CN103776992A - Monitoring method for imidacloprid drug resistance of apple aphis citricola - Google Patents

Monitoring method for imidacloprid drug resistance of apple aphis citricola Download PDF

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Publication number
CN103776992A
CN103776992A CN201410043998.3A CN201410043998A CN103776992A CN 103776992 A CN103776992 A CN 103776992A CN 201410043998 A CN201410043998 A CN 201410043998A CN 103776992 A CN103776992 A CN 103776992A
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apple
imidacloprid
drug
spiraea aphid
finger
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CN201410043998.3A
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Chinese (zh)
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王俊平
郑长英
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Qingdao Agricultural University
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Qingdao Agricultural University
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Abstract

The invention relates to a monitoring method for imidacloprid drug resistance of apple aphis citricola. The method comprises the following steps: (1) dissolving raw drug imidacloprid into acetone to be diluted into five concentrations; (2) injecting a diluted drug solution into the bottom of a finger-shaped tube; inclining, laying flatly and rotating the finger-shaped tube; (3) slightly picking and rotating the apple aphis citricola to the finger-shaped tube by a writing brush and checking a result to obtain LC60, LC70, LC80 and the like; (4) uniformly distributing the imidacloprid with the concentrations of the LC60, the LC70 and the LC80 into the finger-shaped tube; slightly picking and rotating the apple aphis citricola to the finger-shaped tube by the writing brush and primarily determining the diagnosis dosage and the conformity; and (5) determining the imidacloprid drug resistance of the apple aphis citricola in the field. The monitoring method utilizes the glass finger-shaped tube so that the accuracy of a resistance monitoring result is improved, the usage amount of the drug is reduced and the cost is saved. According to the monitoring method, a detection kit is suitable for directly determining imidacloprid drug resistance of the apple aphis citricola in orchards; the operation is simple and convenient and the result can be rapidly determined.

Description

A kind of apple spiraea aphid is to the drug-fast monitoring method of Imidacloprid
Technical field
The present invention relates to a kind of apple spiraea aphid to the drug-fast monitoring method of Imidacloprid, belong to Pesticide Science technical field.
Background technology
Apple spiraea aphid (Aphis citricola Vander Goot) belongs to Homoptera (Homoptera) Aphidiadae (Aphididae aphids), main host plants has the plants such as plant on apple, pears, mountain, meadow sweet, is one of the primary pest of fruit tree of causing harm in northern China orchard.Yang Junyu etc. (2013) claim that all there is generation apple spiraea aphid each province, and it is Hebei, Henan, Shanxi and Shandong that serious province occurs, and Gansu, Liaoning, Yunnan and Shaanxi occur lighter; Pesticide probability of use is respectively Imidacloprid, Avermectin and effective cypermethrin from high to low.Because chemical prevention is for many years main, cause it to produce certain resistance to the action of a drug.In order to delay the drug-fast development of apple spiraea aphid, formulate in time resistance management measure, monitoring for resistance becomes accurately knows the effective means of apple spiraea aphid at somewhere resistance level.At home and abroad the initial report of Brader in 1977 about the apple spiraea aphid resistance to the action of a drug, Yao Hongwei (2002) has done guestimate to the Homoptera resistant insect kind of reporting in the world, and wherein in Korea S, oneself has produced resistance to organophosphorus and carbamic acid vinegar insecticides to apple yellow aphid.The resistances to the action of a drug of ground apple spiraea aphid to fenvalerate and flolimat such as Cangzhou, Hebei, Shangqiu, Henan, Mengyin, Shandong, Chaoyang, Taian Shandong, Changli, Hebei, Dangshan, Anhui, Shandong Rong Cheng that Wang Jinxin etc. (1998) etc. have adopted topical application method.Xu Qin etc. (2002) have adopted topical application method in Shanxi, Xinzhou and Yuncheng three ground apple yellow aphids measure the resistance to the action of a drug of 7 kinds of chrysanthemum ester insecticides.Peng Bo etc. (2010) adopt drop method, to Weifang City of Shandong Province, Yantai City, Tai’an, Rongcheng City, Wendeng City, Longkou City, Laixi City, Pingdu City, Fei County, Mengyin County, Qixia City and Yiyuan County and other places, alphamethrin, Imidacloprid, flolimat and Acetamiprid have been carried out to resistance to the action of a drug mensuration.Envelope clouds (2012) adopt infusion process, to Yuncheng, Shanxi area apple spiraea aphid, Acetamiprid, Imidacloprid, Biphenthrin, effective cypermethrin, fenvalerate, phoxim and malathion have been carried out to resistance to the action of a drug mensuration.
The resistance to the action of a drug mensuration work of above-mentioned apple spiraea aphid is really for its field control and resistance management provide great help, but assay method adopts drop method and infusion process more, exists operation to be difficult for grasping and the loaded down with trivial details and nonstandard problem of method.
Summary of the invention
The object of the invention is to the deficiency existing in existing monitoring method, on the basis of existing research method for a kind of easy, resistance monitoring method fast and accurately, to meet the needs of current orchard monitoring for resistance.
To achieve these goals, technical scheme of the present invention is as follows.
A kind of apple spiraea aphid is to the drug-fast monitoring method of Imidacloprid, and the method comprises the following steps:
(1) former medicine Imidacloprid is dissolved into and in acetone, is diluted to 5 concentration;
(2) at the bottom of dilute liquid medicine injects and refers to type pipe, careful tilt to set level rotate dactylethrae until dripless, room temperature is placed 2h;
(3) apple spiraea aphid is gently chosen and gone to finger type pipe with writing brush, check result after certain hour, obtains LC 60, LC 70and LC 80deng, tentatively obtain alternative diagnostic dose;
(4) with LC 60, LC 70and LC 80the even spread of Imidacloprid of concentration, in finger type pipe, is gently chosen apple spiraea aphid and is gone to finger type pipe with writing brush, and check result after certain hour is tentatively determined diagnostic dose and degree of conformity;
(5) utilize diagnostic dose to measure the resistance to the action of a drug of field apple spiraea aphid.
Further, in described step (2), dilute liquid medicine injects and refers to type pipe (length * internal diameter=75mm*16mm), and volume number is 220uL, put down gently flat, now liquid just in time flow to the mouth of pipe and not excessive, and room temperature is rolled, until acetone all volatilizees.And the resistance to the action of a drug that can carry out apple spiraea aphid after room temperature is placed 2h is measured.
Further, in described step (3), apple spiraea aphid is gently chosen from foot, is positioned in finger type pipe, can not injure lancet.Room temperature is observed apple spiraea aphid death condition after placing 2h, and apple spiraea aphid can not freely be creeped and is death, freely creeps and is survival.
This beneficial effect of the invention is:
(1) the present invention uses glass to refer to type pipe (length * internal diameter=75mm*16mm), is beneficial to evenly adhering to of medicament, has improved the accuracy of resistance monitoring result; The use amount that simultaneously reduces medicament is cost-saving.
(2) the apple spiraea aphid resistance to the action of a drug of the present invention is measured kit, is suitable for directly measuring the resistance to the action of a drug of apple spiraea aphid to Imidacloprid in orchard, simple to operate, convenient, Fast Measurement result.
Embodiment
Below in conjunction with embodiment, the specific embodiment of the present invention is described, to better understand the present invention.
Embodiment
A kind of apple spiraea aphid, to the drug-fast monitoring method of Imidacloprid, specifically comprises following aspect:
(1) the preliminary of kit diagnostic dose determined:
Take calculated mass and be 96% the former medicine of Imidacloprid of 0.1042g, use 100mL acetone solution, be made into the Imidacloprid mother liquor of 100mg/L, become 25.6,12.8 by acetone diluted afterwards, 6.4,1.6,0.4 and 0.1mg/L, acetone is contrast, each processing repeats for 3 times.Then at the bottom of referring to type pipe with the injection of micropipettor absorption 220uL liquid (75mm*16mm), dactylethrae is set level in careful inclination, liquid anchors at along with the volatilization of acetone on the whole inwall of glass tube, and after acetone all volatilizees, glass tube medicine film is placed room temperature 2h.Gently choose apple spiraea aphid (Qingdao Agricultural University experimental plot) and put into pipe, in every pipe 15～20, cover the mouth of pipe with gauze.After processing, examination worm is placed on interior raising of incubator of 25 ℃ of temperature, photoperiod L: D=14h: 10h, checks examination worm death toll and the number of surviving after 2h, calculates LC according to virulence equation 50, choose continuous three LC 50the result approach the most, measurement result being stable is carried out data merging, then calculates LC 60, LC 70and LC 80.
This time test is due to the fast detecting fruit that only comes to an end after 2h, therefore concrete dead criterion used is extremely important while inspection, through test of many times finally settle the standard for: apple spiraea aphid can not be creeped and is death, freely creeps and is survival.
Result is as follows:
Adopt dactylethrae medicine embrane method to carry out biologicall test to Qingdao Agricultural University living collection apple spiraea aphid population, choose continuous three LC 50the result approach the most, measurement result being stable is carried out data merging (in table 1), adopts Probit software to carry out data processing, calculates respectively LC 60, LC 70, LC 80corresponding concentration, and as alternative diagnostic dose.
Table 1:
Measure the date Regression equation LC 50(mg/L) 95% fiducial interval
7.28 Y＝4.596+0.888X 2.851 1.854～4.515
7.29 Y＝4.654+0.884X 2.460 1.583～3.863
7.30 Y＝4.349+1.024X 4.315 2.793～7.485
Pooled data Y＝4.603+0.882X 2.814 2.191～3.646
LC 60, LC 70and LC 80be respectively 5.45mg/L, 11.05mg/L and 25.28mg/L
(2) the preliminary of diagnostic dose determined and degree of conformity check:
According to the LC calculating in step (1) 60, LC 70and LC 80, concentration is carried out the preparation of glass tube medicine film, the same step of method (1), the each repetition of every processing 3 times.After 2h, check examination worm death toll and survival number, calculate respectively and obtain corrected mortality.Check the degree of conformity of each diagnostic dose.Calculate respectively acquisition corrected mortality according to corrected mortality formula.Check the degree of conformity of each diagnostic dose, specifically in table 2.
Table 2:
Diagnostic dose Dose concentration (mg/L) Corrected mortality (%)
LC 60 5.45 57.07
LC 70 11.05 71.38
LC 80 25.28 75.48
As known from Table 2, can establish LC 70concentration (11.05mg/L) is as diagnostic dose
(3) diagnostic dose is measured the susceptibility of field apple spiraea aphid:
According to the LC calculating 70concentration is carried out the preparation of glass tube medicine film, the same step of method (1).Examination worm is the Mouping area apple spiraea aphid (resistance) in apple orchard and the apple spiraea aphid of Qingdao Agricultural University's living collection (relative responsive).3 processing are respectively the apple spiraea aphid in apple orchard, area, Mouping, and the apple spiraea aphid of Qingdao Agricultural University's living collection and Mouping and Qingdao apple spiraea aphid respectively account for 50%, the each repetition of every processing 3 times.After 2h, check examination worm death toll and survival number, calculate respectively and obtain corrected mortality.The degree of conformity of checking each diagnostic dose, concrete outcome is in table 3.The apple spiraea aphid field population in apple orchard, area, Mouping is tested, to the LC of Imidacloprid 50for the 41.91mg/L (LC of Qingdao apple spiraea aphid to Imidacloprid 50for 12.83mg/L), be about 3.27 times of relative sensitive population.
Table 3:
Examination worm LC 70Mortality ratio (%) Contrast dead rate (%) Corrected mortality (%)
Mouping 22.41 2.22 20.64
Qingdao 70.47 2.08 69.85
50% (trying to gain+green grass or young crops) 39.58 0 39.58
Because LC 70imidacloprid concentration is processed respectively Mouping and Qingdao apple spiraea aphid, and the mean value of its corrected mortality is 45.25%.This value is greater than LC 70imidacloprid concentration is processed mixed population (Mouping: Qingdao=1: corrected mortality 1), illustrates that Mouping population has certain resistance to the action of a drug.Data declaration LC 70this diagnostic dose is to be applicable to very much field diagnosis.
The above is the preferred embodiment of the present invention; it should be pointed out that for those skilled in the art, under the premise without departing from the principles of the invention; can also make some improvements and modifications, these improvements and modifications are also considered as protection scope of the present invention.

Claims (3)

1. apple spiraea aphid, to the drug-fast monitoring method of Imidacloprid, is characterized in that: the method comprises the following steps:
(1) former medicine Imidacloprid is dissolved into and in acetone, is diluted to 5 concentration;
(2) at the bottom of dilute liquid medicine injects and refers to type pipe, tilt to set level to rotate dactylethrae until dripless, room temperature is placed 2h;
(3) apple spiraea aphid is gently chosen and gone to finger type pipe with writing brush, check result, obtains LC 60, LC 70and LC 80deng, tentatively obtain alternative diagnostic dose;
(4) with LC 60, LC 70and LC 80the even spread of Imidacloprid of concentration, in finger type pipe, is gently chosen apple spiraea aphid and is gone to finger type pipe with writing brush, and check result is tentatively determined diagnostic dose and degree of conformity;
(5) utilize diagnostic dose to measure the resistance to the action of a drug of field apple spiraea aphid.
2. apple spiraea aphid according to claim 1 is to the drug-fast monitoring method of Imidacloprid, it is characterized in that: in described step (2), dilute liquid medicine injects and refers to type pipe (length * internal diameter=75mm*16mm), volume number is 220uL, put down gently flat, now liquid just in time flow to the mouth of pipe and is not excessive, room temperature is rolled, until acetone all volatilizees, and the resistance to the action of a drug of carrying out apple spiraea aphid after room temperature is placed 2h is measured.
3. apple spiraea aphid according to claim 1, to the drug-fast monitoring method of Imidacloprid, is characterized in that: in described step (3), apple spiraea aphid is gently chosen from foot, is positioned in finger type pipe, can not injure lancet; Room temperature is observed apple spiraea aphid death condition after placing 2h, and apple spiraea aphid can not freely be creeped and is death, freely creeps and is survival.
CN201410043998.3A 2014-01-26 2014-01-26 Monitoring method for imidacloprid drug resistance of apple aphis citricola Pending CN103776992A (en)

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Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2003055118A (en) * 2001-08-09 2003-02-26 Bayer Cropscience Kk Pest-controlling method
CN103529194A (en) * 2013-10-12 2014-01-22 中国农业大学 Method for detecting drug resistance of green peach aphid

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2003055118A (en) * 2001-08-09 2003-02-26 Bayer Cropscience Kk Pest-controlling method
CN103529194A (en) * 2013-10-12 2014-01-22 中国农业大学 Method for detecting drug resistance of green peach aphid

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
GRAHAM D. MOORES ET AL: "Characterisation of Insensitive Acetylcholinesterase in Insecticide-Resistant Cotton Aphids, Aphis gossypii Glover (Homoptera: Aphididae)", 《PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY》 *
李结平等: "几种杀虫剂对梨绣线菊蚜和中国梨木虱的毒力及药效", 《中国南方果树》 *
陈川等: "几种药剂对苹果绣线菊蚜的防效试验", 《陕西农业科学》 *

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Inventor after: Wang Junping

Inventor after: Zheng Changying

Inventor after: Shi Guanghua

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Inventor before: Zheng Changying

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Application publication date: 20140507