CN103728452B - Colloidal gold immunoassay kit for detecting aflatoxin B1 and preparation method of kit - Google Patents
Colloidal gold immunoassay kit for detecting aflatoxin B1 and preparation method of kit Download PDFInfo
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- CN103728452B CN103728452B CN201410032159.1A CN201410032159A CN103728452B CN 103728452 B CN103728452 B CN 103728452B CN 201410032159 A CN201410032159 A CN 201410032159A CN 103728452 B CN103728452 B CN 103728452B
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- colloidal gold
- aflatoxin
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- OQIQSTLJSLGHID-WNWIJWBNSA-N aflatoxin B1 Chemical compound C=1([C@@H]2C=CO[C@@H]2OC=1C=C(C1=2)OC)C=2OC(=O)C2=C1CCC2=O OQIQSTLJSLGHID-WNWIJWBNSA-N 0.000 title claims abstract description 41
- 229930020125 aflatoxin-B1 Natural products 0.000 title claims abstract description 41
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 title claims abstract description 39
- 239000002115 aflatoxin B1 Substances 0.000 title claims abstract description 37
- 238000003018 immunoassay Methods 0.000 title claims abstract description 11
- 238000002360 preparation method Methods 0.000 title claims description 11
- 238000001514 detection method Methods 0.000 claims abstract description 38
- 150000007523 nucleic acids Chemical class 0.000 claims abstract description 27
- 108020004707 nucleic acids Proteins 0.000 claims abstract description 26
- 102000039446 nucleic acids Human genes 0.000 claims abstract description 26
- 239000000020 Nitrocellulose Substances 0.000 claims abstract description 25
- 229920001220 nitrocellulos Polymers 0.000 claims abstract description 25
- 238000012360 testing method Methods 0.000 claims abstract description 25
- 239000000243 solution Substances 0.000 claims description 33
- 241001494479 Pecora Species 0.000 claims description 15
- 230000000274 adsorptive effect Effects 0.000 claims description 13
- 238000000034 method Methods 0.000 claims description 12
- 239000010931 gold Substances 0.000 claims description 7
- 229910052737 gold Inorganic materials 0.000 claims description 7
- 238000013016 damping Methods 0.000 claims description 6
- 239000012530 fluid Substances 0.000 claims description 6
- 239000002250 absorbent Substances 0.000 claims description 4
- 230000002745 absorbent Effects 0.000 claims description 4
- 239000000084 colloidal system Substances 0.000 claims description 4
- 239000002245 particle Substances 0.000 claims description 4
- 238000001556 precipitation Methods 0.000 claims description 4
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 claims description 3
- 239000007853 buffer solution Substances 0.000 claims description 3
- 239000003365 glass fiber Substances 0.000 claims description 3
- 239000000463 material Substances 0.000 claims description 3
- 239000002773 nucleotide Substances 0.000 claims description 3
- 125000003729 nucleotide group Chemical group 0.000 claims description 3
- 239000012528 membrane Substances 0.000 abstract description 10
- 230000035945 sensitivity Effects 0.000 abstract description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract description 3
- 238000001179 sorption measurement Methods 0.000 abstract description 2
- 238000010521 absorption reaction Methods 0.000 abstract 2
- 238000003908 quality control method Methods 0.000 abstract 2
- 241000283707 Capra Species 0.000 abstract 1
- 108091028043 Nucleic acid sequence Proteins 0.000 abstract 1
- 241000209094 Oryza Species 0.000 description 7
- 235000007164 Oryza sativa Nutrition 0.000 description 7
- 235000009566 rice Nutrition 0.000 description 7
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 6
- 239000003153 chemical reaction reagent Substances 0.000 description 5
- 238000004587 chromatography analysis Methods 0.000 description 5
- 101100449517 Arabidopsis thaliana GRH1 gene Proteins 0.000 description 4
- 101100434479 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) AFB1 gene Proteins 0.000 description 4
- 239000006228 supernatant Substances 0.000 description 4
- 235000013339 cereals Nutrition 0.000 description 3
- 238000002372 labelling Methods 0.000 description 3
- 239000000427 antigen Substances 0.000 description 2
- 102000036639 antigens Human genes 0.000 description 2
- 108091007433 antigens Proteins 0.000 description 2
- 238000003556 assay Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 239000000284 extract Substances 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 239000000758 substrate Substances 0.000 description 2
- 241000282414 Homo sapiens Species 0.000 description 1
- 229920002538 Polyethylene Glycol 20000 Polymers 0.000 description 1
- 238000013019 agitation Methods 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000011068 loading method Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000010339 medical test Methods 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 230000003252 repetitive effect Effects 0.000 description 1
- 230000000630 rising effect Effects 0.000 description 1
- 230000009870 specific binding Effects 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/577—Immunoassay; Biospecific binding assay; Materials therefor involving monoclonal antibodies binding reaction mechanisms characterised by the use of monoclonal antibodies; monoclonal antibodies per se are classified with their corresponding antigens
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
Abstract
The invention discloses a colloidal gold immunoassay kit for detecting aflatoxin B1. The colloidal gold immunoassay kit comprises a piece of detection test paper, wherein the detection test paper comprises a soleplate, and a sample pad, a combination pad, a nitrocellulose membrane and a water absorption pad, which are adhered to the soleplate and sequentially in lap joint with one another; one side, close to the combination pad, of the nitrocellulose membrane is provided with a detection line, and one side, close to the water absorption pad, of the nitrocellulose membrane is provided with a quality control line; the combination pad is coated with colloidal gold which is wrapped by aflatoxin B1 monoclonal antibody and single-stranded nucleic acid; the detection line is coated with BSA-AFB1; the quality control line is coated with goat anti mouse IgG; the single-stranded nucleic acid sequence is as shown in SEQ ID NO.1. The kit has the characteristics that while the antibody is marked, a section of single-stranded nucleic acid is cooperatively marked on the surface of the colloidal gold, so that the non-specificity adsorption can be effectively reduced, the consumption of the marked antibody can be reduced, and the sensitivity of the prepared immunoassay kit reaches 0.5ng/mL.
Description
Technical field
The present invention relates to a kind of medical testing product and preparation method thereof, particularly relate to a kind of colloidal gold immunoassay kit detecting aflatoxin B1 and preparation method thereof.
Background technology
Immunochromatographic method is a kind of quick diagnosis technology based on immune colloidal solid of rising nineteen nineties, its principle is a certain zone special antibody being first fixed on nitrocellulose membrane, when the nitrocellulose filter end thereof contacts of this drying is after sample, due to capillarity, sample will move forward along this film, when moving to the region being fixed with antibody, in sample corresponding antigen namely with this antibody generation specific binding, thus realize specific immunodiagnosis.
The biomacromolecule of the specific recognition detection thing that conventional immunochromatographic method is used is all antibody, reacted by antigen-antibody specific recognition, the immune colloidal solid be marked on antigen or antibody makes detection line and nature controlling line show certain color, thus realizes specific immunodiagnosis.But the method detects not fast with sensitive, and also needs to use corresponding instrument and equipment with antibody test, operates not easy.
Summary of the invention
The present invention is intended to overcome the deficiencies in the prior art, providing a kind of and makes colloidal gold immunochromatographiassay assay reagent box, by showing redness on detection line and nature controlling line, realizing direct-detection aflatoxin B1 in the sample to which.
For achieving the above object, technical scheme provided by the invention is:
The colloidal gold immunoassay kit of described detection aflatoxin B1 comprises Test paper; Described Test paper comprises base plate, to be bonded on base plate and the sample pad overlapped successively, pad, nitrocellulose filter and adsorptive pads; Side near pad on described nitrocellulose membrane is provided with detection line, and the side near adsorptive pads on nitrocellulose membrane is provided with nature controlling line; Described pad scribbles the collaurum by mouse-anti aflatoxin B1 monoclonal antibody and single-chain nucleic acid bag quilt; Described detection line scribbles BSA-AFB1; Described nature controlling line scribbles sheep anti-mouse igg; Described single strand nucleotide sequence is as shown in SEQ ID NO.1.
Preferably, described base plate is PVC board; The material of sample pad and pad is glass fibre; Described adsorptive pads is absorbent filter.Described Test paper is packed by rigid plastic card.
Present invention also offers the method for mentioned reagent box, comprise the steps:
(1) with mouse-anti aflatoxin B1 monoclonal antibody and the single-chain nucleic acid bag as shown in SEQ ID NO.1 by collaurum, must wrap by after colloidal gold solution;
(2) by bag by after colloidal gold solution be sprayed on pad, dry for standby;
(3) detection line position specking BSA-AFB1 solution on nitrocellulose membrane; At nature controlling line position specking sheep anti-mouse igg solution, dry for standby;
(4) sample pad, pad, nitrocellulose filter and adsorptive pads overlapped successively and be bonded on base plate, being cut into test strip, test strip rigid plastic card is packed.
Preferably, the described collaurum diameter of step (1) is 13-40nm.Step (1) described mouse-anti aflatoxin B1 monoclonal antibody and the single-chain nucleic acid bag as shown in SEQ ID NO.1 are referred to by collaurum and first mouse-anti aflatoxin B1 monoclonal antibody are mixed with the such as single-chain nucleic acid shown in SEQ ID NO.1, obtain antibody nucleic acids potpourri, then antibody nucleic acids potpourri is added in colloidal gold solution, now, in colloidal gold solution, colloid gold particle concentration is 3.5-4.5nmol/L, preferred 4nmol/L, mouse-anti aflatoxin B1 MAb concentration is 4.5-5.5 μ g/mL, preferably 5 μ g/mL, single-chain nucleic acid concentration is 8-12 μ g/mL, preferably 10 μ g/mL, again by centrifugal for the colloidal gold solution adding antibody nucleic acids potpourri, precipitation is dissolved in Tris-HCL buffer solution, must wrap by after colloidal gold solution, for subsequent use.
Step (3) described BSA-AFB1 solution preparation method is that 2-4mg/mLBSA-AFB1 is dissolved in PBS damping fluid that to obtain BSA-AFB1 concentration be 0.7mg/mLBSA-AFB1 solution by concentration; In described sheep anti-mouse igg solution, the concentration of sheep anti-mouse igg is 0.8-1.2mg/mL, preferred 1mg/mL.
When using this kit, sample to be measured is joined loading wells, sample to be measured is under capillary action along sample pad, pad, nitrocellulose filter to absorbent filter extreme direction chromatography, during detection, if containing in sample to be measured detects thing aflatoxin B1, and concentration is higher than 0.5 nanograms/milliliter, the golden labeling antibody of aflatoxin B1 first on pad is combined and makes it to redissolve, and the potpourri after redissolution continues chromatography on nitrocellulose filter, no longer combine with the BSA-AFB1 on detection line, mark gold grain continues forward, when arriving nature controlling line region, combines with the sheep anti mouse Ig G of bag quilt on it, show the red lines of collaurum, this is nature controlling line, during detection, if not containing in sample to be measured detects thing aflatoxin B1, golden labeling antibody on pad redissolves, and the potpourri after redissolution continues chromatography on nitrocellulose filter, and the BSA-AFB1 on detection line combines, there is the redness of mark collaurum in such detection line position, mark gold grain continues forward, when arriving nature controlling line region, combines with the sheep anti mouse Ig G of bag quilt on it, show the red lines of collaurum, this is nature controlling line, during detection, if aflatoxin B1 concentration is lower than 0.5 nanograms/milliliter in determinand, the golden labeling antibody of aflatoxin B1 first on pad is combined and makes it to redissolve, potpourri after redissolution continues chromatography on nitrocellulose filter, combine with the BSA-AFB1 on detection line again, such detection line position also there will be the redness of mark collaurum, due to Immune competition reaction, aflatoxin B1 concentration ratio 0.5 nanograms/milliliter is lower, the redness of detection line is redder, mark gold grain continues forward, when arriving nature controlling line region, combine with the sheep anti mouse Ig G of bag quilt on it, show the red lines of collaurum, this is nature controlling line.
The feature of this kit is while labelled antibody, and be marked at gold colloid surface by collaborative for one section of single-chain nucleic acid, effectively reduce non-specific adsorption, reduce the consumption of labelled antibody, the immune chromatography reagent kit made, sensitivity reaches 0.5ng/mL.
Accompanying drawing explanation
Fig. 1 is Test paper structural representation in kit of the present invention;
Fig. 2 is principle schematic of the present invention;
Fig. 3 is testing process schematic diagram of the present invention; Without detecting thing, or detect substrate concentration lower than minimal detectable concentration, detection line is red (for dark in figure); There is detection thing: and detect substrate concentration higher than minimal detectable concentration, detection line position colourless (being blank in figure);
Fig. 4 is immunochromatographyassay assay result process decision chart of the present invention, and wherein, T represents detection line, and C represents nature controlling line.
In figure: 1, base plate; 2, sample pad; 3, pad; 4, nitrocellulose filter; 5, detection line; 6, nature controlling line; 7 adsorptive pads.
Embodiment
Embodiment 1
See Fig. 1, the colloidal gold immunoassay kit of described detection aflatoxin B1, comprises Test paper; Described Test paper comprises base plate 1, to be bonded on base plate 1 and the sample pad 2 overlapped successively, pad 3, nitrocellulose filter 4 and adsorptive pads 7; Side near pad 3 on described nitrocellulose membrane 4 is provided with detection line 5, and the side near adsorptive pads 7 on nitrocellulose membrane 4 is provided with nature controlling line 6; Described pad 3 scribbles the collaurum by mouse-anti aflatoxin B1 monoclonal antibody and single-chain nucleic acid bag quilt; Described detection line 5 scribbles BSA-AFB1; Described nature controlling line 6 scribbles sheep anti-mouse igg; Described single strand nucleotide sequence is 5 '-AAAAAAAAAAAAAAA-3 ' (SEQ ID NO.1).Described base plate 1 is PVC board; The material of sample pad 2 and pad 3 is glass fibre; Described adsorptive pads 7 is absorbent filter.Described Test paper is packed by rigid plastic card.
The actual use of kit
Introduce using method of the present invention below, can not as restriction of the present invention.
1. reagent and sample: AFB1 titer is purchased from SIGMA company, and rice is purchased from supermarket.
2. positive preparation: AFB1 titer is diluted to 1mg/mL with 0.0lM pH7.4PBS damping fluid.Rice is ground into powder, take 1 gram and put into centrifuge tube or beaker, add the 0.0lM pH7.4PBS damping fluid 10mL containing 30% (volume ratio) methyl alcohol, vortex mixing 5min, with small handheld hydro-extractor 4000 revs/min of centrifugal 5min, leave and take supernatant.Respectively to the AFB1 titer adding different volumes in 8 pipe rice extracts, make the positive that final concentration is 0ng/ml, 0.5ng/ml, 0.75ng/ml, 1ng/ml, 2ng/ml, 5ng/ml, 10ng/ml, 20ng/ml.
3. cloudy sample preparation: get 8 pipe rice extracts, do not add AFB1 titer, but add the PBS damping fluid containing 30% (percent by volume) methyl alcohol identical with titer volume added by each pipe positive.
4. from 16 pipe measuring samples, draw 100 μ L liquid respectively, be added drop-wise in the sample well of test card, after 10min, observe testing result.
5. testing result: repeat above experiment three times, negative sample testing result is all negative.Positive testing result is as shown in table 1:
Table 1: aflatoxin B1 detection kit sensitivity
4. can show that the sensitivity of aflatoxin B1 detection kit of the present invention is 1ng/ml from above-mentioned gradient experiment result.During due to sample preparation, 10 times of dilutions are carried out, can extrapolate that to detect in rice sample containing aflatoxin B1 sensitivity be 10ng/ml, must not more than the standard of 10g/kg about containing aflatoxin B1 in rice according to country, i.e. about 10ng/ml, this kit can as the qualitative detection reagent of Rapid identification rice containing aflatoxin B1.
Embodiment 2
Prepare the method for kit described in embodiment 1, comprise the steps:
(1) diameter is the centrifugal 15min of colloid gold particle of 13-40nm, and sucking-off supernatant, adds sterilized water, and repetitive operation several times, obtains colloidal gold solution; Be that the single-chain nucleic acid of 5 '-AAAAAAAAAAAAAAA-3 ' (SEQ ID NO.1) mixes by mouse-anti aflatoxin B1 monoclonal antibody and sequence, obtain antibody nucleic acids potpourri, then antibody nucleic acids potpourri is slowly added in colloidal gold solution under magnetic agitation 1000rpm/min condition, stir 45min, require that in colloidal gold solution now, colloid gold particle concentration is 4nmol/L, mouse-anti aflatoxin B1 MAb concentration is 5 μ g/mL, and single-chain nucleic acid concentration is 10 μ g/mL; Colloidal gold solution centrifugal 20min under 1000rpm/min, 4 DEG C of conditions of antibody nucleic acids potpourri will be added again, go precipitation, by supernatant centrifugal 20min under 11000rpm/min condition again, suck supernatant, precipitation is dissolved in the Tris-HCL buffer solution of the 0.02M pH8.0 containing 0.5% (mass percentage content) PEG 20000, obtain by mouse-anti aflatoxin B1 monoclonal antibody and single-chain nucleic acid bag by after colloidal gold solution, save backup in 4 DEG C;
(2) by bag by after colloidal gold solution be sprayed on pad, quantity for spray be 5 microlitres/centimetre, 37 DEG C dry 2h, 4 DEG C save backup;
(3) detection line position specking BSA-AFB1 solution on nitrocellulose membrane; At nature controlling line position specking sheep anti-mouse igg solution, dry 2h for 37 DEG C, good seal to be placed in room temperature for subsequent use; Described BSA-AFB1 solution preparation method is that 2-4mg/mLBSA-AFB1 is dissolved in PBS damping fluid that to obtain BSA-AFB1 concentration be 0.7mg/mLBSA-AFB1 solution by concentration; In described sheep anti-mouse igg solution, the concentration of sheep anti-mouse igg is 1mg/mL;
(4) sample pad, pad, nitrocellulose filter and adsorptive pads overlapped successively and be bonded on base plate, be cut into the test strip of 3-4 ㎜ width, test strip rigid plastic card is packed, the colloidal gold immunoassay kit of aflatoxin B1 must be detected.
Draw sample 0.1mL to be measured and instill well, if nature controlling line does not develop the color for invalid, if the aobvious red and nature controlling line of detection line does not develop the color and is considered as invalid yet, if redness all appears in nature controlling line and detection line, represent and detecting in sample the aflatoxin B1 had higher than minimal detectable concentration 0.5ng/mL.
SEQUENCE LISTING
<110> Hunan University
<120> colloidal gold immunoassay kit detecting aflatoxin B1 and preparation method thereof
<160> 1
<170> PatentIn version 3.3
<210> 1
<211> 15
<212> DNA
<213> homo sapiens
<400> 1
aaaaaaaaaa aaaaa
Claims (7)
1. detect a colloidal gold immunoassay kit for aflatoxin B1, comprise Test paper; Described Test paper comprises base plate (1), is bonded in base plate (1) and goes up and the sample pad (2) overlapped successively, pad (3), nitrocellulose filter (4) and adsorptive pads (7); The upper side near pad (3) of described nitrocellulose filter (4) is provided with detection line (5), and the upper side near adsorptive pads (7) of nitrocellulose filter (4) is provided with nature controlling line (6); It is characterized in that, described pad (3) scribbles the collaurum by mouse-anti aflatoxin B1 monoclonal antibody and single-chain nucleic acid bag quilt; (5) scribble BSA-AFB1 to described detection line; (6) scribble sheep anti-mouse igg to described nature controlling line; Described single strand nucleotide sequence is as shown in SEQ ID NO.1.
2. kit as claimed in claim 1, it is characterized in that, described base plate (1) is PVC board; The material of sample pad (2) and pad (3) is glass fibre; Described adsorptive pads (7) is absorbent filter.
3. kit as claimed in claim 1, it is characterized in that, described Test paper is packed by rigid plastic card.
4. prepare the method for kit described in any one of claims 1 to 3, comprise the steps:
(1) with mouse-anti aflatoxin B1 monoclonal antibody and the single-chain nucleic acid bag as shown in SEQ ID NO.1 by collaurum, must wrap by after colloidal gold solution;
(2) by bag by after colloidal gold solution be sprayed on pad, dry for standby;
(3) detection line position specking BSA-AFB1 solution on nitrocellulose filter; At nature controlling line position specking sheep anti-mouse igg solution, dry for standby;
(4) sample pad, pad, nitrocellulose filter and adsorptive pads overlapped successively and be bonded on base plate, being cut into test strip, test strip rigid plastic card is packed.
5. method as claimed in claim 4, it is characterized in that, step (1) collaurum diameter is 13-40nm.
6. method as claimed in claim 5, it is characterized in that, step (1) described mouse-anti aflatoxin B1 monoclonal antibody and the single-chain nucleic acid bag as shown in SEQ ID NO.1 are referred to by collaurum and first mouse-anti aflatoxin B1 monoclonal antibody are mixed with the such as single-chain nucleic acid shown in SEQ ID NO.1, obtain antibody nucleic acids potpourri, then antibody nucleic acids potpourri is added in colloidal gold solution, now, in colloidal gold solution, colloid gold particle concentration is 3.5-4.5nmol/L, mouse-anti aflatoxin B1 MAb concentration is 4.5-5.5 μ g/mL, single-chain nucleic acid concentration is 8-12 μ g/mL, again by centrifugal for the colloidal gold solution adding antibody nucleic acids potpourri, precipitation is dissolved in Tris-HCL buffer solution, must wrap by after colloidal gold solution, for subsequent use.
7. method as claimed in claim 6, is characterized in that, step (3) described BSA-AFB1 solution preparation method is that 2-4mg/mLBSA-AFB1 is dissolved in PBS damping fluid that to obtain BSA-AFB1 concentration be 0.7mg/mLBSA-AFB1 solution by concentration; In described sheep anti-mouse igg solution, the concentration of sheep anti-mouse igg is 0.8-1.2mg/mL.
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| CN109900905A (en) * | 2019-02-28 | 2019-06-18 | 中国科学院广州生物医药与健康研究院 | A kind of colloidal gold strip and preparation method detecting carcinomebryonic antigen |
| CN116819070B (en) * | 2023-07-10 | 2024-03-22 | 希莱乐检(郑州)生物科技有限公司 | Test strip, detection device and detection method for detecting target in body fluid |
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| CN103728452A (en) | 2014-04-16 |
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