CN103720773A - Degerming agent specially used for carp breeding - Google Patents

Abstract

The invention relates to a degerming agent specially used for carp breeding. The degerming agent is prepared via reasonable combination of a plurality of Chinese herbal medicine extracts and special processes. The degerming agent is suitable for the field of carp breeding, can be used for preventing leprnorthsis, leukoderma, stigmatosis, Intestinal bleeding, and tail-rot disease, and possesses obvious killing effects on pathogenic microbes such as vibrio, aeromonas, and mould. The compound degerming agent prepared from Chinese herbal medicines is characterized in that: no drug stimulation, toxic effect or side effect is caused; sterilization efficiency is high; and the degerming agent is green, and is friendly to the environment.

Description

The special-purpose bacteria remover of carp culture

technical fieldthe present invention relates to the special-purpose bacteria remover of a kind of carp culture, it is characterized in that extracting multiple Chinese herbal medicine is that main warp reasonable compatibility forms.The active ingredient of formula is iodate N2 N-trimethyl chitosan TMC, Folium Isatidis, Radix Isatidis, Folium Eucalypti Robustae, Herba Senecionis Scandentis, vitamin B ₁₂, vitamin B ₁₃process configuration forms.This product is applicable to carp culture field, and prevention scale protrusion disease, leukoderma, stigmatosis, enteritis hemorrhagic disease, tail-rot disease disease problem, for the sex pheromone that causes disease, as vibrio, Aeromonas, mycete are all measured obvious degermation.The present invention adopts take Chinese herbal medicine as main composite sterilizing agent, and its feature is to have Nantural non-toxic side effect, efficient sterilizing, without medicine irritation, and the multiple advantage of environmental protection.

background technologycyprinus carpio, a lot of through artificial culture's kind, as red Cyprinus carpio, group Cyprinus carpio, ctenopharyn odon idellus, lotus color Cyprinus carpio, Cyprinus carpio L., fiery Cyprinus carpio, Hibisci Mutabilis Cyprinus carpio, pocket Cyprinus carpio etc.Kind is different, and its figure varies in color, and is deeply subject to everybody liking.From calendar year 2001, along with the fast development of aquaculture, the innovation of carp culture technology, as one of important topic in pet fish aquaculture technology, has obtained the concern of each layer of society.The cultivation of Cyprinus carpio is various in style, its color and luster, and speckle and lines are as the important symbol of dividing height class, are extensively subject to pursuing of pet fish fan.Along with developing rapidly of economy and cultural technique, cultured area is also in continuous expansion, and disease problem is also following.Existing carp culture mode adopts pond separation cultivation method to carry out mostly, this method is convenient to the Cyprinus carpio classified cultivation of different cultivars, but its drawback is very easily to cause water area excessive, and illness outbreak is rapid, water body is processed operating difficulties, and raiser more needs to focus on the maintenance of daily water body.Carp culture has strict requirement to the control of water quality and bottom soil property, and inappropriate use medicine or operation all can cause malignant bacteria and infect, and raiser is caused to great loss, and this is also the major reason that has hindered the development of carp culture industry.Harm for antagonism disease, existing employing penicillin, the effect of the antibiotic broad-spectrum sterilization of oxytetracycline and fluoroquinolones in short time effect obviously, is only applicable to the first aid operation of the extensive epidemic situation of fulminant, and long-term or continual use easily causes the generation of drug resistance.Except antibiotic, halogen chemosterilant is as the conventional medicine of safeguarding, in carrying out preventing and controlling process, maintenance effects shows slightly inferior, and the strong oxidizers such as that halogen chemosterilant mostly is is chloride, iodine, bromine, there is corrosivity strong, safety coefficient is low, once drug dose is controlled the improper existence that will threaten Fish.Through fastidious, find, Chinese herbal medicine is as the quintessence of Chinese culture place of a kind of China Chinese medicine, there is the clinical application history of more than one thousand years to go through, there is abundant clinical application experience, treatment various diseases is all had to good effect, it is characterized in that thering is broad-spectrum sterilization, no side effects, without drug resistance reaction, can life-time service, the multiple advantage such as medicament sources is abundant.The present invention is through screening multiple Chinese herbal medicine, and rationally composite forming, according to fish species difference, researches and develops body constitution and the customized special-purpose bacteria remover of medicine adaptive capacity thereof for Cyprinus carpio, effectively prevents and treats various antibacterials, fungus-caused Cyprinus carpio disease problem.

summary of the inventionmain points of the present invention are to adopt multiple pure natural Chinese herbal medicine and biological preparation is composite forms, according to combination principle, through grinding, 60% ethanol water-bath warm macerating, filters, centrifugal, blend, the techniques such as stirring are made, and have greatly improved the pharmacological action of Chinese herbal medicine, finally make the special-purpose bacteria remover of a kind of carp culture.

The formula following (component and percentage by weight % thereof) that the present invention selects: iodate N2 N-trimethyl chitosan TMC 4.0-9.0; Folium Isatidis 3.5-8.0; Radix Isatidis 6.0-9.0; Folium Eucalypti Robustae 4.0-7.0; Herba Senecionis Scandentis 3.5-7.0; Vitamin B ₁₂2.0-5.0; Vitamin B ₁₃3.0-6.0; Water to 100.0.

The action principle of iodate N2 N-trimethyl chitosan TMC is because itself being cation macromolecular polymer; can with cell surface with negative charge protein, teichoic acid, lipopolysaccharide etc. there is flocculation; destroy the permeability barrier effect of cytoplasma membrane; cause the integrity of cell to destroy; nutrient substance runs off, and reaches antibacterial action; Or adsorb the antibacterial with negative charge, synthesizing of interference cell wall, makes cell wall lysis and death.

The action principle of Folium Isatidis and Radix Isatidis is: Folium Isatidis is the leaf of cruciferae isatis and acanthaceous plant Herba Kalimeridis, polygonum tinctorium ait., and Radix Isatidis is exactly the root of Isatis indigotica Fort..Its effective ingredient is Folium Isatidis element, indigo glycoside etc.The two all has wide antibacterial and antivirus action and antipyretic and antidote functions.The two has stronger synergistic corrosion virus effect.

The principle of drug action of Folium Eucalypti Robustae is to have dispelling wind antipyretic, inhibiting bacteria and diminishing inflammation, anticorrosion antipruritic effect; The gallic acid in vitro tests (flat band method) raising from cured leaf, to Mlcs such as staphylococcus aureus, sarcina, Neisseria gonorrhoeae, bacillus pyocyaneus, being 2.5mg/ml, is 5mg/ml to Mlcs such as streptococcus pneumoniae, escherichia coli, bacterium flexneri, Bacillus typhi, Salmonella paratyphi A.The extract of gallic acid and Folium eucalypti globueli (Eucalyptus globulus Labill.), in vitro infected by influenza has certain inhibitory action.Its decoct is all had to inhibitory action to bacillus such as staphylococcus aureus, Hemolytic streptococcus and dysentery, typhoid fever, large intestine, green pus in vitro.Robustaol second element has inhibitory action to staphylococcus aureus and bacillus subtilis in the test of pipe butterfly method, and minimum bacteriostasis concentration is 63g/ml.Large Folium eucalypti globueli (Eucalyptus globulus Labill.) decoct is to antibacterial performance antibacterial actions such as staphylococcus, dysentery bacteriums.

The principle of drug action of Herba Senecionis Scandentis is that its effective ingredient is flavone compound, and this material has the mechanism of action of anti-Staphylococcus aureus.Scanning electron microscopic observation is found after Herba Senecionis Scandentis infusion and the effect of Herba Senecionis Scandentis total flavones Contained Serum, there is significant change in the form of Staphylococcus aureus, and the metamorphosis of Staphylococcus aureus is not obvious after the effect of negative control group serum, illustrate that Contained Serum has certain destruction to the cell wall of test organisms and cell membrane, and once cell wall and cell membrane are destroyed, its bacterial cell just can not maintain the intrinsic form of cell, the inside and outside mass exchange of born of the same parents is obstructed, the various enzymes that are incorporated on after birth can not normally play a role, cause bacterial cell to reduce absorption of nutrient ingredients, biomacromolecule biosynthesis block.The antibacterial mechanisms of Herba Senecionis Scandentis is to show antibacterial and bactericidal action by suppressing the synthesizing of DNA, RNA, protein and Peptidoglycan of staphylococcus aureus, the effective ingredient of its effect is relevant with flavone compound, and this has antibacterial action composition consistent with flavone compound one class.

The action principle of vitamin B12: its manufacture and renew the erythrocyte in body, can prevent anemia, contribute to growing, the nervous system of keeping fit, subduction allergic conditions, promotes the equilibrant of health.

The action principle of orotic acid: it contributes to the metabolism of vitamin B, can together carry out metabolism with vitamin B12 and folic acid, very important to the recovery of cell and repairing.

The preparation method of product of the present invention is:

1) Folium Isatidis, Radix Isatidis, Folium Eucalypti Robustae, Herba Senecionis Scandentis are through grinding;

2) 60% alcoholic solution thermostat water bath warm macerating 1 ~ 2h, filters;

3) Chinese herbal medicine fluid leaching is mixed, 2000 revs/min are carried out centrifugally, extract the medicinal liquid of supernatant;

4) by iodate N2 N-trimethyl chitosan TMC, vitamin B ₁₂, vitamin B ₁₃be mixed in proportion be added to extract supernatant medicinal liquid in reactor, be uniformly mixed;

5) with sterile distilled water, be settled to desired concn, low temperature is sealed up for safekeeping standby.

The present invention has the following advantages:

(1) selected natural high-quality Chinese herbal medicine refines, nontoxic residue-free;

(2) Chinese herbal medicine antibacterial, sterilization that screens multiple epistasis carries out compatibility, has the effect of high-efficiency broad spectrum sterilization;

(3) nonirritant reaction, free from extraneous odour;

(4) can improve immune effect;

(5) the experiment proved that, active ingredient is stable, antimicrobial long-acting, long shelf-life.

the specific embodimentbelow in conjunction with embodiment, the present invention is described in further detail:

According to following table institute column data (percentage by weight %) and described step thereof, be configured:

Formula one:

Iodate N2 N-trimethyl chitosan TMC 4.0-9.0

Folium Isatidis 3.5-8.0

Radix Isatidis 6.0-9.0

Folium Eucalypti Robustae 4.0-7.0

Herba Senecionis Scandentis 3.5-7.0

Vitamin B ₁₂2.0-5.0

Vitamin B ₁₃3.0-6.0

Water to 100.0

Formula two:

Iodate N2 N-trimethyl chitosan TMC 5.0-9.0

Folium Isatidis 3.5-7.0

Radix Isatidis 7.0-9.0

Folium Eucalypti Robustae 4.0-6.0

Herba Senecionis Scandentis 4.5-7.0

Vitamin B ₁₂2.0-4.0

Vitamin B ₁₃3.0-6.0

Water to 100.0

Formula three:

Iodate N2 N-trimethyl chitosan TMC 5.0-8.0

Folium Isatidis 4.5-7.0

Radix Isatidis 7.0-8.0

Folium Eucalypti Robustae 5.0-6.0

Herba Senecionis Scandentis 4.5-6.0

Vitamin B ₁₂3.0-4.0

Vitamin B ₁₃3.0-5.0

Water to 100.0.

Claims (2)

1. the present invention relates to the special-purpose bacteria remover of a kind of carp culture, it is characterized in that having following formula (component and percentage by weight % thereof): iodate N2 N-trimethyl chitosan TMC 4.0-9.0; Folium Isatidis 3.5-8.0; Radix Isatidis 6.0-9.0; Folium Eucalypti Robustae 4.0-7.0; Herba Senecionis Scandentis 3.5-7.0; Vitamin B ₁₂2.0-5.0; Vitamin B ₁₃3.0-6.0; Water to 100.0.

2. a preparation method for the special-purpose bacteria remover of carp culture described in claim 1, is characterized in that having following step:

1) Folium Isatidis, Radix Isatidis, Folium Eucalypti Robustae, Herba Senecionis Scandentis are through grinding;

2) 60% alcoholic solution thermostat water bath warm macerating 1 ~ 2h, filters;

3) Chinese herbal medicine fluid leaching is mixed, 2000 revs/min are carried out centrifugally, extract the medicinal liquid of supernatant;

4) by iodate N2 N-trimethyl chitosan TMC, vitamin B ₁₂, vitamin B ₁₃be mixed in proportion be added to extract supernatant medicinal liquid in reactor, be uniformly mixed;

5) with sterile distilled water, be settled to desired concn, low temperature is sealed up for safekeeping standby.