CN103599354B - A kind of pine pollen composition and application thereof - Google Patents
A kind of pine pollen composition and application thereof Download PDFInfo
- Publication number
- CN103599354B CN103599354B CN201310573472.1A CN201310573472A CN103599354B CN 103599354 B CN103599354 B CN 103599354B CN 201310573472 A CN201310573472 A CN 201310573472A CN 103599354 B CN103599354 B CN 103599354B
- Authority
- CN
- China
- Prior art keywords
- extract
- radix
- filtrate
- pollen pini
- water
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Landscapes
- Medicines Containing Plant Substances (AREA)
Abstract
The present invention relates to a kind of pine pollen composition, be made up of the raw material of following weight portion: breaking cellular wall Pollen Pini 20～40 parts, Radix Rhodiolae extract 10～30 parts, Radix Ginseng extract 5～15 parts, Radix Ophiopogonis extract 1～5 part；The present composition can increase muscle power, allaying tiredness, raising anoxia endurance, for preparing medicine or the health product of alleviating physical fatigue.
Description
Technical field
The present invention relates to a kind of pine pollen composition and application thereof, belong to nutrition, health food and field of medicaments.
Background technology
Pollen Pini medicine name pine is yellow, for the pollen of pinaceae plant Pinus massoniana Lamb, Pinus tabuliformis or its congener, plucks spica and dries collection, remove the faint yellow thin pollen of impurity gained when spring is spent and just opens.Its nature and flavor are sweet flat nontoxic, Pollen Pini contains plants nutritional labeling up to more than 200, including most vitamin, aminoacid, enzyme, wherein every kind of component content is all bigger, many 7～10 times of the Pollen Pini total protein relatively beef of equivalent weight, egg, mineral ferrum is higher 20 times than Herba Spinaciae content, and vitamin A prototype carotene wants many 20～30 times, it more difficult to what obtain is that more than 200 kind nutritional labelings contained by Pollen Pini are all active.Just having the record of " pine Huang " in first pharmacopeia Shennong's Herbal of China, its function is: " pine is yellow, and abnormal smells from the patient is sweet flat nontoxic, cures mainly trusted subordinate's cold and heat pathogen, diuresis, and disappear congestion, takes physical strength profiting of making light of one's life by commiting suicide for a long time, prolongs life." be classified as top grade.Pollen Pini is now widely used in the fields such as health food, medicine, cosmetics.Correlational study shows, containing the nutrition of equilibrium comprehensively in Pollen Pini, it is possible to increases muscle power and energy, makes fatigue be recovered comprehensively.
The applicating history of Radix Rhodiolae is long, and before more than 2,000 year, the people of Qinghai-Tibet Platean is just used as medicine with it, carrys out building body with this.Among the people being commonly used to is steep in wine, and can banish fatigue, and opposing mountain area is cold, and also diseases prevention is healthy and strong simultaneously, and nourishing lengthens one's life.Radix Rhodiolae can improve rapidly the binding ability of hemoglobin and oxygen, improves blood oxygen saturation, reduces the oxygen consumption of body, increases exercise tolerance, recovers Fatique after Sports, therefore has the effect of anti-hypoxia, resisting fatigue.Radix Rhodiolae, possibly together with compositions such as a large amount of scopolamine, kaempferols, has the effect of anti-inflammation；Content of tannin is also a lot, can shield at mucomembranous surface, prevents too much secretion and excessive hemorrhage, and cough, expectoration and women's leucorrhoea grow in quantity that pneumonia is caused are effective in cure.Radix Rhodiolae can substantially suppress the radiation-induced heart and liver LPO(lipid peroxide) generation, lipid and cell membrane are played a protective role, play radiation resistance；The activity of body SOD, scavenging free radicals can also be significantly improved, it is suppressed that lipid peroxide generates, slow down aging.
Radix Ginseng is called " king of BAICAO " by people, is one of well-known " northeast three essentials-essence ", be have won fame both at home and abroad, rare medicinal herbs that old children all knows.Why very rare Radix Ginseng is, very famous and precious, main relevant with its medical value.Medical book Shennong's Herbal very early is considered as, Radix Ginseng have " tonifying five ZANG-organs, peace spirit, determine soul, spasmolytic is throbbed with fear, evil removing gas, the happy Fructus Alpiniae Oxyphyllae of improving eyesight " effect, " take for a long time make light of one's life by commiting suicide prolong life ".Radix Ginseng is also extremely praised highly by Li Shizhen (1518-1593 A.D.) in Compendium of Material Medica, it is believed that it " can control man's all deficient syndromes of woman ".For thousands of years, in Chinese herbal medicine, Radix Ginseng is all listed in " top grade ".The active component of Radix Ginseng is mainly ginsenoside, and research shows that the antifatigue effect of ginsenoside Rg1 is notable, and remaining lipotropic component after isolating ginsenoside also can increase the autonomic movement of mice, show antifatigue effect.Radix Ginseng can improve the availability of glycogen, energy-rich phosphate, it is prevented that the accumulation of lactic acid and acetone acid, and makes its metabolism more complete.Radix Ginseng also can stop in the tissue that rat prolonged exercise causes cholesterol depletion in glycogen and adrenal gland.Radix Ginseng can promote the synthesis of the synthesis of protein, the synthesis of RNA and DNA.Also can change the reactivity of body, similar to Radix Et Caulis Acanthopanacis Senticosi, Radix Schisandrae Bicoloris etc., there is the effect of " adaptogen ", can the enhancing body respond to various destructive stimuluses, strengthen organism adaptation.In addition, cardiovascular system, liver etc. are had good effect by Radix Ginseng.
Radix Ophiopogonis, Liliaceae Ophiopogon perennial evergreen herbaceous plant.Main component is Saponin Radix Ophiopogonis.Radix Ophiopogonis hardship sweet, micro-, cool, Yin-nourishing and body fluid promoting, nourishing the lung to arrest cough, clear away heart-fire relieving restlessness, cures mainly consumption of body fluid caused by febrile disease, vexed, thirsty, dry pharynx lung-heat, cough, pulmonary tuberculosis Radix Ophiopogonis.Central nervous system and cardiovascular system are had good effect by Radix Ophiopogonis, and research shows, fibrous root of Radix Ophiopogonis extract is improved the effect of cellular immunization.Saponin contained by Radix Ophiopogonis, polysaccharide, aminoacid etc. have obvious antifatigue effect.
Fatigue is a kind of physiological phenomenon, is a kind of protective mechanism for people.This is that health sends the signal of this rest to us, if no matter ignored, health will suffer damage.It is tired that fatigue generally can be divided into physical fatigue, mental fatigue, psychological fatigue and pathology.
Physical fatigue is again body tiredness, when being the physical exertion campaign due to, big intensity long-time as people, internal create substantial amounts of metabolite, such as lactic acid, carbon dioxide, serum urea nitrogen etc., this kind of material gathers in vivo, stimulate human tissue cell and nervous system, people will be made to produce tired firm.For the development epoch of current two-forty, the body and mind of people is easier to feel tired, is no matter the fatigue of the fatigue of muscle power or psychology, and people easily show different shapes, such as the agitation of temper, the reduction of body immunity.Then, the medicine of alleviating physical fatigue causes the concern of more and more scholar.
Research shows, Pollen Pini just can be conducive to its effective ingredient of absorption of human body after only having breaking cellular wall, and very easily oxidation and microbiological contamination after breaking cellular wall, it is noted that the preservation of Pollen Pini and utilization after breaking cellular wall.
Summary of the invention
The technical problem to be solved is to provide a kind of pine pollen composition and application thereof, and the present composition can increase muscle power, allaying tiredness, raising anoxia endurance, for preparing the medicine of alleviating physical fatigue.
The technical scheme is that a kind of pine pollen composition, be made up of the raw material of following weight portion:
Wherein, breaking cellular wall Pollen Pini prepares by the following method:
Take Pollen Pini raw material, through the hot air drying of 80～100 DEG C, the temperature of dry mixed room is 60～80 DEG C, dry 5～60s, dried Pollen Pini crosses the sieve of 80～200 orders, direct plunge in grading sieving machine, grading wheel scavenging air pressure is 0.2～0.4MPa, motor crankshaft scavenging air pressure is 0.05～0.1MPa, catcher pulse of air pressure 0.6MPa, Pollen Pini after classification is put in air-flow wall-broken grinder, the frequency of the classification motor that air-flow processes is 50～60Hz, bleed pressure is 0.5～1.5MPa, discharging opening temperature is maintained at 5～15 DEG C, breaking cellular wall Pollen Pini sterilizing bag completely is packed, standby；
Wherein, Radix Rhodiolae extract prepares by the following method:
Weigh Radix Rhodiolae, be 0.1～0.15MPa at pressure, when temperature is 65～85 DEG C, by water reflux, extract, 3 times, each amount of water is 8 times of Radix Rhodiolae quality, extracts 3 hours every time, extracting solution, with 80～100 mesh screen, must extract filtrate, merges the extraction filtrate of three gained, extract filtrate concentrate drying, obtain dry powder, pulverize, cross 80～200 mesh sieves, obtain Radix Rhodiolae extract
Wherein, Radix Ginseng extract prepares by the following method:
Weigh Radix Ginseng, be 0.1～0.15MPa at pressure, when temperature is 65～85 DEG C, by water reflux, extract, 3 times, each amount of water is 8 times of Ginseng Quality, extracts 3 hours every time, extracting solution, with 80～100 mesh screen, must extract filtrate, merges the extraction filtrate of three gained, extract filtrate concentrate drying, obtain dry powder, pulverize, cross 80～200 mesh sieves, obtain Radix Ginseng extract
Wherein, Radix Ophiopogonis extract prepares by the following method:
Weigh Radix Ophiopogonis, be 0.1～0.15MPa at pressure, when temperature is 65～85 DEG C, by water reflux, extract, 2 times, each amount of water is Radix Ophiopogonis 8 times of quality, extracts 3 hours every time, extracting solution is with 80～100 mesh screen, filtrate must be extracted, merge the extraction filtrate of secondary gained, extract filtrate concentrate drying, obtain dry powder, pulverize, cross 80～200 mesh sieves, obtain Radix Ophiopogonis extract.
On the basis of technique scheme, the present invention can also do following improvement.
Further, the particle diameter of described breaking cellular wall Pollen Pini is 3～5 μm.
Further, described pine pollen composition is made up of the raw material of following weight portion:
Further, said composition adds one or more excipient pharmaceutically accepted or adjuvant makes capsule, tablet, pill or granule.
Further, described solid excipient or adjuvant are one or both the mixture in microcrystalline Cellulose, Sorbitol.
Present invention also offers the application in the medicine preparing alleviating physical fatigue and health product of the above-mentioned pine pollen composition.
On the basis of technique scheme, the present invention can also do following improvement.
Further, described alleviating physical fatigue includes increasing muscle power, allaying tiredness, raising anoxia endurance.
The invention has the beneficial effects as follows:
The mitigation effect that the physical fatigue that the physical exertion campaign of intensity long-time, big causes is shown by pine pollen composition of the present invention from the compositions of breaking cellular wall Pollen Pini, Radix Rhodiolae extract, Radix Ginseng extract and Radix Ophiopogonis extract is notable.
Contained by pine pollen composition of the present invention, the percentage by weight of main active substances is as follows: ginsenoside content >=0.5%.
The content of above-mentioned principle active component measures as follows:
Accurately weighed compositions 1.00g, add methanol 25mL, close plug, shake up, weigh, room temperature merceration 24h, ultrasonic Treatment 10min, and complement to former weighed weight with methanol, centrifugation 10min, accurate Aspirate supernatant 2mL, fling to methanol, residue 2mL water dissolution also moves into the D101 macroporous adsorptive resins handled well, first wash (flow velocity 0.4～0.5mL/min) with water 15mL, discard water lotion, continue with 70% ethanol elution, collect eluent 25mL, volatilize in water-bath, residue methanol dissolves, filter, filtrate is put in 2mL measuring bottle, with methanol dilution to scale, shake up, as need testing solution.With panaxatriol for reference substance, accurately weighed in right amount, add methanol and be configured to the reference substance solution of 1mg/mL.
Accurate absorption reference substance solution 10,20,40,60,80,100 μ L, it is respectively placed in 10mL tool plug test tube, fling to solvent, add 5% vanillin glacial acetic acid solution 0.2mL of new preparation, perchloric acid 0.8mL, put after 60 DEG C of water-baths are incubated 15min, 5min is cooled down immediately with frozen water, add glacial acetic acid 5mL, shake up, place l0min, make reference with reagent blank, according to ultraviolet visible spectrophotometry (Pharmacopoeia of the People's Republic of China one annex VA of version in 2010), measure trap immediately at 560nm wavelength place.With concentration for abscissa, with trap for vertical coordinate, drawing standard curve.
Accurate absorption need testing solution 0.2mL, put in 10mL tool plug test tube, solvent is flung in water-bath, under sighting target directrix curve preparation, method is from " adding 5% vanillin glacial acetic acid solution 0.2mL of new preparation ", pressing maneuver measures trap, from standard curve read or regression equation calculate to obtain the weight (μ g) of ginsenoside, then calculate the content of Radix Ginseng total saponins in sample.
Detailed description of the invention
Hereinafter principles of the invention and feature being described, example is served only for explaining the present invention, is not intended to limit the scope of the present invention.
Embodiment 1
The preparation method of breaking cellular wall Pollen Pini: take Pollen Pini raw material, through the hot air drying of 100 DEG C, the temperature of dry mixed room is 60 DEG C, dry 10s, dried Pollen Pini crosses the sieve of 80 orders, direct plunge in grading sieving machine, grading wheel scavenging air pressure is 0.4MPa, motor crankshaft scavenging air pressure is 0.1MPa, catcher pulse of air pressure 0.6MPa, Pollen Pini after classification is put in air-flow wall-broken grinder, the frequency of the classification motor that air-flow processes is 60Hz, bleed pressure is 1.5MPa, discharging opening temperature is maintained at 15 DEG C, breaking cellular wall Pollen Pini sterilizing bag completely is packed, standby；
Weighing Radix Rhodiolae, be 0.15MPa at pressure, when temperature 85 DEG C, by water reflux, extract, 3 times, each amount of water is 8 times of Radix Rhodiolae quality, extracts 3 hours every time, and extracting solution crosses 100 mesh screen, must extract filtrate, the extraction filtrate of three gained of merging；Extract filtrate concentrate drying, obtain dry powder, pulverize, cross 200 mesh sieves, obtain Radix Rhodiolae extract.
Weighing Radix Ginseng, be 0.15MPa at pressure, when temperature 65 DEG C, by water reflux, extract, 3 times, each amount of water is 8 times of Ginseng Quality, extracts 3 hours every time, and extracting solution crosses 100 mesh screen, must extract filtrate, the extraction filtrate of three gained of merging；Extract filtrate concentrate drying, obtain dry powder, pulverize, cross 200 mesh sieves, obtain Radix Ginseng extract.
Weighing Radix Ophiopogonis, be 0.15MPa at pressure, when temperature 85 DEG C, by water reflux, extract, 2 times, each amount of water is Radix Ophiopogonis 8 times of quality, extracts 3 hours every time, and extracting solution crosses 80 mesh screen, must extract filtrate, the extraction filtrate of merging secondary gained；Extract filtrate concentrate drying, obtain dry powder, pulverize, cross 100 mesh sieves, obtain Radix Ophiopogonis extract.
Weigh breaking cellular wall Pollen Pini 260g, Radix Ginseng extract 100g, Radix Rhodiolae extract 160g, Radix Ophiopogonis extract 40g mixes, and crosses 80 mesh sieves, adds microcrystalline Cellulose 300g and forms pharmaceutical formulation, mixing, spraying into 50% Sorbitol 280g is binding agent soft material, granulates with 24 mesh sieves, dried granulate, mixing, tabletting (tablet weight 0.5g), coating, obtaining, composition tablet product is containing Radix Ginseng total saponins >=500mg/100g.
Embodiment 2
Take Pollen Pini raw material, through the hot air drying of 95 DEG C, the temperature of dry mixed room is 80 DEG C, dry 25s, dried Pollen Pini crosses the sieve of 80 orders, direct plunge in grading sieving machine, grading wheel scavenging air pressure is 0.4MPa, and motor crankshaft scavenging air pressure is 0.1MPa, catcher pulse of air pressure 0.6MPa, Pollen Pini after classification is put in air-flow wall-broken grinder, the frequency of the classification motor that air-flow processes is 50Hz, and bleed pressure is 1.0MPa, and discharging opening temperature is maintained at 10 DEG C, breaking cellular wall Pollen Pini sterilizing bag completely is packed, standby；
Weighing Radix Rhodiolae, be 0.1MPa at pressure, when temperature 85 DEG C, by water reflux, extract, 3 times, each amount of water is 8 times of Radix Rhodiolae quality, extracts 3 hours, extracting solution 90 mesh screen every time, must extract filtrate, the extraction filtrate of three gained of merging；Extract filtrate concentrate drying, obtain dry powder, pulverize, cross 80 mesh sieves, obtain Radix Rhodiolae extract.
Weighing Radix Ginseng, be 0.1MPa at pressure, when temperature 75 DEG C, by water reflux, extract, 3 times, each amount of water is 8 times of Ginseng Quality, extracts 3 hours every time, and extracting solution crosses 90 mesh screen, must extract filtrate, the extraction filtrate of three gained of merging；Extract filtrate concentrate drying, obtain dry powder, pulverize, cross 100 mesh sieves, obtain Radix Ginseng extract.
Weighing Radix Ophiopogonis, be 0.1MPa at pressure, when temperature 75 DEG C, by water reflux, extract, 2 times, each amount of water is Radix Ophiopogonis 8 times of quality, extracts 3 hours every time, and extracting solution 90 order filters, and must extract filtrate, the extraction filtrate of merging secondary gained；Extract filtrate concentrate drying, obtain dry powder, pulverize, cross 80 mesh sieves, obtain Radix Ophiopogonis extract.
Weigh breaking cellular wall Pollen Pini 290g, Radix Ginseng extract 80g, Radix Rhodiolae extract 170g, Radix Ophiopogonis extract 420g mixes, and crosses 80 mesh sieves, adds microcrystalline Cellulose 300g and forms pharmaceutical formulation, mixing, spraying into 50% Sorbitol 280g is binding agent soft material, granulates with 24 mesh sieves, dried granulate, mixing, tabletting (tablet weight 0.5g), coating, obtaining, composition tablet product is containing Radix Ginseng total saponins >=550mg/100g.
Compositions prepared by embodiment 3 present invention impact on mice burden swimming
1, laboratory animal and group are arranged
Take female KM mice 120, adapt to environment and after 3 days, carry out swimming screening test, it is divided into five groups at random, often 15 mices of group, first group is blank group, in second group, (BW is writing a Chinese character in simplified form of bodyweight to the tablet 1g/kgBW of every mouse feeding embodiment 1 preparation, refer to Mouse Weight), the tablet 0.5g/kgBW of every mouse feeding embodiment 1 preparation in 3rd group, the tablet 1g/kgBW of every mouse feeding embodiment 2 preparation in 4th group, the tablet 0.5g/kgBW of every mouse feeding embodiment 2 preparation in the 5th group.
2, experimental technique
Giving given the test agent to experiment mice gavage, once a day, continuous 30 days, blank group gave CMCNa, every mouse feeding 20ml/kgBW.Weigh in weekly twice, and adjust dosage in good time.After last gives given the test agent 30min, mice is placed in swimming trunk went swimming.Depth of water 30cm, water temperature 25 ± 1.0 DEG C, the sheet lead of rat-tail root load 5% body weight.Record mice starts to the dead time from swimming, as the mice burden swimming time.
3, experimental data statistics
Adopting variance analysis, but need to first carry out homogeneity test of variance by the program of variance analysis, variance is neat, calculates F value, F value < F0.05, conclusion: no significant difference between each group mean；F value >=F0.05, P≤0.05, adds up with the comparative approach between two of mean between multiple experimental grouies and a matched group；The data of abnormal or heterogeneity of variance are carried out suitable variable conversion, after meeting normal state or variance requires together, adds up by the data after conversion；If purpose still neat not up to normal state or variance after variable conversion, use rank test instead and add up.Adopt variance analysis to add Q inspection to add up.
4, result and analysis
4th group, the 5th group can significantly extend heavy burden mice swimming time, compare with blank group, has significant difference (P < 0.01).In Table 1.
The impact on the mice burden swimming time of table 1 compositionsn=15)
Note: compare with first group (blank group): * P < 0.05, * * P < 0.01, * * * P < 0.001.
Pine pollen composition prepared by embodiment 4 present invention is on the impact of serum urea content in Mice Body
1, laboratory animal and group are arranged
Take male BALB/c mouse 100, adapt to environment 3 days, it is divided into seven groups at random, often 12 mices of group, first group is blank group, the tablet 1g/kgBW of every mouse feeding embodiment 1 preparation in second group, the tablet 0.5g/kgBW of every mouse feeding embodiment 1 preparation in 3rd group, the tablet 0.25g/kgBW of every mouse feeding embodiment 1 preparation in 4th group, the tablet 1g/kgBW of every mouse feeding embodiment 2 preparation in 5th group, the tablet 0.5g/kgBW of every mouse feeding embodiment 2 preparation in 6th group, the tablet 0.25g/kgBW of every mouse feeding embodiment 2 preparation in 7th group.
2, experimental technique
Giving given the test agent to experiment mice gavage, once a day, continuous 30 days, blank group gave distilled water, every mouse feeding 20ml/kgBW.Weigh in weekly twice, and adjust dosage in good time.Prepared by foundation and the specimen of high carbamide model: last is to after given the test agent 30min, not swimming with a load attached to the body 90min in the water that temperature is 30 DEG C, after rest 60min, mice is extractd eyeball and adopts whole blood and be about 0.5mL(and be not added with anticoagulant), put 4 DEG C of refrigerators and be about 3h, the centrifugal 15min of 2000prn/min after hemopexis, serum is standby.
3, experimental data statistics
Adopting variance analysis, but need to first carry out homogeneity test of variance by the program of variance analysis, variance is neat, calculates F value, F value < F0.05, conclusion: no significant difference between each group mean；F value >=F0.05, P≤0.05, adds up with the comparative approach between two of mean between multiple experimental grouies and a matched group；The data of abnormal or heterogeneity of variance are carried out suitable variable conversion, after meeting normal state or variance requires together, adds up by the data after conversion；If purpose still neat not up to normal state or variance after variable conversion, use rank test instead and add up.Adopt variance analysis to add Q inspection to add up.
4, result and analysis
The mice serum urea nitrogen content of the 7th group substantially reduces, and compares with blank group, has significant difference.In Table 2.
The impact on mice serum urea nitrogen content of table 2 compositionsn=15)
In sum, pine pollen composition prepared by the present invention can significantly extend heavy burden mice swimming time, reduces mice serum urea nitrogen content, has the function of alleviating physical fatigue.
Pine pollen composition prepared by embodiment 5 present invention and the impact on mice burden swimming of other compositionss
1, laboratory animal and group are arranged
Take female KM mice 120, adapt to environment and after 3 days, carry out swimming screening test, it is divided into four groups at random, often 15 mices of group, first group is blank group, the tablet 1g/kgBW, the compositions 1g/kgBW of every mouse feeding Radix Ginseng extract and Radix Rhodiolae extract in the 3rd group, every mouse feeding breaking cellular wall Pollen Pini 1g/kgBW in the 4th group of every mouse feeding embodiment 2 preparation in second group.
Wherein, the preparation method of breaking cellular wall Pollen Pini: take Pollen Pini raw material, through the hot air drying of 100 DEG C, the temperature of dry mixed room is 60 DEG C, dry 10s, dried Pollen Pini crosses the sieve of 80 orders, direct plunge in grading sieving machine, grading wheel scavenging air pressure is 0.4MPa, motor crankshaft scavenging air pressure is 0.1MPa, catcher pulse of air pressure 0.6MPa, Pollen Pini after classification is put in air-flow wall-broken grinder, the frequency of the classification motor that air-flow processes is 60Hz, bleed pressure is 1.5MPa, discharging opening temperature is maintained at 15 DEG C, breaking cellular wall Pollen Pini sterilizing bag completely is packed, standby；
Weighing Radix Rhodiolae, be 0.15MPa at pressure, when temperature 85 DEG C, by water reflux, extract, 3 times, each amount of water is 8 times of Radix Rhodiolae quality, extracts 3 hours every time, and extracting solution crosses 100 mesh screen, must extract filtrate, the extraction filtrate of three gained of merging；Extract filtrate concentrate drying, obtain dry powder, pulverize, cross 200 mesh sieves, obtain Radix Rhodiolae extract.Weighing Radix Ginseng, be 0.15MPa at pressure, when temperature 65 DEG C, by water reflux, extract, 3 times, each amount of water is 8 times of Ginseng Quality, extracts 3 hours every time, and extracting solution crosses 100 mesh screen, must extract filtrate, the extraction filtrate of three gained of merging；Extract filtrate concentrate drying, obtain dry powder, pulverize, cross 200 mesh sieves, obtain Radix Ginseng extract.Radix Ginseng extract and Radix Rhodiolae extract are mixed according to mass ratio 1:1, obtains the 3rd group of experiment compositions.
2, experimental technique
Giving given the test agent to experiment mice gavage, once a day, continuous 30 days, blank group gave CMCNa, and volume is 20ml/kgBW.Weigh in weekly twice, and adjust dosage in good time.After last gives given the test agent 30min, mice is placed in swimming trunk went swimming.Depth of water 30cm, water temperature 25 ± 1.0 DEG C, the sheet lead of rat-tail root load 5% body weight.Record mice starts to the dead time from swimming, as the mice burden swimming time.
3, experimental data statistics
Adopting variance analysis, but need to first carry out homogeneity test of variance by the program of variance analysis, variance is neat, calculates F value, F value < F0.05, conclusion: no significant difference between each group mean；F value >=F0.05, P≤0.05, adds up with the comparative approach between two of mean between multiple experimental grouies and a matched group；The data of abnormal or heterogeneity of variance are carried out suitable variable conversion, after meeting normal state or variance requires together, adds up by the data after conversion；If purpose still neat not up to normal state or variance after variable conversion, use rank test instead and add up.Adopt variance analysis to add Q inspection to add up.
4, result and analysis
Second group can significantly extend heavy burden mice swimming time, compare with blank group, has significant difference (P < 0.01)；And it is substantially better than the 3rd group and the 4th group, thus illustrate that the formulation efficacy after combination is more excellent.In Table 3.
The impact on the mice burden swimming time of table 3 compositionsn=15)
| Group | Dosage (g/kgBW) | Walking weight load (min) |
| First group | 87.2±21.4 | |
| Second group | 1.0 | 137.2±48.2** |
| 3rd group | 1.0 | 125.3±33.5* |
| 4th group | 1.0 | 98±29.7 |
Note: compare with first group (blank group): * P < 0.05, * * P < 0.01, * * * P < 0.001.
Existing Radix Ginseng extract or Radix Rhodiolae extract anti-fatigue product are taken and are had the phenomenon got angry in process, Pollen Pini prescription prepared by the present invention is according to theory of Chinese medical science, add Radix Ophiopogonis in the composition, " property of medicine opinion " is recorded Radix Ophiopogonis and " controls pyretic toxicity; only excessive thirst; main flood appearance podomere edema, lower water." supplement to the Herbal is recorded and " controls cold and heat body labor, lower phlegm retention Radix Ophiopogonis." amplification on Canon of Materia Medica is recorded and " controls cardiopulmonary deficiency-heat Radix Ophiopogonis." visible, have Radix Ophiopogonis and have the relieving restlessness that clears away heart-fire, solve dysphoric effect, can effectively reduce the scorching of Radix Ginseng and Radix Rhodiolae, after taking, not have the phenomenon got angry.
The foregoing is only presently preferred embodiments of the present invention, not in order to limit the present invention, all within the spirit and principles in the present invention, any amendment of making, equivalent replacement, improvement etc., should be included within protection scope of the present invention.
Claims (1)
1. a pine pollen composition, it is characterised in that
Take Pollen Pini raw material, through the hot air drying of 95 DEG C, the temperature of dry mixed room is 80 DEG C, dry 25s, dried Pollen Pini crosses the sieve of 80 orders, direct plunge in grading sieving machine, grading wheel scavenging air pressure is 0.4MPa, and motor crankshaft scavenging air pressure is 0.1MPa, catcher pulse of air pressure 0.6MPa, Pollen Pini after classification is put in air-flow wall-broken grinder, the frequency of the classification motor that air-flow processes is 50Hz, and bleed pressure is 1.0MPa, and discharging opening temperature is maintained at 10 DEG C, breaking cellular wall Pollen Pini sterilizing bag completely is packed, standby；
Weighing Radix Rhodiolae, be 0.1MPa at pressure, when temperature 85 DEG C, by water reflux, extract, 3 times, each amount of water is 8 times of Radix Rhodiolae quality, extracts 3 hours, extracting solution 90 mesh screen every time, must extract filtrate, the extraction filtrate of three gained of merging；Extract filtrate concentrate drying, obtain dry powder, pulverize, cross 80 mesh sieves, obtain Radix Rhodiolae extract；
Weighing Radix Ginseng, be 0.1MPa at pressure, when temperature 75 DEG C, by water reflux, extract, 3 times, each amount of water is 8 times of Ginseng Quality, extracts 3 hours every time, and extracting solution crosses 90 mesh screen, must extract filtrate, the extraction filtrate of three gained of merging；Extract filtrate concentrate drying, obtain dry powder, pulverize, cross 100 mesh sieves, obtain Radix Ginseng extract；
Weighing Radix Ophiopogonis, be 0.1MPa at pressure, when temperature 75 DEG C, by water reflux, extract, 2 times, each amount of water is Radix Ophiopogonis 8 times of quality, extracts 3 hours every time, and extracting solution 90 order filters, and must extract filtrate, the extraction filtrate of merging secondary gained；Extract filtrate concentrate drying, obtain dry powder, pulverize, cross 80 mesh sieves, obtain Radix Ophiopogonis extract；
Weigh breaking cellular wall Pollen Pini 290g, Radix Ginseng extract 80g, Radix Rhodiolae extract 170g, Radix Ophiopogonis extract 420g mixes, and crosses 80 mesh sieves, adds microcrystalline Cellulose 300g and forms pharmaceutical formulation, mixing, spraying into 50% Sorbitol 280g is binding agent soft material, granulates with 24 mesh sieves, dried granulate, mixing, tabletting, tablet weight 0.5g, coating, to obtain final product, and composition tablet product is containing Radix Ginseng total saponins >=550mg/100g.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310573472.1A CN103599354B (en) | 2013-11-13 | 2013-11-13 | A kind of pine pollen composition and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310573472.1A CN103599354B (en) | 2013-11-13 | 2013-11-13 | A kind of pine pollen composition and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103599354A CN103599354A (en) | 2014-02-26 |
| CN103599354B true CN103599354B (en) | 2016-07-06 |
Family
ID=50117660
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310573472.1A Active CN103599354B (en) | 2013-11-13 | 2013-11-13 | A kind of pine pollen composition and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103599354B (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105726612A (en) * | 2016-04-08 | 2016-07-06 | 洪海波 | Drug containing herba rhodiolae and phosphatidylserine and preparation method of drug |
| CN106490557A (en) * | 2016-10-19 | 2017-03-15 | 辽宁海吉诺健康品有限公司 | A kind of pine pollen functional food and preparation method thereof |
| CN114515312A (en) * | 2022-03-21 | 2022-05-20 | 烟台新时代健康产业有限公司 | Composition with anti-fatigue effect and preparation method and application thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1365622A (en) * | 2001-06-08 | 2002-08-28 | 烟台新时代天然营养品有限责任公司 | Low-temp airflow pulverization method for pine pollen |
| CN1454527A (en) * | 2003-03-12 | 2003-11-12 | 长春英联医疗保健品有限公司 | Anti-fatigue oral liquor |
| CN201479853U (en) * | 2009-06-24 | 2010-05-26 | 烟台新时代健康产业有限公司 | Pine pollen air flow breaking device with functions of automatic desanding and adjusting wall-breaking rate |
-
2013
- 2013-11-13 CN CN201310573472.1A patent/CN103599354B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1365622A (en) * | 2001-06-08 | 2002-08-28 | 烟台新时代天然营养品有限责任公司 | Low-temp airflow pulverization method for pine pollen |
| CN1454527A (en) * | 2003-03-12 | 2003-11-12 | 长春英联医疗保健品有限公司 | Anti-fatigue oral liquor |
| CN201479853U (en) * | 2009-06-24 | 2010-05-26 | 烟台新时代健康产业有限公司 | Pine pollen air flow breaking device with functions of automatic desanding and adjusting wall-breaking rate |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103599354A (en) | 2014-02-26 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| EP1924273A2 (en) | Plant-based medicament for the treatment of liver disease | |
| CN101357136B (en) | Composition of traditional Chinese medicine effective constituent for preventing and treating diseased associated with cerebral ischemia injury | |
| CN102362881A (en) | Method for preparing ginkgo and American ginseng preparation | |
| CN104784505B (en) | A kind of Dendrobium officinale health product of auxiliary hyperglycemic and preparation method thereof | |
| CN104055871A (en) | Traditional Chinese medicinal composition for improving sleeping quality and application of traditional Chinese medicinal composition | |
| CN102120015A (en) | Traditional Chinese medicine for soothing liver and dispersing depressed vital energy and soothing nerves and sedating mind, and preparation method and quality standard thereof | |
| CN103599354B (en) | A kind of pine pollen composition and application thereof | |
| CN105106816B (en) | Protect the traditional Chinese medicine health care product preparation and preparation method thereof of chemical damage | |
| CN104288245A (en) | Pharmaceutical composition having functions of defying age and enhancing physique, preparation method and detection method thereof | |
| CN103520318B (en) | Traditional Chinese medicine composition of enhancing immune function and preparation method of composition | |
| CN103432421B (en) | Medicine composition for improving immunity | |
| CN103816278B (en) | Composition for reducing blood sugar and application thereof | |
| CN1471845A (en) | Healthy food with glossy and rhizoma as main material and preparing method thereof | |
| CN103735621B (en) | A kind of Chinese medicine composition with blood fat reducing and enhancing immunity effect | |
| CN102552806A (en) | Fat-burning dredging particles and preparation method thereof | |
| CN102357151B (en) | Chinese medicinal composition for delaying senility and preparation process thereof | |
| Semalty et al. | Herbal drugs in chronic fatigue syndrome: an overview | |
| CN111480846A (en) | Health-care product for assisting in reducing blood fat and improving immunity | |
| CN110420258A (en) | A kind of cherokee rose root extract and its preparation method and application with establishing-Yang | |
| CN108379367A (en) | Pure Chinese medicine drug of drug rehabilitation and preparation method thereof | |
| CN110478450B (en) | Pharmaceutical composition for treating Alzheimer disease and application thereof | |
| CN103285110B (en) | Traditional Chinese medicine for treating hyperlipidemia and preparation method thereof | |
| CN107997170A (en) | A kind of composition with hypolipemic function and preparation method thereof | |
| CN106491872A (en) | A kind of health food with auxiliary blood fat reducing and preparation method thereof | |
| CN108066447A (en) | A kind of pharmaceutical composition for preventing depression and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |