CN103598225B - Biological bactericide for controlling paddy rice diseases - Google Patents
Biological bactericide for controlling paddy rice diseases Download PDFInfo
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
A disclosed biological bactericide for controlling paddy rice diseases employs cinnamon essential oil as the active component. Specifically, the biological bactericide is composed of cinnamon essential oil, a surfactant, a co-emulsifier and water, and per Kg of the biological bactericide contains 0.75-75000 mg of cinnamon essential oil, 1.75-175000 mg of the surfactant, 0.75-75000 mg of the co-emulsifier and the balance water. The biological bactericide is mainly used for controlling xanthomonas oryzae pv.oryzae.
Description
Technical field
The invention belongs to rice disease Prevention Technique field, relate in particular to the biological bactericide of a kind of rice disease in field of biological control.
Background technology
China's paddy gross yield was in steady growth situation in recent years, and China is as the big country of rice yield first, particularly important to the disease control of paddy rice.Rice disease has a lot, and wherein bacterial leaf spot disease may occur in each period of paddy growth.First bacterial blight of rice late nineteenth century finds in Japan, has become the important disease in Rice In Asian Cultivated Rice district at present.China East China, Central China, South China rice region occur serious, and also there is distribution in northwest, southwest, North, Northeast China part rice district, and Tibet is also found.
Can only prevent and treat this disease by applying pesticides at present, but remains of pesticide can cause to human body, causes the harm such as cancer, deformity, sudden change acute and chronic poisoning.In recent years, multiple natural plant extracts is used to the study on prevention of carrying out diseases of garden stuff.Plants essential oil is the oily liquids with aromatic odor, normally obtains from the flower of plant, bud, seed, leaf, branch, bark, fruit and root, and is considered to a class GRAS (generally recognized as safe) material.Have multiple suppression mechanism to pathogen, therefore pathogen not easily produces resistance to plants essential oil.But the solvability of plants essential oil in water is extremely low, water-soluble hardly, deposits also unstable, oxidizable in atmosphere under normal temperature, can volatilize with steam.Therefore, improve solvability and the stability of plants essential oil, make its more effective performance bacteriostasis significant.
Summary of the invention
The technical problem to be solved in the present invention is to provide the biological bactericide that one prevents and treats rice disease (particularly bacterial leaf-blight).
In order to solve the problems of the technologies described above, the invention provides a kind of biological bactericide preventing and treating rice disease, the active component of this biological bactericide is cinnamon essential oil.
As the improvement preventing and treating the biological bactericide of rice disease of the present invention: this biological bactericide is made up of cinnamon essential oil, surfactant, assistant for emulsifying agent and water;
In every kg biological bactericide, the assistant for emulsifying agent of the cinnamon essential oil containing 0.75 ~ 75000mg, the surfactant of 1.75-175000mg, 0.75 ~ 75000mg; Surplus is water (being such as distilled water).
As the further improvement preventing and treating the biological bactericide of rice disease of the present invention: in every kg biological bactericide, the assistant for emulsifying agent of the cinnamon essential oil containing 7.5 ~ 750mg, the surfactant of 17.5 ~ 3500mg, 7.5 ~ 750mg.Be more preferred from: in every kg biological bactericide, the assistant for emulsifying agent of the cinnamon essential oil containing 15 ~ 750mg, the surfactant of 70 ~ 3500mg, 15 ~ 750mg.
As the further improvement preventing and treating the biological bactericide of rice disease of the present invention: the mass ratio of cinnamon essential oil and assistant for emulsifying agent is 1:1.
As the further improvement preventing and treating the biological bactericide of rice disease of the present invention: surfactant is Tea Saponin, assistant for emulsifying agent is propionic acid.
As the further improvement preventing and treating the biological bactericide of rice disease of the present invention: surfactant (Tea Saponin) is 4 ~ 5:1 with the mass ratio of cinnamon essential oil.
As the further improvement preventing and treating the biological bactericide of rice disease of the present invention: in every kg biological bactericide, the propionic acid of the cinnamon essential oil containing 0.75g, the Tea Saponin of 3.5g and 0.75g; Surplus is water.
As the further improvement preventing and treating the biological bactericide of rice disease of the present invention: in every kg biological bactericide, the propionic acid of the cinnamon essential oil containing 75mg, the Tea Saponin of 350mg and 75mg; Surplus is water.
As the further improvement preventing and treating the biological bactericide of rice disease of the present invention: rice disease is bacterial leaf-blight fungus diseases.
Microemulsion is a kind of oil, surfactant, cosurfactant and spontaneous isotropism, appearance transparent or translucent, thermodynamically stable dispersion formed of water by certain proportioning, and centrifugal or long-term placement is not easily separated.And research shows, application micro-emulsion technology can improve the solvability of essential oil, strengthens its inhibitory action to pathogen.
The experiment proved that, the biological bactericide preventing and treating rice disease of the present invention can suppress Xanthomonas oryzae effectively, and along with the increase of working concentration, effect strengthens.This essential oil, can safely for the biological control of rice disease to people and animals' no pathogenicity.
In invention process, inventor for raw material, becomes a kind of biological bactericide cinnamon essential oil microemulsion of preventing and treating rice disease through research preparation with cinnamon essential oil, Tea Saponin etc.; This cinnamon essential oil microemulsion is specially adapted to suppress Xanthomonas oryzae.
In invention process, inventor has selected ternary phase diagrams as described in Figure 1, specific as follows:
Three summits on equilateral triangle represent the aqueous phase of 100% content, oil phase and surfactant phase respectively, three limits represent the binary system of aqueous phase-oil phase, oil phase-surfactant phase, surfactant phase-aqueous phase respectively, the relative amount of two components is determined by this distance to the end points in sideline, as A point is depicted as: oil phase 30%, surfactant phase 70%.The system that on sideline, the proportioning of two components that any point and summit line represent on base remains unchanged, as the AB in figure, along from A to B(along with the dropping of aqueous phase), the oil phase in system is 3:7 with the ratio of surfactant phase all the time.In triangle, any point represents ternary system, always forms by determining with the line on an Atria summit and the intersection point of opposite side from this point, consists of oil phase as figure C point: aqueous phase: surfactant phase=7:2:3.(can oil phase be obtained by line BC and the intersection point of limit Oilphase-Surfactant phase: surfactant phase=7:3, can aqueous phase be obtained by line C-Oil phase and the intersection point of limit Aqueousphase-Surfactant phase: surfactant phase=2:3).
Remarks illustrate: above-mentioned oil phase is made up of according to the mass ratio forming 1:1 assistant for emulsifying agent and cinnamon essential oil.
During the actual use of the biological bactericide preventing and treating rice disease of the present invention; Be sprayed in rice field by described biological bactericide, the consumption of every mu is active ingredient---cinnamon essential oil is 0.015-0.75g; Generally sprayed once every 5 days.
The biological bactericide preventing and treating rice disease of gained of the present invention is microemulsion, and it is a kind of solution of clear, and long-time placement can not layering, can show that cinnamon essential oil is dissolved.And cinnamon essential oil is directly dripped to stir in water after what formed is emulsion, can layering after placement, above oil reservoir floats on, so solvability is low and high volatility.The present invention with the addition of surfactant in cinnamon essential oil and then assistant for emulsifying agent mixes with water, and the microemulsion formed can overcome cinnamon essential oil mixes separately formed emulsion above-mentioned defect with water.
Following study on the stability is carried out to the biological bactericide preventing and treating rice disease of gained of the present invention:
(1) dewatering ability:
Adopt constant temperature test method to investigate, get the O/W cinnamon essential oil microemulsion (that is, the biological bactericide preventing and treating rice disease of gained of the present invention) prepared appropriate in 1.5mL centrifuge tube, with the centrifugal 30min of 6000g in supercentrifuge, observe whether phase transformation.Result: do not have phase transformation.
(2) chemical stability:
Quantitatively take the O/W cinnamon essential oil microemulsion for preparing in test tube, slowly drip the NaCl solution of 4mol/L, and concussion evenly, until microemulsion becomes muddy, record adds the quality of NaCl solution.Result: can infinitely add, also can not become muddy.
(3) temperature stability:
Whether packing also seals the O/W cinnamon essential oil micro emulsion prepared, and is placed on 10min in 75 DEG C of constant temperature water baths, observe and undergo phase transition.Result: can not undergo phase transition.
Accompanying drawing explanation
Below in conjunction with accompanying drawing, the specific embodiment of the present invention is described in further detail.
Fig. 1 is typical ternary phase diagrams.
Fig. 2 is the effect contrast figure that the external flat board of variable concentrations microemulsion to leaf spot bacteria suppresses;
Whether the otherness of the effect produced between each concentration that what a b c of small letter represented is is remarkable, if what pillar marked is all same letter, illustrates that the fungistatic effect under these two kinds of concentration is the same, do not have otherness;
Fig. 3 is the effect contrast figure that the external shaking flask of variable concentrations microemulsion to leaf spot bacteria suppresses;
Fig. 4 is the control efficiency figure of variable concentrations microemulsion to bacterial leaf-blight evil.
Fig. 5 is for the microemulsion described in comparative example is to the control efficiency figure of bacterial leaf-blight evil.
Embodiment
The preparation of embodiment 1, cinnamon essential oil microemulsion (preventing and treating the biological bactericide of rice disease):
One, stoste is prepared:
1), the preparation of Tea Saponin solution: be dissolved in by 100g Tea Saponin in 100mL distilled water, preparation mass fraction is the Tea Saponin solution of 50%.
2), by Tea Saponin solution, cinnamon essential oil, propionic acid and distillation aqueous phase mix, obtain stoste; Cinnamon essential oil, the Tea Saponin (SP) of 350g, the propionic acid of 75g containing 75g in described every kg stoste; Surplus is water (distilled water).
Above-mentioned stoste can deposit in 25 DEG C of incubators.In above-mentioned stoste corresponding diagram 2 I.
Two, the biological bactericide of following various water prevention rice disease is mixed with:
1) microemulsion (10 of 10 times is diluted
-1c): draw 0.5g stoste in the distilled water of 4.5g, shake up; The biological bactericide II of rice disease must be prevented and treated.
In the above-mentioned biological bactericide II of every kg, the Tea Saponin (SP) of the cinnamon essential oil containing 7.5g, 35g, the propionic acid of 7.5g; Surplus is water (distilled water).
2) microemulsion (10 of 100 times, is diluted
-2c): draw 0.05g stoste in the distilled water of 4.95g, shake up.The biological bactericide III of rice disease must be prevented and treated.
In the above-mentioned biological bactericide III of every kg, the Tea Saponin (SP) of the cinnamon essential oil containing 0.75g, 3.5g, the propionic acid of 0.75g; Surplus is water (distilled water).
3) microemulsion (10 of 1000 times, is diluted
-3c): draw 0.005g stoste in the distilled water of 4.995g, shake up.The biological bactericide IV of rice disease must be prevented and treated.
In the above-mentioned biological bactericide IV of every kg, the Tea Saponin (SP) of the cinnamon essential oil containing 75mg, 350mg, the propionic acid of 75mg; Surplus is water (distilled water).
4) microemulsion (5 × 10 of 5000 times, is diluted
-3c): draw 0.001g stoste in the distilled water of 4.999g, shake up.The biological bactericide V of rice disease must be prevented and treated.
In the above-mentioned biological bactericide V of every kg, the Tea Saponin (SP) of the cinnamon essential oil containing 15mg, 70mg, the propionic acid of 15mg; Surplus is water (distilled water)..
5) microemulsion (10 of 10000 times, is diluted
-4c): draw 0.0005g stoste in the distilled water of 4.9995g, shake up.The biological bactericide VI of rice disease must be prevented and treated.
In the above-mentioned biological bactericide VI of every kg, the Tea Saponin (SP) of the cinnamon essential oil containing 7.5mg, 35mg, the propionic acid of 7.5mg; Surplus is water (distilled water).
Remarks illustrate: in above-mentioned biological bactericide II ~ biological bactericide VI corresponding diagram 2 ~ Fig. 4 II ~ VI.
The above-mentioned biological bactericide preventing and treating rice disease is tested respectively as follows:
One, the external flat board of cinnamon essential oil microemulsion to leaf spot bacteria suppresses
(1) leaf spot bacteria is activated.
(2) the PDA medium of thawing is poured in culture dish, prepare 24 flat boards.4 one group is divided into 6 groups.
(3) picking leaf spot bacteria mixes in sterile water, surveys its OD value and is about 0.5.
(4) draw 100 μ L step (3) gained bacterium liquid in the flat board prepared, with spreading rod, bacterium liquid is smoothened spreadable.
(5) plate center (Oxford cup should not insert medium too deeply also should not be too shallow) is placed in tweezers gripping Oxford cup.
(6) often organize each absorption 200 μ L cinnamon essential oil microemulsion in the cup of Oxford, be put in 3 flat boards respectively, as process; Draw 200 μ L sterile waters in the cup of Oxford, be placed in 1 other flat board, in contrast.
(7) cultivate 2 days in 28 DEG C of incubators.
(8) antibacterial circle diameter is measured.
Acquired results as shown in Figure 2.
According to Fig. 2, we learn: under the effect of microemulsion I (i.e. stoste) and II (i.e. biological bactericide II), and inhibition zone appears in flat board, and its diameter is about 90mm and 32mm respectively; Under other concentration microemulsion effect, there is not inhibition zone, consistent with blank; Illustrate that micro emulsion stoste I and II significantly can suppress the growth of leaf spot bacteria, and other concentration microemulsion do not have inhibitory action to the growth of leaf spot bacteria in flat board.
Two, the external shaking flask of cinnamon essential oil microemulsion to leaf spot bacteria suppresses
(1) bacterial leaf spot pathogen is activated;
(2) add potato dextrose broth in test tube, in high-pressure sterilizing pot, sterilizing is stand-by.
(3) picking leaf spot bacteria mixes in sterile water, surveys its OD value and is about 0.5.
(4) on superclean bench, add in all test tubes by bacterial leaf spot bacterium liquid, the test tube wherein only adding sterile water as a control group, adds the test tube of microemulsion as experimental group.
Be specially: each test tube adds bacterial leaf spot bacterium liquid 0.1mL; Correspondence adds 0.5mL biological bactericide II, biological bactericide III, biological bactericide IV, biological bactericide V, biological bactericide VI respectively again; Last each test tube adds culture fluid (step (2) gained) and supplies 5mL.
(5) cultivate on 28 DEG C of shaking tables.
(6) OD value is surveyed.
Acquired results as shown in Figure 3.
According to Fig. 3, we learn: control group bacterial leaf spot bacterium liquid OD value is maximum, and under microemulsion II-IV acts on, bacterium liquid OD value is 0, and under microemulsion VI acts on, OD value is only second to contrast; Illustrate that microemulsion II-V completely or almost can suppress the growth of leaf spot bacteria completely, microemulsion VI can partly suppress it to grow.
Three, cinnamon essential oil microemulsion is to the vivo control of bacterial leaf-blight evil
(1) bacterium solution preparation: picking leaf spot bacteria mixes in sterile water, surveys its OD value and is about 0.5.
(2) paddy rice is divided into 6 groups, often organizes 10 strains, be respectively 1 control group and 5 experimental group.Select the newborn blade launched for subsequent use.
(3) scissors is immersed the bacterium liquid prepared, on the upside of the blade of select, cut an angle, before cutting, will again soak bacterium liquid at every turn.
In this, as control group.
(4) one group of blade that the side of getting step (3) gained is cut, the cinnamon essential oil microemulsion of 20 μ L variable concentrations is drawn (namely with liquid-transfering gun, biological bactericide II, biological bactericide III, biological bactericide IV, biological bactericide V, biological bactericide VI) be applied to angle, as experimental group.
(5) situation occurred of rice leaf bacterial leaf spot disease is observed the next day.
Acquired results as shown in Figure 4.
According to Fig. 4, we learn: employing microemulsion III ~ V does the generation that biological bactericide all significantly can suppress bacterial blight of rice evil, and wherein the inhibition of microemulsion III is best.And for microemulsion II, its rice leaf lesion diameter than the also large reason of contrast is on the contrary, essential oil concentration is too high has facilitation to rice disease.
Comparative example 1-1, a kind of biological bactericide preventing and treating rice disease, only make Tea Saponin into Tween-80, all the other are equal to biological bactericide III.
Comparative example 1-2, a kind of biological bactericide preventing and treating rice disease, only make Tea Saponin into Tween-20, all the other are equal to biological bactericide III.
Comparative example 2-1, a kind of biological bactericide preventing and treating rice disease, only make propionic acid into absolute ethyl alcohol, all the other are equal to biological bactericide III.
Comparative example 2-2, a kind of biological bactericide preventing and treating rice disease, only make propionic acid into glycerine, all the other are equal to biological bactericide III.
Comparative example 3-1, a kind of biological bactericide preventing and treating rice disease, only make the content of propionic acid into 0.5g by 0.75g, all the other are equal to biological bactericide III.
Comparative example 3-2, a kind of biological bactericide preventing and treating rice disease, only make the content of propionic acid into 1g by 0.75g, all the other are equal to biological bactericide III.
The use of comparative example 4, cancellation Tea Saponin,
That is, in the above-mentioned biological bactericide of every kg, the cinnamon essential oil containing 0.75g, the propionic acid of 0.75g; Surplus is water (distilled water)..
The use of comparative example 5, cancellation propionic acid,
In the biological bactericide of every kg, the Tea Saponin (SP) of the cinnamon essential oil containing 0.75g, 3.5g; Surplus is water (distilled water)..
The use of comparative example 6, simultaneously cancellation Tea Saponin and propionic acid,
That is, in the biological bactericide of every kg, the cinnamon essential oil containing 0.75g; Surplus is water (distilled water)..
Above-mentioned all comparative examples tested according to above-mentioned " cinnamon essential oil microemulsion is to the vivo control effect of bacterial leaf-blight evil ", acquired results as shown in Figure 5.
Finally, it is also to be noted that what enumerate above is only several specific embodiments of the present invention.Obviously, the invention is not restricted to above embodiment, many distortion can also be had.All distortion that those of ordinary skill in the art can directly derive from content disclosed by the invention or associate, all should think protection scope of the present invention.
Claims (2)
1. the application in rice disease prevented and treated by biological bactericide, and the active component of this biological bactericide is cinnamon essential oil; In described every kg biological bactericide, the propionic acid of the cinnamon essential oil containing 0.75g, the Tea Saponin of 3.5g and 0.75g; Surplus is water.
2. the application in rice disease prevented and treated by biological bactericide according to claim 1, it is characterized in that: described rice disease is bacterial leaf-blight fungus diseases.
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| BR112017000039A2 (en) * | 2014-07-02 | 2018-07-17 | Ralco Nutrition, Inc. | agricultural compositions and applications using essential oils |
| CN110833076A (en) * | 2019-11-22 | 2020-02-25 | 贵州省油料研究所 | Rosemary essential oil microemulsion for preventing and treating plant diseases and insect pests and preparation method thereof |
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| CN103598225A (en) | 2014-02-26 |
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Effective date of registration: 20191225 Address after: 314416 No.318, Nanjie Road, Yuanhua Town, Haining City, Jiaxing City, Zhejiang Province Patentee after: Haining Yuanhua Town Industrial Investment Co.,Ltd. Address before: 321004 Zhejiang Province, Jinhua city Wucheng District Yingbin Road No. 688 Patentee before: Zhejiang Normal University |
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Denomination of invention: Biological fungicides for preventing and controlling rice diseases Effective date of registration: 20231229 Granted publication date: 20150715 Pledgee: Zhejiang Haining Rural Commercial Bank Co.,Ltd. Yuanhua sub branch Pledgor: Haining Yuanhua Town Industrial Investment Co.,Ltd. Registration number: Y2023980074556 |