CN103805546B - Acinetobacter johnsonii AJ-3 bacterial strain and uses thereof - Google Patents
Acinetobacter johnsonii AJ-3 bacterial strain and uses thereof Download PDFInfo
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- 230000001580 bacterial effect Effects 0.000 title claims abstract description 49
- 241000122229 Acinetobacter johnsonii Species 0.000 title claims abstract description 30
- 239000010865 sewage Substances 0.000 claims abstract description 9
- 238000004321 preservation Methods 0.000 claims abstract description 8
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 abstract description 24
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- 239000011574 phosphorus Substances 0.000 abstract description 24
- 239000007788 liquid Substances 0.000 abstract description 18
- 238000000034 method Methods 0.000 abstract description 17
- 235000015097 nutrients Nutrition 0.000 abstract description 12
- 239000002351 wastewater Substances 0.000 abstract description 12
- 230000012010 growth Effects 0.000 abstract description 11
- BHEPBYXIRTUNPN-UHFFFAOYSA-N hydridophosphorus(.) (triplet) Chemical compound [PH] BHEPBYXIRTUNPN-UHFFFAOYSA-N 0.000 abstract description 5
- 230000008569 process Effects 0.000 abstract description 4
- 239000003446 ligand Substances 0.000 abstract description 3
- 238000000926 separation method Methods 0.000 abstract description 3
- 230000003044 adaptive effect Effects 0.000 abstract description 2
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- 230000000813 microbial effect Effects 0.000 abstract description 2
- 244000005700 microbiome Species 0.000 abstract description 2
- 229910052760 oxygen Inorganic materials 0.000 abstract description 2
- 239000001301 oxygen Substances 0.000 abstract description 2
- 241000894006 Bacteria Species 0.000 description 15
- 239000001888 Peptone Substances 0.000 description 15
- 235000015278 beef Nutrition 0.000 description 13
- 239000007787 solid Substances 0.000 description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 11
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 9
- 229920000388 Polyphosphate Polymers 0.000 description 8
- 229920000037 Polyproline Polymers 0.000 description 8
- 239000001205 polyphosphate Substances 0.000 description 8
- 235000011176 polyphosphates Nutrition 0.000 description 8
- 238000012360 testing method Methods 0.000 description 6
- 108090000623 proteins and genes Proteins 0.000 description 5
- 239000010802 sludge Substances 0.000 description 5
- 239000000126 substance Substances 0.000 description 5
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 4
- 239000008103 glucose Substances 0.000 description 4
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 description 3
- 229920001817 Agar Polymers 0.000 description 3
- 229930064664 L-arginine Natural products 0.000 description 3
- 235000014852 L-arginine Nutrition 0.000 description 3
- 229910002651 NO3 Inorganic materials 0.000 description 3
- 229920002472 Starch Polymers 0.000 description 3
- 230000004913 activation Effects 0.000 description 3
- 239000008272 agar Substances 0.000 description 3
- APUPEJJSWDHEBO-UHFFFAOYSA-P ammonium molybdate Chemical compound [NH4+].[NH4+].[O-][Mo]([O-])(=O)=O APUPEJJSWDHEBO-UHFFFAOYSA-P 0.000 description 3
- 229940010552 ammonium molybdate Drugs 0.000 description 3
- 235000018660 ammonium molybdate Nutrition 0.000 description 3
- 239000011609 ammonium molybdate Substances 0.000 description 3
- 238000010790 dilution Methods 0.000 description 3
- 239000012895 dilution Substances 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 238000009294 enhanced biological phosphorus removal Methods 0.000 description 3
- 238000012851 eutrophication Methods 0.000 description 3
- 238000000855 fermentation Methods 0.000 description 3
- 230000004151 fermentation Effects 0.000 description 3
- 229920000159 gelatin Polymers 0.000 description 3
- 235000019322 gelatine Nutrition 0.000 description 3
- 229910000037 hydrogen sulfide Inorganic materials 0.000 description 3
- 150000002475 indoles Chemical class 0.000 description 3
- 239000002054 inoculum Substances 0.000 description 3
- CEQFOVLGLXCDCX-WUKNDPDISA-N methyl red Chemical compound C1=CC(N(C)C)=CC=C1\N=N\C1=CC=CC=C1C(O)=O CEQFOVLGLXCDCX-WUKNDPDISA-N 0.000 description 3
- 230000009467 reduction Effects 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- 238000002798 spectrophotometry method Methods 0.000 description 3
- 239000008107 starch Substances 0.000 description 3
- 235000019698 starch Nutrition 0.000 description 3
- 241000589291 Acinetobacter Species 0.000 description 2
- 201000004384 Alopecia Diseases 0.000 description 2
- KRKNYBCHXYNGOX-UHFFFAOYSA-K Citrate Chemical compound [O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O KRKNYBCHXYNGOX-UHFFFAOYSA-K 0.000 description 2
- RWSOTUBLDIXVET-UHFFFAOYSA-N Dihydrogen sulfide Chemical compound S RWSOTUBLDIXVET-UHFFFAOYSA-N 0.000 description 2
- 108010010803 Gelatin Proteins 0.000 description 2
- 241000726221 Gemma Species 0.000 description 2
- ODKSFYDXXFIFQN-BYPYZUCNSA-N L-arginine Chemical compound OC(=O)[C@@H](N)CCCN=C(N)N ODKSFYDXXFIFQN-BYPYZUCNSA-N 0.000 description 2
- NHNBFGGVMKEFGY-UHFFFAOYSA-N Nitrate Chemical compound [O-][N+]([O-])=O NHNBFGGVMKEFGY-UHFFFAOYSA-N 0.000 description 2
- 108010080698 Peptones Proteins 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
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- 235000011852 gelatine desserts Nutrition 0.000 description 2
- 235000019319 peptone Nutrition 0.000 description 2
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- 238000012163 sequencing technique Methods 0.000 description 2
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- 230000001954 sterilising effect Effects 0.000 description 2
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- 239000001828 Gelatine Substances 0.000 description 1
- 238000003794 Gram staining Methods 0.000 description 1
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- 108010077895 Sarcosine Proteins 0.000 description 1
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 description 1
- YCUVUDODLRLVIC-UHFFFAOYSA-N Sudan black B Chemical compound C1=CC(=C23)NC(C)(C)NC2=CC=CC3=C1N=NC(C1=CC=CC=C11)=CC=C1N=NC1=CC=CC=C1 YCUVUDODLRLVIC-UHFFFAOYSA-N 0.000 description 1
- 241001052560 Thallis Species 0.000 description 1
- QGAVSDVURUSLQK-UHFFFAOYSA-N ammonium heptamolybdate Chemical compound N.N.N.N.N.N.O.O.O.O.O.O.O.O.O.O.O.O.O.O.O.O.O.O.O.O.O.O.O.O.[Mo].[Mo].[Mo].[Mo].[Mo].[Mo].[Mo] QGAVSDVURUSLQK-UHFFFAOYSA-N 0.000 description 1
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- OKKJLVBELUTLKV-UHFFFAOYSA-N methanol Natural products OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 1
- CXKWCBBOMKCUKX-UHFFFAOYSA-M methylene blue Chemical compound [Cl-].C1=CC(N(C)C)=CC2=[S+]C3=CC(N(C)C)=CC=C3N=C21 CXKWCBBOMKCUKX-UHFFFAOYSA-M 0.000 description 1
- 229960000907 methylthioninium chloride Drugs 0.000 description 1
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- 229920013639 polyalphaolefin Polymers 0.000 description 1
- 229920000137 polyphosphoric acid Polymers 0.000 description 1
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
Abstract
The invention belongs to field of environment microorganism <b>, does is </b> specifically related to a strain Acinetobacter johnsonii (<i>AcinetobacterLEssT.LTssT.L T/i><i>? johnsonii</i>) separation of AJ-3 bacterial strain, cultivation and the application in sewage dephosphorization thereof, be applied to the biological phosphate-eliminating of municipal sewage plant.Acinetobacter johnsonii of the present invention (<i>AcinetobacterLEssT.LTssT.L T/i><i>? johnsonii</i>) AJ-3 bacterial strain, deposit number is; CCTCC? M2014023, is preservation: China typical culture collection center, and preservation day is: on 01 16th, 2014.Does is the present invention a strain Acinetobacter johnsonii (<i>AcinetobacterLEssT.LTssT.L T/i><i>? johnsonii</i>) AJ-3 bacterial strain, this strain growth is quick, adaptive capacity to environment is strong, require low to dissolved oxygen, can grow fast in phosphorous artificial wastewater liquid nutrient medium, and by phosphorus ligands, dephosphorization efficiency by using is high and stable, reach 55% ~ 60.1%, non-secondary pollution.Therefore, AJ-3 bacterial strain can be applicable to phosphorous sewage disposal, is Microbial resources important on aqueous bio dephosphorization process, has a good application prospect.
Description
Technical field
The invention belongs to field of environment microorganism
,be specifically related to a strain Acinetobacter johnsonii (
acinetobacterjohnsonii) separation of AJ-3 bacterial strain, cultivation and the application in sewage dephosphorization thereof, be applied to the biological phosphate-eliminating of municipal sewage plant.
Background technology
The China Environmental State Bulletin display from 2010 to 2012,62 state control emphasis lake (reservoir) body eutrophication outstanding problems, East Sea immediate offshore area water-quality is extreme difference and serious pollution over nearly 3 years, all comprises phosphorus in main contamination index; And eutrophication will reduce the transparency of water body, produce obnoxious flavour and biotoxin, affect the health of people and animals, destroy species diversity in water body, impact tourism and shipping etc.; Therefore, the phosphorus ligands in water body is particularly important to preventing body eutrophication.
Current water body dephosphorized technology mainly divides two classes: chemical dephosphorization and biological phosphate-eliminating.Chemical dephosphorization is mainly chemical precipitation method, have simple to operate, clearance is high, the advantage such as stable; But the chemical sludge amount that this method exists, and dosage is large, processing costs is high, produce is large and be rich in organic matter, and the ultimate disposal of chemical sludge is also a problem.Biological phosphate-eliminating mainly utilizes polyP bacteria to the inrichment of phosphorus, the phosphorus in water body is effectively removed by the mode getting rid of residue rich phosphorous sludge, biological phosphate-eliminating has the advantages such as sludge output is few, save energy, working cost are lower, non-secondary pollution, is the phosphorus removing method that application is more at present.The sewage dephosphorization method mainly biological dephosphorization that extensive sewage work is conventional, wherein Enhanced Biological Phosphorus Removal method (EnhancedBiologicalPhosphorusRemoval, EBPR) there is sludge yield few, do not use the feature such as chemical substance and economical operation.
PolyP bacteria (PhosphorusAccumulataingOrganisms, PAOs) is not the concept in systematic bacteriology, is the general name to a quasi-microorganism with " excess suction phosphorus " feature.So-called " excess suction phosphorus ", refers to that they unnecessary phosphorus with the form of polyphosphoric acid salt storage in vivo, can be prepared against in poor environment, provide energy and nutrition.So the phosphorus content of some polyP bacteria thalline can reach more than 10% of dry weight.And because the phosphorus content in external environment can be made obviously to reduce in the process of " excess suction phosphorus ", polyP bacteria just becomes the main executive of biological removal of phosphorus in wastewater, plays a decisive role in biological phosphate-eliminating.
The present invention be sieved to from natural habitat one select good strains in the field for seed there is typical anaerobism characteristic polyP bacteria Acinetobacter johnsonii (
acinetobacterjohnsonii) AJ-3 bacterial strain, be intended to the polyP bacteria resource enriched, expand polyP bacteria strain library, for trade effluent, biological removal of phosphorus in wastewater provide bacterial classification.
Summary of the invention
The object of this invention is to provide plant height effect polyP bacteria Acinetobacter johnsonii and an application thereof.
Acinetobacter johnsonii of the present invention (
acinetobacterjohnsonii) AJ-3 bacterial strain, deposit number is; CCTCCM2014023, is preservation: China typical culture collection center, and preservation day is: on 01 16th, 2014.
Bacterial strain screening involved in the present invention is from the pedotheque of Zhejiang Normal University of Jinhua, Zhejiang Province city north gate.Adopt gradient dilution method to obtain pure bacterial strain, according to morphological specificity, physiological and biochemical property, 16SrDNA gene order identify AJ-3 bacterial strain be Acinetobacter johnsonii (
acinetobacterjohnsonii).
AJ-3 bacterial strain bacterium colony circle, white, neat in edge, protuberance, smooth moistening, translucent (accompanying drawing 1); Thalline is rod-short, Gram-negative, atrichia, without gemma (accompanying drawing 2).
The test of AJ-3 bacterial strain energy hydrolyzed starch, nitrate reduction and L-arginine decarboxylase is all positive, and can not utilize Citrate trianion, not liquefy gelatin, and the test of VP, methyl red, indoles, hydrogen sulfide, glucose fermentation is feminine gender (table 1); Strain growth temperature range is 15 DEG C ~ 40 DEG C, and optimum growth temperature is 28 DEG C, and suitable growth pH is 7.0 ~ 8.0.
16SrDNA sequencing analysis result shows, its base sequence total length is 1431bp(accompanying drawing 3), with Acinetobacter johnsonii (
acinetobacterjohnsonii) homology be 98%(accompanying drawing 4).
The condition that AJ-3 bacterial strain involved in the present invention can be used for the biological phosphate-eliminating of artificial wastewater is as follows: the glycerol stock 20 μ L being stored in-20 DEG C that goes bail for coats on the flat board of beef extract-peptone solid medium, choose single bacterium colony after 28 DEG C of cultivation 24h to mix in 0.3mL sterilized water, getting 100 μ L coats on the flat board of beef extract-peptone solid medium, getting 2 holes with punch tool (aperture 0.5cm) receives in 15mL beef extract-peptone liquid nutrient medium, be placed in 28 DEG C, in 170r/min shaking table, cultivate 24h(OD
600≈ 0.9) activation, make seed liquor; Access artificial wastewater liquid nutrient medium with the inoculum size of 8% again, temperature 28 DEG C, shaking table speed 170r/min, initial pH cultivate 9h under the condition of 7.2.Get 50mL bacteria suspension in the centrifuge tube of 50mL, with the centrifugal 5min of 12000r/min, get supernatant liquor and survey phosphorus concentration by the measuring method of total phosphorus, total phosphorus yield adopts ammonium molybdate spectrophotometric method, calculate dephosphorizing rate according to contrast, obtaining the highest dephosphorizing rate is 60.1%(accompanying drawing 6).
The present invention be a strain Acinetobacter johnsonii (
acinetobacterjohnsonii) AJ-3 bacterial strain, this strain growth is quick, and adaptive capacity to environment is strong, requires low, can grow fast, and by phosphorus ligands, dephosphorization efficiency by using is high and stable, reaches 55% ~ 60.1%, non-secondary pollution in phosphorous artificial wastewater liquid nutrient medium to dissolved oxygen.Therefore, AJ-3 bacterial strain can be applicable to phosphorous sewage disposal, is Microbial resources important on aqueous bio dephosphorization process, has a good application prospect.
Accompanying drawing explanation
Fig. 1 is the colony characteristics of AJ-3 bacterial strain.
Fig. 2 is the thalli morphology (× 1000) of AJ-3 bacterial strain.
Fig. 3 is AJ-3 bacterial strain 16SrDNA gene order.
Fig. 4 is the acinetobacter phylogenetic tree set up based on 16srDNA gene order.
Fig. 5 is the growth curve of AJ-3 bacterial strain.
Fig. 6 is the impact of liquid amount on AJ-3 bacterial strain dephosphorizing rate.
biological deposits is stated
Acinetobacter johnsonii (
acinetobacterjohnsonii) AJ-3 bacterial strain, deposit number is; CCTCCM2014023, is preservation: China typical culture collection center, and preservation day is: on 01 16th, 2014.
Embodiment
the separation screening of the efficient polyP bacteria Acinetobacter johnsonii AJ-3 bacterial strain of embodiment 1 and qualification
(1) substratum
Beef extract-peptone solid medium: extractum carnis 3g/L, peptone 10g/L, NaCl5g/L, agar 20g/L, pH7.0-7.2(liquid nutrient medium do not add agar);
Limit phosphorus (rich phosphorus) solid medium: get 30mL deionized water dissolving 8.372g3-morpholine propanesulfonic acid, 0.717gN-tri-(methylol) methylglycine, regulates pH to 7.4 with the KOH of 10mol/L, adds solution in the following order: 0.01mL1.830%FeSO
4solution (now joining), 5mL1.9mol/LNH
4cl, 1mL0.276mol/LK
2sO
4, 0.025mL0.02mol/LCaCl
22H
2o, 0.42mL1.25mol/LMgCl
26H
2o, 20mL2.5mol/LNaCl, 0.02mL trace element mixed solution (Ammonium Heptamolybdate 3 × 10
-6mol/L, H
3bO
34 × 10
-4mol/L, CoCl
23 × 10
-5mol/L, CuSO
410
-5mol/L, MnCl
28 × 10
-5mol/L, ZnSO
410
-5mol/L), 10mL1% glucose, 0.4mL1.686% VITMAIN B1,250 μ L6.928%K
2hPO
4(rich phosphorus adds 5mL)
,50mg para-totuidine is blue, adds water and is settled to 500mL, after filtration sterilization with sterilising treatment be dissolved with 20g agar and not solidified 500mL solution mixes and makes solid medium.
(2) method
Adopt gradient dilution partition method.Soil sampling 1.0g, adds the dilution of 9mL sterilized water, carries out 10
-1~ 10
-6gradient dilution, gets 10
-5diluent 100 μ L coats on beef extract-peptone solid medium flat board, cultivates 24h for 28 DEG C.The single bacterium colony of picking bacterium, carries out being separated, purifying; Be further purified bacterial strain and adopt method of scoring, the pure bacterial strain of gained is put in-20 DEG C of refrigerators and is preserved, for subsequent use.
With Lv Shi methylene blue staining, sudan black staining, blue hickie method and ammonium molybdate spectrophotometric method, bacterial strain is screened, after primary dcreening operation and multiple sieve, obtain the bacterial isolates (accompanying drawing 2) that a strain is numbered AJ-3 efficient dephosphorization.
Morphology, Physiology and biochemistry and 16SrDNA Sequence Identification are carried out to AJ-3 bacterial strain.
(3) result
Strain morphology feature: bacterium colony circle, white, neat in edge, protuberance, smooth moistening, translucent (accompanying drawing 1); Thalline is rod-short, and gramstaining is negative, atrichia, without gemma (accompanying drawing 2).
Bacterial strain physiological and biochemical property: the test of AJ-3 bacterial strain energy hydrolyzed starch, nitrate reduction and L-arginine decarboxylase is all positive, Citrate trianion can not be utilized, not liquefy gelatin, the test of VP, methyl red, indoles, hydrogen sulfide, glucose fermentation is feminine gender (table 1); Strain growth temperature range is 15 DEG C ~ 40 DEG C, and optimum growth temperature is 28 DEG C, and suitable growth pH is 7.0 ~ 8.0.
16SrDNA gene order (accompanying drawing 3): 16SrDNA gene sequencing analytical results shows, its base sequence total length is 1431bp, uses online Blast software to carry out homology analysis, chooses the strain construction phylogenetic tree (accompanying drawing 4) of 21 strain acinetobacters.Shown by Fig. 4, AJ-3 bacterial strain and Acinetobacter johnsonii (
acinetobacterjohnsonii) on same of evolutionary tree, two bacterial strain homologys are up to 98%.In conjunction with strain morphology feature, physiological and biochemical property, identify this bacterial strain be Acinetobacter johnsonii (
acinetobacterjohnsonii).
The physiological and biochemical test result of table 1AJ-3 bacterial strain
| Test subject | Result | Test subject | Result |
| Starch Hydrolysis | + | VP | - |
| Nitrate reduction | + | Methyl red | - |
| L-arginine decarboxylase | + | Indoles | - |
| Citrate trianion | - | Hydrogen sulfide | - |
| Gelatine liquefication | - | Glucose fermentation | - |
Note: "+" is positive, "-" is negative.
the growth curve of embodiment 2 Acinetobacter johnsonii AJ-3 bacterial strain measures
(1) bacterial strain:aJ-3 bacterial strain.
(2) substratum
Beef extract-peptone solid medium: same embodiment 1 of filling a prescription
Artificial wastewater liquid nutrient medium: sodium acetate 0.925g/L, peptone 0.1g/L, yeast extract paste 0.01g/L, NaCl0.05g/L, KH
2pO
40.0655g/L, MgCl
26H
2o0.1412g/L, CaCl
20.025g/L, pH7.0-7.2.
(3) method
The glycerol stock 20 μ L being stored in-20 DEG C that goes bail for coats on the flat board of beef extract-peptone solid medium, choose single bacterium colony after 28 DEG C of cultivation 24h to mix in 0.3mL sterilized water, getting 100 μ L coats on the flat board of beef extract-peptone solid medium, getting 2 holes with punch tool (aperture 0.5cm) receives in 15mL beef extract-peptone liquid nutrient medium, be placed in 28 DEG C, in 170r/min shaking table, cultivate 24h(OD
600≈ 0.9) activation, make seed liquor; Be equipped with in the 250mL Erlenmeyer flask of 100mL artificial wastewater liquid nutrient medium with the inoculum size access of 8% again, be placed in 28 DEG C, cultivate in 170r/min shaking table, measure OD every 1h
600value.
(4) result
After AJ-3 bacterial strain is activated, in artificial wastewater substratum, growth is fast, adaptable; In culturing process, do not occur obvious lag phase, 1h ~ 9h is logarithmic phase vegetative period, and 9h ~ 24h is in the stable growth phase (accompanying drawing 5) always.
embodiment 3 liquid amount is on the impact of Acinetobacter johnsonii AJ-3 bacterial strain biological phosphor-removing effect.
(1)
bacterial strain:aJ-3 bacterial strain.
(2) substratum
Beef extract-peptone solid medium: same embodiment 1 of filling a prescription;
Artificial wastewater liquid nutrient medium: same embodiment 2 of filling a prescription.
(3) method
The glycerol stock 20 μ L being stored in-20 DEG C that goes bail for coats on the flat board of beef extract-peptone solid medium, choose single bacterium colony after 28 DEG C of cultivation 24h to mix in 0.3mL sterilized water, getting 100 μ L coats on the flat board of beef extract-peptone solid medium, getting 2 holes with punch tool (aperture 0.5cm) receives in 15mL beef extract-peptone liquid nutrient medium, be placed in 28 DEG C, in 170r/min shaking table, cultivate 24h(OD
600≈ 0.9) activation, make seed liquor; Be equipped with in artificial wastewater liquid nutrient medium (liquid amount is respectively 40mL, 80mL, 100mL, 120mL, 150mL, 200mL) the 250mL triangular flask of different amount with the inoculum size access of 8% again, temperature 28 DEG C, shaking table speed 170r/min, initial pH cultivate 9h under the condition of 7.2.Get 25mL bacteria suspension in the centrifuge tube of 50mL, with the centrifugal 5min of 12000r/min, get supernatant liquor and survey phosphorus concentration by the measuring method of total phosphorus, total phosphorus yield adopts ammonium molybdate spectrophotometric method, calculates dephosphorizing rate according to contrast.
(4) result
Different liquid amount has a certain impact to AJ-3 bacterial strain phosphor-removing effect, when liquid amount is fill 120mL in 250mL triangular flask, and temperature 28 DEG C, shaking table speed 170r/min, cultivate 9h under artificial wastewater liquid nutrient medium (pH is 7.2), dephosphorizing rate can reach 60.1%; It is 80mL ~ 150mL(accompanying drawing 6 that AJ-3 bacterial strain is suitable for liquid amount).
Claims (2)
1. Acinetobacter johnsonii (
acinetobacterjohnsonii) AJ-3 bacterial strain, it is characterized in that: the deposit number of this bacterial strain is; CCTCCM2014023, is preservation: China typical culture collection center, and preservation day is: on 01 16th, 2014.
2. the purposes of Acinetobacter johnsonii AJ-3 bacterial strain according to claim 1, is characterized in that: this bacterial strain is used for the biological phosphate-eliminating of sewage.
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| CN103805546B true CN103805546B (en) | 2016-01-20 |
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| CN105586294B (en) * | 2016-01-07 | 2019-01-15 | 温州大学 | One plant of acinetobacter calcoaceticus and its application in phosphorus is denitrogenated in waste water |
| CN108359617B (en) * | 2017-12-29 | 2021-03-02 | 浙江双良商达环保有限公司 | Acinetobacter CL05 and application thereof in village and town sewage dephosphorization treatment |
| CN108774625B (en) * | 2017-12-29 | 2021-02-23 | 浙江双良商达环保有限公司 | Acinetobacter CL04 and application thereof in village and town sewage dephosphorization treatment |
| CN109022328B (en) * | 2018-09-05 | 2019-11-05 | 海南师范大学 | The application of one plant of polyP bacteria and its Polyphosphate kinase gene in sewage dephosphorization |
| CN109402008B (en) * | 2018-11-15 | 2021-09-14 | 中国农业科学院饲料研究所 | Acinetobacter TAT1-6A with indole degradation capacity and application thereof |
| CN110106097A (en) * | 2019-04-25 | 2019-08-09 | 黄山市益天士生物科技有限公司 | Accelerate the strain enrichment procedure of reparation eutrophication water |
| CN110106108A (en) * | 2019-04-25 | 2019-08-09 | 黄山市益天士生物科技有限公司 | Method for improving purifying water body breeding colony speed |
| CN113462595B (en) * | 2021-06-18 | 2022-08-23 | 江南大学 | Efficient phosphorus accumulating strain obtained by ARTP mutagenesis |
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| CN86106566A (en) * | 1985-08-09 | 1988-04-20 | 吉斯特-布罗卡迪斯公司 | New lipolytic enzyme and the application in descaling composition thereof |
| CN1050560A (en) * | 1989-09-25 | 1991-04-10 | 武汉大学 | Produce the novel method of beet pulp single cell protein |
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| CN86106566A (en) * | 1985-08-09 | 1988-04-20 | 吉斯特-布罗卡迪斯公司 | New lipolytic enzyme and the application in descaling composition thereof |
| CN1050560A (en) * | 1989-09-25 | 1991-04-10 | 武汉大学 | Produce the novel method of beet pulp single cell protein |
Non-Patent Citations (1)
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| 约氏不动杆菌（Acinetobacter johnsonii）CH-1的分离及其聚磷特性的研究;连丽丽等;《微生物与人类健康科技论坛论文汇编》;20091231;146-149 * |
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