CN103800670A - Traditional Chinese medicinal preparation for treating kidney-yang deficiency and preparation method thereof - Google Patents

Abstract

The invention discloses a traditional Chinese medicinal preparation for treating kidney-yang deficiency. The traditional Chinese medicinal preparation is prepared from the following bulk drugs in parts by weight: 10-20 parts of epimedium, 10-20 parts of fructus rosae laevigatae, 10-20 parts of rhizoma cibotii, 10-20 parts of glossy privet fruit, 10-20 parts of placenta hominis, 10-20 parts of prepared rehmannia, 10-20 parts of herba taxilli, 10-20 parts of dodder, 10-20 parts of plantain seed, 10-20 parts of prepared fleece-flower root, 10-20 parts of polygonatum kingianum, 10-20 parts of codonopsis pilosula, 10-20 parts of poria, 10-20 parts of white peony root, 10-20 parts of schisandra chinensis, 10-20 parts of raspberry, and 10-20 parts of dogwood. The traditional Chinese medicinal preparation is prepared by selecting traditional Chinese medicinal materials with the effects of tonifying kidney and yang, nourishing essence and blood, and strengthening muscles and bones, wherein most traditional Chinese medicinal materials taste light and remarkable in drug effect, so that the traditional Chinese medicinal preparation can be taken for a long time, can be easily accepted by people to achieve the effects of nourishing yin and tonifying kidney, strengthening muscles and bones, and tonifying yang-qi, and is remarkable in effects for treating extreme chilliness, dizziness, listlessness, pale or dark complexion, impotence and premature ejaculation of males, infertility due to uterus coldness of females, and other kidney-yang deficiency symptoms.

Description

A kind of Chinese medicine preparation for the treatment of insufficiency of kidney-YANG and preparation method thereof

Technical field

The present invention relates to contain the medical pharmacy that derives from plant, animal or raw mineral materials, particularly a kind of Chinese medicine preparation for the treatment of insufficiency of kidney-YANG and preparation method thereof.

Background technology

Modern medicine thinks that insufficiency of kidney-YANG is relevant with neuroendocrine-immune system (NEIS).Deficiency of the kidney yin is the syndrome of the cloudy gas of kidney, YIN-fluid being insufficient performance.How to be impairment of the kidney by prolonged illness, or innate deficiency sexual strain, or cross due to the product that take warm-dryness syndrome misfortune the moon.The treatment of deficiency of the kidney yin comprises Drug therapy and auxiliary treatment.No matter be the deficiency of YIN or yang deficiency might as well, scarcity to a certain extent, be exactly deficiency of YIN affecting YANG, or deficiency of YANG affecting YIN, for example, the insufficiency of kidney-YANG time grown the symptom that has deficiency of the kidney yin, two kinds of symptoms have much exactly.

Insufficiency of kidney-YANG, i.e. kidney deficient and weak YANG QI, is the disease of kidney yang-energy exhaustion performance.How by innate deficiency of YANG in the body, or old deficiency of the kidney, or prolonged illness is impairment of the kidney, and the factor such as excess of sexual intercourse causes.Clinical manifestation is soreness of waist and knee joint, aversion to cold and cold limbs, especially with lower limb for very, the vertigo, lethargy, pale complexion; Or dark, the fat tongue of light red tongue is white, deep and weak pulse; Sexual impotence, premature ejaculation, women's infertility, or stool chronic diarrhea is more than, aqueous-grainy diarrhea, Diarrhoea; Or edema, waist is below for very, by depression do not rise, very abdominal part distending pain, cardiopalmus cough with asthma.So when kidney-replenishing, must tonifying kidney-yin, and want treating YANG within YIN, and neither be simply in tonifying kidney-yin only with the medicine of tonifying kidney-yin.

Kidney is hidden first acquired essence, and kidney essense turns to kidney qi, wherein to body have warm, excite, excited, evaporate, seal and hide and restrict the actor such as YIN-cold and be referred to as kidney yang, be also called Yuanyang, kidney-Yang, kidney-yang; To body have moist, quiet, being shaped and suppressing excessively the actor such as sun heat is referred to as kidney yin, is also called Kidney-Yin, Kidney-Yin, true water.Kidney yang can promote that the metabolism of human body is gasification, promotes the change of essence and blood body fluid give birth to and make it to be converted into energy, and the process of the various physiological activities of human body is accelerated, and heat production increases, in high spirits; Kidney yin suppresses or the excessive metabolic processes of reducing human, making essence and blood body fluid be converted into energy reduces, the process of the various physiological activities of human body slows down, heat production reduces relatively, and make gas be polymerized to shape and be essence and blood body fluid, spirit is also tending towards keeping the two in quiet opposes each other and yet also complement each other, and common adjusting controlled the function activity of human body and the metabolic process of essence and blood body fluid.

According to the yin-yang theory of Chinese medicine, negative and positive have limitlessly detachable, and kidney is hidden first acquired essence, and it is cloudy that essence belongs to, so claim cloudy essence, QI belonging to YANG, so claim yang-energy, is collectively referred to as cloudy the vivid YANG QI.Kidney essense belongs to cloudy essence, and forked rubbing more again among cloudy essence, wherein can be divided into again cloudy smart the yang aspect of yang essence and cloudy smart the yin aspect of yin essence.When cloudy smart the yang aspect of yang damage of essence is empty, there are soreness of waist and knee joint, the spinal column Symptoms such as sour tired out, the fatiguability of weak, bone that wither, the Symptoms such as cloudy smart the yin aspect of yin damage of essence has soreness of the waist and knees, talalgia, dry mouth and tougue when empty, quenches one's thirst, insomnia and dreamful sleep.Cloudy smart the yang aspect of yang damage of essence is how in close relations with insufficiency of kidney-YANG when empty, when cloudy smart the yin aspect of yin damage of essence is empty how and deficiency of the kidney yin in close relations.So when insufficiency of kidney-YANG except there being fear of cold to be often afraid of cold etc. performance with soreness of waist and knee joint, the spinal column symptoms such as sour tired out, the fatiguability of weak, bone of withering, when deficiency of the kidney yin except having the performances such as the deficiency of YIN is scorching often with soreness of the waist and knees, talalgia, dry mouth and tougue, the symptom such as quench one's thirst.

From also finding out that insufficiency of kidney-YANG is larger than deficiency of the kidney yin on people's impact here, positive main the moon is from, interdependence between yin and yang in human body.Thyroxin, glucocorticoid, androgen, calcitonin, dimension D3 etc. belong to the category of sun essence (cloudy smart the yang aspect of yang essence).Estrogen, melatonin, insulin etc. mostly belong to the category of cloudy essence (cloudy smart the yin aspect of yin essence).Divide from material and function, it is cloudy that material belongs to, and function belongs to sun, all functional activities of human body all belong to yang-energy, all constitutes of human body all belong to cloudy essence, but in human body, do not have a kind of gas of immaterial pure function, and in Chinese medicine, gas is the cereal nutrient that a kind of vigor is very strong.Therefore gas is the dialectical unity of material and function.Kidney qi is given birth to by kidney essense, and broad sense kidney qi refers to the functional activity of kidney, has comprised kidney yin, kidney yang.Narrow sense kidney qi refers to the part that plays astringency inducing, the effect of envelope Tibetan in kidney, and instability of kidney QI generally, refers to narrow sense kidney qi.Kidney yin is a part for renal function activity, play make moist, Thyme Extract.The implication of usual said kidney yin has also comprised kidney essense in fact interior, sometimes specially refers to kidney essense by kidney yin.Therefore the implication of negative and positive is different in different linguistic context.Sometimes represent all functions activity of human body with yang-energy, sometimes singly refer in bodily fuctions's activity and play part warm, impetus, therefore will note differentiating.Negative and positive can refer to bring into play in qi of zang-fu viscera the gas of adverse effect, and also can make a comment or criticism gas and pathogen also can refer to clearing heat in QI system and foul smell.Cloudy gas does not always represent bad, disadvantageously in a word, and yang-energy is always not good yet.Mention cloudy gas and always do not think pathogenic QI in the body, the pathogen of the human body just referring to, it can also refer to a requisite part in human body physiological function activity.In the traditional Chinese medical science, sometimes refer to QI of five ZANG-organs with cloudy gas

Generally, it is aching and limp that insufficiency of kidney-YANG shows as chills and pain of the waist and kness more in symptom, cold extremities with aversion to cold, and especially waist is cool to issue, always wear two clothes, nocturia, neurolysis, sexual impotence than others at ordinary times more, edema, clear urine in large amounts, incontinence or unfavorable, the symptom of the yang deficiency endogenous cold such as pale tongue.It is pointed out that on the one hand various suffering from a deficiency of the kidney all centered by kidney-jing deficiency, so the performance of deficiency of kidney-essence is among various suffering from a deficiency of the kidney; On the other hand, owing to being subject to the impact of various factors, specifically everyone performance again take wherein one or more as cardinal symptom." suffering from a deficiency of the kidney " of often saying on present society, as seminal emission, sexual impotence, premature ejaculation, hyposexuality, sterile, infertile etc., is actually suffering from a deficiency of the kidney of narrow sense.Sensu lato suffering from a deficiency of the kidney comprises a series of General Symptoms.Chinese traditional treatment is a dialectical process, and a lot of people do not understand it oneself is to belong to what symptom, abuse simply tonic, and result can only be controlled poorer and poorer.

Insufficiency of kidney-YANG symptom is utilized modern instrument to detect and is often come to nothing, and doctor trained in Western medicine also can only, from Subhealth treating, also be felt simply helpless to its treatment.Chinese medicine exists dual regulation, when central concentration is too high, may causes certain antagonism, and make drug effect influenced; When low dosage, possible drug level is too low, causes drug effect not aobvious.And for the traditional Chinese medical science, all there is no larger breakthrough no matter be from the formula components various aspects of traditional Chinese medical science mechanism, dosage form or the medicine of institute's foundation, take mouthfeel not good, medicine poverty, take very inconvenient, various patients' demand can not be met, the city life of modern high rhythm can not be met.In the face of more and more ubiquitous insufficiency of kidney-YANG states of urbanite, Chinese Herbs is slow, takes trouble, and the traditional therapy of unsuitable long-term taking does not improve, and also ideal not necessarily of effect, can not accepted extensively by masses.And Chinese medicine preparation just in time whereby opportunity greatly develop, meet people's building body, the happy hope of living.

Summary of the invention

Technical problem to be solved by this invention is, as insufficiency of kidney-YANG cannot definitely be diagnosed and treat to doctor trained in Western medicine, can only regulating sub-health of human body state be main, and the cycle is long, and expense is large, and uncertain therapeutic efficacy is cut, and there is no definite therapeutic scheme, is also difficult for adhering to; Traditional Chinese medical science traditional therapy is also nothing more than symptomatic treatment in acute condition, the rules such as relieving the primary symptom in a chronic case, and that tests is clinical, though there is certain curative effect, produces effects slowly, and effective percentage is low; And it is not good to take mouthfeel, medicine poverty, very inconvenient.Chinese medicine exists dual regulation, when central concentration is too high, may causes certain antagonism, and make drug effect influenced; When low dosage, possible drug level is too low, causes drug effect not aobvious.In the face of more and more ubiquitous insufficiency of kidney-YANG states of urbanite, Chinese Herbs is slow, takes trouble, and the traditional therapy of unsuitable long-term taking does not improve, and also ideal not necessarily of effect, can not accepted extensively by masses.

For solving the problems of the technologies described above, the invention provides a kind of Chinese medicine preparation for the treatment of insufficiency of kidney-YANG, the weight fraction ratio of described Chinese medicine preparation crude drug is: 10～20 parts of Herba Epimedii, 10～20 parts of Fructus Rosae Laevigatae, 10～20 parts of Rhizoma Cibotii, 10～20 parts of Fructus Ligustri Lucidi, 10～20 parts of Placenta Hominiss, 10～20 parts, Radix Rehmanniae Preparata, 10～20 parts of Herba Taxillis, 10～20 parts of Semen Cuscutae, 10～20 parts of Semen Plantaginiss, 10～20 parts of Radix Polygoni Multiflori Preparata, 10～20 parts of Rhizoma Polygonatis, 10～20 parts of Radix Codonopsis, 10～20 parts, Poria, 10～20 parts of the Radix Paeoniae Albas, 10～20 parts of Fructus Schisandrae Chinensis, 10～20 parts of Fructus Rubies, 10～20 parts of Fructus Corni.

The Chinese medicine preparation of described treatment insufficiency of kidney-YANG, described in it, the weight fraction of Chinese medicine preparation crude drug ratio can be: 10～15 parts of Herba Epimedii, 10～15 parts of Fructus Rosae Laevigatae, 10～15 parts of Rhizoma Cibotii, 10～15 parts of Fructus Ligustri Lucidi, 10～15 parts of Placenta Hominiss, 10～15 parts, Radix Rehmanniae Preparata, 10～15 parts of Herba Taxillis, 10～15 parts of Semen Cuscutae, 10～15 parts of Semen Plantaginiss, 10～20 parts of Radix Polygoni Multiflori Preparata, 10～20 parts of Rhizoma Drynariae, 10～20 parts of Radix Codonopsis, 10～20 parts, Poria, 10～20 parts of the Radix Paeoniae Albas, 10～20 parts of Fructus Schisandrae Chinensis, 10～20 parts of Fructus Rubies, 10～20 parts of Fructus Corni.

The Chinese medicine preparation of described treatment insufficiency of kidney-YANG, described in it, the weight fraction of Chinese medicine preparation crude drug ratio can also be: 10～20 parts of Herba Epimedii, 10～20 parts of Fructus Rosae Laevigatae, 10～20 parts of Rhizoma Cibotii, 10～20 parts of Fructus Ligustri Lucidi, 10～20 parts of Placenta Hominiss, 10～20 parts, Radix Rehmanniae Preparata, 10～20 parts of Herba Taxillis, 10～20 parts of Semen Cuscutae, 10～20 parts of Semen Plantaginiss, 10～15 parts of Radix Polygoni Multiflori Preparata, 10～15 parts of Rhizoma Polygonatis, 10～15 parts of Radix Codonopsis, 10～15 parts, Poria, 10～15 parts of the Radix Paeoniae Albas, 10～15 parts of Fructus Schisandrae Chinensis, 10～15 parts of Fructus Rubies, 10～15 parts of Fructus Corni.

For solving the problems of the technologies described above, the present invention also provides a kind of preparation method of Chinese medicine preparation for the treatment of insufficiency of kidney-YANG, and described in it, the preparation process of Chinese medicine preparation comprises:

A. described crude drug is put into ethanol heating and refluxing extraction 2 times, as component 1;

B. get the medicinal residues after above-mentioned ethanol extraction, the heating and refluxing extraction that adds water 2 times, as component 2 again;

C. above-mentioned two kinds of extracting solution components 1 and component 2 are merged, after concentrating, add dextrin and be in harmonious proportion, after disinfection, be prepared into oral solutions.

In described step a, in the ethanol that can add 5-10 doubly to measure described crude drug, soak 1-2 hour, heating extraction 2 times, each 1-2 hour, remove supernatant, merge extractive liquid,, 100-120 order filters, then is the ultrafiltration post ultrafiltration of 5000-10000 through the molecular weight that dams, 1.36 extractum when ultrafiltrate concentrating under reduced pressure relative density is 80 ℃, heating is concentrated into paste, and stand for standby use becomes component 1.

In described step b, the medicinal residues of the crude drug of above-mentioned steps ethanol extraction can be put into 10 times of water gagings, heating and refluxing extraction 2 times, each extraction time is 1～2 hour, 100-120 order filters, then is the ultrafiltration post ultrafiltration of 5000-10000 through the molecular weight that dams, 1.36 extractum when ultrafiltrate concentrating under reduced pressure relative density is 80 ℃, heating is concentrated into paste, becomes component 2.

In described step c, after component 1 and component 2 can being merged, insert in economic benefits and social benefits vacuum concentrator the concentrated solution that while being concentrated into 90 ℃, relative density is 1.05, set to 0～5 ℃ of deepfreezes 24 hours; Cold preservation liquid is added to 0.3% filter aid kieselguhr, filter, filtrate is inserted in economic benefits and social benefits vacuum concentrator again, is concentrated into every 1ml containing 1g crude drug amount; Unguentum after concentrated adds dextrin and is in harmonious proportion, and after ultraviolet ray sterilization bactericidal, bottles.

For solving the problems of the technologies described above, the present invention also provides a kind of preparation method of Chinese medicine preparation for the treatment of insufficiency of kidney-YANG, and its preparation process can also be:

A. described crude drug is pulverized, sieved;

B. the granule sieving is soaked, is extracted 3 times;

C. 3 concentrated proper honey of sneaking into of extract are in harmonious proportion, after ultraviolet ray sterilization bactericidal, bottle.

In described step a, can also get crude drug and clean and dry, in 70 ℃ of baking ovens, dry 8-12h, take out and be placed in drying baker coolingly, pulverize 80-100 mesh sieve with pulverizer.

In described step b, the particulate matter that sieves can also be soaked in water 12 hours, then put into extraction pot heated and boiled 1 hour, filter filtrate for later use; Filtering residue adds water, and heating, boils 45 minutes for the second time, filters filtrate for later use; Filtering residue adds water again, heated and boiled half an hour for the third time, filters; Three filtrates are combined, powder is placed in 70-90% ethanol and soaks 1-2 hour, is heated to 60 ℃-70 ℃, lixiviate 2h in water-bath, every 10min stirs once, by filtered through gauze, in residue, add 50-60% ethanol, 60 ℃-70 ℃ are continued lixiviate 2h, every 10min stirs once, filtered through gauze, merges lixiviating solution, is condensed into pasty state.

The present invention is directed to above-mentioned technological deficiency, the Chinese crude drug of some reinforcing the kidney and supporting YANG of having chosen carefully, benefiting essence-blood, bone and muscle strengthening is prepared into oral liquid, and the most mouthfeel of these medical materials is light, and drug effect is remarkable, can long-term taking, make people be easy to accept, can reach building body, enriching yin and nourishing kidney, strengthening bone and muscle, effect of YANG invigorating gas, especially for aversion to cold and cold limbs, the vertigo, lethargy, pale complexion or dark; Impotence premature ejaculation, the insufficiency of kidney-YANG symptom effects such as women's infertility are obvious.

The specific embodiment

" Diagnostics of Chinese Medicine " teaching material is thought: syndrome of deficiency of kidney-YANG, is caused by kidney yang deficiency.Generally see the cold syndrome resembling as dialectical main points with whole body underactivity companion.When insufficiency of kidney-YANG, the promotion of its warm and functional activity to the each internal organs of whole body, meridians, body, official's key, promotes to weaken.Main manifestations is as follows: insufficiency of kidney-YANG can not replenishing vital QI with drugs of warm nature waist mansion and skeleton, visible soreness of the waist and knees cold type of pain; Insufficiency of kidney-YANG can not warm fur and women's head-ornaments extremity, visible pale complexion, aversion to cold and cold limbs; Insufficiency of kidney-YANG can not be inspired enthusiasm, visible lethargy; Insufficiency of kidney-YANG can not warm spleen soil, and visible chronic diarrhea is more than, aqueous-grainy diarrhea or Diarrhoea; . insufficiency of kidney-YANG finds expression on tongue arteries and veins, and the visible fat tongue of light red tongue is white, and deep-slow pulse is unable " simultaneously, kidney storing essence, a main reproduction! Main water liquid metabolism again, insufficiency of kidney-YANG, can cause the metabolism disorder of water liquid and Reproductive Performance to go down: insufficiency of kidney-YANG, wing skin gasification function obstacle, stops in water liquid, and overflowing in skin is edema; Under water is wet, become, the kidney place part of the body cavity below the umbilicus, housing the bladder, kidneys and bowels, therefore the edema below the waist is very, finger submerged while pressing; Water is wet to spread unchecked, retardance mechanism of qi, abdominal part distension; Therefore aqueous vapor superinverse is insulted the heart and penetrated lung, sees cardiopalmus cough with asthma, man is emission and spermatorrhea, positive rash; Woman leukorrhagia is rare clearly, and cold womb is infertile; All can there is hyposexuality in men and women.

Tcm symptom, sign have fuzzy feature, are subject to the impact of the factors such as subjectivity and weather, environment, body constitution larger.Documents and materials research at all times and completed the dialectical level of factor of nephrasthenia syndrome and pass judgment on the basis of operation standard, extract 40 symptoms, the sign that can reflect syndrome of deficiency of kidney-YANG, and score by rules is revised repeatedly, make the dialectical factor of syndrome of deficiency of kidney-YANG and the score by rules that comparatively meet clinical practice, with the frequency of occurrences of the each dialectical factor of qualitative score calculation, carry out hierarchical cluster analysis with quantitative scoring, draw the primary symptom that feel cold in fear of cold, cold extremities, back, soreness of waist and knee joint can be used as insufficiency of kidney-YANG, cluster analysis prompting insufficiency of kidney-YANG main symptom, picture of the tongue, pulse condition distribute more regular.But it is few that result of study is investigated quantity, interference factor (data are subject to age, Living Water equality) is more, lacks the impact of the factors such as Normal group.

Chamber does not save, overstrain is the Important cause of disease that causes insufficiency of kidney-YANG.Chamber, or claim sexual life, refer to men and women's sexual intercourse process.Labor refers to fatigue, and tired finger is tired weary, no matter be physical exertion or mental work, or chamber hyperkinesia all can cause overstrain.Appropriateness is physical, mental and chamber is movable good for health, and not restraining or excessive, is the major incentive that disease occurs.The traditional Chinese medical science thinks, chamber does not save, overstrain is to cause the two large causes of disease of suffering from a deficiency of the kidney.Chamber activity can be regarded shape labor (physical work) and the refreshing labor (ergasia) of specific form as, too much chamber activity, except labor shape, a tax on one's mind, also directly cause the too much dissipation of kidney essense, of long duration just there will be kidney essense consume.Kidney essense is not only the mankind and is multiplied the source of new life, and is the most important material base of the vital movement day after tomorrow.Deficiency of kidney-essence, will cause physical weakness, the appearance of even multiple deficient property syndrome and disease.Observing from Point of View of Clinical, usually there is waist knee joint weakness in the excessive people of chamber, and dizziness and tinnitus is forgetful weak, dim complexion, frequent micturition, man's sun rash, seminal emission, spermatorrhea, woman's menoxenia, the inferior symptom of cold womb band.

The traditional Chinese medical science thinks that appropriate physical exertion can make that vital essence flows, QI and blood is fluent, contributes to keep body Mental Health.But physical work is too many; do not had a rest in time, cause body too to work hard or hyperkinesia, on the one hand; can cause yang-energy consumption; yang-energy person, bothers and opens, and is exactly that institute's overstrain can make yang-energy speed outside Zhang Yu; and too much dissipate; i.e. " overexertion leading to consumption of QI ", QI consumed directly translates into hurriedly pant (the lung qi consumption) in physical work or later, sweating more (defending gas consumption); pulse frequency is accelerated (motive consumption); limbs fatigue (temper consumption), overstrain, the liver being the dominator of relaxation and contraction of tendons; irritability also consumes; QI consumed is too much, must injure vigour eventually, involves in kidney; On the other hand, often can cause muscles and bones muscle injury, miserable pain of moving part etc. is as main manifestations take the whole body after physical work, extremity, back etc.; Because kidney governing bones makes kidney qi consumption wound excessively, with the passing of time involve kidney yang, can form insufficiency of kidney-YANG.

Chinese medicine thinks that kidney storing essence is the congenital foundation.The main growth of kidney, growth and reproduction, the raw marrow of main bone lead in brain, brain is the place of marrow and gods high concentration, all thinking activitiess of people are all arranged by brain, the essence that "Nei Jing" is said " the not raw marrow of kidney is discontented " kidney is the source of people's vital movement, it is the base substance that forms human body, essence energy activating QI, gas energy spermatogenesis, kidney spermatogenesis, smart hemopoietic, oligospermia are sick, and essence is died to the greatest extent, and the essence gas that declines is weak, the sufficient gas of essence is strong, weakness of QI also a little less than, arrogant blood is prosperous, qi being the governor of blood, blood being the material foundation of QI, visible essence, gas, blood are mutual dependence for existencies.Cloudy essence is that yang-energy is basic.Yang transforming qi, yin shaping up body, cloudy essence and blood homology, as the shape of depletion of YIN essence, just be not enough to carry yang-energy, so sun is born in the life of thing; The one-tenth of thing, is formed in the moon, only has cloudy essence sufficient, and yang-energy is vigorous, and the two coordinated balance, and human body could be healthy.If congenital defect, will lose and support the day after tomorrow, or prolonged illness consumption wound, kidney store essential substances deficiency, " water loses its source, and the disease of the deficiency of YIN is folded out on the one hand; Fire declines it, yang deficiency levy storied." can not go up as suffered from a deficiency of the kidney warm in the heart, fill in brain just there will be cardiopalmus, uncomfortable in chest, breathe hard, insomnia and dreamful sleep, dizziness headache, forgetful.The raw obstacle of QI and bloodization just there will be menoxenia, pachylosis.The imbalance of YIN and YANG of kidney just there will be hectic fever, flushed face in the afternoon, sore waist and aching in the waist and the back, sexual hypofunction and climacteric syndrome etc.

Divide from material and function, it is cloudy that material belongs to, and function belongs to sun, all functional activities of human body all belong to yang-energy, all constitutes of human body all belong to cloudy essence, but in human body, do not have a kind of gas of immaterial pure function, and in Chinese medicine, gas is the cereal nutrient that a kind of vigor is very strong.Therefore gas is the dialectical unity of material and function.Kidney qi is given birth to by kidney essense, and broad sense kidney qi refers to the functional activity of kidney, has comprised kidney yin, kidney yang.Narrow sense kidney qi refers to the part that plays astringency inducing, the effect of envelope Tibetan in kidney, and instability of kidney QI generally, refers to narrow sense kidney qi.Kidney yin is a part for renal function activity, play make moist, Thyme Extract.The implication of usual said kidney yin has also comprised kidney essense in fact interior, sometimes specially refers to kidney essense by kidney yin.Therefore the implication of negative and positive is different in different linguistic context.Sometimes represent all functions activity of human body with yang-energy, sometimes singly refer in bodily fuctions's activity and play part warm, impetus, therefore will note differentiating.Negative and positive can refer to bring into play in qi of zang-fu viscera the gas of adverse effect, and also can make a comment or criticism gas and pathogen also can refer to clearing heat in QI system and foul smell.Cloudy gas does not always represent bad, disadvantageously in a word, and yang-energy is always not good yet.Mention cloudy gas and always do not think pathogenic QI in the body, the pathogen of the human body just referring to, it can also refer to a requisite part in human body physiological function activity.

Kidney is hidden first acquired essence, and kidney essense turns to kidney qi, wherein to body have warm, excite, excited, evaporate, seal and hide and restrict the actor such as YIN-cold and be referred to as kidney yang, be also called Yuanyang, kidney-Yang, kidney-yang; To body have moist, quiet, being shaped and suppressing excessively the actor such as sun heat is referred to as kidney yin, is also called Kidney-Yin, Kidney-Yin, true water.Kidney yang can promote that the metabolism of human body is gasification, promotes the change of essence and blood body fluid give birth to and make it to be converted into energy, and the process of the various physiological activities of human body is accelerated, and heat production increases, in high spirits; Kidney yin suppresses or the excessive metabolic processes of reducing human, making essence and blood body fluid be converted into energy reduces, the process of the various physiological activities of human body slows down, heat production reduces relatively, and make gas be polymerized to shape and be essence and blood body fluid, spirit is also tending towards keeping the two in quiet opposes each other and yet also complement each other, and common adjusting controlled the function activity of human body and the metabolic process of essence and blood body fluid.According to the yin-yang theory of Chinese medicine, negative and positive have limitlessly detachable, and kidney is hidden first acquired essence, and it is cloudy that essence belongs to, so claim cloudy essence, QI belonging to YANG, so claim yang-energy, is collectively referred to as cloudy the vivid YANG QI.Kidney essense belongs to cloudy essence, and forked rubbing more again among cloudy essence, wherein can be divided into again cloudy smart the yang aspect of yang essence and cloudy smart the yin aspect of yin essence.When cloudy smart the yang aspect of yang damage of essence is empty, there are soreness of waist and knee joint, the spinal column Symptoms such as sour tired out, the fatiguability of weak, bone that wither, the Symptoms such as cloudy smart the yin aspect of yin damage of essence has soreness of the waist and knees, talalgia, dry mouth and tougue when empty, quenches one's thirst, insomnia and dreamful sleep.Cloudy smart the yang aspect of yang damage of essence is how in close relations with insufficiency of kidney-YANG when empty, when cloudy smart the yin aspect of yin damage of essence is empty how and deficiency of the kidney yin in close relations.So when insufficiency of kidney-YANG except there being fear of cold to be often afraid of cold etc. performance with soreness of waist and knee joint, the spinal column symptoms such as sour tired out, the fatiguability of weak, bone of withering, when deficiency of the kidney yin except having the performances such as the deficiency of YIN is scorching often with soreness of the waist and knees, talalgia, dry mouth and tougue, the symptom such as quench one's thirst.

Fructus Rubi temperature; Sweet, sour; Return kidney, urinary bladder channel.For kidney tonifying, controlling nocturnal emission with astringent drugs, reducing urination.Treatment enuresis due to deficiency of the kidney, frequent micturition, impotence and premature ejaculation, emission and spermatorrhea." property of medicine opinion ": " main man's kidney-jing deficiency exhausts, woman's food have a son.Main impotence." " Japan hanako materia medica ": " settling five organs, beneficial color, supports vital essence, long hair, strong will.Treat apoplexy fever of the body and frightened." " Kaibao Bencao " carries it: " tonify deficiency is absolutely continuous, reinforcing YIN-essence Jianyang, and smoothing skin, peace and internal organs, warming middle-JIAO benefit power, treats strain wind void, tonifying liver improving eyesight." " Bencao Jingshu ": " Fructus Rubi, its main QI invigorating person, says beneficial vital essence also.Kidney storing essence, kidney improving inspiration by invigorating kidney-QI, vital essence abundance, body, from light, is sent out in vain not also.The respectful main tonify deficiency of reviving is absolutely continuous, reinforcing YIN-essence Jianyang, smoothing skin, peace and internal organs.The main man's kidney-jing deficiency of Zhen Quan exhausts, impotence, woman's food have a son.Large bright main settling five organs, beneficial color, supports vital essence, long hair, strong will.All get its kidney tonifying and add essence, the adopted ear of sweet acid convergence." " explaining abstruseness of the canon of materia medica ": Fructus Rubi, sweet putting down into kidney, plays a sun and controls flaccidity, and controlling nocturnal emission with astringent drugs is taken the photograph excessive, kidney tonifying and without scorching inclined to one side, controlling nocturnal emission with astringent drugs and puckery undoubtedly evil, the product of gold and jade are also.

Radix Polygoni Multiflori Preparata be by Radix Polygoni Multiflori and Semen sojae atricolor with the Radix Polygoni Multiflori drying after boiling, be invigorating the liver and kidney simply, benefiting essence-blood, the good medicine that nourishes heart and allay excitement.Be mainly used in treatment and lose the diseases such as the soreness of the waist and knees that causes, dizziness and blurred vision, palpitation and insomnia, early whitening of hair because of deficiency of the liver and kindey, essence and blood.The black hair function of Radix Polygoni Multiflori is to bring into play by the kidney invigorating, benefit essence, the effect of allaying excitement of nourishing blood.Radix Polygoni Multiflori Preparata has enrich and benefit essence and blood, and nourishing the liver is calmed the nerves, bone and muscle strengthening, and the crow palpus that reinforces the kidney, is shown in Light-headedness for blood deficiency, palpitation and insomnia is forgetful, sallow weak, the vertigo and tinnitus of Liver and kidney blood and essence asthenia, soreness of the waist and knees, seminal emission metrorrhagia and leukorrhagia, the diseases such as early whitening of beard and hair.

Fructus Schisandrae Chinensis temperature; Sour, sweet; Return lung, the heart, kidney channel; Restrain astringent or styptic treatment for spontaneous sweating, supplementing QI for promoting the production of body fluid, kidney calming.For chronic cough dyspnea due to deficiency, emission, enuresis frequent micturition, incessant chronic diarrhea, spontaneous perspiration, night sweat, Tianjin wound is thirsty, the deficient pulse of losing heart, interior-heat is quenched one's thirst, palpitation and insomnia." Bencao Jingshu " carries: the main QI invigorating person of Fructus Schisandrae Chinensis, and all gas of lung master, acid can be received, and is just entering lung tonifying the lung, therefore QI invigorating is also.Its main cough with dyspnea person, the deficiency of vital energy is above stopped up and is not returned unit, and sour flavor having the property of astringing, takes the photograph gas and returns unit, and cough with dyspnea is from removing.Impairment caused by overstrain is won thin, tonifying for the deficiency, reinforcing YIN-essence, beneficial man's essence." not Lu " supports the five internal organs, heat extraction, and flesh person in raw the moon, the special the kidney invigorating of Fructus Schisandrae Chinensis, the tonifying five ZANG-organs of holding concurrently, kidney storing essence, essence is contained cloudy strong, receive to take the photograph vital qi and return unit, and public region is warm, and digesting food, steams waste matter and changes precise and tinyly, smart spontaneous, and essence is raw cloudy long, therefore main as above all diseases are also.

Radix Codonopsis is the conventional traditional tonic of China, and the Radix Codonopsis that produce ancient times take area, Shangdang, Shanxi, as top grade, has invigorating the spleen and replenishing QI, effect of spleen invigorating lung benefiting.Modern study, Radix Codonopsis, containing various saccharides, phenols, sterol, volatile oil, scutellarein glucosid, Saponin and Alkaloid, has enhancing immunity, blood vessel dilating, blood pressure lowering, improves microcirculation, strengthens the effects such as hemopoietic function.In addition leukopenia chemotherapy radiotherapy being caused has castering action.Radix Codonopsis has invigorating the spleen and replenishing QI, effect of spleen invigorating lung benefiting.For deficiency of the spleen and lung, the cardiopalmus of breathing hard, anorexia and loose stool, dyspnea due to deficiency cough, interior-heat is quenched one's thirst etc." Bencao Congxin " recorded: " invigorating the spleen and replenishing QI and taste, relieving thirst and restlessness.A little less than middle feeble QI, mend in order to adjust, very smooth and proper.”

Poria sweet in the mouth, light, property are flat, be used as medicine there is promoting diuresis to eliminate damp pathogen, the function of strengthening the spleen stomach function regulating, mind tranquilizing and the heart calming.Modern medicine study: Poria energy enhancing human body immunity function, pachyman has obvious antitumor and protects the liver dirty effect.For dysuria, disease Poria function promoting diuresis to eliminate damp pathogen such as edema, and the property of medicine is gentle, diuretic and do not hinder healthy energy, is promoting diuresis to eliminate damp pathogen key medicine.The disease of all dysuria, retention of water-damp, no matter be partial to cold-damp, or relatively damp and hot, or belong to insufficiency of the spleen wet poly-, all can fit applications.As cold-damp person relatively, can with the compatibility such as Ramulus Cinnamomi, the Rhizoma Atractylodis Macrocephalae; Be partial to damp and hot person, can with the compatibility such as Polyporus, Rhizoma Alismatis; The person that belongs to deficiency of spleen-QI, can with the compatibilities such as Radix Codonopsis, Radix Astragali, the Rhizoma Atractylodis Macrocephalae; Belong to deficiency and coldness person, also can join Fructus Rubi, the Rhizoma Atractylodis Macrocephalae is equal to use.

Semen Cuscutae is listed in top grade in Shennong's Herbal.Its abnormal smells from the patient is pungent, sweet, flat nontoxic.Function with cure mainly: the kidney invigorating and essence nourishing, nourishing the liver to improve visual acuity.The waist knee joint muscles and bones that is applicable to deficiency of the liver and kindey is ached, the diseases such as legs and feet weakness, impotence and seminal emission, crazy talk, frequent micturition, dripping urination, dizziness and blurred vision, blurring of vision, Hiccough and deaf and women's leukorrhagia, habitual abortion.Generally be listed in top grade can taking for a long time, take for a long time improving eyesight and make light of one's life by commiting suicide and prolong life.But clothes are prohibited in yang deficiency hyperactivity of fire, postcoital protrusion not flaccidity and constipation due to dry stool person, according to individual physique, if there is the sign of getting angry, just cut out.It can cause that sexual function is hyperfunction.

Its sweet in the mouth of Herba Taxilli, the flat effect with the liver and the kidney tonifying of property, say in Compendium of Material Medica and " take for a long time hard muscles and bones, make light of one's life by commiting suicide not old, cold-resistant summer-heat." traditional Chinese medical science conventional it treat hepatic and renal YIN deficiency, soreness of the waist and knees, dizziness, forgetful, dizzy, the many tear of blurred vision, quench one's thirst, the disease such as seminal emission.Modern pharmacology studies confirm that Herba Taxilli scalable body's immunity, can effectively suppress tumor growth and cell mutation, has slow down aging, the effect of anti-fatty liver, adjusting blood fat and the aspect such as blood glucose, promotion hemopoietic function, and is applied to clinical.Herba Taxilli taking convenience, can be used as medicine, chews clothes, steep in wine.But exopathogen excess-heat, insufficiency of the spleen have the wet and person of having loose bowels to avoid clothes.

Radix Rehmanniae Preparata has another name called Radix Rehmanniae Preparata or throws oneself on the ground, belong to goatweed, be a kind of first-class Chinese crude drug, there is the YIN nourishing of enriching blood effect, can be used for blood deficiency and yellow complexion, dizzy, palpitation and insomnia, menoxenia, the diseases such as metrorrhagia, also can be used for hectic fever hectic fever due to YIN-deficiency, the night sweat of deficiency of kidney yin, the disease such as pass out semen, quench one's thirst, is one of deficiency syndrome class nonprescription drugs medicine LIUWEI DIHUANG WAN main component.The all cards of the hepatic and renal YIN deficiency, this quality is moistened into kidney, kind nourishing kidney yin, life essence-filling, marrow-benefitting, is the key medicine of tonifying kidney-yin.Ancients call it " large tonifying five ZANG-organs is Kidney-Yin ", " mending greatly true water ".Normal be equal to use with Rhizoma Dioscoreae, Fructus Corni, treat the hepatic and renal YIN deficiency, soreness of the waist and knees, seminal emission, night sweat, tinnitus, deafness and quench one's thirst etc., can invigorating the liver and kidney, and beneficial marrow, as LIUWEI DIHUANG WAN.

Fructus Corni, cures mainly pathogen cold and heat under the heart, and warming middle-JIAO, by cold warm numbness, goes three worms, the merit that for a long time clothes have been made light of one's life by commiting suicide reinforcing YIN-essence benefit essence, settling five organs, logical nine orifices, stopped dribbling urination; Take for a long time improving eyesight, brute force all the year round.Fructus Corni, has another name called Fructus Corni, medicine Fructus Jujubae, real Fructus Jujubae, Fructus Corni, meat Fructus Jujubae etc., is the conventional rare Chinese medicine of China, and applicating history is long.It is gentle with its benefit power, tonifying YANG and do not help fire, YIN nourishing and oiliness diaphragm, convergence and do not stay the evil special efficacy that waits by successive dynasties use that medical science is liked.Zhang Zhongjing has been formulated " JINGUI SHENQI WAN " take Fructus Corni as monarch.According to chemical analysis, Fructus Corni contains the effective ingredient such as cornin, tartaric acid, gallic acid, malic acid, resin, tannin and the multivitamin that physiologically active is stronger, having the immunity, antiinflammatory of enhancing, the pharmacological action such as antibacterial, is Herba indigoferae Pseudotinctoriae conventional in tcm clinical practice.

Radix Paeoniae Alba nourishing blood to suppress the hyperactive liver, slow middle pain relieving, yin fluid astringing is received antiperspirant.Control the breast abdomen pain over the hypochondriac region, dysentery stomachache, spontaneous sweating, fever due to yin deficiency, menoxenia, metrorrhagia, leukorrhagia." herbal classic ": " main pathogen stomachache, except arthralgia due to stagnation of blood, broken hard long-pending, control cold and heat hernia abdominal mass, pain relieving, diuresis, QI invigorating." " not Lu ": " clear and coherent blood vessels, slow in, dispelling malignant blood, expelling extravasated blood, the gas that anhydrates, sharp bladder, intestine and small intestine, subduing inflammation, row cold and heat when (controlling), attacked by pestiferous factors stomachache, lumbago." " property of medicine opinion ": " and control pathogen of the lung gas, Fu Zhong Xu pain, vim and vigour are gathered, and logical a surname's internal organs are gathered around gas, control evil pain deteriorated blood, disease bone heat when main, strong the five internal organs, the kidney invigorating gas, controls trusted subordinate's heavily fortified point swollen, and married woman's blood closes obstructed, and repercussive blood can lose pus.

Herba Epimedii is pungent, sweet, warm, returns liver, kidney channel.Energy reinforcing the kidney and supporting YANG, strengthening the tendons and bones, expelling wind and removing dampness, is apt to control insufficiency of kidney-YANG, impotence and seminal emission, enuresis frequent micturition; Rheumatic arthralgia, atrophic debility of bones paralysis disease." book on Chinese herbal medicine is stated " cloud " excessive sheep fire, the main impotence of " herbal classic " medicine is hindered absolutely, and " solar corona " be its treatment of head speech man sun absolutely also, and woman is cloudy absolutely, and calling into the gate of vitality, benefit kidney-Yang person is also.The lid gate of vitality be the kidney-Yang in kidney, the vigour of the person also, the exhausted YANG exhaustion the moon of its what is called, the not vigour of basis, how hiss in both withered.So-called physical strength profiting, strong will, and control cold air consumptive disease, and the anxious card that waits of muscles and bones contraction, all it helps the event of vigour.Old man's dusk octogenarian, the middle age is forgetful, all Yuanyang decay and can not ascensionist also.Must know that this taste is to reduce to liter, its liter is owing to falling also.

Rhizoma Polygonati: Rhizoma Polygonati is used as medicine with rhizome.There is boosting qi and nourishing yin, spleen invigorating, lung moistening, kidney benefiting functions.Be used for the treatment of weakness of the spleen and stomach, fatigue and asthenia, xerostomia lack of appetite, deficiency of the lung cough caused by dryness, asthenia of essence and blood, the interior-heat disease such as quench one's thirst.Very effective in cure for diabetes.Rhizoma Polygonati has blood pressure lowering, blood sugar lowering, and blood fat reducing, atherosclerosis, slow down aging and the effect such as antibacterial, polygonatum polysaccharide has Immunestimulatory effect.For lung-dryness syndrome due to deficiency of YIN, the few expectorant of dry cough, and the chronic cough chronic cough of YIN-deficiency of the lung and kidney etc.For weakness of the spleen and stomach.Both tonifying spleen-yin, strengthening the spleen gas again, obtain for deficiency of kidney essence dizzy, soreness of the waist and knees, early whitening of beard and hair and quenching one's thirst etc.Rhizoma Polygonati strengthening bone and muscle, beneficial marrow, bleaches and sends out; Be suitable for cough expectorant due to lack of lung-YIN few, dry cough without phlegm, the diseases such as hemoptysis.

Fructus Rosae Laevigatae is the fruit of rosaceous plant Fructus Rosae Laevigatae, claims again Catunaregam spinosa (Thunb.) Tirveng., thorn pear etc.Fructus Rosae Laevigatae is the famous medicine of the kidney invigorating simply.The traditional Chinese medical science is thought, Fructus Rosae Laevigatae sour in the mouth, and property is flat, enters kidney, large intestine two warps, has reinforcing the kidney and controlling nocturnal emission, effect of reducing urination and antidiarrheal.Can treat emission and spermatorrhea, frequent urination and enuresis, leukorrhagia, metrorrhagia, spontaneous perspiration, night sweat, cough due to deficiency of the lung, insufficiency of the spleen dysentery etc.Modern pharmacology studies confirm that: Fructus Rosae Laevigatae extract can extend the interval time of urinating; Contained tannin has stronger inhibitory action to staphylococcus aureus, escherichia coli, bacillus pyocyaneus etc.Fructus Rosae Laevigatae meridian distribution of property and flavor: sour, sweet, puckery, flat.Return kidney, bladder, large intestine channel.Controlling nocturnal emission with astringent drugs reducing urination, relieving diarrhea with astringents.In Fructus Rosae Laevigatae, contain a large amount of acidic materials, soap one-tenth etc., can controlling nocturnal emission with astringent drugs chamber prevent that pass out semen lingering diarrhea, woman's leukorrhagia of man is too much, again can astringing intestine to stop diarrhea road, prevent the dysentery of insufficiency of the spleen constraint due to ineffective.

Rhizoma Cibotii (EastAsianTreeFernRhizome), pharmacognosy is called Rhizoma Cibotii, is the medicine for rheumatism in Chinese medicine.Bitter, sweet, temperature.Return liver, kidney channel.Invigorating the liver and kidney, except rheumatism, strong waist foot, sharp joint.Control back ache, gonalgia flaccidity of the lower limbs, cold-damp general arthralgia, loses and drowns, frequent micturition, seminal emission, leucorrhea." herbal classic ": main back is strong, machine-operated emergency, general arthralgia cold-damp, gonalgia.Quite sharp old man." not Lu ": treat that losing drowns does not save, man's flaccidity of the lower limbs lumbago, ailment said due to cold or exposure is drenched and is revealed, weak breath order Dark, hard ridge, sharp pitching, woman's damaging the spleen and stomach, joint is heavy." property of medicine opinion ": control man woman poison wind weak foot, pathogen arthralgia chiefly caused by damp pathogen, a little less than deficiency of kidney-QI, tonification man, stricture of vagina muscles and bones." detailed outline ": strong Liver and kidney, strong bone, controls wind void.

Placenta Hominis, has another name called Placenta Hominis, afterbirth etc., is the Placenta Hominis that healthy women is given birth to, through cleaning, smoke dry forming.Placenta Hominis begin to be loaded in supplement to the Herbal upload " main vim and vigour are won thin, married woman's strain, the thin skin of face is black, gradually thin worried person of all diseases in abdomen." traditional Chinese medical science thinks, Placenta Hominis is warm in nature, and sweet-salty enters lung, kidney channel, has the function of strongly invigorating QI and blood, beneficial smart the kidney invigorating, is the tonic of commonly using simply.With its can control QI and blood deficiency, insufficiency of the spleen lack of appetite, cough due to deficiency of the lung, puerperal hypogalactia, seminal emission sexual impotence, leukorrhagia metrorrhagia, the disease such as infertile of miscarrying.Modern pharmacological research shows, Placenta Hominis contains sex gland hormones, ovarian hormone, Alfasone, several amino acids, placental globulin, fibrin stabilizing factor, urokinase inhibitor, plasminogen activator etc.Third kind of placental globulin has the specific function of enhancing human body immunity power, and interferon has antiviral and anticancer effect.Described in comprehensive, Placenta Hominis has the anti-ageing function of good health-care; Often taking it benefits to human body.

Semen Plantaginis returns lung meridian; Liver Channel; Kidney channel; Urinary bladder channel.Function: clearing away heat and promoting diuresis; Eliminating dampness by diuresis antidiarrheal; Improving eyesight; Eliminate the phlegm.Cure mainly: main dysuria; Stranguria with turbid discharge leukorrhagia; Edema distension; Heat-damp in summer dysentery; Conjunctival congestion is hidden from view; Expectorant heat syndrome cough is breathed heavily.1. diuresis, normal person is taken Semen Plantaginis decoct 10g orally, has diuresis, but enters decoct total capacity and every day the water yield all without recording, and lacks strictly contrast.Normal person and rabbit take Herba Plantaginis seed decoct without obvious diuresis.Plantago depressa Willd seed is also without diuresis." property of medicine is fallen ": can remove pathogenic wind toxic, the liver affected by pathogenic wind heat, malicious wind dotting punch order, swelling pain is hidden from view, and headache lacrimation, removes irritable feverish sensation of chest and heart." Japan hanako materia medica ": diuresis drenches puckery, tonifying YANG.Control de-essence, vexed.The therapeutic method to keep the adverse QI flowing downwards.

Fructus Ligustri Lucidi is a kind of conventional Chinese patent medicine, energy nourishing the liver and kidney, strong waist knee joint, and for both liver and kidney deficiency, soreness of the waist and knees, dizzy tinnitus, early whitening of beard and hair, has replenishing kidney-YIN, nourishing the liver to improve visual acuity, except deficiency-heat, the effects such as hearing-improing and eyesight improving.Its property is gentle, and clothes of a specified duration are suitable in nourishing YIN and oiliness stagnant.Fructus Ligustri Lucidi can be treated various disease conditions: reason treatment asthenia.Disease sees that body win is thin, soreness of the waist and knees, and one's ears rang and spots danced before one's eyes, early whitening of beard and hair, dry mouth and throat, feverish sensation in the palms and soles, eyes are dry and astringent dim-sighted, dysphoria and insomnia, red tongue is tender, thready pulse religion or stringy and thready pulse; The 2nd, treatment of insomnia patients.Disease is seen little sleep disturbed by dreams, vexed cardiopalmus, and two are dry and astringent, blurred vision, dizzy tinnitus, feverish sensation in the palms and soles, red tongue, thready and rapid pulse, all neurasthenias and the person that has above-mentioned symptom all can take this side; The 3rd, hair growth disease.Disease sees that beard and hair is uninteresting not flourish, is easy to come off, or early whitening of beard and hair, dizziness and tinnitus, order is puckery dim-sighted, and soreness of waist and knee joint is unable, red tongue, thready and weak pulse; The 4th, treatment seminal emission.Disease is seen soreness of the waist and knees, and cardiopalmus is vexed, dreaminess seminal emission, insomnia forgetfulness, the vertigo; The 5th, treatment hyperlipemia.

The specific embodiment of the invention

Specific embodiment 1: by described raw medicinal material Herba Epimedii 1000g, Fructus Rosae Laevigatae 1000g, Rhizoma Cibotii 1000g, Fructus Ligustri Lucidi 1000g, Placenta Hominis 1000g, Radix Rehmanniae Preparata 1000g, Herba Taxilli 1000g, Semen Cuscutae 1000g, Semen Plantaginis 1000g, Radix Polygoni Multiflori Preparata 1000g, Rhizoma Polygonati 1000g, Radix Codonopsis 1000g, Poria 1000g, Radix Paeoniae Alba 1000g, Fructus Schisandrae Chinensis 1000g, Fructus Rubi 1000g, Fructus Corni 1000g, put into the above Chinese liquor of 70 degree or yellow wine and soak 10～15 days, filter filtrate for later use; Filtering residue decocts with water 2 times, boils 30～40 minutes at every turn, filters; Three filtrates are combined, and heating is concentrated into pasty state, calls in Mel or dextrin, after ultraviolet sterilization, bottles.

Specific embodiment 2: by Herba Epimedii 1200g, Fructus Rosae Laevigatae 1100g, Rhizoma Cibotii 1200g, Fructus Ligustri Lucidi 1200g, Placenta Hominis 1200g, Radix Rehmanniae Preparata 1200g, Herba Taxilli 1100g, Semen Cuscutae 1200g, Semen Plantaginis 1200g, Radix Polygoni Multiflori Preparata 1100g, Rhizoma Polygonati 1200g, Radix Codonopsis 1200g, Poria 1100g, Radix Paeoniae Alba 1200g, Fructus Schisandrae Chinensis 1200g, Fructus Rubi 1100g, Fructus Corni 1200g, in the ethanol that described crude drug adds 5-10 doubly to measure, soak 1-2 hour, heating extraction 2 times, each 1-2 hour, remove supernatant, merge extractive liquid, 100-120 order filters, be the ultrafiltration post ultrafiltration of 5000-10000 again through the molecular weight that dams, 1.36 extractum when ultrafiltrate concentrating under reduced pressure relative density is 80 ℃, heating is concentrated into paste, stand for standby use, become component 1, the medicinal residues of the crude drug of above-mentioned steps ethanol extraction are put into 10 times of water gagings, heating and refluxing extraction 2 times, each extraction time is 1～2 hour, 100-120 order filters, be the ultrafiltration post ultrafiltration of 5000-10000 again through the molecular weight that dams, 1.36 extractum when ultrafiltrate concentrating under reduced pressure relative density is 80 ℃, heating is concentrated into paste, becomes component 2.After being merged, component 1 and component 2 insert in economic benefits and social benefits vacuum concentrator the concentrated solution that while being concentrated into 90 ℃, relative density is 1.05, set to 0～5 ℃ of deepfreezes 24 hours; Cold preservation liquid is added to 0.3% filter aid kieselguhr, filter, filtrate is inserted in economic benefits and social benefits vacuum concentrator again, is concentrated into every 1ml containing 1g crude drug amount; Unguentum after concentrated adds dextrin and is in harmonious proportion, and after ultraviolet ray sterilization bactericidal, bottles.

Specific embodiment 3: by Herba Epimedii 1300g, Fructus Rosae Laevigatae 1400g, Rhizoma Cibotii 1400g, Fructus Ligustri Lucidi 1200g, Placenta Hominis 1200g, Radix Rehmanniae Preparata 1200g, Herba Taxilli 1100g, Semen Cuscutae 1400g, Semen Plantaginis 1200g, Radix Polygoni Multiflori Preparata 1400g, Rhizoma Polygonati 1200g, Radix Codonopsis 1400g, Poria 1300g, Radix Paeoniae Alba 1200g, Fructus Schisandrae Chinensis 1300g, Fructus Rubi 1100g, Fructus Corni 1400g, described crude drug is put into after pulverizer is pulverized and crossed 120 order dusting covers, the fine powder amount that enable pass is crossed 120 mesh sieves is reaching 30%, collects by the raw material fine powder of 120 mesh sieves for subsequent use.Particulate matter remaining after sieving is put into ethanol heating and refluxing extraction 2 times, each 1～2 hour, 2 extracting solution are merged and left standstill; In described step c, the medicinal residues that above-mentioned ethanol extraction is crossed add 10 times of water gaging heating and refluxing extraction 2 times, each 1～2 hour, 2 extracting solution are merged and left standstill, above-mentioned two kinds of extracting solution are merged, and 1.38 filtrate when concentrating under reduced pressure relative density is 70 ℃, reclaims ethanol, call in dextrin or Mel, after ultraviolet sterilization, bottle.

Pharmacology's toxicity test

One, test material:

(1) laboratory animal: mouse NIH, body weight 25-28g, male and female half and half; Rats Wistar body weight 130-160g, male and female half and half, the feedstuff of rat, mice is provided by medical college zoopery center, Shandong.

(2) tested medicine:

1, the made oral liquid of medicine of the present invention: embodiment 2, applies with distilled water diluting when three batch equal amount of mixture experiments.

2, positive control medicine: JINGUI SHENQI WAN, commercially available.

Two, test method and result:

(1) get 80 of the healthy mices of body weight 25-28g, female, male half and half, be divided into respectively four groups by sex and body weight, 20 every group.1, take medicine of the present invention for 2,3 groups, dosage is respectively 8.6g crude drug/kg/d, 6.4g crude drug/kg/d, and 4.2g crude drug/kg/d, takes positive control drug JINGUI SHENQI WAN for the 4th group, and dosage is 6g crude drug/kg/d, is used in conjunction 15 days.

Result of the test: 1,2,3 groups of mices obviously strengthen than 4 groups of mice sexual excitation degree, and the number of copulations of 1,2,3 group of mice increases by 3 times than the number of copulations of 4 groups of mices average every day.

Result of the test shows that medicine of the present invention has obvious excitation to central nervous system, thus medicine of the present invention for regulating human body function equilibrium, QI invigorating, enrich blood, slow down aging strengthens metabolism, neurolysis, sexual hypofunction has treatment foundation.

Animal acute toxicity test of the present invention

One, test material

(1) animal mouse NIH kind male and female half and half, body weight 20-22g, is provided by Shandong medical college Animal Lab..

(2) tested medicine: the oral liquid that the present invention is made, is diluted with distilled water into suspension.

Two, test method and result:

Mice maximum tolerance determination: get 32 of healthy mices, male and female half and half, within 24 hours points three groups, only, gross weight 1.8ml, is equivalent to crude drug in whole 4.0g to the each 0.6ml/ of gavage intestinal, i.e. and 100g crude drug/kg/d is equivalent to 857 times of clinical 65 kilograms of people.After mice administration, feedstuff has no animal dead in 12 days and does not go out, shows toxic reaction routinely.Therefore prove, medicine of the present invention has no side effect, and clinical practice is safe and reliable.

The long-term toxicity test for animals of pharmaceutical composition of the present invention

One, test material and method

(1) tested medicine: the oral liquid that the embodiment of the present invention is made.

(2) laboratory animal, healthy rat Wistar, body weight 100-130g, 140 male and female half and half, by sex and body weight random packet, four groups, 35 every group.1, take tested medicine for 2,3 groups, dosage is respectively 9.6/kg/d, 6.4/kg/d, 3.2/kg/d.4 groups of equal gavage enteral administrations of distilled water, rat that contrast to respective volume amount, every group is once used in conjunction 6 months, puts to death half, and all death in 12 days is observed in remaining 1/2 drug withdrawal.

Two, test index

(1) general status comprises body weight, diet, emotion, movable energy.

(2) hemogram checking: red blood cell count(RBC), numeration of leukocyte, platelet count, hemogram etc., quantitative hemoglobin counting.

(3) biochemical analysis, serum albumin quantitatively, cholesterol, blood sugar quantitative.

(4) pathologic diagnosis: get half animal after experimental result and weigh, sacrificed by decapitation, gets blood, solution is treated others for earnest simultaneously, liver, kidney, lung, brain, spleen, gastrointestinal, bladder, adrenal gland do pathologic diagnosis.

Three, result of the test: show through rat chronic toxicity test, use the heavy dose of coloclysis of the present invention six months, growth, body weight to rat increase, brain, liver, renal function are all had no side effect.Each histoorgan, the blood circulation of rat are not found to infringement changes, and medication group and matched group be no significant difference relatively.Illustrate that medicine of the present invention has warming and recuperating the kidney-YANG, QI invigorating, enriches blood, slow down aging strengthens the drug effects such as metabolism, avirulence.

Clinical trial:

Determination for the treatment of based on pathogenesis obtained through differentiation of symptoms and signs is the marrow of Chinese medicine, the modern study of syndrome is all the focus in traditional Chinese medical science modernization all the time, also be one of difficult point, animal model with TCM syndrome is that Chinese medicine and Modernization of traditional Chinese medicine have been erected the bridge of, makes Chinese medicine stride forward an important step to standardization, controlledization of condition.Animal model is the matter of utmost importance of carrying out animal experiment study, and which kind of method to set up the important step that the animal model with TCM syndrome that meets tcm clinical practice reality is Chinese medicine research by.Insufficiency of kidney-YANG animal model is one of animal model with TCM syndrome that success is set up the earliest, as far back as the sixties in last century, Kuang Guang moulds professor and waits and just set up " hydrocortisone insufficiency of kidney-YANG animal model ", open the beginning of Kidney Theory in TCM yang deficiency Animal Model, for kidney-replenishing method modern medicine Study on mechanism has been created essential condition.Continue since first insufficiency of kidney-YANG Animal Model, insufficiency of kidney-YANG Animal Model research field Showed Very Brisk, the different modeling methods of insufficiency of kidney-YANG and relevant the kidney invigorating research are used and are reported in a large number, according to the preliminary statistics, now existing more than 20 class modeling methods.

It is the critical problem of syndrome of deficiency of kidney-YANG essence research that the success of insufficiency of kidney-YANG animal model copies, and is also the foundation stone of research traditional Chinese medical science the kidney invigorating therapy and formula.The modeling method of " overstrain, chamber do not save and add gentamycin " of the present invention, combines traditional cause of disease modeling with pathology modeling, both followed the content that tcm causa morbi is learned completely, has again the advantage of pathology modeling concurrently." overstrain ", or " chamber does not save " is all the clinical modal tcm causa morbi that causes insufficiency of kidney-YANG, gentamycin simultaneously that use, also be one of reason causing clinical common renal damage, this make this model compared with in other existing model theories closer to Kidney-Yang Deficiency Syndrome clinically.The corresponding clinical manifestation that insufficiency of kidney-YANG occurs and the variation of associated hormone level, have important value to research syndrome of deficiency of kidney-YANG essence.Male reproductive function is low, and it is one of clinical insufficiency of kidney-YANG patient common manifestation that sperm quality declines.So, to the detection of rats with deficiency of kidney-Yang sperm quality, can directly reflect the weak degree of rats with deficiency of kidney-Yang Reproductive Performance.After medication, can reflect the improvement situation of Experimental agents to sperm quality.Shenqi pill is compared emphatically in this experiment, and the present invention two sides improve situation to reproduction, to disclosing the two in difference aspect the kidney invigorating from 2 aspects.

Materials and methods

1, material (l) animal: healthy male Wistar rat, body weight 200 ± 2g; Adult female rat, 90.Provide (animal licence: SCX2007012743) by Shandong Traditional Chinese Medicine University's Experimental Animal Center.Normal diet: produced the quality certification number by Shandong Province's animal science institute: Shandong animal feed 200802031.(2) main agents estradiol valerate (progynova): branch company of French first miraculous cure industry group (Guangzhou) produces, lot number: 03713-2; Gentamycin: Shandong Lu Kangchenxin Pharmaceutical limited company produces, specification: 2ml:8 ten thousand u/ prop up " batch number: the accurate font size H37021973 of 01124306 traditional Chinese medicines.Picric acid, heparin, normal saline, platform are expected blue solution, TYH culture fluid, the preparation of hospital preparation center, the attached middle Shandong of distilled water Deng Dong university of TCM.(3) Chinese medicine preparation, the preparation of hospital preparation center, the attached middle Shandong of university of TCM, medicine and dosage are as follows:

Shenqi pill: Radix Rehmanniae 40g, Rhizoma Dioscoreae 20g, mountain English meat 20, Rhizoma Alismatis 15, Poria 15g, Cortex Moutan 15g, Cortex Cinnamomi 5g, gun additioner 5g.

Oral liquid of the present invention: Herba Epimedii 12g, Fructus Rosae Laevigatae 13g, Rhizoma Cibotii 12g, Fructus Ligustri Lucidi 11g, Placenta Hominis 11g, Radix Rehmanniae Preparata 12g, Herba Taxilli 13g, Semen Cuscutae 11g, Semen Plantaginis 12g, Radix Polygoni Multiflori Preparata 13g, Rhizoma Polygonati 12g, Radix Codonopsis 11g, Poria 11g, Radix Paeoniae Alba 12g, Fructus Schisandrae Chinensis 12g, Fructus Rubi 13g, Fructus Corni 13g.

(4) instrument: key instrument: microscope: binocular microscope PHSO mono-16O0x manufacturer: Shanghai Rui Bo educational facilities company limited.General Instrument: syringe, irrigation stomach device, micro sample adding appliance, ice machine, homogenizer, EP pipe, mosquito forceps etc.The court provides.

2. method

(l) rutting period female Mus preparation

All 90 female Mus, every day gavage estradiol valerate suspension lml, and confirm " (estradiol valerate suspension compound method: lmg estradiol valerate is dissolved in to the female two liquor-saturated solution 120ml of valeric acid that make 3.3% in 30ml distilled water; every ml is containing crude drug 0.033mg, good the day use) with vaginal smear examination keratinocyte

(2) male Mus grouping

Male Mus grouping adopts table of random number method, 40 of male rats are divided into 4 groups at random: blank group (then be all called for short blank group), overfatigue, chamber do not save and add gentamycin kidney-yang deficiency model group, (then be all called for short model group), shenqi pill treatment group (then be all called for short shenqi pill group), treatment group of the present invention (then be all called for short invention group), 10 every group.

(3) modeling

Each group experiment is carried out 5 weeks altogether, blank group: routine feeding 2 weeks; Every gavage tap water 4ml/d later; maintain 3 weeks, model group: experiment l week: make every male Mus and 3 same cages of female Mus in rutting period, and change female Mus in rutting period every day; do not save (if finding pregnant female Mus, the female Mus in rutting period more renewing immediately) with induction chamber; Test the 2nd week: at depth of water 50cm; 30 ℃ of water temperatures; in the swimming trunk of volume 0.36ml, force every male Mus swimming, swim every day 1 time; duration 1 hour; if find, rat is exhausted, after sinking can not on while emerging, picked up rapidly " it is taken a momentary rest; then drop into again in water, to induce overstrain; And coordinating every male Mus lumbar injection gentamycin 150mg/kg every afternoon simultaneously, rear every day, gavage tap water 4ml/d, maintained 3 weeks.Shenqi pill group: modeling method is with above-mentioned model group, every gavage shenqi pill suspension 4ml/d (containing crude drug 0.55g) after 2 weeks, this method perfusion maintains 3 weeks.Of the present invention group: modeling method is with above-mentioned model group, every gavage suspension 4ml/d of the present invention (containing crude drug 0.55g) after 2 weeks, this method perfusion maintains 25 days.

(4) ordinary circumstance observation

Record body weight, figure, fur glossiness, a perpendicular hair phenomenon, whether fear of cold be afraid of cold, whether like crowded together, the whether shrinkage of reaction sensitivity, autonomic activities situation, diet, defecation situation, scrotum, and one record swimming time.

(5) specimen collection

Rats by intraperitoneal injection pentobarbital that (100mg/kg), anaesthetizes, after anaesthetizing successfully, cut, take out rapidly two kidneys, the emerald green ball of bilateral and attached kingfisher, peeling outer layer tissue and peplos, wash away bloodstain with normal saline, filter paper blots after surface moisture, weighs with analytical balance.

(6) testing process of sperm quality

Sperm count: sperm suspension splashes in cell counting pond, carries out sperm count by automatic counting of red blood.With the sperm count of 5 medium squares in the large lattice of high power lens counting central authorities.

Sperm motility degree is analyzed: under 25 ℃～28 ℃ room temperatures, get the attached tail same area of starting of a side, the attached tissue of starting of the 100mg that accurately weighs, puts into the vial of 2ml normal saline 37 ℃ of water-bath preheatings is housed, and shreds into sperm suspension.In 37 ℃ of water-baths, leave standstill after 2min, draw 0.01ml, drip immediately on the blood cell counting plate of 37 ℃ of preheatings, under microscope, observe fast, with reference to motility of sperm grade scale, record the quantity of motile sperms at different levels in 200 left and right sperms, obtain its percentage rate.

Sperm survival rate is observed: adopts Yihong test, gets sperm suspension and live each 1 mixing of 5% Yihong aqueous solution, and 305 rear high power lens inspections, the sperm of living is not painted, dead sperm is dyed pale red or faint yellow, calculates 200 sperm survival rates "

Sperm Morphologic Study: suction pipe is drawn sperm suspension, filter through 4 layers of lens paper, draw filtrate smear, dry rear with the fixing smin of methanol, 2% Yihong dyeing 1h, water fine laundering, dry, under microscope mirror, every animal checks 200 1 500 complete sperms (every treated animal is at least counted 1000 complete sperms), obtains rate of teratosperm.

(7) statistical procedures

Application SPSS13.0 Chinesizing version software carries out date processing, observes each index and all uses t check, and data represent with-x ±-s.

(2) result

1. ordinary circumstance

Model group compares with blank group: the obvious weak asthenia of model group animal, lassitude, and it is blunt gradually to react, and autonomic activities also reduces in a large number; Hogback, roll up, visible multiple rats flock together, and are afraid of cold performance obviously; Hair is comparatively sparse, and fur glossiness is poor, often occurs obviously perpendicular hair phenomenon (swimming is later common); Feed, amount of drinking water all reduce; Urine amount increases (by the dry wet degree judgement of bedding and padding in cage) gradually, has thin when stool; The swimming time of adhering to progressively shortens, and because fatigue is not propped up, causes " touching the end " also to increase gradually.

Of the present invention group: food-intake increases compared with model group, shenqi pill group, body weight slightly increases compared with model group, shenqi pill group, and pharmaceutical intervention later stage perpendicular hair phenomenon reduces gradually, the perpendicular hair of part rat phenomenon disappears, fur gloss is better, and autonomic activities is obviously more, and when swimming, anti-fatigue ability is stronger.

Shenqi pill group: still compared with normal group is on the low side for food-intake, loses weight, has perpendicular hair phenomenon, but is improved compared with model group, and the sparse phenomenon of hair is not obvious, and fur glossiness makes moderate progress compared with model group, and autonomic activities is general, and when swimming, anti-fatigue ability is poor.

2. testis, kidney weight

The comparison of table 1 one 1 rats with deficiency of kidney-Yang testis, kidney weight (g)

Group	Testicular weight	Kidney weight
			Blank group	3.5144±0.2255*	2.6667±0.2598
Model group	3.25±0.2268	2.9063±0.6304
			Shenqi pill group	3.5875±0.2395*	3.075±0.4773
Of the present invention group	3.734±0.3317	2.98±0.5404

Note: testicular weight: with model group comparison .P<0.05

From table 11, model group is compared with blank group, testicular weight obviously reduces, there is statistical significance (P<0.05),. shenqi pill group, of the present invention group and model group comparison, testicular weight all obviously increases. all there is statistical significance (P<0.05), but shenqi pill group and of the present invention group of comparison, the increase not statistically significant (P>0.05) of guilt ball weight.Illustrate that shenqi pill, the present invention all have the effect that increases rats with deficiency of kidney-Yang testicular weight, kidney weight is respectively organized comparison, all not statistically significant (P>0.05).Therefore, illustrate that this experimental model and pharmaceutical intervention group have no significant effect rat kidney total amount.

3, sperm quantity, sperm motility.

The comparison of table 1 one 2 rats with deficiency of kidney-Yang sperm quantities (105), sperm motility

Note: sperm quantity: with model group comparison, * P<0.05; Sperm motility: with model group comparison, #P<0.01, with the comparison of shenqi pill group, ##P<0.05

From table l 1, model group rat spermatozoa quantity, sperm motility is starkly lower than blank group and (is respectively P<0.05, P<0.01), of the present invention group of sperm quantity and activity ratio all (are respectively P<0.05 higher than model group, P<0.01), shenqi pill group activity ratio is significantly higher than model group (P<0.01), sperm quantity is improved trend compared with model group, but not statistically significant (P>0.05), and of the present invention group of sperm motility is higher than shenqi pill group (P<0.05).

Illustrate that shenqi pill, the present invention all can improve rat spermatozoa quantity and activity ratio, and effect of the present invention is better than shenqi pill.

4. sperm survival rate, abnormal rate

The comparison of table 1 one 3 rats with deficiency of kidney-Yang sperm survival rates, rate of teratosperm

Group	Sperm survival rate	Rate of teratosperm
			Blank group	79.2222±4.3237#	2.11±1.25##
Model group	65.875±3.9074	3.25±0.5244
			Shenqi pill group	72.63±5.5946*	2.24±1.5572
Of the present invention group	78.23±5.3184#	2.33±1.0000&

Note: sperm survival rate: with model group comparison, #P<0.01, * P<0.05, rate of teratosperm: with model group comparison, ##P<0.05, & P<0.02 is from table 11, model group rat spermatozoa survival rate is significantly lower than blank group (P<0.01), rate of teratosperm is higher than blank group (P<0.05), shenqi pill group, of the present invention group and model group comparison, sperm survival rate (is respectively P<0.05 higher than model group, P<0.01), of the present invention group is relatively improved the trend of sperm survival rate with shenqi pill group, " of the present invention group of rate of teratosperm is starkly lower than model group (P<0.01) to but not statistically significant (P>0.05), shenqi pill group is improved not obvious (P>0.05) to rate of teratosperm.Visible, shenqi pill, of the present invention group all can improve sperm survival rate, reduce abnormal rate, but effect of the present invention are slightly better than shenqi pill.

Three, rats with deficiency of kidney-Yang effect of Sex Gland Hormone assay

Gonad axis associated hormone, promoting to have very important effect in emerald green ball sexual cell growth and development process, maintains the stable of emerald green ball spermatogenesis process simultaneously.Therefore, further investigate the mensuration of different the kidney invigorating methods to rats with deficiency of kidney-Yang gonad axis associated hormone content, on disclosing different the kidney invigorating methods, the impact of sexual cell and spermatogenesis process is had to most important theories and clinical value.

(1) materials and methods

1. material

(l) animal is with experiment one

(2) reagent: rat is started ketone (T), rat follotropin (FSH), rat lutropin (LH),

EUSA detection kit: ADL company product.

(3) key instrument is with experiment two

2. method

(l) animal grouping, model copy, administration etc. are all with experiment one.

(2) after experiment modeling, medication finishes the rear blood of getting respectively, measures T, FSH, LH, and experimental procedure is basic with experiment two

(3) statistical procedures

Adopt SPSS13.0 software to process, the t check of two groups of comparisons, data represent with-x ±-s

(2) result

1. the variation of testosterone (T)

The comparison of the each group testosterone (ng/mI) of table 31 level

Group	After modeling	After medication
			Blank group	8.5884±1.7983	9.8363±1.0709
Model group	6.6727±0,985*	6.8565±1.3841
			Shenqi pill group	6.5038±0.9885*	7.7422±1.1804
Of the present invention group	6.8065±1.1767*	8.1707±1.3827#

Note: after modeling, with the comparison of blank group, * P<0.05; Of the present invention group, before and after medication, compare #P<0.05.From table 31, three groups of modelings, testosterone levels all decline (P<0.05), after medication, shenqi pill group highland ketone level has rise trend, but not statistically significant (P>0.05), and of the present invention group of testosterone levels recovers obviously (P<0.05) after compared with modeling, illustrate that the present invention has restitution to rats with deficiency of kidney-Yang highland ketone level, and effect is better than shenqi pill.

2. the variation of Folliculogenesis element (FSH)

The comparison of the each group Folliculogenesis element (Iu/L) of table 31 level

Group	After modeling	After medication
			Blank group	5.2334±1.9621	5.3687±1.705
Model group	3.1773±0.988*	3.1859±1.1164
			Shenqi pill group	2.797±1.5736*	4.8526±1.8632
Of the present invention group	3.1747±1.9081	5.0912±1.1892#

Note: after modeling, with the comparison of blank group, * P<0.05, before and after the medication of Yougui Wan, the kidney-Yang-Reinforcing Bolus group, compare, #P<0.05 is from table 31, after modeling, can cause rat Folliculogenesis element level and be starkly lower than blank group (P<0.05), after medication, after shenqi pill group and modeling of the present invention, relatively rats with deficiency of kidney-Yang FSH there is the trend of recovery with model group, but not statistically significant (P>0.05), before and after of the present invention group of medication, relatively FSH level is recovered obviously (P<0.05), with after the medication of shenqi pill group, relatively rise is more obvious, but not statistically significant (P>0.05).Illustrate that modeling process can make rat FSH level decline, the present invention has restitution to rat FSH level, and effect is better than shenqi pill.

3. the variation of lutropin (LH)

The comparison of table 3 one 3 lutropins (mIu/ml) level

Group	After modeling	After medication
			Blank group	3.9215±0.7149	4.1127±0.9956
Model group	3.1496±0.521*	2.8463±1.84

Shenqi pill group	3.1525±0.5289*	3.4198±0.9299
			Of the present invention group	3.0273±0.8903*	3.6354±0.5635

Note: after modeling, with the comparison of blank group, * P<0.05 is from table 31, and after modeling, each group rat LH level, lower than blank group (P<0,05), has statistical significance.After medication, shenqi pill group, of the present invention group of LH level all have rise trend, but compare not statistically significant (P>0.05) respectively with before medication.Illustrating that modeling process can cause rat LH level to decline, may there is the effect that recovers LH level in shenqi pill, of the present invention group, but the latter two no significant differences (P>0.05) relatively of medication.

Discuss

By modeling, model group animal occurs that the yang deficiency such as obvious weak asthenia, active movement minimizing promote unable caused performance; , also there is due to kidney yang deficiency unable the rousing oneself of the mind and occur that lethargy is depressed, the performances such as bradykinesia meanwhile; Hogback, roll up, assemble wait the performance of being afraid of cold belong to yang deficiency can not be warm not as good as due to; Hair is sparse, and fur is owed gloss etc. for suffering from a deficiency of the kidney for a long time, fur lose in moisten foster due to; Feed, amount of drinking water all reduces, just the pool may be because insufficiency of kidney-YANG, and YANG failing to warm spleen-YANG, due to dysfunction of the spleen in transportation; Urine belongs to insufficiency of kidney-YANG more, can not promote the circulation of qi and water due to.Pharmaceutical intervention group, experimental rat Kidney-Yang Deficiency Syndrome is all improved in varying degrees, and is now compared as follows: treated animal autonomic activities of the present invention, fur gloss intensity, especially food-intake improvement is better than shenqi pill group.Shenqi pill treated animal food-intake is even not as good as model group animal, and no significant difference is improved in other aspects.Illustrate that shenqi pill, the present invention all can improve the symptom of the insufficiency of kidney-YANG of experimental rat in various degree, and effect of the present invention is slightly better than shenqi pill group.

From shenqi pill and prescription angle of the present invention, invigorating middle warmer of the present invention has rushes down, residence rushes down in benefit, Poria can play function of invigorating spleen, and " the spleen being the foundation of acquired constitution, source of generating QI and blood, if the strong fortune of spleen and stomach function; not only can make QI and blood abundance; also can supplementing nutrition the day after tomorrow, and play the effect of indirect the kidney invigorating, " from this angle, the present invention has effect of spleen reinforcing concurrently.

Semen quality is one of important indicator of male reproductive function.It detects and mainly comprises sperm quantity, activity ratio rate of teratosperm etc.From the experimental results, shenqi pill, the present invention all can comparatively significantly increase the emerald green ball weight of rat, and kidney weight is had no significant effect.The two increase to testicular weight is no significant difference (P>0.05) relatively.Shenqi pill, the present invention all can improve rat spermatozoa quantity, survival rate, enhancing sperm motility, and as can be seen here, shenqi pill, ball of the present invention all can improve rat spermatogenesis ability, and effect of the present invention is more obvious compared with shenqi pill.

Spermatogenesis is the process of hormone dependence, successively hypertrophy and a differentiation.Testicular spermatogenic function is mainly subject to the regulation and control of hypothalamus one hypophysis one gonad axis.The gonadoliberin (GnRH) of the position skin serotonergic neuron secretions such as nucleus arcuatus hypothalami, directly act on adenohypophysis through hypophyseal portal system, promote that Adenohypophysial goadotrophic cells is synthetic and secrete FsH, LH and T etc., participate in adjusting sexual function and reproductive function, to maintain the dynamic equilibrium of whole period of duration.FSH is except the division and early stage Development of spermatogenic cell of direct startup spermatogonium, also can be combined with sustenticular cell membrane receptor, stimulate the generation of androgen-binding protein (ABP), ABP is combined with T, can maintain the T high concentration level in convoluted seminiferous tubule, promote that germline is ripe sperm.

LH claims again interstitial cell stimulating hormone, can be specific and Interstitial cell receptors bind, and protein mediated by G, make CAMP in cell generate increase, accelerate the phosphorylation process of endocellular function protein, cause cholesterol ester hydrolysis to strengthen, and promote cholesterol to enter the synthetic testosterone of mitochondrion.Meanwhile, LH also can make the enzyme activity of being combined with T in Interstitial cell increase, thereby accelerates the synthetic of T.T after Interstitial cell secretion, can with sustenticular cell receptors bind, and change the emerald green ketone of two hydrogen into, promote that " in spermatogenesis process, T plays important maintaining and regulating action for the differentiation of spermatogenic cell and the generative process of sperm.Test shows that, after sustenticular cell excessive Apoptosis, in blood, T level obviously declines, and causes a large amount of apoptosis of sexual cell, gives T and can prevent most of apoptosis of germ cells.Therefore, T can regard the major hormone regulation and control person that apoptosis of germ cells occurs as.

Therefore FSH, LH, T hormone test can be used to judge the spermatogenesis state of emerald green ball.In this experiment, shenqi pill, the present invention all can promote the recovery of rats with deficiency of kidney-Yang FSH, T level, and effect of the present invention is slightly better than shenqi pill.Modern pharmacological research confirmation, in the present invention, Placenta Hominis used can directly act on adenopituicyte, promotes the release of T, LH.And LH level is had no significant effect, may be caused by experimental implementation error.Experimental result prompting, shenqi pill and the present invention improvement to semen quality, may lead to the improvement of FSH, T is realized.And the present invention is because of containing more Zishen fill fine medicine, semen quality is improved and the restitution of gonadal hormone FSH, T etc. is slightly better than to shenqi pill.

Claims (10)

1. treat the Chinese medicine preparation of insufficiency of kidney-YANG for one kind, it is characterized in that, the weight fraction of described Chinese medicine preparation crude drug comprises: 10～20 parts of Herba Epimedii, 10～20 parts of Fructus Rosae Laevigatae, 10～20 parts of Rhizoma Cibotii, 10～20 parts of Fructus Ligustri Lucidi, 10～20 parts of Placenta Hominiss, 10～20 parts, Radix Rehmanniae Preparata, 10～20 parts of Herba Taxillis, 10～20 parts of Semen Cuscutae, 10～20 parts of Semen Plantaginiss, 10～20 parts of Radix Polygoni Multiflori Preparata, 10～20 parts of Rhizoma Polygonatis, 10～20 parts of Radix Codonopsis, 10～20 parts, Poria, 10～20 parts of the Radix Paeoniae Albas, 10～20 parts of Fructus Schisandrae Chinensis, 10～20 parts of Fructus Rubies, 10～20 parts of Fructus Corni.

2. treat according to claim 1 the Chinese medicine preparation of insufficiency of kidney-YANG, it is characterized in that, the weight fraction ratio of described Chinese medicine preparation crude drug is: 10～15 parts of Herba Epimedii, 10～15 parts of Fructus Rosae Laevigatae, 10～15 parts of Rhizoma Cibotii, 10～15 parts of Fructus Ligustri Lucidi, 10～15 parts of Placenta Hominiss, 10～15 parts, Radix Rehmanniae Preparata, 10～15 parts of Herba Taxillis, 10～15 parts of Semen Cuscutae, 10～15 parts of Semen Plantaginiss, 10～20 parts of Radix Polygoni Multiflori Preparata, 10～20 parts of Rhizoma Drynariae, 10～20 parts of Radix Codonopsis, 10～20 parts, Poria, 10～20 parts of the Radix Paeoniae Albas, 10～20 parts of Fructus Schisandrae Chinensis, 10～20 parts of Fructus Rubies, 10～20 parts of Fructus Corni.

3. treat according to claim 1 the Chinese medicine preparation of insufficiency of kidney-YANG, it is characterized in that, the weight fraction ratio of described Chinese medicine preparation crude drug is: 10～20 parts of Herba Epimedii, 10～20 parts of Fructus Rosae Laevigatae, 10～20 parts of Rhizoma Cibotii, 10～20 parts of Fructus Ligustri Lucidi, 10～20 parts of Placenta Hominiss, 10～20 parts, Radix Rehmanniae Preparata, 10～20 parts of Herba Taxillis, 10～20 parts of Semen Cuscutae, 10～20 parts of Semen Plantaginiss, 10～15 parts of Radix Polygoni Multiflori Preparata, 10～15 parts of Rhizoma Polygonatis, 10～15 parts of Radix Codonopsis, 10～15 parts, Poria, 10～15 parts of the Radix Paeoniae Albas, 10～15 parts of Fructus Schisandrae Chinensis, 10～15 parts of Fructus Rubies, 10～15 parts of Fructus Corni.

4. a Chinese medicine preparation for the treatment of insufficiency of kidney-YANG as described in any one in claim 1～3, is characterized in that, the preparation process of described Chinese medicine preparation comprises:

A. described crude drug is put into ethanol heating and refluxing extraction 2 times, as component 1;

B. get the medicinal residues after above-mentioned ethanol extraction, the heating and refluxing extraction that adds water 2 times, as component 2 again;

C. above-mentioned two kinds of extracting solution components 1 and component 2 are merged, after concentrating, add dextrin and be in harmonious proportion, after disinfection, be prepared into oral solutions.

5. the preparation method for the treatment of according to claim 4 the Chinese medicine preparation of insufficiency of kidney-YANG, is characterized in that, in described step a, in the ethanol that adds 5-10 doubly to measure described crude drug, soak 1-2 hour, heating extraction 2 times, each 1-2 hour, remove supernatant, merge extractive liquid,, 100-120 order filters, then is the ultrafiltration post ultrafiltration of 5000-10000 through the molecular weight that dams, 1.36 extractum when ultrafiltrate concentrating under reduced pressure relative density is 80 ℃, heating is concentrated into paste, and stand for standby use becomes component 1.

6. treat according to claim 4 the preparation method of the Chinese medicine preparation of insufficiency of kidney-YANG, it is characterized in that, in described step b, the medicinal residues of the crude drug of above-mentioned steps ethanol extraction are put into 10 times of water gagings, heating and refluxing extraction 2 times, each extraction time is 1～2 hour, 100-120 order filters, then is the ultrafiltration post ultrafiltration of 5000-10000 through the molecular weight that dams, 1.36 extractum when ultrafiltrate concentrating under reduced pressure relative density is 80 ℃, heating is concentrated into paste, becomes component 2.

7. treat according to claim 4 the preparation method of the Chinese medicine preparation of insufficiency of kidney-YANG, it is characterized in that, in described step c, after component 1 and component 2 are merged, insert in economic benefits and social benefits vacuum concentrator, the concentrated solution that while being concentrated into 90 ℃, relative density is 1.05, set to 0～5 ℃ of deepfreezes 24 hours; Cold preservation liquid is added to 0.3% filter aid kieselguhr, filter, filtrate is inserted in economic benefits and social benefits vacuum concentrator again, is concentrated into every 1ml containing 1g crude drug amount; Unguentum after concentrated adds dextrin and is in harmonious proportion, and after ultraviolet ray sterilization bactericidal, bottles.

8. as described in any one in claim 1～3, treat insufficiency of kidney-YANG Chinese medicine preparation a preparation method, it is characterized in that, its preparation process can also be:

A. described crude drug is pulverized, sieved;

B. the granule sieving is soaked, is extracted 3 times;

C. 3 concentrated proper honey of sneaking into of extract are in harmonious proportion, after ultraviolet ray sterilization bactericidal, bottle.

9. the preparation method for the treatment of according to claim 8 the Chinese medicine preparation of insufficiency of kidney-YANG, is characterized in that, it is characterized in that, in described step a, get crude drug clean dry, in 70 ℃ of baking ovens, dry 8-12h, take out and be placed in drying baker coolingly, pulverized 80-100 mesh sieve with pulverizer.

10. the preparation method for the treatment of according to claim 8 the Chinese medicine preparation of insufficiency of kidney-YANG, is characterized in that, it is characterized in that, in described step b, the particulate matter that sieves is soaked in water 12 hours, then puts into extraction pot heated and boiled 1 hour, filters filtrate for later use; Filtering residue adds water, and heating, boils 45 minutes for the second time, filters filtrate for later use; Filtering residue adds water again, heated and boiled half an hour for the third time, filters; Three filtrates are combined, powder is placed in 70-90% ethanol and soaks 1-2 hour, is heated to 60 ℃-70 ℃, lixiviate 2h in water-bath, every 10min stirs once, by filtered through gauze, in residue, add 50-60% ethanol, 60 ℃-70 ℃ are continued lixiviate 2h, every 10min stirs once, filtered through gauze, merges lixiviating solution, is condensed into pasty state.