402 are all fastened in chute, tooth bar II 402 can not deviate from from chute, and tooth bar II 402 can be along the length direction displacement of chute.Two toothed rack II 402 are all connected with compressor arm II 404 by connecting rod II 403, and connecting rod II 403 comprises two sections of bodies of rod, between two sections of bodies of rod, connect by spring 4031, and two compressor arm II 404 are parallel to each other and perpendicular to the sidewall setting of box body 1.

When use, the size of the pvdf membrane of operation as required, adjust two distances between compressor arm I 304 by regulating device I 301, stirring thumb wheel I 3013 rotates gear I 3012, 3012 rotations of gear I make two toothed rack I 302 relative displacements that are engaged with, just make 304 relative displacements simultaneously of two compressor arm I, shorten the distance between two compressor arm I 304, after adjustment finishes, pvdf membrane is placed on two compressor arm I 304, then adjust two distances between compressor arm II 404 by regulating device II 401, stirring thumb wheel II 4013 rotates gear II 4012, 4012 rotations of gear II make two toothed rack II 402 relative displacements that are engaged with, just make 404 relative displacements simultaneously of two compressor arm II, shorten the distance between two compressor arm II 404, after adjustment finishes, upper cover 2 is covered and makes two compressor arm I 304 and two compressor arm II 404 intersect to form " well " font, also can adjust the direction of upper cover 2, make two compressor arm I 304 parallel mutually with two compressor arm II 404, can compress two longer sides of pvdf membrane, pvdf membrane is clipped in the middle and is fixed, because two sections of bodies of rod of connecting rod II 403 connect by spring 4031, make the existing length adjustment of connecting rod II 403 space, pvdf membrane can be compressed again, albumino reaction box of the present invention, pvdf membrane can be fixed in antibody-solutions, make its abundant and antibody contact reaction, and can save reagent, be particularly useful for the pvdf membrane of the difference size after fixing cutting, and to film not damaged, its control method is simple and easy to do, more avoid being suitable for the pollution that disposable plastic bag and heat sealing machine cause environment and air, and Reusability is strong, structural design is ingenious, greatly reduce experimental cost.

Claims

1. an albumino reaction box, comprise box body (1) and the upper cover (2) of upper end open, it is characterized in that: described albumino reaction box also includes the hold-down mechanism I being arranged on box body (1) and is arranged at the hold-down mechanism II of upper cover (2), hold-down mechanism I comprises regulating device I (301), two toothed rack I (302), two connecting rod I (303) and two compressor arm I (304), described regulating device I (301) comprises rotating shaft I (3011), gear I (3012) and thumb wheel I (3013), rotating shaft I (3011) is located on box body (1) one side sidewall, rotating shaft I (3011) can rotate freely, described rotating shaft I (3011) is positioned at the inner one end of box body (1) and connects gear I (3012), rotating shaft I (3011) is positioned at the outside one end of box body (1) and connects thumb wheel I (3013), the above and below of gear I (3012) is respectively equipped with a tooth bar I (302), two tooth bar I (302) are all intermeshing with gear I (3012), described two tooth bar I (302) are all arranged on the sidewall of box body (1) by chute, two tooth bar I (302) are all parallel to box body (1) bottom surface and arrange, described two toothed rack I (302) are all connected with compressor arm I (304) by connecting rod I (303), two compressor arm I (304) are parallel to each other and press close to box body (1) bottom surface and arrange,

Described hold-down mechanism II comprises regulating device II (401), tooth bar II (402), connecting rod II (403) and two compressor arm II (404), regulating device II (401) comprises rotating shaft II (4011), gear II (4012) and thumb wheel II (4013), rotating shaft II (4011) arranges through upper cover (2), and rotating shaft II (4011) can rotate freely, rotating shaft II (4011) is positioned at the inner one end of upper cover (2) gear II (4012) is set, rotating shaft II (4011) is positioned at the outside one end of upper cover (2) thumb wheel II (4013) is set, the both sides of gear II (4012) are provided with the two toothed rack II (402) that are parallel to each other, two toothed rack II (402) are all intermeshing with gear II (4012), described two tooth bar II (402) are all arranged on the lid of upper cover (2) by chute, two toothed rack II (402) are all connected with compressor arm II (404) by connecting rod II (403), connecting rod II (403) comprises two sections of bodies of rod, between two sections of bodies of rod, connect by spring (4031), two compressor arm II (404) are parallel to each other and perpendicular to the sidewall setting of box body (1).