CN103621481B - Indoor evaluating method for repellant effects of pest repellents - Google Patents
Indoor evaluating method for repellant effects of pest repellents Download PDFInfo
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- CN103621481B CN103621481B CN201310655046.2A CN201310655046A CN103621481B CN 103621481 B CN103621481 B CN 103621481B CN 201310655046 A CN201310655046 A CN 201310655046A CN 103621481 B CN103621481 B CN 103621481B
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Abstract
The invention discloses an indoor evaluating method for repellant effects of pest repellents. According to the method, host plant branches which are treated by the pest repellants and are used as the control are respectively placed in branch cups arranged at the two sides of an experiment pest cage, then, sufficient experiment pests are fed from a pest feeding opening, the experiment pest cage is placed into an artificial climate box suitable for experiment pests to live, and the repellant effects of the pest repellents are observed after the preset time. A middle separation plate is arranged in the middle of the experiment pest cage for diving the experiment pest cage to form a left side pest cage and a right side pest cage, a pest feeding groove is formed in the top of the middle separation plate and is respectively communicated with the left side pest cage and the right side pest cage through a test pest outlet, the pest feeding opening capable of being opened and closed is formed in the cage wall above the pest feeding groove, screen ventilation openings are respectively formed in the side walls of the left and right side pest cages, screen covers are respectively arranged on the tops of the left and right side pest cages, and the branch cups capable of being used for placing host plant branches are also arranged in the left and right pest cages.
Description
Technical field:
The present invention relates to a kind of indoor evaluation method of insect repellent, be specifically related to the indoor evaluation method of the insects repellant repellent effects such as a kind of diaphorina citri (Diaphorina citriKuwayama).
Background technology:
Common insect pests repellant can be used for skin, makes skin exempt from mosquito, mite, lice, the biting of worm; Also can be used for packaging, prevent storage product from suffering the destruction of insect.Current, insects repellant application in control sanitary insect pest is comparatively general, as culicifuge and cockroach repellant etc.Further, the repellant for indoor sanitary insect pest has relevant evaluation method (as GB/T13917.9-2009 agriculture chemical registration hygienic insecticide indoor pharmacodynamic test and evaluation the 9th part: repellant).
Compare with sanitary insect pest repellant with insecticide, the repellant development of control agriculture and forestry injurious insect is comparatively slow.But along with the raising of people's environmental consciousness and the reinforcement to own health concern consciousness, the cry of green agriculture is more and more higher, and insects repellant will play the part of more key player in control pest.In the process that the mankind and pest struggle, the idea of agricultural insect pests control is also progressively being changed.Laying eggs or taking food by pest, reaches the object of Control pests, and this method more and more comes into one's own.
Therefore the repellant preventing and treating pest will have wide market prospects.Repellant is as a kind of special application field of insecticide, and its application potential has to be excavated.The research of this respect just more and more comes into one's own.In recent decades, the repellent activity material of Chinese scholars to agriculture and forestry injurious insect has done a large amount of research work, also the many activated compositions of extracting from a large amount of plant.Two class materials can be divided into by preventing and treating function, have take food repellant and the large class of oviposition detterent agent two can being divided into of repellent action to insect.
It should be noted that does not have good System Assessment Method for agriculture and forestry injurious insect.
Summary of the invention:
The object of this invention is to provide a kind of indoor evaluation method of insects repellant repellent effect.
The indoor evaluation method of insects repellant repellent effect of the present invention, it is characterized in that, place respectively in the branch cup of the both sides of experiment worm cage through insects repellant process and host plant branch in contrast, then enough experiment insects are accessed connecing insect population, then experiment worm cage is positioned over experiment insect to be applicable to, in the climatic cabinate of existence, observing the repellent effect of insects repellant through predetermined time;
Described experiment worm cage, be provided with central dividing plate therebetween and will test worm cage separately formation left and right side worm cage, be provided with at the top of central dividing plate and connect worm groove, connect worm groove by communicating with left and right side worm cage respectively for the outlet of examination worm, connect the cage wall above worm groove is provided with can folding connect insect population, the sidewall of left and right side worm cage is all provided with grenadine ventilating opening, the top of left and right side worm cage is all provided with band grenadine lid, is also provided with the branch cup can placing host plant branch in left and right side worm cage.
The described angle between the outlet of examination worm that supplies leading to left and right side worm cage is preferably acute angle.
Described is experiment worm taxis behavior outlet for the outlet of examination worm, and wide 1 ~ 2mm, is highly less than 5mm, as 4mm etc.
Described experiment worm cage is preferably the experiment worm cage that transparent material makes, and as made with transparent plastic material or glass, is easy to the observation of testing insect.
Compared with prior art, beneficial effect of the present invention is as follows:
1) compared with existing Y-piece, present invention reduces the distance of creeping of experiment insect, contribute to the observation of experimental result like this, the special insect carrying out movement by jump.
2) be spaced apart both sides from centre, effectively stop process branch to the interference of contrast branch, improve the accuracy of repellant laboratory experiment.
Accompanying drawing illustrates:
Fig. 1 is the structural representation of experiment worm cage of the present invention;
Fig. 2 is the vertical view of the experiment worm cage in Fig. 1;
1, insect population is connect; 2, for the outlet of examination worm; 3, worm groove is connect; 4, central dividing plate; 5, grenadine ventilating opening; 6, host plant branch; 7, right side worm cage; 8, branch cup; 9, grenadine lid is with; 10, left side worm cage.
Embodiment:
Following examples further illustrate of the present invention, instead of limitation of the present invention.
Embodiment 1:
As shown in Figure 1, the experiment worm cage of the present embodiment, it is by transparent plastic production, be provided with central dividing plate 4 therebetween and experiment worm cage is separately formed a left side, right both sides worm cage, i.e. left side worm cage 10 and right side worm cage 7, be provided with at the top of central dividing plate and connect worm groove 3, connect worm groove 3 by communicating with left side worm cage 10 and right side worm cage 7 respectively for examination worm outlet 2, the described angle tried between worm outlet that supplies leading to left side worm cage 10 and right side worm cage 7 is acute angle, connect the cage wall above worm groove 3 is provided with can folding connect insect population 1, the sidewall of left side worm cage 10 and right side worm cage 7 is all provided with grenadine ventilating opening 5 respectively, the sidewall of left side worm cage 10 and right side worm cage 7 is also all provided with band grenadine lid 9 respectively, the sidewall of left side worm cage 10 and right side worm cage 7 is all provided with the branch cup 8 can placing host plant branch 6 respectively.
Place in the branch cup 8 of the both sides of experiment worm cage the host plant kamuning branch 6 processing and contrast clear water process through diaphorina citri repellant (active ingredient is guava extract and paraffine base crude oil) respectively, then access 60 diaphorina citri (female-male proportion is 1:1) connecing insect population.2 will to climb out of to both sides kamuning branch from for the outlet of examination worm connecing worm groove 3 for examination diaphorina citri.Experiment worm cage is positioned in the climatic cabinate of applicable diaphorina citri existence.Through the time of overtesting setting, observe the stop situation of diaphorina citri on the kamuning branch of both sides.
Concrete outcome is as follows:
Table 1: the Lab-evaluation of diaphorina citri repellent effect
| Side worm cage | Opposite side worm cage |
| Kamuning branch is through repellant ①Process | Clear water control treatment |
| 80±9％ | 14±9％ |
| Clear water control treatment | Kamuning branch is through repellant ②Process |
| 9±6％ | 88±6％ |
| Blank (kamuning branch is without chemicals treatment) | Blank (kamuning branch is without chemicals treatment) |
| 46±9％ | 47±10％ |
1. 1L repellant is containing 20g guava extract+10ml 95% (volume fraction) paraffine base crude oil (aqueous solution);
2. 1L repellant is containing 30g guava extract+20ml 95% (volume fraction) paraffine base crude oil (aqueous solution).
As can be seen from Table 1, of the present invention can well Lab-evaluation diaphorina citri repellant walk quickly and keep away effect.
Claims (4)
1. the indoor evaluation method of an insects repellant repellent effect, it is characterized in that, place respectively in the branch cup of the both sides of experiment worm cage through insects repellant process and host plant branch in contrast, then enough experiment insects are accessed connecing insect population, experiment worm cage is positioned over experiment insect be applicable to, in the climatic cabinate of existence, observing the repellent effect of insects repellant through predetermined time;
Described experiment worm cage, be provided with central dividing plate therebetween and will test worm cage separately formation left and right side worm cage, be provided with at the top of central dividing plate and connect worm groove, connect worm groove by communicating with left and right side worm cage respectively for the outlet of examination worm, connect the cage wall above worm groove is provided with can folding connect insect population, the sidewall of left and right side worm cage is all provided with grenadine ventilating opening, the top of left and right side worm cage is all provided with band grenadine lid, is also provided with the branch cup can placing host plant branch in left and right side worm cage.
2. indoor evaluation method according to claim 1, is characterized in that, the described angle tried between worm outlet that supplies leading to left and right side worm cage is acute angle.
3. indoor evaluation method according to claim 1, is characterized in that, described is experiment worm taxis behavior outlet for the outlet of examination worm, and wide 1 ~ 2mm, is highly less than 5mm.
4. indoor evaluation method according to claim 1, is characterized in that, described experiment worm cage is the experiment worm cage that transparent material makes.
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| CN103621481B true CN103621481B (en) | 2015-07-01 |
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Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108072735A (en) * | 2016-11-10 | 2018-05-25 | 成都彩虹电器（集团）股份有限公司 | A kind of spatial repellency product indoor pharmacodynamic test method |
| CN107515294A (en) * | 2017-08-14 | 2017-12-26 | 无限极（中国）有限公司 | The method of testing of repellent worm effect |
| TWI710318B (en) * | 2020-01-15 | 2020-11-21 | 國立高雄大學 | Drug effect test device for mosquito repellent |
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| CN1100331A (en) * | 1994-04-26 | 1995-03-22 | 上海市徐汇区郑氏电化学服务部 | Multi-function, long-acting antiscolic disinfectant and its preparing method |
| CN101084745A (en) * | 2007-06-15 | 2007-12-12 | 黄竹 | Flower dock mosquito-killing device |
| CN101347111A (en) * | 2008-08-27 | 2009-01-21 | 浙江省农业科学院 | Trap for perfect insect of diamondback moth |
| CN102132693A (en) * | 2010-10-15 | 2011-07-27 | 黄竹 | Mosquito dispeller for sunken flowerbed |
| CN102326593A (en) * | 2011-09-19 | 2012-01-25 | 华南农业大学 | Application of plant essential oil serving as red imported fire ant repellent |
| CN102640765A (en) * | 2012-04-18 | 2012-08-22 | 王振伟 | Green and novel crop pest repellent |
| CN202456203U (en) * | 2011-07-11 | 2012-10-03 | 中国人民解放军疾病预防控制所 | Repellant efficacy testing device |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR101097893B1 (en) * | 2009-03-06 | 2011-12-23 | (주)카스트엔지니어링 | Using LED illumination exterminate machine pests |
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Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1046441A (en) * | 1990-06-16 | 1990-10-31 | 北京市东风制药厂研究所 | The mosquito-repellent that contains crude vegetal |
| CN1100331A (en) * | 1994-04-26 | 1995-03-22 | 上海市徐汇区郑氏电化学服务部 | Multi-function, long-acting antiscolic disinfectant and its preparing method |
| CN101084745A (en) * | 2007-06-15 | 2007-12-12 | 黄竹 | Flower dock mosquito-killing device |
| CN101347111A (en) * | 2008-08-27 | 2009-01-21 | 浙江省农业科学院 | Trap for perfect insect of diamondback moth |
| CN102132693A (en) * | 2010-10-15 | 2011-07-27 | 黄竹 | Mosquito dispeller for sunken flowerbed |
| CN202456203U (en) * | 2011-07-11 | 2012-10-03 | 中国人民解放军疾病预防控制所 | Repellant efficacy testing device |
| CN102326593A (en) * | 2011-09-19 | 2012-01-25 | 华南农业大学 | Application of plant essential oil serving as red imported fire ant repellent |
| CN102640765A (en) * | 2012-04-18 | 2012-08-22 | 王振伟 | Green and novel crop pest repellent |
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Address after: 510260 Xingang West Road, Guangdong, Guangzhou, No. 105, No. Patentee after: Guangdong Institute of biological resources application Address before: 510260 Xingang West Road, Guangdong, Guangzhou, No. 105, No. Patentee before: Guangdong Entomological Institute |
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Address after: 510260 No. 105 West Xingang Road, Guangzhou, Guangdong, Haizhuqu District Patentee after: Institute of zoology, Guangdong Academy of Sciences Address before: 510260 No. 105 West Xingang Road, Guangzhou, Guangdong, Haizhuqu District Patentee before: Guangdong Institute of Applied Biological Resources |