CN103766259B

CN103766259B - Fish Microexamination device

Info

Publication number: CN103766259B
Application number: CN201410037964.3A
Authority: CN
Inventors: 莫思特; 谢敏; 谭洁; 李碧雄
Original assignee: Sichuan University
Current assignee: Sichuan University
Priority date: 2014-01-26
Filing date: 2014-01-26
Publication date: 2015-11-18
Anticipated expiration: 2034-01-26
Also published as: CN103766259A

Abstract

The invention discloses a kind of fish Microexamination device.Device is made up of fish culture box, imbibition infusion set, microscopic image acquisition device, conveyer, collection box, server and controller.Server is controlled fish culture box, conveyer, infusion set by controller.After the fish of controller to fish culture box controls, fish in fish culture box is delivered to conveyer, input medicine by infusion set, then deliver to microscopic image acquisition device by conveyer and carry out micro image collection, the view data of microscopic image acquisition device collection flows to server; Fish through Germicidal efficacy is put back to collection box after gathering image by microscopic image acquisition device.Invention automatically can catch fishes snd, automatically to fishes snd injection, automatically carry out microexamination and record to fishes snd.Whole system is carried out under computer, greatly can improve the microexamination efficiency of fishes snd, for fish high flux, broad scale research provide research equipment.

Description

Fish Microexamination device

(1) technical field

The present invention relates to a kind of life science device, specifically fish Microexamination device.

(2) background technology

Fish behavior and state observation have significant role in life science.The Chinese invention patent of application number CN200510047579 discloses a kind of immunofluorescence microscopy observation technology of left-eyed flounder zygote cytoskeleton; Application number CN201110137175 disclose a kind of be applicable to Stereo microscope under observe fish embryo grow method and device; Application number CN201320214094 discloses a kind of microinjection device imported for foreign gene; Application number CN201310332662 discloses the microscopy observation method of a kind of seawater left-eyed flounder zygote microtubule skeleton and nuclear double immunofluorescense.Above invention discloses the Microexamination device of some fish life studies, but be all for a special application.In retrieved similar invention, automatically do not catch fishes snd and carry out the device of microexamination after drug injection is carried out to sample.

(3) summary of the invention

The object of this invention is to provide and a kind ofly automatically can catch fishes snd and carry out the device of microexamination after drug injection is carried out to sample.It is wide that this device is suitable for research range, can be fish high flux, broad scale research provides research equipment.

The object of the invention is to reach like this: device is made up of fish culture box, imbibition infusion set, microscopic image acquisition device, conveyer, collection box, server and controller.Server is controlled fish culture box, conveyer, imbibition infusion set by controller; Fish culture box is containing fish jar and effuser, and effuser is contained in the bottom surface any part of fish jar; Described conveyer, containing dress fish entrance, puts fish outlet, transfer control, dress fishnet lattice, dress fishnet lattice guide rail, fish position detecting device.

The dress fish entrance of the delivery port aligned transmissions device of effuser on fish culture box; Put fish outlet collection box.

Described imbibition infusion set, microscopic image acquisition device are arranged on the side of transmitting device, and imbibition infusion set comprises liquid sucking device and infusion set.

After the fish of controller to fish culture box controls, fish in fish culture box is delivered to conveyer, input medicine by infusion set, then deliver to microscopic image acquisition device by conveyer and carry out micro image collection, the view data of microscopic image acquisition device collection flows to server; Fish through Germicidal efficacy is put back to collection box after gathering image by microscopic image acquisition device.

Described fish culture box is made up of fish jar, effuser, fish detection light emitting diode, fish detection photodiode, delivery port, water plugging piece, water blockoff spring, water plugging piece support, water blockoff connecting rod, the stay cord that discharges water, the motor that discharges water.

Store water in fish jar and put fish; Effuser is contained in the bottom surface any part of fish jar, and adjutage is that transparent material makes; Fish detects light emitting diode, fish detects the both sides that photodiode is placed on adjutage, and the light transmission adjutage that fish detection light emitting diode sends is radiated at fish and detects on photodiode.Delivery port is the water export of fish jar.Water plugging piece is the circle of diameter slightly larger than effuser diameter, and the one side towards delivery port installs caulking gum.Water blockoff connecting rod and water plugging piece are fastenedly connected, water blockoff connecting rod is arranged on water plugging piece support, and water plugging piece support one end is fixedly mounted on fish jar, and the other end installs water blockoff connecting rod, water blockoff connecting rod can rotate around mounting points, and water plugging piece support installing is directly over the effuser of fish jar; Water blockoff spring one end is fixedly mounted on fish jar, one end connects water blockoff connecting rod, water blockoff spring the mounting points of fish jar at water plugging piece support between the mounting points and effuser of fish jar, water blockoff spring at the tie point of water blockoff connecting rod between the tie point and water plugging piece of water blockoff connecting rod and water plugging piece support; Discharge water one end of stay cord connects the rotating shaft of motor of discharging water, and the other end connects water blockoff connecting rod, is positioned at the top of the tie point of water plugging piece support and water blockoff connecting rod with the tie point of water blockoff connecting rod; The motor that discharges water is arranged on fish jar, and mounting points is positioned at directly over the mounting points of water plugging piece support on fish jar.

Described conveyer is made up of three-shaft displacement adjusting device of infusing, micro-three-shaft displacement adjusting device, transfer control, dress fishnet lattice, dress fishnet lattice guide rail, fish position detecting device.In the delivery port position of fish culture box, dress fishnet lattice guide rail is provided with dress fish entrance, and above collection box, dress fishnet lattice guide rail is provided with puts fish outlet.The both sides of infusion set and microscopic image acquisition device dress fishnet lattice guide rail is provided with flexible dress fishnet lattice guide rail.

Dress fish entrance, fish position detecting device, imbibition infusion set, microscopic image acquisition device are arranged in order on conveyer, transfusion three-shaft displacement adjusting device is corresponding with the infusion set of imbibition infusion set, and micro-three-shaft displacement adjusting device is corresponding with microscopic image acquisition device.

Dress fishnet lattice guide rail is two parallel ring-shaped guide rails, the relative side of closed slide convinces groove by patient analysis, opposed guide slots spacing equals the spacing filled outside the Guide rollers of fishnet lattice, multiple dress fishnet lattice are connected to each other by connection hole, form ring-type, be arranged on dress fishnet lattice guide rail by the Guide rollers of dress fishnet lattice;

The infusion set of imbibition infusion set and the both sides dress fishnet lattice guide rail of microscopic image acquisition device there is flexible dress fishnet lattice guide rail.

Described imbibition infusion set comprises clean injectors dish, syringe mounting strap, transfusion storage tank, liquid sucking device, infusion set, syringe, useless syringe recovering tray, transfusion device move and control motor, transfusion trapped orbit device.

Syringe is arranged on syringe mounting strap, and clean injectors is wrapped on clean injectors dish by syringe mounting strap, and syringe mounting strap controls syringe mobile route by transfusion trapped orbit device, and finally moves on useless syringe recovering tray.Useless syringe recovering tray installs transfusion device and moves control motor, transfusion device moves and controls driven by motor and to give up the rotation of syringe recovering tray, drive syringe mounting strap to move to useless syringe recovering tray by the rotation of useless syringe recovering tray, syringe mounting strap is wrapped on useless syringe recovering tray.

Described transfusion trapped orbit device is made up of clean injectors side transfusion positioning guide rail, useless syringe recovering tray transfusion positioning guide rail, liquid sucking device positioning guide rail, infusion set positioning guide rail.Four positioning guide rails are rotary cylinder, and cylinder embeds syringe mounting strap; Clean injectors side transfusion positioning guide rail, useless syringe recovering tray transfusion positioning guide rail are arranged on flexible fastening arm; Liquid sucking device positioning guide rail, infusion set positioning guide rail are arranged on liquid sucking device, infusion set respectively;

Described liquid sucking device is made up of liquid sucking device positioning guide rail and piston pushing mechanism, and liquid sucking device is arranged on the top of transfusion storage tank; Liquid sucking device positioning guide rail is at rotary positioning guide rail cylinder bayonet socket setting up and down, and the above chuck in one's ear on being is stack shell chuck below; Stack shell chuck and upper chuck are in one's ear circle, and every 90 degree of designs bayonet socket in circle, block the stack shell of syringe and the in one's ear upper of syringe respectively, in one's ear chuck be stuck in the upper top in one's ear of syringe.Piston pushing mechanism comprises piston chuck, piston catch bar, piston eccentric wheel and piston and promotes bearing of motor, and piston promotes bearing of motor and is connected with the turning cylinder that piston promotes electromotor; Be piston catch bar directly over piston is eccentric, piston catch bar is arranged on piston chuck center, and piston promotes bearing of motor in the non-middle part of piston eccentric wheel; When piston promotes the rotation of motor driven piston eccentric wheel, piston catch bar is driven to move up and down, thus drive piston chuck to move up and down, piston chuck blocks bright following of syringe piston, when piston chuck moves up, drive syringe piston handle to move up, syringe sucks the liquid in transfusion storage tank.

Described infusion set is made up of positioning guide rail support spring of infusing, transfusion positioning guide rail support arm, acupuncture treatment motor bearings, acupuncture treatment eccentric wheel, transfusion pushing disk, pushing disk support spring, stressed dish of infusing, transfusion motor bearings, transfusion eccentric wheel.

Infusion set positioning guide rail is identical with the structure of liquid sucking device positioning guide rail, but in one's ear chuck upper in one's ear following of syringe; Infusion set positioning guide rail is arranged in transfusion positioning guide rail resilient support arms.Acupuncture treatment motor bearings is connected with acupuncture treatment motor rotation axis, when acupuncture treatment electromotor rotates, drives acupuncture treatment eccentric wheel to rotate.Transfusion positioning guide rail support spring makes transfusion positioning guide rail support arm support infusion set positioning guide rail upwards.

Transfusion positioning guide rail support spring supports transfusion positioning guide rail and support arm infusion set positioning guide rail upwards, when eccentric wheel of having an acupuncture treatment rotates, transfusion positioning guide rail support arm is driven to move up and down, when positioning guide rail support arm of infusing moves down, the pinprick of syringe is entered to fill in the fish body of fishnet lattice.

Transfusion pushing disk is connected by a cylindrical connecting rods with the stressed dish of transfusion.Pushing disk support spring supports the stressed dish of transfusion upwards, thus makes transfusion pushing disk upward; Transfusion motor bearings is connected with transfusion motor rotary shaft, the rotation of transfusion electromotor drives transfusion eccentric wheel to rotate, and transfusion eccentric wheel rotates and causes transfusion pushing disk to move up and down, when pushing disk of infusing moves down, move down bright for promotion piston, thus realize infusion function.

The transfusion three-shaft displacement adjusting device of described conveyer is identical with the structure of micro-three-shaft displacement adjusting device, comprises movable controlling motor, left and right moving control motor, moving up and down and control motor and movablely control bearing, left and right moving control bearing, move up and down and control bearing and detection and localization photodiode, detection and localization light emitting diode, Fixing clamp-seat, universal wheel; Movable control motor, left and right moving control motor, move up and down and control motor and drive respectively and movablely control bearing, left and right moving control bearing, move up and down the rotation controlling bearing.

The movable motor that controls is fixedly mounted in support platform, is arranged on mobile foundation front, and movable control bearing is outer silk screw thread, with the internal thread threaded engagement in mobile foundation front; Left and right moving control motor is fixedly mounted in support platform, is arranged on the right of mobile foundation, and left and right moving control bearing is outer silk screw thread, with the internal thread threaded engagement on the mobile foundation right side; Move up and down and control motor and be fixedly mounted on centre position above mobile foundation, moving up and down and controlling bearing is outer silk screw thread, with the internal thread threaded engagement of dress fishnet lattice supporting base.

Universal wheel is contained on 4 angles below mobile foundation;

Dress fishnet lattice support platform is fixedly mounted on dress fishnet lattice supporting base, and identical with the move mode of dress fishnet lattice supporting base, dress fishnet lattice support platform four angles are provided with the fixing dress fishnet lattice of Fixing clamp-seat.

Detection and localization photodiode and the up and down vertical opposite installation of detection and localization light emitting diode.

The transfer control of described conveyer is made up of transfer control motor, transfer control gear.Transfer control gear is engaged with the dress fishnet lattice transmission gear of dress fishnet lattice; Transfer control gear connects transfer control motor, transfer control driven by motor transfer control pinion rotation, thus drives dress fish Grid Mobile;

The dress fishnet lattice of described conveyer are made up of net grid support, Guide rollers and dress fishnet pocket.The mutual closed loop of multiple net grid support connects, and Guide rollers is arranged on the Guide rollers installing rack of net grid support, is limited in by the mobile route of net grid support on the path of dress fishnet lattice guide rail.

Dress fish entrance and the opposed guide slots spacing of putting fish exit of the dress fishnet lattice guide rail of described conveyer are less than the spacing outside the Guide rollers of front and back, make dress fishnet pocket through becoming opening shape herein; Fill fish entrance and put fish exit dress fishnet lattice guide rail, make dress fishnet pocket fill fish porch opening upwards, make dress fishnet pocket put fish exit opening to downwards;

Described fish position detecting device is made up of end face video camera and side cameras, the relative program that video camera is run by server controls, the top plane view picture of end face video camera shooting fish, the side image of side cameras shooting fish, carries out judgement by Computer Image Processing algorithm to the end face of fish and lateral location and calculates.

Described Fixing clamp-seat in Fixing clamp-seat shell, install Fixing clamp-seat spring additional and gland is formed, and Fixing clamp-seat shell is cylindrical shape, and Fixing clamp-seat spring is equipped with in the inside, and it is hemispherical that gland exposes Fixing clamp-seat housing parts.

Described net grid support is used for fixing dress fishnet pocket, net grid support is metallic support, before and after metallic support, there are 4 symmetrical Guide rollers installing racks side, Guide rollers installing rack has 4 Guide rollers installing holes fixedly mount 4 Guide rollerses, dress fishnet pocket is fixed on net grid support by dress fishnet pocket installing hole.Before and after metallic support, the connection of side is elasticity connecting bridge, and Guide rollers spacing can be changed with the guide rail spacing change of dress fishnet lattice.The metallic support left and right sides has 4 symmetrical connection holes to connect adjacent mesh support.

The position one_to_one corresponding of 4 fixing holes, detection and localization hole and 4 Fixing clamp-seats, detection and localization photodiode and detection and localization light emitting diodes; Make the gland of 4 Fixing clamp-seats can pass 4 fixing holes, and now, the light of detection and localization light emitting diode is irradiated on detection and localization photodiode right through detection and localization hole simultaneously.

The netted string bag is made into netted by flexible filament, two-layer, three edge sealings, and openings at one side forms a mesh bag, is arranged on string bag fixed frame.String bag fixed frame is metal frame, and three limits are single-layer metal, corresponding three limits of installing the sealing of the netted string bag; While be double-level-metal, netted string bag opening is installed; One side of the corresponding string bag opening of string bag fixed frame is flexible, and under the effect not having power, opening seals by natural resiliency.When oriented interior External Force Acting produces inside deformation, form an opening; String bag fixed frame has 4 symmetrical string bag fixed installation holes, corresponding with dress fishnet pocket installing hole;

Described server is controlled fish culture box, conveyer, infusion set by controller;

The circuit structure of controller and the associated motor of control comprise transfusion control communication interface circuit, three-shaft displacement controls communication interface circuit, transfusion controls microprocessor, three-shaft displacement controls microprocessor; Transfusion controls communication interface circuit connection server and controls microprocessor with transfusion, and three-shaft displacement controls communication interface circuit connection server and three-shaft displacement controls microprocessor;

Transfusion controls microprocessor connection fish detection luminous tube circuit, detection and localization circuit of LED, fish detects photodiode circuit, detection and localization photodiode circuit, discharging water controls electric machine controller, transfer control electric machine controller, piston pushing motor controller, acupuncture treatment electric machine controller, electric machine controller of infusing;

The control electric machine controller that discharges water connects the electromotor that discharges water; Transfer control electric machine controller connects transfer control electromotor; Piston pushing motor controller connects piston and promotes electromotor; Acupuncture treatment electric machine controller connects acupuncture treatment electromotor; Transfusion electric machine controller connects transfusion electromotor

Electric machine controller before and after electric machine controller, micro-three axle power-on and power-off machine controllers, micro-three axle left and right electric machine controllers, micro-three axles before and after three-shaft displacement control microprocessor connection transfusion three axle power-on and power-off machine controller, infuse three axle left and right electric machine controllers, three axles of infusing;

Three axle power-on and power-off machine controllers of infusing connect transfusion three axle and control electromotor up and down; Infuse about three axle left and right electric machine controllers connect three axles and control electromotor; Electric machine controller before and after three axles of infusing connects before and after transfusion three axle and controls electromotor; Micro-three axles of micro-three axle power-on and power-off machine controllers connection control electromotor, micro-three axle left and right electric machine controllers up and down and control about connecting micro-three axles to control electromotor before and after micro-three axles of electric machine controller connection before and after electromotor, micro-three axles.

The beneficial effect of the invention is: 1, automatically catch fishes snd; 2, automatically to fishes snd injection; 3, automatically microexamination is carried out and record to fishes snd; 4, whole system is carried out under computer, greatly can improve the microexamination efficiency of fishes snd; 5, for fish high flux, broad scale research provide research equipment.

Four, accompanying drawing explanation

Fig. 1 is general structure schematic diagram of the present invention.

Fig. 2 is fish culture box structural representation.

Fig. 3 is conveyer structural representation.

Fig. 4 is transfusion three-shaft displacement adjusting device and micro-three-shaft displacement adjusting device structural representation.

Fig. 5 is the profile of Fixing clamp-seat.

Fig. 6 transfer control motor schematic diagram.

Fig. 7 is dress fishnet lattice structure schematic diagram.

Fig. 8 is net grid support structural representation.

Fig. 9 is Guide rollers schematic diagram.

Figure 10 fills fishnet pocket schematic diagram.

Figure 11 is the dress fishnet pocket schematic diagram of opening.

Figure 12 is dress fishnet lattice guide rail, flexibility fills fishnet lattice guide rail, dress fish entrance and put fish exports scheme of installation.

Figure 13 is fish position detecting device scheme of installation.

Figure 14 is imbibition infusion set structural representation.

Figure 15 is transfusion trapped orbit device schematic diagram.

Figure 16 is liquid sucking device positioning guide rail schematic diagram.

Figure 17 is stack shell chuck and the upper schematic diagram of chuck in one's ear.

Figure 18 is piston pushing mechanism end view.

Figure 19 is liquid sucking device scheme of installation.

Figure 20 is the imbibition schematic diagram of liquid sucking device.

Figure 21 is infusion set structural representation.

Figure 22 is controller controling circuit structure and control mode schematic diagram.

Figure 23 is that transfusion controls communication interface circuit and three-shaft displacement controls communication interface circuit figure.

Figure 24 is that transfusion controls microprocessor and three-shaft displacement microcontroller circuit figure.

Figure 25 is fish light emitting diode testing circuit and positioning luminous diode detecting circuit figure.

Figure 26 is that fish detects photodiode circuit and detection and localization photodiode circuit diagram

Figure 27 electric machine controller and motor circuit figure.

Figure 28 is server program flow chart.

Figure 29 is that transfusion controls microprocessor program flow chart.

In figure, 1 fish jar, 2 effusers, 3 fish detect light emitting diode, 4 fish detect photodiode, 5 delivery ports, 6 water plugging pieces, 7 water blockoff springs, 8 water plugging piece supports, 9 water blockoff connecting rods, 10 discharge water stay cord, 11 discharge water motor, 12 fish culture boxes, 13 imbibition infusion sets, 14 microscopic image acquisition devices, 15 collection boxes, 16 transfusion three-shaft displacement adjusting devices, 17 micro-three-shaft displacement adjusting devices, 18 transfer controls, 19 dress fishnet lattice, 20 dress fishnet lattice guide rails, 21 fish position detecting devices, 22 dress fish entrances, 23 put fish outlet, 24-1, 24-2, 24-3, 24-5, 24-6 flexibility dress fishnet lattice guide rail, 25 mobile foundations, 26 support platforms, 27 movable control motors, 28 movable control bearings, 29 left and right moving control bearings, 30 left and right moving control motors, 31 move up and down control motor, 32 move up and down control bearing, 33 dress fishnet lattice supporting bases, 34 dress fish lattice support, 35 detection and localization photodiodes, 36 detection and localization light emitting diodes, 37-1, 37-2, 37-3, 37-4 Fixing clamp-seat, 38-1, 38-2, 38-3, 38-4 universal wheel, 39 Fixing clamp-seat shells, 40 Fixing clamp-seat springs, 41 glands, 42 transfer control motors, 43 transfer control gears, 44 dress fishnet lattice transmission gears, 45 net grid supports, 46 dress fishnet pockets, 47-1, 47-2, 47-3, 47-4 Guide rollers, 48 detection and localization holes, 49-1, 49-2, 49-3, 49-4 connection hole, 50-1, 50-2, 50-3, 50-4 fixing hole, 51 elasticity connecting bridges, 52-1, 52-2, 52-3, 52-4 Guide rollers installing rack, 53-1, 53-2, 53-3, 53-4 fills fishnet pocket installing hole, 54-1, 54-2, 54-3, 54-4 Guide rollers installing hole, 55-1, 55-2, 55-3, 55-4 string bag fixed installation hole, 56 string bag fixed frames, the 57 netted string bags, 58 end face video cameras, 59 side cameras, 60 clean injectors dishes, 61 syringe mounting straps, 62 transfusion storage tanks, 63 liquid sucking devices, 64 infusion sets, 65 syringes, 66 useless syringe recovering tray, 67 transfusion devices move control motor, 68 transfusion trapped orbit devices, 68-1 clean injectors side positioning guide rail, 68-2 gives up syringe recovering tray side positioning guide rail, 68-3 liquid sucking device positioning guide rail, 68-4 infusion set positioning guide rail, 69 elasticity registration arm, 70 stack shell chucks, chuck in one's ear on 71, 72 piston chucks, 73 piston catch bars, 74 piston eccentric wheels, 75 pistons promote bearing of motor, 76 piston handles, on 77 in one's ear, 78 stack shells, 79 transfusion positioning guide rail support springs, 80 transfusion positioning guide rail support arms, 81 acupuncture treatment motor bearings, 82 acupuncture treatment eccentric wheels, 83 transfusion pushing disks, 84 pushing disk support springs, the stressed dish of 85 transfusion, 86 transfusion motor bearings, 87 transfusion eccentric wheels.

Five, embodiment

Drawings illustrate embodiments of the invention.

See accompanying drawing 1.

Device is made up of fish culture box 12, imbibition infusion set 13, microscopic image acquisition device 14, conveyer, collection box 15, server and controller.Server is controlled fish culture box, conveyer, imbibition infusion set by controller.Fish culture box is containing fish jar 1 and effuser 2, and effuser 2 is contained in the bottom surface any part of fish jar; Described conveyer, containing dress fish entrance 22, puts fish outlet 23, transfer control 18, dress fishnet lattice 19, dress fishnet lattice guide rail 20, fish position detecting device 21.

The dress fish entrance 22 of the delivery port 5 aligned transmissions device of effuser 2 on fish culture box; Put fish outlet 23 and aim at collection box.

Imbibition infusion set 13, microscopic image acquisition device 14 are arranged on the both sides of transmitting device.

After the fish of controller to fish culture box controls, fish in fish culture box is delivered to conveyer, input medicine by infusion set, then deliver to microscopic image acquisition device by conveyer and carry out micro image collection, the view data of microscopic image acquisition device collection flows to server; Fish through Germicidal efficacy is put back to collection box 15 after gathering image by microscopic image acquisition device.

Microscopic image acquisition device is connected with server, and for gathering the micro-image of fishes snd, the fishes snd on conveyer aimed at by the object lens of microscopic image acquisition device.The digital biomicroscope that Guangzhou Ming Mei photoelectricity technology corporation, Ltd. produces can be selected, model: ME11.

Collection box is a common fish box, and inside is equipped with to breed fish and is used water, the fish after observing for recovery experiment.

Server is common calculator, for gathering the micro-image data of micro image collection system, and being connected with controller by communication interface, being controlled by controller to fish culture box, infusion set, conveyer.

See accompanying drawing 2.Fish culture box is made up of fish jar 1, effuser 2, fish detection light emitting diode 3, fish detection photodiode 4, delivery port 5, water plugging piece 6, water blockoff spring 7, water plugging piece support 8, water blockoff connecting rod 9, the stay cord 10 that discharges water, the motor 11 that discharges water.

Store water in fish jar and put fish; Effuser is contained in the bottom surface any part of fish jar, and adjutage is that transparent material makes; Fish detects light emitting diode 3 and fish detects the both sides that photodiode 4 is placed on adjutage, and the light transmission adjutage that fish detection light emitting diode 3 sends is radiated at fish and detects on photodiode 4; Delivery port is the water export of fish jar; Water plugging piece 6 is the circle of diameter slightly larger than effuser diameter, and the one side towards delivery port installs caulking gum; Water blockoff connecting rod 9 and water plugging piece are fastenedly connected, water blockoff connecting rod 9 is arranged on water plugging piece support 8, and water plugging piece support 8 one end is fixedly mounted on fish jar 1, and the other end installs water blockoff connecting rod, water blockoff connecting rod 9 can rotate around mounting points, and water plugging piece support 8 is arranged on directly over the effuser 2 of fish jar; Water blockoff spring 7 one end is fixedly mounted on fish jar 1, one end connects water blockoff connecting rod 9, water blockoff spring the mounting points of fish jar at water plugging piece support between the mounting points and effuser of fish jar, water blockoff spring at the tie point of water blockoff connecting rod between the tie point and water plugging piece of water blockoff connecting rod and water plugging piece support; Discharge water one end of stay cord connects the rotating shaft of motor of discharging water, and the other end connects water blockoff connecting rod, is positioned at the top of the tie point of water plugging piece support and water blockoff connecting rod with the tie point of water blockoff connecting rod; The motor that discharges water is arranged on fish jar, and mounting points is positioned at directly over the mounting points of water plugging piece support on fish jar.When the motor that discharges water rotates forward, strain the stay cord that discharges water, make water plugging piece away from delivery port, release the water of fish jar.When the motor that discharges water reversely rotates, the stay cord that discharges water loosens, and under the pulling force of water blockoff spring, water plugging piece is blocked delivery port by water blockoff connecting rod, and the water of fish jar cannot be outflowed.

See accompanying drawing 3-13.

Visible in Fig. 3, conveyer is made up of three-shaft displacement adjusting device 16 of infusing, micro-three-shaft displacement adjusting device 17, transfer control 18, dress fishnet lattice 19, dress fishnet lattice guide rail 20, fish position detecting device 21.In the delivery port position of fish culture box, dress fishnet lattice guide rail is provided with dress fish entrance 22, and above collection box, dress fishnet lattice guide rail is provided with puts fish outlet 23; The both sides of infusion set and microscopic image acquisition device dress fishnet lattice guide rail is provided with flexible dress fishnet lattice guide rail 24-1,24-2,24-3,24-5,24-6.

The dress fish entrance of dress fishnet lattice guide rail, the fish making fish culture box export out loads dress fishnet lattice, and the dress fishnet lattice guide rail above collection box puts fish outlet, for putting back to collection box with from filling fishnet lattice after Germicidal efficacy is complete.

Dress fish entrance 22, fish position detecting device 21, imbibition infusion set 13, microscopic image acquisition device 14 are arranged in order on conveyer, transfusion three-shaft displacement adjusting device 16 is corresponding with the infusion set of imbibition infusion set, and micro-three-shaft displacement adjusting device 17 is corresponding with microscopic image acquisition device 14.

Dress fishnet lattice guide rail 20 is two parallel ring-shaped guide rails, the relative side of closed slide convinces groove by patient analysis, opposed guide slots spacing equals the spacing filled outside the Guide rollers of fishnet lattice, fill multiple dress fishnet lattice to be connected to each other by connection hole, form ring-type, be arranged on dress fishnet lattice guide rail by the Guide rollers of dress fishnet lattice.

See accompanying drawing 4.

The transfusion three-shaft displacement adjusting device 16 of conveyer is identical with the structure of micro-three-shaft displacement adjusting device 17, comprises movable controlling motor 27, left and right moving control motor 30, moving up and down and control motor 31 and movablely control bearing 28, left and right moving control bearing 29, move up and down and control bearing 32 and detection and localization photodiode 35, detection and localization light emitting diode 36, Fixing clamp-seat 37, universal wheel 38; Movable control motor, left and right moving control motor, move up and down and control motor and drive respectively and movablely control bearing, left and right moving control bearing, move up and down the rotation controlling bearing.

The movable motor 27 that controls is fixedly mounted in support platform 26, is arranged on mobile foundation 25 front, and movable control bearing is outer silk screw thread, with the internal thread threaded engagement in mobile foundation front; Left and right moving control motor 30 is fixedly mounted in support platform 26, is arranged on the right of mobile foundation 25, and left and right moving control bearing is outer silk screw thread, with the internal thread threaded engagement on the mobile foundation right side; Move up and down and control motor 32 and be fixedly mounted on centre position above mobile foundation 25, moving up and down and controlling bearing is outer silk screw thread, with the internal thread threaded engagement of dress fishnet lattice supporting base.

Universal wheel 38 is contained on 4 angles below mobile foundation.

Dress fishnet lattice support platform 34 is fixedly mounted on dress fishnet lattice supporting base 33, identical with the move mode of dress fishnet lattice supporting base, and dress fishnet lattice support platform four angles are provided with Fixing clamp-seat 37-1,37-2,37-3,37-4 fixing dress fishnet lattice 19.

Detection and localization photodiode 35 and detection and localization light emitting diode 36 is up and down vertical opposite is arranged on net grid support.

Fig. 5.Fixing clamp-seat 37 in Fixing clamp-seat shell 39, installs Fixing clamp-seat spring 40 additional and gland 41 is formed, and Fixing clamp-seat shell 39 is cylindrical shape, and Fixing clamp-seat spring is equipped with in the inside, and it is hemispherical that gland exposes Fixing clamp-seat housing parts.

Not having the position of fixing hole, gland is pressed in Fixing clamp-seat shell.When the fixing hole of dress fishnet lattice is through gland top, dress fishnet lattice are fixed in dress fishnet lattice support platform by gland under the effect of Fixing clamp-seat spring.

Fig. 6.The transfer control 18 of described conveyer is made up of transfer control motor 42, transfer control gear 43.Transfer control gear 43 is engaged with the dress fishnet lattice transmission gear 44 of dress fishnet lattice 19.Transfer control gear 43 connects transfer control motor 42, at transfer control driven by motor transfer control pinion rotation, thus drives dress fish Grid Mobile.

See accompanying drawing 7.The dress fishnet lattice 19 of conveyer are made up of net grid support 45, Guide rollers 47-1,47-2,47-3,47-4 and dress fishnet pocket 46, the mutual closed loop of multiple net grid support connects, Guide rollers is arranged on Guide rollers installing rack 54-1,54-2,54-3,54-4 of net grid support 45, is limited in by the mobile route of net grid support on the path of dress fishnet lattice guide rail.

See Fig. 8.The dress fish entrance 22 of the dress fishnet lattice guide rail 20 of described conveyer and put opposed guide slots spacing that fish exports 23 places and be less than spacing outside the Guide rollers of front and back, makes to fill fishnet pocket through becoming opening shape herein; Fill fish entrance and put fish exit dress fishnet lattice guide rail, make dress fishnet pocket fill fish porch opening upwards, make dress fishnet pocket put fish exit opening to downwards.

Guide rollers is arranged on Guide rollers installing rack by Guide rollers installing hole, is limited in by the mobile route of net grid support on the path of dress fishnet lattice guide rail.Guide rollers is thick middle, the pulley that both sides are thin, and there is installing hole centre, and its side is as Fig. 9.

Figure 10, Figure 11.4 dress fishnet pocket installing holes are at 4 angles of string bag fixed frame, and dress fishnet pocket installing hole 53-1,53-2,53-3,53-4 and the string bag fixedly mount hole 55-1,55-2,55-3,55-4 one_to_one corresponding, for dress fishnet pocket 46 is fixedly mounted on net grid support.Dress fishnet pocket is made into netted by flexible filament, and two-layer, three edge sealings, openings at one side forms a mesh bag, is arranged on string bag fixed frame 56.String bag fixed frame is metal frame, and three limits are single-layer metal, corresponding three limits of installing the sealing of the netted string bag; While be double-level-metal, netted string bag opening is installed.One side of the corresponding string bag opening of string bag fixed frame is flexible, and under the effect not having power, opening seals by natural resiliency.When having External Force Acting to produce inside deformation, form an opening.When having the inside deformation of External Force Acting, the dress fishnet pocket of opening is as Figure 11.

Figure 12 is dress fishnet lattice guide rail, flexibility fills fishnet lattice guide rail, dress fish entrance and put fish exports scheme of installation.Dress fishnet lattice guide rail is two parallel ring-shaped guide rails, and the relative side of closed slide convinces groove by patient analysis, and opposed guide slots spacing equals the spacing outside the Guide rollers of front and back, makes guide groove and Guide rollers close contact, thus limits the moving line of dress fishnet lattice.Fill multiple dress fishnet lattice to be connected to each other by connection hole, form ring-type, be arranged on dress fishnet lattice guide rail by Guide rollers.The both sides of infusion set and microscopic image acquisition device dress fishnet lattice guide rail there is flexible dress fishnet lattice Guide Rail Design, for lowering the relative position of self-contained fishnet lattice and microscopic image acquisition device or infusion set in the effect of micro-three-shaft displacement adjusting device or transfusion three-shaft displacement adjusting device.

The dress fish entrance 22 of the dress fishnet lattice guide rail 20 of conveyer and put opposed guide slots spacing that fish exports 23 places and be less than spacing outside the Guide rollers of front and back, makes to fill fishnet pocket through becoming opening shape herein; Fill fish entrance and put fish exit dress fishnet lattice guide rail, make dress fishnet pocket fill fish porch opening upwards, make dress fishnet pocket put fish exit opening to downwards.

Figure 13 fish position detecting device scheme of installation.Fish position detecting device 21 is made up of end face video camera 58 and side cameras 59, the relative program that video camera is run by server controls, the top plane view picture of end face video camera shooting fish, the side image of side cameras shooting fish, carries out judgement by Computer Image Processing algorithm to the end face of fish and lateral location and calculates.

See accompanying drawing 14-21.

Imbibition infusion set comprises clean injectors dish 60, syringe mounting strap 61, transfusion storage tank 62, liquid sucking device 63, infusion set 64, syringe 65, useless syringe recovering tray 66, transfusion device move and control motor 67, transfusion trapped orbit device 68.Syringe 65 is arranged on syringe mounting strap 61, clean injectors 65 is wrapped on clean injectors dish 60 by syringe mounting strap 61, syringe mounting strap 61 controls syringe mobile route by transfusion trapped orbit device 68, and finally moves on useless syringe recovering tray 66; Useless syringe recovering tray is installed transfusion device and move control motor 67, transfusion device moves and controls the rotation that motor 67 drives useless syringe recovering tray 66, drive syringe mounting strap 61 to move to useless syringe recovering tray by the rotation of useless syringe recovering tray, syringe mounting strap 61 is wrapped on useless syringe recovering tray 66.

Described transfusion trapped orbit device is made up of clean injectors side transfusion positioning guide rail, useless syringe recovering tray transfusion positioning guide rail, liquid sucking device positioning guide rail, infusion set positioning guide rail.Four positioning guide rails are rotary cylinder, and cylinder embeds syringe mounting strap.Clean injectors side transfusion positioning guide rail, useless syringe recovering tray transfusion positioning guide rail are arranged on flexible fastening arm 69-1,69-2; Liquid sucking device positioning guide rail, infusion set positioning guide rail are arranged on liquid sucking device 63, infusion set 64 respectively.

Described liquid sucking device is made up of liquid sucking device positioning guide rail and piston pushing mechanism.Liquid sucking device is arranged on the top of transfusion storage tank.Liquid sucking device positioning guide rail is at rotary positioning guide rail cylinder bayonet socket setting up and down, and the above chuck in one's ear on being is stack shell chuck below; Stack shell chuck and upper chuck are in one's ear circle, and every 90 degree of designs bayonet socket in circle, block the stack shell 78 of syringe 65 and syringe respectively upper in one's ear 77, in one's ear chuck 71 be stuck in syringe upper in one's ear 77 top; Piston pushing mechanism comprises piston chuck 72, piston catch bar 73, piston eccentric wheel 74 and piston and promotes bearing of motor 75, and piston promotes bearing of motor 75 and is connected with the turning cylinder that piston promotes electromotor; Be piston catch bar directly over piston eccentric wheel 74, piston catch bar is arranged on piston chuck center, and piston promotes bearing of motor 75 in the non-middle part of piston eccentric wheel 74; When piston promotes the rotation of motor driven piston eccentric wheel, piston catch bar 73 is driven to move up and down, thus drive piston chuck 72 to move up and down, piston chuck 72 seized piston bright 76 following, when piston chuck 72 moves up, drive syringe piston handle 76 to move up, syringe sucks the liquid in transfusion storage tank.

Described infusion set is made up of positioning guide rail support spring 79 of infusing, transfusion positioning guide rail support arm 80, acupuncture treatment motor bearings 81, acupuncture treatment eccentric wheel 82, transfusion pushing disk 83, pushing disk support spring 84, stressed dish 85 of infusing, transfusion motor bearings 86, transfusion eccentric wheel 87.

Infusion set positioning guide rail (68-4) is identical with the structure of liquid sucking device positioning guide rail (68-3), but in one's ear chuck 71 be stuck in syringe upper in one's ear 77 following; Infusion set positioning guide rail is arranged in transfusion positioning guide rail resilient support arms 80; Acupuncture treatment motor bearings 81 is connected with acupuncture treatment motor rotation axis, when acupuncture treatment electromotor rotates, drives acupuncture treatment eccentric wheel 82 to rotate; Transfusion positioning guide rail support spring 79 makes transfusion positioning guide rail support arm 80 support infusion set positioning guide rail upwards,

Transfusion positioning guide rail support spring 79 supports transfusion positioning guide rail support arm 80 and infusion set positioning guide rail upwards, when eccentric wheel 82 of having an acupuncture treatment rotates, transfusion positioning guide rail support arm 80 is driven to move up and down, when positioning guide rail support arm 80 of infusing moves down, the pinprick of syringe is entered to fill in the fish body of fishnet lattice.

Transfusion pushing disk 83 is connected by pushing disk one cylindrical connecting rods with the stressed dish 85 of transfusion; Pushing disk support spring 84 supports the stressed dish of transfusion upwards, thus makes transfusion pushing disk upward; Transfusion motor bearings 86 is connected with transfusion motor rotary shaft, the rotation of transfusion electromotor drives transfusion eccentric wheel 87 to rotate, and transfusion eccentric wheel rotates and causes transfusion pushing disk 83 to move up and down, when pushing disk 83 of infusing moves down, move down bright for promotion piston 76, thus realize infusion function.

Figure 23 is that transfusion controls communication interface circuit and three-shaft displacement controls communication interface circuit figure.Adopt RS232 interface circuit.In RS232 interface circuit, U8 is the MAX232 of U.S.'s Maxim.11,12 pins of U8 are connected with the transmitting-receiving pin of terminal server serial ports; 13,14 pins of U8 are connected with the corresponding pin of microprocessor with terminal transmission.

See Figure 24.It is identical with three-shaft displacement microcontroller circuit that transfusion controls microprocessor.Sensing control single chip circuit adopts the single-chip microcomputer MSP430F135 of TEXASINSTRUMENTS company, and U3 is memory integrated circuit, adopts the M24C64 that STMicroelectronics company produces.For storing information.

Figure 25.Fish light emitting diode testing circuit is identical with positioning luminous diode detecting circuit.

Figure 26.It is identical with detection and localization photodiode circuit that fish detects photodiode circuit.Burr-Brown company of U1 U.S. integrated circuit: model: OPT101

Figure 27 electric machine controller and motor circuit figure.In figure, M1: stepper motor driver: model: DM320C, manufacturer: Leisai Electromechanical Tech. Dev. Co., Ltd., Shenzhen City.M2: stepper motor: model: 35HS01, manufacturer: Leisai Electromechanical Tech. Dev. Co., Ltd., Shenzhen City.All electric machine controllers adopt identical circuit for controlling motor, and identical electromotor selected by all electromotor.In figure, the P1-P8 of each stepping motor control circuit is connected with the universal input/output interface of difference single-chip microcomputer.

See Figure 22.Server is controlled fish culture box, conveyer, infusion set by controller,

Its control procedure is: server and transfusion control communication interface circuit, three-shaft displacement controls communication interface circuit two-way communication, transfusion control and three axle control circuits are controlled, transfusion control communication interface circuit controls microprocessor with transfusion and realizes two-way communication, transfusion controls Microprocessor S3C44B0X fish detection luminous tube circuit, whether detection and localization circuit of LED is luminous, receives fish and detects photodiode circuit, detection and localization photodiode circuit signal, simultaneously, transfusion controls Microprocessor S3C44B0X and discharges water and control electric machine controller and then control and to discharge water electromotor, controls transfer control electric machine controller and then control transfer control electromotor, control piston pushing motor controller and then control piston to promote electromotor, control acupuncture treatment electric machine controller and then control acupuncture treatment electromotor, control transfusion electric machine controller and then control transfusion electromotor, three-shaft displacement controls Microprocessor S3C44B0X and to infuse three axle power-on and power-off machine controllers and then control transfusion three axle and control electromotor up and down, control transfusion three axle left and right electric machine controller and then control electromotor about controlling three axles, control to control electromotor before and after electric machine controller and then control transfusion three axle before and after transfusion three axle, control micro-three axle power-on and power-off machine controllers and then control micro-three axles to control electromotor up and down, control micro-three axle left and right electric machine controllers and then control electromotor about controlling micro-three axles, to control before and after micro-three axles electric machine controller and then control to control electromotor before and after micro-three axles.

Three-shaft displacement controls microprocessor program flow process:

The first step: by communication interface reception server control data;

Second step: according to movable control motor, the left and right moving control motor of server controls Data Control transfusion three-shaft displacement adjusting device, move up and down the rotation direction and rotational angle that control motor;

3rd step: according to the movable control motor of server controls Data Control micro-three-shaft displacement adjusting device, left and right moving control motor, move up and down the rotation direction and rotational angle that control motor;

4th step: return the first step.

See accompanying drawing 28.The program circuit of server is: establish M dress fishnet lattice of being separated by between dress fish entrance 22 and fish position detecting device 21; To be separated by between fish position detecting device 21 and infusion set 64 K dress fishnet lattice; To be separated by between infusion set 64 and microscopic image acquisition device 14 L dress fishnet lattice.

Start, the first step, sends to controller order of starting working by communication interface; Second step, the injection position of setting fish; The observation place of setting fish and range of observation; X=0; 3rd step, the n value of Read Controller; Does 4th step, judge n=x+1? no, return the 3rd step, be, the 5th step x=n; 6th step, n>M? no, return the 3rd step, be, the 7th step, read the view data of end face video camera and side cameras; 8th step, calculates the image edge of end face video camera and side cameras; 9th step, calculates the length of fish and wide according to the edge of end face camera review fish; The horizontal direction position of fish is determined according to the edge of end face camera review fish; The height of fish is calculated according to the edge of side cameras image of fish; The vertical direction position of fish is determined according to the edge of side cameras image of fish; Tenth step, do you judge n>M+K+1? no, return the 3rd step, be, 11 step, according to the injection position of the fish of the length of record K fish forward, the horizontal direction position of fish, the vertical direction position of fish and setting calculate transfusion three-shaft displacement adjusting device movable control motor, left and right moving control motor, move up and down the rotation direction and rotational angle that control motor, and calculated value is issued three-shaft displacement control microprocessor; 12 step, do you judge n>M+K+L+2? no, return the 3rd step, be, 13 step, according to the observation place of the fish of the length of record K+L+1 fish forward, the horizontal direction position of fish, the vertical direction position of fish and setting calculate micro-three-shaft displacement adjusting device movable control motor, left and right moving control motor, move up and down the rotation direction and rotational angle that control motor, and calculated value is issued three-shaft displacement control microprocessor; 14 step, is gathered by microscopic image acquisition device and stores micro-image; Does 15 step, judge whether to quit work? no, return the 3rd step, be, the 16 step, sends to controller the order that quits work, and terminates.

See Figure 29.Transfusion controls microprocessor-based control flow process:

Start, the first step, by the order of communication interface reception server, second step, judges whether to start infusion program, no, returns the first step, is, the 3rd step n=0; Fish detects lumination of light emitting diode; Detection and localization lumination of light emitting diode; 4th step, controls the motor that discharges water and discharges water, the 5th step, reads fish and detects photodiode sensitization data; Does 6th step, judge that fish detects photosensitive reading and is less than and has fish to judge thresholding? no, return the 5th step, be, the 7th step, control the motor pass water that discharges water; Control transfer control motor to rotate to direction of transfer; 8th step, reads detection and localization photodiode sensitization data, does the 9th step, judge that the photosensitive reading of detection and localization is greater than location determination thresholding? no, return the 8th step, be, the tenth step, n=n+1; Control transfer control motor stalls; Server is given by n by communication interface; 11 step, control piston pushing motor rotates, and liquid sucking device draws medicine; Does 12 step, judge n>(M+K+1)? no, enter the 15 step, be, the 13 step, control acupuncture treatment electromotor and rotate, pinprick enters in fish body by syringe; 14 step, control transfusion electromotor and rotate, syringe is by infusion of medicine fish body.15 step: control transfusion device and move control motor, makes syringe to move right the spacing of adjacent injectors; 16 step, by the order of communication interface reception server; 17 step, judges whether to quit work; Terminate, no, return the 4th step.

Claims

1. a fish Microexamination device, is characterized in that: device is made up of fish culture box (12), imbibition infusion set (13), microscopic image acquisition device (14), conveyer, collection box (15), server and controller; Server is controlled fish culture box, conveyer, imbibition infusion set by controller; Fish culture box is containing fish jar (1) and effuser (2), and effuser (2) is contained in the bottom surface any part of fish jar; Described conveyer is containing filling fish entrance (22), putting fish outlet (23), transfer control (18), dress fishnet lattice (19), dress fishnet lattice guide rail (20), fish position detecting device (21);

On fish culture box, the delivery port (5) of effuser (2) aims at the dress fish entrance (22) of conveyer; Put fish outlet (23) and aim at collection box (15);

Described imbibition infusion set (13), microscopic image acquisition device (14) are arranged on the side of conveyer, and imbibition infusion set (13) comprises liquid sucking device (63) and infusion set (64);

After the fish of controller to fish culture box controls, fish in fish culture box is delivered to conveyer, input medicine by infusion set, then deliver to microscopic image acquisition device by conveyer and carry out micro image collection, the view data of microscopic image acquisition device collection flows to server; Fish through Germicidal efficacy is put back to collection box (15) after gathering image by microscopic image acquisition device.

2. fish Microexamination device as claimed in claim 1, is characterized in that: described fish culture box is made up of fish jar (1), effuser (2), fish detection light emitting diode (3), fish detection photodiode (4), delivery port (5), water plugging piece (6), water blockoff spring (7), water plugging piece support (8), water blockoff connecting rod (9), the stay cord that discharges water (10), the motor that discharges water (11);

Store water in fish jar and put fish; Effuser is contained in the bottom surface any part of fish jar, and effuser is that transparent material makes; Fish detects light emitting diode (3) and fish detects the both sides that photodiode (4) is placed on effuser, and the light transmission effuser that fish detection light emitting diode (3) sends is radiated at fish and detects on photodiode (4); Delivery port is the water export of fish jar; Water plugging piece (6) is for diameter is slightly larger than the circle of effuser diameter, and the one side towards delivery port installs caulking gum; Water blockoff connecting rod (9) and water plugging piece are fastenedly connected, water blockoff connecting rod (9) is arranged on water plugging piece support (8), water plugging piece support (8) one end is fixedly mounted on fish jar (1), the other end installs water blockoff connecting rod, water blockoff connecting rod (9) can rotate around mounting points, and water plugging piece support (8) is arranged on directly over the effuser (2) of fish jar; Water blockoff spring (7) one end is fixedly mounted on fish jar (1), one end connects water blockoff connecting rod (9), water blockoff spring the mounting points of fish jar at water plugging piece support between the mounting points and effuser of fish jar, water blockoff spring at the tie point of water blockoff connecting rod between the tie point and water plugging piece of water blockoff connecting rod and water plugging piece support; Discharge water one end of stay cord connects the rotating shaft of motor of discharging water, and the other end connects water blockoff connecting rod, is positioned at the top of the tie point of water plugging piece support and water blockoff connecting rod with the tie point of water blockoff connecting rod; The motor that discharges water is arranged on fish jar, and mounting points is positioned at directly over the mounting points of water plugging piece support on fish jar.

3. fish Microexamination device as claimed in claim 1, is characterized in that: described conveyer is made up of three-shaft displacement adjusting device (16) of infusing, micro-three-shaft displacement adjusting device (17), transfer control (18), dress fishnet lattice (19), dress fishnet lattice guide rail (20), fish position detecting device (21); In the delivery port position of fish culture box, dress fishnet lattice guide rail is provided with dress fish entrance (22), and above collection box, dress fishnet lattice guide rail is provided with puts fish outlet (23); The both sides of infusion set and microscopic image acquisition device dress fishnet lattice guide rail is provided with flexible dress fishnet lattice guide rail (24-1,24-2,24-3,24-5,24-6);

Dress fish entrance (22), fish position detecting device (21), imbibition infusion set (13), microscopic image acquisition device (14) are arranged in order on conveyer, transfusion three-shaft displacement adjusting device (16) is corresponding with the liquid sucking device of imbibition infusion set, and micro-three-shaft displacement adjusting device (17) is corresponding with microscopic image acquisition device (14);

Dress fishnet lattice guide rail (20) is two parallel ring-shaped guide rails, the relative side of closed slide convinces groove by patient analysis, opposed guide slots spacing equals the spacing filled outside the Guide rollers of fishnet lattice, multiple dress fishnet lattice are connected to each other by connection hole, form ring-type, be arranged on dress fishnet lattice guide rail by the Guide rollers of dress fishnet lattice.

4. fish Microexamination device as claimed in claim 1, is characterized in that: described imbibition infusion set comprises clean injectors dish (60), syringe mounting strap (61), transfusion storage tank (62), liquid sucking device (63), infusion set (64), syringe (65), useless syringe recovering tray (66), transfusion device move and control motor (67), transfusion trapped orbit device (68);

Syringe (65) is arranged on syringe mounting strap (61), syringe (65) is wrapped on clean injectors dish (60) by syringe mounting strap (61), syringe mounting strap (61) controls syringe mobile route by transfusion trapped orbit device (68), and finally moves on useless syringe recovering tray (66); Useless syringe recovering tray installs transfusion device and moves control motor (67), transfusion device moves and controls the rotation that motor (67) drives useless syringe recovering tray (66), drive syringe mounting strap (61) to move to useless syringe recovering tray by the rotation of useless syringe recovering tray, syringe mounting strap (61) is wrapped on useless syringe recovering tray (66);

Described transfusion trapped orbit device (68) is made up of clean injectors side transfusion positioning guide rail (68-1), useless syringe recovering tray transfusion positioning guide rail (68-2), liquid sucking device positioning guide rail (68-3), infusion set positioning guide rail (68-4), four positioning guide rails are rotary cylinder, and cylinder embeds syringe mounting strap (61); Clean injectors side transfusion positioning guide rail, useless syringe recovering tray transfusion positioning guide rail are arranged on flexible fastening arm (69-1,69-2); Liquid sucking device positioning guide rail, infusion set positioning guide rail are arranged on liquid sucking device 63, infusion set 64 respectively;

Described liquid sucking device (63) is made up of liquid sucking device positioning guide rail (68-3) and piston pushing mechanism, and liquid sucking device is arranged on the top of transfusion storage tank (62); Liquid sucking device positioning guide rail (68-3) is at rotary positioning guide rail cylinder bayonet socket setting up and down, and the above chuck (71) in one's ear on being is stack shell chuck (70) below; Stack shell chuck and upper chuck are in one's ear circle, and every 90 degree of designs bayonet socket in circle, block the stack shell (78) of syringe (65) and upper (77) in one's ear of syringe respectively, in one's ear chuck (71) be stuck in the top of upper (77) in one's ear of syringe; Piston pushing mechanism comprises piston chuck (72), piston catch bar (73), piston eccentric wheel (74) and piston and promotes bearing of motor (75), and piston promotes bearing of motor (75) and is connected with the turning cylinder that piston promotes electromotor; Be piston catch bar directly over piston eccentric wheel (74), piston catch bar is arranged on piston chuck center, and piston promotes bearing of motor (75) in piston eccentric wheel (74) non-middle part; When piston promotes the rotation of motor driven piston eccentric wheel, piston catch bar (73) is driven to move up and down, thus drive piston chuck (72) to move up and down, piston chuck (72) blocks the following of syringe piston handle (76), when piston chuck (72) moves up, drive syringe piston handle (76) to move up, syringe sucks the liquid in transfusion storage tank;

Described infusion set is made up of positioning guide rail support spring (79) of infusing, transfusion positioning guide rail support arm (80), acupuncture treatment motor bearings (81), acupuncture treatment eccentric wheel (82), transfusion pushing disk (83), pushing disk support spring (84), stressed dish (85) of infusing, transfusion motor bearings (86), transfusion eccentric wheel (87);

Infusion set positioning guide rail (68-4) is identical with the structure of liquid sucking device positioning guide rail (68-3), but in one's ear chuck (71) be stuck in the following of upper (77) in one's ear of syringe; Infusion set positioning guide rail is arranged in transfusion positioning guide rail support arm (80); Acupuncture treatment motor bearings (81) is connected with acupuncture treatment motor rotation axis, when acupuncture treatment electromotor rotates, drives acupuncture treatment eccentric wheel (82) to rotate; Transfusion positioning guide rail support spring (79) makes transfusion positioning guide rail support arm (80) support infusion set positioning guide rail upwards,

Transfusion positioning guide rail support spring (79) support transfusion positioning guide rail support arm (80) and infusion set positioning guide rail upwards, when eccentric wheel (82) of having an acupuncture treatment rotates, transfusion positioning guide rail support arm (80) is driven to move up and down, when positioning guide rail support arm (80) of infusing moves down, the pinprick of syringe is entered to fill in the fish body of fishnet lattice;

Transfusion pushing disk (83) is connected by a cylindrical connecting rods with the stressed dish of transfusion (85); Pushing disk support spring (84) supports the stressed dish of transfusion upwards, thus makes transfusion pushing disk upward; Transfusion motor bearings (86) is connected with transfusion motor rotary shaft, the rotation of transfusion electromotor drives transfusion eccentric wheel (87) to rotate, transfusion eccentric wheel rotates and causes transfusion pushing disk (83) to move up and down, when pushing disk (83) of infusing moves down, piston handle (76) will be promoted move down, thus realize infusion function.

5. fish Microexamination device as claimed in claim 3, it is characterized in that: the transfusion three-shaft displacement adjusting device (16) of described conveyer is identical with the structure of micro-three-shaft displacement adjusting device (17), comprise movable control motor (27), left and right moving control motor (30), move up and down and control motor (31) and movable control bearing (28), left and right moving control bearing (29), move up and down and control bearing (32) and detection and localization photodiode (35), detection and localization light emitting diode (36), Fixing clamp-seat (37), universal wheel (38), movable control motor, left and right moving control motor, move up and down and control motor and drive respectively and movablely control bearing, left and right moving control bearing, move up and down the rotation controlling bearing,

The movable motor (27) that controls is fixedly mounted in support platform (26), is arranged on mobile foundation (25) front, and movable control bearing is outer silk screw thread, with the internal thread threaded engagement in mobile foundation front; Left and right moving control motor (30) is fixedly mounted in support platform (26), and be arranged on mobile foundation (25) the right, left and right moving control bearing is outer silk screw thread, with the internal thread threaded engagement on the mobile foundation right side; Move up and down and control the centre position that motor (31) is fixedly mounted on mobile foundation (25) top, moving up and down and controlling bearing is outer silk screw thread, with the internal thread threaded engagement of dress fishnet lattice supporting base;

Universal wheel (38) is arranged on 4 angles below mobile foundation;

Dress fishnet lattice support platform (34) is fixedly mounted on dress fishnet lattice supporting base (33), identical with the move mode of dress fishnet lattice supporting base, dress fishnet lattice support platform four angles are provided with Fixing clamp-seat (37-1,37-2,37-3,37-4) fixing dress fishnet lattice (19);

Detection and localization photodiode (35) and detection and localization light emitting diode (36) up and down vertical opposite installation;

The transfer control (18) of described conveyer is made up of transfer control motor (42), transfer control gear (43); Transfer control gear (43) is engaged with dress fishnet lattice transmission gear (44) of dress fishnet lattice (19); Transfer control gear (43) connects transfer control motor (42), transfer control driven by motor transfer control pinion rotation, thus drives dress fish Grid Mobile;

The dress fishnet lattice (19) of described conveyer are made up of net grid support (45), Guide rollers (47-1,47-2,47-3,47-4) and dress fishnet pocket (46), the mutual closed loop of multiple net grid support connects, Guide rollers is arranged on the Guide rollers installing rack (52-1,52-2,52-3,52-4) of net grid support 45, is limited in by the mobile route of net grid support on the path of dress fishnet lattice guide rail;

The dress fish entrance (22) of dress fishnet lattice guide rail (20) of described conveyer and the opposed guide slots spacing of putting fish outlet (23) place are less than the spacing outside the Guide rollers of front and back, make to fill fishnet pocket through becoming opening shape herein; Fill fish entrance and put fish exit dress fishnet lattice guide rail, make dress fishnet pocket fill fish porch opening upwards, Open Side Down putting fish exit to make dress fishnet pocket;

Described fish position detecting device (21) is made up of end face video camera (58) and side cameras (59), the relative program that video camera is run by server controls, the top plane view picture of end face video camera shooting fish, the side image of side cameras shooting fish, carries out judgement by Computer Image Processing algorithm to the end face of fish and lateral location and calculates.

6. fish Microexamination device as claimed in claim 5, it is characterized in that: described Fixing clamp-seat (37) installs Fixing clamp-seat spring (40) additional in Fixing clamp-seat shell (39) and gland (41) is formed, Fixing clamp-seat shell (39) is cylindrical shape, Fixing clamp-seat spring is equipped with in the inside, and it is hemispherical that gland exposes Fixing clamp-seat housing parts;

Described net grid support is used for fixing dress fishnet pocket (46), net grid support is metallic support, before and after metallic support, there are 4 symmetrical Guide rollers installing racks (52-1,52-2,52-3,52-4) side, Guide rollers installing rack has 4 Guide rollers installing holes (54-1,54-2,54-3,54-4) fixedly mount 4 Guide rollerses, dress fishnet pocket (46) is fixed on net grid support by dress fishnet pocket installing hole (53-1,53-2,53-3,53-4); Before and after metallic support, the connection of side is elasticity connecting bridge, and Guide rollers spacing can be changed with the guide rail spacing change of dress fishnet lattice; The metallic support left and right sides has 4 symmetrical connection holes (49-1,49-2,49-3,49-4) to connect adjacent mesh support;

The netted string bag is made into netted by flexible filament, two-layer, three edge sealings, and openings at one side forms a mesh bag, is arranged on string bag fixed frame; String bag fixed frame is metal frame, and three limits are single-layer metal, corresponding three limits of installing the sealing of the netted string bag; While be double-level-metal, netted string bag opening is installed; One side of the corresponding string bag opening of string bag fixed frame is flexible, and under the effect not having power, opening seals by natural resiliency; When oriented interior External Force Acting produces inside deformation, form an opening; String bag fixed frame has 4 symmetrical string bags fixed installation hole (55-1,55-2,55-3,55-4), corresponding with dress fishnet pocket installing hole (53-1,53-2,53-3,53-4);

The position one_to_one corresponding of 4 fixing holes (50-1,50-2,50-3,50-4), detection and localization hole (48) and 4 Fixing clamp-seats (37-1,37-2,37-3,37-4), detection and localization photodiode 35 and detection and localization light emitting diode 36; Make the gland of 4 Fixing clamp-seats can pass 4 fixing holes, and now, the light of detection and localization light emitting diode is irradiated on detection and localization photodiode right through detection and localization hole simultaneously.

7. fish Microexamination device as claimed in claim 1, is characterized in that: transfusion controls microprocessor-based control flow process and is:

If be separated by M dress fishnet lattice between dress fish entrance (22) and fish position detecting device (21); To be separated by between fish position detecting device (21) and infusion set (64) K dress fishnet lattice; To be separated by between infusion set (64) and microscopic image acquisition device (14) L dress fishnet lattice:

Start, the first step, by the order of communication interface reception server, second step, judges whether to start infusion program, no, returns the first step, is, the 3rd step n=0; Fish detects lumination of light emitting diode; Detection and localization lumination of light emitting diode; 4th step, controls the motor that discharges water and discharges water, the 5th step, reads fish and detects photodiode sensitization data; 6th step, judges that fish detects photosensitive reading and is less than and has fish to judge thresholding, no, returns the 5th step, is, the 7th step, and the motor that controls to discharge water closes water; Control transfer control motor to rotate to direction of transfer; 8th step, read detection and localization photodiode sensitization data, the 9th step, judges that the photosensitive reading of detection and localization is greater than location determination thresholding, no, returns the 8th step, is, the tenth step, n=n+1; Control transfer control motor stalls; Server is given by n by communication interface; 11 step, control piston pushing motor rotates, and liquid sucking device draws medicine; 12 step, judges n> (M+K+1), no, enters the 15 step, is, the 13 step, and control acupuncture treatment electromotor and rotate, pinprick enters in fish body by syringe; 14 step, control transfusion electromotor and rotate, syringe is by infusion of medicine fish body; 15 step: control transfusion device and control motor, makes syringe to move right the spacing of adjacent injectors; 16 step, by the order of communication interface reception server; 17 step, judges whether to quit work; Terminate, no, return the 4th step;

Three-shaft displacement controls microprocessor program flow process:

The first step: by communication interface reception server control data;

4th step: return the first step.

8. fish Microexamination device as claimed in claim 1, is characterized in that: the program circuit of server is: start, the first step, sends to controller order of starting working by communication interface; Second step, the injection position of setting fish; The observation place of setting fish and range of observation; X=0; 3rd step, the n value of Read Controller; 4th step, judges n=x+1, no, returns the 3rd step, is, the 5th step x=n; 6th step, n>M, no, return the 3rd step, be, the 7th step, read the view data of end face video camera and side cameras; 8th step, calculates the image edge of end face video camera and side cameras; 9th step, calculates the length of fish and wide according to the edge of end face camera review fish; The horizontal direction position of fish is determined according to the edge of end face camera review fish; The height of fish is calculated according to the edge of side cameras image of fish; The vertical direction position of fish is determined according to the edge of side cameras image of fish; Tenth step, judge n>M+K+1, no, return the 3rd step, be, 11 step, according to the injection position of the fish of the length of record K fish forward, the horizontal direction position of fish, the vertical direction position of fish and setting calculate transfusion three-shaft displacement adjusting device movable control motor, left and right moving control motor, move up and down the rotation direction and rotational angle that control motor, and calculated value is issued three-shaft displacement control microprocessor; 12 step, judge n>M+K+L+2, no, return the 3rd step, be, 13 step, according to the observation place of the fish of the length of record K+L+1 fish forward, the horizontal direction position of fish, the vertical direction position of fish and setting calculate micro-three-shaft displacement adjusting device movable control motor, left and right moving control motor, move up and down the rotation direction and rotational angle that control motor, and calculated value is issued three-shaft displacement control microprocessor; 14 step, is gathered by microscopic image acquisition device and stores micro-image; 15 step, judges whether to quit work, no, and return the 3rd step, be, the 16 step, sends to controller the order that quits work, and terminates.

9. fish Microexamination device as claimed in claim 1, is characterized in that: described server is controlled fish culture box, conveyer, infusion set by controller;

Three axle power-on and power-off machine controllers of infusing connect transfusion three axle and control electromotor up and down; Infuse about three axle left and right electric machine controllers connect three axles and control electromotor; Electric machine controller before and after three axles of infusing connects before and after transfusion three axle and controls electromotor; Micro-three axles of micro-three axle power-on and power-off machine controllers connection control electromotor, micro-three axle left and right electric machine controllers up and down and control about connecting micro-three axles to control electromotor before and after micro-three axles of electric machine controller connection before and after electromotor, micro-three axles;