CN103712837B - Suspension brain sheet antigen retrieval piece pressing clip - Google Patents
Suspension brain sheet antigen retrieval piece pressing clip Download PDFInfo
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- CN103712837B CN103712837B CN201310736656.5A CN201310736656A CN103712837B CN 103712837 B CN103712837 B CN 103712837B CN 201310736656 A CN201310736656 A CN 201310736656A CN 103712837 B CN103712837 B CN 103712837B
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- framework
- bedframe
- antigen retrieval
- piece pressing
- brain sheet
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Abstract
The present invention relates to the one suspension brain sheet antigen retrieval piece pressing clip in medical experiment device field, be made up of rectangular framework (3) and rectangular bedframe (4), by adjustable nut (5) framework (3) be pressed in the base groove (6) matched with framework (3) on bedframe (4); Four limits of framework (3) and four limits of bedframe (4) have diversion trench (7), and the diversion trench (7) on framework (3) and the diversion trench (7) on bedframe (4) match.Instant invention overcomes the defect that existing hot repair process easily causes suspension brain sheet generation shrinkage, the suspension brain sheet antigen retrieval piece pressing clip structure provided is simple, easy to operate, both can realize the antigen retrieval of " suspension brain sheet ", and can ensure that again brain tissue slice shrinkage did not occur.
Description
Art
The present invention relates to medical experiment device field, be specially a kind of suspension brain sheet antigen retrieval piece pressing clip.
Background technology
Suspension brain sheet is one of tissue samples commonly used in Neuroscience Research process, is used for nervous system morphology, pathological correlative study.Brain tissue sample is in manufacturing process, because the effect of chemical reagent encloses antigen, again because the effect of heat causes the peptide chain of incomplete antigen to be distorted, cause and can not display it in the dyeing course of SABC, in order to solve the above problems, the effect of heat is utilized again to be come out by these antigens or revise, be i.e. antigen retrieval.Antigen retrieval technology is one of experimental technique commonly used in the research work such as clinical examination or Senile Mouse, greatly can improve the positive rate of SABC or immunofluorescence.Wherein, " the hot repairing method of micro-wave oven " commonly uses the most, is convenient to the antigen retrieval method of extensively carrying out most.But hot repair process easily causes suspension brain sheet generation shrinkage, has a strong impact on follow-up research.
Summary of the invention
The object of the invention is the defect easily causing suspension brain sheet generation shrinkage in order to overcome existing hot repair process, there is provided a kind of structure simple, easy to operate, both can realize the antigen retrieval of " suspension brain sheet ", and can ensure that again the suspension brain sheet antigen retrieval piece pressing clip of shrinkage did not occur brain tissue slice.
The object of the invention is to be realized by following technical proposals:
Suspension brain sheet antigen retrieval piece pressing clip of the present invention, it is characterized in that piece pressing clip (1) is made up of rectangular framework (3) and rectangular bedframe (4), by adjustable nut (5) framework (3) is pressed in the base groove (6) matched with framework (3) on bedframe (4); Four limits of framework (3) and four limits of bedframe (4) have diversion trench (7), and the diversion trench (7) on framework (3) and the diversion trench (7) on bedframe (4) match.
In such scheme, described base groove (6) has slide groove (12).
In such scheme, described piece pressing clip (1) is placed in hand basket (2), hand basket (2) is by six roots of sensation cross bar (8), four vertical rods (9) and three groove poles (10) are formed, wherein, the opening surface of two cross bars (8) and two groove poles (10) composition hand basket (2), the bottom surface of four cross bars (8) composition hand basket (2), a groove pole (10) is arranged in the middle of hand basket (2) bottom surface, parallel with the groove pole (10) of two on opening surface, groove (11) on three groove poles (10) is mutually corresponding, in order to hold piece pressing clip (1), four vertical rods (9) are connected with bottom surface with the opening surface of hand basket (2) respectively.
Use procedure of the present invention is as follows:
In PBS solution (phosphate buffered solution), " suspension brain sheet " is laid on microslide, after covering another sheet microslide, be placed in the slide groove on bedframe, cover framework, rotary tight adjustable nut, microslide is clamped, is accompanied the piece pressing clip of " suspension brain sheet ".The piece pressing clip accompanying suspension brain sheet is directly immersed in the beaker filling with sodium citrate antigen retrieval buffers, or the piece pressing clip accompanying suspension brain sheet is inserted in the groove of hand basket one by one, after forming assembly, overall immersion is filled with in the beaker of sodium citrate antigen retrieval buffers, heat in micro-wave oven, high fire 5 minutes, middle high fire 20 minutes.Then at room temperature, naturally cool.After cooling, take out suspension brain sheet piece pressing clip, in PBS solution, little writing brush auxiliary under, take out the brain sheet after repairing that suspends, for follow-up Senile Mouse from microslide.
The present invention has the following advantages: structure is simple, and easy manufacture, easy to operate, effect stability, both can realize the antigen retrieval of " suspension brain sheet ", can ensure that again brain tissue slice shrinkage does not occur.
Therefore, instant invention overcomes the defect that existing hot repair process easily causes suspension brain sheet generation shrinkage, the suspension brain sheet antigen retrieval piece pressing clip structure provided is simple, easy to operate, both can realize the antigen retrieval of " suspension brain sheet ", and can ensure that again brain tissue slice shrinkage did not occur.
Accompanying drawing explanation
Fig. 1 is schematic diagram of the present invention.
Fig. 2 is hand basket schematic diagram of the present invention.
Fig. 3 is piece pressing clip of the present invention and hand basket combination schematic diagram.
In accompanying drawing, the implication of each numeral is: 1: piece pressing clip; 2: hand basket; 3: framework; 4: bedframe; 5: adjustable nut; 6: base groove; 7: diversion trench; 8: cross bar; 9: vertical rod; 10: groove pole; 11: groove; 12: slide groove; 13: screw; 14: hold-down nut.
Embodiment
Be described in further detail the present invention below in conjunction with drawings and Examples, but the present invention is not limited only to described embodiment.
Embodiment one
The suspension brain sheet antigen retrieval piece pressing clip of this example, as shown in Figure 1, is made up of rectangular framework 3 and rectangular bedframe 4, to be pressed on by upper frame 3 in the base groove 6 matched with framework 3 on bedframe 4 by adjustable nut 5; Four limits of framework 3 and four limits of bedframe 4 have diversion trench 7, and the diversion trench 7 on framework 3 and the diversion trench 7 on bedframe 4 match.
Base groove 6 has slide groove 12.
Embodiment two
The suspension brain sheet antigen retrieval piece pressing clip of this example is as Fig. 1, shown in Fig. 2 and Fig. 3, piece pressing clip 1 is with embodiment one, hand basket 2 is by six roots of sensation cross bar 8, four vertical rods 9 and three groove poles 10 are formed, wherein, two cross bars 8 and two groove poles 10 form the opening surface of hand basket 2, four cross bars 8 form the bottom surface of hand basket 2, a groove pole 10 is arranged in the middle of hand basket 2 bottom surface, parallel with the groove pole 10 of two on opening surface, groove 11 on three groove poles 10 is mutually corresponding, in order to hold piece pressing clip 1, four vertical rods 9 are connected with bottom surface with the opening surface of hand basket 2 respectively.Piece pressing clip 1 is placed in the groove 11 of hand basket 2 upper groove pole 10.
Claims (1)
1. a suspension brain sheet antigen retrieval piece pressing clip, it is characterized in that piece pressing clip (1) is made up of rectangular framework (3) and rectangular bedframe (4), by adjustable nut (5) framework (3) is pressed in the base groove (6) matched with framework (3) on bedframe (4); Four limits of framework (3) and four limits of bedframe (4) have diversion trench (7), and the diversion trench (7) on framework (3) and the diversion trench (7) on bedframe (4) match.
2. suspension brain sheet antigen retrieval piece pressing clip according to claim 1, is characterized in that described base groove (6) has slide groove (12).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310736656.5A CN103712837B (en) | 2013-12-29 | 2013-12-29 | Suspension brain sheet antigen retrieval piece pressing clip |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310736656.5A CN103712837B (en) | 2013-12-29 | 2013-12-29 | Suspension brain sheet antigen retrieval piece pressing clip |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103712837A CN103712837A (en) | 2014-04-09 |
| CN103712837B true CN103712837B (en) | 2015-10-28 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310736656.5A Expired - Fee Related CN103712837B (en) | 2013-12-29 | 2013-12-29 | Suspension brain sheet antigen retrieval piece pressing clip |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103712837B (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105751101B (en) * | 2016-03-23 | 2018-05-22 | 宁波江丰生物信息技术有限公司 | A kind of blade fixing device for being used to prevent that section from coming off |
| CN106979884B (en) * | 2017-04-20 | 2020-09-04 | 汪小明 | Urine analyzer convenient to shake reagent evenly |
| CN108931412B (en) * | 2017-05-26 | 2020-12-04 | 深圳大学 | Tissue slice antigen retrieval device and method |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5244787A (en) * | 1991-01-31 | 1993-09-14 | Biogenex Laboratories | Antigen retrieval in formalin-fixed tissues using microwave energy |
| CN2305995Y (en) * | 1997-10-17 | 1999-02-03 | 刘政国 | Antigen repairing box for microwave oven |
| CN1737527A (en) * | 2005-09-07 | 2006-02-22 | 杭州电子科技大学 | A kind of rapid installing section and thermostat |
| CN201382939Y (en) * | 2009-03-20 | 2010-01-13 | 无锡博慧斯生物医药科技有限公司 | Novel immunohistochemical experimental apparatus |
| CN201942685U (en) * | 2011-01-14 | 2011-08-24 | 刘超男 | Repair box suitable for repairing tissue antigen by microwave method |
| CN202511991U (en) * | 2012-04-10 | 2012-10-31 | 吉林金域医学检验所有限公司 | Pathological section frame convenient to move |
| CN202710381U (en) * | 2012-08-24 | 2013-01-30 | 佳木斯大学 | Anti-dropping slide device |
| CN203672691U (en) * | 2013-12-29 | 2014-06-25 | 中国人民解放军***总医院 | Pressure plate type antigen retrieval frame for suspended brain slice |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8067241B2 (en) * | 2009-08-26 | 2011-11-29 | General Electric Company | Method and apparatus for antigen retrieval process |
-
2013
- 2013-12-29 CN CN201310736656.5A patent/CN103712837B/en not_active Expired - Fee Related
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5244787A (en) * | 1991-01-31 | 1993-09-14 | Biogenex Laboratories | Antigen retrieval in formalin-fixed tissues using microwave energy |
| CN2305995Y (en) * | 1997-10-17 | 1999-02-03 | 刘政国 | Antigen repairing box for microwave oven |
| CN1737527A (en) * | 2005-09-07 | 2006-02-22 | 杭州电子科技大学 | A kind of rapid installing section and thermostat |
| CN201382939Y (en) * | 2009-03-20 | 2010-01-13 | 无锡博慧斯生物医药科技有限公司 | Novel immunohistochemical experimental apparatus |
| CN201942685U (en) * | 2011-01-14 | 2011-08-24 | 刘超男 | Repair box suitable for repairing tissue antigen by microwave method |
| CN202511991U (en) * | 2012-04-10 | 2012-10-31 | 吉林金域医学检验所有限公司 | Pathological section frame convenient to move |
| CN202710381U (en) * | 2012-08-24 | 2013-01-30 | 佳木斯大学 | Anti-dropping slide device |
| CN203672691U (en) * | 2013-12-29 | 2014-06-25 | 中国人民解放军***总医院 | Pressure plate type antigen retrieval frame for suspended brain slice |
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| Publication number | Publication date |
|---|---|
| CN103712837A (en) | 2014-04-09 |
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