CN103710268A - Application of Leptosphaerulina chartarum - Google Patents
Application of Leptosphaerulina chartarum Download PDFInfo
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- CN103710268A CN103710268A CN201310674652.9A CN201310674652A CN103710268A CN 103710268 A CN103710268 A CN 103710268A CN 201310674652 A CN201310674652 A CN 201310674652A CN 103710268 A CN103710268 A CN 103710268A
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- leaf spot
- gotu kola
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Abstract
The invention relates to a new use of a strain, and especially relates to an application of Leptosphaerulina chartarum SJTU59 in straw degradation. The preservation number of the Leptosphaerulina chartarum SJTU59 is CGMCC No.8105. Compared with strains in the prior art, the Leptosphaerulina chartarum SJTU59 has a high cellulase vitality and a high straw degradation efficiency.
Description
Technical field
The present invention relates to a kind of new purposes of bacterial strain, the particularly application of a kind of Gotu Kola leaf spot fungi (Leptosphaerulina chartarum SJTU59) aspect straw degradative.
Background technology
China is large agricultural country, and annual all kinds of agricultural crop straw output, 700,000,000 tons of left and right, occupies first place in the world.The one-tenth such as stalk three major polymers: cellulose, hemicellulose and xylogen are grouped into, and character is very stable at normal temperatures, and degradation speed is slow, so most area is because of catching up with farming season, robbing the phenomenon that crop straw burning has appearred in the reasons such as sowing.Big area crop straw burning has not only caused serious environmental pollution, and has wasted valuable Biological resources.
Microorganism can be resolved into oligosaccharides or monose by stalk by materials such as eccrine fiber element enzymes (cellulase), is the key link that occurring in nature carbon element transforms.And the features such as rate of propagation is fast, feature richness is various, strong adaptability that microorganism has, utilize micro-biological process degrading straw to have the irreplaceable advantage of many additive methods, and application prospect is very wide.
The microorganism of the degrading straw of report mainly concentrates on the bacterial strains such as genus bacillus, Trichoderma, Penicillium notatum and aspergillus tubigensis at present, it is reported first that Gotu Kola leaf spot fungi produces cellulose degraded stalk, and cellulase activity and all higher to the degradation efficiency of stalk.
Summary of the invention
The object of the invention is to provide the application of a kind of Gotu Kola leaf spot fungi aspect degrading straw, and Gotu Kola leaf spot fungi SJTU59 can efficient degradation stalk.
The object of the invention is achieved through the following technical solutions:
Gotu Kola leaf spot fungi SJTU59(Leptosphaerulina chartarum SJTU59) application aspect straw degradative, the deposit number of described Gotu Kola leaf spot fungi SJTU59 is CGMCC No.8105.
Gotu Kola leaf spot fungi SJTU59 of the present invention is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center (CGMCC), and address is No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, Institute of Microorganism, Academia Sinica.Preservation date is on August 21st, 2013, and deposit number is: CGMCC No.8105.
Gotu Kola leaf spot fungi SJTU59 of the present invention belongs to mycota, Ascomycota, and seat capsule Gammaproteobacteria, chamber Zoopagales, Dematiaceae, little bare hull belongs to, Gotu Kola leaf spot bacterial classification.
Compared with prior art, the present invention has following beneficial effect:
Gotu Kola leaf spot fungi SJTU59 cellulase activity of the present invention is higher, also higher to the degradation efficiency of stalk.
Accompanying drawing explanation
Fig. 1 is the Dynamic Curve figure of the cellulase of Gotu Kola leaf spot fungi SJTU59 generation of the present invention;
Fig. 2 is by the photo of the nutrient solution before Gotu Kola leaf spot fungi SJTU59 degraded;
Fig. 3 is by the photo of the nutrient solution after Gotu Kola leaf spot fungi SJTU59 degraded;
Fig. 4 is that the dry weight of Gotu Kola leaf spot fungi SJTU59 degrading straw of the present invention changes schematic diagram.
Embodiment
Below in conjunction with embodiment, describe the present invention in detail.
Implement the vitality test of the cellulase of 1 Gotu Kola leaf spot fungi SJTU59 generation
Preparation PD nutrient solution: 200g potato is cut into 1cm
2fritter, boil 30min, after 4 layers of filtered through gauze, retain supernatant liquor, in supernatant liquor, add 20g glucose, be settled to 1000mL, minute install in 10 250mL triangular flasks 121 ℃ of high pressure steam sterilization 21min.
Prepare the nutrient solution that corn stalk powder is sole carbon source: by 4g NH
4nO
3, 0.5g K
2hPO
4, 0.5gMgSO
47H
2o, 0.5g KCl and 0.01g FeSO
47H
2o is dissolved in 1000mL deionized water, divides and installs in 20 250mL triangular flasks, and every bottle adds 1g corn stalk powder, 121 ℃ of high pressure steam sterilization 21min.Wherein, the corn stalk powder of corn stalk powder for pulverizing and making after 80 mesh sieves filter.
The Gotu Kola leaf spot fungi SJTU59 of-80 ℃ of preservations is activated and obtains kind of a daughter bacteria in PD nutrient solution, and mycelium filters through sterile gauze, removes after large quantity of moisture, take 3:50(W/V) be transferred in the nutrient solution that corn stalk powder is sole carbon source, 28 ℃, 180rpm, cultivates 4 days.Get 1mL fermented liquid every day, 4 ℃, the centrifugal 10min of 6000prm, draws supernatant liquor and is cellulase solution.
In glass test tube, add the above-mentioned cellulase solution obtaining of 100 μ L and 1.9mL CMC-Na solution (pH4.8), fully mix, 50 ℃ of water-bath 1h.Add 3mLDNS(3,5-dinitrosalicylic acid) reagent, 100 ℃ of heating 10min, are cooled to room temperature.Under 540nm wavelength, detect absorbancy, according to typical curve, calculate cellulase activity size.CMC-Na is purchased from traditional Chinese medicines group, viscosity 800-1200pa.CMC-Na solution (pH4.8), the foundation of the preparation of DNS reagent and glucose typical curve is with reference to People's Republic of China's light industry standard (QB2583-2003).
Result shows: the cellulase activity that Gotu Kola leaf spot fungi SJTU59 produces reaches plateau at fermentation second day, 50 ℃, under pH4.8 condition, maximum enzyme vigor be 6.383 ± 0.196U/mL(as shown in Figure 1).
Implement 2 tests of Gotu Kola leaf spot fungi SJTU59 to straw degradative ability
Prepare corn stalk powder: pulverize and make corn stalk powder after 80 mesh sieves filter.
Prepare the nutrient solution that corn stalk powder is sole carbon source: by 4g NH
4nO
3, 0.5g K
2hPO
4, 0.5gMgSO
47H
2o, 0.5g KCl and 0.01g FeSO
47H
2o is dissolved in 1000mL deionized water, divides and installs in 20 250mL triangular flasks, and every bottle adds the above-mentioned corn stalk powder of 1g, 121 ℃ of high pressure steam sterilization 21min.
Kind daughter bacteria in embodiment 1 is filtered through sterile gauze, removes after large quantity of moisture, take 3:50(W/V) be transferred in the nutrient solution that corn stalk powder is sole carbon source, 28 ℃, 180rpm, cultivates 4 days.The fermentation nutrient solution of 4 days is filtered, collect corn stalk powder, remove mycelium; Repetitive scrubbing mycelium, collects the corn stalk powder that it adheres to, and avoids loss.The corn stalk powder of collecting is dried to 12h, the weight of mensuration corn stalk powder, the calculating relative dry weight of straw powder and rate of weight loss in 70 ℃.
Result shows: corn stalk powder can be degraded in a large number by Gotu Kola leaf spot fungi SJTU59, and nutrient solution color is shoaled, and slightly thick, as shown in Figure 2,3; Ferment and the degradation rate of straw powder was reached to 42%(as shown in Figure 4 in 4 days).
Visible, the vigor of the cellulase that Gotu Kola leaf spot fungi SJTU59 of the present invention produces is higher, and the efficiency of degrading straw is also higher.
Disclosed is above only several specific embodiments of the application, but the application is not limited thereto, and the changes that any person skilled in the art can think of, all should drop in the application's protection domain.
Claims (1)
1. the Gotu Kola leaf spot fungi SJTU59(Leptosphaerulina chartarum SJTU59) application aspect straw degradative, the deposit number of described Gotu Kola leaf spot fungi is CGMCC No.8105.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310674652.9A CN103710268A (en) | 2013-12-11 | 2013-12-11 | Application of Leptosphaerulina chartarum |
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| CN201310674652.9A CN103710268A (en) | 2013-12-11 | 2013-12-11 | Application of Leptosphaerulina chartarum |
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| CN103710268A true CN103710268A (en) | 2014-04-09 |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1804025A (en) * | 2005-12-09 | 2006-07-19 | 山东省中协食品添加剂研究开发中心 | Production method for xylose by enzyme process |
| CN101092639A (en) * | 2006-06-19 | 2007-12-26 | 中国农业科学院麻类研究所 | Method for degrading stalk of saccharified crops through biologic enzyme |
| CN102690022A (en) * | 2012-05-31 | 2012-09-26 | 中节能六合天融环保科技有限公司 | Upflow microbial reactor processing technology for manganese-bearing waste water |
-
2013
- 2013-12-11 CN CN201310674652.9A patent/CN103710268A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1804025A (en) * | 2005-12-09 | 2006-07-19 | 山东省中协食品添加剂研究开发中心 | Production method for xylose by enzyme process |
| CN101092639A (en) * | 2006-06-19 | 2007-12-26 | 中国农业科学院麻类研究所 | Method for degrading stalk of saccharified crops through biologic enzyme |
| CN102690022A (en) * | 2012-05-31 | 2012-09-26 | 中节能六合天融环保科技有限公司 | Upflow microbial reactor processing technology for manganese-bearing waste water |
Non-Patent Citations (2)
| Title |
|---|
| WU Q ET AL.: "Identification of a Novel Fungus, Leptosphaerulina chartarum SJTU59 and Characterization of Its Xylanolytic Enzymes", 《PLOS ONE》 * |
| 吴琼 等: "雷公根叶斑病菌SJTU59纤维素酶液的性质研究及纤维素酶基因保守区域的分析", 《中国生物工程杂志》 * |
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Application publication date: 20140409 |