CN103609897B - Preparation method and application of Chinese herbal immunopotentiator for Salmo trutta marmoratus - Google Patents
Preparation method and application of Chinese herbal immunopotentiator for Salmo trutta marmoratus Download PDFInfo
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- CN103609897B CN103609897B CN201310602923.XA CN201310602923A CN103609897B CN 103609897 B CN103609897 B CN 103609897B CN 201310602923 A CN201310602923 A CN 201310602923A CN 103609897 B CN103609897 B CN 103609897B
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Abstract
The invention discloses a fermented Chinese herbal immunopotentiator for Salmo trutta marmoratus. The fermented Chinese herbal immunopotentiator has various effects such as prevention, healthcare, treatment, immunoregulation and the like so as to be a novel ideal green safe feed additive capable of substituting antibiotic drugs. The fermented Chinese herbal feed additive is prepared from a compound Chinese herbal medicine through a process comprising the steps: carrying out ultrasonic-assisted extraction on a Chinese herbal medicine, and carrying out two-step fermentation on multiple strains, so that the fermented Chinese herbal feed additive has double advantages of the Chinese herbal medicine and probiotics and can be used for efficiently regulating the intestinal health of the Salmo trutta marmoratus, treating both symptoms and root causes, consolidating the basis, keeping the intestinal microecology balanced and in the optimal immune state, increasing the digestibility of nutrient substances, improving the immunity of the organism of the Salmo trutta marmoratus, increasing the breeding income and reducing the breeding risk; and the fermented Chinese herbal immunopotentiator is used for replacing antibiotics and is free of adverse effects to the Salmo trutta marmoratus, safe in use and free of the problems such as drug residues, drug resistance and the like so as to have a wide market prospect.
Description
Technical field
The preparation method who the present invention relates to a kind of spot trout salmon Chinese medicine immunity enhancer, belongs to feed additive field.
Background technology
Aquatic products more and more occupy important share in people's diet, in order to meet the demand of ever-increasing aquatic products, solving because aquaculture scale constantly expands and growth cycle reduces the physiological function disorder, the disease that cause and takes place frequently and become the Important Problems of aquaculture, is also the key of aquatic feeds industry development.Under this background, antibiotics feed addictive is subject to heat and holds in both hands, and is widely used in feed industry, has promoted to a certain extent the development of culture fishery, but more serious of the problems such as the high residue that antibiotic brings, high pollution and the resistance to the action of a drug.Current social, food security Frequent Accidents, people also more focus on healthy diet in satisfied having enough to eat and wear, and the aquatic products of green health receive much concern.Chinese herbal feed additive meets the requirement of green aquatic products aquaculture development, has eliminated the safety worries of people to its aquatic products product of feeding out, should vigorously promote the use.But the research level of current Chinese herbal feed additive is lower, manufacture craft simple coarse, the technological element of a product is lower, and quality is stable not, and problem like that is seriously restricting applying of Chinese medicine fodder interpolation.Microorganism fermented tcm is reform and the innovation to traditional Chinese medicine process technology, has various features and advantage.This technology is introduced to the research of Chinese herbal feed additive, can make its scientific and technological content significantly promote, effect is more remarkable, quality is more stable, benefit is more obvious, can significantly improve immunity of organisms, strengthen the level of aquatic livestock resist the disease, have important using value for the various problems that solves aquatic livestock in current culture fishery.Utilize microbial fermentation technology to process Chinese herbal medicine, can make the content of its main active substances polysaccharide significantly improve, this seems particularly important for the culture fishery of focusing on aquaculture cost.
Spot trout salmon, be commonly called as " honorable fish ", because being covered with spot, whole body gains the name, to cultivate by Atlantic salmon and the rainbow trout hybridization of artificially breeding the triploid trout salmon class forming, new varieties that belong to salmon, adult maximum can reach 75 centimetres, and body weight reaches more than 18 kilograms, mainly be distributed in all states in North America, because high yield also has a large amount of cultivation in countries such as Norway, Sweden, Chile.Spot trout salmon fine and tender taste, mouthfeel uniqueness, the thorn between (high protein, higher fatty acid, low cholesterol, content of highly unsaturated fatty acids are high), few ridge that is of high nutritive value, dressing percentage are high, the first-selected fish of making raw fish, sootiness salmon, market outlet is good, is one of fish most popular on Europe, Han Deng state of America and Japan dining table.In addition, spot trout salmon also has unique medical value, contain the glycine that general fish do not have, the content of vitamin A, Cobastab and ferro element is also apparently higher than other fish, other fish are incomparable especially for the content of highly unsaturated fatty acid (EPA, EHA, DHA), can effectively prevent the generation of cardiovascular and cerebrovascular disease, the effectively cancer of opposing diabetes chronic diseases and some type, promote infant's brain development, the healing of accelerated in wounds, has wide DEVELOPMENT PROSPECT.But spot trout salmon is after China propagates artificially successfully at present, also exist larger cultivation risk, particularly cultivate survival rate lower, strengthen spot trout salmon and become reduction cultivation risk, improve cultivation income, promote the key of spot trout salmon aquaculture sustainable development.
Summary of the invention
The object of the invention is to according to above shortcomings in prior art, consider antibacterial, antiviral, the left and right of improving immunity of organisms and improving growth of animal performance that Chinese herbal medicine has, can reduce a large amount of aquacultures that cause that use of antibiotic pollutes, and raw material sources are wide, cheap, be a kind of green, environmental protection, efficient feed addictive, develop a kind of spot trout salmon Chinese medicine immunity enhancer.
Above-mentioned purpose of the present invention is achieved by the following technical programs:
A kind of spot trout salmon Chinese medicine immunity enhancer, is characterized in that compound Chinese herb is made up of the Chinese herbal medicine of following mass ratio: 12～18 parts of gynostemma pentaphylla, 8～12 parts of Huang Ling, 8～12 parts of hawthorn, 6～10 parts of Radix Isatidis, 3～5 parts of polygala roots, 6～10 parts of cordate houttuynias, 6～8 parts of the barks of eucommia, 3～5 parts, Radix Glycyrrhizae, old 6～10 parts of bacterium, 6～10 parts of the seed of Chinese dodders, 6～10 parts, cactus, 5～7 parts of glossy ganodermas, 3～5 parts of barrenwort.
The invention provides a kind of spot trout salmon Chinese medicine immunity enhancer, selected leavening is by bacillus subtilis (Chinese agriculture microorganism fungus kind preservation administrative center, bacterium numbering ACCC10619), S. cervisiae (Angel Yeast), lactobacillus bulgaricus (Chinese agriculture microorganism fungus kind preservation administrative center, bacterium numbering ACCC10638) and bifidobacterium bifidum (ATCC standard bacterial classification, bacterium numbering ATCC29521) composition composite ferment, wherein bacillus subtilis, lactobacillus bulgaricus, the inoculum concentration of bifidobacterium bifidum is 2～4%, S. cervisiae inoculum concentration is 5～8%.
The preparation method who the invention provides a kind of spot trout salmon Chinese medicine immunity enhancer, specifically comprises the following steps:
(1) preparation of compound Chinese herb: accurately weigh Chinese herbal medicine according to above-mentioned formula, be crushed to 40～60 orders, mix, make compound Chinese herb;
(2) Ultrasonic Wave-Assisted Extraction: to the water that adds 5～10 times in compound Chinese herb pulvis, under ultrasonic wave booster action, lixiviate 20～30min refluxes under 100 DEG C of conditions;
(3) zymotic fluid preparation: add 3.0～5.0% soluble starches, 2.0～3.0% beancake powders, 0.3～0.5% potassium dihydrogen phosphate, 0.02%MgSO in the Chinese herbal medicine leaching liquor after lixiviate
4.7H
2o, 0.01%CaCl
2, pH is adjusted to 7.0～7.2;
(4) aerobic fermentation: select 50L liquid fermentation tank, liquid amount is 60～70%, inoculation bacillus subtilis and S. cervisiae, inoculum concentration is respectively 2～4%, 5～8%, 28～37 DEG C of controlled fermentation temperature, rotating speed 150～300rpm, throughput 10～20L/min; At this fermentation condition bottom fermentation 12～24h;
(5) anaerobic fermentation: after aerobic fermentation finishes, inoculation lactobacillus bulgaricus and bifidobacterium bifidum, inoculum concentration is 2～4%, 28～37 DEG C of fermentation temperatures, temperature control anaerobic fermentation 12～24h;
(6) fermentation post processing: after having fermented, fermented liquid is adsorbed with 1.2～2 volume ratio wheat brans, dry in 40～60 DEG C of blast driers, moisture is controlled at 8～10%;
(7) pulverize: by the pulverizing of sieving of dried product pulverizer 60 orders, obtain spot trout salmon fermented type Chinese medicine immunity enhancer.
The invention provides a kind of spot trout salmon Chinese medicine immunity enhancer, have prevention, health care, treatment and regulate the multiple efficacies such as immunity, is ideal, green, environmental protection, safety, the novel fodder additive of substitute antibiotics medicine.This invention fermentation of Chinese herbal medicine feed addictive one is efficiently to nurse one's health spot trout salmon intestinal health, treats both principal and secondary aspect of disease, and reinvigoration, keeps intestinal microecology balance and best immune state, improves the digestibility of nutriment, improves animal body immunity; The 2nd, can suppress and stop the generation of the harmful bacterias such as cause of disease coliform, clostridium, salmonella, increase beneficial bacteria of intestinal tract, maintain healthy gut flora; The 3rd, strengthen immunization, be cell by stimulating immunity in enteron aisle, increase the formation of local antibody, increase macrophage activity; The 4th, fermentation of Chinese herbal medicine feed addictive enters in intestines and resists harmful bacteria, reduces stench and harmful components and forms, and makes the ight soil of discharging eliminate stench, in protection breeding environment, improves spot trout salmon meat quality.
The invention provides a kind of spot trout salmon Chinese medicine immunity enhancer, described spot trout salmon is 0.1～0.5% with the mass ratio that fermented type Chinese medicine immunity enhancer product uses in aquatic feeds complete diet pellet: or described feed addictive premix, concentrate feed in complete diet pellet required ratio add and use.
The invention provides a kind of spot trout salmon Chinese medicine immunity enhancer, it is advantageous that: the one, in fermentation of Chinese herbal medicine feed addictive preparation process, adopt the production technology of " Chinese herbal medicine Ultrasonic Wave-Assisted Extraction+many bacterial classifications two-step fermentation ", action effect is more remarkable compared with existing Chinese herbal feed additive product; The 2nd, fermentation of Chinese herbal medicine feed addictive has the two-fold advantage of Chinese herbal medicine and probio, can efficiently nurse one's health spot trout salmon intestinal health, treat both principal and secondary aspect of disease, reinvigoration, keep intestinal microecology balance and best immune state, improve the digestibility of nutriment, improve animal body immunity, improve cultivation income, reduce cultivation risk; The 3rd, the alternative antibiotic of this product uses, and spot trout salmon is had no adverse effects, and uses safety, does not have the problem such as medicament residue and drug resistance, has wide market prospects.
Detailed description of the invention
embodiment 1:
A kind of spot trout salmon Chinese medicine immunity enhancer, concrete preparation process is as follows:
(1) weigh: accurately weigh Chinese herbal medicine according to following formula: 12 parts of gynostemma pentaphylla, 12 parts of Huang Ling, 8 parts of hawthorn, 10 parts of Radix Isatidis, 3 parts of polygala roots, 10 parts of cordate houttuynias, 6 parts of the barks of eucommia, 5 parts, Radix Glycyrrhizae, old 6 parts of bacterium, 10 parts of the seed of Chinese dodders, 6 parts, cactus, 7 parts of glossy ganodermas, 3 parts of barrenwort;
(2) preparation of compound Chinese herb: the Chinese herbal medicine powder after weighing is broken to 40～60 orders, mixes, make compound Chinese herb;
(3) Ultrasonic Wave-Assisted Extraction: to the water that adds 5 times in compound Chinese herb pulvis, under ultrasonic wave booster action, lixiviate 20～30min refluxes under 100 DEG C of conditions;
(4) zymotic fluid preparation: add 3.0% soluble starch, 3.0% beancake powder, 0.3% potassium dihydrogen phosphate, 0.02%MgSO in the Chinese herbal medicine leaching liquor after lixiviate
4.7H
2o, 0.01%CaCl
2, pH is adjusted to 7.0～7.2;
(5) aerobic fermentation: select 50L liquid fermentation tank, liquid amount is 60～70%, inoculation bacillus subtilis and S. cervisiae, inoculum concentration is respectively 2%, 8%, 28～37 DEG C of controlled fermentation temperature, rotating speed 150～300rpm, throughput 10～20L/min; At this fermentation condition bottom fermentation 12h;
(6) anaerobic fermentation: after aerobic fermentation finishes, inoculation lactobacillus bulgaricus and bifidobacterium bifidum, inoculum concentration is respectively 2%, 4%, 28～37 DEG C of fermentation temperatures, temperature control anaerobic fermentation 24h;
(7) fermentation post processing: after having fermented, fermented liquid is adsorbed with 1.2 times of volume ratio wheat brans, dry in 40～60 DEG C of blast driers, moisture is controlled at 8～10%;
(8) pulverize: by the pulverizing of sieving of dried product pulverizer 60 orders, obtain spot trout salmon fermented type Chinese medicine immunity enhancer.
embodiment 2:
A kind of spot trout salmon Chinese medicine immunity enhancer, concrete preparation process is as follows:
(1) weigh: accurately weigh Chinese herbal medicine according to following formula: 18 parts of gynostemma pentaphylla, 8 parts of Huang Ling, 12 parts of hawthorn, 6 parts of Radix Isatidis, 5 parts of polygala roots, 6 parts of cordate houttuynias, 8 parts of the barks of eucommia, 3 parts, Radix Glycyrrhizae, old 10 parts of bacterium, 6 parts of the seed of Chinese dodders, 10 parts, cactus, 5 parts of glossy ganodermas, 5 parts of barrenwort;
(2) preparation of compound Chinese herb: the Chinese herbal medicine powder after weighing is broken to 40～60 orders, mixes, make compound Chinese herb;
(3) Ultrasonic Wave-Assisted Extraction: to the water that adds 10 times in compound Chinese herb pulvis, under ultrasonic wave booster action, lixiviate 20～30min refluxes under 100 DEG C of conditions;
(4) zymotic fluid preparation: add 5.0% soluble starch, 2.0% beancake powder, 0.5% potassium dihydrogen phosphate, 0.02%MgSO in the Chinese herbal medicine leaching liquor after lixiviate
4.7H
2o, 0.01%CaCl
2, pH is adjusted to 7.0～7.2;
(5) aerobic fermentation: select 50L liquid fermentation tank, liquid amount is 60～70%, inoculation bacillus subtilis and S. cervisiae, inoculum concentration is respectively 4%, 5%, 28～37 DEG C of controlled fermentation temperature, rotating speed 150～300rpm, throughput 10～20L/min; At this fermentation condition bottom fermentation 24h;
(6) anaerobic fermentation: after aerobic fermentation finishes, inoculation lactobacillus bulgaricus and bifidobacterium bifidum, inoculum concentration is respectively 4%, 2%, 28～37 DEG C of fermentation temperatures, temperature control anaerobic fermentation 12h;
(7) fermentation post processing: after having fermented, fermented liquid is adsorbed with 2 times of volume ratio wheat brans, dry in 40～60 DEG C of blast driers, moisture is controlled at 8～10%;
(8) pulverize: by the pulverizing of sieving of dried product pulverizer 60 orders, obtain spot trout salmon fermented type Chinese medicine immunity enhancer.
embodiment 3:
A kind of spot trout salmon Chinese medicine immunity enhancer, concrete preparation process is as follows:
(1) weigh: accurately weigh Chinese herbal medicine according to following formula: 15 parts of gynostemma pentaphylla, 10 parts of Huang Ling, 10 parts of hawthorn, 8 parts of Radix Isatidis, 4 parts of polygala roots, 8 parts of cordate houttuynias, 7 parts of the barks of eucommia, 4 parts, Radix Glycyrrhizae, old 8 parts of bacterium, 8 parts of the seed of Chinese dodders, 8 parts, cactus, 6 parts of glossy ganodermas, 4 parts of barrenwort;
(2) preparation of compound Chinese herb: the Chinese herbal medicine powder after weighing is broken to 40～60 orders, mixes, make compound Chinese herb;
(3) Ultrasonic Wave-Assisted Extraction: to the water that adds 7.5 times in compound Chinese herb pulvis, under ultrasonic wave booster action, lixiviate 20～30min refluxes under 100 DEG C of conditions;
(4) zymotic fluid preparation: add 4.0% soluble starch, 2.5% beancake powder, 0.4% potassium dihydrogen phosphate, 0.02%MgSO in the Chinese herbal medicine leaching liquor after lixiviate
4.7H
2o, 0.01%CaCl
2, pH is adjusted to 7.0～7.2;
(5) aerobic fermentation: select 50L liquid fermentation tank, liquid amount is 60～70%, inoculation bacillus subtilis and S. cervisiae, inoculum concentration is respectively 3%, 6.5%, 28～37 DEG C of controlled fermentation temperature, rotating speed 150～300rpm, throughput 10～20L/min; At this fermentation condition bottom fermentation 18h;
(6) anaerobic fermentation: after aerobic fermentation finishes, inoculation lactobacillus bulgaricus and bifidobacterium bifidum, inoculum concentration is 2～4%, 28～37 DEG C of fermentation temperatures, temperature control anaerobic fermentation 18h;
(7) fermentation post processing: after having fermented, fermented liquid is adsorbed with 1.6 times of volume ratio wheat brans, dry in 40～60 DEG C of blast driers, moisture is controlled at 8～10%;
(8) pulverize: by the pulverizing of sieving of dried product pulverizer 60 orders, obtain spot trout salmon fermented type Chinese medicine immunity enhancer.
Above embodiment is only for technical scheme of the present invention is described, but not is limited; Although with reference to previous embodiment to being had been described in detail by invention, for the person of ordinary skill of the art, the technical scheme that still can record previous embodiment is modified, or part technical characterictic is wherein equal to replacement; And to these amendments or replacement, do not make the essence of appropriate technical solution depart from the spirit and scope of the present invention's technical scheme required for protection.
Claims (1)
1. a preparation method for fermented type Chinese medicine immunity enhancer for spot trout salmon, is characterized in that adopting " two-step fermentation that Chinese herbal medicine Ultrasonic Wave-Assisted Extraction+many bacterial classifications are aerobic and anaerobic fermentation combines " technique; Specifically comprise the following steps:
(1) preparation of compound Chinese herb: accurately weigh herbal raw material in 12～18 parts of gynostemma pentaphylla, 8～12 parts of Huang Ling, 8～12 parts of hawthorn, 6～10 parts of Radix Isatidis, 3～5 parts of polygala roots, 6～10 parts of cordate houttuynias, 6～8 parts of the barks of eucommia, 3～5 parts, Radix Glycyrrhizae, old 6～10 parts of bacterium, 6～10 parts of the seed of Chinese dodders, 6～10 parts, cactus, 5～7 parts of glossy ganodermas, 3～5 parts of ratios of barrenwort, be crushed to 40～60 orders, mix, make compound Chinese herb;
(2) Ultrasonic Wave-Assisted Extraction: to the water that adds 5～10 times in compound Chinese herb pulvis, under ultrasonic wave booster action, lixiviate 20～30min refluxes under 100 DEG C of conditions;
(3) zymotic fluid preparation: add 3.0～5.0% soluble starches, 2.0～3.0% beancake powders, 0.3～0.5% potassium dihydrogen phosphate, 0.02%MgSO in the Chinese herbal medicine leaching liquor after lixiviate
4.7H
2o, 0.01%CaCl
2, pH is adjusted to 7.0～7.2;
(4) aerobic fermentation: select 50L liquid fermentation tank, liquid amount is 60～70%, inoculation bacillus subtilis and S. cervisiae, inoculum concentration is respectively 2～4%, 5～8%, 28～37 DEG C of controlled fermentation temperature, rotating speed 150～300rpm, throughput 10～20L/min; At this fermentation condition bottom fermentation 12～24h;
(5) anaerobic fermentation: after aerobic fermentation finishes, inoculation lactobacillus bulgaricus and bifidobacterium bifidum, inoculum concentration is 2～4%, 28～37 DEG C of fermentation temperatures, temperature control anaerobic fermentation 12～24h;
(6) fermentation post processing: after having fermented, fermented liquid is adsorbed with 1.2～2 volume ratio wheat brans, dry in 40～60 DEG C of blast driers, moisture is controlled at 8～10%;
(7) pulverize: by the pulverizing of sieving of dried product pulverizer 60 orders, obtain spot trout salmon fermented type Chinese medicine immunity enhancer.
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| CN104222674B (en) * | 2014-09-30 | 2016-06-08 | 青岛嘉瑞生物技术有限公司 | The preparation method of a kind of channel catfish Chinese medicine immunity enhancer and application |
| CN104187436A (en) * | 2014-09-30 | 2014-12-10 | 青岛嘉瑞生物技术有限公司 | Preparation method of meat rabbit feed additive obtained through fermentation of seaweed and Chinese herbal medicines |
| CN104304748A (en) * | 2014-10-10 | 2015-01-28 | 青岛嘉瑞生物技术有限公司 | Probiotic fermented feed additive for dairy cattle |
| CN105132310B (en) * | 2015-08-07 | 2019-09-03 | 哈尔滨动物生物制品国家工程研究中心有限公司 | One plant of cold water fish probiotics Bacillus strain and application thereof |
| CN105192398A (en) * | 2015-10-15 | 2015-12-30 | 无锡市南阳湖水产科技有限公司 | Salmon aquatic product aquaculture feed |
| CN106071405A (en) * | 2016-06-27 | 2016-11-09 | 青岛铭晨信息技术有限公司 | A kind of preparation method and application of Penaeus vannamei Chinese medicine immunity enhancer |
| CN107469026A (en) * | 2017-07-20 | 2017-12-15 | 通化师范学院 | A kind of Chinese medicine immunity enhancer for strengthening salmon para-immunity power and application thereof |
| CN107412537A (en) * | 2017-07-20 | 2017-12-01 | 通化师范学院 | A kind of Chinese herbal feed additive that can strengthen salmon immunological regulation suppression |
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| CN100581378C (en) * | 2006-08-01 | 2010-01-20 | 济南亿民动物药业有限公司 | Method for preparing fermented Chinese herbal medicine preparations for feed |
| CN101332226A (en) * | 2007-06-26 | 2008-12-31 | 肖志东 | Compound toxin expelling and antioxidation biology oral liquid and preparation method thereof |
| CN101791054A (en) * | 2010-04-28 | 2010-08-04 | 中国水产科学研究院黑龙江水产研究所 | Game fish Chinese herbal medicine complex additive |
| CN103583810B (en) * | 2013-11-26 | 2015-05-13 | 青岛嘉瑞生物技术有限公司 | Fermentation type Chinese herbal medicinal immunopotentiator for erythroculter ilishaeformis |
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