CN103655436B - A kind of biological fermentation facial cleanser and preparation method thereof - Google Patents
A kind of biological fermentation facial cleanser and preparation method thereof Download PDFInfo
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Abstract
The present invention relates to a kind of biological fermentation facial cleanser and preparation method thereof, the method comprises the following steps: poured in emulsification pot by whole water, be then slowly sprinkled into glycerine, is sprinkled into oligosaccharide malt glycosyl glucoside; AES is dropped in emulsification pot, stir and be warming up to 75 ~ 80 DEG C, be incubated half an hour after raw material all dissolves, add coconut oleoyl amine DEA and stir; Cool to less than 65 DEG C, add AMONYL 380LC, stir and to vacuumize and below slow cooling to 40 DEG C simultaneously; Add complex ferment amino acid successively and entirely imitate nutritive medium, extract solution from aloe, stir, stirring velocity 50 revs/min; Stirring is cooled to 38 DEG C, stops stirring, inspection discharging.Make biological fermentation facial cleanser.Compared with prior art, product performance of the present invention are gentle, and activeconstituents is high, and quality is also more moist, and have moisturizing, maintenance, whitening, anti-ageing, compact, nutrition moistens.
Description
Technical field
The present invention relates to a kind of washing and skin care articles for use, especially relate to a kind of biological fermentation facial cleanser and preparation method thereof.
Background technology
Substantially this all series products all adopts industrial chemicals to synthesize, though powerful, its side effect also clearly.This biological fermentation facial cleanser adopts biofermentation technique, comprises the whole nutrition required for skin, takes the course of its own at home.These product performance are gentle, and activeconstituents is high, and quality is also more moist, and have moisturizing, maintenance, whitening, anti-ageing, compact, nutrition moistens.
Summary of the invention
Object of the present invention is exactly provide a kind of performance gentle to overcome defect that above-mentioned prior art exists, activeconstituents is high, quality is also more moist, and have moisturizing, maintenance, whitening, anti-ageing, compact, biological fermentation facial cleanser and preparation method thereof that nutrition is moistened.
Object of the present invention can be achieved through the following technical solutions: a kind of biological fermentation facial cleanser, is characterized in that, comprises the component of following weight percentage:
Described complex ferment amino acid is entirely imitated nutritive medium and is obtained by the following method: raw material is placed in pottery static fermentation 21 ~ 30 days, then the product obtained is taken out and filter, collect filtrate and control temperature is 100 ~ 110 DEG C carries out high-temperature sterilization, finally be cooled to room temperature, namely obtain complex ferment amino acid and entirely imitate nutritive medium.
Described raw material comprises the component of following weight percent content: lactic acid bacterial liquid 0.5 ~ 1.0%, pink yeast liquid 0.5 ~ 1.0%, Phanerochaete chrysosporium bacterium liquid 0.5 ~ 1.0%, Bacillus licheniformis liquid 0.5 ~ 1.0%, bacillus pumilus bacterium liquid 0.5 ~ 1.0%, Candida maltosa bacterium liquid 0.5 ~ 1.0%, long shoot Trichoderma liquid 0.5 ~ 1.0%, shatian pomelo 0.4 ~ 0.6%, apple 0.4 ~ 0.6%, banana 0.4 ~ 0.6%, Lac regis apis 0.2 ~ 0.3%, extraordinary soya bean 1.0 ~ 1.2%, ginseng 0.5 ~ 0.9%, glossy ganoderma 0.5 ~ 0.9%, lavandula angustifolia 1.0 ~ 1.2%, rice distiller grain 20 ~ 25%, surplus is pure water.
Described lactic acid bacterial liquid prepares by the following method:
(1) prepare lactic acid bacteria culturing medium raw material, comprise following component and weight percent content: milk casein peptone 8 ~ 12%, peptone 8 ~ 12%, yeast extract paste 4 ~ 6%, glucose 4 ~ 6%, tween-80 0.8 ~ 1.2%, dipotassium hydrogen phosphate 1.8 ~ 2.5%, sodium-acetate 4 ~ 6%, citric acid diamines 1.8 ~ 2.5%, magnesium sulfate 0.1 ~ 0.3%, manganous sulfate 0.04 ~ 0.06%, surplus are pure water;
(2) preparation of liquid nutrient medium: by lactic acid bacteria culturing medium formulated raw material, at 0.15MPa, 121 DEG C, sterilizing 30min, obtains the liquid nutrient medium of sterilizing, is placed in fermentor tank;
(3) preparation of shaking flask bacterial classification: get the lactobacillus inoculation preserved on inclined-plane and access in sterilized liquid nutrient medium in gnotobasis, at 30 DEG C, rate of shaking 100 ~ 200 times/min, carries out shake-flask culture 48h, obtains shaking flask bacterium liquid;
(4) preparation of bacterium liquid is produced: by 3wt% ~ 4wt% inoculum size by shaking flask bacterium liquid access fermentor tank, at 30 DEG C, ventilating ratio is 1 ︰ 1 (V/V) liquid amount is ferment under the condition of 1/3, add the sterilized liquid nutrient medium continuation fermentation of 1/3 volume again by above-mentioned condition after 48h, stop fermentation after 96h, namely obtain lactic acid bacterial liquid.
Described pink yeast liquid prepares by the following method:
(1) prepare pink yeast culture based raw material, comprise following component and weight percent content: peptone 4.1 ~ 6%, glucose 8 ~ 12%, yeast extract paste 2 ~ 4%, surplus are wort;
(2) preparation of liquid nutrient medium: by pink yeast culture medium formulated raw material, at 0.15MPa, 121 DEG C, sterilizing 30min, obtains the liquid nutrient medium of sterilizing, is placed in fermentor tank;
(3) preparation of shaking flask bacterial classification: get the pink barms preserved on inclined-plane and access in sterilized liquid nutrient medium in gnotobasis, at 30 DEG C, rate of shaking 100 ~ 200 times/min, carries out shake-flask culture 48h, obtains shaking flask bacterium liquid;
(4) preparation of bacterium liquid is produced: by 3wt% ~ 4wt% inoculum size by shaking flask bacterium liquid access fermentor tank, at 30 DEG C, ventilating ratio is 1 ︰ 1 (V/V) liquid amount is ferment under the condition of 1/3, add the substratum continuation fermentation of 1/3 volume again by above-mentioned condition after 48h, stop fermentation after 96h, namely obtain pink yeast liquid.
Described Phanerochaete chrysosporium bacterium liquid prepares by the following method:
(1) prepare Phanerochaete chrysosporium culture medium raw material, comprise following component and weight percent content: glucose 1.9 ~ 2.0%, KH
2pO
40.25 ~ 0.30%, MgSO
47H
2o0.14 ~ 0.15%, vitaminB10 .01%, agar 1.4 ~ 1.5%, surplus are potato extracting solution;
(2) preparation of liquid nutrient medium: by Phanerochaete chrysosporium culture medium prescription preparation raw material, at 0.15MPa, 121 DEG C, sterilizing 30min, obtains the liquid nutrient medium of sterilizing, is placed in fermentor tank;
(3) preparation of shaking flask bacterial classification: get the Phanerochaete chrysosporium bacterial classification preserved on inclined-plane and access in sterilized liquid nutrient medium in gnotobasis, at 30 DEG C, rate of shaking 100 ~ 200 times/min, carries out shake-flask culture 48h, obtains shaking flask bacterium liquid;
(4) preparation of bacterium liquid is produced: by 3wt% ~ 4wt% inoculum size by shaking flask bacterium liquid access fermentor tank, at 30 DEG C, ventilating ratio is 1 ︰ 1 (V/V) liquid amount is ferment under the condition of 1/3, add the substratum continuation fermentation of 1/3 volume again by above-mentioned condition after 48h, stop fermentation after 96h, namely obtain Phanerochaete chrysosporium bacterium liquid.
Described Bacillus licheniformis liquid prepares by the following method:
(1) Bacillus licheniformis culture medium raw material comprises following component and weight percent content: wheat bran 4.5 ~ 4.7%, soybean cake powder 2.7 ~ 2.85%, agar 1.8 ~ 1.85%, surplus are the NaOH solution of 0.2wt%, after wheat bran, soybean cake powder mixing, add 0.2wt%NaOH solution and boil 1h, neutralizing pH with HCl is 7, then adds agar and melts;
(2) preparation of liquid nutrient medium: sample step (1) obtained is at 0.15MPa, and 121 DEG C, sterilizing 30min, obtains the liquid nutrient medium of sterilizing, be placed in fermentor tank;
(3) preparation of shaking flask bacterial classification: get the Bacillus licheniformis strain preserved on inclined-plane and access in sterilized liquid nutrient medium in gnotobasis, at 30 DEG C, rate of shaking 100 ~ 200 times/min, carries out shake-flask culture 48h, obtains shaking flask bacterium liquid;
(4) preparation of bacterium liquid is produced: by 3wt% ~ 4wt% inoculum size by shaking flask bacterium liquid access fermentor tank, at 30 DEG C, ventilating ratio is 1 ︰ 1 (V/V) liquid amount is ferment under the condition of 1/3, add the substratum continuation fermentation of 1/3 volume again by above-mentioned condition after 48h, stop fermentation after 96h, namely obtain Bacillus licheniformis liquid.
Described bacillus pumilus bacterium liquid prepares by the following method:
(1) prepare bacillus pumilus culture medium raw material, comprise following component and weight percent content: peptone 0.4 ~ 0.6%, beef leaching thing 0.25 ~ 0.35%, NaCl0.4 ~ 0.6%, agar 1.4 ~ 1.5%, surplus are distilled water;
(2) preparation of liquid nutrient medium: by bacillus pumilus culture medium prescription preparation raw material, at 0.15MPa, 121 DEG C, sterilizing 30min, obtains the liquid nutrient medium of sterilizing, is placed in fermentor tank;
(3) preparation of shaking flask bacterial classification: get the bacillus pumilus bacterial classification preserved on inclined-plane and access in sterilized liquid nutrient medium in gnotobasis, at 30 DEG C, rate of shaking 100 ~ 200 times/min, carries out shake-flask culture 48h, obtains shaking flask bacterium liquid;
(4) preparation of bacterium liquid is produced: by 3wt% ~ 4wt% inoculum size by shaking flask bacterium liquid access fermentor tank, at 30 DEG C, ventilating ratio is 1 ︰ 1 (V/V) liquid amount is ferment under the condition of 1/3, add the substratum continuation fermentation of 1/3 volume again by above-mentioned condition after 48h, stop fermentation after 96h, namely obtain bacillus pumilus bacterium liquid.
Described Candida maltosa bacterium liquid prepares by the following method:
(1) prepare Candida maltosa culture medium raw material, comprise following component and weight percent content: agar 1.4 ~ 1.6%; 5 ° of worts 98.4 ~ 98.6%;
(2) preparation of liquid nutrient medium: by Candida maltosa culture medium prescription preparation raw material, at 0.15MPa, 121 DEG C, sterilizing 30min, obtains the liquid nutrient medium of sterilizing, is placed in fermentor tank;
(3) preparation of shaking flask bacterial classification: get the Candida maltosa bacterial classification preserved on inclined-plane and access in sterilized liquid nutrient medium in gnotobasis, at 30 DEG C, rate of shaking 100 ~ 200 times/min, carries out shake-flask culture 48h, obtains shaking flask bacterium liquid;
(4) preparation of bacterium liquid is produced: by 3wt% ~ 4wt% inoculum size by shaking flask bacterium liquid access fermentor tank, at 30 DEG C, ventilating ratio is 1 ︰ 1 (V/V) liquid amount is ferment under the condition of 1/3, add the substratum continuation fermentation of 1/3 volume again by above-mentioned condition after 48h, stop fermentation after 96h, namely obtain Candida maltosa bacterium liquid;
Described long shoot Trichoderma liquid prepares by the following method:
(1) prepare the mould culture medium raw material of long shoot wood, comprise following component and weight percent content: agar 1.4 ~ 1.6%; 5 ° of worts 98.4 ~ 98.6%;
(2) preparation of liquid nutrient medium: by the mould culture medium prescription preparation raw material of long shoot wood, at 0.15MPa, 121 DEG C, sterilizing 30min, obtains the liquid nutrient medium of sterilizing, be placed in fermentor tank;
(3) preparation of shaking flask bacterial classification: get the long shoot Trichoderma kind of preserving on inclined-plane and access in sterilized liquid nutrient medium in gnotobasis, at 30 DEG C, rate of shaking 100 ~ 200 times/min, carries out shake-flask culture 48h, obtains shaking flask bacterium liquid;
(4) preparation of bacterium liquid is produced: by 3wt% ~ 4wt% inoculum size by shaking flask bacterium liquid access fermentor tank, at 30 DEG C, ventilating ratio is 1 ︰ 1 (V/V) liquid amount is ferment under the condition of 1/3, add the substratum continuation fermentation of 1/3 volume again by above-mentioned condition after 48h, stop fermentation after 96h, namely obtain long shoot Trichoderma liquid;
Described apple is the apple selecting the Qinling Mountains or Dalian,
The banana in Yunnan selected by described banana,
Described extraordinary soya bean selects the extraordinary soya bean into Shaanxi or Heilungkiang.
A preparation method for biological fermentation facial cleanser, is characterized in that, the method comprises the following steps:
(1) take by weight percentage: complex ferment amino acid imitates nutritive medium 10% ~ 15% entirely; AES15% ~ 17%; Oligosaccharide malt glycosyl glucoside 2% ~ 3%; Coconut oleoyl amine DEA3% ~ 4%; Glycerine 2.5% ~ 3.5%; AMONYL 380LC 6% ~ 8%; Extract solution from aloe 1.8% ~ 2.2%; Water surplus;
(2) poured in emulsification pot by whole water, be then slowly sprinkled into glycerine, stir after within 15 minutes, being uniformly dispersed, be sprinkled into oligosaccharide malt glycosyl glucoside, stir and be uniformly dispersed for 15 minutes, stirring velocity is 80 revs/min;
(3) dropped into by AES in emulsification pot, dispersed with stirring is even, and heat simultaneously, be warming up to 75 ~ 80 DEG C, be incubated half an hour after raw material all dissolves, stirring velocity is 50 revs/min;
(4) add coconut oleoyl amine DEA to stir and be uniformly dissolved for 15 minutes, stirring velocity is 50 revs/min;
(5) cool to less than 65 DEG C, add AMONYL 380LC, stir, to vacuumize and below slow cooling to 40 DEG C simultaneously, stirring velocity 50 revs/min, each raw material adds rear stirring 10-15 minute, adds a rear raw material again after guarantee stirs;
(6) add complex ferment amino acid successively and entirely imitate nutritive medium, extract solution from aloe, stir, stirring velocity 50 revs/min, each raw material adds rear stirring 10-15 minute, adds a rear raw material again after guarantee stirs;
(7) stirring is cooled to 38 DEG C, stops stirring, inspection discharging.Make biological fermentation facial cleanser.
The main component of biological fermentation facial cleanser of the present invention is that complex ferment amino acid imitates nutritive medium entirely, it is rich in necessary 18 seed amino acids of skin cell, soybean lsoflavons, oligose, the nutritive substance of more than the 40 kind of needed by human such as mineral substance and trace element, β-carotene, VitB1, citric acid such as soybean phospholipid, soybean saponin, VitC, VitE, B group VITAMIN, zinc, selenium, phosphorus, iron, calcium.
The complex ferment amino acid that described biological fermentation facial cleanser adopts entirely is imitated nutritive medium, essence etc. and is adopted natural biology extract, does not add hormonal substance.
Wherein:
AES (ethoxylated dodecyl alcohol sodium sulfate) and coconut oleoyl amine DEA combinationally uses good water solubility, wash protect effective, strong detergency, foaming abundancy.
Oligosaccharide malt glycosyl glucoside is the carbohydrate syrup born by starch derivatives, and containing various carbohydrate, can protect skin cells and moisturizing, product is fine quality, to skin without any stimulation.
AMONYL 380LC has excellent foaminess and significant thickening property, strong to fungi restraint, effective.
Glycerine is pleasantly sweet, and be water white transparency viscous liquid, moisturizing effect is very good, with the soft skin of rear water water profit profit.
Extract solution from aloe is that natural moisturizing promotes that NMF falls in fibrous polymer albumen with lock water, effect mechanism, thus increases natural moisturizing factor, immediately increases water-content, is reduced through skin water loss by slight filming function.
Compared with prior art, biological fermentation facial cleanser of the present invention adopts biofermentation technique, comprises the whole nutrition required for hair quality, takes the course of its own at home.These product performance are gentle, and activeconstituents is high, and quality is also more moist, and have moisturizing, maintenance, whitening, anti-ageing, compact, nutrition moistens.
Embodiment
Below in conjunction with specific embodiment, the present invention is described in detail.
Embodiment 1
A preparation method for biological fermentation facial cleanser, the method comprises the following steps:
(1) take by weight percentage: complex ferment amino acid imitates nutritive medium 12% entirely; AES16%; Oligosaccharide malt glycosyl glucoside 2.5%; Coconut oleoyl amine DEA3.5%; Glycerine 3%; AMONYL 380LC 7%; Extract solution from aloe 2%; Water surplus;
(2) poured in emulsification pot by whole water, be then slowly sprinkled into glycerine, stir after within 15 minutes, being uniformly dispersed, be sprinkled into oligosaccharide malt glycosyl glucoside, stir and be uniformly dispersed for 15 minutes, stirring velocity is 80 revs/min;
(3) dropped into by AES in emulsification pot, dispersed with stirring is even, and heat simultaneously, be warming up to 75 ~ 80 DEG C, be incubated half an hour after raw material all dissolves, stirring velocity is 50 revs/min;
(4) add coconut oleoyl amine DEA to stir and be uniformly dissolved for 15 minutes, stirring velocity is 50 revs/min;
(5) cool to less than 65 DEG C, add AMONYL 380LC, stir, to vacuumize and below slow cooling to 40 DEG C simultaneously, stirring velocity 50 revs/min, each raw material adds rear stirring 10-15 minute, adds a rear raw material again after guarantee stirs;
(6) add complex ferment amino acid successively and entirely imitate nutritive medium, extract solution from aloe, stir, stirring velocity 50 revs/min, each raw material adds rear stirring 10-15 minute, adds a rear raw material again after guarantee stirs;
(7) stirring is cooled to 38 DEG C, stops stirring, inspection discharging.Make biological fermentation facial cleanser.
The main component of biological fermentation facial cleanser of the present invention is that complex ferment amino acid imitates nutritive medium entirely, it is rich in necessary 18 seed amino acids of skin cell, soybean lsoflavons, oligose, the nutritive substance of more than the 40 kind of needed by human such as mineral substance and trace element, β-carotene, VitB1, citric acid such as soybean phospholipid, soybean saponin, VitC, VitE, B group VITAMIN, zinc, selenium, phosphorus, iron, calcium.
Above-mentioned complex ferment amino acid imitates nutritive medium entirely, raw material comprises following component and weight percent content: lactic acid bacterial liquid 0.5%, pink yeast liquid 0.5%, Phanerochaete chrysosporium bacterium liquid 0.5%, Bacillus licheniformis liquid 0.5%, bacillus pumilus bacterium liquid 0.5%, Candida maltosa bacterium liquid 0.5%, long shoot Trichoderma liquid 0.5%, shatian pomelo 0.4%, the place of production is the apple 0.4% in the Qinling Mountains, the place of production is the banana 0.4% in Yunnan, Lac regis apis 0.2%, the place of production is the extraordinary soya bean 1.0% in Shaanxi, ginseng 0.5%, glossy ganoderma 0.5%, lavandula angustifolia 1.0%, rice distiller grain 20%, surplus is pure water.
Lactic acid bacterial liquid prepares by the following method:
(1) prepare lactic acid bacteria culturing medium raw material, comprise following component and weight percent content: milk casein peptone 8%, peptone 8%, yeast extract paste 4%, glucose 4%, tween-80 0.8%, dipotassium hydrogen phosphate 1.8%, sodium-acetate 4%, citric acid diamines 1.8%, magnesium sulfate 0.1%, manganous sulfate 0.04%, surplus are pure water;
(2) preparation of liquid nutrient medium: by lactic acid bacteria culturing medium formulated raw material, at 0.15MPa, 121 DEG C, sterilizing 30min, obtains the liquid nutrient medium of sterilizing, is placed in fermentor tank;
(3) preparation of shaking flask bacterial classification: get the lactobacillus inoculation preserved on inclined-plane and access in sterilized liquid nutrient medium in gnotobasis, at 30 DEG C, rate of shaking 100 times/min, carries out shake-flask culture 48h, obtains shaking flask bacterium liquid;
(4) preparation of bacterium liquid is produced: by 3wt% inoculum size by shaking flask bacterium liquid access fermentor tank, at 30 DEG C, ventilating ratio is 1 ︰ 1 (V/V) liquid amount is ferment under the condition of 1/3, add the sterilized liquid nutrient medium continuation fermentation of 1/3 volume again by above-mentioned condition after 48h, stop fermentation after 96h, namely obtain lactic acid bacterial liquid.
Pink yeast liquid prepares by the following method:
(1) prepare pink yeast culture based raw material, comprise following component and weight percent content: peptone 4.1%, glucose 8%, yeast extract paste 2%, surplus are wort;
(2) preparation of liquid nutrient medium: by pink yeast culture medium formulated raw material, at 0.15MPa, 121 DEG C, sterilizing 30min, obtains the liquid nutrient medium of sterilizing, is placed in fermentor tank;
(3) preparation of shaking flask bacterial classification: get the pink barms preserved on inclined-plane and access in sterilized liquid nutrient medium in gnotobasis, at 30 DEG C, rate of shaking 100 times/min, carries out shake-flask culture 48h, obtains shaking flask bacterium liquid;
(4) preparation of bacterium liquid is produced: by 3wt% inoculum size by shaking flask bacterium liquid access fermentor tank, at 30 DEG C, ventilating ratio is 1 ︰ 1 (V/V) liquid amount is ferment under the condition of 1/3, add the substratum continuation fermentation of 1/3 volume again by above-mentioned condition after 48h, stop fermentation after 96h, namely obtain pink yeast liquid.
Phanerochaete chrysosporium bacterium liquid prepares by the following method:
(1) prepare Phanerochaete chrysosporium culture medium raw material, comprise following component and weight percent content: glucose 1.9%, KH
2pO
40.25%, MgSO
47H
2o0.14%, vitaminB10 .01%, agar 1.4%, surplus are potato extracting solution;
(2) preparation of liquid nutrient medium: by Phanerochaete chrysosporium culture medium prescription preparation raw material, at 0.15MPa, 121 DEG C, sterilizing 30min, obtains the liquid nutrient medium of sterilizing, is placed in fermentor tank;
(3) preparation of shaking flask bacterial classification: get the Phanerochaete chrysosporium bacterial classification preserved on inclined-plane and access in sterilized liquid nutrient medium in gnotobasis, at 30 DEG C, rate of shaking 100 times/min, carries out shake-flask culture 48h, obtains shaking flask bacterium liquid;
(4) preparation of bacterium liquid is produced: by 3wt% inoculum size by shaking flask bacterium liquid access fermentor tank, at 30 DEG C, ventilating ratio is 1 ︰ 1 (V/V) liquid amount is ferment under the condition of 1/3, add the substratum continuation fermentation of 1/3 volume again by above-mentioned condition after 48h, stop fermentation after 96h, namely obtain Phanerochaete chrysosporium bacterium liquid.
Bacillus licheniformis liquid prepares by the following method:
(1) Bacillus licheniformis culture medium raw material comprises following component and weight percent content: wheat bran 4.5%, soybean cake powder 2.7%, agar 1.8%, surplus are the NaOH solution of 0.2wt%, after wheat bran, soybean cake powder mixing, add 0.2wt%NaOH solution and boil 1h, neutralizing pH with HCl is 7, then adds agar and melts;
(2) preparation of liquid nutrient medium: sample step (1) obtained is at 0.15MPa, and 121 DEG C, sterilizing 30min, obtains the liquid nutrient medium of sterilizing, be placed in fermentor tank;
(3) preparation of shaking flask bacterial classification: get the Bacillus licheniformis strain preserved on inclined-plane and access in sterilized liquid nutrient medium in gnotobasis, at 30 DEG C, rate of shaking 100 times/min, carries out shake-flask culture 48h, obtains shaking flask bacterium liquid;
(4) preparation of bacterium liquid is produced: by 3wt% inoculum size by shaking flask bacterium liquid access fermentor tank, at 30 DEG C, ventilating ratio is 1 ︰ 1 (V/V) liquid amount is ferment under the condition of 1/3, add the substratum continuation fermentation of 1/3 volume again by above-mentioned condition after 48h, stop fermentation after 96h, namely obtain Bacillus licheniformis liquid.
Bacillus pumilus bacterium liquid prepares by the following method:
(1) prepare bacillus pumilus culture medium raw material, comprise following component and weight percent content: peptone 0.4%, beef leaching thing 0.25%, NaCl0.4%, agar 1.4%, surplus are distilled water;
(2) preparation of liquid nutrient medium: by bacillus pumilus culture medium prescription preparation raw material, at 0.15MPa, 121 DEG C, sterilizing 30min, obtains the liquid nutrient medium of sterilizing, is placed in fermentor tank;
(3) preparation of shaking flask bacterial classification: get the bacillus pumilus bacterial classification preserved on inclined-plane and access in sterilized liquid nutrient medium in gnotobasis, at 30 DEG C, rate of shaking 100 times/min, carries out shake-flask culture 48h, obtains shaking flask bacterium liquid;
(4) preparation of bacterium liquid is produced: by 3wt% inoculum size by shaking flask bacterium liquid access fermentor tank, at 30 DEG C, ventilating ratio is 1 ︰ 1 (V/V) liquid amount is ferment under the condition of 1/3, add the substratum continuation fermentation of 1/3 volume again by above-mentioned condition after 48h, stop fermentation after 96h, namely obtain bacillus pumilus bacterium liquid.
Candida maltosa bacterium liquid prepares by the following method:
(1) prepare Candida maltosa culture medium raw material, comprise following component and weight percent content: agar 1.4%; 5 ° of worts 98.6%;
(2) preparation of liquid nutrient medium: by Candida maltosa culture medium prescription preparation raw material, at 0.15MPa, 121 DEG C, sterilizing 30min, obtains the liquid nutrient medium of sterilizing, is placed in fermentor tank;
(3) preparation of shaking flask bacterial classification: get the Candida maltosa bacterial classification preserved on inclined-plane and access in sterilized liquid nutrient medium in gnotobasis, at 30 DEG C, rate of shaking 100 times/min, carries out shake-flask culture 48h, obtains shaking flask bacterium liquid;
(4) preparation of bacterium liquid is produced: by 3wt% inoculum size by shaking flask bacterium liquid access fermentor tank, at 30 DEG C, ventilating ratio is 1 ︰ 1 (V/V) liquid amount is ferment under the condition of 1/3, add the substratum continuation fermentation of 1/3 volume again by above-mentioned condition after 48h, stop fermentation after 96h, namely obtain Candida maltosa bacterium liquid.
Long shoot Trichoderma liquid prepares by the following method:
(1) prepare the mould culture medium raw material of long shoot wood, comprise following component and weight percent content: agar 1.4%; 5 ° of worts 98.6%;
(2) preparation of liquid nutrient medium: by the mould culture medium prescription preparation raw material of long shoot wood, at 0.15MPa, 121 DEG C, sterilizing 30min, obtains the liquid nutrient medium of sterilizing, be placed in fermentor tank;
(3) preparation of shaking flask bacterial classification: get the long shoot Trichoderma kind of preserving on inclined-plane and access in sterilized liquid nutrient medium in gnotobasis, at 30 DEG C, rate of shaking 100 times/min, carries out shake-flask culture 48h, obtains shaking flask bacterium liquid;
(4) preparation of bacterium liquid is produced: by 3wt% inoculum size by shaking flask bacterium liquid access fermentor tank, at 30 DEG C, ventilating ratio is 1 ︰ 1 (V/V) liquid amount is ferment under the condition of 1/3, add the substratum continuation fermentation of 1/3 volume again by above-mentioned condition after 48h, stop fermentation after 96h, namely obtain long shoot Trichoderma liquid.
Complex ferment amino acid imitates nutritive medium in the preparation entirely, after preparing raw material according to formula, raw material pulverizing is puddled evenly, be placed in pottery static fermentation 30 days, then the product obtained is taken out and filter, collect filtrate and control temperature is 110 DEG C carries out high-temperature sterilization, be finally cooled to room temperature, namely obtain complex ferment amino acid and entirely imitate nutritive medium.
Embodiment 2
A preparation method for biological fermentation facial cleanser, the method comprises the following steps:
(1) take by weight percentage: complex ferment amino acid imitates nutritive medium 15% entirely; AES17%; Oligosaccharide malt glycosyl glucoside 3%; Coconut oleoyl amine DEA4%; Glycerine 3.5%; AMONYL 380LC 8%; Extract solution from aloe 2.2%; Water surplus;
(2) poured in emulsification pot by whole water, be then slowly sprinkled into glycerine, stir after within 15 minutes, being uniformly dispersed, be sprinkled into oligosaccharide malt glycosyl glucoside, stir and be uniformly dispersed for 15 minutes, stirring velocity is 80 revs/min;
(3) dropped into by AES in emulsification pot, dispersed with stirring is even, and heat simultaneously, be warming up to 75 ~ 80 DEG C, be incubated half an hour after raw material all dissolves, stirring velocity is 50 revs/min;
(4) add coconut oleoyl amine DEA to stir and be uniformly dissolved for 15 minutes, stirring velocity is 50 revs/min;
(5) cool to less than 65 DEG C, add AMONYL 380LC, stir, to vacuumize and below slow cooling to 40 DEG C simultaneously, stirring velocity 50 revs/min, each raw material adds rear stirring 10-15 minute, adds a rear raw material again after guarantee stirs;
(6) add complex ferment amino acid successively and entirely imitate nutritive medium, extract solution from aloe, stir, stirring velocity 50 revs/min, each raw material adds rear stirring 10-15 minute, adds a rear raw material again after guarantee stirs;
(7) stirring is cooled to 38 DEG C, stops stirring, inspection discharging.Make biological fermentation facial cleanser.
The main component of biological fermentation facial cleanser of the present invention is that complex ferment amino acid imitates nutritive medium entirely, it is rich in necessary 18 seed amino acids of skin cell, soybean lsoflavons, oligose, the nutritive substance of more than the 40 kind of needed by human such as mineral substance and trace element, β-carotene, VitB1, citric acid such as soybean phospholipid, soybean saponin, VitC, VitE, B group VITAMIN, zinc, selenium, phosphorus, iron, calcium.
Above-mentioned complex ferment amino acid imitates nutritive medium entirely, comprise following component and weight percent content: lactic acid bacterial liquid 0.6%, pink yeast liquid 0.8%, Phanerochaete chrysosporium bacterium liquid 0.8%, Bacillus licheniformis liquid 0.6%, bacillus pumilus bacterium liquid 0.7%, Candida maltosa bacterium liquid 0.7%, long shoot Trichoderma liquid 0.8%, shatian pomelo 0.5%, the apple 0.5% in Dalian, the place of production, the banana 0.5% in Yunnan, the place of production, Lac regis apis 0.2%, the extraordinary soya bean 1.1% in Heilungkiang, the place of production, ginseng 0.6%, glossy ganoderma 0.6%, lavandula angustifolia 1.1%, rice distiller grain 22%, surplus is pure water.
Lactic acid bacterial liquid prepares by the following method:
(1) prepare lactic acid bacteria culturing medium raw material, comprise following component and weight percent content: milk casein peptone 10%, peptone 10%, yeast extract paste 5%, glucose 5%, tween-80 1%, dipotassium hydrogen phosphate 2%, sodium-acetate 5%, citric acid diamines 2%, magnesium sulfate 0.2%, manganous sulfate 0.05%, surplus are pure water;
(2) preparation of liquid nutrient medium: by lactic acid bacteria culturing medium formulated raw material, at 0.15MPa, 121 DEG C, sterilizing 30min, obtains the liquid nutrient medium of sterilizing, is placed in fermentor tank;
(3) preparation of shaking flask bacterial classification: get the lactobacillus inoculation preserved on inclined-plane and access in sterilized liquid nutrient medium in gnotobasis, at 30 DEG C, rate of shaking 200 times/min, carries out shake-flask culture 48h, obtains shaking flask bacterium liquid;
(4) preparation of bacterium liquid is produced: by 3wt% inoculum size by shaking flask bacterium liquid access fermentor tank, at 30 DEG C, ventilating ratio is 1 ︰ 1 (V/V) liquid amount is ferment under the condition of 1/3, add the sterilized liquid nutrient medium continuation fermentation of 1/3 volume again by above-mentioned condition after 48h, stop fermentation after 96h, namely obtain lactic acid bacterial liquid.
Pink yeast liquid prepares by the following method:
(1) prepare pink yeast culture based raw material, comprise following component and weight percent content: peptone 5%, glucose 10%, yeast extract paste 3%, surplus are wort;
(2) preparation of liquid nutrient medium: by pink yeast culture medium formulated raw material, at 0.15MPa, 121 DEG C, sterilizing 30min, obtains the liquid nutrient medium of sterilizing, is placed in fermentor tank;
(3) preparation of shaking flask bacterial classification: get the pink barms preserved on inclined-plane and access in sterilized liquid nutrient medium in gnotobasis, at 30 DEG C, rate of shaking 100 times/min, carries out shake-flask culture 48h, obtains shaking flask bacterium liquid;
(4) preparation of bacterium liquid is produced: by 3wt% inoculum size by shaking flask bacterium liquid access fermentor tank, at 30 DEG C, ventilating ratio is 1 ︰ 1 (V/V) liquid amount is ferment under the condition of 1/3, add the substratum continuation fermentation of 1/3 volume again by above-mentioned condition after 48h, stop fermentation after 96h, namely obtain pink yeast liquid.
Phanerochaete chrysosporium bacterium liquid prepares by the following method:
(1) prepare Phanerochaete chrysosporium culture medium raw material, comprise following component and weight percent content: glucose 1.9%, KH
2pO
40.28%, MgSO
47H
2o0.14%, vitaminB10 .01%, agar 1.4%, surplus are potato extracting solution;
(2) preparation of liquid nutrient medium: by Phanerochaete chrysosporium culture medium prescription preparation raw material, at 0.15MPa, 121 DEG C, sterilizing 30min, obtains the liquid nutrient medium of sterilizing, is placed in fermentor tank;
(3) preparation of shaking flask bacterial classification: get the Phanerochaete chrysosporium bacterial classification preserved on inclined-plane and access in sterilized liquid nutrient medium in gnotobasis, at 30 DEG C, rate of shaking 100 times/min, carries out shake-flask culture 48h, obtains shaking flask bacterium liquid;
(4) preparation of bacterium liquid is produced: by 3wt% inoculum size by shaking flask bacterium liquid access fermentor tank, at 30 DEG C, ventilating ratio is 1 ︰ 1 (V/V) liquid amount is ferment under the condition of 1/3, add the substratum continuation fermentation of 1/3 volume again by above-mentioned condition after 48h, stop fermentation after 96h, namely obtain Phanerochaete chrysosporium bacterium liquid.
Bacillus licheniformis liquid prepares by the following method:
(1) Bacillus licheniformis culture medium raw material comprises following component and weight percent content: wheat bran 4.6%, soybean cake powder 2.8%, agar 1.8%, surplus are the NaOH solution of 0.2wt%, after wheat bran, soybean cake powder mixing, add 0.2wt%NaOH solution and boil 1h, neutralizing pH with HCl is 7, then adds agar and melts;
(2) preparation of liquid nutrient medium: sample step (1) obtained is at 0.15MPa, and 121 DEG C, sterilizing 30min, obtains the liquid nutrient medium of sterilizing, be placed in fermentor tank;
(3) preparation of shaking flask bacterial classification: get the Bacillus licheniformis strain preserved on inclined-plane and access in sterilized liquid nutrient medium in gnotobasis, at 30 DEG C, rate of shaking 100 times/min, carries out shake-flask culture 48h, obtains shaking flask bacterium liquid;
(4) preparation of bacterium liquid is produced: by 3wt%% inoculum size by shaking flask bacterium liquid access fermentor tank, at 30 DEG C, ventilating ratio is 1 ︰ 1 (V/V) liquid amount is ferment under the condition of 1/3, add the substratum continuation fermentation of 1/3 volume again by above-mentioned condition after 48h, stop fermentation after 96h, namely obtain Bacillus licheniformis liquid.
Bacillus pumilus bacterium liquid prepares by the following method:
(1) prepare bacillus pumilus culture medium raw material, comprise following component and weight percent content: peptone 0.5%, beef leaching thing 0.3%, NaCl0.5%, agar 1.4%, surplus are distilled water;
(2) preparation of liquid nutrient medium: by bacillus pumilus culture medium prescription preparation raw material, at 0.15MPa, 121 DEG C, sterilizing 30min, obtains the liquid nutrient medium of sterilizing, is placed in fermentor tank;
(3) preparation of shaking flask bacterial classification: get the bacillus pumilus bacterial classification preserved on inclined-plane and access in sterilized liquid nutrient medium in gnotobasis, at 30 DEG C, rate of shaking 100 times/min, carries out shake-flask culture 48h, obtains shaking flask bacterium liquid;
(4) preparation of bacterium liquid is produced: by 4wt% inoculum size by shaking flask bacterium liquid access fermentor tank, at 30 DEG C, ventilating ratio is 1 ︰ 1 (V/V) liquid amount is ferment under the condition of 1/3, add the substratum continuation fermentation of 1/3 volume again by above-mentioned condition after 48h, stop fermentation after 96h, namely obtain bacillus pumilus bacterium liquid.
Candida maltosa bacterium liquid prepares by the following method:
(1) prepare Candida maltosa culture medium raw material, comprise following component and weight percent content: agar 1.5%; 5 ° of worts 98.5%;
(2) preparation of liquid nutrient medium: by Candida maltosa culture medium prescription preparation raw material, at 0.15MPa, 121 DEG C, sterilizing 30min, obtains the liquid nutrient medium of sterilizing, is placed in fermentor tank;
(3) preparation of shaking flask bacterial classification: get the Candida maltosa bacterial classification preserved on inclined-plane and access in sterilized liquid nutrient medium in gnotobasis, at 30 DEG C, rate of shaking 100 times/min, carries out shake-flask culture 48h, obtains shaking flask bacterium liquid;
(4) preparation of bacterium liquid is produced: by 3wt% inoculum size by shaking flask bacterium liquid access fermentor tank, at 30 DEG C, ventilating ratio is 1 ︰ 1 (V/V) liquid amount is ferment under the condition of 1/3, add the substratum continuation fermentation of 1/3 volume again by above-mentioned condition after 48h, stop fermentation after 96h, namely obtain Candida maltosa bacterium liquid.
Long shoot Trichoderma liquid prepares by the following method:
(1) prepare the mould culture medium raw material of long shoot wood, comprise following component and weight percent content: agar 1.5%; 5 ° of worts 98.5%;
(2) preparation of liquid nutrient medium: by the mould culture medium prescription preparation raw material of long shoot wood, at 0.15MPa, 121 DEG C, sterilizing 30min, obtains the liquid nutrient medium of sterilizing, be placed in fermentor tank;
(3) preparation of shaking flask bacterial classification: get the long shoot Trichoderma kind of preserving on inclined-plane and access in sterilized liquid nutrient medium in gnotobasis, at 30 DEG C, rate of shaking 100 times/min, carries out shake-flask culture 48h, obtains shaking flask bacterium liquid;
(4) preparation of bacterium liquid is produced: by 3wt% inoculum size by shaking flask bacterium liquid access fermentor tank, at 30 DEG C, ventilating ratio is 1 ︰ 1 (V/V) liquid amount is ferment under the condition of 1/3, add the substratum continuation fermentation of 1/3 volume again by above-mentioned condition after 48h, stop fermentation after 96h, namely obtain long shoot Trichoderma liquid.
Complex ferment amino acid imitates nutritive medium in the preparation entirely, after preparing raw material according to formula, raw material pulverizing is puddled evenly, be placed in pottery static fermentation 30 days, then the product obtained is taken out and filter, collect filtrate and control temperature is 110 DEG C carries out high-temperature sterilization, be finally cooled to room temperature, namely obtain complex ferment amino acid and entirely imitate nutritive medium.
Embodiment 3
A preparation method for biological fermentation facial cleanser, the method comprises the following steps:
(1) take by weight percentage: complex ferment amino acid imitates nutritive medium 10% entirely; AES15%; Oligosaccharide malt glycosyl glucoside 2%; Coconut oleoyl amine DEA3%; Glycerine 2.5%; AMONYL 380LC 6%; Extract solution from aloe 1.8%; Water surplus;
(2) poured in emulsification pot by whole water, be then slowly sprinkled into glycerine, stir after within 15 minutes, being uniformly dispersed, be sprinkled into oligosaccharide malt glycosyl glucoside, stir and be uniformly dispersed for 15 minutes, stirring velocity is 80 revs/min;
(3) dropped into by AES in emulsification pot, dispersed with stirring is even, and heat simultaneously, be warming up to 75 ~ 80 DEG C, be incubated half an hour after raw material all dissolves, stirring velocity is 50 revs/min;
(4) add coconut oleoyl amine DEA to stir and be uniformly dissolved for 15 minutes, stirring velocity is 50 revs/min;
(5) cool to less than 65 DEG C, add AMONYL 380LC, stir, to vacuumize and below slow cooling to 40 DEG C simultaneously, stirring velocity 50 revs/min, each raw material adds rear stirring 10-15 minute, adds a rear raw material again after guarantee stirs;
(6) add complex ferment amino acid successively and entirely imitate nutritive medium, extract solution from aloe, stir, stirring velocity 50 revs/min, each raw material adds rear stirring 10-15 minute, adds a rear raw material again after guarantee stirs;
(7) stirring is cooled to 38 DEG C, stops stirring, inspection discharging.Make biological fermentation facial cleanser.
The main component of biological fermentation facial cleanser of the present invention is that complex ferment amino acid imitates nutritive medium entirely, it is rich in necessary 18 seed amino acids of skin cell, soybean lsoflavons, oligose, the nutritive substance of more than the 40 kind of needed by human such as mineral substance and trace element, β-carotene, VitB1, citric acid such as soybean phospholipid, soybean saponin, VitC, VitE, B group VITAMIN, zinc, selenium, phosphorus, iron, calcium.
Above-mentioned complex ferment amino acid imitates nutritive medium entirely, comprise following component and weight percent content: lactic acid bacterial liquid 1.0%, pink yeast liquid 1.0%, Phanerochaete chrysosporium bacterium liquid 1.0%, Bacillus licheniformis liquid 1.0%, bacillus pumilus bacterium liquid 1.0%, Candida maltosa bacterium liquid 1.0%, long shoot Trichoderma liquid 1.0%, shatian pomelo 0.6%, the apple 0.6% in Dalian, the place of production, the banana 0.6% in Yunnan, the place of production, Lac regis apis 0.3%, the extraordinary soya bean 1.2% in Heilungkiang, the place of production, ginseng 0.9%, glossy ganoderma 0.9%, lavandula angustifolia 1.2%, rice distiller grain 25%, surplus is pure water.
Lactic acid bacterial liquid prepares by the following method:
(1) prepare lactic acid bacteria culturing medium raw material, comprise following component and weight percent content: milk casein peptone 12%, peptone 12%, yeast extract paste 6%, glucose 6%, tween-80 1.2%, dipotassium hydrogen phosphate 2.5%, sodium-acetate 6%, citric acid diamines 2.5%, magnesium sulfate 0.3%, manganous sulfate 0.06%, surplus are pure water;
(2) preparation of liquid nutrient medium: by lactic acid bacteria culturing medium formulated raw material, at 0.15MPa, 121 DEG C, sterilizing 30min, obtains the liquid nutrient medium of sterilizing, is placed in fermentor tank;
(3) preparation of shaking flask bacterial classification: get the lactobacillus inoculation preserved on inclined-plane and access in sterilized liquid nutrient medium in gnotobasis, at 30 DEG C, rate of shaking 200 times/min, carries out shake-flask culture 48h, obtains shaking flask bacterium liquid;
(4) preparation of bacterium liquid is produced: by 4wt% inoculum size by shaking flask bacterium liquid access fermentor tank, at 30 DEG C, ventilating ratio is 1 ︰ 1 (V/V) liquid amount is ferment under the condition of 1/3, add the sterilized liquid nutrient medium continuation fermentation of 1/3 volume again by above-mentioned condition after 48h, stop fermentation after 96h, namely obtain lactic acid bacterial liquid.
Pink yeast liquid prepares by the following method:
(1) prepare pink yeast culture based raw material, comprise following component and weight percent content: peptone 6%, glucose 12%, yeast extract paste 4%, surplus are wort;
(2) preparation of liquid nutrient medium: by pink yeast culture medium formulated raw material, at 0.15MPa, 121 DEG C, sterilizing 30min, obtains the liquid nutrient medium of sterilizing, is placed in fermentor tank;
(3) preparation of shaking flask bacterial classification: get the pink barms preserved on inclined-plane and access in sterilized liquid nutrient medium in gnotobasis, at 30 DEG C, rate of shaking 200 times/min, carries out shake-flask culture 48h, obtains shaking flask bacterium liquid;
(4) preparation of bacterium liquid is produced: by 4wt% inoculum size by shaking flask bacterium liquid access fermentor tank, at 30 DEG C, ventilating ratio is 1 ︰ 1 (V/V) liquid amount is ferment under the condition of 1/3, add the substratum continuation fermentation of 1/3 volume again by above-mentioned condition after 48h, stop fermentation after 96h, namely obtain pink yeast liquid.
Phanerochaete chrysosporium bacterium liquid prepares by the following method:
(1) prepare Phanerochaete chrysosporium culture medium raw material, comprise following component and weight percent content: glucose 2.0%, KH
2pO
40.30%, MgSO
47H
2o0.15%, vitaminB10 .01%, agar 1.5%, surplus are potato extracting solution;
(2) preparation of liquid nutrient medium: by Phanerochaete chrysosporium culture medium prescription preparation raw material, at 0.15MPa, 121 DEG C, sterilizing 30min, obtains the liquid nutrient medium of sterilizing, is placed in fermentor tank;
(3) preparation of shaking flask bacterial classification: get the Phanerochaete chrysosporium bacterial classification preserved on inclined-plane and access in sterilized liquid nutrient medium in gnotobasis, at 30 DEG C, rate of shaking 200 times/min, carries out shake-flask culture 48h, obtains shaking flask bacterium liquid;
(4) preparation of bacterium liquid is produced: by 4wt% inoculum size by shaking flask bacterium liquid access fermentor tank, at 30 DEG C, ventilating ratio is 1 ︰ 1 (V/V) liquid amount is ferment under the condition of 1/3, add the substratum continuation fermentation of 1/3 volume again by above-mentioned condition after 48h, stop fermentation after 96h, namely obtain Phanerochaete chrysosporium bacterium liquid.
Bacillus licheniformis liquid prepares by the following method:
(1) Bacillus licheniformis culture medium raw material comprises following component and weight percent content: wheat bran 4.5 ~ 4.7%, soybean cake powder 2.7 ~ 2.85%, agar 1.8 ~ 1.85%, surplus are the NaOH solution of 0.2wt%, after wheat bran, soybean cake powder mixing, add 0.2wt%NaOH solution and boil 1h, neutralizing pH with HCl is 7, then adds agar and melts;
(2) preparation of liquid nutrient medium: sample step (1) obtained is at 0.15MPa, and 121 DEG C, sterilizing 30min, obtains the liquid nutrient medium of sterilizing, be placed in fermentor tank;
(3) preparation of shaking flask bacterial classification: get the Bacillus licheniformis strain preserved on inclined-plane and access in sterilized liquid nutrient medium in gnotobasis, at 30 DEG C, rate of shaking 100 ~ 200 times/min, carries out shake-flask culture 48h, obtains shaking flask bacterium liquid;
(4) preparation of bacterium liquid is produced: by 3wt% ~ 4wt% inoculum size by shaking flask bacterium liquid access fermentor tank, at 30 DEG C, ventilating ratio is 1 ︰ 1 (V/V) liquid amount is ferment under the condition of 1/3, add the substratum continuation fermentation of 1/3 volume again by above-mentioned condition after 48h, stop fermentation after 96h, namely obtain Bacillus licheniformis liquid.
Bacillus pumilus bacterium liquid prepares by the following method:
(1) prepare bacillus pumilus culture medium raw material, comprise following component and weight percent content: peptone 0.6%, beef leaching thing 0.35%, NaCl0.6%, agar 1.5%, surplus are distilled water;
(2) preparation of liquid nutrient medium: by bacillus pumilus culture medium prescription preparation raw material, at 0.15MPa, 121 DEG C, sterilizing 30min, obtains the liquid nutrient medium of sterilizing, is placed in fermentor tank;
(3) preparation of shaking flask bacterial classification: get the bacillus pumilus bacterial classification preserved on inclined-plane and access in sterilized liquid nutrient medium in gnotobasis, at 30 DEG C, rate of shaking 200 times/min, carries out shake-flask culture 48h, obtains shaking flask bacterium liquid;
(4) preparation of bacterium liquid is produced: by 4wt% inoculum size by shaking flask bacterium liquid access fermentor tank, at 30 DEG C, ventilating ratio is 1 ︰ 1 (V/V) liquid amount is ferment under the condition of 1/3, add the substratum continuation fermentation of 1/3 volume again by above-mentioned condition after 48h, stop fermentation after 96h, namely obtain bacillus pumilus bacterium liquid.
Candida maltosa bacterium liquid prepares by the following method:
(1) prepare Candida maltosa culture medium raw material, comprise following component and weight percent content: agar 1.6%; 5 ° of worts 98.4%;
(2) preparation of liquid nutrient medium: by Candida maltosa culture medium prescription preparation raw material, at 0.15MPa, 121 DEG C, sterilizing 30min, obtains the liquid nutrient medium of sterilizing, is placed in fermentor tank;
(3) preparation of shaking flask bacterial classification: get the Candida maltosa bacterial classification preserved on inclined-plane and access in sterilized liquid nutrient medium in gnotobasis, at 30 DEG C, rate of shaking 200 times/min, carries out shake-flask culture 48h, obtains shaking flask bacterium liquid;
(4) preparation of bacterium liquid is produced: by 4wt% inoculum size by shaking flask bacterium liquid access fermentor tank, at 30 DEG C, ventilating ratio is 1 ︰ 1 (V/V) liquid amount is ferment under the condition of 1/3, add the substratum continuation fermentation of 1/3 volume again by above-mentioned condition after 48h, stop fermentation after 96h, namely obtain Candida maltosa bacterium liquid.
Long shoot Trichoderma liquid prepares by the following method:
(1) prepare the mould culture medium raw material of long shoot wood, comprise following component and weight percent content: agar 1.6%; 5 ° of worts 98.4%;
(2) preparation of liquid nutrient medium: by the mould culture medium prescription preparation raw material of long shoot wood, at 0.15MPa, 121 DEG C, sterilizing 30min, obtains the liquid nutrient medium of sterilizing, be placed in fermentor tank;
(3) preparation of shaking flask bacterial classification: get the long shoot Trichoderma kind of preserving on inclined-plane and access in sterilized liquid nutrient medium in gnotobasis, at 30 DEG C, rate of shaking 200 times/min, carries out shake-flask culture 48h, obtains shaking flask bacterium liquid;
(4) preparation of bacterium liquid is produced: by 4wt% inoculum size by shaking flask bacterium liquid access fermentor tank, at 30 DEG C, ventilating ratio is 1 ︰ 1 (V/V) liquid amount is ferment under the condition of 1/3, add the substratum continuation fermentation of 1/3 volume again by above-mentioned condition after 48h, stop fermentation after 96h, namely obtain long shoot Trichoderma liquid.
Complex ferment amino acid imitates nutritive medium in the preparation entirely, after preparing raw material according to formula, raw material pulverizing is puddled evenly, be placed in pottery static fermentation 30 days, then the product obtained is taken out and filter, collect filtrate and control temperature is 110 DEG C carries out high-temperature sterilization, be finally cooled to room temperature, namely obtain complex ferment amino acid and entirely imitate nutritive medium.
Following table by the nutritive ingredient of acquisition biological fermentation facial cleanser.
| Nutrition title | Content (mg/ml) | Nutrition title | Content (mg/ml) |
| Aspartic acid | 0.152 | First sulphur | 0.017 |
| Threonine | 0.035 | V-ABA | 0.097 |
| Serine | 0.023 | Glucose | 0.11％ |
| L-glutamic acid | 0.099 | Maltose | 0.11％ |
| Proline(Pro) | 0.083 | Citric acid | 0.13％ |
| Glycine | 0.084 | VA | 0.13 |
| L-Ala | 0.118 | VB2 | 0.06 |
| Gelucystine | 0.073 | VB6 | 0.09 |
| α-amino-isovaleric acid | 0.111 | VB12 | 0.075 |
| Isoleucine | 0.081 | VC | 0.58 |
| Leucine | 0.126 | VE | 0.12 |
| Tyrosine | 0.015 | Robust fibre | 0.015 |
| Phenylalanine | 0.096 | Zinc | 0.075mg/100g |
| Ornithine | 0.021 | Selenium | 0.89ug/100g |
| Methionin | 0.036 | Iron | 0.045mg/100g |
| Tryptophane | 0.012 | Calcium | 0.085mg/100g |
| Arginine | 0.035 | Soybean isoflavones | 0.23ug/ml |
| Histidine | 0.032 | Soybean saponin | 0.12ug/ml |
| VitB1 | 0.47ug/ml | Soybean phospholipid | 0.24ug/ml |
| Ginsenoside | 0.13ug/ml | β-carotene | 0.32 |
| Ergosterol | 0.08ug/ml |
Claims (2)
1. a biological fermentation facial cleanser, is characterized in that, comprises the component of following weight percentage:
Described complex ferment amino acid is entirely imitated nutritive medium and is obtained by the following method: raw material is placed in pottery static fermentation 21 ~ 30 days, then the product obtained is taken out and filter, collect filtrate and control temperature is 100 ~ 110 DEG C carries out high-temperature sterilization, finally be cooled to room temperature, namely obtain complex ferment amino acid and entirely imitate nutritive medium;
Described raw material comprises the component of following weight percent content: lactic acid bacterial liquid 0.5 ~ 1.0%, pink yeast liquid 0.5 ~ 1.0%, Phanerochaete chrysosporium bacterium liquid 0.5 ~ 1.0%, Bacillus licheniformis liquid 0.5 ~ 1.0%, bacillus pumilus bacterium liquid 0.5 ~ 1.0%, Candida maltosa bacterium liquid 0.5 ~ 1.0%, long shoot Trichoderma liquid 0.5 ~ 1.0%, shatian pomelo 0.4 ~ 0.6%, apple 0.4 ~ 0.6%, banana 0.4 ~ 0.6%, Lac regis apis 0.2 ~ 0.3%, extraordinary soya bean 1.0 ~ 1.2%, ginseng 0.5 ~ 0.9%, glossy ganoderma 0.5 ~ 0.9%, lavandula angustifolia 1.0 ~ 1.2%, rice distiller grain 20 ~ 25%, surplus is pure water,
Described lactic acid bacterial liquid prepares by the following method:
(1) prepare lactic acid bacteria culturing medium raw material, comprise following component and weight percent content: milk casein peptone 8 ~ 12%, peptone 8 ~ 12%, yeast extract paste 4 ~ 6%, glucose 4 ~ 6%, tween-80 0.8 ~ 1.2%, dipotassium hydrogen phosphate 1.8 ~ 2.5%, sodium-acetate 4 ~ 6%, citric acid diamines 1.8 ~ 2.5%, magnesium sulfate 0.1 ~ 0.3%, manganous sulfate 0.04 ~ 0.06%, surplus are pure water;
(2) preparation of liquid nutrient medium: by lactic acid bacteria culturing medium formulated raw material, at 0.15MPa, 121 DEG C, sterilizing 30min, obtains the liquid nutrient medium of sterilizing, is placed in fermentor tank;
(3) preparation of shaking flask bacterial classification: get the lactobacillus inoculation preserved on inclined-plane and access in sterilized liquid nutrient medium in gnotobasis, at 30 DEG C, rate of shaking 100 ~ 200 times/min, carries out shake-flask culture 48h, obtains shaking flask bacterium liquid;
(4) preparation of bacterium liquid is produced: by 3wt% ~ 4wt% inoculum size by shaking flask bacterium liquid access fermentor tank, at 30 DEG C, aeration volume is fermented than under to be 1 ︰ 1 liquid amount the be condition of 1/3, add the sterilized liquid nutrient medium continuation fermentation of 1/3 volume again by above-mentioned condition after 48h, stop fermentation after 96h, namely obtain lactic acid bacterial liquid;
Described pink yeast liquid prepares by the following method:
(1) prepare pink yeast culture based raw material, comprise following component and weight percent content: peptone 4.1 ~ 6%, glucose 8 ~ 12%, yeast extract paste 2 ~ 4%, surplus are wort;
(2) preparation of liquid nutrient medium: by pink yeast culture medium formulated raw material, at 0.15MPa, 121 DEG C, sterilizing 30min, obtains the liquid nutrient medium of sterilizing, is placed in fermentor tank;
(3) preparation of shaking flask bacterial classification: get the pink barms preserved on inclined-plane and access in sterilized liquid nutrient medium in gnotobasis, at 30 DEG C, rate of shaking 100 ~ 200 times/min, carries out shake-flask culture 48h, obtains shaking flask bacterium liquid;
(4) preparation of bacterium liquid is produced: by 3wt% ~ 4wt% inoculum size by shaking flask bacterium liquid access fermentor tank, at 30 DEG C, aeration volume is fermented than under to be 1 ︰ 1 liquid amount the be condition of 1/3, add the substratum continuation fermentation of 1/3 volume again by above-mentioned condition after 48h, stop fermentation after 96h, namely obtain pink yeast liquid;
Described Phanerochaete chrysosporium bacterium liquid prepares by the following method:
(1) prepare Phanerochaete chrysosporium culture medium raw material, comprise following component and weight percent content: glucose 1.9 ~ 2.0%, KH
2pO
40.25 ~ 0.30%, MgSO
47H
2o0.14 ~ 0.15%, vitaminB10 .01%, agar 1.4 ~ 1.5%, surplus are potato extracting solution;
(2) preparation of liquid nutrient medium: by Phanerochaete chrysosporium culture medium prescription preparation raw material, at 0.15MPa, 121 DEG C, sterilizing 30min, obtains the liquid nutrient medium of sterilizing, is placed in fermentor tank;
(3) preparation of shaking flask bacterial classification: get the Phanerochaete chrysosporium bacterial classification preserved on inclined-plane and access in sterilized liquid nutrient medium in gnotobasis, at 30 DEG C, rate of shaking 100 ~ 200 times/min, carries out shake-flask culture 48h, obtains shaking flask bacterium liquid;
(4) preparation of bacterium liquid is produced: by 3wt% ~ 4wt% inoculum size by shaking flask bacterium liquid access fermentor tank, at 30 DEG C, aeration volume is fermented than under to be 1 ︰ 1 liquid amount the be condition of 1/3, add the substratum continuation fermentation of 1/3 volume again by above-mentioned condition after 48h, stop fermentation after 96h, namely obtain Phanerochaete chrysosporium bacterium liquid;
Described Bacillus licheniformis liquid prepares by the following method:
(1) Bacillus licheniformis culture medium raw material comprises following component and weight percent content: wheat bran 4.5 ~ 4.7%, soybean cake powder 2.7 ~ 2.85%, agar 1.8 ~ 1.85%, surplus are the NaOH solution of 0.2wt%, after wheat bran, soybean cake powder mixing, add 0.2wt%NaOH solution and boil 1h, neutralizing pH with HCl is 7, then adds agar and melts;
(2) preparation of liquid nutrient medium: sample step (1) obtained is at 0.15MPa, and 121 DEG C, sterilizing 30min, obtains the liquid nutrient medium of sterilizing, be placed in fermentor tank;
(3) preparation of shaking flask bacterial classification: get the Bacillus licheniformis strain preserved on inclined-plane and access in sterilized liquid nutrient medium in gnotobasis, at 30 DEG C, rate of shaking 100 ~ 200 times/min, carries out shake-flask culture 48h, obtains shaking flask bacterium liquid;
(4) preparation of bacterium liquid is produced: by 3wt% ~ 4wt% inoculum size by shaking flask bacterium liquid access fermentor tank, at 30 DEG C, aeration volume is fermented than under to be 1 ︰ 1 liquid amount the be condition of 1/3, add the substratum continuation fermentation of 1/3 volume again by above-mentioned condition after 48h, stop fermentation after 96h, namely obtain Bacillus licheniformis liquid;
Described bacillus pumilus bacterium liquid prepares by the following method:
(1) prepare bacillus pumilus culture medium raw material, comprise following component and weight percent content: peptone 0.4 ~ 0.6%, beef leaching thing 0.25 ~ 0.35%, NaCl0.4 ~ 0.6%, agar 1.4 ~ 1.5%, surplus are distilled water;
(2) preparation of liquid nutrient medium: by bacillus pumilus culture medium prescription preparation raw material, at 0.15MPa, 121 DEG C, sterilizing 30min, obtains the liquid nutrient medium of sterilizing, is placed in fermentor tank;
(3) preparation of shaking flask bacterial classification: get the bacillus pumilus bacterial classification preserved on inclined-plane and access in sterilized liquid nutrient medium in gnotobasis, at 30 DEG C, rate of shaking 100 ~ 200 times/min, carries out shake-flask culture 48h, obtains shaking flask bacterium liquid;
(4) preparation of bacterium liquid is produced: by 3wt% ~ 4wt% inoculum size by shaking flask bacterium liquid access fermentor tank, at 30 DEG C, aeration volume is fermented than under to be 1 ︰ 1 liquid amount the be condition of 1/3, add the substratum continuation fermentation of 1/3 volume again by above-mentioned condition after 48h, stop fermentation after 96h, namely obtain bacillus pumilus bacterium liquid;
Described Candida maltosa bacterium liquid prepares by the following method:
(1) prepare Candida maltosa culture medium raw material, comprise following component and weight percent content: agar 1.4 ~ 1.6%; 5 ° of worts 98.4 ~ 98.6%;
(2) preparation of liquid nutrient medium: by Candida maltosa culture medium prescription preparation raw material, at 0.15MPa, 121 DEG C, sterilizing 30min, obtains the liquid nutrient medium of sterilizing, is placed in fermentor tank;
(3) preparation of shaking flask bacterial classification: get the Candida maltosa bacterial classification preserved on inclined-plane and access in sterilized liquid nutrient medium in gnotobasis, at 30 DEG C, rate of shaking 100 ~ 200 times/min, carries out shake-flask culture 48h, obtains shaking flask bacterium liquid;
(4) preparation of bacterium liquid is produced: by 3wt% ~ 4wt% inoculum size by shaking flask bacterium liquid access fermentor tank, at 30 DEG C, aeration volume is fermented than under to be 1 ︰ 1 liquid amount the be condition of 1/3, add the substratum continuation fermentation of 1/3 volume again by above-mentioned condition after 48h, stop fermentation after 96h, namely obtain Candida maltosa bacterium liquid;
Described long shoot Trichoderma liquid prepares by the following method:
(1) prepare the mould culture medium raw material of long shoot wood, comprise following component and weight percent content: agar 1.4 ~ 1.6%; 5 ° of worts 98.4 ~ 98.6%;
(2) preparation of liquid nutrient medium: by the mould culture medium prescription preparation raw material of long shoot wood, at 0.15MPa, 121 DEG C, sterilizing 30min, obtains the liquid nutrient medium of sterilizing, be placed in fermentor tank;
(3) preparation of shaking flask bacterial classification: get the long shoot Trichoderma kind of preserving on inclined-plane and access in sterilized liquid nutrient medium in gnotobasis, at 30 DEG C, rate of shaking 100 ~ 200 times/min, carries out shake-flask culture 48h, obtains shaking flask bacterium liquid;
(4) preparation of bacterium liquid is produced: by 3wt% ~ 4wt% inoculum size by shaking flask bacterium liquid access fermentor tank, at 30 DEG C, aeration volume is fermented than under to be 1 ︰ 1 liquid amount the be condition of 1/3, add the substratum continuation fermentation of 1/3 volume again by above-mentioned condition after 48h, stop fermentation after 96h, namely obtain long shoot Trichoderma liquid;
Described apple is the apple selecting the Qinling Mountains or Dalian,
The banana in Yunnan selected by described banana,
Described extraordinary soya bean selects the soya bean into Shaanxi or Heilungkiang.
2. a preparation method for biological fermentation facial cleanser as claimed in claim 1, is characterized in that, the method comprises the following steps:
(1) take by weight percentage: complex ferment amino acid imitates nutritive medium 10% ~ 15% entirely; AES15% ~ 17%; Oligosaccharide malt glycosyl glucoside 2% ~ 3%; Coconut oleoyl amine DEA3% ~ 4%; Glycerine 2.5% ~ 3.5%; AMONYL 380LC 6% ~ 8%; Extract solution from aloe 1.8% ~ 2.2%; Water surplus;
(2) poured in emulsification pot by whole water, be then slowly sprinkled into glycerine, stir after within 15 minutes, being uniformly dispersed, be sprinkled into oligosaccharide malt glycosyl glucoside, stir and be uniformly dispersed for 15 minutes, stirring velocity is 80 revs/min;
(3) dropped into by AES in emulsification pot, dispersed with stirring is even, and heat simultaneously, be warming up to 75 ~ 80 DEG C, be incubated half an hour after raw material all dissolves, stirring velocity is 50 revs/min;
(4) add coconut oleoyl amine DEA to stir and be uniformly dissolved for 15 minutes, stirring velocity is 50 revs/min;
(5) cool to less than 65 DEG C, add AMONYL 380LC, stir, to vacuumize and below slow cooling to 40 DEG C simultaneously, stirring velocity 50 revs/min, each raw material adds rear stirring 10-15 minute, adds a rear raw material again after guarantee stirs;
(6) add complex ferment amino acid successively and entirely imitate nutritive medium, extract solution from aloe, stir, stirring velocity 50 revs/min, each raw material adds rear stirring 10-15 minute, adds a rear raw material again after guarantee stirs;
(7) stirring is cooled to 38 DEG C, stops stirring, and inspection discharging, makes biological fermentation facial cleanser.
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