CN103667130A - Phytophthora culture medium and preparation method thereof - Google Patents
Phytophthora culture medium and preparation method thereof Download PDFInfo
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- CN103667130A CN103667130A CN201310651986.4A CN201310651986A CN103667130A CN 103667130 A CN103667130 A CN 103667130A CN 201310651986 A CN201310651986 A CN 201310651986A CN 103667130 A CN103667130 A CN 103667130A
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- rice
- epidemic disease
- water
- phytophthora
- sunflower seed
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Abstract
The present invention provides a phytophthora culture medium and a preparation method thereof, wherein 20-40 g of rice is added to 400-600 ml of water, water boiling bath heating is performed for 10-20 min to obtain rice water, the rice water is mixed with 400-600 ml of a soil leaching solution and 4-6 g of sunflower seed powder, and sterilization is performed to obtain the finished product. According to the present invention, the phytophthora culture medium provides a good culture effect for phytophthora, and the number of the zoosporangium of the phytophthora is more; and the phytophthora culture medium preparation method has characteristics of simpleness, wide raw material source, and simple and easily-available raw material, and is suitable for industrial production preparation.
Description
Technical field
The invention belongs to bacteria culture medium formulation art, relate in particular to mould substratum of a kind of epidemic disease and preparation method thereof.
Background technology
The cultivation of phytophthora is now all generally to adopt rolled oats to add water, boils and gets filtrate, and then packing sterilizing obtains.But the zoosporangium of the phytophthora of cultivating with this medium oatmeal is fewer.
Summary of the invention
The object of the present invention is to provide the mould substratum of a kind of epidemic disease, be intended to solve the existing medium oatmeal problem undesirable to phytophthora culture effect.
The present invention is achieved in that the mould substratum of a kind of epidemic disease, comprises soil extract, sunflower seed powder, rice, and wherein, the volume mass of described soil extract, sunflower seed powder and rice is than being (400～600) ml:(4～6) g:(20～40) g.
Preferably, the volume mass of described soil extract, sunflower seed powder and rice is than being 500ml:5g:30g.
Preferably, the pH value of the mould substratum of described epidemic disease is 5.5～7.5.
The preparation method who the present invention further provides the mould substratum of a kind of epidemic disease, comprises the following steps:
(1) rice is added to water, and the container that fills water and rice is placed in to boiling water heats 10～20min, mixed solution in described container after heating is filtered and obtains thin rice gruel; Wherein, the mass volume ratio of described rice and water is (20～40) g:(400～600) ml;
(2) soil extract, sunflower seed powder are joined respectively in the thin rice gruel that step (1) makes, after sterilising treatment, obtain the mould substratum of epidemic disease; Wherein, the mass volume ratio of described soil extract, sunflower seed powder and thin rice gruel is (400～600) ml:(4～6) g:(400～600) ml.
The invention provides mould substratum of a kind of epidemic disease and preparation method thereof, (20～40) g rice is added in (400～600) ml water, and boiled after 10～20min is heated in bath and obtained thin rice gruel by water, then after this thin rice gruel is mixed with (400～600) ml soil extract, (4～6) g sunflower seed powder, sterilizing prepares.The mould substratum of epidemic disease of the present invention is good to the culture effect of phytophthora, and the zoosporangium of phytophthora is more; The preparation method of the mould substratum of epidemic disease provided by the invention is simple, and starting material wide material sources are simple and easy to get, are suitable for industrialization manufacture.
Embodiment
In order to make object of the present invention, technical scheme and advantage clearer, below in conjunction with embodiment, the present invention is further elaborated.Should be appreciated that specific embodiment described herein, only in order to explain the present invention, is not intended to limit the present invention.
Embodiment 1
A preparation method for the mould substratum of epidemic disease, comprises the following steps:
(1) 20g rice is added to 500ml water, and the container that fills water and rice is placed in to boiling water heats 15min, mixed solution in described container after heating is filtered and obtains thin rice gruel;
(2) 600ml soil extract, 6g sunflower seed powder are joined respectively in the thin rice gruel that step (1) makes, after sterilising treatment, obtain the mould substratum 1 of epidemic disease.
Embodiment 2
(1) 40g rice is added to 400ml water, and the container that fills water and rice is placed in to boiling water heats 20min, mixed solution in described container after heating is filtered and obtains thin rice gruel;
(2) 400ml soil extract, 4g sunflower seed powder are joined respectively in the thin rice gruel that step (1) makes, after sterilising treatment, obtain the mould substratum 2 of epidemic disease.
Embodiment 3
(1) 30g rice is added to 500ml water, and the container that fills water and rice is placed in to boiling water heats 10min, mixed solution in described container after heating is filtered and obtains thin rice gruel;
(2) 500ml soil extract, 5g sunflower seed powder are joined respectively in the thin rice gruel that step (1) makes, after sterilising treatment, obtain the mould substratum 3 of epidemic disease.
Comparative example
(1) 30g rice is added to 500ml water, and will fill the container heated and boiled 10min of water and rice, mixed solution in described container after heating is filtered and obtains thin rice gruel;
(2) 500ml soil extract, 5g sunflower seed powder are joined respectively in the thin rice gruel that step (1) makes, after sterilising treatment, obtain the mould substratum 4 of epidemic disease.
Compare the shortcoming and defect with prior art, the present invention has following beneficial effect: the mould substratum of epidemic disease of the present invention is good to the culture effect of phytophthora, and the zoosporangium of phytophthora is more; The preparation method of the mould substratum of epidemic disease provided by the invention is simple, and starting material wide material sources are simple and easy to get, are suitable for industrialization manufacture.
The foregoing is only preferred embodiment of the present invention, not in order to limit the present invention, all any modifications of doing within the spirit and principles in the present invention, be equal to and replace and improvement etc., within all should being included in protection scope of the present invention.
Claims (4)
1. the mould substratum of epidemic disease, is characterized in that, comprises soil extract, sunflower seed powder, rice, and wherein, the volume mass of described soil extract, sunflower seed powder and rice is than being (400～600) ml:(4～6) g:(20～40) g.
2. the mould substratum of epidemic disease as claimed in claim 1, is characterized in that, the volume mass of described soil extract, sunflower seed powder and rice is than being 500ml:5g:30g.
3. the mould substratum of epidemic disease as claimed in claim 2, is characterized in that, the pH value of the mould substratum of described epidemic disease is 5.5～7.5.
4. a preparation method for the mould substratum of epidemic disease, is characterized in that, comprises the following steps:
(1) rice is added to water, and the container that fills water and rice is placed in to boiling water heats 10～20min, mixed solution in described container after heating is filtered and obtains thin rice gruel; Wherein, the mass volume ratio of described rice and water is (20～40) g:(400～600) ml;
(2) soil extract, sunflower seed powder are joined respectively in the thin rice gruel that step (1) makes, after sterilising treatment, obtain the mould substratum of epidemic disease; Wherein, the mass volume ratio of described soil extract, sunflower seed powder and thin rice gruel is (400～600) ml:(4～6) g:(400～600) ml.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310651986.4A CN103667130A (en) | 2013-12-06 | 2013-12-06 | Phytophthora culture medium and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310651986.4A CN103667130A (en) | 2013-12-06 | 2013-12-06 | Phytophthora culture medium and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103667130A true CN103667130A (en) | 2014-03-26 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201310651986.4A Pending CN103667130A (en) | 2013-12-06 | 2013-12-06 | Phytophthora culture medium and preparation method thereof |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110819583A (en) * | 2018-08-10 | 2020-02-21 | 云南农业大学 | Spore-producing culture method of phytophthora sojae |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1991000002A1 (en) * | 1989-06-29 | 1991-01-10 | Maui Shiitake Trading Company, Inc. | Substrate and method for culture of fungi, including shiitake (lentinus edodes) |
-
2013
- 2013-12-06 CN CN201310651986.4A patent/CN103667130A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1991000002A1 (en) * | 1989-06-29 | 1991-01-10 | Maui Shiitake Trading Company, Inc. | Substrate and method for culture of fungi, including shiitake (lentinus edodes) |
Non-Patent Citations (3)
| Title |
|---|
| 杨军 等: "烟草根际土壤浸出液刺激烟草疫霉产孢和接种研究", 《中国烟草学报》, vol. 17, no. 2, 30 April 2011 (2011-04-30), pages 71 - 74 * |
| 王惠哲 等: "黄瓜根腐病致病病原的鉴定", 《华北农学报》, vol. 18, no. 2, 31 December 2003 (2003-12-31), pages 74 - 77 * |
| 黄芳 等: "西葫芦根腐病的病原鉴定", 《山西农业科学》, vol. 35, no. 12, 31 December 2007 (2007-12-31), pages 28 - 30 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110819583A (en) * | 2018-08-10 | 2020-02-21 | 云南农业大学 | Spore-producing culture method of phytophthora sojae |
| CN110819583B (en) * | 2018-08-10 | 2021-07-06 | 云南农业大学 | Spore-producing culture method of phytophthora sojae |
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Application publication date: 20140326 |
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