CN103626581A - Method for preparing abscisic-acid-containing amino acid water-soluble fertilizer - Google Patents
Method for preparing abscisic-acid-containing amino acid water-soluble fertilizer Download PDFInfo
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Abstract
The invention discloses a method for preparing an abscisic-acid-containing amino acid water-soluble fertilizer and solves the problem that the conventional foliage fertilizer is poor in prevention and treatment effect. The method comprises the following steps of (1) collecting and storing azadirachtin leaves, and adding a decomposition agent for full fermentation; (2) adding water, stirring and leaching the mixture, then performing centrifugation, and collecting filtrate; (3) adding strong acid into the filtrate, and performing hydrolysis under the condition of 40-80 DEG C for 15-22 hours to obtain amino acid mother liquor; (4) adding trace elements into the amino acid mother liquor, and adding a chelating agent into a reaction kettle for full chelating; (5) adding abscisic acid, and uniformly stirring; and (6) performing detection and package to obtain products. The abscisic-acid-containing amino acid water-soluble fertilizer has multiple biological activities of killing insects, bacteria and the like, is harmless to higher animals, is safe to natural enemies of insects and also has the advantage that no medicine can be retained on plants.
Description
Technical field
The present invention is specifically related to a kind of preparation method of the amino acid water-soluble fertilizer containing abscisic acid.
Background technology
All can there is multiple physiological disturbance in various degree in China farm crop, cause the underproduction and quality to decline every year.Wherein tomato late blight, pepper virus disease etc. do not find effectively preventing method so far.Existing foliage fertilizer is not good enough to the prevention effect of these diseases.Therefore be necessary to develop a kind of foliage fertilizer that can prevent above-mentioned multiple diseases and improve the yield and quality.
Summary of the invention
The preparation method who the object of the present invention is to provide a kind of amino acid water-soluble fertilizer containing abscisic acid, solves the not good enough problem of existing foliage fertilizer prevention effect.
The present invention is achieved through the following technical solutions:
A preparation method who contains the amino acid water-soluble fertilizer of abscisic acid, comprises the following steps:
(1) by neem leaf collection, saving, add decomposing agent to carry out abundant fermentative processing;
(2) in the neem leaf after fermentation, add water, stir after leaching in 0.5～2 hour centrifugally, collect filtrate;
(3) to adding strong acid in filtrate and at the Water Under solution 15～22h of 40 ℃～80 ℃, obtaining span amino acid mother liquor;
(4) in span amino acid mother liquor, add trace element, add sequestrant in reactor, to carry out sufficient chelating;
(5) add abscisic acid, stir, mix;
(6) detect, pack, obtain product.
Further, described step (1) fermentation time is 7～10 days; The add-on of described decomposing agent is 3～10% of neem leaf quality.
Again further, the quality and the identical in quality of rear neem leaf of fermenting that in described step (2), add water; The quality that adds strong acid in described step (3) is 8～18% of filtrate quality.
Further,, in described step (4), micro-add-on is 2～20% of amino acid solution quality; Described sequestrant add-on is 5～35% of amino acid solution quality; Sequestrant is citric acid.
In addition, in described step (5), abscisic acid add-on is 0.001～5% of amino acid solution quality.
The present invention has the following advantages and beneficial effect:
Printing chinaberry is Meliaceae Melia arbor, has the multiple biological activitys such as desinsection, sterilization; Have the harmless ，Dui of higher animal natural enemies of insects safelyr, but also do not have advantages of and can remain on plant; And be processed into by printing chinaberry leaf the absorption that foliage fertilizer can promote Plant To Nutrient, again insect is had to certain preventive and therapeutic effect.Through detecting, find also to contain nimbin in neem leaf.Nimbin, for the growth that can suppress germ, has the effect of pre-disease prevention.Neem leaf has easy acquisition, aboundresources, feature with low cost.Its application will be the model of turning waste into wealth.
Abscisic acid, is a plant growth regulators, and the coordinate plant growth factor, has degeneration-resistant inducing action, and plant is when environment stress, and abscisic acid transmits adverse circumstance information at iuntercellular, and inducing plant body produces the resistivity of various replies.Under Soil Drought Stress, abscisic acid starts the signal conduction on blade cell plasma membrane, and inhomogeneous the closing of induction blade face pore reduced moisture transpiration in plant materials and scattered and disappeared, and improves Genes For Plant Tolerance arid ability.Under cold is coerced, abscisic acid active cell resists cold gene, and inducing plant produces Coldproof protein matter.Generally speaking, the plant variety that winter resistance is strong, the kind of its endogenous abscisic acid content a little less than higher than winter resistance; Under disease and pest is coerced, abscisic acid inducing plant blade cell PIN gene activation, produces protease inhibitor (pls) obstruction cause of disease or insect pest and further encroaches on, and avoids being injured or alleviating the extent of injury of plant; Under soil Salt Stress, abscisic acid inducing plant strengthens membrane permeability regulating power, reduces NA+ content in every kilogram of material, improves PEP carboxylase activity, strengthens the salt resistance ability of plant; Under the coercing of poisoning fertilizer damage, the balance of regulating plant endogenous hormones, stops further absorption, and efficient solution is except the detrimentally affect of poisoning fertilizer damage; Under normal growth condition, abscisic acid inducing plant strengthens photosynthesis and absorbs nutritive substance, promotes transhipment and the accumulation of material, improves output, improves quality.
The present invention is processed into span amino acid mother liquor by seal chinaberry leaf, adds on its basis the required trace element of plant and abscisic acid and makes the water-soluble foliage fertilizer containing abscisic acid.Adding of abscisic acid, under plant normal growth condition, can not only strengthen the absorption of photosynthesis and promotion Plant To Nutrient by inducing plant, and can also promote transhipment and the accumulation of nutritive substance; Meanwhile, under plant growth environment mal-condition, can also the potential degeneration-resistant border factor of inducing plant, realize self-protection and repairing effect, resist adverse environment, the generation of prevention physiological disturbance.The present invention, through experiment and demonstration, when sick worm is had to certain prophylactic effect, has promoted the absorption of Plant To Nutrient material, has greatly improved fruit quality and yield, is deeply subject to liking of wide farmers.
Embodiment
Below in conjunction with embodiment, the present invention is further illustrated, but embodiments of the present invention are not limited to this.
Due to farmland life-time service chemical fertilizer, cause soil compaction, water pollution, injure the healthy of people, and the greatly developing of Modern Green agricultural and Organic farming, therefore, various natural sex fertilizer is being developed and used in countries in the world all, reduce the harm of Chemical Fertilizer on Environment, safeguard Agro-ecology balance.
Along with agricultural chemicals and fertilizer constantly make great efforts to serve in the development for agriculture, when making huge contribution, we have also experienced constantly appearing suddenly of agricultural chemicals and fertilizer negative influence, therefore, country is used the restriction of high poison, high residue chemical pesticide and chemical fertilizer, make the preventing and controlling of subterranean pest-insect become the difficult point that crop pests is prevented and treated, be mainly manifested in: pest species is more, difficulty of prevention and cure is larger, colonize in soil, damage to crops underground part (comprising the seed of sowing) or crop part near the ground, caused serious loss to peasant.Therefore, market active demand a kind of in control subterranean pest-insect " medicine fertile " of safety and environmental protection again.For guaranteeing that China's agricultural can better develop, remove the defect of agricultural chemicals and fertilizer, in conjunction with the promoter action of utilizing agricultural chemicals and fertilizer to agricultural development, we start to attempt the advantage of agricultural chemicals and fertilizer to combine, research and development " medicine is fertile ".
Medicine fertilizer is to have to kill to press down diseases and pests of agronomic crop or plant growth one or more the function in regulating, and can nutrition is provided or the nutrition of providing is provided simultaneously and improve the multi-Functional Fertilizers of fertilizer and agricultural chemicals utilization ratio for farm crop.Both at home and abroad chemical fertilizer and agricultural chemicals expert think the Application and Development of medicine fertilizer, generally have the superiority of following 6 aspects: balance fertilizing, nutrition are complete; Broad-spectrum high efficacy, effect is lasting; Front control is short afterwards, strengthens resistance; Fertile medicine in conjunction with, do synergy mutually; Easy and simple to handle, use safety; Saving of labor's abridged edition, increasing both production and income.
Fertilizer and agricultural chemicals are combined, and are the new regions of agricultural chemicals and fertilizer future development, will become an important research direction and development trend.And neem biotic insecticide and fertilizer are combined, research and develop into seal chinaberry " medicine is fertile ", this will become domestic initiation, and its technology is in domestically leading.This innovation is not only conducive to protection of the environment, promotes industry cycle development, and can also promote the deep exploitation of Neem industry, expands the advantages for development of nimbin, can not affect again the performance of fertilizer efficiency by desinsection, meanwhile, also compares safety and environmental protection.So applicant proposes to using amino acid that neem leaf leaching makes as mother liquor, adds trace element and abscisic acid, the water-soluble fertilizer fully stir, chelating forming insect protected.Specific as follows:
Embodiment 1
A preparation method who contains the amino acid water-soluble fertilizer of abscisic acid, comprises the following steps:
(1) 1 ton of neem leaf is evenly stacked, added decomposing agent 30kg to carry out fermentative processing, fermentation time is 7～10 days, does not have foul smell to be abundant fermentation;
(2) in the neem leaf after fermentation, add the water identical in quality with neem leaf, stir after leaching in 1～2 hour centrifugally, collect filtrate;
(3) to adding sulfuric acid in filtrate and at the Water Under solution 15～22h of 50 ℃, obtaining span amino acid mother liquor;
(4) in span amino acid mother liquor, add the raw material (can add a kind or several, according to Different Crop, micro-demand be adjusted) that contains copper ferrimanganic zinc boron molybdenum trace element, then add citric acid in reactor, to carry out sufficient chelating;
(5) add abscisic acid, stir, mix;
(6) detect, pack, obtain product.Containing micro-raw material add-on, be wherein 4% of amino acid solution quality, the add-on of citric acid is 10% of amino acid solution quality, and the add-on of abscisic acid is 0.9% of amino acid solution quality, and the add-on of sulfuric acid is 15% of filtrate quality.
Embodiment 2
A preparation method who contains the amino acid water-soluble fertilizer of abscisic acid, comprises the following steps:
(1) 1 ton of neem leaf is evenly stacked, added decomposing agent 50kg to carry out fermentative processing, fermentation time is 7～10 days, does not have foul smell to be abundant fermentation;
(2) in the neem leaf after fermentation, add the water identical in quality with neem leaf, stir after leaching in 2 hours centrifugally, collect filtrate;
(3) to adding hydrochloric acid in filtrate and at the Water Under solution 15～22h of 80 ℃, obtaining span amino acid mother liquor;
(4) in span amino acid mother liquor, add the raw material that contains copper iron microelement, then add citric acid in reactor, to carry out sufficient chelating;
(5) add abscisic acid, stir, mix;
(6) detect, pack, obtain product.Containing micro-raw material add-on, be wherein 2% of amino acid solution quality, the add-on of citric acid is 5% of amino acid solution quality, and the add-on of abscisic acid is 0.01% of amino acid solution quality, and the add-on of hydrochloric acid is 13% of filtrate quality.
Embodiment 3
A preparation method who contains the amino acid water-soluble fertilizer of abscisic acid, comprises the following steps:
(1) 1 ton of neem leaf is evenly stacked, added decomposing agent 100kg to carry out fermentative processing, fermentation time is 7～9 days, does not have foul smell to be abundant fermentation;
(2) in the neem leaf after fermentation, add the water identical in quality with neem leaf, stir after leaching in 1 hour centrifugally, collect filtrate;
(3) to adding nitric acid in filtrate and at the Water Under solution 15～22h of 40 ℃, obtaining span amino acid mother liquor;
(4) in span amino acid mother liquor, add the raw material that contains MnZn trace element, then add citric acid in reactor, to carry out sufficient chelating;
(5) add abscisic acid, stir, mix;
(6) detect, pack, obtain product.Containing micro-raw material add-on, be wherein 20% of amino acid solution quality, the add-on of citric acid is 35% of amino acid solution quality, and the add-on of abscisic acid is 5% of amino acid solution quality, and the add-on of nitric acid is 13% of filtrate quality.
Embodiment 4
A preparation method who contains the amino acid water-soluble fertilizer of abscisic acid, comprises the following steps:
(1) 1 ton of neem leaf is evenly stacked, added decomposing agent 80kg to carry out fermentative processing, fermentation time is 7～9 days, does not have foul smell to be abundant fermentation;
(2) in the neem leaf after fermentation, add the water identical in quality with neem leaf, stir after leaching in 1.5 hours centrifugally, collect filtrate;
(3) to adding strong acid in filtrate and at the Water Under solution 15～22h of 70 ℃, obtaining span amino acid mother liquor;
(4) in span amino acid mother liquor, add the raw material that contains boron molybdenum trace element, then add citric acid in reactor, to carry out sufficient chelating;
(5) add abscisic acid, stir, mix;
(6) detect, pack, obtain product.Containing micro-raw material add-on, be wherein 10% of amino acid solution quality, the add-on of citric acid is 18% of amino acid solution quality, and the add-on of abscisic acid is 2.5% of amino acid solution quality, and the add-on of strong acid is 8% of filtrate quality.
Embodiment 5
A preparation method who contains the amino acid water-soluble fertilizer of abscisic acid, comprises the following steps:
(1) 1 ton of neem leaf is evenly stacked, added decomposing agent 60kg to carry out fermentative processing, fermentation time is 7～9 days, does not have foul smell to be abundant fermentation;
(2) in the neem leaf after fermentation, add the water identical in quality with neem leaf, stir after leaching in 2 hours centrifugally, collect filtrate;
(3) to adding strong acid in filtrate and at the Water Under solution 15～22h of 70 ℃, obtaining span amino acid mother liquor;
(4) in amino acid solution, add the raw material that contains boron molybdenum trace element, then add citric acid in reactor, to carry out sufficient chelating;
(5) add abscisic acid, stir, mix;
(6) detect, pack, obtain product.Containing micro-raw material add-on, be wherein 15% of amino acid solution quality, the add-on of citric acid is 20% of amino acid solution quality, and the add-on of abscisic acid is 4% of amino acid solution quality, and the add-on of strong acid is 10% of filtrate quality.
The amino acid water-soluble fertilizer that the present invention prepares, its index meets industry standard, and free aminoacid content also meets corresponding index.The present invention in use, need to dilute spraying according to the weaker concn of 500～1000 times by product, pre-preventing disease and pest when providing nutrition to plant.
Field test of the present invention
1, for studying thing: paddy rice (kind is D excellent 527).
2, test method:
Local conventional fertilizer application: 15.0 kilograms, urea of mu, monoammonium phosphate 11.4 kilograms of (N>=11.0%, P before rice transplanting
2o
5>=44.0%), 15 kilograms of (P of Repone K
2o
5>=60.0%) make base fertilizer, while turning green, every mu is topdressed with 5 kilograms, urea.
1. represent conventional fertilizer application+clear water
Tillering phase, boot stage, filling stage are used 60 kilograms of even spraying of clear water at every turn, and all the other are with conventional.
2. show the product that conventional fertilizer application+the present embodiment 1 is prepared
Tillering phase, boot stage, each every mu of use of filling stage are watered 60 kilograms of even spraying containing abscisic acid propylhomoserin amino acid water-soluble fertilizer 100ml, and all the other are with conventional.
3, results and analysis
Table 1 biological economy character analysis
Unit: ten thousand mu of centimetres of ten thousand fringe grains gram kilogram
Known according to table 1,5 observations with field simultaneous test are recorded result and are shown, spray the paddy rice of the product of the present embodiment 1 preparation, and plant growing way is vigorous, tillers fast, and the percentage of earbearing tiller is high.Compare with control group, Gao Miao, plant height, spike length, number of productive ear, grain number per spike, thousand seed weight increase by 1.84 ten thousand seedlings, 0.6 centimetre, 0.17 centimetre, 0.2 ten thousand fringes, 3.9,0.1 gram than control group respectively.
The yield comparison result of table 2 capsicum
Unit: hectare/mu
Known according to table 2, paddy rice sprays water-soluble fertilizer of the present invention, and mean yield reaches 546.1 kilograms, compares 33.18 kilograms of average yield per mu volume increase with control group, and stimulation ratio reaches 6.5%.
Should be noted that, above embodiment is the unrestricted technical scheme of the present invention in order to explanation only, although the present invention is had been described in detail with reference to above-described embodiment, those of ordinary skill in the art is to be understood that: still can modify or be equal to replacement the present invention, and not departing from any modification or partial replacement of the spirit and scope of the present invention, it all should be encompassed in claim scope of the present invention.
Claims (6)
1. contain a preparation method for the amino acid water-soluble fertilizer of abscisic acid, it is characterized in that, comprise the following steps:
(1) by neem leaf collection, saving, add decomposing agent to carry out abundant fermentative processing;
(2) in the neem leaf after fermentation, add water, stir after leaching in 0.5～2 hour centrifugally, collect filtrate;
(3) to adding strong acid in filtrate and at the Water Under solution 15～22h of 40 ℃～80 ℃, obtaining span amino acid mother liquor;
(4) in span amino acid mother liquor, add trace element, add sequestrant in reactor, to carry out sufficient chelating;
(5) add abscisic acid, stir, mix;
(6) detect, pack, obtain product.
2. the preparation method of a kind of amino acid water-soluble fertilizer containing abscisic acid according to claim 1, is characterized in that, described step (1) fermentation time is 7～10 days; The add-on of described decomposing agent is 3～10% of neem leaf quality.
3. the preparation method of a kind of amino acid water-soluble fertilizer containing abscisic acid according to claim 1, is characterized in that, adds the quality and the identical in quality of rear neem leaf of fermenting of water in described step (2).
4. the preparation method of a kind of amino acid water-soluble fertilizer containing poly aspartic acid according to claim 1, is characterized in that, the quality that adds strong acid in described step (3) is 8～18% of filtrate quality.
5. the preparation method of a kind of amino acid water-soluble fertilizer containing abscisic acid according to claim 1, is characterized in that, in described step (4), micro-add-on is 2～20% of amino acid solution quality; Described sequestrant add-on is 5～35% of amino acid solution quality; Sequestrant is citric acid.
6. the preparation method of a kind of amino acid water-soluble fertilizer containing abscisic acid according to claim 1, is characterized in that, in described step (5), abscisic acid add-on is 0.001～5% of amino acid solution quality.
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Cited By (3)
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| CN104876717A (en) * | 2015-05-19 | 2015-09-02 | 潍坊友容实业有限公司 | Water flush fertilizer containing azadirachtin for saline-alkali soil and application of water flush fertilizer in sea-buckthorn planting |
| CN104892126A (en) * | 2015-05-19 | 2015-09-09 | 潍坊友容实业有限公司 | A plant-regulator-containing fertilizer for saline-alkali land and applications thereof in plantation of euonymus fortunei |
| CN110683913A (en) * | 2019-10-21 | 2020-01-14 | 武汉楚强生物科技有限公司 | Amino acid-containing fertilizer based on virus-containing insect residue hydrolysate and preparation method thereof |
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Cited By (3)
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| CN104876717A (en) * | 2015-05-19 | 2015-09-02 | 潍坊友容实业有限公司 | Water flush fertilizer containing azadirachtin for saline-alkali soil and application of water flush fertilizer in sea-buckthorn planting |
| CN104892126A (en) * | 2015-05-19 | 2015-09-09 | 潍坊友容实业有限公司 | A plant-regulator-containing fertilizer for saline-alkali land and applications thereof in plantation of euonymus fortunei |
| CN110683913A (en) * | 2019-10-21 | 2020-01-14 | 武汉楚强生物科技有限公司 | Amino acid-containing fertilizer based on virus-containing insect residue hydrolysate and preparation method thereof |
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Application publication date: 20140312 Assignee: SICHUAN GUOKE ZHONGNONG BIOTECHNOLOGY CO., LTD. Assignor: Chengdu Lujin Biological Sci-Tech Co., Ltd. Contract record no.: 2018510000065 Denomination of invention: Method for preparing abscisic-acid-containing amino acid water-soluble fertilizer Granted publication date: 20160427 License type: Common License Record date: 20181115 |
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Denomination of invention: A preparation method of amino acid water-soluble fertilizer containing inducer Effective date of registration: 20220128 Granted publication date: 20160427 Pledgee: Bank of China Limited Chengdu Longquanyi sub branch Pledgor: CHENGDU LYUJIN BIOLOGICAL SCIENCE & TECHNOLOGY CO.,LTD. Registration number: Y2022510000033 |