CN103645087A - Phosphate buffer - Google Patents
Phosphate buffer Download PDFInfo
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- CN103645087A CN103645087A CN201310559261.2A CN201310559261A CN103645087A CN 103645087 A CN103645087 A CN 103645087A CN 201310559261 A CN201310559261 A CN 201310559261A CN 103645087 A CN103645087 A CN 103645087A
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- sulfuric acid
- dilute sulfuric
- phosphate buffer
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- solution
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Abstract
The invention discloses a preparation method of a phosphate buffer. The method particularly comprises the following steps: preparing raw materials of 1-3 parts of sodium chloride, 2-10 parts of distilled water, and 3-10 parts of sodium phosphate tetrahydrate; mixing the above components, adding dilute sulfuric acid, adjusting the pH value to 7.2; performing autoclaved sterilization, then storing at a low temperature; adding a ferroferric oxide solution into the mixed solution, mechanically stirring with a high speed; removing the supernatant; forming an emulsion, cooling again; and storing the product at room temperature. The mass fraction of the dilute sulfuric acid is not more than 30%; high speed stirring is necessary for complete turbidity before the addition of the dilute sulfuric acid. The beneficial effects of the invention are that the operation is simple, the reaction condition is mild, no toxic organic solvent is used, influence factors are few, and very good controlled release effect is realized.
Description
Technical field
The present invention relates to a kind ofly to chemical industry preparation technology, specifically, refer to phosphate buffer.
Background technology
Phosphate buffer (Phosphate Buffered Saline, be called for short PBS) be a buffer solution for biological study of commonly using.PBS can be the english abbreviation of three kinds of solution, respectively phosphate buffered solution (phosphate buffered solution), phosphate buffered saline (PBS) (phosphate buffered saline) and phosphate-buffered sodium (phosphate buffered sodium), its compound method is different, pH value is different, and the biological action of performance is also incomplete same.If no special instructions, biologically conventional PBS is neutral phosphate buffered solution (phosphate buffered solution).It is to contain sodium chloride in a kind of water base salt solusion, phosphate, and (at some formula) potassium chloride and potassium phosphate.Damping fluid contributes to the pH value that keeps constant.The osmotic pressure of solution and ion concentration be close with human body pH (wait and ooze) conventionally.Be mainly used in not affecting in the situation of testing reaction and regulating pH value in some chemical experiments, to allow the chemical reaction of experiment carry out under top condition.Part is for food or feedstuff industry processing, in food or the check of feed mycotoxin
elisa method wash the dilution buffer liquid in plate application or food microorganisms check.
Summary of the invention
For overcoming above-mentioned technical matters, we have proposed following technical scheme:
The collocation method of phosphate buffer, concrete steps are:
Prepare raw material: by sodium chloride: 1-3 part, distilled water 2-10 part, four water and sodium phosphate 3-10 part;
After mentioned component is mixed, add dilute sulfuric acid, its pH value is adjusted to 7.2;
After autoclaving, under low temperature, preserve;
To in solution, add tri-iron tetroxide solution, then mechanical high-speed stirs;
Abandon supernatant;
Form milk, again cold putting;
Under room temperature, preserve.
In the present invention, the massfraction of described dilute sulfuric acid is not higher than 30 percent.
In the present invention, add dilute sulfuric acid to need high-speed stirred before, make it completely muddy.
The invention has the beneficial effects as follows, simple to operate, reaction conditions coincide, and does not use poisonous organic solvent, and influence factor is less, can obtain good controlled-release effect.
Embodiment
The collocation method of phosphate buffer, concrete steps are:
Prepare raw material: by sodium chloride: 0 part of 2 parts, 10 parts of distilled water, four water and sodium phosphate;
After mentioned component is mixed, add dilute sulfuric acid, its pH value is adjusted to 7.2;
After autoclaving, under low temperature, preserve;
To in solution, add tri-iron tetroxide solution, then mechanical high-speed stirs;
Abandon supernatant;
Form milk, again cold putting;
Under room temperature, preserve.
The massfraction of described dilute sulfuric acid 30 percent.
Before adding dilute sulfuric acid, need high-speed stirred, make it completely muddy.
The above; it is only preferably embodiment of the present invention; but protection scope of the present invention is not limited to this; anyly be familiar with those skilled in the art in the technical scope that the present invention discloses; according to technical scheme of the present invention and inventive concept thereof, be equal to replacement or changed, within all should being encompassed in protection scope of the present invention.
Claims (3)
1. the collocation method of phosphate buffer, is characterized in that:
1) prepare raw material: by sodium chloride: 1-3 part, distilled water 2-10 part, four water and sodium phosphate 3-10 part;
2) after mentioned component is mixed, add dilute sulfuric acid, its pH value is adjusted to 7.2;
3), after autoclaving, under low temperature, preserve;
4) will in solution, add tri-iron tetroxide solution, then mechanical high-speed stirs;
5) abandon supernatant;
6) form milk, again cold putting;
7) under room temperature, preserve.
2. the collocation method of phosphate buffer as claimed in claim 1, is characterized in that, the massfraction of described dilute sulfuric acid is not higher than 30 percent.
3. the collocation method of phosphate buffer as claimed in claim 1, is characterized in that, adds dilute sulfuric acid to need high-speed stirred before, makes it completely muddy.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310559261.2A CN103645087A (en) | 2013-11-12 | 2013-11-12 | Phosphate buffer |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310559261.2A CN103645087A (en) | 2013-11-12 | 2013-11-12 | Phosphate buffer |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103645087A true CN103645087A (en) | 2014-03-19 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310559261.2A Pending CN103645087A (en) | 2013-11-12 | 2013-11-12 | Phosphate buffer |
Country Status (1)
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| CN (1) | CN103645087A (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108426761A (en) * | 2017-02-15 | 2018-08-21 | 上海瀚联医疗技术股份有限公司 | A kind of phosphate buffer and preparation method thereof |
| CN108426762A (en) * | 2017-02-15 | 2018-08-21 | 上海瀚联医疗技术股份有限公司 | A kind of configuration method of concentrate phosphate buffer |
| CN109307777A (en) * | 2017-07-28 | 2019-02-05 | 上海瀚联医疗技术股份有限公司 | It is a kind of suitable for glucose/lactic acid analysis instrument blood glucose calibration solution configuration method |
| CN109307769A (en) * | 2017-07-28 | 2019-02-05 | 上海瀚联医疗技术股份有限公司 | A kind of blood glucose solution allocation method for 12mmol/L |
| CN109307770A (en) * | 2017-07-28 | 2019-02-05 | 上海瀚联医疗技术股份有限公司 | A kind of blood glucose calibration solution configuration method |
-
2013
- 2013-11-12 CN CN201310559261.2A patent/CN103645087A/en active Pending
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108426761A (en) * | 2017-02-15 | 2018-08-21 | 上海瀚联医疗技术股份有限公司 | A kind of phosphate buffer and preparation method thereof |
| CN108426762A (en) * | 2017-02-15 | 2018-08-21 | 上海瀚联医疗技术股份有限公司 | A kind of configuration method of concentrate phosphate buffer |
| CN108426761B (en) * | 2017-02-15 | 2021-11-05 | 上海瀚联医疗技术股份有限公司 | Phosphate buffer solution and preparation method thereof |
| CN108426762B (en) * | 2017-02-15 | 2021-11-30 | 上海瀚联医疗技术股份有限公司 | Preparation method of concentrated solution phosphate buffer solution |
| CN109307777A (en) * | 2017-07-28 | 2019-02-05 | 上海瀚联医疗技术股份有限公司 | It is a kind of suitable for glucose/lactic acid analysis instrument blood glucose calibration solution configuration method |
| CN109307769A (en) * | 2017-07-28 | 2019-02-05 | 上海瀚联医疗技术股份有限公司 | A kind of blood glucose solution allocation method for 12mmol/L |
| CN109307770A (en) * | 2017-07-28 | 2019-02-05 | 上海瀚联医疗技术股份有限公司 | A kind of blood glucose calibration solution configuration method |
| CN109307770B (en) * | 2017-07-28 | 2022-06-17 | 上海瀚联医疗技术股份有限公司 | Blood sugar calibration solution preparation method |
| CN109307777B (en) * | 2017-07-28 | 2022-06-17 | 上海瀚联医疗技术股份有限公司 | Blood glucose calibration solution preparation method suitable for glucose/lactic acid analyzer |
| CN109307769B (en) * | 2017-07-28 | 2022-06-28 | 上海瀚联医疗技术股份有限公司 | Preparation method of 12mmol/L blood glucose solution |
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| PB01 | Publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20140319 |
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| WD01 | Invention patent application deemed withdrawn after publication |