CN103773709A - Bacillus subtilis with efficient phosphate solubilizing effect and application thereof - Google Patents
Bacillus subtilis with efficient phosphate solubilizing effect and application thereof Download PDFInfo
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- 244000063299 Bacillus subtilis Species 0.000 title abstract description 12
- 235000014469 Bacillus subtilis Nutrition 0.000 title abstract description 11
- 229910019142 PO4 Inorganic materials 0.000 title abstract description 9
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 title abstract description 9
- 239000010452 phosphate Substances 0.000 title abstract description 9
- 230000003381 solubilizing effect Effects 0.000 title abstract description 4
- 235000015097 nutrients Nutrition 0.000 claims abstract description 7
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 claims description 30
- 229910052698 phosphorus Inorganic materials 0.000 claims description 30
- 239000011574 phosphorus Substances 0.000 claims description 30
- 241000894006 Bacteria Species 0.000 claims description 17
- 239000007788 liquid Substances 0.000 claims description 8
- 239000003795 chemical substances by application Substances 0.000 claims description 7
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- 238000009629 microbiological culture Methods 0.000 abstract 1
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- 108020004465 16S ribosomal RNA Proteins 0.000 description 19
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- 241000196324 Embryophyta Species 0.000 description 9
- 244000005700 microbiome Species 0.000 description 9
- 102000042567 non-coding RNA Human genes 0.000 description 7
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- 239000011780 sodium chloride Substances 0.000 description 5
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- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 4
- 238000006243 chemical reaction Methods 0.000 description 4
- 230000007928 solubilization Effects 0.000 description 4
- 238000005063 solubilization Methods 0.000 description 4
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- 241000194110 Bacillus sp. (in: Bacteria) Species 0.000 description 3
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- 229920002472 Starch Polymers 0.000 description 3
- 210000004027 cell Anatomy 0.000 description 3
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- 238000000034 method Methods 0.000 description 3
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- 239000002686 phosphate fertilizer Substances 0.000 description 3
- 238000011160 research Methods 0.000 description 3
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- 229920001817 Agar Polymers 0.000 description 2
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- 108010053835 Catalase Proteins 0.000 description 2
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- ZOKXTWBITQBERF-UHFFFAOYSA-N Molybdenum Chemical compound [Mo] ZOKXTWBITQBERF-UHFFFAOYSA-N 0.000 description 2
- 229910002651 NO3 Inorganic materials 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 2
- 239000008272 agar Substances 0.000 description 2
- -1 aluminium antimony Chemical compound 0.000 description 2
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 229940041514 candida albicans extract Drugs 0.000 description 2
- 239000003085 diluting agent Substances 0.000 description 2
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- 239000000203 mixture Substances 0.000 description 2
- AMWVZPDSWLOFKA-UHFFFAOYSA-N phosphanylidynemolybdenum Chemical compound [Mo]#P AMWVZPDSWLOFKA-UHFFFAOYSA-N 0.000 description 2
- 239000002367 phosphate rock Substances 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 238000006722 reduction reaction Methods 0.000 description 2
- 238000012216 screening Methods 0.000 description 2
- 238000012163 sequencing technique Methods 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 235000000346 sugar Nutrition 0.000 description 2
- 239000001038 titanium pigment Substances 0.000 description 2
- 230000017260 vegetative to reproductive phase transition of meristem Effects 0.000 description 2
- 239000012138 yeast extract Substances 0.000 description 2
- XMIIGOLPHOKFCH-UHFFFAOYSA-N 3-phenylpropionic acid Chemical compound OC(=O)CCC1=CC=CC=C1 XMIIGOLPHOKFCH-UHFFFAOYSA-N 0.000 description 1
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 1
- 241000563903 Bacillus velezensis Species 0.000 description 1
- 235000007516 Chrysanthemum Nutrition 0.000 description 1
- 244000189548 Chrysanthemum x morifolium Species 0.000 description 1
- KRKNYBCHXYNGOX-UHFFFAOYSA-K Citrate Chemical compound [O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O KRKNYBCHXYNGOX-UHFFFAOYSA-K 0.000 description 1
- 238000007400 DNA extraction Methods 0.000 description 1
- 239000001828 Gelatine Substances 0.000 description 1
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- NHNBFGGVMKEFGY-UHFFFAOYSA-N Nitrate Chemical compound [O-][N+]([O-])=O NHNBFGGVMKEFGY-UHFFFAOYSA-N 0.000 description 1
- 241000237502 Ostreidae Species 0.000 description 1
- 238000012408 PCR amplification Methods 0.000 description 1
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 1
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- CWHJIJJSDGEHNS-MYLFLSLOSA-N Senegenin Chemical compound C1[C@H](O)[C@H](O)[C@@](C)(C(O)=O)[C@@H]2CC[C@@]3(C)C(CC[C@]4(CCC(C[C@H]44)(C)C)C(O)=O)=C4[C@@H](CCl)C[C@@H]3[C@]21C CWHJIJJSDGEHNS-MYLFLSLOSA-N 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- DPDMMXDBJGCCQC-UHFFFAOYSA-N [Na].[Cl] Chemical compound [Na].[Cl] DPDMMXDBJGCCQC-UHFFFAOYSA-N 0.000 description 1
- DYJGATXRPZAVGS-UHFFFAOYSA-N [Sc].[Sb].[Mo] Chemical compound [Sc].[Sb].[Mo] DYJGATXRPZAVGS-UHFFFAOYSA-N 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- APUPEJJSWDHEBO-UHFFFAOYSA-P ammonium molybdate Chemical compound [NH4+].[NH4+].[O-][Mo]([O-])(=O)=O APUPEJJSWDHEBO-UHFFFAOYSA-P 0.000 description 1
- 235000018660 ammonium molybdate Nutrition 0.000 description 1
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- 230000009604 anaerobic growth Effects 0.000 description 1
- 230000003042 antagnostic effect Effects 0.000 description 1
- AUJJPYKPIQVRDH-UHFFFAOYSA-N antimony potassium Chemical compound [K].[Sb] AUJJPYKPIQVRDH-UHFFFAOYSA-N 0.000 description 1
- 238000010876 biochemical test Methods 0.000 description 1
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- ATNHDLDRLWWWCB-AENOIHSZSA-M chlorophyll a Chemical compound C1([C@@H](C(=O)OC)C(=O)C2=C3C)=C2N2C3=CC(C(CC)=C3C)=[N+]4C3=CC3=C(C=C)C(C)=C5N3[Mg-2]42[N+]2=C1[C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@H](C)C2=C5 ATNHDLDRLWWWCB-AENOIHSZSA-M 0.000 description 1
- 230000004087 circulation Effects 0.000 description 1
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- 229930182470 glycoside Natural products 0.000 description 1
- 150000002338 glycosides Chemical class 0.000 description 1
- 239000011964 heteropoly acid Substances 0.000 description 1
- BHEPBYXIRTUNPN-UHFFFAOYSA-N hydridophosphorus(.) (triplet) Chemical compound [PH] BHEPBYXIRTUNPN-UHFFFAOYSA-N 0.000 description 1
- 238000009654 indole test Methods 0.000 description 1
- 230000001788 irregular Effects 0.000 description 1
- 235000021374 legumes Nutrition 0.000 description 1
- 230000031700 light absorption Effects 0.000 description 1
- 230000002906 microbiologic effect Effects 0.000 description 1
- VLAPMBHFAWRUQP-UHFFFAOYSA-L molybdic acid Chemical compound O[Mo](O)(=O)=O VLAPMBHFAWRUQP-UHFFFAOYSA-L 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 235000020636 oyster Nutrition 0.000 description 1
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- 239000009871 tenuigenin Substances 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- OBSZRRSYVTXPNB-UHFFFAOYSA-N tetraphosphorus Chemical compound P12P3P1P32 OBSZRRSYVTXPNB-UHFFFAOYSA-N 0.000 description 1
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- 108010046845 tryptones Proteins 0.000 description 1
- 229910021642 ultra pure water Inorganic materials 0.000 description 1
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- 239000001052 yellow pigment Substances 0.000 description 1
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention relates to a high-efficiency phosphate solubilizing bacterium bacillus subtilis. The bacillus subtilis is preserved in the China general microbiological culture Collection center with the preservation number of CGMCC No.8241, has higher phosphate-solubilizing effect, does not pollute the environment and is ecologically safe. The high-efficiency bacillus subtilis is used alone or together with other organic and inorganic nutrients required by plant growth to prepare a biological compound fertilizer with the functions of improving yield and improving quality.
Description
Technical field
The present invention relates to microorganism and biological fertilizer field, particularly relate to subtilis, particularly, the present invention relates to a kind of subtilis and application thereof of efficient phosphate-solubilizing effect.
Background technology
Phosphate fertilizer can promote plant bud differentiation, and early flowering result promotes seedlings root growth and improves fruit quality.Execute phosphorus and can promote various Metabolism of Normals to carry out, growth and development of plants is good, improves winter resistance and the drought resistance of plant simultaneously.Because phosphorus and metabolism and three's phase co-conversion of carbohydrate, protein and fat have relation, no matter all need phosphate fertilizer mainly with cultivation food crop, legume crop and oil crop.While lacking phosphorus, protein synthesis is obstructed, and new tenuigenin and nucleus form less, affect cell fission, and poor growth is also few, branch or the minimizing of tillering, and plant is short and small, and blade is dark green, may be that Growth of Cells is slow, and chlorophyll content improves relatively.Certain plants leaf takes on a red color or purple sometimes.Hinder sugar transportation because lack phosphorus, also oneself tired out a large amount of sugars, be conducive to the formation of yellow pigment glycosides.While lacking phosphorus, flowering period and ripening stage all postpone, yield reducation, and resistance weakens.But there is 74% the scarce phosphorus of arable soil in China, phosphorus in soil more than 95% is invalid form, plant is difficult to directly absorb, and in addition, uses in a large number chemical fertilizer obviously to cause the adverse consequencess such as soil compaction, the decline of soil water-retaining power, grassland degeneration, desertification be serious.Therefore, the utilization ratio of raising phosphorus is the focus that agronomy, soil science, ecotope and microbiological research person pay close attention to always.
People start to notice the relation between microorganism and soil phosphorus in 20 beginnings of the century.During the mixture of Sackett(1908) finding some insolublies is manured into soil, can be used as phosphorus source and applies, they filter out 50 strain bacteriums from soil, and wherein 36 strains have formed macroscopic molten phosphorus circle on flat board.Gerretsen in 1948 finds that plant applies insoluble phosphate fertilizer, after inoculation soil microorganisms, has promoted the growth of plant, increases the absorption of phosphorus.He has isolated these microorganisms, finds that these microorganisms can help the dissolving of ground phosphate rock.From then on, many scientists are devoted to the research of phosphate solubilizing bacteria, have in succession reported that many microorganisms have phosphate solubilization.
Present inventor, through concentrated research for many years, is separated to the new bacillus subtilis strain of a strain from plant rhizosphere soil, and this bacterial strain has stronger phosphate solubilization, invalid phosphorus effectively can be transformed into available phosphorus.
Summary of the invention
The object of the present invention is to provide the bacterial strain of a kind of new subtilis, described bacterial strain has good phosphate solubilization.
Subtilis of the present invention (Bacillus subtilis) has been deposited in China Committee for Culture Collection of Microorganisms's common micro-organisms center on September 24th, 2013, depositary institution address is No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, and deposit number is CGMCC No.8241.
Further, the invention provides the purposes of above-mentioned subtilis aspect phosphorus decomposing.
Further, subtilis of the present invention can be made microbiobacterial agent, cultivates in LB nutrient solution by described bacterial strain, and culture temperature is 27 ℃, and cultivated days is 5 days, and rotating speed is 200 rpm, and nutrient solution is adjusted to 10 by bacterium liquid cell count after filtering
9individual/mL, makes subtilis microbiobacterial agent of the present invention.
Subtilis provided by the present invention separates and obtains from plant rhizosphere soil, its laboratory called after bacterial strain Fdp6.
Bacterial strain Fdp6 is rounded at the upper bacterium colony of LB solid medium (substratum consists of: Tryptones 10g/L, yeast extract 5g/L, sodium-chlor 10g/L, agar 15g/L), and edge is irregular, and oyster white is opaque, and surface irregularity has fold, tarnish.Be cultured to 30h, thalline all exists with the form of hypopus gemma.It is light yellow that bacterium colony is.Microscopic examination thalline is unicellular, shaft-like, single raw or twin, sometimes becomes chain.Gram-positive, gemma ellipse.VP reaction, Starch Hydrolysis test, nitrate reduction reaction, Citrate trianion utilization, gelatine liquefication are all positive; The utilization of the test of phenylpropionic acid desaminase, M.R., urine enzyme test, propionic salt is all negative, can be containing growing in the LB substratum of 6.5% NaCl.Utilize 16S rDNA gene specific primer to increase to 16S rDNA, amplified production is reclaimed and check order, sequencing result is carried out to Blast analysis, found that with the bacterial strain of bacterial strain Fdp6 height homology are all genus bacillus, homology is more than 98%, and in conjunction with physiological and biochemical property, identify that it is subtilis.
Effect of the present invention
In flat band method, cultivate on the solid medium that contains insoluble phosphate or organophosphorus by phosphate solubilizing bacteria, periphery of bacterial colonies produces larger molten phosphorus circle, it is 400.236 mg/L that subtilis of the present invention records its phosphorus decomposing effect through phosphorus molybdenum blue spectrophotometric method, illustrates that bacterial strain of the present invention has stronger phosphate solubilization.
Embodiment
The invention discloses a kind of efficient phosphate-solubilizing bacterium subtilis, those skilled in the art can use for reference content herein, suitably improve processing parameter and realize.Special needs to be pointed out is, all similar replacements and change apparent to those skilled in the art, these be all deemed to be included in by invention in.Subtilis of the present invention is described by the embodiment compared with standard, related personnel obviously can change methods and applications as herein described and suitable change and combination not breaking away from content of the present invention, spirit and scope, realizes and apply the technology of the present invention.
Below in conjunction with embodiment, further set forth the present invention.
the separation of embodiment 1 microorganism from soil sample
From near the Huang top chrysanthemum rhizosphere soil of adopting back the Hengshui Lake of Hebei, take 10g soil 90mL sterilized water and mix, then dilute therefrom getting 1mL sample 9mL sterilized water, carry out 6 times with this, be diluted to 10
-6, 10
-7, 10
-83 gradients, pipette bacteria suspension 100 microlitres of last three gradients, coat Meng Jinna inorganic phosphorus substratum (glucose 10 g, (NH
4)
2sO4 0.5 g, NaCl 0.3 g, KCl 0.3 g, FeSO47H2O 0.03 g, MnSO44H2O 0.03 g, MgSO47H2O 0.3 g, ground phosphate rock 10 g, yeast extract paste 0.4 g, agar 15 g, distilled water 1000 ml, pH 7.0~7.5) on flat board, with last diluent in contrast, at 28 ℃, cultivate, and every 24h observes once.Select in contrast without bacterium colony, and in diluent before this, grow the bacterial strain of bacterium colony, jump and get single bacterium colony and cultivate in liquid Meng Jinna inorganic phosphorus substratum according to the feature such as colonial morphology, color, and purifying numbering.
embodiment 2phosphorus decomposing
the screening of bacterial strain
Under gnotobasis, all inoculation, to Meng Jinna substratum, are cultivated 7 days for 30 ℃.Observe colony growth situation in flat board, filter out efficient phosphate-solubilizing bacterium according to producing phosphorus decomposing circle size.
the sequencing of the 16S rDNA of embodiment 3 bacterial strains and analysis and physiological and biochemical test are identified
Subtilis DNA extraction adopts conventional phenol method, and by bacterium universal primer amplification 16S rDNA sequence, primer sequence is 27F (AGAGTTTGATCCTGGCTCAG) and 1492R (GGTTACCTTGTTACGACTT).PCR reaction system (50 μ L) is: 10 X PCR damping fluid 5 μ L, dNTP 4 μ L, the each 1 μ L of primer, DNA profiling 2.5 μ L, Takara Taq enzyme 0.25 μ L, ultrapure water 36 μ L.Pcr amplification program is 94 ℃ of 3min; 94 ℃ of 1min, 52 ℃ of 1 min, 72 ℃ of 1.5 min, 30 circulations; 72 ℃ of 10 min.Amplified production is served Hai Sheng work bio tech ltd and is checked order.
By the 16S rDNA sequence input GenBank recording, application BLAST software carries out homology search.Choose the 16S rDNA sequence of different strains and with GenBank in known 16S rDNA carry out homology comparison.
Homology comparative result is as follows:
This pcr amplification product is carried out in GenBank to blast comparison result and show, 50 bacterial classifications of bacterial strain Fdp6 of the present invention and its height homology are all bacillus, and its homology is all more than 99%.Table 1 is depicted as 10 high bacterial strains of 16S rDNA homology of bacterial strain Fdp6.Bacterial strain physiological and biochemical index deducibility Fdp6 bacterial strain shown in associative list 2 belongs to subtilis thus.
The comparison of table 1 16S rDNA homology
Serial ID | Bacterial strain | Homology |
AB740156.1 | Bacillus subtilis gene for 16S rRNA, partial sequence, strain: Szi4-15 | 100% |
KC121034.1 | Bacillus sp. JN08 16S ribosomal RNA gene, partial sequence | 100% |
JX390617.1 | Bacillus subtilis strain NEHU.FNSRJ.113 16S ribosomal RNA gene, partial sequence | 100% |
JQ267474.1 | Bacillus subtilis strain LY-001 16S ribosomal RNA gene, partial sequence >gb|JX849029.1| Bacillus sp. M7(2012) 16S ribosomal RNA gene, partial sequence | 100% |
JQ023605.1 | Bacillus methylotrophicus strain LZ043 16S ribosomal RNA gene, partial sequence | 100% |
JN400257.1 | Bacillus subtilis strain WRL-101 16S ribosomal RNA gene, partial sequence | 100% |
EU729126.1 | Bacillus subtilis strain JSU-2 16S ribosomal RNA gene, partial sequence | 100% |
AM981259.1 | Bacillus sp. NIOT-2 partial 16S rRNA gene, isolate NIOT-2 | 100% |
AB848923.1 | Bacillus subtilis gene for 16S ribosomal RNA, partial sequence | 99% |
The antagonistic bacterium of screening is carried out to preliminary Physiology and biochemistry to be identified, comprise gram mensuration, catalase reaction, VP test, anaerobic growth, Starch Hydrolysis, 50 ℃ of growths and 6.5% NaCl growth test, described test method adopts the conventional methods of this class physiological and biochemical index of mensuration of this area.
The Determination of Physiological And Biochemical Indices of table 2 bacterial strain
Testing index | Strain characteristics | Testing index | Strain characteristics |
Gramstaining test | + | Indole test | - |
MR test | + | VP test | - |
Catalase test | + | H 2S produces | - |
Nitrate reduction test | - | Sugar alcohol fermentation test | - |
6.5% NaCl | + | Hydrolyzed starch test | - |
The fermentation test of glucose glycosyloxy | - |
the preparation of embodiment 4 subtilis microbiobacterial agent of the present invention
The subtilis that by deposit number is CGMCC No. 8241 is cultivated in LB nutrient solution, and culture-liquid temp is 27 ℃, and number of days is 5 days, and rotating speed is 200rpm, and pH value is 8.After fermentation culture, add 5% sucrose, then filtering and regulating bacterium liquid cell count is 10
9individual/mL, makes subtilis microbiobacterial agent of the present invention.
embodiment 5 molybdenum antimony scandium colorimetric method for determining microorganism phosphorus decomposing abilities
In phosphorous solution, add ammonium molybdate, under certain acidity condition, phosphoric acid in solution and molybdic acid complexing form yellow phosphorus molybdenum heterozygosis acid one phosphorus molybdenum Huang, then make reductive agent with xitix tartarize antimony potassium, can make phosphato-molybdic heteropolyacid change into stable blue solution (molybdenum blue), its shade and phosphorus content are proportional, can carry out thus colorimetric assay. measure the light absorption value of molybdenum blue at wavelength 700 nm places with spectrophotometer, with quantitative analysis phosphorus content.
The drafting of phosphorus typical curve
Draw successively 0.0,0.8,1.6,2.4,3.2,4.0 mL 5 mg/L phosphoric acid standardized solution and enter test tube, respectively add the anti-developer of 2 mL aluminium antimony, be settled to 20 mL with distilled water, shake up and leave standstill after 15-20 min, under 700 nm, measure absorbancy and " take absorbancy as ordinate zou; take phosphorus concentration as X-coordinate, draw phosphorus typical curve ", now in each pipe, phosphorus concentration is respectively: 0.00,0.20,0.40,0.60,0.80,1.00 mg/L.
The mensuration of titanium pigment content in nutrient solution
Get 1 mL at insoluble inorganic phosphorus liquid nutrient medium (glucose 10 g, (NH
4)
2sO
40.5 g, NaCl 0.3 g, KCl 0.3 g, MgSO
47H
2o 0.3 g, FeSO
47H
2o 0.03 g, MnSO
4h
2o 0.03 g, Ca
3(PO
4)
25.0 g, be settled to 1000 mL, pH 7.0~7.5) in cultivate thalline fermented liquid after 4d with centrifugal 10 min of 10000 g, supernatant liquor adds absorption 200 μ L and enters color board after suitably diluting, add again anti-developer 2 mL of aluminium antimony, distilled water is settled to 20 mL, leaves standstill after 15-20 min, and under 700 nm, measuring titanium pigment content is 400.236 mg/L.
A kind of efficient phosphate-solubilizing bacterium subtilis of the present invention and application thereof are described by concrete example, those skilled in the art can use for reference content of the present invention, the links such as appropriate change raw material, processing condition realize corresponding other object, its relevant change does not all depart from content of the present invention, within all similar replacements and change will become apparent to those skilled in the art that and be all deemed to be included in scope of the present invention.
Claims (3)
1. a subtilis, is characterized in that, deposit number is CGMCC No. 8241.
2. the application of the subtilis that a deposit number is CGMCC No.8241 aspect phosphorus decomposing.
3. a microbiobacterial agent, is characterized in that, subtilis claimed in claim 1 is cultivated in LB nutrient solution, and culture-liquid temp is 27 ℃, and number of days is 5 days, and rotating speed is 200 rpm, and then filtering and regulating bacterium liquid cell count is 10
9individual/mL, makes microbiobacterial agent.
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Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
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CN104195078A (en) * | 2014-08-22 | 2014-12-10 | 西安文理学院 | Bacillus subtilis |
CN104263679A (en) * | 2014-09-03 | 2015-01-07 | 南京聚肽高科农业有限公司 | High-efficiency phosphate-solubilizing bacteria and application thereof |
CN110591941A (en) * | 2019-08-29 | 2019-12-20 | 甘肃省科学院生物研究所 | Bacillus subtilis with efficient degradation effect on organic phosphorus and preparation method thereof |
CN112625947A (en) * | 2020-12-16 | 2021-04-09 | 江苏省中国科学院植物研究所 | Bacillus subtilis capable of dissolving phosphorus strongly, carbon-based microbial compound fertilizer thereof and application of bacillus subtilis |
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CN101899405A (en) * | 2010-01-20 | 2010-12-01 | 辛伟成 | Bacillus subtilis GU and application in preparing efficient biological compound phosphate fertilizer thereof |
CN102061273A (en) * | 2010-11-05 | 2011-05-18 | 南开大学 | Bacillus subtilis with phosphate-solubilizing effect and application thereof |
CN102399713A (en) * | 2011-09-22 | 2012-04-04 | 华南农业大学 | Bacillus subtilis HL-1 and application thereof in respect of soil phosphate dissolving |
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CN101899405A (en) * | 2010-01-20 | 2010-12-01 | 辛伟成 | Bacillus subtilis GU and application in preparing efficient biological compound phosphate fertilizer thereof |
CN102061273A (en) * | 2010-11-05 | 2011-05-18 | 南开大学 | Bacillus subtilis with phosphate-solubilizing effect and application thereof |
CN102399713A (en) * | 2011-09-22 | 2012-04-04 | 华南农业大学 | Bacillus subtilis HL-1 and application thereof in respect of soil phosphate dissolving |
Cited By (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104195078A (en) * | 2014-08-22 | 2014-12-10 | 西安文理学院 | Bacillus subtilis |
CN104195078B (en) * | 2014-08-22 | 2017-01-11 | 西安文理学院 | Bacillus subtilis |
CN104263679A (en) * | 2014-09-03 | 2015-01-07 | 南京聚肽高科农业有限公司 | High-efficiency phosphate-solubilizing bacteria and application thereof |
CN110591941A (en) * | 2019-08-29 | 2019-12-20 | 甘肃省科学院生物研究所 | Bacillus subtilis with efficient degradation effect on organic phosphorus and preparation method thereof |
CN112625947A (en) * | 2020-12-16 | 2021-04-09 | 江苏省中国科学院植物研究所 | Bacillus subtilis capable of dissolving phosphorus strongly, carbon-based microbial compound fertilizer thereof and application of bacillus subtilis |
CN112625947B (en) * | 2020-12-16 | 2021-08-03 | 江苏省中国科学院植物研究所 | Bacillus subtilis capable of dissolving phosphorus strongly, carbon-based microbial compound fertilizer thereof and application of bacillus subtilis |
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