CN103740594A - Preparation method of chlorella cream - Google Patents
Preparation method of chlorella cream Download PDFInfo
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- CN103740594A CN103740594A CN201410031517.7A CN201410031517A CN103740594A CN 103740594 A CN103740594 A CN 103740594A CN 201410031517 A CN201410031517 A CN 201410031517A CN 103740594 A CN103740594 A CN 103740594A
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- chlorella
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Abstract
The invention discloses a preparation method of chlorella cream, belonging to the field of concentration and purification of living microalgae. The method mainly comprises the following steps of filtration, microfiltration/ultrafiltration and centrifugation, and is particularly applicable to preparing the chlorella cream; the prepared chlorella cream has high purity and high concentration efficiency; the problem that a flocculating agent is remained does not exist; the quality is stable, the content of chlorella is high and the preservation time is long.
Description
Technical field
The present invention relates to a kind of preparation method of chlorella cream, particularly relate to a kind of based on filtering, the preparation method of concentrated, centrifugal chlorella cream, belong to the concentrated and purified field of the micro-algae of live body.
Background technology
Chlorella (Chlorella) is the general natural disposition unicell green alga of Chlorophyta Chlorella, it is a kind of spherical unicellular fresh water algae, 3~8 microns of diameters, one of life the earliest on the earth, appeared at before more than 20 hundred million years, gene does not change all the time, is a kind of efficient photosynthetic plant, with photoautotrophy growth and breeding, distributed pole is wide.Common chlorella has Chlorella pyrenoidesa, the raw chlorella of salt, eyespot chlorella, avette chlorella and sea growing chlorella.To breed like spore, it is the only reproduction type of kind in Chlorella.
Chlorella contains rich in protein, VITAMIN, mineral substance, foodstuff fibre, nucleic acid and chlorophyll etc., be the indispensable nutrient substance that maintains and promote health, particularly contain compelling biologically active substance glycoprotein, polysaccharide body and up to materials such as 13% nucleic acid.There is the human immunity of enhancing, prevent virus multiplication, anticancer propagation, suppress blood sugar and rise, reduce serum cholesterol content, get rid of toxin, repair rapidly the function such as damage of body.
Because chlorella contains abundant nutritive substance, therefore of many uses, be often used in following aspect:
1) nutritional additive--protein, chlorophyll, VITAMIN, mineral substance, the nucleic acid of high-content are provided;
2) as food dye extract;
3) feed-fishes and shrimps open-mouthed bait;
4) cosmetic material.
Along with updating of cultural technique, under traditional aquaculture model, pond dosage is large, the specification of breed variety is little and have a drug residue, by the application of chlorella, can well improve cultural method, reduce the use of medicine, form a kind of green ecological aquaculture model, reduce aquaculture cost, improve output, increase raiser's income.
The Main Function of chlorella in cultivation:
1) provide bait effect
Chlorella contains rich in protein can reach 50% left and right with dry weight basis, produce the good algae of protein and contain VITAMIN, biological polyoses, unsaturated fatty acids, chlorophyll, carotenoid, is shrimp seedling, juvenile crab shellfish, the best natural bait of fry, and natural immunity material and growth-promoting substance in chlorella protein, be that mixed feed is beyond one's reach.
2) water transfer effect
Along with the continuous rising of water temperature, water body easily breaks out blue-green algae under acidic conditions, brings harm easily to shrimp crab.In breeding process, use chlorella, it is played a leading role in water body, can effectively suppress the generation of blue-green algae, reduce to cultivate risk.
3) detoxification
Chlorella is to increasing water body dissolved oxygen, ammonia nitrogen, H in degradation water
2the toxin such as S, nitrite, has special efficacy.
Under the environment of heavy metal contamination, algae can be induced to produce metal binding protein and the metallothionein(MT) MT of a kind of plant chelating, and these two kinds of albumen detoxify in conjunction with poisonous metal ion by polypeptide.
4) increase dissolved oxygen in water
The photosynthesis of chlorella is the decades of times of other plant, and the strength of absorbing sun power is outstanding, is described as " the filling sun ", is one of biology that known photosynthesis is the strongest.So chlorella has important effect for the dissolved oxygen in pond.
Based on the extensive above-mentioned vital role of chlorella, demand to chlorella on market is larger, therefore need chlorella to carry out concentrating and separating, make live body chlorella cream, easy to use, as breeding bait, can reduce expenses, reduce risk, avoid raiser because of the impact of climate or other factors, algae water-mass density is rare or cultivate and unsuccessfully cause entire cost and waste of time.But the collection of micro-algae and purifying have restricted micro-algae and have cultivated extensive amplification and industrial applications.The collection method of current micro-algae mainly comprises: magnetic resolution, flocculation, air supporting etc.
Chinese patent CN102277300A discloses a kind of magnetic separation method of chlorella, and described method comprises: make Fe
3o
4nanoparticulate dispersed forms stable suspension; In chlorella nutrient solution, add described suspension, after stirring, through magnetic, separate the polymkeric substance that obtains chlorella and magnetic medium; Described polymkeric substance, after washing, adds hydrochloric acid reaction, finishes rear micro-filtration, washing and collects filtrate.
Chinese patent CN101095459A discloses a kind of method of utilizing the concentrated marine microalgae of flocculence, it is to the aqueous solutions of organic acids that adds chitosan in micro algae culturing liquid, the concentration that makes chitosan in micro algae culturing liquid is 0.3 ~ 8.0mg/L, then the pH value that regulates micro algae culturing liquid is 4.5 ~ 8.5, make micro-algae flocculation, and adopt static layering or air-float method to collect throw out.
While adopting alum to do the concentrated chlorella of flocculation agent, the jaundice of chlorella liquid color, the chlorella bad dispersibility of precipitation, cytopathy, illustrates that alum is larger to the toxic action of chlorella, and has reduced the nutritive value of chlorella.Generally, adopt flucculation process separate and collect micro-algae and need to use various magnetic resolution media, and magnetic resolution medium and micro-algae separation difficulty easily cause the pollution of target product.
Dissolved air flotation needs the auxiliary of magnetic resolution medium or tensio-active agent conventionally while applying in micro-algae, therefore exist and the same problem of flucculation process.
Summary of the invention
The invention provides a kind of preparation method of chlorella cream, the thickening efficiency that overcomes existing chlorella concentration method existence is low, easily causes chlorella to pollute, and destroys the shortcomings such as chlorella nutritive substance.
The present invention adopts following technical scheme:
A kind of preparation method of chlorella cream comprises the following steps:
(1) filter
The chlorella nutrient solution that is 50 ~ 1200mg/L by concentration carries out filtering separation, removes coarse particles, impurity in nutrient solution;
(2) concentrated
Nutrient solution after filtering is carried out to membrane sepn and obtain water-dialyzing and concentrated solution, water-dialyzing is back in the cultivation pool of chlorella.
(3) centrifugal
By the concentrated solution of step (2) gained, carry out centrifugation, make chlorella cream and water.
Described in step (1), be filtered into the one of screen filtration or fine fack; The screen cloth of described screen filtration is 50 ~ 100 orders.
The one of the described simmer down to micro-filtration of step (2) or ultrafiltration; The membrane module of described micro-filtration is the one of flat board, tubular type or rolling, and aperture is the microfiltration membrane of 0.1~0.2 micron; The mould material of described ultrafiltration is the one of ceramic membrane, metallic membrane or organic membrane; Membrane module is the one of board-like, tubular type, pillar.The molecular weight cut-off of ultra-filtration membrane is 1000~50000MWCO, and working conditions is: normal temperature~45 ℃, and the aperture of ceramic membrane is 50 ~ 200nm, the operating pressure of plate-type hyperfiltration, tubular type ultrafiltration and pillar ultrafiltration is 3~300kPa.
The described centrifugal rotating speed of step (3) is 2000~11000 revs/min, and optimum revolution is 4000~7000 revs/min.
From the above-mentioned description to structure of the present invention, compared to the prior art, tool of the present invention has the following advantages:
The present invention is particularly suitable for the preparation method of chlorella cream, by the combination of filtration, micro-filtration/ultrafiltration, centrifugal method, uses, and prepared chlorella cream purity is high, thickening efficiency is high, does not have flocculation agent residue problem, steady quality, chlorella content is high, and the shelf time is long.
Accompanying drawing explanation
Fig. 1 is preparation flow figure of the present invention.
Embodiment
1 explanation the specific embodiment of the present invention with reference to the accompanying drawings.
Embodiment 1
(1) filter
The chlorella nutrient solution that is 50mg/L by concentration carries out filtering separation, removes coarse particles, impurity in nutrient solution; The described screen filtration that is filtered into, screen cloth used is 75 orders.
(2) concentrated
Nutrient solution after filtering is carried out to membrane sepn and obtain water-dialyzing and concentrated solution, water-dialyzing is back in the cultivation pool of chlorella, and the concentration of gained chlorella concentrated solution is 10g/L; Described simmer down to ultrafiltration and concentration; The mould material of described ultrafiltration is ceramic membrane, and the aperture of ceramic membrane is 200nm.
(3) centrifugal
By the concentrated solution of step (2) gained, carry out centrifugation, make water ratio and be 80% chlorella cream and water; Described centrifugal rotating speed is 2000 revs/min.
Embodiment 2
(1) filter
The chlorella nutrient solution that is 600mg/L by concentration carries out filtering separation, removes coarse particles, impurity in nutrient solution; Described filtration unit adopts fine fack.
(2) concentrated
Nutrient solution after filtering is carried out to membrane sepn and obtain water-dialyzing and concentrated solution, water-dialyzing is back in the cultivation pool of chlorella, and the concentration of gained chlorella concentrated solution is 48g/L; Described concentrating unit is micro-filtration; The membrane module of described micro-filtration is flat sheet membrane, and aperture is 0.1 micron.
(3) centrifugal
By the concentrated solution of step (3) gained, carry out centrifugation, make water ratio and be 60% chlorella cream and water; Described centrifugal rotating speed is 11000 revs/min.
Embodiment 3
(1) filter
The chlorella nutrient solution that is 1200mg/L by concentration carries out filtering separation, removes coarse particles, impurity in nutrient solution; Described filtration unit adopts fine fack.
(2) concentrated
Nutrient solution after filtering is carried out to membrane sepn and obtain water-dialyzing and concentrated solution, water-dialyzing is back in the cultivation pool of chlorella, and the concentration of gained chlorella concentrated solution is 60g/L; Described simmer down to ultrafiltration and concentration; The mould material of described ultrafiltration is ceramic membrane, and the aperture of ceramic membrane is 50nm.
(3) centrifugal
By the concentrated solution of step (3) gained, carry out centrifugation, make water ratio and be 70% chlorella cream and water; Described centrifugal rotating speed is 6000 revs/min.
Above are only the specific embodiment of the present invention, but design concept of the present invention is not limited to this, allly utilizes this design to carry out the change of unsubstantiality to the present invention, all should belong to the behavior of invading protection domain of the present invention.
Claims (8)
1. a preparation method for chlorella cream, it comprises the following steps:
(1) filter
The chlorella nutrient solution that is 50 ~ 1200mg/L by concentration carries out filtering separation, removes coarse particles, impurity in nutrient solution;
(2) concentrated
Nutrient solution after filtering is carried out to membrane sepn and obtain water-dialyzing and concentrated solution, water-dialyzing is back in the cultivation pool of chlorella;
(3) centrifugal
By the concentrated solution of step (2) gained, carry out centrifugation, make chlorella cream and water.
2. the preparation method of a kind of chlorella cream as claimed in claim 1, is characterized in that: described in step (1), be filtered into the one that screen filtration or fine fack filter.
3. the preparation method of a kind of chlorella cream as claimed in claim 2, is characterized in that: the screen cloth of the described screen filtration of step (1) is 50 ~ 100 orders.
4. the preparation method of a kind of chlorella cream as claimed in claim 1, is characterized in that: the one of the described simmer down to micro-filtration of step (2) or ultrafiltration.
5. the preparation method of a kind of chlorella cream as claimed in claim 4, is characterized in that: the membrane module of the described micro-filtration of step (2) is the one of flat board, tubular type or rolling, and aperture is 0.1~0.2 micron.
6. the preparation method of a kind of chlorella cream as claimed in claim 4, it is characterized in that: the mould material of the described ultrafiltration of step (2) is the one of ceramic membrane, metallic membrane or organic membrane, membrane module is the one of board-like, tubular type, pillar, the molecular weight cut-off of ultra-filtration membrane is 1000~50000MWCO, working conditions is: normal temperature~45 ℃, the operating pressure of plate-type hyperfiltration, tubular type ultrafiltration and pillar ultrafiltration is 3~300kPa, and the aperture of ceramic membrane is 50~200nm.
7. the preparation method of a kind of chlorella cream as claimed in claim 1, is characterized in that: the described centrifugal rotating speed of step (3) is 2000~11000 revs/min.
8. the preparation method of a kind of chlorella cream as claimed in claim 7, is characterized in that: the described centrifugal optimum revolution of step (3) is 4000~7000 revs/min.
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Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
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CN104480018A (en) * | 2014-12-11 | 2015-04-01 | 临沂大学 | Method for preparing and storing fruiting dunaliella algae paste |
CN108504580A (en) * | 2018-04-19 | 2018-09-07 | 甘肃德福生物科技有限公司 | A kind of preparation method of the fresh and alive chlorella suspension improved the immunity of the human body |
CN109837203A (en) * | 2017-11-28 | 2019-06-04 | 浙江太阳石水处理有限公司 | A kind of efficient microalgae concentration technology based on ceramic membrane technology |
Citations (1)
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CN103468399A (en) * | 2013-09-24 | 2013-12-25 | 北京化工大学 | Method for extracting microbial oil |
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Publication number | Priority date | Publication date | Assignee | Title |
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CN103468399A (en) * | 2013-09-24 | 2013-12-25 | 北京化工大学 | Method for extracting microbial oil |
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宫庆礼等: "超滤技术在单胞藻浓缩中的应用", 《实验与技术》 * |
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Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104480018A (en) * | 2014-12-11 | 2015-04-01 | 临沂大学 | Method for preparing and storing fruiting dunaliella algae paste |
CN109837203A (en) * | 2017-11-28 | 2019-06-04 | 浙江太阳石水处理有限公司 | A kind of efficient microalgae concentration technology based on ceramic membrane technology |
CN108504580A (en) * | 2018-04-19 | 2018-09-07 | 甘肃德福生物科技有限公司 | A kind of preparation method of the fresh and alive chlorella suspension improved the immunity of the human body |
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Application publication date: 20140423 |