CN103751351A - Preparation method of Qingmei cold-treatment tablet and use of Qingmei cold-treatment tablet in drug for inhibiting Leydig cell tumor cell MLTC-1 proliferation - Google Patents
Preparation method of Qingmei cold-treatment tablet and use of Qingmei cold-treatment tablet in drug for inhibiting Leydig cell tumor cell MLTC-1 proliferation Download PDFInfo
- Publication number
- CN103751351A CN103751351A CN201310660198.1A CN201310660198A CN103751351A CN 103751351 A CN103751351 A CN 103751351A CN 201310660198 A CN201310660198 A CN 201310660198A CN 103751351 A CN103751351 A CN 103751351A
- Authority
- CN
- China
- Prior art keywords
- preparation
- extraction
- common cold
- armeniaca mume
- mume sieb
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000002360 preparation method Methods 0.000 title claims abstract description 35
- 239000003814 drug Substances 0.000 title claims abstract description 23
- 229940079593 drug Drugs 0.000 title claims abstract description 15
- 230000002401 inhibitory effect Effects 0.000 title abstract description 4
- 201000004462 Leydig Cell Tumor Diseases 0.000 title abstract 2
- 230000035755 proliferation Effects 0.000 title abstract 2
- 210000004881 tumor cell Anatomy 0.000 title abstract 2
- 238000000194 supercritical-fluid extraction Methods 0.000 claims abstract description 16
- 238000000874 microwave-assisted extraction Methods 0.000 claims abstract description 11
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 53
- 235000011158 Prunus mume Nutrition 0.000 claims description 42
- 244000018795 Prunus mume Species 0.000 claims description 42
- 201000009240 nasopharyngitis Diseases 0.000 claims description 34
- 238000000605 extraction Methods 0.000 claims description 22
- 239000000284 extract Substances 0.000 claims description 16
- 239000008187 granular material Substances 0.000 claims description 15
- 239000007788 liquid Substances 0.000 claims description 9
- 230000004663 cell proliferation Effects 0.000 claims description 7
- 241000628997 Flos Species 0.000 claims description 6
- 229940126678 chinese medicines Drugs 0.000 claims description 6
- 210000002332 leydig cell Anatomy 0.000 claims description 6
- 229920002472 Starch Polymers 0.000 claims description 5
- 230000000274 adsorptive effect Effects 0.000 claims description 5
- 239000012141 concentrate Substances 0.000 claims description 5
- 230000006837 decompression Effects 0.000 claims description 5
- 239000003480 eluent Substances 0.000 claims description 5
- 238000010828 elution Methods 0.000 claims description 5
- 238000004064 recycling Methods 0.000 claims description 5
- 229920005989 resin Polymers 0.000 claims description 5
- 239000011347 resin Substances 0.000 claims description 5
- 235000019698 starch Nutrition 0.000 claims description 5
- 239000008107 starch Substances 0.000 claims description 5
- VTAJIXDZFCRWBR-UHFFFAOYSA-N Licoricesaponin B2 Natural products C1C(C2C(C3(CCC4(C)CCC(C)(CC4C3=CC2)C(O)=O)C)(C)CC2)(C)C2C(C)(C)CC1OC1OC(C(O)=O)C(O)C(O)C1OC1OC(C(O)=O)C(O)C(O)C1O VTAJIXDZFCRWBR-UHFFFAOYSA-N 0.000 abstract description 11
- LPLVUJXQOOQHMX-UHFFFAOYSA-N glycyrrhetinic acid glycoside Natural products C1CC(C2C(C3(CCC4(C)CCC(C)(CC4C3=CC2=O)C(O)=O)C)(C)CC2)(C)C2C(C)(C)C1OC1OC(C(O)=O)C(O)C(O)C1OC1OC(C(O)=O)C(O)C(O)C1O LPLVUJXQOOQHMX-UHFFFAOYSA-N 0.000 abstract description 11
- 239000001685 glycyrrhizic acid Substances 0.000 abstract description 11
- 229960004949 glycyrrhizic acid Drugs 0.000 abstract description 11
- UYRUBYNTXSDKQT-UHFFFAOYSA-N glycyrrhizic acid Natural products CC1(C)C(CCC2(C)C1CCC3(C)C2C(=O)C=C4C5CC(C)(CCC5(C)CCC34C)C(=O)O)OC6OC(C(O)C(O)C6OC7OC(O)C(O)C(O)C7C(=O)O)C(=O)O UYRUBYNTXSDKQT-UHFFFAOYSA-N 0.000 abstract description 11
- 235000019410 glycyrrhizin Nutrition 0.000 abstract description 11
- LPLVUJXQOOQHMX-QWBHMCJMSA-N glycyrrhizinic acid Chemical compound O([C@@H]1[C@@H](O)[C@H](O)[C@H](O[C@@H]1O[C@@H]1C([C@H]2[C@]([C@@H]3[C@@]([C@@]4(CC[C@@]5(C)CC[C@@](C)(C[C@H]5C4=CC3=O)C(O)=O)C)(C)CC2)(C)CC1)(C)C)C(O)=O)[C@@H]1O[C@H](C(O)=O)[C@@H](O)[C@H](O)[C@H]1O LPLVUJXQOOQHMX-QWBHMCJMSA-N 0.000 abstract description 11
- 229940126680 traditional chinese medicines Drugs 0.000 abstract description 2
- 235000001405 Artemisia annua Nutrition 0.000 abstract 1
- 240000000011 Artemisia annua Species 0.000 abstract 1
- 240000007126 Citrus medica var. sarcodactylis Species 0.000 abstract 1
- 244000303040 Glycyrrhiza glabra Species 0.000 abstract 1
- 235000006200 Glycyrrhiza glabra Nutrition 0.000 abstract 1
- 235000017443 Hedysarum boreale Nutrition 0.000 abstract 1
- 235000007858 Hedysarum occidentale Nutrition 0.000 abstract 1
- 241000209035 Ilex Species 0.000 abstract 1
- 235000003332 Ilex aquifolium Nutrition 0.000 abstract 1
- 235000002296 Ilex sandwicensis Nutrition 0.000 abstract 1
- 235000002294 Ilex volkensiana Nutrition 0.000 abstract 1
- 241000717679 Mosla Species 0.000 abstract 1
- 241001090408 Strobilanthes Species 0.000 abstract 1
- 239000001947 glycyrrhiza glabra rhizome/root Substances 0.000 abstract 1
- 229960004756 ethanol Drugs 0.000 description 20
- 210000004027 cell Anatomy 0.000 description 16
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 15
- 238000000034 method Methods 0.000 description 11
- 238000002474 experimental method Methods 0.000 description 8
- 239000000243 solution Substances 0.000 description 8
- 238000012360 testing method Methods 0.000 description 7
- 239000000047 product Substances 0.000 description 6
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 4
- 239000013558 reference substance Substances 0.000 description 4
- 239000006144 Dulbecco’s modified Eagle's medium Substances 0.000 description 3
- 230000010261 cell growth Effects 0.000 description 3
- 239000003153 chemical reaction reagent Substances 0.000 description 3
- 239000012531 culture fluid Substances 0.000 description 3
- 229960000935 dehydrated alcohol Drugs 0.000 description 3
- 239000006228 supernatant Substances 0.000 description 3
- 230000001629 suppression Effects 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 2
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 2
- 239000006285 cell suspension Substances 0.000 description 2
- 239000000706 filtrate Substances 0.000 description 2
- 239000012528 membrane Substances 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 238000003860 storage Methods 0.000 description 2
- 235000019640 taste Nutrition 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- WEEMDRWIKYCTQM-UHFFFAOYSA-N 2,6-dimethoxybenzenecarbothioamide Chemical compound COC1=CC=CC(OC)=C1C(N)=S WEEMDRWIKYCTQM-UHFFFAOYSA-N 0.000 description 1
- 206010011224 Cough Diseases 0.000 description 1
- 238000002965 ELISA Methods 0.000 description 1
- 206010019233 Headaches Diseases 0.000 description 1
- 241000167880 Hirundinidae Species 0.000 description 1
- ILRKKHJEINIICQ-OOFFSTKBSA-N Monoammonium glycyrrhizinate Chemical compound N.O([C@@H]1[C@@H](O)[C@H](O)[C@H](O[C@@H]1O[C@H]1CC[C@]2(C)[C@H]3C(=O)C=C4[C@@H]5C[C@](C)(CC[C@@]5(CC[C@@]4(C)[C@]3(C)CC[C@H]2C1(C)C)C)C(O)=O)C(O)=O)[C@@H]1O[C@H](C(O)=O)[C@@H](O)[C@H](O)[C@H]1O ILRKKHJEINIICQ-OOFFSTKBSA-N 0.000 description 1
- 206010028748 Nasal obstruction Diseases 0.000 description 1
- 229920002334 Spandex Polymers 0.000 description 1
- 102000004142 Trypsin Human genes 0.000 description 1
- 108090000631 Trypsin Proteins 0.000 description 1
- 239000002250 absorbent Substances 0.000 description 1
- 230000002745 absorbent Effects 0.000 description 1
- 229960000583 acetic acid Drugs 0.000 description 1
- 235000019257 ammonium acetate Nutrition 0.000 description 1
- 230000001754 anti-pyretic effect Effects 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 230000010165 autogamy Effects 0.000 description 1
- FCPVYOBCFFNJFS-LQDWTQKMSA-M benzylpenicillin sodium Chemical compound [Na+].N([C@H]1[C@H]2SC([C@@H](N2C1=O)C([O-])=O)(C)C)C(=O)CC1=CC=CC=C1 FCPVYOBCFFNJFS-LQDWTQKMSA-M 0.000 description 1
- 235000019658 bitter taste Nutrition 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 238000010219 correlation analysis Methods 0.000 description 1
- 239000013078 crystal Substances 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 239000012738 dissolution medium Substances 0.000 description 1
- 231100000673 dose–response relationship Toxicity 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000000945 filler Substances 0.000 description 1
- 239000012362 glacial acetic acid Substances 0.000 description 1
- 231100000869 headache Toxicity 0.000 description 1
- 238000004128 high performance liquid chromatography Methods 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 238000004900 laundering Methods 0.000 description 1
- 230000031700 light absorption Effects 0.000 description 1
- 238000004811 liquid chromatography Methods 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 238000006386 neutralization reaction Methods 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- YTJSFYQNRXLOIC-UHFFFAOYSA-N octadecylsilane Chemical compound CCCCCCCCCCCCCCCCCC[SiH3] YTJSFYQNRXLOIC-UHFFFAOYSA-N 0.000 description 1
- 230000010355 oscillation Effects 0.000 description 1
- 239000003507 refrigerant Substances 0.000 description 1
- 239000000377 silicon dioxide Substances 0.000 description 1
- 239000004759 spandex Substances 0.000 description 1
- 238000010561 standard procedure Methods 0.000 description 1
- 229960002385 streptomycin sulfate Drugs 0.000 description 1
- 238000009923 sugaring Methods 0.000 description 1
- 201000008321 testicular Leydig cell tumor Diseases 0.000 description 1
- 239000012588 trypsin Substances 0.000 description 1
- 239000000341 volatile oil Substances 0.000 description 1
Landscapes
- Medicines Containing Plant Substances (AREA)
Abstract
Description
Group | Drug level (mg/ml) | Suppression ratio (%) |
Matched group | 0 | 0 |
1 | 5 | 10.01±6.26 |
2 | 10 | 19.68±13.12* |
3 | 15 | 35.19±17.64** |
4 | 20 | 48.61±15.22** |
Claims (9)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201310660198.1A CN103751351B (en) | 2013-12-06 | 2013-12-06 | A kind of preparation method of Armeniaca mume Sieb. common cold tablet and the application in suppression leydig cell tumor of testis cell MLTC-1 cell proliferation thereof |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201310660198.1A CN103751351B (en) | 2013-12-06 | 2013-12-06 | A kind of preparation method of Armeniaca mume Sieb. common cold tablet and the application in suppression leydig cell tumor of testis cell MLTC-1 cell proliferation thereof |
Publications (2)
Publication Number | Publication Date |
---|---|
CN103751351A true CN103751351A (en) | 2014-04-30 |
CN103751351B CN103751351B (en) | 2016-01-20 |
Family
ID=50518778
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201310660198.1A Expired - Fee Related CN103751351B (en) | 2013-12-06 | 2013-12-06 | A kind of preparation method of Armeniaca mume Sieb. common cold tablet and the application in suppression leydig cell tumor of testis cell MLTC-1 cell proliferation thereof |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN103751351B (en) |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20040105902A1 (en) * | 2002-12-02 | 2004-06-03 | Jiafang Chen | Compositions and methods for treating prostate cancer |
CN102836205A (en) * | 2012-09-18 | 2012-12-26 | 南京正亮医药科技有限公司 | Preparation method of Zhilining |
CN102973667A (en) * | 2012-10-15 | 2013-03-20 | 李正梅 | Preparation method and application of cold-fever tablet |
CN102988545A (en) * | 2012-10-08 | 2013-03-27 | 卞毓平 | Preparation method and application of Yulian tablet |
-
2013
- 2013-12-06 CN CN201310660198.1A patent/CN103751351B/en not_active Expired - Fee Related
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20040105902A1 (en) * | 2002-12-02 | 2004-06-03 | Jiafang Chen | Compositions and methods for treating prostate cancer |
CN102836205A (en) * | 2012-09-18 | 2012-12-26 | 南京正亮医药科技有限公司 | Preparation method of Zhilining |
CN102988545A (en) * | 2012-10-08 | 2013-03-27 | 卞毓平 | Preparation method and application of Yulian tablet |
CN102973667A (en) * | 2012-10-15 | 2013-03-20 | 李正梅 | Preparation method and application of cold-fever tablet |
Non-Patent Citations (5)
Title |
---|
周宜强等: "《抗癌中草药》", 31 January 2008, 化学工业出版社 * |
国家药品监督管理局: "《国家药品标准(试行)颁布件》", 16 November 2002 * |
梅全喜: "《广东地产药材研究》", 31 May 2011, 广东科技出版社 * |
程剑华: "《抗癌植物药与其验方》", 30 April 1998, 江西科学技术出版社 * |
范文昌等: "《广东地产清热解毒药物大全》", 31 July 2011, 中医古籍出版社 * |
Also Published As
Publication number | Publication date |
---|---|
CN103751351B (en) | 2016-01-20 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN103655843A (en) | Yikangbuyuan tablet, and preparation method and application thereof | |
CN103800498B (en) | A kind of preparation method of Armeniaca mume Sieb. common cold tablet and the application in suppression row adenocarcinoma cell RM-1 cell proliferation thereof | |
CN103768211B (en) | A kind of preparation method of Armeniaca mume Sieb. common cold tablet and the application in suppression lymphoma cell EL4 cell proliferation thereof | |
CN103800499A (en) | Preparation method of Qingmei cold tablet and application of Qingmei cold tablet in drugs for inhibiting melanoma cell B16 from cell proliferation | |
CN103768266A (en) | Preparation method of Qingmei cold tablet and application of Qingmei cold tablet in drugs for inhibiting mouse leukaemia cell L6565 from cell proliferation | |
CN103768267A (en) | Preparation method of Qingmei cold tablet and application of Qingmei cold tablet in drugs for inhibiting lung cancer cell H22 from cell proliferation | |
CN103768262A (en) | Preparation method of Qingmei cold tablet and application of Qingmei cold tablet in drugs for inhibiting myeloma cell FO from cell proliferation | |
CN103751424A (en) | Preparation method of cold tablet containing Artemisia carvifolia and holly root, and application of cold tablet in teratoma cell F9 cell proliferation inhibition medicines. | |
CN103768263A (en) | Preparation method of Qingmei cold tablet and application of Qingmei cold tablet in drugs for inhibiting cerebral nerve oncocyte Neuro-2a from cell proliferation | |
CN103893411A (en) | Preparation method and application of green plum cold tablets | |
CN103751350B (en) | A kind of preparation method of Armeniaca mume Sieb. common cold tablet and the application in suppression leukaemia L1210 cell proliferation thereof | |
CN103768207B (en) | A kind of preparation method of Armeniaca mume Sieb. common cold tablet and the application in suppression hepatoma carcinoma cell Hepa1-6 cell proliferation thereof | |
CN103735960A (en) | Application of boenninghausenia sessilicarpa cough-asthma tablet to prepare medicaments for inhibiting lung cancer cell LLC proliferation | |
CN103768212B (en) | The preparation method of a kind of Armeniaca mume Sieb. common cold tablet and the application in suppressing stomach cancer cell MFC cell proliferation thereof | |
CN103751425A (en) | Preparation method of Qingmei cold-treatment tablet and use of Qingmei cold-treatment tablet in drug for inhibiting breast tumor cell C127 proliferation | |
CN103800569A (en) | Preparation method of Qingmei cold tablet and application of Qingmei cold tablet in drugs for inhibiting lymphoma cell YAC-1 from cell proliferation | |
CN103768265A (en) | Preparation method of Qingmei cold tablet and application of Qingmei cold tablet in drugs for inhibiting lung cancer cell LLC from cell proliferation | |
CN103768264A (en) | Preparation method of Qingmei cold tablet and application of Qingmei cold tablet in drugs for inhibiting teratoma cell P19 from cell proliferation | |
CN103751351B (en) | A kind of preparation method of Armeniaca mume Sieb. common cold tablet and the application in suppression leydig cell tumor of testis cell MLTC-1 cell proliferation thereof | |
CN103768210B (en) | A kind of preparation method of Armeniaca mume Sieb. common cold tablet and the application in suppression anus sarcoma cell S-180 cell proliferation thereof | |
CN103768209B (en) | A kind of preparation method of Armeniaca mume Sieb. common cold tablet and the application in suppression myeloma cell P3X63AG8 cell proliferation thereof | |
CN103800770A (en) | Application of boenninghausenia cough tablet in preparing medicine for inhibiting cell proliferation of breast tumor cell C127 | |
CN103751426A (en) | Preparation method of Qingmei cold-treatment tablet and use of Qingmei cold-treatment tablet in drug for inhibiting ascitic tumor cell SAC-IIB2 proliferation | |
CN103768208A (en) | Preparation method of Qingmei cold tablet and application of Qingmei cold tablet in drugs for inhibiting lymphoid cancer cell P388D1 from proliferation | |
CN103768261A (en) | Preparation method of Qingmei cold tablet and application of Qingmei cold tablet in drugs for inhibiting myeloma cell SP2/0 from cell proliferation |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
C06 | Publication | ||
PB01 | Publication | ||
C10 | Entry into substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
C41 | Transfer of patent application or patent right or utility model | ||
CB03 | Change of inventor or designer information |
Inventor after: Ma Yan Inventor after: Li Honghua Inventor after: Song Yun Inventor after: Hou Leilei Inventor before: Sun Bo |
|
COR | Change of bibliographic data | ||
TA01 | Transfer of patent application right |
Effective date of registration: 20151225 Address after: 9 the first people's Hospital of Qingdao Development Zone, Huangpu Road, Shandong, Qingdao 266555, China Applicant after: Ma Yan Address before: 250001 Shandong Province, Ji'nan City District Court building 2303 No. 288 Applicant before: JINAN XINQIDIAN MEDICINE SCIENCE & TECHNOLOGY CO., LTD. |
|
C14 | Grant of patent or utility model | ||
GR01 | Patent grant | ||
CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20160120 Termination date: 20171206 |
|
CF01 | Termination of patent right due to non-payment of annual fee |