CN103598078B - Summer floating-seedling method of barley tissue culture seedlings - Google Patents
Summer floating-seedling method of barley tissue culture seedlings Download PDFInfo
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- CN103598078B CN103598078B CN201310648253.5A CN201310648253A CN103598078B CN 103598078 B CN103598078 B CN 103598078B CN 201310648253 A CN201310648253 A CN 201310648253A CN 103598078 B CN103598078 B CN 103598078B
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Abstract
A summer floating-seedling method of barley tissue culture seedlings is characterized in that a seedling shed, a floating pond and a floating plate are used, as for floating-seedling of the tissue culture seedlings, firstly floating-seedling of the tissue culture seedlings is carried out through a floating-seedling technique, then cultivation is carried out through nutrition bags so that the problem of the survival rate can be solved, the survival rate can reach 90%-98%, pest and plant diseases and insect pests are effectively controlled, the seedlings are strong, the root systems are flourishing and quality of seeds is effectively improved. According to the summer floating-seedling method, the problem of the survival rate of the barley tissue culture seedlings is solved, the yields and the quality of barley are improved, the seed multiplication cycle and the breeding cycle of the barley are shortened, and a critical function of improving a new variety of the barley is achieved.
Description
Technical field
The present invention relates to agriculture plantlet in vitro floating seedlings technology, especially a kind of barley plantlet in vitro floating seedlings culturing method in summer.
Background technology
Conventional barley plantlet in vitro is that directly plantation is in booth, and the deficiencies in the prior art part is: there is survival rate low, and survival rate can only reach 40%-50%, and damage by disease and insect is many, not easily convenient management.The advantages such as need the new method of inventing a kind of barley plantlet in vitro floating seedlings in summer, have cost rate in low strong sprout high, survival rate brings up to 90%-98% from original 40%-50%, and damage by disease and insect is few, easy and simple to handle, convenient management.
Conventional barley plantlet in vitro is that directly plantation is in booth, and there is survival rate low, survival rate can only reach 40%-50%, and damage by disease and insect is many, not easily convenient management.After improving, first use floating seedlings technology after plantlet in vitro floating seedlings, then solve survival rate with nutritious bag cultivation, survival rate to 90%-98%, and effectively controls damage by disease and insect, Miao Zhuan, well developed root system.Seed quality is effectively improved.
The present invention solves barley plantlet in vitro survival rate, improves barley yield and quality, shortens the cycle of barley breeding, breeding, gets critical effect to New Barley Variety improvement.
Summary of the invention
A kind of barley plantlet in vitro floating seedlings culturing method in summer, is characterized in that: adopt seedling growth greenhouse, floating pool, floating disc,
Plantlet in vitro floating seedlings, described barley plantlet in vitro floating seedlings culturing method in summer comprises the following steps:
(1) booth is built: the form-factor of booth, and canopy is high is not less than 250cm, width 450cm, possesses the ventilation door and window and fly net that can open arbitrarily, and canopy film is without dripping long-lived film;
(2) construction of floating pool: the Chi Geng hollow brick of floating pool or plank or sun-dried mud brick are made, or with concrete casting, require that ridge face width degree is no less than 40cm, face, the ridge and pond periphery keep straight and guarantee that floating disc puts into rear tight;
(3), after Chi Geng carries out, at the bottom of levelling pond, level up at the bottom of pond with sand or straw or fine earth, prevent sand, stone, grass, stalk from scratching pond film;
(4) place sterilization: floating pool is built up rear use 0.1% liquor potassic permanganate or 40% and educated precious 200 times of liquid to around place, floating pool, and bow member carries out spraying disinfection once;
(5) plastic film mulch: the pond counterdie film of thickness more than 8;
(6) add water: in floating pool, add running water, the river water of flowing or reservoir water or well water, the degree of depth of water, with 8-10cm, when discovery water becomes the anomaly such as muddy, smelly in seedling raising process, should change water in time;
(7) barley plantlet in vitro dedicated substrate formula
Peat composed of rotten mosses 40%-45%, brown coal 10%-8%, vermiculite 8%-5%, humus soil 15%-20%, perlite 26.5%-21%, micro-0.5%-1%;
(8) specification of floating disc: the polystyrene plastics foam lattice dish adopting 162 holes; Specification is 66.6cm × 33.2cm × 6.5cm, and density is more than 20 orders, weight 180 grams;
(9) sterilization of floating disc: floating disc disinfecting time is for transplanting seedlings first 15 days, concrete grammar is: put into by floating disc and the pond that 0.1% liquor potassic permanganate or 40% educates precious 200 times of liquid is housed soaks 10 minutes, taking-up concentrates on clean place and dries, can use, above disinfection must complete for 10 days before transplanting seedlings;
(10) sabot
Matrix water balance: before sabot, matrix water content should be adjusted to 30%-40%, namely water supply in media content reach that hand pinches can be agglomerating, the nature that lands scatters and is advisable or puts into floating pool after medium in tray, and within 1-3 minute, whole holes absorb water;
Sabot: medium in tray is layered on dish by matrix, with 3 times, have gentle hands bat dish side or lift and coil overhead 20cm free-falling 2 times after being paved with whole hole, repaving matrix, scrapes off matrix unnecessary in card with hand;
(11) management during transplanting seedlings
Plantlet in vitro is transplanted to drift dish, strictly controls greenhouse temperature, less than 20 DEG C, and select overcast and rainy best results of transplanting seedlings, large ceiling must draw the sunshade net of shading rate 90%;
Plantlet in vitro coils to move 20-35 days nutritious bag cycles from moving drift;
Drift seedling was by 10 days, and liquid manure 8 grams executed by each drift dish, and N P and K is the composite fertilizer special of 15:15:15, and fertilizer is evenly poured in pond after being placed on and being dissolved in water in a keg, floated necessary strict guarantee ventilation, ventilative, sunshade between seedling stage;
Apply fertilizer after 1 month, administration period supplements appropriate Chi Shui, observes seedling change, and as there is the process in time such as white powder, rust, the growing way situation according to seedling determines transplanting time, is generally no more than 2 months.
Altogether test drift seedling 6828 strain in 2013, kind 54 kinds, survives several 6623, survival rate 97%.
A kind of barley plantlet in vitro of the present invention floating seedlings culturing method in summer, substantial feature and marked improvement are: first use floating seedlings technology after plantlet in vitro floating seedlings, then solve survival rate with nutritious bag cultivation, survival rate is to 90%-98%, and effectively control damage by disease and insect, Miao Zhuan, well developed root system.Seed quality is effectively improved.
The present invention solves barley plantlet in vitro survival rate, improves barley yield and quality, shortens the cycle of barley breeding, breeding, gets critical effect to New Barley Variety improvement.
Embodiment
Embodiment 1
The advantages such as implement a kind of barley plantlet in vitro floating seedlings culturing method in summer, have cost rate in low strong sprout high, survival rate brings up to 90%-98% from original 40%-50%, and damage by disease and insect is few, easy and simple to handle, convenient management.Adopt seedling growth greenhouse, floating pool, floating disc, plantlet in vitro floating seedlings, described barley plantlet in vitro floating seedlings culturing method in summer comprises the following steps:
One, the preparation of floating disc
(1) specification: a kind of polystyrene plastics foam lattice dish of employing 162 hole; Specification is 66.6cm × 33.2cm × 6.5cm, and density is more than 20 orders, weight 180 grams.
(2) sterilize: the floating disc not disease carrying germ that first time uses, need not sterilize before using.But new building is in keeping and use procedure, note may not carrying disease germs with soil, muck etc., with malicious object contact.Used floating disc may bring germ, must sterilize, and disinfecting time is for transplanting seedlings first 15 days.Concrete grammar is: put into by floating disc and the pond that 0.1% liquor potassic permanganate or 40% educates precious 200 times of liquid is housed soaks 10 minutes, takes out to concentrate on clean place and dry, and can use.More than work and must complete for 10 days before transplanting seedlings.
Two, the selection in nursery place
Nursery site requirements is leeward on the sunny side, pollution-free, and water source is convenient, and draining is smooth and easy, traffic convenience, easily smooth.
Three, the construction of seedling growth greenhouse and floating pool
1, booth is built: the form-factor of booth, frame material can according to actual conditions sets itself.Requirement frame is firm, wind resistance, rain, snow.Canopy is high is not less than 250cm, width 450cm, possesses the ventilation door and window and fly net that can open arbitrarily, guarantees that temperature of shed regulation and control are sensitive.Canopy film, without dripping a long-lived film, prevents the globule to drip the matrix washed away in lattice dish from canopy film, causes hole.
2, the construction of floating pool: the specification according to canopy builds pond with peak use rate, the specification of floating pool is grasped flexibly according to the specification of canopy.Chi Geng can make with hollow brick, plank or sun-dried mud brick, also can use concrete casting, requires that ridge face width degree is no less than 40cm, and face, the ridge and pond periphery keep straight and guarantee that floating disc puts into rear basic tight, as more coarse at the bottom of pond, can spread a small amount of fine earth or fine sand water detection is put down.
3, after smooth location, strict with above specification construction floating pool, guarantee that floating disc puts into tight after floating pool, if floating pool is built lack of standardization, floating disc just has too much space after putting into, the water surface of gap exposes will soon grow the algae such as moss in the sun, and algal grown can consume oxygen in flotation fluid and nutrient, affects seedling and grows.
4, Chi Geng adopts the width ridge, and available hollow brick, common brick, sun-dried mud brick are made.With the Geng Zhai ridge, the pond 30cm that hollow brick or common brick code become, wide ridge 50cm.
5, after Chi Geng carries out, at the bottom of levelling pond, level up at the bottom of pond with sand, straw, fine earth etc., prevent sand, stone, grass, stalk from scratching pond film.
6, place sterilization: floating pool is built up rear use 0.1% liquor potassic permanganate or 40% and educated precious 200 times of liquid to around place, and floating pool, bow member carries out spraying disinfection once.
7, plastic film mulch: the film of pond counterdie thickness more than 8 is advisable, and will prevent sandstone during plastic film mulch, and stalk etc. scratch pond film, and plastic film mulch is smooth.
8, add water: in floating pool, add running water, the river water of flowing, reservoir water, the water that well water etc. are clean, prohibits the use that do not flow in pool, puddle, ditch etc., unclean water, and the water in these places carries the germs of a disease, and can infect seedling.The degree of depth of water is advisable with about 8-10cm, as found the anomalies such as water becomes muddiness, smelly in seedling raising process, should change water in time, otherwise will affect seedling growth.
Four, barley plantlet in vitro dedicated substrate formula
A, the peat composed of rotten mosses 40%, B, brown coal 9%, C, vermiculite 5%, D, humus soil 20%, E, perlite 25%, F, trace element 1%.
Five, sabot
1, matrix water balance: before sabot, matrix water content should be suitable for, overdrying sabot matrix is easily missed and is caused hole and hole hollow not to absorb water to form dry cave, cross wet sabot easily to cause hole hollow not absorb water to form dry cave, affect planting percent, therefore should regulate matrix water content before sabot.Specifically: before sabot, matrix water content should be adjusted to about 30%-40%, namely water supply in media content reach that hand pinches can be agglomerating, the nature that lands scatters and is advisable or puts into floating pool after medium in tray, within 1-3 minute, ensures that whole hole absorbs water.
2, sabot: medium in tray degree of tightness should be moderate, concrete grammar is layered on dish by matrix, with 3 times, have gentle hands bat dish side or lift and coil overhead 20cm free-falling 2 times after being paved with whole hole, repaves matrix, scrape off matrix unnecessary in card with hand, attention can not use finger or waddy etc.Compacting matrix can not do dress can not hole.
Six, management during transplanting seedlings
1, plantlet in vitro is transplanted to drift dish, strictly controls greenhouse temperature, less than 20 DEG C, and select overcast and rainy best results of transplanting seedlings, large ceiling must draw the sunshade net of shading rate 90%, transplants seedlings the last week, and personnel must stand fast at booth.
2, plantlet in vitro coils to move 20-35 days general cycles of nutritious bag from moving drift, sees that seedling growing way situation determines transplanting time.
3, float seedling by about 10 days, liquid manure 8 grams executed by each drift dish, and N P and K is the composite fertilizer special of 15:15:15, fertilizer is placed in a keg to add after little water is dissolved and evenly pours in pond, float necessary strict control between seedling stage, ventilation, ventilative, sunshade, temperature must keep neutral temperature.
4, determine fertilizing amount according to seedling growing way after 1 month, administration period supplements appropriate Chi Shui, observes seedling change, and as there is the process in time such as white powder, rust, the growing way situation according to seedling determines transplanting time, is generally no more than 2 months.
Embodiment 2
The advantages such as implement a kind of barley plantlet in vitro floating seedlings culturing method in summer, have cost rate in low strong sprout high, survival rate brings up to 90%-98% from original 40%-50%, and damage by disease and insect is few, easy and simple to handle, convenient management.Adopt seedling growth greenhouse, floating pool, floating disc, plantlet in vitro floating seedlings, described barley plantlet in vitro floating seedlings culturing method in summer comprises the following steps:
Barley plantlet in vitro dedicated substrate is filled a prescription: A, the peat composed of rotten mosses 40%, B, brown coal 10%, C, vermiculite 8%, D, humus soil 15%, E, perlite 26.5%, F, trace element 0.5%.
All the other are identical with embodiment 1 step, repeat no more.
Claims (1)
1. a barley plantlet in vitro floating seedlings culturing method in summer, is characterized in that: adopt seedling growth greenhouse, floating pool, floating disc, plantlet in vitro floating seedlings, described barley plantlet in vitro floating seedlings culturing method in summer comprises the following steps:
(1) booth is built: the form-factor of booth, and canopy is high is not less than 250cm, width 450cm, possesses openable ventilation door and window and fly net, and canopy film is without dripping long-lived film;
(2) construction of floating pool: the Chi Geng hollow brick of floating pool or plank or sun-dried mud brick are made, or with concrete casting, require that ridge face width degree is no less than 40cm, face, the ridge and pond periphery keep straight and guarantee that floating disc puts into rear tight;
(3), after Chi Geng carries out, at the bottom of levelling pond, level up at the bottom of pond with sand or straw or fine earth, prevent sand, stone, grass, stalk from scratching pond film;
(4) place sterilization: floating pool is built up rear use 0.1% liquor potassic permanganate or 40% and educated precious 200 times of liquid to around place, floating pool, and bow member carries out spraying disinfection once;
(5) plastic film mulch: the pond counterdie film of thickness more than 8;
(6) add water: in floating pool, add the river water of running water or flowing or reservoir water or well water, the degree of depth of water is 8-10cm, and when discovery water becomes muddy in seedling raising process, smelly anomaly, should change water in time;
(7) barley plantlet in vitro dedicated substrate formula
Peat composed of rotten mosses 40%-45%, brown coal 10%-8%, vermiculite 8%-5%, humus soil 15%-20%, perlite 26.5%-21%, micro-0.5%-1%;
(8) specification of floating disc: the polystyrene plastics foam lattice dish adopting 162 holes; Specification is 66.6cm × 33.2cm × 6.5cm, and density is more than 20 orders, weight 180 grams;
(9) sterilization of floating disc: floating disc disinfecting time is for transplanting seedlings first 15 days, concrete grammar is: put into by floating disc and the pond that 0.1% liquor potassic permanganate or 40% educates precious 200 times of liquid is housed soaks 10 minutes, taking-up concentrates on clean place and dries, can use, above disinfection must complete for 10 days before transplanting seedlings;
(10) sabot
Matrix water balance: before sabot, matrix water content should be adjusted to 30%-40%, namely water supply in media content reach that hand pinches can be agglomerating, the nature that lands scatters and is advisable or puts into floating pool after medium in tray, and within 1-3 minute, whole holes absorb water;
Sabot: medium in tray is layered on dish by matrix, with 3 times, have gentle hands bat dish side or lift and coil overhead 20cm free-falling 2 times after being paved with whole hole, repaving matrix, scrapes off matrix unnecessary in card with hand;
(11) management during transplanting seedlings
Plantlet in vitro is transplanted to drift dish, strictly controls greenhouse temperature, remains on less than 20 DEG C, and select overcast and rainy best results of transplanting seedlings, large ceiling must draw the sunshade net of lid shading rate 90%;
Plantlet in vitro coils to move 20-35 days nutritious bag cycles from moving drift;
Drift seedling was by 10 days, and liquid manure 8 grams executed by each drift dish, and N P and K is the composite fertilizer special of 15:15:15, and fertilizer is evenly poured in pond after being placed on and being dissolved in water in a keg, floated necessary strict guarantee ventilation, ventilative, sunshade between seedling stage;
Apply fertilizer after 1 month, administration period supplements appropriate Chi Shui, and observe seedling change, as there is white powder, rust processes in time, and the growing way situation according to seedling determines transplanting time, is generally no more than 2 months.
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| CN104996132B (en) * | 2015-06-30 | 2018-03-27 | 徐宁 | A kind of implantation methods of high altitude localities landscape barley |
| CN105284569A (en) * | 2015-11-20 | 2016-02-03 | 宁书昌 | Gallnut moss stem cutting reproduction and flotation culture method |
| CN106171920B (en) * | 2016-07-18 | 2019-03-12 | 湖南文理学院 | A kind of pool Ecology seedbed construction method |
| CN110771303A (en) * | 2019-10-21 | 2020-02-11 | 哈尔滨市农业科学院 | Moisture-preserving seedling-raising and seedling-strengthening device for promoting seed germination and seedling rooting |
| CN114303911B (en) * | 2020-12-19 | 2023-12-05 | 李福元 | Method for hardening seedlings by out-of-bottle soilless transplanting of sugarcane detoxified tissue culture root seedlings |
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| CN103039225B (en) * | 2012-11-26 | 2014-03-12 | 李奋勇 | Floating exercising seedling and cultivating method of dendrobium officinale tissue culture seedling |
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Effective date of registration: 20160304 Address after: 650100, No. 8, No. 802, building S8, triumph garden, South three ring road, Xishan District, Yunnan, Kunming Patentee after: Kunming Yingwu agricultural science and Technology Co Ltd Address before: 650106 S07-3-502, South Garden, triumph garden, RI Zhong Lu, Kunming, Yunnan Patentee before: Xu Ning |