CN103805498B - Grabbing device and there is the instrument of extraction biologically active substance of this grabbing device - Google Patents
Grabbing device and there is the instrument of extraction biologically active substance of this grabbing device Download PDFInfo
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- CN103805498B CN103805498B CN201310371016.9A CN201310371016A CN103805498B CN 103805498 B CN103805498 B CN 103805498B CN 201310371016 A CN201310371016 A CN 201310371016A CN 103805498 B CN103805498 B CN 103805498B
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- compression bar
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
- C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/145—Extraction; Separation; Purification by extraction or solubilisation
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Abstract
The invention provides a kind of grabbing device for extracting in the instrument of biologically active substance, it is characterized in that described device comprises frame (1) and grasping mechanism, wherein said frame (1) has the casing board frame used corresponding to bar magnet method, described grasping mechanism comprises multiple thimble (5), compacting part (2) and compacting part drive unit, under the driving of compacting part drive unit, compacting part can move the synchronous backward in occurred level direction, thus drive be fixed thereon described multiple thimble generation locking phase near or away from movement, can leave or insert the projection on casing board four angles thus respectively.The casing board being placed with sleeve pipe can lift and the original shape of maintainance set tube sheet by the present invention, guarantees that casing board deformation does not occur.
Description
Technical field
The present invention relates to biological apparatus field.Concrete, the invention provides and extract for bar magnet method the grabbing device capturing casing board in the self-reacting device of biologically active substance, and provide the self-reacting device adopting the bar magnet method of described grabbing device to extract biologically active substance.
Background technology
Biologically active substance is extracted at multiple fields such as modern clinic medical diagnosis on disease, transfusion safety, Forensic Identification, environmental microorganism detection, food safety detection, molecular biology researches, such as cell, and the biologically active substance such as protein, nucleic acid has vital role.Along with the development of bio-science, the method needing the extraction of high-throughout sample biologically active substance and full-automatic biologically active substance to extract and instrument.
Utilize paramagnetic particle method extract biologically active substance particularly active macromolecules be the technology that developed recently gets up.Paramagnetic particle method is that utilize can the magnetic bead of specific recognition cell, protein or nucleic acid, this magnetic bead can be combined specifically with nucleic acid molecule, then under the influence of a magnetic field, separate from the active macromolecules blood, animal tissues, food, pathogenic micro-organism equal samples.
The instrument utilizing paramagnetic particle method to extract biologically active substance can be divided into bar magnet method and suction method.Wherein bar magnet method is extracted is the technology that developed recently gets up, and its principle is that magnetic separating device is immersed liquid, makes the particle that can be attracted magnetically in mixture concentrate on separating device surface by the action of a magnetic field.Wherein in a kind of mode, magnetic separating device is a sleeve pipe, makes sleeve pipe produce magnetic by inserting bar magnet, attract the granular absorption that can be attracted magnetically in mixture to sleeve surface, when bar magnet is detached, sleeve pipe loses magnetism, and the particle be attracted magnetically departs from sleeve pipe.The flux of bar magnet method instrument for extracting nucleic acid has 8,16,32,96 passages usually, and relative to suction method, the advantage of bar magnet method is that the liquid that each walks does not remain, because bar magnet only takes away magnetic bead, transfers in the corresponding reacting hole of next step.Drive the mechanism of sleeve pipe or bar magnet motion that bar magnet can be driven to move up and down in sleeve pipe in bar magnet method instrument, and drive sleeve pipe move up and down in liquid sample or vibrate, contribute to the cracking of sample in leaching process, absorption, rinsing and purifying etc.
In bar magnet method instrument, sleeve pipe is the pipe with closed bottom and hollow tube chamber.Sleeve pipe can have such as elongated cylinder form.The material of sleeve pipe is normally nonmagnetic.The Choice and design of its material and thickness is for not weakened field or only affect magnetic field a little, but needs the magnetic field that ensures to produce the biological sample that can attract magnetic bead or band magnetic bead outward at sleeve pipe when inserting needle.
Extract in bar magnet method in the instruments and methods of biologically active substance and casing board can be adopted simultaneously to carry and multiple sleeve pipe is provided.Casing board can be fixed, capture and move by by the grasping mechanism in bar magnet method instrument, handles location and the movement of the sleeve pipe assembling or be fixed thereon.The grasping mechanism that current extraction apparatus adopts picks up this casing board by hook, easily cause this casing board generation deformation or produce displacement and make the sleeve pipe be placed in this casing board tilt or move in the horizontal direction, can not coordinate with bar magnet very well and produce magnetic, and often cause the failure of whole experiment, make these instruments be difficult to realize automatization.
Summary of the invention
The invention provides the grabbing device capturing casing board in a kind of self-reacting device for bar magnet method extraction biologically active substance.On the other hand, present invention also offers a kind of bar magnet method that utilizes efficiently comprising this grabbing device and extract the self-reacting device of biologically active substance.Device of the present invention and instrument can solve to be picked up casing board due to grasping mechanism by hook in prior art and easily causes this casing board generation deformation or produce displacement and make the sleeve pipe be placed in this casing board tilt or move in the horizontal direction, and can not coordinate the technical problem producing magnetic very well with bar magnet.
The present invention can be applicable to the multiple fields such as clinical disease diagnosis, transfusion safety, Forensic Identification, environmental microorganism detection, food safety detection, molecular biology research, for Isolation and purification biologically active substance, and such as cell, albumen and nucleic acid.
Concrete, the invention provides the grabbing device in a kind of instrument extracting biologically active substance for bar magnet method, it may be used for capturing the casing board used in bar magnet method.The instrument of bar magnet method extraction biologically active substance, equipment, articles for use, the middle casing board adopted of test kit (kit) have tetragonal dull and stereotyped seat usually, fix in the centre of described dull and stereotyped seat or the sleeve pipe of some amount is releasably set, such as 4x6 or 8x12 sleeve pipe.The dull and stereotyped seat of casing board has tetragonal frame, and four angles of this dull and stereotyped seat have can for the hollow projection of grasping mechanism contact.Described casing board can be commercially available normal sleeve plate.
The invention provides the grabbing device capturing casing board in a kind of instrument for bar magnet method extraction biologically active substance, it is characterized in that, described grabbing device comprises frame 1 and grasping mechanism, wherein said frame 1 has four frame frames of the square frame corresponding to casing board, described grasping mechanism comprises multiple thimble 5, compacting part 2 and compacting part drive unit, described compacting part 2 comprises first compression bar 21 and second compression bar 22 in the outside of the relative frame of two of being set in parallel in described frame 1, described multiple thimble 5 is arranged on the first compression bar 21 and the second compression bar 22 respectively,
Wherein, described first compression bar 21 and the second compression bar 22 are under the driving of described compacting part drive unit, can move the synchronous backward in occurred level direction, thus drive the described multiple thimbles be fixed thereon occur synchronous near or away from movement, can leave or insert the projection on casing board four angles thus respectively.
Of the present invention in one of them, the feature of described grabbing device is, described multiple thimble is four thimbles (51, 52, 53, 54), thimble 51 and 52 is arranged on the first compression bar 21, thimble 53 and 54 is arranged on the second compression bar 22, four thimbles (51, 52, 53, 54) position corresponds respectively to the projection on casing board four angles, compression bar 21 and 22 is under the driving of compacting part drive unit, can occur relative to away from or the moving of close motion, thus drive thimble (51, 52, 53, 54) synchronously insert respectively or leave the projection on casing board four angles, compress in the horizontal direction or unclamp casing board.
Of the present invention in one of them, the feature of described grabbing device is, described compacting part 2 is except above-mentioned first compression bar 21 and the second compression bar 22, also comprise the 3rd compression bar 23, second compression bar 22 is fixedly connected with by axle 6 the 3rd compression bar 23, by connection and the gearing of axle 6, the 3rd compression bar 23 and the second compression bar 22 synchronizing moving in the same way
Wherein, first compression bar 21 and the second compression bar 22 be arranged in parallel, be installed on outside the relative frame of two of described frame 1 respectively, first compression bar 21 and the 3rd compression bar 23 are arranged on the homonymy of one of them frame of framework 1, first compression bar 21 and the 3rd compression bar 23 can do synchronous backward and move under the driving of compacting part drive unit, and the first compression bar 21 and the second compression bar 22 do reverse sync and move thus.
Of the present invention in one of them, the feature of described grabbing device is, the middle part of described first compression bar 21 and the 3rd compression bar 23 has the first compression bar inclined-plane (213,214) and the 3rd compression bar inclined-plane (233,234) respectively.
Of the present invention in one of them, the feature of described grabbing device is, described compacting part drive unit comprises tensioning block 3, motor 4 and bearing 7, described tensioning block 3 comprises upper reel 31 and the lower shaft disk 32 at axle bed 33 and axle bed two ends, described head bearing 71 and lower bearing 72 are set in outside described upper reel 31 and lower shaft disk 32, the motor shaft that described axle bed 33 has power supply machine 4 passes and fixing hole 331
Wherein, upper reel 31 and lower shaft disk 32 can respectively by the inclined-planes (213 of head bearing 71 and lower bearing 72 and the first compression bar 21, 214) inclined-plane (233 of the and three compression bar 23, 234) come in contact, thus, when motor 4 drives tensioning block 3 to seesaw, the upper reel 31 of tensioning block 3 and lower shaft disk 32 extrude the first compression bar inclined-plane (213 respectively by head bearing 71 and lower bearing 72, 214) the and three compression bar inclined-plane (233, 234), promote the first compression bar 21 and the 3rd compression bar 23 thus and synchronous backward motion occurs, thus drive the thimble laying respectively at the first compression bar 21 and the second compression bar 22 occur relative away from or close motion.Of the present invention in one of them, described head bearing 71 and lower bearing 72 are rolling bearing.
Of the present invention in one of them, the feature of described grabbing device is, described compacting part drive unit also comprises point two guide rails 8 being located at the motor shaft both sides of motor 4, and tensioning block 3 is passed guide rail 8 by the hole 332 in its axle bed 33 and is slidably connected with guide rail 8.Of the present invention in one of them, the two ends of described guide rail 8 are erected on leading rail chair 81 and rear rail seat 82, leading rail chair 81 and rear rail seat 82 are fixed on the base plate 17 of frame outside framework by modes such as welding or screw threads, tensioning block 3 is slidably connected with guide rail 8 by the hole 332 in its axle bed 33, and can under the driving of motor 4, movable in the scope of leading rail chair 81 and rear rail seat 82.
Of the present invention in one of them, the feature of described grabbing device is, described frame 1 has outside framework 15, and the first compression bar 21 and the 3rd compression bar 23 of described compacting part drive unit and compacting part 2 are arranged in described outside framework 15.
Of the present invention in one of them, the feature of described grabbing device is, the frame of described frame 1 is set to, when the thimble 5 of grasping mechanism inserts or leaves casing board protruding, and the surface of frame of described frame 1 and the bezel contact of casing board the dull and stereotyped seat of clamping sleeve tube sheet.
Of the present invention in one of them, the feature of described grabbing device is, described frame 1 also has the compressing member 16 for clamping sleeve tube sheet in vertical direction, the surface level of the surface of described compressing member 16 and the frame of frame 1, be set at thimble 5(51,52,53,54) insert or leave casing board protruding time, its surface contacts dull and stereotyped seat of also clamping sleeve tube sheet with the plate face of casing board.
Of the present invention in one of them, the feature of described grabbing device is, described thimble is hard parts.Described thimble is the hard parts having anchorage force, and hollow projection on four angles can inserting the dull and stereotyped seat of casing board thus also provides enough holding powers, plays promptly and permanent sleeve tube sheet, and the effect of tensioning flat board seat.The material of described thimble can be metal, such as iron or stainless steel, aluminum products as aluminium alloy.The material of described thimble also can be nonmetallic, as hard plastics etc.
Present invention also offers and a kind ofly extract the instrument of biologically active substance for bar magnet method, it is characterized in that, described instrument comprise aforementioned for capture casing board grabbing device.Described instrument has the mechanism for supporting and control to extract in bar magnet method needle and the sleeve pipe used in biologically active substance, and its middle sleeve is assemblied on casing board regularly or removably.The described instrument for bar magnet method extraction biologically active substance can be the instrument for Isolation and purification nucleic acid.
Present invention also offers in the operation that the instrument that uses aforementioned grabbing device of the present invention or bar magnet method to extract biologically active substance extracts at bar magnet method biologically active substance for capturing and fixing the method for the casing board carrying sleeve pipe.
Grabbing device provided by the invention or the instrument adopting the biologically active substance of this grabbing device to extract compared with prior art more reliable and more stable.Device of the present invention and instrument have the hard thimble having anchorage force by adopting in grasping mechanism, can steadily firmly be lifted by the casing board being placed with sleeve pipe and fix the original shape of maintainance set tube sheet.And the design due to device of the present invention makes the casing board that is lifted and frame frame fit, make the further permanent sleeve tube sheet of frame thus guarantee that casing board deformation does not occur, thus ensure when adopting bar magnet method to extract biologically active substance, sleeve pipe energy good cooperation with bar magnet in casing board produces magnetic, attract the granular absorption that can be attracted magnetically in mixture to sleeve surface, for Isolation and purification biologically active substance, such as cell, albumen and nucleic acid, extensively and effectively can be applied in clinical disease diagnosis, transfusion safety, Forensic Identification, environmental microorganism detects, food safety detection, the multiple fields such as molecular biology research.Device of the present invention solves in prior art and easily causes this casing board generation deformation due to grasping mechanism or produce displacement and make the sleeve pipe be placed in this casing board tilt or move in the horizontal direction, the technical problem producing magnetic can not be coordinated very well with bar magnet, thus achieve the automatization of bar magnet method biologically active substance extraction apparatus.
Accompanying drawing explanation
By reading the detailed description done non-limiting example done with reference to the following drawings, other features, objects and advantages of the present invention will become more obvious:
Fig. 1-1 is the schematic perspective view of the first direction of a kind of grabbing device in accordance with a preferred embodiment of the present invention;
Fig. 1-2 is the exploded perspective view of the second direction of a kind of grabbing device in accordance with a preferred embodiment of the present invention;
Fig. 2-1 is the structural representation of the first compression bar in accordance with a preferred embodiment of the present invention;
Fig. 2-2 is the structural representation of the second compression bar in accordance with a preferred embodiment of the present invention;
Fig. 2-3 is the structural representation of the 3rd compression bar in accordance with a preferred embodiment of the present invention;
Fig. 2-4 is the assembling schematic diagram of the second compression bar in accordance with a preferred embodiment of the present invention and the 3rd compression bar;
Fig. 3 is the structural representation of tensioning block in accordance with a preferred embodiment of the present invention;
Fig. 4 is the structural representation of thimble in accordance with a preferred embodiment of the present invention;
Fig. 5 is the structural representation of the casing board that grabbing device in accordance with a preferred embodiment of the present invention captures;
Fig. 6 is the schematic diagram of the frame of grabbing device in accordance with a preferred embodiment of the present invention;
Fig. 7-1 is the schematic diagram of the instrument of the extraction biologically active substance of the grabbing device adopting a preferred embodiment of the invention;
Fig. 7-2 is the schematic diagram of grabbing device crawl casing board in accordance with a preferred embodiment of the present invention.
Reference numeral
Grasping mechanism
1 frame
11,12,13,14 frame frames
15 outside frameworks
16 compressing members
17 base plates
2 compression bars
21 first compression bars
211 first holes
212 second holes
213,214 first compression bar inclined-planes
215,216 axis holes
22 second compression bars
221 the 3rd holes
222 the 4th holes
225,226 axis holes
23 the 3rd compression bars
235,236 axis holes
233,234 the 3rd compression bar inclined-planes
3 tensioning blocks
Reel 32 lower shaft disk on 31
33 axle bed 331332 holes
4 motors
5 thimbles
51 first thimbles
52 second thimbles
53 the 3rd thimbles
54 the 4th thimbles
6 axles
61 first axles
62 second axles
7 bearings
71 head bearings
72 lower bearings
8 guide rails
81 leading rail chairs
82 rear rail seats
9 casing boards
91 the 5th holes
92 the 6th holes
93 seven apertures in the human heads
94 octals
95,96,97,98 casing board frames
99 sleeve pipes
1001 bar magnet methods extract the instrument of biologically active substance
1002 needles
1003 connecting rods
Embodiment
For a better understanding and interpretation of the present invention, the present invention is described in further detail below with reference to accompanying drawings.
Fig. 1-1 is the first direction stereographic map of a kind of grabbing device according to a preferred embodiment of the present invention, and Fig. 1-2 is the exploded perspective view of the second direction of a kind of grabbing device according to a preferred embodiment of the present invention.As shown in Fig. 7-1, the view directions of Fig. 1-2 is the working order direction of grabbing device of the present invention.Above-mentioned grabbing device can be used for adopting bar magnet method to extract the instrument of biologically active substance.In the instrument of said extracted biologically active substance, grabbing device of the present invention can be used for capturing the casing board used in bar magnet method.As shown in Figure 5, casing board has tetragonal dull and stereotyped seat usually, and the sleeve pipe 99 of the some amount arranged regularly or releasably in the centre of described dull and stereotyped seat, such as 4x6 or 8x12 sleeve pipe.As shown in Figure 5, the dull and stereotyped seat of casing board 9 has casing board frame (95,96,97,98), in addition, four angles of the dull and stereotyped seat of above-mentioned casing board 9 has the hollow projection (91,92,93,94) or groove that can contact for grasping mechanism and fix.
As shown in Fig. 1-1 and Fig. 1-2, above-mentioned grabbing device of the present invention comprises frame 1 and grasping mechanism, described grasping mechanism comprises four thimble 5(51,52,53,54), compacting part (comprising the first compression bar 21 and the second compression bar 22) and compacting part drive unit (comprising tensioning block 3, motor 4, guide rail 8 and rolling bearing 7).
The grasping mechanism of above-mentioned grabbing device of the present invention can be arranged in frame, and frame is to the grasping mechanism effect of providing support.In addition, described frame 1 frame of the same or analogous shape and size of frame of casing board that has and need capture.In diagram the preferred embodiments of the present invention, frame 1 has 4 the frame frames (11,12,13,14) corresponding to the casing board square frame (95,96,97,98) that need capture.In addition, in a preferred embodiment of the invention, described frame is set to, at thimble 5(51,52,53,54) projection (91 of casing board is inserted, 92,93,94) time to capture with movable sleeving tube sheet, the surface of frame frame (wherein two, three or four) and the bezel contact of casing board, thus the dull and stereotyped seat of clamping sleeve tube sheet in vertical direction, make dull and stereotyped seat maintenance level, when avoiding casing board to be lifted, produce deformation.
The frame 1 of above-mentioned grabbing device of the present invention also can have the extra compressing member 16 for clamping sleeve tube sheet in vertical direction.In preferred embodiment as shown in Figure 6, there is in the central authorities of frame 1 compressing member 16 of surface (i.e. the lower surface of frame shown in Fig. 6) level of the frame of its surface and frame 1, at thimble 5(51,52,53,54) insert or leave casing board protruding time, its surface (i.e. the lower surface of frame shown in Fig. 6) contacts with the plate face of casing board, the dull and stereotyped seat of clamping sleeve tube sheet in vertical direction further can be played, when avoiding casing board to be lifted, produce the effect of deformation.Described compressing member can be cruciform as shown in the figure or other shape.Described compressing member by with frame monoblock casting, or to be fixed in frame 1 by welding or the mode such as screw thread.Described compressing member avoids the vital role played when producing deformation very when ensureing that casing board is captured by grabbing device of the present invention and lifts.
The frame 1 of above-mentioned grabbing device of the present invention also can have the parts being connected with the cannula translating mechanism adopting bar magnet method to extract the instrument of biologically active substance and fixing, if connecting rod 1003(is see Fig. 7-1), grabbing device can move in vertical and horizontal direction as required thus, makes sleeve pipe be movable to specified location and moves up and down or vibrate.
Above-mentioned grabbing device of the present invention has multiple thimble and compacting part.Compacting part comprises the first compression bar 21 and the second compression bar 22, and described multiple thimble is arranged on the first compression bar 21 and the second compression bar 22 respectively.Described thimble is the hard parts having anchorage force, and hollow projection on four angles can inserting the dull and stereotyped seat of casing board thus also provides enough holding powers, plays promptly and permanent sleeve tube sheet, and the effect of tensioning flat board seat.The material of described thimble can be metal, such as iron or stainless steel, aluminum products as aluminium alloy.The material of described thimble also can be nonmetallic, as hard plastics etc.
In preferred embodiment as shown in the figure, above-mentioned grabbing device there are four thimble 5(51,52,53,54).First compression bar 21 and the second compression bar 22 are arranged on the outside of the frame 12 and 14 of square framework 1 respectively.Thimble 51 and 52 is arranged on the first compression bar 21, and thimble 53 and 54 is arranged on the second compression bar 22.Four thimble 5(51,52,53,54) position corresponds respectively to overhead, aforementioned casing board four angles cardiac prominence and rises (91,92,93,94).Compression bar 21 and 22 is under the driving of compacting part drive unit, can occur relatively near or away from motion (namely separated from one another or close up), thus drive thimble 51,52,53,54 synchronously insert respectively or leave the projection 91 of casing board, 92,93,94, play the effect compressing or unclamp casing board in the horizontal direction, thus realize capturing or decontroling casing board.
Also have for holding and the outside framework (15) of compacting part drive unit of grabbing device as described in fixing in the frame 1 as shown in Fig. 1-1 and 1-2.In one aspect of the invention, described frame can also have the base plate (17) of cover framework or outside framework.Base plate can help the compacting part drive unit realizing described grabbing device to be in closed environment, avoids the impact of pollution or other adverse environmental factors.
As shown in Figure 1-2, the grasping mechanism of device of the present invention comprises compression bar, compression bar is under the driving of compacting part drive unit, can on occurred level direction relative near or away from move, thus drive the multiple thimbles being positioned at frame two ends occur relative near or away from move, leave or insert the projection of casing board respectively, play the effect capturing casing board.
Illustrating in device of the present invention, comprise 3 compression bars, is the first compression bar 21, second compression bar 22 and the 3rd compression bar 23 respectively.
The structural representation of above-mentioned first compression bar 21, above-mentioned second compression bar 22 and above-mentioned 3rd compression bar 23 is respectively as shown in Fig. 2-1, Fig. 2-2 and Fig. 2-3.Above-mentioned first compression bar 21 comprises the first hole 211 and the second hole 212 for fixing thimble, and above-mentioned second compression bar 22 comprises the 3rd hole 221 for fixing thimble and the 4th hole 222.Fig. 2-4 is the wiring layout of above-mentioned second compression bar 22, above-mentioned 3rd compression bar 23 and axle 6.
In addition, as shown in Fig. 1-2, Fig. 2-1, Fig. 2-3 and Fig. 3, the middle part of above-mentioned first compression bar 21 and above-mentioned 3rd compression bar 23 has the first compression bar inclined-plane (213,214) and the 3rd compression bar inclined-plane (233,234).As hereafter described in detail, described inclined-plane be arranged so that compacting part drive unit of the present invention can simply with reliably realize the first compression bar 21 and the 3rd compression bar 23 and relative (oppositely) occur be synchronized with the movement.
As shown in Figure 1-2, the first compression bar 21 and the second compression bar 22 are arranged on the square frame two ends of framework 1 respectively.In preferred illustrated embodiment, the first compression bar 21 and the second compression bar 22 are arranged on the outside of frame 11 and 13 respectively, and are fixed on framework 1 by axle 6 slip.Illustrate in device of the present invention, above-mentioned axle 6 comprises 2 axles, i.e. the first axle 61 and the second axle 62, and it is each passed through on the frame 11 and 13 of framework 1, is fixed on thus on the framework of grabbing device of the present invention.
As shown in Figure 2, the first compression bar 21 has axis hole 215 and 216, first axle 61 that can pass for the first axle 61 and the second axle 62 and the second axle 62 can be each passed through axis hole 215 and 216, and the first compression bar 21 is slidably connected in above-mentioned crawl frame 1 thus.
First compression bar 21 and the 3rd compression bar 23 are arranged on the same side of the frame of framework 1.Namely the second compression bar 22 and the 3rd compression bar 22 are arranged on the frame two ends of framework 1.Second compression bar 22 and the 3rd compression bar 23 have the axis hole 225 and 226 passed for the first axle 61 and the second axle 62 respectively, and axis hole 235 and 236.First axle 61 is through axis hole 225 and 235, and above-mentioned second axle 62 is through axis hole 226 and 236.3rd compression bar 23 and the second compression bar 22 are fixedly connected with the second axle 62 with the first axle 61 by screw thread or the mode such as to weld.By connection and the gearing of axle 6, the 3rd compression bar 23 and the second compression bar 22 synchronizing moving in the same way.
Grabbing device of the present invention be arranged so that the first compression bar 21 and the second compression bar 22 in the horizontal direction reverse sync move, realize thus the first compression bar 21 and the second compression bar 22 occur relative away from or close motion, thus drive the thimble laying respectively at the first compression bar 21 and the second compression bar 22 occur relative away from or close motion, play the effect of crawl casing board.
As shown in Fig. 1-1 and Fig. 1-2, in one aspect of the invention, the first compression bar 21 and the 3rd compression bar 23 are arranged on the same side of the frame of framework 1, up and down in the stacked outside framework 15 being arranged on above-mentioned frame 1.3rd compression bar 23 is fixedly connected with by screw thread or the mode such as to weld by axle 6 and the second compression bar 22.By connection and the gearing of axle 6, the 3rd compression bar 23 and the second compression bar 22 synchronizing moving in the same way.Following mask body is set forth, apparatus of the present invention be arranged so that the reverse sync of the first compression bar 21 and the 3rd compression bar 23 moves, the reverse sync realizing the first compression bar 21 and the second compression bar 22 thus moves.
The compacting part drive unit of the grasping mechanism of apparatus of the present invention can drive above-mentioned compression bar occur relatively away from or close motion, thus drive the thimble laying respectively at the first compression bar 21 and the second compression bar 22 occur relative away from or close motion, play the effect of crawl casing board.
The compacting part drive unit of diagram the preferred embodiments of the present invention device comprises tensioning block 3, motor 4, guide rail 8 and bearing 7(head bearing 71 and lower bearing 72).
The structural representation of tensioning block 3 as shown in Figure 3.Above-mentioned tensioning block 3 comprises columniform upper reel 31 and the lower shaft disk 32 at axle bed 33 and axle bed two ends.Described head bearing 71 and lower bearing 72 are set in outside reel 31 and lower shaft disk 32.The motor shaft that axle bed 33 has power supply machine 4 passes and fixing hole 331.Motor shaft is fixed with tensioning block 3 in hole 331 by axle sleeve, realizes the driving to tensioning block thus.As shown in Fig. 1-1 and 1-2, upper reel 31 and lower shaft disk 32 can come in contact respectively by head bearing 71 and the inclined-plane (213,214) of lower bearing 72 and the first compression bar 21 and the inclined-plane (233,234) of the 3rd compression bar 23.Thus, when motor 4 drives tensioning block 3 to seesaw, by head bearing 71 and lower bearing 72 respectively by described inclined-plane (213,214) or inclined-plane (233,234) contact and extrude the first compression bar 21 or the 3rd compression bar 23, promote the first compression bar 21 and the 3rd compression bar 23 thus relative (oppositely) occur respectively to be synchronized with the movement, thus drive lay respectively at the first compression bar 21 and the thimble of the second compression bar 22 occur relative away from or close motion, play the effect of crawl casing board.
In one aspect of the invention, described head bearing 71 and lower bearing 72 are rolling bearing.Employing rolling bearing can more effectively on the inclined-plane (213 by the first compression bar 21,214) inclined-plane (233,234) of the and three compression bar 23 come in contact with extrude promote the first compression bar 21 and the 3rd compression bar 23 occur respectively relative (oppositely) be synchronized with the movement time more effectively avoid clamping stagnation occurs.
In one aspect of the invention, the compacting part drive unit of device of the present invention also comprises guide rail 8.The axle bed 33 of tensioning block 3 has the hole 332 passed for described guide rail and fix.Of the present invention in one of them, the compacting part drive unit of device comprises point two circular guides being located at the motor shaft both sides of motor 4.The two ends of guide rail 8 are erected on leading rail chair 81 and rear rail seat 82.Leading rail chair 81 and rear rail seat 82 are fixed on the base plate 17 of frame outside framework by modes such as welding or screw threads.Tensioning block 3 passing hole 332 and guide rail 8 are slidably connected.Tensioning block 3 can under the driving of motor 4, movable in the scope of leading rail chair 81 and rear rail seat 82, and it is foregoing, by the inclined-plane (213 of head bearing 71 and lower bearing 72 and the first compression bar 21,214) inclined-plane (233 of the and three compression bar 23,234) come in contact and extrude to promote the first compression bar 21 and the 3rd compression bar 23 reverse sync in the horizontal direction moves, namely the reverse sync of the first compression bar 21 and the second compression bar 22 moves.By dividing two circular guides being located at motor shaft both sides, can fixed electrical machinery be assisted, tensioning block 3 and bearing 7, and effectively preventing tensioning block 3 from bearing 7, rotating around axle mirror image relative to motor shaft occurring.
As shown in Figure 4, in a preferred embodiment of the invention, the thimble 5 of grabbing device of the present invention has pyramid type top and columniform bottom.Described thimble is releasably assemblied on compression bar by columniform bottom.In addition, described thimble inserts the hollow projection of casing board by top (such as cylindrical or pyramid type).The thimble of grabbing device of the present invention inserts the hollow projection of casing board or the top of groove (91,92,93,94) can be designed to circular cone build.Thus, thimble, when inserting casing board hollow protruding, expands gradually with the contact surface of this hollow projection or groove, plays tensioning and more firmly and the effect fixed of this hollow projection or groove.This design greatly increases grabbing device of the present invention at the firmness capturing casing board.
The mode of operation of grabbing device of the present invention or principle of work:
Grabbing device of the present invention is used for extracting in the instrument of biologically active substance in bar magnet method capturing casing board.In the bar magnet method extracting biologically active substance, need magnetic sleeve pipe to immerse sample (normally liquid), make the particle that can be attracted magnetically in mixture concentrate on sleeve surface by the action of a magnetic field.Sleeve pipe is the pipe with closed bottom and hollow tube chamber, can have such as elongated cylinder form.In the method, make sleeve pipe produce magnetic by inserting bar magnet, attract the granular absorption that can be attracted magnetically in mixture to sleeve surface, when bar magnet is detached, sleeve pipe loses magnetism, and the particle be attracted magnetically departs from sleeve pipe.Extract in the instrument of biologically active substance in bar magnet method, sleeve pipe needs to move up and down, and can insert the setting container holding sample thereunder thus, thus realizes the absorption of biologically active substance, rinsing and purifying.
Bar magnet method is extracted in the instrument of biologically active substance or method, and described sleeve pipe can provide with the form of casing board.As shown in Figure 5, the dull and stereotyped seat of casing board has tetragonal frame, and four angles of its dull and stereotyped seat have can for the hollow projection of grasping mechanism contact.
Fig. 7-1 and Fig. 7-2 is that grabbing device of the present invention extracts the work and the structural representation that capture casing board in the instrument of biologically active substance in bar magnet method.
As shown in Fig. 7-1, the instrument 1001 of described extraction biologically active substance has needle 1002 and controls the mechanism that needle moves up and down.Grabbing device 1 of the present invention can capture and permanent sleeve tube sheet 9, thus realizes the crawl of the sleeve pipe 99 be arranged on casing board and fixing.Grabbing device 1 is connected with the mechanism controlling grabbing device horizontal or vertical movement by connecting rod 1003, realizes sleeve pipe to the location of specified location and move up and down or vibrate.
(Fig. 7-1 and Fig. 7-2 is the schematic diagram at contrary visual angle) shown in Fig. 7-2 is the working order of grabbing device crawl casing board of the present invention.The thimble 51,52,53 and 54 of grabbing device of the present invention inserts in four holes of casing board 9 respectively, thus simultaneously, the frame frame of frame 1 can be close to the frame of casing board, thus assist not produce deformation when above-mentioned casing board 9 is lifted, also there is not relative displacement in the sleeve pipe in above-mentioned casing board 9.
When above-mentioned grabbing device needs to capture above-mentioned casing board 9, motor 4 moves forward and backward by driving tensioning block 3, respectively by described inclined-plane (213,214) or inclined-plane (233,234) contact and extrude the first compression bar 21 or the 3rd compression bar 23, make above-mentioned first compression bar 21 and with synchronization-moving second compression bar 22 relative separation of the 3rd compression bar 23 or close up, make the 3rd thimble 53 in the first thimble 51 in above-mentioned first compression bar 21 and the second thimble 52 and the second compression bar 22 and the 4th thimble 54 leave respectively or insert in four holes of casing board 9.
Above-mentioned grabbing device and the self-reacting device comprising above-mentioned grabbing device are a kind of preferred embodiment of the present invention, all fall within the scope of protection of the present invention identical with it of structure and function.The thimble that such as can set different number according to the shape of casing board and size fixes casing board, and such as, four thimbles in the preferred embodiment of the present invention also can replace to two thimbles, captures casing board from both sides.Thimble can also replace to other hard gripper components having anchorage force, such as identical compared with butt diameter size with thimble centrum or right cylinder, and material is partially hard anchorage force, such as irony product and aluminium alloy.
Number and the length size of compression bar also can adjust.Such as compression bar also can replace with two by three of the preferred embodiment of the present invention, and reserved category is like the first compression bar in the preferred embodiment of the present invention and the second compression bar; Compression bar power set can Direct driver first compression bar and the second compression bar, makes to be arranged in the first compression bar and the thimble of the second compression bar produces displacement, occurs to be separated or to close up.
Although describe in detail about example embodiment of the present invention and advantage thereof, being to be understood that when not departing from the protection domain of spirit of the present invention and claims restriction, various change, substitutions and modifications can being carried out to these embodiments.For other examples, those of ordinary skill in the art should easy understand maintenance scope in while, the order of processing step can change.
In addition, range of application of the present invention is not limited to the technique of the specific embodiment described in specification sheets, mechanism, manufacture, means, method and step.From disclosure of the present invention, to easily understand as those of ordinary skill in the art, for the technique existed at present or be about to develop, mechanism, manufacture, means, method or step later, wherein their perform the identical function of the corresponding embodiment cardinal principle that describes with the present invention or obtain the identical result of cardinal principle, can apply according to the present invention to them.Therefore, claims of the present invention are intended to these technique, mechanism, manufacture, means, method or step to be included in its protection domain.
Claims (11)
1. one kind is extracted the grabbing device capturing casing board in the instrument of biologically active substance for bar magnet method, it is characterized in that described grabbing device comprises frame (1) and grasping mechanism, wherein said frame (1) has square frame, described grasping mechanism comprises multiple thimble (5), compacting part (2) and compacting part drive unit, described compacting part (2) comprises outside the relative frame of two of being installed on described frame (1), the first compression bar (21) be arranged in parallel and the second compression bar (22), described multiple thimble (5) is arranged on the first compression bar (21) and the second compression bar (22) respectively,
Wherein, described first compression bar (21) and the second compression bar (22) are under the driving of compacting part drive unit, can move the synchronous backward in occurred level direction, thus drive the described multiple thimbles be fixed thereon occur synchronous near or away from movement, can leave or insert the projection on casing board four angles thus respectively
Wherein, described multiple thimble is four thimbles (51, 52, 53, 54), thimble (51) and (52) are arranged on the first compression bar (21), thimble (53) and (54) are arranged on the second compression bar (22), four thimbles (51, 52, 53, 54) position corresponds respectively to the projection on casing board four angles, compression bar (21) and (22) are under the driving of compacting part drive unit, can occur relative to away from or the moving of close motion, thus drive thimble (51, 52, 53, 54) synchronously insert respectively or leave the projection on casing board four angles, compress in the horizontal direction or unclamp casing board,
Wherein, described compacting part (2) also comprises the 3rd compression bar (23), second compression bar (22) is fixedly connected with by axle (6) the 3rd compression bar (23), by connection and the gearing of axle (6), 3rd compression bar (23) and the second compression bar (22) synchronizing moving in the same way
Wherein, first compression bar (21) and the second compression bar (22) be arranged in parallel, be installed on outside the relative frame of two of described frame (1) respectively, first compression bar (21) and the 3rd compression bar (23) are arranged on the frame homonymy of framework (1), first compression bar (21) and the 3rd compression bar (23) can do synchronous backward and move under the driving of compacting part drive unit, first compression bar (21) and the second compression bar (22) do reverse sync and move thus, the middle part of the first compression bar (21) and the 3rd compression bar (23) has the first compression bar inclined-plane (213 respectively, 214) the and three compression bar inclined-plane (233, 234),
Wherein, described compacting part drive unit comprises tensioning block (3), motor (4) and bearing (7), described tensioning block (3) comprises upper reel (31) and the lower shaft disk (32) at axle bed (33) and axle bed two ends, head bearing (71) and lower bearing (72) are set in described upper reel (31) and lower shaft disk (32) outside, the motor shaft that described axle bed (33) has power supply machine (4) passes and fixing hole (331)
Wherein, upper reel (31) and lower shaft disk (32) can respectively by head bearing (71) and lower bearing (72) inclined-planes (213 with the first compression bar (21), 214) inclined-plane (233 of the and three compression bar (23), 234) come in contact, thus, when motor (4) drives tensioning block (3) to seesaw, the upper reel (31) of tensioning block (3) and lower shaft disk (32) extrude the first compression bar inclined-plane (213 respectively by head bearing (71) and lower bearing (72), 214) the and three compression bar inclined-plane (233, 234), promote the first compression bar (21) and the motion of the 3rd compression bar (23) generation synchronous backward thus, thus drive the thimble generation laying respectively at the first compression bar (21) and the second compression bar (22) relative away from or close motion.
2. grabbing device according to claim 1, is characterized in that, described head bearing (71) and lower bearing (72) are rolling bearing.
3. grabbing device according to claim 1, it is characterized in that, described compacting part drive unit also comprises point two guide rails (8) being located at the motor shaft both sides of motor (4), and tensioning block (3) is slidably connected with guide rail (8) through guide rail (8) by the hole (332) in its axle bed (33).
4. grabbing device according to claim 1, it is characterized in that, described frame (1) has outside framework (15), and the first compression bar (21) and the 3rd compression bar (23) of described compacting part drive unit and compacting part (2) are arranged in described outside framework (15).
5. grabbing device according to claim 1, it is characterized in that, the frame frame of described frame (1) is set to, when the thimble (5) of grasping mechanism inserts or leaves casing board protruding, the surface of frame frame of described frame (1) and the bezel contact of casing board the dull and stereotyped seat of clamping sleeve tube sheet.
6. grabbing device according to claim 5, it is characterized in that, described frame (1) also has the compressing member (16) for clamping sleeve tube sheet in vertical direction, the surface level of the surface of described compressing member (16) and the frame of frame (1), be set to when thimble (5) inserts or leave casing board protruding, its surface contacts dull and stereotyped seat of also clamping sleeve tube sheet with the plate face of casing board.
7. grabbing device according to claim 1, is characterized in that, described thimble is hard parts.
8. grabbing device according to claim 1, is characterized in that, described thimble has pyramid type top.
9. extract an instrument for biologically active substance for bar magnet method, it is characterized in that, described instrument comprises the grabbing device according to any one of claim 1-8.
10. instrument according to claim 9, it is the instrument for Isolation and purification nucleic acid.
11. 1 kinds use the grabbing device according to any one of claim 1-8 or the instrument described in claim 9 or 10 to capture in the technique of bar magnet method extraction biologically active substance and handle the method for the casing board of carrying sleeve pipe.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310371016.9A CN103805498B (en) | 2013-08-23 | 2013-08-23 | Grabbing device and there is the instrument of extraction biologically active substance of this grabbing device |
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| CN201310371016.9A CN103805498B (en) | 2013-08-23 | 2013-08-23 | Grabbing device and there is the instrument of extraction biologically active substance of this grabbing device |
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| CN103805498B true CN103805498B (en) | 2015-11-25 |
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| US6447729B1 (en) * | 1993-02-01 | 2002-09-10 | Labsystems Oy | Method and means for magnetic particle specific binding assay |
| CN102172910A (en) * | 2011-01-26 | 2011-09-07 | 上海铭源数康生物芯片有限公司 | Device for fetching and releasing magnetic sleeve |
| WO2012057548A2 (en) * | 2010-10-27 | 2012-05-03 | Bioneer Corporation | Automatic real-time pcr system for the various analysis of biological smaple |
| CN203513663U (en) * | 2013-08-23 | 2014-04-02 | 北京金麦格生物技术有限公司 | Grasping device used in instrument for extracting biologic active substance by using magnetic bar method |
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2013
- 2013-08-23 CN CN201310371016.9A patent/CN103805498B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6447729B1 (en) * | 1993-02-01 | 2002-09-10 | Labsystems Oy | Method and means for magnetic particle specific binding assay |
| WO2012057548A2 (en) * | 2010-10-27 | 2012-05-03 | Bioneer Corporation | Automatic real-time pcr system for the various analysis of biological smaple |
| CN102172910A (en) * | 2011-01-26 | 2011-09-07 | 上海铭源数康生物芯片有限公司 | Device for fetching and releasing magnetic sleeve |
| CN203513663U (en) * | 2013-08-23 | 2014-04-02 | 北京金麦格生物技术有限公司 | Grasping device used in instrument for extracting biologic active substance by using magnetic bar method |
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| CN103805498A (en) | 2014-05-21 |
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Address after: Wujin District of Jiangsu city of Changzhou province Changzhou 213000 West Road No. 9, Taihu science and technology industry director B1 Applicant after: CHANGZHOU GENMAG BIOTECHNOLOGY CO., LTD. Address before: 100176 No. 3 north Yongchang Road, Daxing District economic and Technological Development Zone, Beijing, 707 Applicant before: Beijing Genmagbio Biotechnology Co., Ltd. |
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