CN103733862A - Cultivation method for thuja seeds - Google Patents
Cultivation method for thuja seeds Download PDFInfo
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- CN103733862A CN103733862A CN201410014862.XA CN201410014862A CN103733862A CN 103733862 A CN103733862 A CN 103733862A CN 201410014862 A CN201410014862 A CN 201410014862A CN 103733862 A CN103733862 A CN 103733862A
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Abstract
The invention discloses a cultivation method for thuja seeds. The cultivation method includes the following steps: a, in the mid-to-late October every year, timely collecting the thuja seeds, picking cones during seed collection, putting the cones in indoor space for natural airing, screening out the seeds, putting the seeds into a ventilated cloth bag to be stored at the normal temperature for later use, and completing sowing before March of the next year; b, picking the thuja seeds, sterilizing the seeds by a HgCl2 (mercury chloride) solution with mass fraction of 1-2% for 5-10 minutes, soaking the seeds in a rooting powder solution with mass fraction of 0.001-0.002% for 8-12 hours, putting the seeds in a cultivation box, and cultivating the seeds at the temperature of 20-25 DEG C in a humid environment for 10-15 days to acquire budding seeds; C, sowing the budding seeds in media, placing the media under a sunshade, spraying mist and watering the seeds, performing routine management to acquire germinant seedlings, transplanting the seedlings to a seedbed with a sunshade after the seedlings grow to 3-5cm, and ensuring the seedlings to grow to acquire thuja seedlings. The soil of the seedbed is mixed soil formed by mixing of humus and river sand according to mass ratio of 1: 1.
Description
Technical field:
The invention belongs to field of plant growing technology, be specifically related to a kind of arborvitae seed propagation method.
Background technology:
Arborvitae Thuja sutchuenensis Franch. shrub or arbor; Branch is close, carries out, and the sprig of raw scale leaf is flat.Leaf squama shape, is born in the oblique side's shape of the leaf obovate of sprig central authorities, bossed longitudinal ridge, some longitudinal ridges have strip groove, long 1.5～3mm, wide 1.2～1.5mm, tip is blunt, and below is without gland point, the leaf ship shape of side, wide lanceolar, more central leaf is slightly short, wide 0.8-1mm, tip is blunt, curved in tip, two sides is green, without white powder.Male cone is closely oval, is about 2.5mm, and approximately 8 pairs, stamen intersects to life, connective width egg shape, and tip is blunt.Immature cone is about 5.5mm, ellipse, and 8 of fruit scales, intersect to life, the outmost fruit scale shape of falling ovum ellipse, there is a squamous tip below, top.
Be born in height above sea level 1400m Limestone Mountain, main product Chongqing City Chengkou County Daba Mountain.Also there is a small amount of distribution nature reserve, calyx mountain, Wanyuan City, Sichuan Province and Wudaoxia Nature Reserve, Baokang city, Hubei Province.The various soil zones of warm and moist are born in happiness, especially with well-grown on Limestone Mountain calcium soil.
Arborvitae is the most rare in the world gymnosperm.Once for the moment flouring in the Cretaceous period, have many types.When earth climatic environment generation transition, a large amount of extinct plants and animals become extinct in succession, but arborvitae relies on indomitable vitality to survive, and just population degenerates seriously, becomes in the world very rare " living fossil " species to today.
On April 9th, 1892, France's missionary's Bauer Joe Fargis (R.P.Farges) adopts at the Limestone Mountain at 1400 meters of of suitable small stream height above sea level of the southeast, Chengkou, Chongqing county the sample that obtains this Plants first, sample, by the collection of Paris National Museum of Natural History (mark this shop: Farges1158), becomes the type specimen of novel species subsequently.This is known to arborvitae science record the earliest in worldwide.But after this more than 100 year, scientist repeatedly went to the place of production to find, and arborvitae but disappears seemingly without foundation.1998 World Conservation Union (IUCN) classify arborvitae as the China of having died out peculiar rare plant.But through the travel of difficult searching, within 1999, arborvitae is rediscovered by Chinese Scientists, and develop and to the whole world, announce not extinction of arborvitae by the chairman A Liusi of world gymnosperm association.To this once " disappearance " seeds of more than 100 year, only have the research of a small amount of COMMUNITY CHARACTERISTICS and genetic diversity aspect.
Summary of the invention:
The object of the invention is to break through arborvitae seminal propagation obstacle, explore its seed germination mechanism, and provide a kind of easy and simple to handle, with low cost, the arborvitae seed propagation method of good seed can be provided for arborvitae industrialized development.
Arborvitae seed propagation method of the present invention, is characterized in that, comprises the following steps:
A, collect seed: in annual mid or late October, the arborvitae seed of immediately gathering, during seed collecting adopts cone and transfer Indoor Natural and dry in good time, sift out normal temperature in the cloth bag that seed packs ventilation into and preserve, standby, treat to complete sowing before March in next year;
B, presprouting of seeds: get arborvitae seed, first through mass fraction 1～2 ‰ HgCl
2solution disinfection 5-10 minute, then puts into seed mass fraction 0.001-0.002% rooting powder solution and soaks 8-12h, then be positioned in incubator, under moist environment, cultivation temperature is controlled at 20 ℃～25 ℃, cultivates 10～15 days, seed after being sprouted, the rate of sprouting 40%-50%;
C, sowing and seedling replanting: by the planting seed after sprouting in matrix, be placed under shading shed, spraying is watered, and Routine Management obtains the seedling germinateing, when seedling grows to 3～5cm, be transplanted on the seedbed with shading shed, the soil on seedbed is humus and the sandy composite soil mixing according to mass ratio 1:1, growth of seedling, obtain arborvitae seedling, described matrix is by humus soil and the river sand mixed composite soil of 1:1 or humus or river sand or vermiculite in mass ratio.
Preferably, before the March in next year described in step a, completed sowing.This time period is extremely important, because arborvitae seed is along with the prolongation of holding time, germination rate declines rapidly.Described in step c by the planting seed after sprouting in matrix, be placed under shading shed, the shading rate of the shading screen on shading shed is 70%, and being transplanted to described in step c has on the seedbed of shading shed, and the shading rate of the shading screen on shading shed is 60～70%.
The present invention has determined the best period of arborvitae seeds and sowing, has selected best seeding method and best sowing media, thereby has realized arborvitae seminal propagation.Arborvitae seed propagation method of the present invention has solved a difficult problem for arborvitae seed natural propagation rate extremely low (approximately 0.1%), by suitable processing, can larger raising arborvitae reproduction speed and emergence rate, not only can meet in production practices and the part demand of research work to seedling, also can solve the problem that vegetative propagation seedling resistance is poor, survival rate is low.The seedling stalwartness of seminal propagation, strong stress resistance, easily domestication, success rate is high, has good ecological benefits, economic benefit and social benefit.The method key technology difficult point is to find suitable time, the vernalization measure of arborvitae sowing, and technical operation is easy, with low cost, can provide good seed for arborvitae industrialized development.
Embodiment:
Following examples are to further illustrate of the present invention, rather than limitation of the present invention.
Embodiment 1:
A, seed collecting in good time: in China Chongqing province Chengkou County, annual mid or late October, the arborvitae Thuja sutchuenensis Franch seed of immediately gathering, during seed collecting adopts cone and transfers Indoor Natural and dry, sift out seed and pack normal temperature in the cloth bag of ventilation into and preserve, standby.
B, in March in next year, sow, adopt quartering to divide and get seed: be on experimental bench, to put three blank sheet of paper, centre is a 8K paper, both sides are 16K paper, the arborvitae seed sample of the 500g left and right in seed packet is poured on 8K paper, and stand rectangularity or square, require thickness even, then with ruler, along diagonal, arborvitae seed is divided into four triangles, then from every part, gets at random 1000 as sample.
C, presprouting of seeds: by take out above-mentioned 4 parts of arborvitae seed samples through mass fraction 1 ‰ HgCl
2solution disinfection 10 minutes, then the polymer solution in water of seed being put into mass fraction 0.001% root-inducing powder is soaked 12h, afterwards seed is placed in respectively in 4 culture dishes that are covered with 2 metafiltration paper, watering, be positioned over afterwards in incubator, cultivation temperature is controlled at 20 ℃～25 ℃ again, cultivates 10 days, seed after being sprouted, the rate 40～50% of sprouting.
D, sowing: the seed after sprouting is sowed respectively in 4 kinds of different matrix: a, fill humus soil and river sand (mass ratio 1:1); B, full humus; C, full river sand; D, full vermiculite, be contained in respectively high 50cm by matrix, and in the round plastic basin of diameter 40cm, the height of matrix is 40cm.100 seeds are broadcast in each processing, establish 3 repetitions.Plastic basin is put to the shading shed of 70% shading rate, Routine Management, every day, spraying was watered, about 30 days, humus and the river sand composite soil survival rate 20-25% of emerging, the humus soil survival rate 17-20% of emerging, the sandy survival rate 15-18% of emerging, the vermiculite soil survival rate 13-15% of emerging.
E, seedling replanting and management: when the seedling having germinateed grows to 3～5cm, be transplanted on seedbed, nursery soil is humus and sandy composite soil (mass ratio 1:1), builds frame on seedbed, adds a cover the shading screen of shade rate 60% on frame; After 7～10 days, seedling is taken root growth, within 2～3 days, waters once; About 150 days, seedling grows to 10～15cm, carefully pack, and conventional nursery bed management, obtains arborvitae seedling.
Embodiment 2:
A, collect seed: in China Chongqing province Chengkou County, annual mid or late October, the seed of immediately gathering, during seed collecting adopts cone and transfer Indoor Natural and dry in good time, sift out normal temperature in the cloth bag that seed packs ventilation into and preserve, standby.
B, in March in next year, sow, adopt quartering to divide and get seed: be on experimental bench, to put three blank sheet of paper, centre is a 8K paper, both sides are 16K paper, the arborvitae seed sample of the 500g left and right in seed packet is poured on 8K paper, and stand rectangularity or square, require thickness even, then with ruler, along diagonal, arborvitae seed is divided into four triangles, then from every part, gets at random 1000 as sample.
C, presprouting of seeds: by take out above-mentioned 4 parts of arborvitae seed samples through mass fraction 2 ‰ HgCl
2solution disinfection 5 minutes, then the polymer solution in water of seed being put into mass fraction 0.002% root-inducing powder is soaked 8h, afterwards seed is placed in respectively in 4 culture dishes that are covered with 2 metafiltration paper, watering, be positioned over afterwards in incubator, cultivation temperature is controlled at 20 ℃～25 ℃ again, cultivates 15 days, seed after being sprouted, the rate 40～50% of sprouting.
D, sowing: the seed after sprouting is sowed respectively in 4 kinds of different matrix: a, fill humus soil and river sand (mass ratio 1:1); B, full humus; C, full river sand; D, full vermiculite, be contained in respectively high 50cm by matrix, and in the round plastic basin of diameter 40cm, the height of matrix is 40cm.100 seeds are broadcast in each processing, establish 3 repetitions.Plastic basin is put to the shading shed of 70% shading rate, Routine Management, every day, spraying was watered, about 30 days, humus and the river sand composite soil survival rate 20-25% of emerging, the humus soil survival rate 17-20% of emerging, the sandy survival rate 15-18% of emerging, the vermiculite soil survival rate 13-15% of emerging.
E, seedling replanting and management: when the seedling having germinateed grows to 3～5cm, be transplanted on seedbed, soil is humus and sandy composite soil (mass ratio 1:1), builds frame on seedbed, adds a cover the shading screen of shade rate 70% on frame; After 7～10 days, seedling is taken root growth, within 2～3 days, waters once; About 150 days, seedling grows to 10～15cm, carefully pack, and conventional nursery bed management, obtains arborvitae seedling.
Claims (2)
1. an arborvitae seed propagation method, is characterized in that, comprises the following steps:
A, collect seed: in annual mid or late October, the arborvitae seed of immediately gathering, during seed collecting adopts cone and transfer Indoor Natural and dry in good time, sift out normal temperature in the cloth bag that seed packs ventilation into and preserve, standby, treat to complete sowing before March in next year;
B, presprouting of seeds: get arborvitae seed, first through mass fraction 1～2 ‰ HgCl
2solution disinfection 5-10 minute, then puts into seed mass fraction 0.001-0.002% rooting powder solution and soaks 8-12h, then be positioned in incubator, and under moist environment, cultivation temperature is controlled at 20 ℃～25 ℃, cultivates the seed after being sprouted 10～15 days;
C, sowing and seedling replanting: by the planting seed after sprouting in matrix, be placed under shading shed, spraying is watered, and Routine Management obtains the seedling germinateing, when seedling grows to 3～5cm, be transplanted on the seedbed with shading shed, the soil on seedbed is humus and the sandy composite soil mixing according to mass ratio 1:1, growth of seedling, obtain arborvitae seedling, described matrix is by humus soil and the river sand mixed composite soil of 1:1 or humus or river sand or vermiculite in mass ratio.
2. arborvitae seed propagation method according to claim 1, is characterized in that, before the March in next year described in step a, completes sowing.This time period is extremely important, because arborvitae seed is along with the prolongation of holding time, germination rate declines rapidly.Described in step c by the planting seed after sprouting in matrix, be placed under shading shed, the shading rate of the shading screen on its shading shed is 70%, and being transplanted to described in step c has on the seedbed of shading shed, and the shading rate of the shading screen on shading shed is 60～70%.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104871981A (en) * | 2015-07-01 | 2015-09-02 | 临沂大学 | Western red cedar tissue culture rapid propagation method |
| CN107360755A (en) * | 2017-08-09 | 2017-11-21 | 合肥沁牧生态农业有限公司 | A kind of arborvitae method for treating seeds for improving germination percentage |
Citations (4)
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|---|---|---|---|---|
| US4353184A (en) * | 1981-05-18 | 1982-10-12 | Weyerhaeuser Company | Method for asexual reproduction of coniferous trees |
| CN101699946A (en) * | 2009-09-30 | 2010-05-05 | 甘肃省祁连山水源涵养林研究院 | Pregermination method of Sabina Przewalskii kom seeds |
| CN101869027A (en) * | 2009-04-23 | 2010-10-27 | 上海上房园林植物研究所 | Cutting propagation method for orange Thuja |
| CN102960249A (en) * | 2012-11-30 | 2013-03-13 | 通化师范学院 | In-vitro efficient seedling cultivation method synchronous in rooting and growing by utilizing tender stem segments of thuja koraiensis |
-
2014
- 2014-01-13 CN CN201410014862.XA patent/CN103733862B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4353184A (en) * | 1981-05-18 | 1982-10-12 | Weyerhaeuser Company | Method for asexual reproduction of coniferous trees |
| CN101869027A (en) * | 2009-04-23 | 2010-10-27 | 上海上房园林植物研究所 | Cutting propagation method for orange Thuja |
| CN101699946A (en) * | 2009-09-30 | 2010-05-05 | 甘肃省祁连山水源涵养林研究院 | Pregermination method of Sabina Przewalskii kom seeds |
| CN102960249A (en) * | 2012-11-30 | 2013-03-13 | 通化师范学院 | In-vitro efficient seedling cultivation method synchronous in rooting and growing by utilizing tender stem segments of thuja koraiensis |
Non-Patent Citations (2)
| Title |
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| 侯伯鑫等: "《福建柏圃地育苗技术》", 《林业实用技术》 * |
| 朱莉等: "《崖柏和侧柏幼苗对自然降温的生理生化反应》", 《林业科学研究》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104871981A (en) * | 2015-07-01 | 2015-09-02 | 临沂大学 | Western red cedar tissue culture rapid propagation method |
| CN107360755A (en) * | 2017-08-09 | 2017-11-21 | 合肥沁牧生态农业有限公司 | A kind of arborvitae method for treating seeds for improving germination percentage |
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