CN103775951A - Light-emitting diode (LED) array based standard plane light source - Google Patents
Light-emitting diode (LED) array based standard plane light source Download PDFInfo
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- CN103775951A CN103775951A CN201210403202.1A CN201210403202A CN103775951A CN 103775951 A CN103775951 A CN 103775951A CN 201210403202 A CN201210403202 A CN 201210403202A CN 103775951 A CN103775951 A CN 103775951A
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Abstract
The invention discloses an LED array based standard plane light source. The standard plane light source comprises a casing, an LED glowing array, an LED brightness control module, an interface module and a power supply module. The casing guarantees that no light rays leak, the LED glowing array is arranged in the casing, the LED brightness control module is disposed in the casing and controls and adjust each LED in the LED glowing array, so that the maximum error of LED brightness values in the LED glowing array is smaller than 1%, the interface module is arranged in the casing and in communication connection with the LED brightness control module. The interface module is arranged in the casing and connected with the LED brightness control module to achieve communication between the LED brightness control module and an upper control system, and the power supply module is arranged in the casing to provide the stable working voltage for composing parts of the standard plane light source. According to the standard plane light source, the glowing array is formed by the LEDs, so that the standard plane light source is stable during usage, high in uniformity, small in size and practical, low in cost and convenient to operate.
Description
Technical field
The present invention relates to the standard flat light source of proofreading and correct between a kind of homogeneity correction for bioprotein chip reading instrument and instrument, be specifically related to a kind of standard flat light source based on LED array.
Background technology
Biological chip reading apparatus, by biochip is taken, according to the gray value of calibration curve and sample spot, calculates the concentration value of each sample.
The camera system of biological chip reading apparatus is made up of monochromatic CCD and camera lens, because shooting distance is restricted, in coverage, cannot obtain the extraordinary image of uniformity (error of centerand edge is 10%～15%), so need to carry out inhomogeneity correction to camera system.Meanwhile, in the time that instrument is produced, the instrument of new production and reference instrument need to be carried out to Concordance, at this moment also need to use standard sources.
Because the development difficulty of standard flat light source is high, a lot of technological difficulties cannot overcome, and therefore adopt at present spot light to carry out the Concordance between homogeneity correction and the instrument of instrument.Adopt the favourable part of spot light to be: volume is little, place flexibly, applied widely; Existing problems are: complicated operation, and location trouble is proofreaied and correct at every turn, and spot light need to constantly move 48 positions, and each position needs to take a pictures.Carry out homogeneity correction one time, need about 1～2 hour.
For this reason, the present invention design a set of stable, the uniformity is high, the small and exquisite practicality of volume, low cost and easy to operate standard sources, as the calibration tool of biological chip reading apparatus.
Summary of the invention
The present invention is directed to the Concordance between homogeneity correction and the instrument that available technology adopting spot light carries out instrument, the problem such as existing complicated operation, location trouble and efficiency are low, and a kind of standard flat light source based on LED array is provided.This standard flat light source forms light emitting array by LED, stable when use, the uniformity is high, the small and exquisite practicality of volume, low cost and easy to operate.
In order to achieve the above object, the present invention adopts following technical scheme:
A standard flat light source based on LED array, described standard flat light source comprises:
Housing, described housing guarantees dull thread leakage;
LED light emitting array, described LED light emitting array is placed in housing;
LED brilliance control module, described LED brilliance control module is placed in housing, and controls to adjust the each LED in LED light emitting array, makes the LED brightness value worst error in LED light emitting array be less than 1%;
Interface module, described interface module is placed in housing, and joins with LED brilliance control module communication, realizes communicating by letter of LED brilliance control module and upper control system;
Power module, described power module is placed in housing, for the building block of standard flat light source provides stable operating voltage.
In preferred embodiment of the present invention, described LED light emitting array is set to 8 × 6 arrays according to the form of protein chip.
Further, described LED brilliance control module comprises CPU and LED brightness regulation chip, described CPU communicates by interface module and upper control system, and control connection LED brightness regulation chip, and described LED brightness regulation chip controls connects the each LED in LED light emitting array.
Further, described LED brightness regulation chip is controlled the brightness of LED by current control mode and frequency control system.
Further, described current control mode is undertaken by following formula:
I
real=I
if× R/63
In formula: I
real-real work electric current; I
if-electric current; R-operating current arranges.
Further, described frequency control system is undertaken by following formula:
H
real=H
if× M/4095
In formula: H
real-actual operating frequency; H
if-setpoint frequency; M-operating frequency arranges.
Further, described power module comprises lithium battery, power switch chip, voltage-stabilizing device and CPU, and the Voltage-output of described voltage-stabilizing device control lithium battery, guarantees the voltage that its stable output is consistent; The Voltage-output of described power switch chip controls lithium battery; Described CPU detects the output voltage of lithium battery in real time by A/D modular converter, the output voltage that lithium battery detected as CPU is when requiring, and standard of closure light source, has so just guaranteed that each start can obtain consistent operating voltage automatically; Described CPU detects that by time block the working time is more than or equal to the automatic shutdown time of setting, and just powered-down switch chip automatically, realizes automatic shutdown.
The standard flat light source obtaining according to such scheme forms light emitting array by LED, adopts LED array to replace original light source, and its uniformity height very meets the requirement of demarcating completely; And each LED can regulate separately its brightness, thereby make the LED brightness value worst error of whole array be less than 1%; Standard flat light source, with serial ports, can arrange the brightness of each LED by computer software; Regulative mode is divided into electric current and regulates and frequency adjustment, and degree of regulation is high; Adopt high-precision voltage stabilizing device, while needing only lithium battery output voltage higher than 3.0V, the output voltage peak-peak error after voltage stabilizing is less than 0.5%; Whole light source has a style of one's own, compact conformation, and volume is little, can be placed in the sensing chamber of bioprotein chip reading instrument, does not destroy darkroom seal.
Accompanying drawing explanation
Further illustrate the present invention below in conjunction with the drawings and specific embodiments.
Fig. 1 is schematic diagram of the present invention;
Fig. 2 is structural front view of the present invention;
Fig. 3 is structure top view of the present invention;
Fig. 4 is structure upward view of the present invention;
Fig. 5 is structure rearview of the present invention;
Fig. 6 is structure upwarding cutaway view of the present invention;
Fig. 7 is circuit board top view of the present invention;
Fig. 8 is the block diagram of upper control system in the present invention;
Fig. 9 is the structural representation of LED light emitting array in the present invention.
Identifier declaration in figure:
100-standard flat light source; 101-housing; 102-LED light emitting array; 103-LED brilliance control module; 104-interface module; 105-power module; 201-upper cover; 202-bottom; 203-cover plate; 204-light-transmissive film; 205-diffuser plate; 206-attenuator; 207-grid film; 208-circuit board; 209-battery; 210-power switch; 211-RS232 interface; 212-communication state indicating module; The preheating of 213-light source arranges module; 214-electric current regulates module is set; 215-frequency adjustment arranges module; 216-automatic shutdown arranges module.
The specific embodiment
For technological means, creation characteristic that the present invention is realized, reach object and effect is easy to understand, below in conjunction with concrete diagram, further set forth the present invention.
Referring to Fig. 1, the standard flat light source 100 based on LED array provided by the invention, it mainly comprises that one does not reveal the housing 101 of light, LED light emitting array 102, LED brilliance control module 103, interface module 104 and power module 105 as light emitting source.
The arrangement main body that housing 101 is whole planar light source, it must assurance not reveal corresponding light except the parts for carrying composition planar light source simultaneously.
Wherein LED light emitting array 102 is arranged in housing, and this LED array is corresponding with the structure of bioprotein chip in whole test specification, and each LED has represented the region of a person-portion, adopts separation measure simultaneously, and adjacent LED can not be influenced each other.
LED brilliance control module 103 is placed in housing, for controlling to adjust each LED of LED light emitting array, makes the LED brightness value worst error in LED light emitting array be less than 1%.
In order to have realized, the brightness of each LED is regulated separately, brilliance control module in the present invention is made up of CPU and special LED brightness regulation chip, CPU is the parameters receiving from upper control system (being upper control computer), be converted into control signal and send special LED brightness regulation chip to, complete the real-time control to LED by this chip, the largest benefit of design is the resource that does not take CPU like this, and control accuracy is directly controlled high a lot than CPU.
Specific implementation is as follows:
Open standard sources power supply, each LED be placed into successively protein chip reading apparatus under, observe its position by chip reading instrument, guarantee the center (computer display interface at protein chip reading apparatus on have center mark) of tested LED in taking, then take according to identical shooting time, obtain its gray value, by brightness regulation software, gray value to tested LED regulates, and makes it reach setting value.The error of having guaranteed LED gray value in whole standard sources by such method is less than 1%.
In order to guarantee the precision to LED brightness regulation, LED brightness regulation chip adopts electric current to regulate and two kinds of modes of frequency adjustment to LED.
Upper control system described herein is for realizing the self energy control of standard flat light source, and it is implemented based on upper computer, makes user can simply complete fast the functions such as brightness regulation, preheating setting and automatic shutdown.Between computer and standard flat light source, adopt standard RS232 interface, each communication result is all presented on software interface, if Communications failure will have obvious prompting.
In the present invention, this power module is also one of important step of guaranteeing the work of standard flat light stability, and it realizes two functions: guarantee output voltage one after the each start automatic shut-off function of making peace.Adopt high-precision voltage-stabilizing device at power unit, guaranteed that the output voltage error after each start is less than 0.5%.Because the volume of standard flat light source is very limited, and the power consumption of LED is larger, even if adopt jumbo lithium battery, stream time is also more limited.The requirement using according to reality, has designed the function of automatic shutdown in the present invention, and by the CPU self-clocking in brilliance control module, according to the unused time of setting, automatic cut-off power, has extended the access times of standard flat light source greatly.On the one hand, owing to having adopted high-quality voltage-stabilizing device, make to change battery the brightness of LED is not had any impact in addition.
Concrete, this power module comprises lithium battery, power switch chip and voltage-stabilizing device.Voltage-stabilizing device adopts high-precision LDO, and LDO is the voltage-stabilizing device of high accuracy, high stability, as long as meet: the condition (lower than the maximum input voltage value of device regulation) of input voltage >=output voltage+0.2V, just can guarantee the voltage that stable output is consistent.In order to guarantee that each start can obtain consistent output voltage, utilizing CPU(in brilliance control module to adopt CPU in brilliance control module to control not only reduces costs, can also reduce the use of components and parts, make product with compact structure) A/D translation function cell voltage is detected, the output voltage that battery detected as CPU is when requiring, automatically standard of closure light source, has so just guaranteed that each start can obtain consistent operating voltage.Utilize the timing function of same CPU, when detecting that the working time is more than or equal to the automatic shutdown time that user sets, just automatically powered-down switch chip, realizes the function of automatic shutdown simultaneously.
Based on such scheme, specific embodiment of the invention is as follows:
Referring to Fig. 2 to 7, in instantiation, adopt upper cover 201, bottom 202 and cover plate 203 to form housing, upper cover 201 matches and joins with bottom 202, between forms the space of each parts of settling composition planar light source, cover plate 203 is placed in bottom 202 simultaneously, and the placement hole on bottom 202 is carried out to capping.The housing forming thus, except work required, avoid the leakage of other all light, guarantee the accuracy of measurement result.
For guaranteeing the integrally-built compactness of planar light source, reduce its volume, in this example by integrated the control section except battery in LED light emitting array 102, LED brilliance control module 103, interface module 104 and power module 105 formation one circuit board 208, design feature and the function of each functional module in this circuit board 208 (LED light emitting array 102, LED brilliance control module 103, interface module 104) are same as described above, are not repeated herein.
Accordingly, the middle part in this circuit board 208 arranges the LED of 8 × 6 arrays according to the form of protein chip as shown in Figure 7, forms corresponding LED light emitting array 208a; Be formed with RS232 interface 211 in one end of circuit board 208 as corresponding interface module, be provided with corresponding power switch 210 at the other end of circuit board 208 simultaneously, the work by this power on-off control circuit plate with stop; On circuit board 208, be also integrated with the respective chip that forms LED brilliance control module 103, in figure, do not show.
The accommodation space forming of joining the shape of circuit board 208 and upper cover 201 and bottom 202 matches, and is placed between upper cover 201 and bottom 202, and wherein upper cover 201 is pressed on circuit board 208 upside of bottom 202 inside.
Because the luminous of bioprotein chip is extremely faint, therefore on LED, need to cover attenuator its light is weakened, make it approach the brightness of biochip.For this reason in this example, above circuit board 208, paste one deck grid film 207, above circuit board 208, be provided with light-transmissive film 204, diffuser plate 205 and attenuator 206 simultaneously, wherein light-transmissive film 204, diffuser plate 205 and attenuator 206 fit together successively from top to bottom, and are fixed on the inside of upper cover plate 201.Attenuator 206 can arrange two-layer as required.
The effect of grid film 207 is to separate LED light emitting array, reduces influencing each other between LED; Light-transmissive film 204 is the films that have 48 apertures, and effect is the light emitting region of controlling LED; The effect of diffuser plate 205 is that the light that LED is sent is decentralized, homogenising; The effect of attenuator is the luminosity that reduces LED, makes the luminous intensity of standard sources approach the luminous intensity of protein chip.
The LED light emitting array forming thus as shown in Figure 9, lighttight grid film 207 is installed being provided with between the circuit board 208 of LED light emitting array and attenuator 206, grid film 207 is made up of whippy, lighttight elastomeric material, according to the size of LED light emitting array, have 48 square openings, mutually isolated light tight between each hole.Grid film 207 fits tightly with attenuator 206 and wiring board 208, so just each LED is separated, and makes not influence each other between LED.
The battery 209 of parts is provided as working power, and it is placed on below circuit board 208, joins, and be encapsulated in the inside of planar light source by being fixed on the cover plate 203 of bottom 202 bottoms with the control assembly on circuit board.
The planar light source finished product forming according to such scheme leaks for fear of light, impacts measuring, and power switch and communication interface all can be positioned over to the side of light source shell, and adopt necessary means to avoid the leakage of light.The planar light source forming like this has a style of one's own, compact conformation, and volume is little.
The upper control system being used in conjunction with this planar light source in this example has communication state indicating module 212, light source preheating and module 213, electric current are set regulate and arrange that module 214, frequency adjustment arrange module 215, automatic shutdown arranges module 216.
Communication state indicating module 212 is used to indicate serial communication state, and communication success or failure all show by this module.
Light source preheating arranges module 213 for carrying out the pre-thermal parameter setting of standard sources, and preheating time (0～5 minute), preheat curent (0～63) and pre-heat frequency (0～4095) can be set.
Electric current regulates and module 214 is set for to single led operating current setting, and by following formula, the real work electric current of LED is calculated:
I
real=I
if× R/63
In formula:
I
real-real work electric current.
I
if-set electric current, arranged by outer meeting resistance, the setting electric current in the present invention is 2mA.
R-operating current arranges, and adjustable range is 0～63.
Frequency adjustment arranges module 215 for single led operating frequency setting, and by following formula, actual operating frequency is calculated:
H
real=H
if× M/4095
In formula:
H
real-actual operating frequency.
H
if-setpoint frequency, is arranged by the software of brightness regulation module, and the setting electric current in the present invention is 1K Hz.
M-operating frequency arranges, and adjustable range is 0～4095.
Automatic shutdown arranges module 216 for automatic shutdown setting, and it is 0～999 minute that automatic shutdown time arranges scope, and in the time inputting 0, standard sources can automatic shutdown.
Be divided into brightness setting, Source calibration and three parts of normally working by the concrete operation of above-mentioned this example flat of control system control light source, detailed process is as follows:
(1) brightness setting:
Use RS232 interface and the computer that is mounted with upper control system on connection connection standard planar light source, open corresponding upper control system.When brightness arranges, can realize single led brightness is regulated carrying out, also can regulate all LED simultaneously.
LED brightness example 1(is set single led brightness is set):
Step 1: by control is set accordingly, select the number of corresponding LED
Step 2: regulate the electric current and the frequency parameter that module 214 and frequency adjustment are set module 215 is set revises these LED by electric current;
Step 3: electric current regulates and module 214 and frequency adjustment are set module 215 is set calculates final control signal according to the parameter arranging and reach on planar light source by connection;
Step 4: the CPU of the control LED on planar light source receives after parameters, is converted into control signal and sends special LED brightness regulation chip to, completes the real-time control to corresponding LED by this chip.
LED brightness example 2(is set whole LED brightness is set):
Step 1: by control is set accordingly, select the number of whole LED
Step 2: regulate the electric current and the frequency parameter that module 214 and frequency adjustment are set module 215 is set revises all LED by electric current;
Step 3: electric current regulates and module 214 and frequency adjustment are set module 215 is set calculates final control signal according to the parameter arranging and reach on planar light source by connection;
Step 4: the CPU of the control LED on planar light source receives after parameters, is converted into control signal and sends special LED brightness regulation chip to, completes the real-time control to all LED by this chip.
(2) demarcation of standard sources:
By each LED be placed into successively special bioprotein chip reading instrument under, then its brightness is measured, regulate according to measurement result, make its measured value in 2000 ± 10 scope.After 48 LED all regulate, just complete the correction of standard sources.
(3) course of work:
Step 1: press the power switch 210 on standard flat light source
Step 2: standard flat light source is positioned in bioprotein chip reading instrument sensing chamber
Step 3: wait for that standard flat light source completes preheating
Step 4: take each 1 minute 3～5 times
Step 5: the software on bioprotein chip reading instrument calculates the correction coefficient of 48 positions automatically
Step 6: take out standard flat light source, put into protection box, wait for that used next time
Known by above-mentioned example, while using LED standard flat light source provided by the invention to instrument calibration, uniformity coefficient that can calibration instrument, instrument, by taking the picture of standard flat light source, can calculate the uniformity coefficient of instrument entirety.
Also use LED standard flat light source provided by the invention to different instrument calibrations simultaneously, sensitization uniformity that can calibration instrument, by recording the measured value (the normally center of standard flat light source) of same position LED, calculate the measure error between any two instruments, by regulating the means such as aperture, make two instruments be less than 5% at this locational measurement error.
More than show and described basic principle of the present invention, principal character and advantage of the present invention.The technical staff of the industry should understand; the present invention is not restricted to the described embodiments; that in above-described embodiment and description, describes just illustrates principle of the present invention; without departing from the spirit and scope of the present invention; the present invention also has various changes and modifications, and these changes and improvements all fall in the claimed scope of the invention.The claimed scope of the present invention is defined by appending claims and equivalent thereof.
Claims (7)
1. the standard flat light source based on LED array, is characterized in that, described standard flat light source comprises:
Housing, described housing guarantees dull thread leakage;
LED light emitting array, described LED light emitting array is placed in housing;
LED brilliance control module, described LED brilliance control module is placed in housing, and controls to adjust the each LED in LED light emitting array, makes the LED brightness value worst error in LED light emitting array be less than 1%;
Interface module, described interface module is placed in housing, and joins with LED brilliance control module communication, realizes communicating by letter of LED brilliance control module and upper control system;
Power module, described power module is placed in housing, for the building block of standard flat light source provides stable operating voltage.
2. a kind of standard flat light source based on LED array according to claim 1, is characterized in that, described LED light emitting array is set to 8 × 6 arrays according to the form of protein chip.
3. a kind of standard flat light source based on LED array according to claim 1, it is characterized in that, described LED brilliance control module comprises CPU and LED brightness regulation chip, described CPU communicates by interface module and upper control system, and control connection LED brightness regulation chip, described LED brightness regulation chip controls connects the each LED in LED light emitting array.
4. a kind of standard flat light source based on LED array according to claim 3, is characterized in that, described LED brightness regulation chip is controlled the brightness of LED by current control mode and frequency control system.
5. a kind of standard flat light source based on LED array according to claim 4, is characterized in that, described current control mode is undertaken by following formula:
I
real=I
if× R/63
In formula: I
real-real work electric current; I
if-electric current; R-operating current arranges.
6. a kind of standard flat light source based on LED array according to claim 4, is characterized in that, described frequency control system is undertaken by following formula:
H
real=H
if× M/4095
In formula: H
real-actual operating frequency; H
if-setpoint frequency; M-operating frequency arranges.
7. a kind of standard flat light source based on LED array according to claim 1, it is characterized in that, described power module comprises lithium battery, power switch chip, voltage-stabilizing device and CPU, and the Voltage-output of described voltage-stabilizing device control lithium battery, guarantees the voltage that its stable output is consistent; The Voltage-output of described power switch chip controls lithium battery; Described CPU detects the output voltage of lithium battery in real time by A/D modular converter, the output voltage that lithium battery detected as CPU is when requiring, and standard of closure light source, has so just guaranteed that each start can obtain consistent operating voltage automatically; Described CPU detects that by time block the working time is more than or equal to the automatic shutdown time of setting, and just powered-down switch chip automatically, realizes automatic shutdown.
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