CN103749490B - Plant virus resistant agent and preparation method thereof - Google Patents

Abstract

The invention discloses a plant virus resistant agent. The effective constituent of the prepared plant virus resistant agent is corilagin, and auxiliaries are assisted to prepare soluble concentrate, aqueous solution and microemulsion. The plant virus resistant agent is harmless to human, livestock, natural enemies of pests and other beneficial organisms, has good environment compatibility, has no residual hazard after use, has abundant resources, simple preparation method and low cost, and can be used for preventing plant virus diseases such as tobacco mosaic virus, cucumber mosaic virus and potato virus on tobacco, chili, tomato, cucurbita pepo, potato and other crops.

Description

A kind of anti-plant virus agent and preparation method thereof

Technical field

The invention belongs to plant and application thereof, be specifically related to a kind of anti-plant virus agent and the application that contain corilagin.

Background technology

Plant virus a kind ofly infects active cytozoicus pathogen by having of forming of nucleic acid and protein, and according to the 8th report of ICTV (ICTV), the plant virus kind that the current whole world finds reaches 1122.Phytopathy caused by plant virus have the title of " plant cancer ", and it is only second to the mycosis original to the extent of injury of crops, and almost often kind of crops are all subject to 2 ~ 3 kinds of viral harm.The loss that the whole world is caused by plant virus every year about 400 hundred million dollars, only the harm that causes of tobacco mosaic virus (Tobacco mosaic virus, TMV) is just more than 100,000,000 dollars.

Because plant virus has obligate parasitism to plant cell, the matter and energy place required for virus replication is provided by host plant completely, causes the metabolism of virus and host to combine together; In addition plant lacks complete immune system, and virus, once infect plant, just can be survived, until host is dead indefinite duration in plant cell.Therefore, the medicine of control of plant virosis is only difficult to optionally challenge virus and do not injure host cell, causes the anti-virus formulation research that screening is efficient, single-minded, safe at present to face great difficulty.

At present except taking traditional Anti-virus Disease Breeding, attenuated strain system cross protection, improvement culture technique, insecticide to control to pass except the method such as virus mediator and transgenosis, scientific research personnel also investigated the chemical prevention and control method of some viroses of plant.But up to the present, only have sub-fraction anti-plant virus agent to obtain practical application, these preparations are greatly mainly with putting prevention first, and how undesirable drug effect is.Due to people to antivirotic research in medicine or veterinary drug comparatively early, some anti-plant virus agents (as agricultural virazole) are exactly develop from anti-human or zoosis toxic agent.Therefore, further investigated Antiphytoviral natural materials, is conducive to the research of anti-plant virus agent, exploitation and application.

Corilagin (corilagin; Cor), 1-acyl-3,6-hexahydroxy biphenyl diformyl glucose; to tan element also known as Ke Zici; corilagin, (material) mysorethorn of tanning essence, belongs to natural plant polyphenols tannic acid compounds; for white needles powder; be soluble in methyl alcohol, ethanol, acetone, DMSO, in cold water, solvability is little, and molecular formula is C ₂₇h ₂₂o ₁₈, molecular weight 634.46, is the active ingredient in the plants such as Common Leafflower Herb, geranium wilfordii, matsumura leafflower herb, Bai Sancao, is also present in the plants such as emblic, longan, olive.

Research shows, corilagin have antitumor, anti-oxidant, antiatherosclerosis, anti-fibrinolytic, hypotensive, suppress viral, antibacterial, the biologic activity widely such as anti-inflammatory, there is very large medical value, wide market, has become one of active skull cap components of hot research in recent years.And the research of corilagin in Antiphytoviral effect is not reported so far, also await further exploitation in application agriculturally.

Summary of the invention

The object of the invention is to, there is provided a kind of anti-plant virus agent, this anti-plant virus agent can be used in tobacco, capsicum, tomato, custard squash, potatoes and other crops prevent and treat the various plants virus diseases such as tobacco mosaic virus disease, Cucumber Mosaic Virus and potato virus disease.

In order to realize above-mentioned task, the present invention adopts following technical solution:

A kind of anti-plant virus agent, this obtained anti-plant virus agent active ingredient is corilagin and adds auxiliary agent to process, and dosage form is soluble concentrate, aqua or microemulsion; Wherein:

Described soluble concentrate is prepared by following raw materials by weight:

Corilagin: 1% ~ 30%, emulsifier: 5% ~ 30%, surplus is organic solvent, and the percentage by weight sum of raw material is 100%;

Described aqua is prepared by following raw materials by weight:

Corilagin: 1% ~ 30%, emulsifier: 5% ~ 30%, surplus is water, and the percentage by weight sum of raw material is 100%;

Described microemulsion is prepared by following raw materials by weight:

Corilagin: 1% ~ 30%, organic solvent: 25% ~ 35%, emulsifier: 5% ~ 30%, surplus is water, and the percentage by weight sum of raw material is 100%.

Described organic solvent is the mixture of one or more in ethyl acetate, cyclohexanone, methyl oleate, ethanol, methyl alcohol, acetone, DMF;

Described emulsifier is the mixture of one or more in alkyl benzene sulfonate, alkylphenol polyoxyethylene class, multi-styrene phenol APEO, phenethyl phenol polyethenoxy ethers, phenethyl phenol polyethenoxy ether polyoxypropylene ethers, this Pan's series, tween, agricultural anion-phosphate.

Plant antiviral agent made by the present invention has the following advantages:

1, significantly active, can be used for tobacco, capsicum, tomato, custard squash, potatoes and other crops prevent and treat the various plants virus diseases such as tobacco mosaic virus disease, Cucumber Mosaic Virus and potato virus disease;

2, to people, animal, pest natural enemy and other beneficial organism safety, environment compatibility is good;

3, biogenic pesticide, uses rear noresidue to poison;

4, aboundresources, preparation method are simple, with low cost, suitable promotes the use of.

Embodiment

Plant antiviral agent of the present invention is active substance with corilagin, adds certain proportion auxiliary agent and carries out formulation, can obtain environment-friendly type preparation, be i.e. microemulsion, aqua or soluble concentrate.

In order to better understand essence of an invention, describe the technology contents of invention below in detail by embodiment, but invention is not limited to these embodiments.

The preparation of embodiment 1:1% corilagin microemulsion

Take corilagin 1kg, be dissolved in 30kg ethyl acetate, then add 15kg calcium dodecyl benzene sulfonate, 5kg styrene acid APEO, mixes under high velocity agitation, is heated to about 40 DEG C; Drip deionized water polishing under high-speed stirred to 100kg, drip in the process of deionized water, make temperature remain on about 40 DEG C; Water dropwises, and is warming up to 50 DEG C, stirs 1 hour, obtained 1% corilagin microemulsion 100kg.The stability, outward appearance etc. of preparation meet the requirement of commercial preparation.

The preparation of embodiment 2:30% corilagin microemulsion

Take corilagin 30kg, be dissolved in 30kg ethyl acetate, then add 15kg calcium dodecyl benzene sulfonate, 5kg styrene acid APEO, mixes under high velocity agitation, is heated to about 40 DEG C; Drip deionized water polishing under high-speed stirred to 100kg, drip in the process of deionized water and make temperature remain on about 40 DEG C; Water dropwises, and is warming up to 50 DEG C, stirs 1 hour, obtained 30% corilagin microemulsion 100kg.The stability, outward appearance etc. of preparation meet the requirement of commercial preparation.

The preparation of embodiment 3:1% corilagin soluble concentrate

Take corilagin 1kg, be dissolved in 30kg ethyl acetate, add 15kg calcium dodecyl benzene sulfonate again, 5kg styrene acid APEO, ethanol polishing is to 100kg, after mixing, stir 10 ~ 30 minutes with mixing speed 800 ~ 1000 revs/min, 1% corilagin soluble concentrate 100kg can be obtained.The stability, outward appearance etc. of preparation meet the requirement of commercial preparation.

The preparation of embodiment 4:30% corilagin soluble concentrate

Take corilagin 30kg, be dissolved in 30kg ethyl acetate, add 15kg calcium dodecyl benzene sulfonate again, 5kg styrene acid APEO, ethanol polishing is to 100kg, after mixing, stir 10 ~ 30 minutes with mixing speed 800 ~ 1000 revs/min, corilagin 30% soluble concentrate 100kg can be obtained.The stability, outward appearance etc. of preparation meet the requirement of commercial preparation.

The preparation of embodiment 5:1% corilagin aqua

Take corilagin 1kg, be dissolved in 30kg water, then add 15kg calcium dodecyl benzene sulfonate, 5kg styrene acid APEO, water polishing to 100kg, after mixing, stir 10 ~ 30 minutes with mixing speed 800 ~ 1000 revs/min, corilagin 1% aqua 100kg can be obtained.The stability, outward appearance etc. of preparation meet the requirement of commercial preparation.

The preparation of embodiment 6:30% corilagin aqua

Take corilagin 30kg, be dissolved in 30kg water, add 15kg calcium dodecyl benzene sulfonate again, 5kg styrene acid APEO, water polishing is to 100kg, after mixing, stir 10 ~ 30 minutes with mixing speed 800 ~ 1000 revs/min, corilagin 30% soluble concentrate 100kg can be obtained.The stability, outward appearance etc. of preparation meet the requirement of commercial preparation.

It should be noted that, above-mentioned organic solvent, emulsifier are not limited to the example in embodiment, and such as, organic solvent selects the mixture of one or more in cyclohexanone, methyl oleate, ethanol, methyl alcohol, acetone, DMF; Emulsifier selects the mixture of one or more in alkyl benzene sulfonate, alkylphenol polyoxyethylene class, multi-styrene phenol APEO, phenethyl phenol polyethenoxy ethers, phenethyl phenol polyethenoxy ether polyoxypropylene ethers, this Pan's series, tween, agricultural anion-phosphate can prepare qualified commercial preparation.

The 1% corilagin microemulsion that above-described embodiment 1 ~ 6 obtains, 30% corilagin microemulsion, 1% corilagin soluble concentrate, 30% corilagin soluble concentrate, 1% corilagin aqua and 30% corilagin aqua, test through inventor proves, has good control efficiency to tobacco mosaic virus (TMV).

Embodiment 7:6 kind corilagin antivirotic is to the indoor bioassay of TMV

(1) to the external passivation effect of TMV

Adopt the withered spot method of half leaf to measure, choosing vigorous, that growing way is consistent 5 ~ 6 leaf phase Nicotiana glutinosas (Nicotiana glutinosa) of growth is withered spot host.Take vein as boundary, Zuo Banye inoculates liquid and viral equal-volume mixed liquor, and right half leaf inoculation distilled water and viral equal-volume mixed liquor compare, and virus inoculation concentration is 10 μ g/mL, and passivation time is 5 min, rinses inoculation blade surface immediately after inoculation with clear water.Often process inoculation 4 leaves, test repetition 3 times, adds up withered spot number after 3d, calculates inhibiting rate.

Inhibiting rate (%)=(contrast withered spot number-process withered spot number)/contrast withered spot number × 100

Measurement result is in table 1.

Table 1:6 kind corilagin antivirotic is to TMV inactivation in vitro

Reagent agent	Passivation time/(min)	Extension rate/doubly	Inhibiting rate/%
				30% corilagin microemulsion	5	200	95.83
1% corilagin microemulsion	5	200	76.60
				30% corilagin aqua	5	200	94.69
1% corilagin aqua	5	200	71.83

30% corilagin soluble concentrate	5	200	96.88
				1% corilagin soluble concentrate	5	200	74.12
20% moroxydine. second copper wetting powder	5	200	36.12

(2) to the inhibition of TMV First aggression

Adopt the withered spot method of half leaf, 5 ~ 6 leaf phase Nicotiana glutinosas choosing growing way consistent, healthy are withered spot host, virus inoculation after smearing liquid 6h, 12h, 24h, blank is clear water process, and 4 leaves are inoculated in each process, repeats 3 times, add up withered spot number after 3d, calculate inhibiting rate.The results are shown in Table 2.

Table 2:6 kind corilagin antivirotic is to the inhibition of TMV First aggression

(3) to the inhibition of TMV propagation

Adopt the withered spot method of half leaf, 5 ~ 6 leaf phase Nicotiana glutinosas choosing growing way consistent, healthy are withered spot host, after virus inoculation 6h, 12h, 24h, smear liquid, blank is clear water process, and 4 leaves are inoculated in each process, repeats 3 times, add up withered spot number after 3d, calculate inhibiting rate.The results are shown in Table 3.

The inhibition that table 3:6 kind corilagin antivirotic is bred TMV

From table 1,2,3,6 kinds of corilagin antivirotics are to the passivation of TMV, and First aggression, proliferation inhibiting effect are good, apparently higher than contrast medicament moroxydine. second copper.

Although above the present invention is described in detail with a general description of the specific embodiments, on basis of the present invention, can make some modifications or improvements it, this will be apparent to those skilled in the art.Therefore, these modifications or improvements without departing from theon the basis of the spirit of the present invention, all belong to protection scope of the present invention.

Claims (3)

1. an anti-plant virus agent, is characterized in that, this obtained anti-plant virus agent is main component by corilagin and is added auxiliary agent to process, and dosage form is soluble concentrate, aqua or microemulsion; Wherein:

Described soluble concentrate is prepared by following raw materials by weight:

Corilagin: 1% ~ 30%, emulsifier: 5% ~ 30%, surplus is organic solvent, and the percentage by weight sum of raw material is 100%;

Described aqua is prepared by following raw materials by weight:

Corilagin: 1% ~ 30%, emulsifier: 5% ~ 30%, surplus is water, and the percentage by weight sum of raw material is 100%;

Described microemulsion is prepared by following raw materials by weight:

Corilagin: 1% ~ 30%, organic solvent: 25% ~ 30%, emulsifier: 5% ~ 30%, surplus is water, and the percentage by weight sum of raw material is 100%.

2. anti-plant virus agent as claimed in claim 1, is characterized in that:

Described organic solvent is the mixture of one or more in ethyl acetate, cyclohexanone, methyl oleate, ethanol, methyl alcohol, acetone, DMF;

Described emulsifier is the mixture of one or more in alkyl benzene sulfonate, alkylphenol polyoxyethylene class, multi-styrene phenol APEO, phenethyl phenol polyethenoxy ethers, phenethyl phenol polyethenoxy ether polyoxypropylene ethers, this Pan's series, tween, agricultural anion-phosphate.

3. the anti-plant virus agent described in claim 1 or 2 is for the preparation of the application preventing and treating viroses of plant agricultural chemicals.