CN103773671B - Cell counting chamber, mould and method for manufacturing cell counting chamber - Google Patents
Cell counting chamber, mould and method for manufacturing cell counting chamber Download PDFInfo
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- CN103773671B CN103773671B CN201410042530.2A CN201410042530A CN103773671B CN 103773671 B CN103773671 B CN 103773671B CN 201410042530 A CN201410042530 A CN 201410042530A CN 103773671 B CN103773671 B CN 103773671B
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/36—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of biomass, e.g. colony counters or by turbidity measurements
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B29—WORKING OF PLASTICS; WORKING OF SUBSTANCES IN A PLASTIC STATE IN GENERAL
- B29C—SHAPING OR JOINING OF PLASTICS; SHAPING OF MATERIAL IN A PLASTIC STATE, NOT OTHERWISE PROVIDED FOR; AFTER-TREATMENT OF THE SHAPED PRODUCTS, e.g. REPAIRING
- B29C45/00—Injection moulding, i.e. forcing the required volume of moulding material through a nozzle into a closed mould; Apparatus therefor
- B29C45/0025—Preventing defects on the moulded article, e.g. weld lines, shrinkage marks
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B29—WORKING OF PLASTICS; WORKING OF SUBSTANCES IN A PLASTIC STATE IN GENERAL
- B29C—SHAPING OR JOINING OF PLASTICS; SHAPING OF MATERIAL IN A PLASTIC STATE, NOT OTHERWISE PROVIDED FOR; AFTER-TREATMENT OF THE SHAPED PRODUCTS, e.g. REPAIRING
- B29C45/00—Injection moulding, i.e. forcing the required volume of moulding material through a nozzle into a closed mould; Apparatus therefor
- B29C45/17—Component parts, details or accessories; Auxiliary operations
- B29C45/26—Moulds
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Abstract
The invention provides a cell counting chamber comprising a box body and a first substrate, wherein the box body comprises a second substrate and a wall arranged around the second substrate; a boss is formed on the inner side of the upper edge wall of the second substrate; anti-shrinkage grooves are formed on the second substrate; the anti-shrinkage grooves are arranged on an inner surface of the second substrate formed with the boss and an outer surface opposite to the inner surface, and the anti-shrinkage groove on the inner surface is close to the boss; the first substrate is embedded in the box body, and forms a counting chamber cavity together with the second substrate and the boss; and an opening structure which is communicated with the counting chamber cavity and is used for loading a sample to the chamber is formed on the counting chamber cavity. The cell counting chamber disclosed by the invention, through the anti-shrinkage grooves, can reduce shrinkage deformation of a middle part which is relatively low in thickness, so that the inner surface and the outer surface are smoother. Furthermore, the invention provides a mould for injection-moulding the cell counting chamber, and a method for manufacturing the cell counting chamber through the mould.
Description
Technical field
The present invention relates to cytometry field, more particularly, relate to a kind of cell counting count board, and for the mould of this cell counting count board of injection moulding, and a kind of method utilizing above-mentioned Making mold cell counting count board.
Background technology
In biological study, sometimes need to calculate the cell quantity in unit volume, in existing counting, in this process, the general cell counting count board that adopts is calculated cell quantity.
Existing cell counting count board is made up of box body and the substrate be arranged in box body dented space, wherein there is gap between the bottom surface of the dented space of box body and substrate, this gap space is the counting cell cavity of cell counting count board, sample liquid is introduced in this counting cell cavity, just can count it.
Owing to needing to adopt microscope to observe the sample in cell counting count board, so the box body of composition cell counting count board and substrate need to adopt transparent material manufacture to form, in the production process of reality, the manufactured materials of box body and substrate generally selects glass or clear plastics.When employing clear plastics, such as PVC is as manufactured materials and when utilizing mould one-body molded, because clear plastics self has easy shrinkability, so transparent plastic material in a mold cooling forming time, its surface is easy to shrink.And the effect of cell counting count board is the cell of support personnel to small volume counts, so the flatness requirement of the manufacturing process of cell counting count board to box body and substrate is higher, and the contraction that the box body of clear plastics material and substrate occur in the fabrication process can cause larger impact to the planeness on its surface, microscope is focused on not accurate enough, have impact on the result of use of cell counting count board.
Therefore, how improving the result of use of cell counting count board, is current those skilled in the art's problem demanding prompt solution.
Summary of the invention
In view of this, the invention provides the cell counting count board that a kind of planeness is higher, it can improve microscopical focusing accuracy, further increases the result of use of self.
In order to achieve the above object, the invention provides following technical scheme:
A kind of cell counting count board, it comprises: box body and first substrate, wherein:
Described box body comprises: second substrate and the wall be arranged at around described second substrate, described second substrate is provided with boss inside described wall;
Described second substrate is provided with anti-shrinkage groove, and described anti-shrinkage groove is arranged on the internal surface that described second substrate is provided with boss, and on the outside surface relative with described internal surface, and the described anti-shrinkage groove be positioned on described internal surface is arranged along described boss;
Described first substrate is embedded in described box body, and forms counting cell cavity with described second substrate, described boss;
Described counting cell cavity offers the hatch frame filling pond for application of sample be communicated with described counting cell cavity.
Preferably, in above-mentioned cell counting count board, described box body is hamper, and described first substrate is base plate, and described second substrate is cover plate.
Preferably, in above-mentioned cell counting count board, described anti-shrinkage groove is in the frame-shaped vibrational power flow with opening on the internal surface of described cover plate, and the described anti-shrinkage groove be positioned on described outside surface is arranged in the same fashion; Described second substrate offers described hatch frame, and described hatch frame is positioned at the opening of described anti-shrinkage groove.
Preferably, in above-mentioned cell counting count board, described box body is end box, and described first substrate is cover plate, and described second substrate is base plate.
Preferably, in above-mentioned cell counting count board, described anti-shrinkage groove is closed frame-shaped vibrational power flow on the internal surface of described base plate, and the described anti-shrinkage groove be positioned on described outside surface is arranged in the same fashion; Described hatch frame is arranged on the edge of described cover plate side, and the position of described anti-shrinkage groove on described hatch frame and described base plate is oppositely arranged.
A kind of mould, it is for the manufacture of the box body of cell counting count board described above, comprises upper bolster and hamper die shoe, wherein:
Described upper bolster is the stepped flange in bottom surface, the bottom surface of the convex part of described flange is provided with the upper raised line protruding described bottom surface;
The hamper die shoe coordinated with described upper bolster, the end face of described hamper die shoe is provided with the projection that can form described hatch frame on described box body; The edge of described hamper die shoe is provided with the frame protruding described die shoe end face, described frame offers the opening for feed running through described frame in the horizontal direction; The end face of described hamper die shoe is provided with the lower raised line protruding described end face.
A kind of mould, it is for the manufacture of the box body of cell counting count board described above, comprises upper bolster and end box die shoe, wherein:
Described upper bolster is the stepped flange in bottom surface, the bottom surface of the convex part of described flange is provided with the upper raised line protruding described bottom surface;
The end box die shoe coordinated with described upper bolster, the end face of box die shoe of the described end is provided with the lower raised line protruding described end face, the edge of box die shoe of the described end arranges the frame protruding box die shoe end face of the described end, described frame offers the opening for feed running through described frame in the horizontal direction.
Manufacture a method for cell counting count board, the box body of the cell counting count board that the method utilizes Making mold described above described above, it comprises the following steps:
1) described upper bolster is fastened on die shoe;
2) in the internal space that described upper bolster and the cooperation of described die shoe are formed, injectable plastic material is injected by described opening for feed;
3) make the injectable plastic material cooling forming entered in described internal space, obtain described box body;
4) injection moulding forms first substrate, and assembles with described box body, obtains complete cell counting count board.
In cell counting count board provided by the invention, it comprises box body and first substrate, and this box body comprises second substrate and is arranged on the wall around substrate, and at wall, the inner side at second substrate center is provided with boss.First substrate can embed in box body, by wall and its clamping, and coordinates with boss and contacts, and common under the cooperation of second substrate form can the counting cell cavity of storing sample.Counting cell cavity offers the hatch frame be communicated with it, for adding sample in counting cell cavity.And on the chamber wall of second substrate, be provided with anti-shrinkage groove, box body is in the process of cooling forming, edge due to second substrate is provided with the structure such as wall and boss, make the integral thickness of its edge larger, and then cause the shrinkage of this part also larger, and anti-shrinkage groove is set on internal surface and outside surface, the impact that the larger contraction deformation quantity that farthest can reduce edge causes the internal surface of second substrate middle portion and the planeness of outside surface, the contraction deformation quantity at edge is avoided to affect the surface of second substrate middle portion that larger shrinkage strain occurs, ensure that internal surface and outside surface can be more smooth.Cell counting count board provided by the invention, by arranging anti-shrinkage groove, to reduce second substrate edge deformation amount to the impact of middle portion, thus the contraction deformation quantity that the middle portion making thickness relatively thin occurs is less, make internal surface and outside surface more smooth, microscopical focusing accuracy can be improved, further increase the result of use of cell counting count board.Present invention also offers a kind of mould for this cell counting count board of injection moulding, and a kind of method utilizing above-mentioned Making mold cell counting count board.
Accompanying drawing explanation
In order to be illustrated more clearly in the embodiment of the present invention or technical scheme of the prior art, be briefly described to the accompanying drawing used required in embodiment or description of the prior art below, apparently, accompanying drawing in the following describes is only some embodiments of the present invention, for those of ordinary skill in the art, under the prerequisite not paying creative work, other accompanying drawing can also be obtained according to these accompanying drawings.
The main pseudosection (box body is hamper) of the cell counting count board that Fig. 1 provides for a kind of specific embodiment of the present invention;
Fig. 2 is the main pseudosection of box body in Fig. 1;
Fig. 3 is the upward view of box body shown in Fig. 1;
The upward view of Fig. 4 to be hatch frame be box body of another kind of shape;
The main pseudosection (box body is end box) of the cell counting count board that Fig. 5 provides for another embodiment of the present invention;
Fig. 6 is the main pseudosection of box body in Fig. 5;
Fig. 7 is the upward view of box body shown in Fig. 6;
Fig. 8 is the structural representation of first substrate in a kind of embodiment;
Fig. 9 is the structural representation of first substrate in another embodiment;
Figure 10 is the schematic diagram of the base plate with focusing mark;
Figure 11 is the schematic diagram of the base plate with counting grid;
Figure 12 is the sectional view of upper bolster;
Figure 13 is the sectional view of end box die shoe;
Figure 14 is the schematic diagram that upper bolster and end box die shoe coordinate.
In above Fig. 1-Figure 14:
First substrate 1, second substrate 2, wall 3, boss 4, anti-shrinkage groove 5, hatch frame 6, upper bolster 7, end box die shoe 8, upper raised line 9, frame 10, opening for feed 11, lower raised line 12, arc notch 13, internal surface 14, outside surface 15, focusing mark 16, counting grid 17.
Embodiment
The invention provides a kind of cell counting count board, it can improve microscopical focusing accuracy, further increases the result of use of self.
Below in conjunction with the accompanying drawing in the embodiment of the present invention, be clearly and completely described the technical scheme in the embodiment of the present invention, obviously, described embodiment is only the present invention's part embodiment, instead of whole embodiments.Based on the embodiment in the present invention, those of ordinary skill in the art, not making the every other embodiment obtained under creative work prerequisite, belong to the scope of protection of the invention.
As Figure 1-Figure 11, the cell counting count board that the embodiment of the present invention provides, it comprises: box body and first substrate 1, wherein:
Box body comprises: second substrate 2 and the wall 3 be arranged at around second substrate 2, be provided with boss 4 on second substrate 2 inside wall 3;
Be provided with anti-shrinkage groove 5 on second substrate 2, and anti-shrinkage groove 5 is arranged on the internal surface 14 that second substrate 2 is provided with boss 4, and on the outside surface 15 relative with internal surface 14, and the anti-shrinkage groove 5 be positioned on internal surface 14 is arranged along boss 4;
First substrate 1 is embedded in described box body, and forms counting cell cavity with second substrate 2, boss 4;
Counting cell cavity offers the hatch frame 6 filling pond for application of sample be communicated with counting cell cavity.
In the cell counting count board that the present embodiment provides, it comprises box body and first substrate 1, and this box body comprises second substrate 2 and is arranged on the wall 3 around substrate, and at wall 3, the inner side at second substrate 2 center is provided with boss 4.First substrate 1 can embed in box body, is clamped by wall 3, and coordinates with boss 4 and contact, and common under the cooperation of second substrate 2 form can the counting cell cavity of storing sample.Counting cell cavity offers the hatch frame 6 be communicated with it, for adding sample in counting cell cavity.And on the chamber wall 3 of second substrate 2, be provided with anti-shrinkage groove 5, box body is in the process of cooling forming, edge due to second substrate 2 is provided with the structure such as wall 3 and boss 4, make the integral thickness of its edge larger, and then cause the shrinkage of this part also larger, and anti-shrinkage groove 5 is set on internal surface 14 and outside surface 15, the impact that the larger contraction deformation quantity that farthest can reduce edge causes the internal surface 14 of second substrate 2 middle portion and the planeness of outside surface 15, the contraction deformation quantity at edge is avoided to affect the surface of second substrate 2 middle portion that larger shrinkage strain occurs, ensure that internal surface 14 and outside surface 15 can be more smooth.
The cell counting count board that the present embodiment provides, by arranging anti-shrinkage groove 5, to reduce second substrate 2 edge deformation amount to the impact of middle portion, thus the contraction deformation quantity that the middle portion making thickness relatively thin occurs is less, make internal surface 14 and outside surface 15 more smooth, microscopical focusing accuracy can be improved, further increase the result of use of cell counting count board.
In said structure, the edge of second substrate 2 is provided with wall 3, as depicted in figs. 1 and 2.In the present embodiment, the box body that first substrate 1 is directly embedded in uncovered is interior to be tightly connected with boss 4, and the edge of first substrate 1 and wall 3 clamp, when first substrate 1 is embedding move to contact with boss 4 time, boss 4 pairs of first substrates 1 play and stop location and supporting role, the space now formed between first substrate 1 and box body is counting cell cavity, wherein, the thickness of boss 4 determines the height of counting cell cavity, the thickness of boss 4 is determined by species of samples to be measured, the interior empty shape of boss 4 determines the shape of counting cell cavity, it can be rectangle, circular, oval etc.That is, first substrate 1 realizes and being tightly connected of box body by clamping with wall 3, thus forms the counting cell cavity of no leakage (gas).Certainly, first substrate 1 and being tightly connected of box body also can realize by other means, such as, make first substrate 1 and boss 4 directly seal bonding.Wherein, box body also can be without wall 3 structure, namely only arranges boss 4 on second substrate 2 using as the structure be connected with first substrate 1, but the preferred second substrate 2 of the present embodiment is provided with wall 3, to be better tightly connected with first substrate 1 and to coordinate.
The preferred counting cell cavity of the present embodiment is cuboid, and namely the shape of cross section of counting cell cavity is rectangle.Certainly, under the prerequisite that the cell counting count board not affecting the present embodiment and provide normally works, the shape of cross section of counting cell cavity can also be other shape such as circular, oval.
In order to optimize technique scheme further, above-mentioned hatch frame 6 is the passage being communicated with counting cell cavity and outside air, its concrete set-up mode and structure can be multiple different form, when box body is hamper, such as shown in Fig. 3 and Fig. 4, hatch frame 6 is for running through the passage of second substrate 2, and its cross-sectional shape is rectangle or is similar to spherical dry tubulose.When box body is end box, hatch frame 6 also for directly to dig out an arc groove on a side of first substrate 1, or can dig arc groove after first substrate 1 is cut away one piece again, as shown in Figure 8 and Figure 9; And correspondingly on the boss of end box dig out an arc groove, as shown in Fig. 5, Fig. 6, Fig. 7.Certainly, hatch frame 6 also can be set to the multiple through holes running through first substrate 1 or second substrate 2, and comprising well and venting hole, venting hole is for discharging the air in counting cell cavity, and well is used for adding liquid sample in counting cell cavity.
When hatch frame 6 is as well and venting hole mode, the opening part that well is positioned at counting cell box body outer side is provided with overflow-proof ring.This overflow-proof ring is arranged on the well opening part of box body outer side, and overflow-proof ring forms the trumpet-shaped inlet of well, and when avoiding application of sample, liquid leakage causes polluting.
Than the above described structure, hatch frame 6 can also be set to the form of lateral openings, its implementation is on three edges being arranged in second substrate 2 four circumferential edge of boss 4 and wall 3, does not arrange the edge of boss 4 and wall 3 as hatch frame 6.Be about to be arranged on boss 4 on second substrate 2 one lateral edges and wall 3 directly removes, make first substrate 1 with box body aggregate erection after, form an opening of counting cell cavity in the side not arranging boss 4 and wall 3, thus operator can be made directly to be put in counting cell cavity by sample to be measured in this opening.
In a kind of specific embodiment, box body is the hamper for being covered in top, and first substrate 1 is for being positioned at the base plate bottom hamper, and second substrate 2 is cover plate, as depicted in figs. 1 and 2.
When first substrate 1 is base plate, when second substrate 2 is cover plate, anti-shrinkage groove 5 is in the frame-shaped vibrational power flow with opening on the internal surface 14 of cover plate, and this anti-shrinkage groove 5 is arranged near boss 4, and the anti-shrinkage groove 5 be positioned on outside surface 15 is arranged in the same fashion; Hatch frame 6 is arranged on second substrate 2, and hatch frame 6 is positioned at the opening of anti-shrinkage groove 5, as shown in Figure 3 and Figure 4.The internal surface 14 and outside surface 15 of second substrate 2 all arrange anti-shrinkage groove 5, and (setting position, direction, size, length etc. are all identical) is set in the same way, and this anti-shrinkage groove 5 is arranged near boss 4, farthest can reduce the deformation extent of second substrate 2 middle part, ensure that the chamber wall 3 of counting cell cavity has enough planeness to ensure to count effect.Hatch frame 6 is made to be positioned at the opening shrinking groove, can make to flow directly into anti-shrinkage groove 5 through hatch frame 6 sample entered in counting cell cavity, and under the guiding of anti-shrinkage groove 5, make sample liquid be guided to other position of counting cell more fast, uniformly.This kind of set-up mode, can make anti-shrinkage groove 5 while playing preventing deformation effect, also can play guide functions to sample, thus improves the result of use of tally further.
In another kind of embodiment, box body is be positioned at bottom for capped end box, and first substrate 1 is the cover plate for covering end box, and second substrate 2 is base plate, as shown in Figure 5 and Figure 6.Above-mentioned two kinds of structures, all can form counting cell cavity by box body with coordinating of first substrate 1, and it is two kinds of preferred structure formations.
When first substrate 1 is cover plate, when second substrate 2 is base plate, anti-shrinkage groove 5 is closed frame-shaped vibrational power flow on the internal surface 14 of base plate, and the anti-shrinkage groove 5 be positioned on outside surface 15 is arranged in the same fashion; Hatch frame 6 is arranged on the edge of cover plate side, and hatch frame 6 is oppositely arranged with the position of the anti-shrinkage groove 5 on base plate, as shown in Figure 7.This kind of set-up mode has the deformation extent reducing second substrate 2 middle part equally, ensures that the chamber wall 3 of counting cell cavity has enough planeness, and makes anti-shrinkage groove 5 while playing preventing deformation effect, also can play guide functions to sample.
Concrete, in the present embodiment, with as on boss 4 corresponding to hatch frame 6 position on the first substrate 1 of cover plate, have and also can be understood as the edge of second substrate 2 near wall 3() the arc notch 13 of direction depression, as shown in Fig. 5, Fig. 6 (in figure dotted portion) and Fig. 7, and the depression direction forming the arc groove of hatch frame 6 is on first substrate 1 contrary with the depression direction of arc notch 13, thus common formation is similar to the sample admission passage of circular hole.
In addition, the end face of wall 3 is symmetrically arranged with multiple can support baseboard substrate depart from work top, the projection of the strip be scratched for preventing baseplate substrate.The cell counting count board that the present embodiment provides, operationally, second substrate 2 can be made as counting cell upper cover plate, using first substrate 1 as counting cell lower cover slip, namely first substrate 1 is as microscope slide, as shown in Figure 1; Also can using second substrate 2 as counting cell lower cover slip, namely second substrate 2 is as microscope slide, first substrate 1 as counting cell upper cover plate, as shown in Figure 5.Wherein, preferred first substrate 1 is as microscope slide.When using first substrate 1 as lower cover slip, the projection be arranged on the end face of wall 3 can be played a supporting role to box body and the first substrate 1 be embedded in box body, make first substrate 1 depart from worktable, and then avoid first substrate 1 to cause wearing and tearing as worktable during lower cover slip to it.
Preferably, base plate is provided with focusing mark 16 or counting grid 17, as shown in Figure 10 and Figure 11.Wherein, focusing mark 16 is convenient to microscopical focusing, and counting grid 17 is convenient to the counting of sample.Concrete, this focusing mark 16 or counting grid 17 are arranged on counting cell bottom surface, this counting cell bottom surface is the face that first substrate 1 forms counting cell cavity, thus makes focusing mark 16 or counting grid 17 normally can play the object of focusing reference or counting reference.The cell counting count board that the present embodiment provides, when first substrate 1 is base plate, this focusing mark 16 or counting grid 17 arrange on first substrate 1, can not certainly do any mark.
In order to further mature technology scheme, the cell counting count board that the present embodiment provides, box body is be positioned at same plane multiple, and each box body all has a first substrate 1 to be mated forms counting cell cavity.The box body that the present embodiment provides and first substrate 1, singly can organize and be set to cell counting count board separately, also the many groups of box bodys cooperatively interacted and first substrate 1 jointly can be formed cell counting count board, to meet different counting needs in the mode of tiling in the plane.
This application provides a kind of mould, for the manufacture of the box body as hamper in cell counting count board described above, comprise upper bolster and hamper die shoe (not shown in FIG.), wherein:
Upper bolster is the stepped flange in bottom surface, the bottom surface of the convex part of flange is provided with the upper raised line protruding bottom surface;
Coordinate the hamper die shoe forming injection molding internal space with upper bolster, the end face of hamper die shoe is provided with the projection that can form hatch frame on box body; The edge of hamper die shoe is provided with the frame protruding die shoe end face, frame offers the opening for feed running through frame in the horizontal direction; The end face of hamper die shoe is provided with the lower raised line protruding end face.
As shown in Figure 12-Figure 14, present invention also provides the another kind of mould for the manufacture of the box body as end box, it is identical with structure of upper die base above-mentioned that this mould comprises upper bolster 7(upper bolster 7 herein, is same die holder) and end box die shoe 8, wherein:
Upper bolster 7 is the stepped flange in bottom surface, the bottom surface of the convex part of flange is provided with the upper raised line 9 protruding bottom surface;
The end box die shoe 8 forming injection molding internal space is coordinated with upper bolster 7, the end face of end box die shoe 8 is provided with the lower raised line 12 protruding end face, the edge of end box die shoe 8 arranges the frame 10 protruding end box die shoe 8 end face, frame 10 offers the opening for feed 11 running through frame 10 in the horizontal direction.
The box body that end box die shoe 8 and the injection moulding of hamper die shoe obtain is respectively end box and hamper, end box die shoe 8 is with the difference of hamper die shoe, hamper die shoe is provided with the projection that can form hatch frame 6 on the box body of injection moulding, thus the hamper obtained can be made to have hatch frame 6 to fill pond to application of sample in counting cell cavity.
In above-mentioned mould, because be provided with raised line 9 and lower raised line 12, thus can make to utilize the planar smoothness of the box body of above-mentioned mold injection higher, microscopical focusing accuracy can be improved preferably, the requirement to fine particle counting can be met, further increase the result of use of cell counting count board.
The application also provides a kind of method manufacturing cell counting count board, the box body of the cell counting count board that the method utilizes two kinds of Making mold described above described above, and it comprises the following steps:
S101, upper bolster 7 is fastened on die shoe (this die shoe refers to hamper die shoe or end box die shoe 8);
S103, to be coordinated to upper bolster 7 and die shoe by opening for feed 11 in the internal space that formed and inject injectable plastic material;
S104, make the injectable plastic material cooling forming that enters in internal space, obtain box body (this box body is hamper or end box);
S105, utilize other mold injection to form first substrate 1, and to assemble with box body, obtain complete cell counting count board.Wherein, first substrate 1 comprises and is provided with hatch frame 6 and is not provided with hatch frame 6 two kinds, and when box body is hamper, in this step, the first substrate 1 of indication is the substrate not being provided with hatch frame 6, when box body is end box, in this step, the first substrate 1 of indication is for being provided with the substrate of hatch frame 6.
In this specification sheets, each embodiment adopts the mode of going forward one by one to describe, and what each embodiment stressed is the difference with other embodiments, between each embodiment identical similar portion mutually see.
To the above-mentioned explanation of the disclosed embodiments, professional and technical personnel in the field are realized or uses the present invention.To be apparent for those skilled in the art to the multiple amendment of these embodiments, General Principle as defined herein can without departing from the spirit or scope of the present invention, realize in other embodiments.Therefore, the present invention can not be restricted to these embodiments shown in this article, but will meet the widest scope consistent with principle disclosed herein and features of novelty.
Claims (8)
1. a cell counting count board, is characterized in that, comprising: box body and first substrate (1), wherein:
Described box body comprises: second substrate (2) and the wall (3) be arranged at around described second substrate (2), and described second substrate (2) is provided with boss (4) along described wall (3) inner side;
Described second substrate (2) is provided with anti-shrinkage groove (5), and described anti-shrinkage groove (5) is arranged on the internal surface (14) that described second substrate (2) is provided with boss (4), and on the outside surface (15) relative with described internal surface (14), and the described anti-shrinkage groove (5) be positioned on described internal surface (14) is arranged along described boss (4);
Described first substrate (1) is embedded in described box body, and forms counting cell cavity with described second substrate (2), described boss (4);
Described counting cell cavity offers the hatch frame (6) filling pond for application of sample be communicated with described counting cell cavity.
2. cell counting count board according to claim 1, is characterized in that, described box body is hamper, and described first substrate is base plate, and described second substrate is cover plate.
3. cell counting count board according to claim 2, it is characterized in that, described anti-shrinkage groove (5) is in the frame-shaped vibrational power flow with opening on the internal surface (14) of described cover plate, and the described anti-shrinkage groove (5) be positioned on described outside surface (15) is arranged in the same fashion; Described second substrate (2) offers described hatch frame (6), and described hatch frame (6) is positioned at the opening of described anti-shrinkage groove (5).
4. cell counting count board according to claim 1, is characterized in that, described box body is end box, and described first substrate is cover plate, and described second substrate is base plate.
5. cell counting count board according to claim 4, it is characterized in that, described anti-shrinkage groove (5) is upper in closed frame-shaped vibrational power flow at the internal surface (14) of described base plate, and the described anti-shrinkage groove (5) be positioned on described outside surface (15) is arranged in the same fashion; Described hatch frame (6) is arranged on the edge of described cover plate side, and described hatch frame (6) is oppositely arranged with the position of the described anti-shrinkage groove (5) on described base plate.
6. a mould, is characterized in that, for the manufacture of the box body of cell counting count board described in the claims 1, comprises upper bolster and hamper die shoe, wherein:
Described upper bolster is the stepped flange in bottom surface, the bottom surface of the convex part of described flange is provided with the upper raised line protruding described bottom surface;
The hamper die shoe coordinated with described upper bolster, the end face of described hamper die shoe is provided with the projection that can form described hatch frame on described box body; The edge of described hamper die shoe is provided with the frame protruding described die shoe end face, described frame offers the opening for feed running through described frame in the horizontal direction; The end face of described die shoe is provided with the lower raised line protruding described end face.
7. a mould, is characterized in that, for the manufacture of the box body of cell counting count board described in the claims 1, comprises upper bolster and end box die shoe, wherein:
Described upper bolster is the stepped flange in bottom surface, the bottom surface of the convex part of described flange is provided with the upper raised line protruding described bottom surface;
The end box die shoe coordinated with described upper bolster, the end face of box die shoe of the described end is provided with the lower raised line protruding described end face, the edge of box die shoe of the described end arranges the frame protruding box die shoe end face of the described end, described frame offers the opening for feed running through described frame in the horizontal direction.
8. manufacture a method for cell counting count board, it is characterized in that, the method utilizes the box body of the cell counting count board described in Making mold the claims 1 described in the claims 6 or 7, and it comprises the following steps:
1) described upper bolster is fastened on die shoe;
2) in the internal space that described upper bolster and the cooperation of described die shoe are formed, injectable plastic material is injected by described opening for feed;
3) make the injectable plastic material cooling forming entered in described internal space, obtain described box body;
4) injection moulding forms first substrate, and assembles with described box body, obtains complete cell counting count board.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201410042530.2A CN103773671B (en) | 2014-01-28 | 2014-01-28 | Cell counting chamber, mould and method for manufacturing cell counting chamber |
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| CN201410042530.2A CN103773671B (en) | 2014-01-28 | 2014-01-28 | Cell counting chamber, mould and method for manufacturing cell counting chamber |
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| CN103773671A CN103773671A (en) | 2014-05-07 |
| CN103773671B true CN103773671B (en) | 2015-07-08 |
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Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107805596A (en) * | 2016-09-09 | 2018-03-16 | 东北农业大学 | A kind of direct counting box of bacterium for simple glass slide |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2377532Y (en) * | 1999-06-10 | 2000-05-10 | 中国人民解放军第二军医大学 | Microfluorecyte counting plate |
| CN201387415Y (en) * | 2009-03-19 | 2010-01-20 | 农业部沼气科学研究所 | Cell counting plate |
| CN202093554U (en) * | 2011-06-27 | 2011-12-28 | 深圳市博锐德生物科技有限公司 | Disposable cell counting plate |
| CN203807455U (en) * | 2014-01-28 | 2014-09-03 | 爱威科技股份有限公司 | Cell counting plate and die |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8372600B2 (en) * | 2004-03-05 | 2013-02-12 | The Research Foundation Of State University Of New York | Method and apparatus for measuring changes in cell volume |
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2377532Y (en) * | 1999-06-10 | 2000-05-10 | 中国人民解放军第二军医大学 | Microfluorecyte counting plate |
| CN201387415Y (en) * | 2009-03-19 | 2010-01-20 | 农业部沼气科学研究所 | Cell counting plate |
| CN202093554U (en) * | 2011-06-27 | 2011-12-28 | 深圳市博锐德生物科技有限公司 | Disposable cell counting plate |
| CN203807455U (en) * | 2014-01-28 | 2014-09-03 | 爱威科技股份有限公司 | Cell counting plate and die |
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| CN103773671A (en) | 2014-05-07 |
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Effective date of registration: 20180615 Address after: 410005 Ai Wei medical science and Technology Park, 26 Yuelu District Road, Yuelu District, Hunan Patentee after: Hunan aivin Medical Technology Co Ltd Address before: 410013 B6, 27 Yuan Lu Yuan Yuan, Wen Xuan Road, Changsha hi tech Development Zone, Hunan. Patentee before: AVE Science & Technology Co., Ltd. |