CN103656126A - Application and preparation method of cholagogic tablet - Google Patents
Application and preparation method of cholagogic tablet Download PDFInfo
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- CN103656126A CN103656126A CN201310698944.6A CN201310698944A CN103656126A CN 103656126 A CN103656126 A CN 103656126A CN 201310698944 A CN201310698944 A CN 201310698944A CN 103656126 A CN103656126 A CN 103656126A
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Abstract
The invention provides an application of a cholagogic tablet in preparation of a drug for inhibiting mouse sarcoma S180 cell proliferation. A preparation method of the cholagogic tablet comprises the steps as follows: 58 g of rheum officinale, 58 g of honeysuckles, 58 g of longhairy antenoron herbs, 96.5 g of radix aucklandiae, 58 g of rhizoma anemarrhenae, 58 g of folium isatidis, 58 g of Chinese thorowax root, 58 g of white peony roots, 29 g of scutellaria baicalensis, 19 g of mirabilite and 58 g of oriental wormwood are taken and prepared into the cholagogic tablet according to a conventional process with a water extraction method.
Description
Technical field
The present invention relates to Chinese medicine preparation technical field, be specifically related to a kind of application and preparation method of cholagogic tablet.
Background technology
Cholagogic tablet is recorded in Ministry of Public Health standard, preparation method is: get Radix Et Rhizoma Rhei 58g, Flos Lonicerae 58g, Herba Lysimachiae 58g, Radix Aucklandiae 96.5g, Rhizoma Anemarrhenae 58g, Folium Isatidis 58g, Radix Bupleuri 58g, Radix Paeoniae Alba 58g, Radix Scutellariae 29g, Natrii Sulfas 19g, Herba Artemisiae Scopariae 58g, above ten simply, Radix Et Rhizoma Rhei, Flos Lonicerae, Herba Lysimachiae, Radix Aucklandiae powder is broken into fine powder, the Rhizoma Anemarrhenae, Folium Isatidis, Radix Bupleuri, the Radix Paeoniae Alba, Radix Scutellariae, Herba Artemisiae Scopariae decocts with water twice, filter, merging filtrate, standing 12 hours, get supernatant standby, Natrii Sulfas adds 2 times of water, heating for dissolving, filter, filtrate adds in supernatant, mix, be condensed into thick paste shape, add above-mentioned fine powder to granulate, be pressed into 1000, sugar coating, obtain, in prior art, not yet there is it in preparation, to suppress the application report in S180 mice sarcoma cell propagation medicine.
Summary of the invention
Goal of the invention: in order to address the above problem, the object of the present invention is to provide a kind of application and preparation method of cholagogic tablet.
Technical scheme: the object of the invention is to realize by following scheme:
Cholagogic tablet suppresses the application in S180 mice sarcoma cell propagation medicine in preparation, the preparation method of cholagogic tablet is: get Radix Et Rhizoma Rhei 58g, Flos Lonicerae 58g, Herba Lysimachiae 58g, Radix Aucklandiae 96.5g, Rhizoma Anemarrhenae 58g, Folium Isatidis 58g, Radix Bupleuri 58g, Radix Paeoniae Alba 58g, Radix Scutellariae 29g, Natrii Sulfas 19g, Herba Artemisiae Scopariae 58g, above ten simply, Radix Et Rhizoma Rhei, Flos Lonicerae, Herba Lysimachiae, Radix Aucklandiae powder is broken into fine powder, the Rhizoma Anemarrhenae, Folium Isatidis, Radix Bupleuri, the Radix Paeoniae Alba, Radix Scutellariae, Herba Artemisiae Scopariae decocts with water twice, filter, merging filtrate, standing 12 hours, get supernatant standby, Natrii Sulfas adds 2 times of water, heating for dissolving, filter, filtrate adds in supernatant, mix, be condensed into thick paste shape, add above-mentioned fine powder to granulate, be pressed into 1000, sugar coating, obtain.
Above-mentioned cholagogic tablet suppresses the application in S180 mice sarcoma cell propagation medicine in preparation, decocts with water twice, and the 8-12 that adds for the first time water and be medical material weight doubly measures, and decocts 1-2h, and the 6-10 that adds for the second time water and be medical material weight doubly measures, and decocts 1-2h.
Beneficial effect: cholagogic tablet can suppress S180 mice sarcoma cell propagation, can be for the preparation of suppressing S180 mice sarcoma cell propagation medicine.
The specific embodiment
Form by the following examples, foregoing of the present invention is described in further detail again, but this should be interpreted as to the scope of the above-mentioned theme of the present invention only limits to following example, all technology realizing based on foregoing of the present invention all belong to scope of the present invention.
Embodiment 1
Get Radix Et Rhizoma Rhei 58g, Flos Lonicerae 58g, Herba Lysimachiae 58g, Radix Aucklandiae 96.5g, Rhizoma Anemarrhenae 58g, Folium Isatidis 58g, Radix Bupleuri 58g, Radix Paeoniae Alba 58g, Radix Scutellariae 29g, Natrii Sulfas 19g, Herba Artemisiae Scopariae 58g, above ten simply, Radix Et Rhizoma Rhei, Flos Lonicerae, Herba Lysimachiae, Radix Aucklandiae powder is broken into fine powder, the Rhizoma Anemarrhenae, Folium Isatidis, Radix Bupleuri, the Radix Paeoniae Alba, Radix Scutellariae, Herba Artemisiae Scopariae decocts with water twice, adding for the first time water is 8 times of amounts of medical material weight, decoct 1h, adding for the second time water is 6 times of amounts of medical material weight, decoct 1h, filter, merging filtrate, standing 12 hours, get supernatant standby, Natrii Sulfas adds 2 times of water, heating for dissolving, filter, filtrate adds in supernatant, mix, be condensed into thick paste shape, add above-mentioned fine powder to granulate, be pressed into 1000, sugar coating, obtain.
Embodiment 2
Get Radix Et Rhizoma Rhei 58g, Flos Lonicerae 58g, Herba Lysimachiae 58g, Radix Aucklandiae 96.5g, Rhizoma Anemarrhenae 58g, Folium Isatidis 58g, Radix Bupleuri 58g, Radix Paeoniae Alba 58g, Radix Scutellariae 29g, Natrii Sulfas 19g, Herba Artemisiae Scopariae 58g, above ten simply, Radix Et Rhizoma Rhei, Flos Lonicerae, Herba Lysimachiae, Radix Aucklandiae powder is broken into fine powder, the Rhizoma Anemarrhenae, Folium Isatidis, Radix Bupleuri, the Radix Paeoniae Alba, Radix Scutellariae, Herba Artemisiae Scopariae decocts with water twice, adding for the first time water is 10 times of amounts of medical material weight, decoct 1.5h, adding for the second time water is 8 times of amounts of medical material weight, decoct 1.5h, filter, merging filtrate, standing 12 hours, get supernatant standby, Natrii Sulfas adds 2 times of water, heating for dissolving, filter, filtrate adds in supernatant, mix, be condensed into thick paste shape, add above-mentioned fine powder to granulate, be pressed into 1000, sugar coating, obtain.
Embodiment 3
Get Radix Et Rhizoma Rhei 58g, Flos Lonicerae 58g, Herba Lysimachiae 58g, Radix Aucklandiae 96.5g, Rhizoma Anemarrhenae 58g, Folium Isatidis 58g, Radix Bupleuri 58g, Radix Paeoniae Alba 58g, Radix Scutellariae 29g, Natrii Sulfas 19g, Herba Artemisiae Scopariae 58g, above ten simply, Radix Et Rhizoma Rhei, Flos Lonicerae, Herba Lysimachiae, Radix Aucklandiae powder is broken into fine powder, the Rhizoma Anemarrhenae, Folium Isatidis, Radix Bupleuri, the Radix Paeoniae Alba, Radix Scutellariae, Herba Artemisiae Scopariae decocts with water twice, adding for the first time water is 12 times of amounts of medical material weight, decoct 2h, adding for the second time water is 10 times of amounts of medical material weight, decoct 2h, filter, merging filtrate, standing 12 hours, get supernatant standby, Natrii Sulfas adds 2 times of water, heating for dissolving, filter, filtrate adds in supernatant, mix, be condensed into thick paste shape, add above-mentioned fine powder to granulate, be pressed into 1000, sugar coating, obtain.
Embodiment 4: cholagogic tablet suppresses the experimentation data of S180 mice sarcoma cell propagation
1 experiment material
1.1 experiment cell strains
S180 mice sarcoma cell, Nanjing Medical University's laboratory cell bank, DMEM+10%FBS cellar culture.
1.2 Experimental agents
Drugs: cholagogic tablet of the present invention: press embodiment 3 method preparations.
Medicinal liquid liquid storage: take 100mg cholagogic tablet, be dissolved in 5ml dehydrated alcohol, 0.2 μ m filter filters, 500 μ l doff pipe subpackages ,-20 ℃ of storages, 0.2 μ m filter filters dehydrated alcohol in order to the use of matched group simultaneously.
1.3 experiment reagent
The Cat.No.12100-061Lot.No.758137 of DMEM(GIBCO company); Hyclone (Lot.No.100419 of Tian Hang bio tech ltd, Zhejiang); The NaHCO3(Shanghai Jiu Yi chemical reagent Cat.No.11810-033Lot.No.1088387 of company limited); Trypsin(AMRESCO company lot number: 2010/04); EDTA(AMRESCO company lot number: 2009/10); Penicillin G Sodium Salt(AMRESCO company lot number: 2010242); Streptomycin Sulfate(AMRESCO company lot number: 2010382); Dehydrated alcohol (Nanjing Chemistry Reagent Co., Ltd.'s lot number: 080310182); MTT (Biosharp lot number: 0793); The autogamy of PBS(laboratory);
1.4 experiment equipment
Lycra inverted microscope (German Leica model: DM1L); Visible-ultraviolet light microwell plate detector (U.S. MD company model: SPECTRA MAX190); CO2 incubator (FORMA model: 3111); (safe and sound company of Su Jing group manufactures model to super-clean bench: SW-CJ-ZFD); Pure water instrument (U.S. Spring company model: S/N020579); Accurate pipettor (French Gilson Inc model: P2); Electronic balance (German Sai Duolisi company limited model: BT323S); Full-automatic high-pressure autoclave (Japanese SANYO company model: MLS-3020); Table electrothermal air dry oven (Shanghai accurate experimental facilities company model: DHG9123A); Refrigerator (Siemens Company's model: KG18V21TI); Liquid nitrogen container (CBS model: 2001); Low speed centrifuge (Anting Scientific Instrument Factory, Shanghai's model: KA-1000); 0.2 μ m filter (MILLIPORE model: SLGP033RB); 10cm culture dish (NEST company), 96 well culture plates (NEST company); Cell counting count board; Centrifuge tube, pipet, Tips are some.
2 experimental techniques
1) S180 mice sarcoma cell carries out cellar culture (10cm culture dish) with DMEM+10%FBS in 37 ℃, 5%CO2, when Growth of Cells is during to logarithmic (log) phase, collecting cell, discards culture fluid, PBS fine laundering 3 times, add 3ml0.25% trypsin-0.04%EDTA, after 37 ℃ of digestion 2min, add wherein 5ml complete medium neutralization reaction, after piping and druming cell, proceeded in centrifuge tube, the centrifugal 5min of 1000rpm, adjusts 3 * 104/ml of concentration of cell suspension.
2) cell kind is entered in 96 well culture plates, every hole adds cell suspension 180 μ l, culture plate put into cell culture incubator (37 ℃, 5%CO2) cellar culture.
3) according to Growth of Cells situation, generally grow to 50%-70%, add cholagogic tablet solution, continue to cultivate 24h.
4) after 24h, add 20 μ l MTT solution (5mg/ml, i.e. 0.5%MTT), continue to cultivate 4h.
5) after 4h, buckle method is removed supernatant, with absorbent paper, pats dry gently, and every hole adds 200 μ l dimethyl sulfoxide, puts low-speed oscillation 10min on shaking table, and crystal is fully dissolved.At enzyme-linked immunosorbent assay instrument 490nm place, measure the light absorption value in each hole.
6) background (do not add cell, only add culture fluid) is set simultaneously, control wells (the medicine dissolution medium of cell, same concentrations, culture fluid, MTT, dimethyl sulfoxide), sets 6 multiple holes for every group.
7) result represents the suppression ratio of cell with medicine:
Cell increment suppression ratio (%)=(control wells OD value-dosing holes OD value)/control wells OD value * 100%.Experiment repeats 3 times.
3 statistical dispositions
Adopt correlation analysis and Student t check in Microsoft Excel2003 software, data represent with mean ± S.D..
4 experimental results
Statistical result showed after mtt assay experiment, with matched group comparison, when dosage reaches 5mg/ml, to B16 cell inhibitory effect variant (P<0.05), dosage this difference when 10mg/ml has significance (P<0.01), has utmost point significant difference (P<0.001) when dosage reaches 15-20mg/ml.
Table 1 cholagogic tablet is to S180 mice sarcoma cell propagation inhibitory effect
study carefully (X ± SD)
Note: with matched group comparison,
*p<0.01;
*p<0.001
5 experiment conclusion
Cholagogic tablet can suppress S180 mice sarcoma cell propagation, reduces the Growth of Cells number of S180 mice sarcoma cell, and this effect is dose dependent.
Claims (2)
1. the application of cholagogic tablet in suppressing S180 mice sarcoma cell propagation medicine, the preparation method that it is characterized in that cholagogic tablet is: get Radix Et Rhizoma Rhei 58g, Flos Lonicerae 58g, Herba Lysimachiae 58g, Radix Aucklandiae 96.5g, Rhizoma Anemarrhenae 58g, Folium Isatidis 58g, Radix Bupleuri 58g, Radix Paeoniae Alba 58g, Radix Scutellariae 29g, Natrii Sulfas 19g, Herba Artemisiae Scopariae 58g, above ten simply, Radix Et Rhizoma Rhei, Flos Lonicerae, Herba Lysimachiae, Radix Aucklandiae powder is broken into fine powder, the Rhizoma Anemarrhenae, Folium Isatidis, Radix Bupleuri, the Radix Paeoniae Alba, Radix Scutellariae, Herba Artemisiae Scopariae decocts with water twice, filter, merging filtrate, standing 12 hours, get supernatant standby, Natrii Sulfas adds 2 times of water, heating for dissolving, filter, filtrate adds in supernatant, mix, be condensed into thick paste shape, add above-mentioned fine powder to granulate, be pressed into 1000, sugar coating, obtain.
2. the application of a kind of cholagogic tablet in suppressing S180 mice sarcoma cell propagation medicine according to claim 1, it is characterized in that decocting with water twice, the 8-12 that adds for the first time water and be medical material weight doubly measures, and decocts 1-2h, the 6-10 that adds for the second time water and be medical material weight doubly measures, and decocts 1-2h.
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Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102397451A (en) * | 2011-11-26 | 2012-04-04 | 苏州派腾生物医药科技有限公司 | Preparation method of cholagogic tablet |
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102397451A (en) * | 2011-11-26 | 2012-04-04 | 苏州派腾生物医药科技有限公司 | Preparation method of cholagogic tablet |
Non-Patent Citations (4)
| Title |
|---|
| 国家药典委员会编: "《中华人民共和国药典：2010年版. 一部》", 31 January 2010, 中国医药科技出版社, article ""中华人民共和国药典"" * |
| 廖子君编著: "《现代肿瘤治疗药物学》", 28 February 2002, 世界图书出版西安公司, article ""现代肿瘤治疗药物学"" * |
| 杨建宇主编: "《抗癌中草药》", 31 October 2013, 化学工业出版社, article ""抗癌中草药"" * |
| 苗明三主编: "《中药药理学》", 31 March 2013, 湖南科学技术出版社, article ""湖南科学技术出版社"" * |
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Application publication date: 20140326 |