CN103675169A - Method for filling gel chromatographic columns - Google Patents
Method for filling gel chromatographic columns Download PDFInfo
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- CN103675169A CN103675169A CN201210320062.1A CN201210320062A CN103675169A CN 103675169 A CN103675169 A CN 103675169A CN 201210320062 A CN201210320062 A CN 201210320062A CN 103675169 A CN103675169 A CN 103675169A
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Abstract
The invention discloses a method for filling gel chromatographic columns. The method comprises the following steps: preparing uniform slurry, namely adding gel powder into deionized water, fully wetting, then heating and boiling for 2-3 hours so as to obtain the uniform slurry; (2) connecting a filling system, namely connecting the upper end of a hollow column tube onto a uniform-slurry tank, and connecting a stainless-steel capillary tube with the inner diameter being 0.2-0.6mm with the lower end of the hollow column tube; (3) filling the deionized water with the volume being about 1/3 of the volume of the hollow column tube, and then slowly adding the uniform slurry into the uniform slurry tank; (4) starting an air compressor, pressing into displacing liquid, and maintaining for 20-180 minutes under the pressure of 5.5-8.5MPa, wherein the displacing liquid is a mixture of water and methyl alcohol; and (5) washing a column bed with the deionized water as displacing liquid with the amount being 1-3 times of the volume of the column, so as to prepare the gel chromatographic columns. The method disclosed by the invention has the advantages that according to the gel chromatographic columns prepared by using the method, bubbles in fillers can be eliminated, and the column bed can be closely filled with the fillers, so that the efficiency of the chromatographic columns is obviously improved, and simultaneously, the success rate for filling the chromatographic columns is increased from 76% to more than 90%.
Description
Technical field
The present invention relates to a kind of method of loading gel column, more specifically, the present invention relates to the method for a kind of filling polymer gel high pressure liquid chromatography (HPLC) post.
Background technology
Exclusion chromatography is called again molecular exclusion chromatography, and it is a kind of quick, the simple separate analytical technique that eighties of last century early sixties grows up, and is mainly used in relative molecular mass hierarchical analysis and the relative molecular mass distribution tests of superpolymer.As the critical component of exclusion chromatography, the tradition of organic polymer gel chromatographic columns dress column method comprises machinery dress post method and people's frock post method.Machinery dress post method is in crossing current situation, directly gel slurry is filled in post, owing to there is no external pressure, so filler is difficult to load closelyr, thereby causes the post effect of chromatographic column low, the life-span is short; People's frock column method, dress post is unstable, often can not be once success, often need often refitting, not only workload is large, and the life-span of chromatographic column and post effect not high yet.
For the problems referred to above, the present inventor has studied the packing method of silica gel chromatographic column in earnest, and its filling device as shown in Figure 1, by improving the filling process of silica gel chromatographic column, and has completed the present invention on the basis of great many of experiments.
Summary of the invention
According to an aspect of the present invention, provide a kind of method of loading gel chromatographic columns, the method comprises the steps:
(1) prepare homogenate: gel powder is added in deionized water, and after fully soaking, heating is boiled 2～3 hours, obtains homogenate;
(2) connect loading system: the upper end of void column pipe is connected on homogenate tank, and connects in void column pipe lower end the stainless steel capillary that internal diameter is 0.2～0.6mm;
(3) in void column pipe, inject the deionized water that is about void column pipe volume 1/3, then described homogenate is added in homogenate tank lentamente;
(4) start air compressor machine, be pressed into displacement fluid, and keep 20～80 minutes under the pressure of 5.5～8.5MPa, wherein this displacement fluid is the potpourri of water and methyl alcohol; And
(5) use deionized water instead as displacement fluid, and rinse post bed with the amount of 1～3 times of column volume, make gel chromatographic columns.
In a preferred embodiment, the weight ratio of the gel powder described in above-mentioned steps (1) and deionized water is 1: 3～4.
In another preferred embodiment, above-mentioned steps (1) also comprises that filtering floats on the fine particle of the water surface, then makes the step of homogenate natural subsidence, and repeats this step 2～4 time.
In another preferred embodiment, in the potpourri of the water described in above-mentioned steps (4) and methyl alcohol, the volume ratio of water and methyl alcohol is 3: 1～1: 3.
In a preferred embodiment again, aforementioned gel powder is cross-link dextran.
In a preferred embodiment again, the weight-average molecular weight of this cross-link dextran is 1000～200000.
In a preferred embodiment again, this cross-link dextran is to be selected from a kind of in following: Sephadex G-10, Sephadex G-15, Sephadex G-25, Sephadex G-50, Sephadex G-75, Sephadex G-100, and Sephadex G-150.
Experiment shows that prepared in accordance with the method for the present invention gel chromatographic columns not only can be eliminated the bubble in filler; Organic polymer filler is closely filled on post bed; And improved widely the consistance of filling work.Therefore, the chromatographic column that the method according to this invention obtains has higher column efficiency and longer serviceable life, also makes the success ratio of loading chromatographic column be brought up to more than 90% by 76% of classic method simultaneously.
Accompanying drawing explanation
In order to understand better design of the present invention, feature and advantage, hereinafter with reference to the accompanying drawings, be described more specifically the present invention.In the accompanying drawings:
Fig. 1 is the schematic diagram of the device of existing filling silica gel chromatographic column; And
Fig. 2 is the schematic diagram that is filled with the device of organic polymer gel chromatographic columns of the present invention.
Embodiment
Consideration is owing to not applying external pressure in the filling of existing organic polymer gel chromatographic columns, so first the inventor expects the packing method filling polymer gel chromatographic column with silica gel chromatographic column.And, for the polymer gel of filling is applied to larger pressure, in one embodiment of the invention, under the filter plate of the empty chromatographic column lower end of the inventor in silicon gel chromatographic columns filling device, installed the approximately stainless steel capillary of 1～5mm of an internal diameter additional, limited the outflow of solvent, thereby the pressure in the time of can increasing filling polymer gel in chromatographic column, makes polymer gel can load closelyr, and then improve the efficiency of chromatographic column.
In another embodiment, the inventor attempt with system ratio be the water of 3: 1～1: 3 and methyl alcohol as displacement fluid, the methyl alcohol or the ethanol that replace classic method to adopt, found that, can make like this polymer gel load closelyr.
In yet another embodiment, inventor changes traditional homogenate preparation method, but after in gel powder is added to deionized water, is heated and boils 2～3 hours.It is believed that so not only can to make gel powder swelling abundant, and can get rid of gas wherein, and kill bacterium and/or the microorganism that wherein may exist, thereby make there is no bubble in prepared chromatographic column filler, and extend its serviceable life.
In an embodiment again, inventor is also in the preparation process of homogenate, after having boiled, filtering floats on the fine particle of the water surface, then makes its natural subsidence, then filtering floats on the fine particle of the water surface, after stirring again, then except floating on the fine particle of the water surface.Such filtering process repeats 2 ~ 4 times.It is believed that such operation can guarantee the consistance of homogenate, and then guarantee the consistance of dress column operation, and then improved the success ratio of filling chromatographic column.Experiment shows, adopts method of the present invention, can make the success ratio of loading chromatographic column be brought up to more than 90% by 76% of classic method.
In an embodiment again, the polymer gel powder that method of the present invention is used is cross-link dextran, and its weight-average molecular weight is generally 1000～200000, is preferably 10000～150000.For instance, the Sephadex G-10 that can use AM General company to produce, Sephadex G-15, Sephadex G-25, Sephadex G-50, Sephadex G-75, Sephadex G-100, or Sephadex G-150.
In addition, inject the deionized water that is about void column pipe volume 1/3 in void column pipe, can prevent from producing when homogenate from entering chromatographic column bubble.
Now will, by the following examples, further illustrate the preferred embodiments of the invention.
Except otherwise herein provided, otherwise the meaning that the scientific and technical terminology of using herein or device name are all understood conventionally with general technical staff of the technical field of the invention is identical.
Embodiment 1
Get Sephadex G-10 gel filler (production of U.S. GE company) 35g and put into 500ml beaker, add the deionized water that 300ml is fresh, beaker is put into boiling water bath and boil 2 hours, then place and spend the night, standby as gel slurry;
Chromatographic column void column pipe (specification is internal diameter 7.8mm, length 300mm) is received on 115ml homogenate tube to termination one internal diameter 0.3mm, length 20cm stainless steel capillary under column jecket; In chromatographic column blank pipe, inject the distilled water of 1/3 volume, then by making standby gel slurry, pour into slowly in homogenate tank.In displacement tank (flask volume is 5L), fill methanol/water ratio and be the displacement fluid (the void column pipe that this place uses, homogenate tank and stainless steel capillary are all that American I dex company produces) of 1: 3.
Start pneumatic amplification pump, pressure is pressurized to 6.7MPa gradually since 0, and the solvent in homogenate and displacement fluid is flowed out from column jecket lower end, continue after 30 minutes, pressure release lower prop, then use deionized water rinsing twice column volume, sephadex column finished product.
Use water as mobile phase and measure properties of product, specimen in use is blue dextran 2000, and measurement result is:
Post effect (theoretical cam curve) N=1200, hangover Tf=1.3, and do not change after sample introduction 20 pins.
Embodiment 2
Get Sephadex G-10 gel filler (production of U.S. GE company) 35g and put into 500ml beaker, add the deionized water that 300ml is fresh, beaker is put into boiling water bath and boil 2 hours, then place and spend the night, standby as gel slurry;
Chromatographic column void column pipe (specification is internal diameter 7.8mm, length 300mm) is received on 115ml homogenate tube to termination one internal diameter 0.2mm, length 10cm stainless steel capillary under column jecket; In chromatographic column blank pipe, inject the distilled water of 1/3 volume, then by making standby gel slurry, pour into slowly in homogenate tank.In displacement tank (flask volume is 5L), fill methanol/water ratio and be the displacement fluid (the void column pipe that this place uses, homogenate tank and stainless steel capillary are all that American I dex company produces) of 2: 3.
Start pneumatic amplification pump, pressure is pressurized to 6.7MPa gradually since 0, and the solvent in homogenate and displacement fluid is flowed out from column jecket lower end, continue after 60 minutes, pressure release lower prop, then use deionized water rinsing twice column volume, sephadex column finished product.
Use water as mobile phase and measure properties of product, specimen in use is blue dextran 2000, and measurement result is:
Post effect (theoretical cam curve) N=1230, hangover Tf=1.2, and do not change after sample introduction 20 pins.
Embodiment 3
Get Sephadex G-25 gel filler (production of U.S. GE company) 35g and put into 500ml beaker, add the deionized water that 300ml is fresh, beaker is put into boiling water bath and boil 2 hours, then place and spend the night, standby as gel slurry;
Chromatographic column void column pipe (specification is internal diameter 7.8mm, length 300mm) is received on 115ml homogenate tube to termination one internal diameter 0.3mm, length 30cm stainless steel capillary under column jecket; In chromatographic column blank pipe, inject the distilled water of 1/3 volume, then by making standby gel slurry, pour into slowly in homogenate tank.In displacement tank (flask volume is 5L), fill methanol/water ratio and be the displacement fluid (the void column pipe that this place uses, homogenate tank and stainless steel capillary are all that American I dex company produces) of 1: 3.
Start pneumatic amplification pump, pressure is pressurized to 7.0MPa gradually since 0, and the solvent in homogenate and displacement fluid is flowed out from column jecket lower end, continue after 30 minutes, pressure release lower prop, then use deionized water rinsing twice column volume, sephadex column finished product.
Use water as mobile phase and measure properties of product, specimen in use is blue dextran 2000, and measurement result is:
Post effect (theoretical cam curve) N=1270, hangover Tf=1.2, and do not change after sample introduction 20 pins.
Embodiment 4
Get Sephadex G-50 gel filler (production of U.S. GE company) 35g and put into 500ml beaker, add the deionized water that 300ml is fresh, beaker is put into boiling water bath and boil 2 hours, then place and spend the night, standby as gel slurry;
Chromatographic column void column pipe (specification is internal diameter 7.8mm, length 300mm) is received on 115ml homogenate tube to termination one internal diameter 0.15mm, length 15cm stainless steel capillary under column jecket; In chromatographic column blank pipe, inject the distilled water of 1/3 volume, then by making standby gel slurry, pour into slowly in homogenate tank.In displacement tank (flask volume is 5L), fill methanol/water ratio and be the displacement fluid (the void column pipe that this place uses, homogenate tank and stainless steel capillary are all that American I dex company produces) of 2: 1.
Start pneumatic amplification pump, pressure is pressurized to 8.0MPa gradually since 0, and the solvent in homogenate and displacement fluid is flowed out from column jecket lower end, continue after 60 minutes, pressure release lower prop, then use deionized water rinsing twice column volume, sephadex column finished product.
Use water as mobile phase and measure properties of product, specimen in use is blue dextran 2000, and measurement result is:
Post effect (theoretical cam curve) N=1310, hangover Tf=1.1, and do not change after sample introduction 20 pins.
Embodiment 5
Get Sephadex G-100 gel filler (production of U.S. GE company) 35g and put into 500ml beaker, add the deionized water that 300ml is fresh, beaker is put into boiling water bath and boil 2 hours, then place and spend the night, standby as gel slurry;
Chromatographic column void column pipe (specification is internal diameter 7.8mm, length 300mm) is received on 115ml homogenate tube to termination one internal diameter 0.3mm, length 20cm stainless steel capillary under column jecket; In chromatographic column blank pipe, inject the distilled water of 1/3 volume, then by making standby gel slurry, pour into slowly in homogenate tank.In displacement tank (flask volume is 5L), fill methanol/water ratio and be the displacement fluid (the void column pipe that this place uses, homogenate tank and stainless steel capillary are all that American I dex company produces) of 3: 1.
Start pneumatic amplification pump, pressure is pressurized to 8.2MPa gradually since 0, and the solvent in homogenate and displacement fluid is flowed out from column jecket lower end, continue after 100 minutes, pressure release lower prop, then use deionized water rinsing twice column volume, sephadex column finished product.
Use water as mobile phase and measure properties of product, specimen in use is blue dextran 2000, and measurement result is:
Post effect (theoretical cam curve) N=1220, hangover Tf=1.3, and do not change after sample introduction 20 pins.
The result of above-described embodiment shows, efficiency and the life-span of the organic polymer gel chromatographic columns of preparing by method of the present invention have all obtained significant raising.
Although described for illustrative purposes typical embodiment, above stated specification should not be construed the restriction to this paper scope.Therefore,, within not departing from spirit and scope herein, those skilled in the art can carry out various improvement, modification and replacement.
Claims (7)
1. a method of loading gel chromatographic columns, comprises the steps:
(1) prepare homogenate: gel powder is added in deionized water, and after fully soaking, heating is boiled 2～3 hours, obtains homogenate;
(2) connect loading system: the upper end of void column pipe is connected on homogenate tank, and connects in void column pipe lower end the stainless steel capillary that internal diameter is 0.2～0.6mm;
(3) in void column pipe, inject the deionized water that is about void column pipe volume 1/3, then described homogenate is added in homogenate tank lentamente;
(4) start air compressor machine, be pressed into displacement fluid, and keep 20～180 minutes under the pressure of 5.5～8.5MPa, wherein this displacement fluid is the potpourri of water and methyl alcohol; And
(5) use deionized water instead as displacement fluid, and rinse post bed with the amount of 1～3 times of column volume, make gel chromatographic columns.
2. according to the method for the filling gel chromatographic columns of claim 1, wherein the weight ratio of the gel powder described in step (1) and deionized water is 1: 3～4.
3. according to the method for the filling gel chromatographic columns of claim 1, wherein step (1) also comprises that filtering floats on the fine particle of the water surface, then makes the step of homogenate natural subsidence, and repeats this step 2～4 time.
4. according to the method for the filling gel chromatographic columns of claim 1, wherein, in the potpourri of the water described in step (4) and methyl alcohol, the volume ratio of water and methyl alcohol is 3: 1～1: 3.
5. according to the method for the filling gel chromatographic columns of any one in aforementioned claim, wherein said gel powder is cross-link dextran.
6. according to the method for the filling gel chromatographic columns of claim 5, the weight-average molecular weight of wherein said cross-link dextran is 1000～200000.
7. according to the method for the filling gel chromatographic columns of claim 5, wherein said cross-link dextran is to be selected from a kind of in following: Sephadex G-10, Sephadex G-15, Sephadex G-25, Sephadex G-50, Sephadex G-75, Sephadex G-100, and Sephadex G-150.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105158379A (en) * | 2015-08-19 | 2015-12-16 | 中国检验认证集团湖北有限公司 | Gel chromatographic column for detecting PBDEs (poly brominated diphenyl ethers) and hydroxyl metabolites of PBDEs through HPLC-MS as well as detection method of gel chromatographic column |
| CN106442822A (en) * | 2015-08-05 | 2017-02-22 | 无锡加莱克色谱科技有限公司 | Homogenate tank filled with high performance liquid chromatographic column, filling device and filling method |
| CN110531012A (en) * | 2018-05-25 | 2019-12-03 | 中科院大连化学物理研究所淮安化工新材料研究中心 | Different phosphorus content C18 liquid phases half prepare chromatography column packing method |
| CN112986465A (en) * | 2021-02-23 | 2021-06-18 | 厦门色谱分析仪器有限公司 | Multilayer assembling preparation method of chromatographic analysis column |
| CN113009056A (en) * | 2021-02-23 | 2021-06-22 | 晋江精纯科技有限公司 | Method and device for filling multi-column series-connected chromatographic column |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS6362506A (en) * | 1986-09-03 | 1988-03-18 | Showa Denko Kk | Column packing device for liquid chromatography |
| JPS63100375A (en) * | 1986-10-16 | 1988-05-02 | Hitachi Plant Eng & Constr Co Ltd | Method and apparatus for packing into column for chromatography |
| JPH04323557A (en) * | 1991-04-23 | 1992-11-12 | Hitachi Plant Eng & Constr Co Ltd | Device for filling column for chromatography |
| CN2911683Y (en) * | 2006-07-11 | 2007-06-13 | 北京丰杰华信科学仪器有限公司 | Gel chromatographic column |
| CN201417267Y (en) * | 2009-06-03 | 2010-03-03 | 大连依利特分析仪器有限公司 | Column packing device of liquid chromatography column |
-
2012
- 2012-08-31 CN CN201210320062.1A patent/CN103675169A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS6362506A (en) * | 1986-09-03 | 1988-03-18 | Showa Denko Kk | Column packing device for liquid chromatography |
| JPS63100375A (en) * | 1986-10-16 | 1988-05-02 | Hitachi Plant Eng & Constr Co Ltd | Method and apparatus for packing into column for chromatography |
| JPH04323557A (en) * | 1991-04-23 | 1992-11-12 | Hitachi Plant Eng & Constr Co Ltd | Device for filling column for chromatography |
| CN2911683Y (en) * | 2006-07-11 | 2007-06-13 | 北京丰杰华信科学仪器有限公司 | Gel chromatographic column |
| CN201417267Y (en) * | 2009-06-03 | 2010-03-03 | 大连依利特分析仪器有限公司 | Column packing device of liquid chromatography column |
Non-Patent Citations (7)
| Title |
|---|
| JM SOSA: "Chromatography with Sephadex gels", 《ANAL. CHEM.》 * |
| P.A.NEDDERMEYER 等: "Gel filtration behavior of inorganic salts", 《ANAL. CHEM.》 * |
| 刘铭祥 等: "用凝胶色谱法研究木素的分子量分布及其应用", 《中国造纸》 * |
| 李爱民 等: "液相色谱交联聚苯乙烯固定相的填充", 《色谱》 * |
| 李瑞萍 等: "高效制备液相色谱柱技术的研究进展", 《化学进展》 * |
| 王璞 等: "凝胶过滤层析实验中Sephadex的溶胀与回收保存", 《实验技术与管理》 * |
| 赵继红 等: "高效液相色谱柱的稀匀浆填充方法", 《北方工业大学学报》 * |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106442822A (en) * | 2015-08-05 | 2017-02-22 | 无锡加莱克色谱科技有限公司 | Homogenate tank filled with high performance liquid chromatographic column, filling device and filling method |
| CN106442822B (en) * | 2015-08-05 | 2018-06-12 | 无锡加莱克色谱科技有限公司 | Load homogenate tank, filling device and the method for performance liquid chromatographic column |
| CN105158379A (en) * | 2015-08-19 | 2015-12-16 | 中国检验认证集团湖北有限公司 | Gel chromatographic column for detecting PBDEs (poly brominated diphenyl ethers) and hydroxyl metabolites of PBDEs through HPLC-MS as well as detection method of gel chromatographic column |
| CN105158379B (en) * | 2015-08-19 | 2017-06-20 | 中国检验认证集团湖北有限公司 | A kind of method based on gel chromatography post detection PBDE Metabolism of hydroxyl content |
| CN110531012A (en) * | 2018-05-25 | 2019-12-03 | 中科院大连化学物理研究所淮安化工新材料研究中心 | Different phosphorus content C18 liquid phases half prepare chromatography column packing method |
| CN112986465A (en) * | 2021-02-23 | 2021-06-18 | 厦门色谱分析仪器有限公司 | Multilayer assembling preparation method of chromatographic analysis column |
| CN113009056A (en) * | 2021-02-23 | 2021-06-22 | 晋江精纯科技有限公司 | Method and device for filling multi-column series-connected chromatographic column |
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Application publication date: 20140326 |