CN103695513B - A kind of method improving yield of soybean peptide with low molecular weight - Google Patents
A kind of method improving yield of soybean peptide with low molecular weight Download PDFInfo
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- CN103695513B CN103695513B CN201310478523.2A CN201310478523A CN103695513B CN 103695513 B CN103695513 B CN 103695513B CN 201310478523 A CN201310478523 A CN 201310478523A CN 103695513 B CN103695513 B CN 103695513B
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- 108090000765 processed proteins & peptides Proteins 0.000 title claims abstract description 57
- 235000010469 Glycine max Nutrition 0.000 title claims abstract description 53
- 244000068988 Glycine max Species 0.000 title claims abstract description 53
- 238000000034 method Methods 0.000 title claims abstract description 42
- 239000000243 solution Substances 0.000 claims abstract description 62
- 108010073771 Soybean Proteins Proteins 0.000 claims abstract description 40
- 235000019710 soybean protein Nutrition 0.000 claims abstract description 40
- 102000004196 processed proteins & peptides Human genes 0.000 claims abstract description 37
- 239000000706 filtrate Substances 0.000 claims abstract description 26
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 22
- 238000004140 cleaning Methods 0.000 claims description 14
- 230000001954 sterilising effect Effects 0.000 claims description 13
- 239000011259 mixed solution Substances 0.000 claims description 12
- 108091005804 Peptidases Proteins 0.000 claims description 7
- 239000004365 Protease Substances 0.000 claims description 7
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims description 7
- 150000001875 compounds Chemical class 0.000 claims description 7
- 239000012528 membrane Substances 0.000 claims description 7
- 238000001471 micro-filtration Methods 0.000 claims description 7
- 238000005507 spraying Methods 0.000 claims description 7
- 238000004659 sterilization and disinfection Methods 0.000 claims description 7
- 102000004190 Enzymes Human genes 0.000 claims description 6
- 108090000790 Enzymes Proteins 0.000 claims description 6
- 238000001728 nano-filtration Methods 0.000 claims description 6
- 239000000047 product Substances 0.000 abstract description 9
- 239000003929 acidic solution Substances 0.000 abstract description 3
- 238000004519 manufacturing process Methods 0.000 abstract description 3
- 238000010521 absorption reaction Methods 0.000 abstract description 2
- 239000002537 cosmetic Substances 0.000 abstract description 2
- 230000007071 enzymatic hydrolysis Effects 0.000 abstract description 2
- 238000006047 enzymatic hydrolysis reaction Methods 0.000 abstract description 2
- 235000013305 food Nutrition 0.000 abstract description 2
- 239000007788 liquid Substances 0.000 abstract description 2
- 239000002244 precipitate Substances 0.000 abstract description 2
- 238000005516 engineering process Methods 0.000 description 3
- 108010082495 Dietary Plant Proteins Proteins 0.000 description 2
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 2
- 238000005119 centrifugation Methods 0.000 description 1
- 235000012000 cholesterol Nutrition 0.000 description 1
- 238000004332 deodorization Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 238000007701 flash-distillation Methods 0.000 description 1
- 235000004280 healthy diet Nutrition 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 238000004806 packaging method and process Methods 0.000 description 1
- 238000004091 panning Methods 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 235000018102 proteins Nutrition 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
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- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The invention discloses a kind of method improving yield of soybean peptide with low molecular weight, have employed enzyme-embrane method and deep processing has been carried out to soybean protein isolate, the yield of reacting is controlled by the pol of controlled enzymatic hydrolysis liquid, product yield is controlled by the pol and amount of water that control filtrate, product transparency is controlled by controlling concentrated solution pol, not only peptide content is high for the soybean peptides of present method production, solution is limpid bright, the output capacity of product is high, and soybean peptides molecular weight is low, easy absorption, even if also do not precipitate in an acidic solution, multiple fields such as foods and cosmetics can be widely used in.
Description
Technical field
The present invention relates to soybean protein deep process technology field, be specifically related to a kind of method improving yield of soybean peptide with low molecular weight.
Background technology
Along with the raising day by day of standard of living, the degree of concern of people to healthy diet also improves constantly.For the mode of picked-up albumen, people are no longer confined to only from panning protein in animal body, and more angles from health go out to send to absorb some containing the vegetable-protein of cholesterol, and relative to other vegetable-protein, soybean protein has the most comprehensive nutritive value.But, because soybean protein exists a lot of limitation in processing, usually soybean protein is processed into the soybean peptides that more easily can be utilized by people in practical application, meanwhile also can not affects original function in soybean protein.
At present, the manufacturing and processing enterprise of existing soybean peptides adopts following process for processing mode usually: soybean protein isolate is concentrated through dissolving, enzymolysis, centrifugation, sterilization, flash distillation deodorization, high temperature, spraying dry, packaging final prod; The soybean peptides molecular weight product that aforesaid method is produced is large, and skewness, can form precipitation in an acidic solution, and after dilution, solution colour is very dark, the inadequate clear of solution.
Based on above factor, the microfiltration membrane treatment technology that our company adopts is different from traditional centrifugal separation technique, can ensure that the molecular weight of filtrate distributes in certain scope, obtained soybean peptides solution is limpid bright, peptide content is high, and effectively can improve the output capacity of product by the pol controlling filtrate
Summary of the invention
The object of this invention is to provide a kind of method improving yield of soybean peptide with low molecular weight, not only productive rate is high for present method gained soybean peptides, and soybean peptides molecular weight is lower, and soybean peptides solution is limpid bright, and peptide content is high.
In order to realize above object, the technical solution adopted in the present invention is: a kind of method improving yield of soybean peptide with low molecular weight, comprises the following steps:
(1) take soybean protein isolate, add water, after soybean protein isolate fully dissolves, regulate soybean protein isolate pH value of solution to be 6 ~ 11;
(2) by after step (1) gained soybean protein isolate solution sterilization, add after compound protease carries out enzymolysis and obtain enzymolysis solution, conjugated protein enzyme dosage is 5 ‰ ~ 40 ‰ of soybean protein isolate weight;
(3) after the enzymolysis solution of step (2) gained being carried out microfiltration membrane process, add water cleaning filtrate, and collect the soybean peptides mixed solution leached;
(4) the soybean peptides mixed solution of step (3) gained is carried out nanofiltration operation, collect and obtain concentrated solution;
(5) by spraying dry after the concentrated solution sterilizing of step (4) gained, pulverous soybean peptides can be obtained.
The enzymolysis solution pol of described step (2) gained is 5 ~ 20BX, and pH value is 6 ~ 11.
In described step (3), filtrate pol is 4 ~ 15BX, and in cleaning process, the weight ratio of water and filtrate is 1:1 ~ 1:15.
In described step (3), filtrate pol is 15BX, and in cleaning process, the weight ratio of water and filtrate is 1:15.
In described step (4), the pol of concentrated solution is 10 ~ 35BX.
In described step (4), the pol of concentrated solution is 12BX.
The enzymolysis time of described step (2) soybean protein isolate solution is 2h ~ 10h.
The method of raising yield of soybean peptide with low molecular weight provided by the present invention, have employed enzyme-embrane method and deep processing has been carried out to soybean protein isolate, the yield of reacting is controlled by the pol of controlled enzymatic hydrolysis liquid, product yield is controlled by the pol and amount of water that control filtrate, product transparency is controlled by controlling concentrated solution pol, not only peptide content is high for the soybean peptides of present method production, solution is limpid bright, the output capacity of product is high, and soybean peptides molecular weight is low, easy absorption, even if also do not precipitate in an acidic solution, multiple fields such as foods and cosmetics can be widely used in.
Embodiment
Below by specific embodiment, technical scheme of the present invention is described in detail.
Embodiment 1
A kind of method improving yield of soybean peptide with low molecular weight that the present embodiment provides, comprises the following steps:
(1) take soybean protein isolate, add water, after soybean protein isolate fully dissolves, regulate soybean protein isolate pH value of solution to be 6;
(2) by after step (1) gained soybean protein isolate solution sterilization, add after compound protease carries out enzymolysis and obtain enzymolysis solution, conjugated protein enzyme dosage is 5 ‰ of soybean protein isolate weight; Wherein enzymolysis time is 2h, and the pol of enzymolysis solution is 5BX, and pH value is 6;
(3) after the enzymolysis solution of step (2) gained being carried out microfiltration membrane process, add water cleaning filtrate, and collect the soybean peptides mixed solution leached; Wherein the pol of gained filtrate is 4BX, and in cleaning process, the weight ratio of water and filtrate is 1:1;
(4) the soybean peptides mixed solution of step (3) gained is carried out nanofiltration operation, collect and obtain concentrated solution; Wherein the pol of the concentrated solution of gained is 10BX;
(5) by spraying dry after the concentrated solution sterilizing of step (4) gained, pulverous soybean peptides can be obtained.
Embodiment 2
A kind of method improving yield of soybean peptide with low molecular weight that the present embodiment provides, comprises the following steps:
(1) take soybean protein isolate, add water, after soybean protein isolate fully dissolves, regulate soybean protein isolate pH value of solution to be 8;
(2) by after step (1) gained soybean protein isolate solution sterilization, add after compound protease carries out enzymolysis and obtain enzymolysis solution, conjugated protein enzyme dosage is 20 ‰ of soybean protein isolate weight; Wherein enzymolysis time is 5h, and the pol of enzymolysis solution is 10BX, and pH value is 8;
(3) after the enzymolysis solution of step (2) gained being carried out microfiltration membrane process, add water cleaning filtrate, and collect the soybean peptides mixed solution leached; Wherein the pol of gained filtrate is 10BX, and in cleaning process, the weight ratio of water and filtrate is 1:5;
(4) the soybean peptides mixed solution of step (3) gained is carried out nanofiltration operation, collect and obtain concentrated solution; Wherein the pol of the concentrated solution of gained is 20BX;
(5) by spraying dry after the concentrated solution sterilizing of step (4) gained, pulverous soybean peptides can be obtained.
Embodiment 3
A kind of method improving yield of soybean peptide with low molecular weight that the present embodiment provides, comprises the following steps:
(1) take soybean protein isolate, add water, after soybean protein isolate fully dissolves, regulate soybean protein isolate pH value of solution to be 8;
(2) by after step (1) gained soybean protein isolate solution sterilization, add after compound protease carries out enzymolysis and obtain enzymolysis solution, conjugated protein enzyme dosage is 30 ‰ of soybean protein isolate weight; Wherein enzymolysis time is 8h, and the pol of enzymolysis solution is 18BX, and pH value is 8;
(3) after the enzymolysis solution of step (2) gained being carried out microfiltration membrane process, add water cleaning filtrate, and collect the soybean peptides mixed solution leached; Wherein the pol of gained filtrate is 15BX, and in cleaning process, the weight ratio of water and filtrate is 1:15;
(4) the soybean peptides mixed solution of step (3) gained is carried out nanofiltration operation, collect and obtain concentrated solution; Wherein the pol of the concentrated solution of gained is 12BX;
(5) by spraying dry after the concentrated solution sterilizing of step (4) gained, pulverous soybean peptides can be obtained.
Embodiment 4
A kind of method improving yield of soybean peptide with low molecular weight that the present embodiment provides, comprises the following steps:
(1) take soybean protein isolate, add water, after soybean protein isolate fully dissolves, regulate soybean protein isolate pH value of solution to be 11;
(2) by after step (1) gained soybean protein isolate solution sterilization, add after compound protease carries out enzymolysis and obtain enzymolysis solution, conjugated protein enzyme dosage is 40 ‰ of soybean protein isolate weight; Wherein enzymolysis time is 10h, and the pol of enzymolysis solution is 20BX, and pH value is 11;
(3) after the enzymolysis solution of step (2) gained being carried out microfiltration membrane process, add water cleaning filtrate, and collect the soybean peptides mixed solution leached; Wherein the pol of gained filtrate is 15BX, and in cleaning process, the weight ratio of water and filtrate is 1:15;
(4) the soybean peptides mixed solution of step (3) gained is carried out nanofiltration operation, collect and obtain concentrated solution; Wherein the pol of the concentrated solution of gained is 35BX;
(5) by spraying dry after the concentrated solution sterilizing of step (4) gained, pulverous soybean peptides can be obtained.
In addition, compound protease provided by the present invention is purchased from glad bio tech ltd, Shanghai hundred million.
Test example
Test according to embodiment 1 ~ 4 provided by the present invention below, and calculate the soybean peptides productive rate of each embodiment, then by after water-soluble for the soybean peptides of each embodiment gained, measure its absorbancy at 660nm place respectively, i.e. OD (660nm); In addition, embodiment 1 ~ 4 the data obtained is contrasted with the numerical value obtained according to the method test provided in " soybean is separated the applied research of enzymic hydrolysis and peptide " (hereinafter referred to as method 1) paper, specifically sees form 1.
The correlation data of form 1 the present invention and currently known methods output soybean peptides
Can see from table 1, the soybean peptides productive rate of embodiment 3 provided by the invention up to 70.31%, far away higher than the soybean peptides productive rate 45.50% of current method 1; In addition, after water-soluble for the soybean peptides of embodiment 3 gained, the absorbancy at the 660nm place measured is also high compared with the absorbancy of the soybean peptides of method 1 gained.Therefore, the soybean peptides molecular weight that the present invention produces is low, easily absorbs, and the output capacity of product is high, and gained solution is limpid bright.
Claims (4)
1. improve a method for yield of soybean peptide with low molecular weight, it is characterized in that, comprise the following steps:
(1) take soybean protein isolate, add water, after soybean protein isolate fully dissolves, regulate soybean protein isolate pH value of solution to be 6 ~ 11;
(2) by after step (1) gained soybean protein isolate solution sterilization, add after compound protease carries out enzymolysis and obtain enzymolysis solution, conjugated protein enzyme dosage is 5 ‰ ~ 40 ‰ of soybean protein isolate weight;
(3) after the enzymolysis solution of step (2) gained being carried out microfiltration membrane process, add water cleaning filtrate, and collect the soybean peptides mixed solution leached;
(4) the soybean peptides mixed solution of step (3) gained is carried out nanofiltration operation, collect and obtain concentrated solution;
(5) by spraying dry after the concentrated solution sterilizing of step (4) gained, pulverous soybean peptides can be obtained;
The enzymolysis solution pol of described step (2) gained is 5 ~ 20BX, pH value is 6 ~ 11, in described step (3), filtrate pol is 4 ~ 15BX, in cleaning process, the weight ratio of water and filtrate is 1:1 ~ 1:15, and in described step (4), the pol of concentrated solution is 10 ~ 35BX.
2. the method for raising yield of soybean peptide with low molecular weight according to claim 1, is characterized in that, in described step (3), filtrate pol is 15BX, and in cleaning process, the weight ratio of water and filtrate is 1:15.
3. the method for raising yield of soybean peptide with low molecular weight according to claim 1, is characterized in that, in described step (4), the pol of concentrated solution is 12BX.
4. the method for raising yield of soybean peptide with low molecular weight according to claim 1, is characterized in that, the enzymolysis time of described step (2) soybean protein isolate solution is 2h ~ 10h.
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| CN105614899B (en) * | 2016-01-13 | 2019-02-01 | 刘新旗 | A kind of low molecule soybean peptide and preparation method thereof |
| CN107674900B (en) * | 2017-06-12 | 2021-05-14 | 诺利如一（安阳）生物科技有限公司 | Process method for concentrating soybean peptide by adopting tandem membrane |
| CN108208309A (en) * | 2017-12-28 | 2018-06-29 | 保龄宝生物股份有限公司 | The production method of low ash content soybean peptide containing profitable probliotics |
| CN114480546A (en) * | 2022-03-01 | 2022-05-13 | 佳木斯冬梅大豆食品有限公司 | Method for improving yield of low-molecular-weight soybean peptide |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101974589A (en) * | 2010-10-29 | 2011-02-16 | 江南大学 | Method for preparing immunocompetent soybean peptide by enzymolysis and membrane separation |
| CN103146788A (en) * | 2013-02-28 | 2013-06-12 | 珠海博康药业有限公司 | Enzymic-method based preparation method of soybean peptide |
| CN103343154A (en) * | 2013-07-19 | 2013-10-09 | 东北农业大学 | Preparation of soybean anti-fatigue biological active peptide |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101974589A (en) * | 2010-10-29 | 2011-02-16 | 江南大学 | Method for preparing immunocompetent soybean peptide by enzymolysis and membrane separation |
| CN103146788A (en) * | 2013-02-28 | 2013-06-12 | 珠海博康药业有限公司 | Enzymic-method based preparation method of soybean peptide |
| CN103343154A (en) * | 2013-07-19 | 2013-10-09 | 东北农业大学 | Preparation of soybean anti-fatigue biological active peptide |
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Inventor after: Liu Xinqi Inventor after: Fu Hui Yan Inventor after: Zhang Haiping Inventor after: Guo Ji Inventor before: Yue Bingxiang Inventor before: Fu Hui Yan Inventor before: Zhang Haiping Inventor before: Zhang Xinwei |
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Effective date of registration: 20160531 Address after: Tangyin County of Anyang City, the 456150 industrial agglomeration in Henan Province four Street on the south side of the road on the west side of the longitudinal work area Jingjhong Patentee after: NUOLI RUYI (ANYANG) BIOLOGICAL SCIENCE & TECHNOLOGY CO.,LTD. Patentee after: NUOLI RUYI (BEIJING) BIOLOGICAL TECHNOLOGY CO.,LTD. Address before: Tangyin County of Anyang City, the 456150 industrial agglomeration in Henan Province four Street on the south side of the road on the west side of the longitudinal work area Jingjhong Patentee before: NUOLI RUYI (ANYANG) BIOLOGICAL SCIENCE & TECHNOLOGY CO.,LTD. |
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Address after: 455000 West Road, 100 meters south of the intersection of Jingzhong Road and Xinglong Road in Tangyin County, Anyang City, Henan Province Patentee after: NUOLI RUYI (ANYANG) BIOLOGICAL SCIENCE & TECHNOLOGY CO.,LTD. Patentee after: Shanggu Heyi (Zhenjiang) Food Technology Co.,Ltd. Address before: 456150 west side of gongzongsi street, south side of Jingzhong Road, industrial agglomeration zone, Tangyin County, Anyang City, Henan Province Patentee before: NUOLI RUYI (ANYANG) BIOLOGICAL SCIENCE & TECHNOLOGY CO.,LTD. Patentee before: RONGHAI BIOTECH CO.,LTD. |