CN103674934B - Micro-fluidic chip Image-forming instrument and system for chemiluminescence detection - Google Patents
Micro-fluidic chip Image-forming instrument and system for chemiluminescence detection Download PDFInfo
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- CN103674934B CN103674934B CN201310503116.2A CN201310503116A CN103674934B CN 103674934 B CN103674934 B CN 103674934B CN 201310503116 A CN201310503116 A CN 201310503116A CN 103674934 B CN103674934 B CN 103674934B
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- 238000002038 chemiluminescence detection Methods 0.000 title claims abstract description 26
- 238000001514 detection method Methods 0.000 claims abstract description 41
- 230000003287 optical effect Effects 0.000 claims abstract description 36
- 238000003384 imaging method Methods 0.000 claims description 34
- 238000000034 method Methods 0.000 claims description 11
- 239000000126 substance Substances 0.000 claims description 2
- 238000005516 engineering process Methods 0.000 description 11
- 238000004458 analytical method Methods 0.000 description 10
- 238000003018 immunoassay Methods 0.000 description 7
- 239000007788 liquid Substances 0.000 description 7
- 229940088597 hormone Drugs 0.000 description 6
- 239000005556 hormone Substances 0.000 description 6
- 102000012673 Follicle Stimulating Hormone Human genes 0.000 description 3
- 108010079345 Follicle Stimulating Hormone Proteins 0.000 description 3
- 102000018997 Growth Hormone Human genes 0.000 description 3
- 108010051696 Growth Hormone Proteins 0.000 description 3
- 102000009151 Luteinizing Hormone Human genes 0.000 description 3
- 108010073521 Luteinizing Hormone Proteins 0.000 description 3
- 102000003946 Prolactin Human genes 0.000 description 3
- 108010057464 Prolactin Proteins 0.000 description 3
- 102000011923 Thyrotropin Human genes 0.000 description 3
- 108010061174 Thyrotropin Proteins 0.000 description 3
- 239000003153 chemical reaction reagent Substances 0.000 description 3
- 201000010099 disease Diseases 0.000 description 3
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 3
- 229940028334 follicle stimulating hormone Drugs 0.000 description 3
- 239000000122 growth hormone Substances 0.000 description 3
- 230000010354 integration Effects 0.000 description 3
- 229940040129 luteinizing hormone Drugs 0.000 description 3
- 238000012986 modification Methods 0.000 description 3
- 230000004048 modification Effects 0.000 description 3
- 229940097325 prolactin Drugs 0.000 description 3
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 2
- 238000003556 assay Methods 0.000 description 2
- 238000004140 cleaning Methods 0.000 description 2
- 239000011248 coating agent Substances 0.000 description 2
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- 102000007562 Serum Albumin Human genes 0.000 description 1
- 108010071390 Serum Albumin Proteins 0.000 description 1
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- 230000007547 defect Effects 0.000 description 1
- 239000006185 dispersion Substances 0.000 description 1
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- 230000007613 environmental effect Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- HWYHZTIRURJOHG-UHFFFAOYSA-N luminol Chemical compound O=C1NNC(=O)C2=C1C(N)=CC=C2 HWYHZTIRURJOHG-UHFFFAOYSA-N 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000004204 optical analysis method Methods 0.000 description 1
- 239000008363 phosphate buffer Substances 0.000 description 1
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- 238000012360 testing method Methods 0.000 description 1
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- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
The invention discloses a kind of micro-fluidic chip Image-forming instrument and system for chemiluminescence detection, the instrument includes apparatus frame and the light path stuck-module on the apparatus frame, chip support structures, illuminator and optical lens group and detection module, the light path stuck-module is used for fixing the position of optical lens group and the adjustment of light path and fixation, the chip support structures are used for supporting and moving chip is to adjust chip position, and the illuminator and optical lens group are used for collecting the chemiluminescence signal on chip and focusing on the detection module；The detection module is used for receiving chemiluminescence signal and being changed into analog electrical signal, then analog electrical signal is changed into digital electric signal.The instrument connection computer constitutes system.The instrument can carry out effectively collecting and analyzing to the chemiluminescence signal on micro-fluidic chip, and the equipment instrument is little, is a kind of new portable micro-fluidic chip analyzer.
Description
Technical Field
The invention relates to the technical field of microfluidic chip analysis systems, in particular to a microfluidic chip imaging instrument and a microfluidic chip imaging system for chemiluminescence detection.
Background
The microfluidic analysis method is a technology newly developed in recent years, mainly based on analytical chemistry, combines the achievements of relevant subjects such as biochemistry, molecular biology, physical chemistry and immunology, controls nanoliter-to-picoliter volume fluid in a micron-sized structure, analyzes and detects substances to be detected, and has the advantages of small volume, large specific surface area, short reaction time, high analysis speed, less reagent and sample consumption, simultaneous detection of multiple samples and multiple indexes and the like. Has important practical value for the discovery of relevant disease markers and early diagnosis of diseases in clinical disease research. Conventional diagnostic assays require relatively long assay times, cumbersome liquid handling procedures, relatively low throughput (only one or a few samples can be tested at a time), and relatively high antibody reagents. The microfluidic analysis chip can effectively overcome the defects. For example, when a sample with a very small sample size is analyzed, the microfluidic technology has a strong advantage, and an experiment generally requiring a sample size of a few milliliters needs only a sample size of a few microliters after the microfluidic technology is adopted, so that the consumption of the sample and the reagent is greatly saved. In the field of clinical immunoassay, the combination of microfluidic analysis technology and immunoassay can overcome other disadvantages of traditional immunoassay to a certain extent, so that the microfluidic immunoassay technology has attracted extensive attention of clinical examination medicine in recent years. The application of the microfluidic technology to immunoassay can greatly promote the improvement of the immunoassay technology, and has wide application prospect and important application value.
However, in the current microfluidic immunoassay method, although the microfluidic chip has a small volume, the detection instrument matched with the microfluidic chip has a large volume, which is not convenient for the wide application and popularization of the microfluidic detection method. But also can not fully reflect the advantages of microfluidics in the aspect of simple and rapid detection. Mainly because there is no special instrument for detecting the micro-fluidic chip, it is necessary to develop a small micro-fluidic chip analyzer to realize the organic integration of signal acquisition and analysis processing, so as to miniaturize and simplify the micro-fluidic technology.
Chemiluminescence is optical radiation generated in a chemical reaction process, and a chemiluminescence analysis method is an analysis method established by means of chemiluminescence phenomenon, does not need a light source and a dispersion device, does not have interference of common scattered light and stray light in an optical analysis method, greatly improves the signal to noise ratio, has the advantages of good specificity and wide linear range, and is widely applied to various fields of life science, clinical medicine, environmental detection and the like in recent years. In the immunodetection in hospitals, the chemiluminescence method of the micropore plate becomes a main detection method and plays a great role in clinical diagnosis work. After the micro-fluidic chip technology is combined with the chemiluminescence detection method, the requirements of the micro-fluidic chip on the detection device can be effectively reduced, the volume of the detection device is reduced, and the popularization and the application of the micro-fluidic chip technology are facilitated. Therefore, there is a need in the art for a small instrument specifically for microfluidic chip detection that can combine microfluidic chip technology with chemiluminescence detection methods.
Disclosure of Invention
The invention provides a micro-fluidic chip imaging instrument and a system for chemiluminescence detection, which can effectively collect and analyze chemiluminescence signals on a micro-fluidic chip, have small volume and are novel portable micro-fluidic chip analyzers.
The invention provides the following technical scheme:
a micro-fluidic chip imaging instrument for chemiluminescence detection comprises an instrument frame, and a light path fixing module, a chip supporting structure, a reflector, an optical lens group and a detection module which are arranged on the instrument frame, wherein the light path fixing module is used for fixing the position of the optical lens group and adjusting and fixing a light path, the chip supporting structure is used for supporting and moving a chip to adjust the position of the chip, and the reflector and the optical lens group are used for collecting and focusing a chemiluminescence signal on the chip to the detection module; the detection module is used for receiving the chemiluminescence signal, converting the chemiluminescence signal into an analog electric signal, and converting the analog electric signal into a digital electric signal.
Preferably, the chip support structure is a chip tray, more preferably a push-pull chip tray.
Preferably, the detection module is a CCD camera.
Preferably, the CCD camera is horizontally disposed.
Preferably, the instrument frame is a light tight structure.
Preferably, the imaging apparatus further comprises a switching power supply.
Preferably, the detection module is connected to a computer, and the computer receives and processes the digital electrical signal from the detection module.
Preferably, the imaging apparatus is cuboid in shape as a whole.
Preferably, the length of three adjacent sides of the cuboid-shaped imaging apparatus is 12-18cm, 12-18cm and 35-45cm, preferably 15cm, 15cm and 40cm, respectively.
A micro-fluidic chip imaging system for chemiluminescence detection comprises the micro-fluidic chip imaging instrument for chemiluminescence detection and a computer connected with the micro-fluidic chip imaging instrument.
The invention has the beneficial effects that: the invention relates to a micro-fluidic chip imaging instrument for chemiluminescence detection, which comprises an instrument frame, and a light path fixing module, a chip supporting structure, a reflector, an optical lens group and a detection module which are arranged on the instrument frame, wherein the light path fixing module is used for fixing the position of the optical lens group and adjusting and fixing a light path, the chip supporting structure is used for supporting and moving a chip to adjust the position of the chip, and the reflector and the optical lens group are used for collecting and focusing a chemiluminescence signal on the chip to the detection module; the detection module is used for receiving the chemiluminescence signal, converting the chemiluminescence signal into an analog electric signal, and converting the analog electric signal into a digital electric signal. The imaging instrument with the structure can effectively collect and analyze the chemiluminescence signals on the microfluidic chip. The imaging instrument is specially used for the chemiluminescence signal of the microfluidic chip, and has no other irrelevant components except the main components; moreover, the observation area is relatively small, so that focusing is not needed, and the optical path is small; the reflector changes the direction of the light path to enable the detection module (CCD camera) to be horizontally arranged, and the overall height of the analyzer is reduced, so that the analyzer is small in size and is a novel portable microfluidic chip analyzer.
Drawings
Fig. 1 is a schematic structural diagram of a microfluidic chip imaging system for chemiluminescence detection of the present invention, wherein 1 denotes an instrument frame, 2 denotes an optical path fixing module, 3 denotes a chip tray, 4 denotes a mirror, 5 denotes an optical lens group, 6 denotes a CCD camera, 7 denotes a switching power supply, and 8 denotes a computer.
Fig. 2 is a schematic diagram of a chemiluminescence signal optical path in a microfluidic chip imaging instrument for chemiluminescence detection, wherein 1 represents an instrument frame, 2 represents an optical path fixing module, 3 represents a chip tray, 4 represents a reflector, 5 represents an optical lens group, 6 represents a CCD camera, 7 represents a switching power supply, and 9 represents a microfluidic chip.
Fig. 3 is a photograph of the microfluidic immunodetection result read by the microfluidic chip imaging system for chemiluminescence detection of the present invention, wherein a white square represents a chemiluminescence signal, and a brighter white square represents a stronger chemiluminescence signal.
Detailed Description
Embodiments of the present invention will be described in detail with reference to examples. It will be appreciated by those skilled in the art that the following examples are only preferred embodiments of the invention to facilitate a better understanding of the invention and therefore should not be taken as limiting the scope of the invention. Various modifications and changes may be made by those skilled in the art, and any modification, equivalent replacement or improvement made without departing from the spirit and principle of the present invention should be covered within the protection scope of the present invention.
Referring to fig. 1 and 2, the microfluidic chip imaging system for chemiluminescence detection includes an imaging instrument and a computer 8. The Device mainly comprises a Device frame 1, a light path fixing module 2, a chip tray 3, a reflector 4, an optical lens group 5, a Charge-coupled Device (CCD) camera 6 and a switching power supply 7. The instrument frame 1 is used for fixing the positions of all components of the instrument; the optical path fixing module 2 is used for fixing the position of the optical lens group 5 and adjusting and fixing the optical path; the chip tray 3 is used for moving the chip and adjusting the position of the chip, so that a chemiluminescence signal area of the chip is arranged right below an observation area of the detection system; the reflector 4 and the optical lens group 5 are used for collecting a chemiluminescence signal on the chip, and the CCD camera 6 is used for collecting the chemiluminescence signal on the chip; the CCD camera 6 converts the obtained optical signal into an analog electrical signal, and then converts the analog electrical signal into a digital electrical signal to be transmitted to the computer 8. The system realizes the integration of a detection system, meets the requirement of carrying out chemiluminescence signal detection on the microfluidic chip 9, has small volume, is a novel portable microfluidic chip analyzer, and has important application potential in the field of microfluidic analysis.
The chip tray 3 can be replaced by other chip supporting structures such as a chip supporting plate, but the chip tray 3 is preferred in the invention, and a push-pull type chip tray is particularly preferred, the push-pull type chip tray enables the chip to be taken and placed very conveniently, and the chip tray is pulled out to place the chip on the chip tray and then pushed in. The CCD camera 6 may be replaced by another detection module, as long as the detection module can receive the chemiluminescent signal, convert the chemiluminescent signal into an analog electrical signal, and convert the analog electrical signal into a digital electrical signal. The CCD camera 6 of the present invention is small in size and horizontally arranged, thereby reducing the overall height of the instrument. The collection of the chemiluminescence signals needs a light-proof environment to eliminate the interference of light in the environment, so that the instrument frame is of a light-tight structure. The imaging instrument of the invention is cuboid, the lengths of three adjacent sides can be 12-18cm, 12-18cm and 35-45cm respectively, for example, the width can be 12cm, 13cm, 14cm, 15cm, 16cm, 17cm or 18cm, preferably 15 cm; the height may be 12cm, 13cm, 14cm, 15cm, 16cm, 17cm or 18cm, preferably 15 cm; the length may be 35cm, 37cm, 39cm, 41cm, 43cm or 45cm, preferably 40 cm. It can be seen that the imaging apparatus is very compact and convenient to use and carry.
The working principle and the process of the micro-fluidic chip imaging system are as follows: the chip tray 3 is pulled out, the microfluidic chip 9 is placed on the tray, and the chip tray 3 is pushed in, so that the influence of external light is avoided; the chemiluminescence signal of the microfluidic chip 9 is reflected by the reflector 4 and then collected by the optical lens group 5; the CCD camera 6 converts the optical signal irradiated to the surface of the CCD chip into an electrical signal, which is then transmitted to the computer 8 for data processing and analysis.
Through tests, the micro-fluidic chip imaging instrument has higher resolution and sensitivity, and can meet the requirement of performing chemiluminescence method detection on the micro-fluidic chip; the instrument has simple and compact structure, small volume and convenient carrying and use. The microfluidic chemiluminescence detection system has the following characteristics: the chip tray adopts a push-pull design, has good light shading performance, is convenient for putting in and taking out the chip, can accurately determine the spatial position of the chip and is convenient for detection; the reflector is used, so that the light path is changed in a right angle, the CCD camera can be horizontally placed, and the space is utilized to the maximum extent; the system is simple in design and low in manufacturing cost; the integration of the detection system is realized, the volume is small, and the field detection is convenient.
The chemiluminescence signal of the microfluidic chip is detected by using the microfluidic chip imaging system. Specifically, coating antibodies of Thyroid Stimulating Hormone (TSH), Prolactin (PRL), Luteinizing Hormone (LH), Growth Hormone (GH), and Follicle Stimulating Hormone (FSH) are previously immobilized in the 1, 2, 3, 4, and 5 directions of the microfluidic chip, respectively; and (3) sequentially introducing a sealing liquid (calf serum albumin and the like), hormones (the 5 hormones, the cleaning liquid (phosphate buffer liquid and the like), a mixed hormone detection antibody (an antibody mixed liquid of the 5 hormones, the cleaning liquid and a chemiluminescent liquid (hydrogen peroxide, luminol and the like) into the A, B, C, D and E five microfluidic pipelines, wherein the hormones introduced into the A, B, C, D and E five microfluidic pipelines are TSH, PRL, LH, GH and FSH. The photograph read (fig. 3) shows: the 5 hormones can be captured by the specific coating antibody and detected by the detection antibody, wherein white squares represent chemiluminescence signals, and the brighter the white squares, the stronger the chemiluminescence signals.
The applicant declares that the present invention is described by the above embodiments as the detailed features and the detailed methods of the present invention, but the present invention is not limited to the above detailed features and the detailed methods, that is, it is not meant that the present invention must be implemented by relying on the above detailed features and the detailed methods. It will be apparent to those skilled in the art that any modification of the present invention, equivalent substitutions of selected components of the present invention, additions of auxiliary components, selection of specific modes and the like, are within the scope and disclosure of the present invention.
Claims (9)
1. A micro-fluidic chip imaging instrument for chemiluminescence detection comprises an instrument frame and a detection module arranged on the instrument frame; wherein the instrument frame is an optically sealed structure; the optical instrument is characterized by further comprising an optical path fixing module, a push-pull chip tray, a reflector and an optical lens group, wherein the optical path fixing module, the push-pull chip tray, the reflector and the optical lens group are installed on the instrument frame; wherein,
the reflector, the optical lens group, the light path fixing module and the detection module are positioned above the internal space structure of the instrument frame; the reflector, the optical lens group, the light path fixing module and the detection module are sequentially arranged from left to right;
the optical path fixing module is used for fixing the position of the optical lens group and adjusting and fixing the optical path;
the push-pull chip tray is positioned below the instrument frame and used for supporting and moving the chip to adjust the position of the chip so as to enable a chemiluminescent signal area of the chip to be positioned right below the observation area;
the reflector and the optical lens group are used for collecting and focusing a chemiluminescence signal on a chip to the detection module; the chemical luminous signals of the chip are collected through the optical lens group after being reflected by the reflector;
the detection module is used for receiving the chemiluminescence signal, converting the chemiluminescence signal into an analog electric signal, and converting the analog electric signal into a digital electric signal.
2. The microfluidic chip imaging instrument for chemiluminescence detection according to claim 1, wherein the detection module is a CCD camera.
3. The microfluidic chip imaging instrument for chemiluminescence detection according to claim 2, wherein the CCD camera is horizontally disposed.
4. The microfluidic chip imaging instrument for chemiluminescence detection according to claim 1, further comprising a switching power supply.
5. The microfluidic chip imaging instrument for chemiluminescence detection according to claim 1, wherein the detection module is connected to a computer, and the computer receives and processes digital electrical signals from the detection module.
6. The microfluidic chip imaging instrument for chemiluminescence detection according to any one of claims 1 to 5, wherein the whole body has a rectangular parallelepiped shape.
7. A microfluidic chip imaging instrument for chemiluminescence detection according to claim 6, wherein the length of three adjacent sides of the cuboid is 12-18cm, 12-18cm and 35-45cm respectively.
8. The microfluidic chip imaging instrument for chemiluminescence detection according to claim 7, wherein the length of three adjacent sides of the cuboid is 15cm, 15cm and 40cm, respectively.
9. A microfluidic chip imaging system for chemiluminescence detection, comprising the microfluidic chip imaging apparatus for chemiluminescence detection according to any one of claims 1-8 and a computer connected thereto.
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| CN201310503116.2A CN103674934B (en) | 2013-10-23 | 2013-10-23 | Micro-fluidic chip Image-forming instrument and system for chemiluminescence detection |
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| CN105388131A (en) * | 2014-09-09 | 2016-03-09 | 国家纳米科学中心 | Fluorescence detection instrument and system based on micro-fluidic chip |
| CN104535547B (en) * | 2014-11-29 | 2017-04-05 | 北京工业大学 | A kind of common focused light passages portable unit of observation micro-fluidic chip |
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| CN201859122U (en) * | 2010-09-13 | 2011-06-08 | 北京赛智创业科技有限公司 | Integrated micro chemical light-emitting imager |
| CN202049111U (en) * | 2011-01-25 | 2011-11-23 | 山东师范大学 | Laser-induced fluorescent detector for micro-fluidic analysis |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201859122U (en) * | 2010-09-13 | 2011-06-08 | 北京赛智创业科技有限公司 | Integrated micro chemical light-emitting imager |
| CN202049111U (en) * | 2011-01-25 | 2011-11-23 | 山东师范大学 | Laser-induced fluorescent detector for micro-fluidic analysis |
Non-Patent Citations (2)
| Title |
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| Microfluidic biochip for chemiluminescent detection of allergen-specific antibodies;Kecin A.Heyries 等;《Biosensors and Bioelectronics》;20080307;1802-1808 * |
| 微流控芯片化学发光检测***的关键技术研究;卢绍永;《中国优秀硕士学位论文全文数据库 信息科技辑》;20111215;正文18-33页 * |
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