CN103760373A - Cleaning device in fully-automatic chemiluminescence immunity analyzer - Google Patents
Cleaning device in fully-automatic chemiluminescence immunity analyzer Download PDFInfo
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- CN103760373A CN103760373A CN201410059897.5A CN201410059897A CN103760373A CN 103760373 A CN103760373 A CN 103760373A CN 201410059897 A CN201410059897 A CN 201410059897A CN 103760373 A CN103760373 A CN 103760373A
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Abstract
The invention relates to a cleaning device in a fully-automatic chemiluminescence immunity analyzer. The cleaning device comprises a first disk, a second disk and a cleaning piece, wherein a driving mechanism drives the first disk to rotate; a plurality of analysis containers are arranged at the peripheral part of the first disk at intervals, and magnetic microspheres used for adsorbing body fluid samples to be detected are put in the analysis containers; the second disk is coaxially arranged above a rotating disk, and the diameter of the second disk is smaller than the diameter of the first disk; a plurality of electromagnets are arranged at the peripheral part of the second disk at intervals; the cleaning piece is arranged on one side of the first disk and is opposite to at least one of the electromagnets in the second disk. The first disk rotates to drive the analysis containers on the first disk to selectively stay in the position of the cleaning piece. The cleaning device only needs one rotation disk and a plurality of electromagnets so as to realize that the magnetic microspheres in each analysis container are selectively controlled to be in an adsorption state or a non-adsorption state according to the demands, and the cleaning efficiency is greatly increased.
Description
Technical field
The present invention relates to the medicine equipment in a kind of vitro detection field, particularly relate to a kind of magnetic microsphere that adopts as the cleaning device in the Full-automatic chemiluminescence immunoassay analysis meter of reaction carriers.
Background technology
Full-automatic chemiluminescence immunoassay analysis meter is a kind of instrument that can carry out to patient's body fluid sample immunology quantitative test, can check the diseases such as anaemia, angiocardiopathy, congenital disorders, sex hormone, infectious disease, metabolic function, tumor markers, medicine monitoring and thyroid gland.The application of Full-automatic chemiluminescence immunoassay analysis meter, make checkout procedure realize robotization completely, improved the work efficiency of immunoassay, eliminated artificial subjective error, stablized the quality of immunity inspection, for clinical immunoassay provides a reliable approach efficiently.
In the Full-automatic chemiluminescence immunoassay analysis meter that adopts magnetic microsphere as reaction carriers, conventionally use multiple disposal reaction cups as reaction vessel simultaneously.These reaction cup are arranged on disk around at each interval.The object that multiple reaction cup are set is in order to improve analysis efficiency, can in different reaction cup the insides, launch the differential responses operation of the sample of different people simultaneously.And adding in reaction cup after body fluid sample to be analyzed, then contain absorption and have the micron of antigen or antibody or the reaction reagent of nano level magnetic microsphere to adding in reaction cup.Magnetic microsphere in reagent is suspended in reagent.After the incubation reaction of sample and reagent completes, need to clean, remove unreacted body fluid sample and reagent, only leave the reacted compound using magnetic microsphere as solid phase carrier.
When cleaning, pass through rotary disk, make reaction cup to be cleaned forward wiper mechanism below to, and on the opposite of wiper mechanism, be provided with permanent magnet, permanent magnet passes through magnetic fields, make magnetic microsphere to the motion of permanent magnet direction, and finally by the wall of cup of reaction cup, stopped, be finally attached to the wall of cup inner side of reaction cup near permanent magnet one side.At this time with the aspirating needle on wiper mechanism, be deep into reaction cup bottom, comprising liquid sample in reaction cup, and unreacted reagent is taken out at interior all liq.And magnetic microsphere solid phase carrier is owing to being adsorbed on wall of cup, can not be pulled away.Afterwards, wiper mechanism injects cleaning fluid by fluid injection pin again in reaction cup, and magnetic microsphere is cleaned as the compound of solid phase carrier.Afterwards cleaning fluid is extracted out, after repeatedly repeating, completed whole cleaning process.
But there is following defect in prior art: 1) when magnetic microsphere solid phase carrier is cleaned, because it is attached on wall of cup, other magnetic microspheres except magnetic microsphere layer surface are difficult to clean fully, cause cleaning insufficient.2) magnetic microsphere is suspended in liquid, even at the magnetic fields of permanent magnet, whole adsorption process needs tens of seconds conventionally, has greatly affected work efficiency.And if polylith permanent magnet is set on the rotation path of disk, although can allow the magnetic microsphere in reaction cup to be cleaned complete adsorption process in the process of disk rotation, but owing to being provided with multiple reaction cup on disk, these permanent magnets also can to other just the reaction cup in course of reaction cause magnetic interference improperly.Because in course of reaction, be to apply magnetic field, and only have absorption magnetic microsphere, and in the process of sucking-off cleaning fluid, just need to apply magnetic field.
Summary of the invention
For overcoming the deficiencies in the prior art, the object of the present invention is to provide the cleaning device in a kind of Full-automatic chemiluminescence immunoassay analysis meter, magnetic microsphere magnetic field energy when cleaning is removed, magnetic microsphere can fully be cleaned, and unlikely because being attracted on wall of cup, cause cleaning not exclusively.
Whether another object of the present invention is to provide a kind of and can completes and whether Independent Decisiveness adds loaded magnetic field to improve the cleaning device of service efficiency according to reaction in reaction cup.
For achieving the above object, the present invention is achieved through the following technical solutions:
A cleaning device in Full-automatic chemiluminescence immunoassay analysis meter, comprising:
The first disk, it is by driving mechanism driven rotary, and the peripheral part of described the first disk is provided with spaced multiple analyzing container, throws in the magnetic microsphere that is useful on absorption body fluid sample to be detected in analyzing container;
The second disk, it is arranged on the top of described the first disk coaxially, and the diameter of described the second disk is less than the diameter of described the first disk, and the peripheral part of described the second disk is provided with spaced multiple electromagnet;
Cleaning part, it is arranged on a side of described the first disk, and relative with at least one in multiple electromagnets of described the second disk;
Wherein, the first disk rotates, to drive the analyzing container on the first disk optionally to rest on cleaning part place.
Preferably, cleaning device in described Full-automatic chemiluminescence immunoassay analysis meter, described driving mechanism adopts synchronous belt drive mechanism, it comprises motor, Timing Belt, synchronous pulley, the outer of described the first disk is provided with the tooth engaging with described Timing Belt, the output terminal of described motor connects described synchronous pulley, and described synchronous pulley is connected with the tooth of described the first disk by described Timing Belt, and described motor is optionally rotated or stopped by the first disk described in Timing Belt control.
Preferably, the cleaning device in described Full-automatic chemiluminescence immunoassay analysis meter, described multiple analyzing containers interval each other equates with described multiple electromagnets interval each other.
Preferably, the cleaning device in described Full-automatic chemiluminescence immunoassay analysis meter, described cleaning part comprises:
Bracing frame, comprises base and footstock, and described footstock is provided with lifting table;
Aspirating needle, it is arranged on described lifting table, and vertically extends downwards by described lifting table;
Fluid injection pin, itself and described aspirating needle are arranged side by side on described lifting table, and extend downwards by described lifting table, and described fluid injection pin tilts towards described electromagnet direction;
Soda liquor container, itself and described fluid injection pin pass through pipeline connection, and on pipeline, are provided with liquid pump;
Delivery pipe, it is communicated with described aspirating needle, and is provided with liquid pump on pipeline.
Preferably, the cleaning device in described Full-automatic chemiluminescence immunoassay analysis meter, described analyzing container is upper opening cylinder tank, when analyzing container rests on cleaning part place, aspirating needle, fluid injection pin are relative with a tank opening.
Preferably, the cleaning device in described Full-automatic chemiluminescence immunoassay analysis meter, the whole peripheral part of described the second disk is provided with spaced multiple electromagnet, and described cleaning device also comprises:
Controller, controls separately the power on/off of each electromagnet.
Preferably, cleaning device in described Full-automatic chemiluminescence immunoassay analysis meter, when described controller control liquid pump, make in adsorption process and during aspirating needle imbibition, the electromagnet energising corresponding with analyzing container to be cleaned, and when described controller control liquid pump, while making the fluid injection of fluid injection pin, the electromagnet power-off corresponding with analyzing container to be cleaned.
Preferably, the cleaning device in described Full-automatic chemiluminescence immunoassay analysis meter, also comprises:
Timer, it calculates the reaction time of each analyzing container, and described timer is connected with described controller communication, and the reaction time of the analyzing container receiving when controller exceedes setting value, and the reaction being judged to be in this analyzing container finishes;
Steady arm, it is positioned in the process of the first dial rotation, the residing position of each analyzing container;
If controller judges the reaction in an analyzing container, finish, according to the location of steady arm, start its relative electromagnet energising; If carrying out and controller judges the reaction in an analyzing container, according to the location of steady arm, making its relative electromagnet power-off.
Preferably, the cleaning device in described Full-automatic chemiluminescence immunoassay analysis meter, described magnetic microsphere is nano-scale magnetic microballoon.
The invention has the beneficial effects as follows: the disk that 1) can only need one deck to rotate freely, simplified apparatus structure; 2) introducing of electromagnet makes magnetic field be easy to control, and when injecting cleaning liquid, can automatically remove or adjust magnetic field, and magnetic microsphere is fully cleaned; 3) can make the reaction cup of having reacted before not yet arriving cleaning part, just be subject to magnetic field suction, start adsorption process, to save the adsorption time arriving after cleaning machine, and react uncompleted reaction cup, not be subject to magnetic field suction.
Accompanying drawing explanation
Fig. 1 is the vertical view of the cleaning device in Full-automatic chemiluminescence immunoassay analysis meter of the present invention.
Embodiment
Below in conjunction with accompanying drawing, the present invention is described in further detail, to make those skilled in the art can implement according to this with reference to instructions word.
The step of the checkout procedure of Full-automatic chemiluminescence immunoassay analysis meter comprises: load reaction cup, add that sample, reagent adding, incubation reaction, cleaning separate, filling exciting liquid, luminous signal detect and testing result calculating etc.
For the Full-automatic chemiluminescence immunoassay analysis meter that adopts rotating disk transmission, according to the project organization of different product, sometimes sample reagent application of sample portion, loading part, hatching in portion, these parts of cleaning part can more public parts, as rotating disk etc.
Refer to Fig. 1, its explanation one embodiment of the invention a kind of Full-automatic chemiluminescence immunoassay analysis meter in for cleaning the cleaning device of separation, it comprises: the first disk 1; The second disk 2; Electromagnet 3; Cleaning part 4; Analyzing container 5.
The first disk 1 is rotating disc, and it is by driving mechanism driven rotary, and its peripheral part is provided with spaced multiple analyzing container 5, throws in the nano-scale magnetic microballoon that is useful on absorption body fluid sample to be detected in analyzing container 5; In this case, driving mechanism adopts synchronous belt drive mechanism, it comprises motor, Timing Belt, synchronous pulley, the outer of the first disk is provided with the tooth engaging with Timing Belt, the output terminal of motor connects synchronous pulley, synchronous pulley is connected with the tooth of the first disk by Timing Belt, and motor is optionally rotated or stopped by Timing Belt control the first disk.
The second disk 2 is shaft collars, and it is arranged on the top of the first disk 1 coaxially, and the diameter of the second disk 2 is less than the diameter of the first disk 1, and the whole peripheral part of the second disk 2 is provided with spaced multiple electromagnet 3; Multiple analyzing containers 5 interval each other equates with multiple electromagnets 3 interval each other.
Bracing frame, comprises base and footstock, and footstock is provided with lifting table;
Aspirating needle, it is arranged on lifting table, and vertically extends downwards by lifting table;
Fluid injection pin, itself and aspirating needle are arranged side by side on lifting table, and extend downwards by lifting table, and fluid injection pin tilts towards electromagnet direction;
Soda liquor container, itself and fluid injection pin pass through pipeline connection, and on pipeline, are provided with liquid pump;
Delivery pipe, it is communicated with aspirating needle, and is provided with liquid pump on pipeline.
The first disk 1 rotates, to drive the analyzing container 5 on the first disk 1 optionally to rest on cleaning part place.Analyzing container 5 is upper opening cylinder tank, when analyzing container 5 rests on cleaning part 4 place, aspirating needle, fluid injection pin are relative with a tank opening.
This cleaning device also comprises:
Controller, controls separately the power on/off of each electromagnet.When described controller control liquid pump, make in adsorption process and during aspirating needle imbibition, the electromagnet energising corresponding with analyzing container to be cleaned, and when described controller control liquid pump, while making the fluid injection of fluid injection pin, the electromagnet power-off corresponding with analyzing container to be cleaned.
Timer, it calculates the reaction time of each analyzing container, and timer is connected with controller communication, and the reaction time of the analyzing container receiving when controller exceedes setting value, and the reaction being judged to be in this analyzing container finishes;
Steady arm, it is positioned in the process of the first dial rotation, the residing position of each analyzing container;
If controller judges the reaction in an analyzing container, finish, according to the location of steady arm, start its relative electromagnet energising; If carrying out and controller judges the reaction in an analyzing container, according to the location of steady arm, making its relative electromagnet power-off.
The corresponding chemical illumination immunity analysis instrument that adopts the rotating disk kind of drive, generally in the process of wait magnetic microsphere absorption, the first disk 1 interval certain hour meeting automatic rotation, move to another working position, operate accordingly, and in rotary course, provide the individuality of the electromagnet 3 that the analyzing container 5 in magnetic field is corresponding constantly to change with need to be cleaned, by the power supply of switching electromagnet 3 individualities, reach electromagnetic field synchronous action effect relative to analyzing container 5, to guarantee its adsorption effect.
It should be noted that Fig. 1 is schematic diagram, the quantity of the electromagnet 3 in the second disk 2 can require to increase and decrease according to the difference of magnetic field synchronization accuracy.Corresponding different scheme of machine design, the effect of the second disk 2 and the first disk 1 may be exchanged, and can cause accordingly the variation of electromagnet 3 and analyzing container 5 positions.
Although embodiment of the present invention are open as above, but it is not restricted to listed utilization in instructions and embodiment, it can be applied to various applicable the field of the invention completely, for those skilled in the art, can easily realize other modification, therefore do not deviating under the universal that claim and equivalency range limit, the present invention is not limited to specific details and illustrates here and the legend of describing.
Claims (9)
1. the cleaning device in Full-automatic chemiluminescence immunoassay analysis meter, is characterized in that, comprising:
The first disk, it is by driving mechanism driven rotary, and the peripheral part of described the first disk is provided with spaced multiple analyzing container, throws in the magnetic microsphere that is useful on absorption body fluid sample to be detected in analyzing container;
The second disk, it is arranged on the top of described the first disk coaxially, and the diameter of described the second disk is less than the diameter of described the first disk, and the peripheral part of described the second disk is provided with spaced multiple electromagnet;
Cleaning part, it is arranged on a side of described the first disk, and relative with at least one in multiple electromagnets of described the second disk;
Wherein, the first disk rotates, to drive the analyzing container on the first disk optionally to rest on cleaning part place.
2. the cleaning device in Full-automatic chemiluminescence immunoassay analysis meter as claimed in claim 1, it is characterized in that, described driving mechanism adopts synchronous belt drive mechanism, it comprises motor, Timing Belt, synchronous pulley, the outer of described the first disk is provided with the tooth engaging with described Timing Belt, the output terminal of described motor connects described synchronous pulley, described synchronous pulley is connected with the tooth of described the first disk by described Timing Belt, and described motor is optionally rotated or stopped by the first disk described in Timing Belt control.
3. the cleaning device in Full-automatic chemiluminescence immunoassay analysis meter as claimed in claim 1 or 2, is characterized in that, described multiple analyzing containers interval each other equates with described multiple electromagnets interval each other.
4. the cleaning device in Full-automatic chemiluminescence immunoassay analysis meter as claimed in claim 3, is characterized in that, described cleaning part comprises:
Bracing frame, comprises base and footstock, and described footstock is provided with lifting table;
Aspirating needle, it is arranged on described lifting table, and vertically extends downwards by described lifting table;
Fluid injection pin, itself and described aspirating needle are arranged side by side on described lifting table, and extend downwards by described lifting table, and described fluid injection pin tilts towards described electromagnet direction;
Soda liquor container, itself and described fluid injection pin pass through pipeline connection, and on pipeline, are provided with liquid pump;
Delivery pipe, it is communicated with described aspirating needle, and is provided with liquid pump on pipeline.
5. the cleaning device in Full-automatic chemiluminescence immunoassay analysis meter as claimed in claim 4, is characterized in that, described analyzing container is upper opening cylinder tank, and when analyzing container rests on cleaning part place, aspirating needle, fluid injection pin are relative with a tank opening.
6. the cleaning device in Full-automatic chemiluminescence immunoassay analysis meter as claimed in claim 5, is characterized in that, the whole peripheral part of described the second disk is provided with spaced multiple electromagnet, and described cleaning device also comprises:
Controller, controls separately the power on/off of each electromagnet.
7. the cleaning device in Full-automatic chemiluminescence immunoassay analysis meter as claimed in claim 6, it is characterized in that, when described controller control liquid pump, make in adsorption process and during aspirating needle imbibition, the electromagnet energising corresponding with analyzing container to be cleaned, and when described controller control liquid pump, while making the fluid injection of fluid injection pin, the electromagnet power-off corresponding with analyzing container to be cleaned.
8. the cleaning device in Full-automatic chemiluminescence immunoassay analysis meter as claimed in claim 7, is characterized in that, also comprises:
Timer, it calculates the reaction time of each analyzing container, and described timer is connected with described controller communication, and the reaction time of the analyzing container receiving when controller exceedes setting value, and the reaction being judged to be in this analyzing container finishes;
Steady arm, it is positioned in the process of the first dial rotation, the residing position of each analyzing container;
If controller judges the reaction in an analyzing container, finish, according to the location of steady arm, start its relative electromagnet energising; If carrying out and controller judges the reaction in an analyzing container, according to the location of steady arm, making its relative electromagnet power-off.
9. the cleaning device in Full-automatic chemiluminescence immunoassay analysis meter as claimed in claim 8, is characterized in that, described magnetic microsphere is nano-scale magnetic microballoon.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201410059897.5A CN103760373B (en) | 2014-02-21 | 2014-02-21 | Cleaning device in fully-automatic chemiluminescence immunity analyzer |
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| CN201410059897.5A CN103760373B (en) | 2014-02-21 | 2014-02-21 | Cleaning device in fully-automatic chemiluminescence immunity analyzer |
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| CN103760373B CN103760373B (en) | 2015-06-10 |
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Cited By (8)
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| CN105834176A (en) * | 2016-05-20 | 2016-08-10 | 威海威高生物科技有限公司 | Convenient and fast cleaning device of full-automatic chemiluminescence tester |
| CN110488029A (en) * | 2014-06-17 | 2019-11-22 | 深圳迈瑞生物医疗电子股份有限公司 | Extract separator and its working method |
| CN111007222A (en) * | 2019-12-24 | 2020-04-14 | 安徽大学 | Device for realizing simultaneous determination of water quality indexes by multiple samples |
| CN111381058A (en) * | 2018-12-29 | 2020-07-07 | 深圳市帝迈生物技术有限公司 | Magnetic separation device, sample analyzer, and flow type fluorescence immunoassay analyzer |
| CN111381056A (en) * | 2018-12-29 | 2020-07-07 | 深圳市帝迈生物技术有限公司 | Magnetic separation device, sample analyzer, and flow type fluorescence immunoassay analyzer |
| CN114712812A (en) * | 2022-06-10 | 2022-07-08 | 南通豪天运动器材有限公司 | Negative pressure formula autogyration volleyball cleaning machine |
| CN115254834A (en) * | 2022-08-02 | 2022-11-01 | 宋世琦 | Magnetic particle cleaning device and cleaning method for immunoassay device |
| CN116786504A (en) * | 2023-08-29 | 2023-09-22 | 江苏三联生物工程股份有限公司 | Magnetic bead cleaning and separating device and method for chemiluminescent immunoassay analyzer |
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| CN116786504A (en) * | 2023-08-29 | 2023-09-22 | 江苏三联生物工程股份有限公司 | Magnetic bead cleaning and separating device and method for chemiluminescent immunoassay analyzer |
| CN116786504B (en) * | 2023-08-29 | 2023-11-03 | 江苏三联生物工程股份有限公司 | Magnetic bead cleaning and separating device and method for chemiluminescent immunoassay analyzer |
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Application publication date: 20140430 Assignee: Suzhou Guoke Junhao Biotechnology Co., Ltd. Assignor: Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences Contract record no.: 2018320010060 Denomination of invention: Cleaning device in fully-automatic chemiluminescence immunity analyzer Granted publication date: 20150610 License type: Exclusive License Record date: 20181126 |
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