CN103719645A - Method for processing Tibetan mushroom contaminating infectious microbes - Google Patents

Method for processing Tibetan mushroom contaminating infectious microbes Download PDF

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Publication number
CN103719645A
CN103719645A CN201310730513.3A CN201310730513A CN103719645A CN 103719645 A CN103719645 A CN 103719645A CN 201310730513 A CN201310730513 A CN 201310730513A CN 103719645 A CN103719645 A CN 103719645A
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CN
China
Prior art keywords
mushroom
tibet koumiss
yoghourt
mushroom body
tibet
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201310730513.3A
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Chinese (zh)
Inventor
张卫兵
薛莲
何林
马红富
陶生俭
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Gansu Agricultural University
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Gansu Agricultural University
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Filing date
Publication date
Application filed by Gansu Agricultural University filed Critical Gansu Agricultural University
Priority to CN201310730513.3A priority Critical patent/CN103719645A/en
Publication of CN103719645A publication Critical patent/CN103719645A/en
Pending legal-status Critical Current

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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L31/00Edible extracts or preparations of fungi; Preparation or treatment thereof
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L5/00Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
    • A23L5/20Removal of unwanted matter, e.g. deodorisation or detoxification

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Nutrition Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Medicines Containing Plant Substances (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

Abstract

The invention discloses a method for processing Tibetan mushroom contaminating infectious microbes. The method for processing the Tibetan mushroom contaminating infectious microbes comprises the steps of initially cleaning, soaking with normal saline, flushing with sterile water, repeatedly soaking and flushing and fermenting for producing yogurt. The method has the advantages of simple method, easiness in operation, practicability and the like. The method disclosed by the invention can be used for effectively solving the problem that contaminated Tibetan mushrooms cannot be used for fermenting to produce yogurt any more.

Description

A kind of processing method of tibet koumiss pollution microbes
technical field
The invention belongs to technical field of bioengineering, relate to a kind of processing method of tibet koumiss pollution microbes.
Background technology
" tibet koumiss " also named " hiding glossy ganoderma " or " Tibet saussurea involucrata ", is the peculiar rare bacterial classification that is derived from snowfield, Tibet.It exactly likes the grain of rice, is the block of milky, colloidal substance.The main component of tibet koumiss is water, viscous polysaccharide, a small amount of albumen, lipid and other composition, is perching the microorganisms such as galactococcus, bacillus, saccharomycete and acetic acid bacteria on mushroom grain.The Yoghourt made from tibet koumiss, long-term drinking can improve immunity, and replenishing vitamins has and delays senility, the effects such as preventing cold, treatment rheumatism, control enteric infection, fat-reducing, raising liver function, adjusting gastrointestinal function, are particularly suitable for stomach trouble, ephrosis and liver and bladder disease patient.Tibet koumiss Yoghourt extensively passes drink at folks of china.
Due to the contamination resistance of tibet koumiss a little less than, the operating process such as its inoculation while preparing Yoghourt of use among the people, fermentation all, under extensive condition, will be polluted in the course of time.Can a kind of white of growth on Yoghourt surface while occur polluting, be the miscellaneous bacteria of batting shape, be paved with Yoghourt surface, start free from extraneous odour.After a period of time, white hair shape miscellaneous bacteria color burn, frowziness produces simultaneously.The Yoghourt polluting, because quality changes, can not eat.The tibet koumiss mushroom body polluting can not continue on for Yoghourt fermentation, need to find new free of contamination mushroom body and continue to use.The processing method of a kind of tibet koumiss pollution microbes involved in the present invention, has the advantages such as method is simple, easy to operate, and the tibet koumiss mushroom body of treated mistake can continue fermented yoghourt.
Summary of the invention
The processing method that the object of this invention is to provide a kind of tibet koumiss pollution microbes.The object of the invention is to realize by following technical scheme, it is characterized in that it comprises the following steps:
(1) tentatively clean
With filter screen, the tibet koumiss mushroom body having polluted is taken out from Yoghourt, be placed in ready sterilized water 50~60 r/min vibration 1~3min, remove the Yoghourt that tibet koumiss mushroom surface is adhered to.
(2) physiological saline soaks
The tibet koumiss mushroom body of rinsing well is added in 0.9% SPSS, soak 3~5 minutes, then with filter screen, take out.
(3) aseptic water washing
By the tibet koumiss mushroom body aseptic water washing soaking, remove the physiological saline of mushroom surface.
(4) repeat to soak and rinse
Depending on pollution level, repeating step 2 and 3 operation, soak and rinse 2~3 times tibet koumiss mushroom body.
(5) Yoghourt fermentation
The tibet koumiss mushroom body of processing is inoculated in to sterilization breast with 3% inoculum concentration, 30 ℃ of fermentations 48 hours, whether adularescent hair shape miscellaneous bacteria occurs to observe fermented yoghourt surface, if there is the operation of repeating step 2 and 3, until pollution-free phenomenon occurs.
beneficial effect of the present invention
(1) processing method of tibet koumiss pollution microbes of the present invention, has the advantages such as method is simple, easy to operate, practical.
(2) the present invention effectively the tibet koumiss mushroom body of contaminated solution can not continue on for the problem of Yoghourt fermentation.
The specific embodiment
The following examples can further illustrate the present invention, but do not limit the present invention in any way.
embodiment 1
With filter screen, the tibet koumiss mushroom body of slight pollution is taken out from Yoghourt, be placed in ready sterilized water 50r/min vibration 1min, remove the Yoghourt that tibet koumiss mushroom surface is adhered to.The tibet koumiss mushroom body of rinsing well is added in 0.9% SPSS, soak 3 minutes, then with filter screen, take out.By the tibet koumiss mushroom body aseptic water washing soaking, remove the physiological saline of mushroom surface.Again the tibet koumiss mushroom body of rinsing well is added in 0.9% SPSS, soak 3 minutes, then with filter screen, take out, with aseptic water washing, to remove the physiological saline of mushroom surface.Treated tibet koumiss mushroom body is inoculated in to sterilization breast with the inoculum concentration of 3 %, and 30 ℃ of fermentations 48 hours, observe fermented yoghourt surface and occur without a white hair shape miscellaneous bacteria, and Yoghourt free from extraneous odour, illustrates that the tibet koumiss mushroom body treatment effect of pollution is better.
embodiment 2
With filter screen, the tibet koumiss mushroom body of moderate pollution is taken out from Yoghourt, be placed in ready sterilized water 55 r/min vibration 2min, remove the Yoghourt that tibet koumiss mushroom surface is adhered to.The tibet koumiss mushroom body of rinsing well is added in 0.9% SPSS, soak 4 minutes, then with filter screen, take out.By the tibet koumiss mushroom body aseptic water washing soaking, remove the physiological saline of mushroom surface.Again the tibet koumiss mushroom body of rinsing well is added in 0.9% SPSS, soak 3 minutes, then with filter screen, take out, with aseptic water washing, to remove the physiological saline of mushroom surface.The 3rd time the tibet koumiss mushroom body of rinsing well is added in 0.9% SPSS, soak 3 minutes, then with filter screen, take out, with aseptic water washing, to remove the physiological saline of mushroom surface.The tibet koumiss mushroom body of processing is inoculated in to sterilization breast with 3% inoculum concentration, and 30 ℃ of fermentations 48 hours, observe fermented yoghourt surface and occur without a white hair shape miscellaneous bacteria, and Yoghourt free from extraneous odour, illustrates that the tibet koumiss mushroom body treatment effect of pollution is better.
embodiment 3
With filter screen, the tibet koumiss mushroom body of severe contamination is taken out from Yoghourt, be placed in ready sterilized water 60 r/min vibration 3min, remove the Yoghourt that tibet koumiss mushroom surface is adhered to.The tibet koumiss mushroom body of rinsing well is added in 0.9% SPSS, soak 5 minutes, then with filter screen, take out.By the tibet koumiss mushroom body aseptic water washing soaking, remove the physiological saline of mushroom surface.Again the tibet koumiss mushroom body of rinsing well is added in 0.9% SPSS, soak 3 minutes, then with filter screen, take out, with aseptic water washing, to remove the physiological saline of mushroom surface.The 3rd time the tibet koumiss mushroom body of rinsing well is added in 0.9% SPSS, soak 3 minutes, then with filter screen, take out, with aseptic water washing, to remove the physiological saline of mushroom surface.The 4th adds the tibet koumiss mushroom body of rinsing well in 0.9% SPSS, soaks 3 minutes, then with filter screen, takes out, with aseptic water washing, to remove the physiological saline of mushroom surface.The tibet koumiss mushroom body of processing is inoculated in to sterilization breast with 3% inoculum concentration, and 30 ℃ of fermentations 48 hours, observe fermented yoghourt surface and occur without a white hair shape miscellaneous bacteria, and Yoghourt free from extraneous odour, illustrates that the tibet koumiss mushroom body treatment effect of pollution is better.

Claims (1)

1. a processing method for tibet koumiss pollution microbes, is characterized in that it comprises the following steps:
(1) tentatively clean
With filter screen, the tibet koumiss mushroom body having polluted is taken out from Yoghourt, be placed in ready sterilized water 50~60 r/min vibration 1~3min, remove the Yoghourt that tibet koumiss mushroom surface is adhered to;
(2) physiological saline soaks
The tibet koumiss mushroom body of rinsing well is added in 0.9% SPSS, soak 3~5 minutes, then with filter screen, take out;
(3) aseptic water washing
By the tibet koumiss mushroom body aseptic water washing soaking, remove the physiological saline of mushroom surface;
(4) repeat to soak and rinse
Depending on pollution level, repeating step 2 and 3 operation, soak and rinse 1~3 time tibet koumiss mushroom body;
(5) Yoghourt fermentation
The tibet koumiss mushroom body of processing is inoculated in to sterilization breast with 3% inoculum concentration, 30 ℃ of fermentations 48 hours, whether adularescent hair shape miscellaneous bacteria occurs to observe fermented yoghourt surface, if there is the operation of repeating step 2 and 3, until pollution-free phenomenon occurs.
CN201310730513.3A 2013-12-26 2013-12-26 Method for processing Tibetan mushroom contaminating infectious microbes Pending CN103719645A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201310730513.3A CN103719645A (en) 2013-12-26 2013-12-26 Method for processing Tibetan mushroom contaminating infectious microbes

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201310730513.3A CN103719645A (en) 2013-12-26 2013-12-26 Method for processing Tibetan mushroom contaminating infectious microbes

Publications (1)

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CN103719645A true CN103719645A (en) 2014-04-16

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103891889A (en) * 2014-04-24 2014-07-02 范小虎 Yoghourt for promoting digestive absorption and preparation method thereof

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101147506A (en) * 2006-09-21 2008-03-26 陆少颜 Tibetan mushroom yoghourt and its producing technique method, by-product in production course
CN101530131A (en) * 2008-04-10 2009-09-16 于岚 Method for preparing Kefir sour milk
CN102225875A (en) * 2011-05-03 2011-10-26 甘肃农业大学 Medium and cultivating method for Tibetan mushroom rapid propagation
CN102630751A (en) * 2012-05-11 2012-08-15 甘肃农业大学 Production method of Tibetan mushroom yogurt

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101147506A (en) * 2006-09-21 2008-03-26 陆少颜 Tibetan mushroom yoghourt and its producing technique method, by-product in production course
CN101530131A (en) * 2008-04-10 2009-09-16 于岚 Method for preparing Kefir sour milk
CN102225875A (en) * 2011-05-03 2011-10-26 甘肃农业大学 Medium and cultivating method for Tibetan mushroom rapid propagation
CN102630751A (en) * 2012-05-11 2012-08-15 甘肃农业大学 Production method of Tibetan mushroom yogurt

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
刘变芳 等: "自然发酵剂"西藏雪莲"的初步研究与菌种鉴定", 《中国酿造》 *
刘慧: "开菲尔粒——最安全的发酵剂", 《食品安全导刊》 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103891889A (en) * 2014-04-24 2014-07-02 范小虎 Yoghourt for promoting digestive absorption and preparation method thereof

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Application publication date: 20140416