CN103595864A - Microfluidic chip liquid control system based on wireless mobile intelligent terminal - Google Patents
Microfluidic chip liquid control system based on wireless mobile intelligent terminal Download PDFInfo
- Publication number
- CN103595864A CN103595864A CN201310547782.6A CN201310547782A CN103595864A CN 103595864 A CN103595864 A CN 103595864A CN 201310547782 A CN201310547782 A CN 201310547782A CN 103595864 A CN103595864 A CN 103595864A
- Authority
- CN
- China
- Prior art keywords
- micro
- intelligent terminal
- chip
- mobile intelligent
- wireless mobile
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Abstract
A microfluidic chip liquid control system based on a wireless mobile intelligent terminal comprises a microfluidic chip module, a wireless drive control module and the wireless mobile intelligent terminal with a wifi function. The microfluidic chip module is in two-way communication and connection with the wireless drive control module. The wireless drive control module is in two-way communication and connection with the wireless mobile intelligent terminal with the wifi function. The wireless mobile intelligent terminal is adopted, and movement of fluid in a microfluidic chip is driven/controlled through wireless signals, various types of control over the fluid is achieved, and the microfluidic chip liquid control system has the advantages of being simple in system, low in cost, flexible in control and high in expansibility.
Description
Technical field
The present invention relates to automatic control, relate in particular to a kind of micro-fluidic chip liquid control system based on wireless mobile intelligent terminal.
Background technology
Micro-fluidic chip technology (Microfluidics) is that the basic operation units such as the sample preparation of biological, chemistry, medical analysis process, reaction, separation, detection are integrated on a small chip, automatically complete whole analytic process (Whitesides, G.M.Nature2006,442,368-373).Micro-fluidic chip is the main platform that microflow control technique is realized, and its device characteristic is mainly that the resulting structure (passage, reative cell and some other functional part) of its containing fluid is at least micron order yardstick in a dimension.Due to micron-sized structure, fluid shows therein and produced the property different from macro-scale, therefore develop unique analyzing and testing performance (Blow, N.Nature methods2009,6,683-686).Micro-fluidic chip have liquid flow controlled, consume sample and the feature such as reagent is few, analysis speed is fast, it is analyzed when can carry out up to a hundred samples within even shorter time a few minutes, and preliminary treatment that can canbe used on line sample and analyze overall process, therefore be widely used in the detection (Yager of clinical biomedical sample, P., et al.Nature2006,442,412-418; Doerr, A.Nature methods2009,6,194-194).For example, micro-fluidic drop round pcr can be used for cell DNA order-checking (Tewhey, R., et al.Nature Biotechnology2009,27,1025-1031), micro-fluidic pump valve chamber technology can be used for single cell protein group and detects (Wei, W., et al.PNAS2013,110, E1352-E1360), and microfluidic channel micrometer structure and silicon array can be used for the (Nagrath that catches of rare circulating tumor cell in blood, S., et al.Nature2007,450,1235-1239; Dong, Y., et al.Journal of Molecular Diagnostics2013,15,149-157).
Although micro-fluidic chip has the features such as microminiaturization, controlledization, integrated, automation, but its peripheral support equipment is complicated, based on machinery or electromagnetic pump, valve, drive in the micro-fluidic chip Medical Devices of controlling, mostly adopt controller, or it is combined with computer and by computer, drives the mode of control liquid, the former cannot realize control and the operation of automation, the latter need to, by computer, increase equipment cost, volume and energy consumption (Blow, N.Nature methods2007,4,665-668; Rosen, Y. & Gurman, P. Current Pharmaceutical Biotechnology2010,11,366-375).In recent years, mobile intelligent terminal technology develops rapidly, and smart mobile phone, panel computer are universal in a large number.Have at present the method (Mao that controls the liquid in micro-fluidic chip by mobile intelligent terminal, X. & Huang, T.J.Lab Chip2012,12,1412-1416), as the Electrochemical Detection based on mobile phone (Lillehoj, P., Huang, M.-C. & Ho, C.-M.2013IEEE26th Intemational Conference on53-56), the sweat based on smart mobile phone, saliva pH detect (Oncescu, V., O'Dell, D. & Erickson, D.Lab Chip2013,13,3232-3238).More than detect and all adopt data wire to connect mobile phone and chip, but the use of data wire has limited the use of command range, multi-multipoint control etc., not convenient.Therefore, with the liquid that mobile wireless device (smart mobile phone, panel computer etc.) is controlled in micro-fluidic chip by wireless signal, have important practical significance.Meanwhile, mobile intelligent terminal can also substitute the function of the display screen of controlled microfluidic device, thereby further simplifies the design of microfluidic device and reduce its volume.
The present invention is the requirement to fluid flow and the accurate control of reaction mainly for micro-fluidic chip, the micro electronmechanical mechanisms such as Micropump, micro-valve are combined with micro-fluidic chip, realize the various controls of convection cell, there are two kinds of mode of operations of the manual mode of standalone feature operation and the automatic mode of multiple standalone feature combination operation.
Summary of the invention
Object of the present invention, exactly in order to provide a kind of micro-fluidic chip liquid control system based on wireless mobile intelligent terminal.
In order to achieve the above object, the present invention has adopted following technical scheme: a kind of micro-fluidic chip liquid control system based on wireless mobile intelligent terminal, comprises micro-fluidic chip module, wireless drive control module and have the wireless mobile intelligent terminal of wifi function; Micro-fluidic chip module is connected with wireless drive control module both-way communication, and wireless drive control module is connected with the wireless mobile intelligent terminal both-way communication with wifi function by wireless network.
Described micro-fluidic chip module comprises the micro-fluidic chip that does not contain pump, valve arrangement and the micro-fluidic chip that contains pump, valve arrangement.
Described wireless drive control module comprises wifi chip, Bluetooth chip, infrared chip, single-chip microcomputer and power supply, and wifi chip, Bluetooth chip and infrared chip are connected with single-chip microcomputer two-way communication respectively, and power supply is connected and powers to single-chip microcomputer with monolithic.
The wireless mobile intelligent terminal of the described wifi of having function is smart mobile phone, panel computer or intelligent MP4.
In the wireless mobile intelligent terminal of the described wifi of having function, be provided with fluid and control software, by this fluid control software can control fluid in micro-fluidic chip flow with stop, the currency of fluid and the flow velocity of fluid.
By fluid, control software and can realize a wireless mobile intelligent terminal and control a plurality of micro-fluidic chips, or a plurality of wireless mobile intelligent terminal is shared a micro-fluidic chip.
The present invention compared with prior art, has following advantage and disadvantage:
1) system simplification: not needing, for each micro-fluidic chip and control system configuration computer, does not need even display screen of data wire, has reduced the restriction of hardware to system.
2) cost is low: owing to adopting mobile wireless device (as smart mobile phone, panel computer), user only need to oneself mobile phone or panel computer on downloading application software just can use micro-fluidic chip equipment, therefore a plurality of users can share a micro-fluidic chip equipment, have reduced use cost.
3) control flexibly: use wifi to control, can control a plurality of micro-fluidic chips by a mobile wireless device, be convenient to the collection analysis of medical centre to data, also can share a micro-fluidic chip by a plurality of mobile wireless devices, allowing equipment share becomes possibility.
4) expansibility is strong: because use mobile wireless device to control, can utilize it to make, scan Quick Response Code, storage transmission information, realizes personalized treatment; Can also network by mobile wireless device, realize remote diagnosis.
Accompanying drawing explanation
Fig. 1 is the structured flowchart that the present invention is based on the micro-fluidic chip liquid control system of wireless mobile intelligent terminal;
Fig. 2 is the structured flowchart of the wireless drive control module in the present invention;
Fig. 3 is the structured flowchart of one embodiment of the invention;
Fig. 4 is the structured flowchart that in the present invention, the fluid in wireless mobile intelligent terminal is controlled software.
Embodiment
Referring to Fig. 1, the present invention is based on the micro-fluidic chip liquid control system of wireless mobile intelligent terminal, comprise micro-fluidic chip module 1, wireless drive control module 2 and there is the wireless mobile intelligent terminal 3 of wifi function; Micro-fluidic chip module is connected with wireless drive control module both-way communication, and wireless drive control module is connected with the wireless mobile intelligent terminal both-way communication with wifi function by wireless network.
Micro-fluidic chip module in the present invention comprises the micro-fluidic chip that does not contain pump, valve arrangement and the micro-fluidic chip that contains pump, valve arrangement.
Referring to Fig. 2, wireless drive control module 2 in the present invention comprises wifi chip 21, Bluetooth chip 22, infrared chip 23, single-chip microcomputer 24 and power supply 25, wifi chip, Bluetooth chip and infrared chip are connected with single-chip microcomputer two-way communication respectively, and power supply is connected and powers to single-chip microcomputer with monolithic.
The wireless mobile intelligent terminal with wifi function in the present invention is smart mobile phone, computer or intelligent MP4.
In the wireless mobile intelligent terminal with wifi function in the present invention, be provided with fluid and control software, by this fluid control software can control fluid in micro-fluidic chip flow with stop, the currency of fluid and the flow velocity of fluid.
By fluid, control software and can realize a wireless mobile intelligent terminal and control a plurality of micro-fluidic chips, or a plurality of wireless mobile intelligent terminal is shared a micro-fluidic chip.
Referring to Fig. 4, fluid in the present invention in wireless mobile intelligent terminal is controlled software, can by wifi control fluid in micro-fluidic chip flow with stop, the currency of fluid and the flow velocity of fluid, there are two kinds of mode of operations of the manual mode of standalone feature operation and the automatic mode of multiple standalone feature combination operation.
Below Application Example of the present invention:
The detection of the protein marker in embodiment 1 micro blood sample
Micro-fluidic chip is that (its chip design, manufacture method and experiment flow are shown in Fan R to double-deck PDMS chip, Vermesh O, SrivastavaA, et al. Nature Biotechnology2008,26,1373-1378), be a kind of can be to the chip that in blood samples of patients, multiple protein mark detects simultaneously, wherein lower floor is the fluidized bed of sample and reagent, and upper strata is valve layer, can control flowing and stopping of liquid in lower floor.This blood testing chip can be divided into two parts, a part is from micro whole blood, to isolate the sample pre-treatments part of blood plasma above, it can get rid of the interference that haemocyte is measured plasma proteins, rear portion is Antibody microarray, can to tens in micro-blood plasma even tens kinds of protein markers detect simultaneously.
In actual clinical detects, in order to make to detect, can complete fast, chip is used confining liquid (3% bovine serum albumin(BSA) BSA) sealing in advance, and builds Antibody microarray, is placed in 4 degree in wet box and preserves, and chip just can be at any time for patient like this.The Antibody microarray building in its chips is strips, wide 20 microns, 30 microns, adjacent ribbons interval, this group antibody is comprised of the band for 8 kinds of different protein markers such as prostate specific antigen (PSA), plasminogen (Plasminogen), c reactive protein (CRP), serum complement C3, tumor necrosis factor α (TNF-α), interleukin 10 (IL-10), interleukin-6 (IL-6), interleukin-1 betas (IL-1 β) respectively, repeats altogether 10 times.Capture antibody (being primary antibodie) is purchased from U.S. R & D Systems company and abcam company, the polynucleotides that are used for building Antibody microarray are by giving birth to work biosynthesis, build the method for Antibody microarray with reference to Fan R, Vermesh O, Srivastava A, et al.Nature Biotechnology2008,26,1373-1378.
In detection of clinical, with micro-hemostix, obtain blood samples of patients sample approximately 5 microlitres when patient blood sample, add anti-coagulants ethylenediamine tetra-acetic acid (EDTA) and be diluted to 10 microlitres, add chip, with the flow velocity of the 1 mul/min chip of flowing through, flow velocity with 5 mul/min passes into 1%BSA solution cleaning 2 minutes, add biotin labeled two anti-(purchased from U.S. R & D Systems company and abcam companies), flow velocity with 3 mul/min leads to 10 minutes, flow velocity with 5 mul/min passes into 1%BSA solution cleaning 1 minute again, the fluorescence molecule Cy5 (purchased from U.S. R & D Systems company) that adds Avidin mark, flow velocity with 5 mul/min leads to 5 minutes, flow velocity with 5 mul/min passes into PBS solution cleaning 2 minutes again, whole detection has accused, 30 minutes consuming time, and from patient blood sample collection to obtaining final result, whole process can complete in 1 hour.On the previous chip of order, can integrate 8 groups of such plasma proteins detection systems, can carry out the Parallel testing of 8 duplicate samples.
The structured flowchart of the portable medical device based on micro-fluidic chip as shown in Figure 3, wifi chip and single-chip microcomputer STM32 form wireless drive control module, after starting up, Micro Controller Unit (MCU) driving wifi chip sends wireless signal as WAP (wireless access point), and accept by SPI mouth the Iphone instruction that wifi chip is received, the break-make of a plurality of relays is controlled in single-chip microcomputer parallel port, the corresponding hydraulic valve of switch, each hydraulic valve connects different passages and liquid liquid storage tank, so can control different liquid, flow into and stop, completing successively the steps such as sample introduction, reaction and cleaning.This Medical Devices size is about 20X30X30 centimetre.
There is the Iphone of wifi radio function as mobile wireless control appliance, by wifi, be connected with the portable medical device of controlling micro-fluidic chip, can select as shown in Figure 4 automatic mode by the liquid flow order setting in advance and time (sample introduction react 10 minutes, clean 2 minutes, pass into biotin labeled two anti-10 minutes, clean 1 minute, the fluorescence molecule 5 minutes of Avidin mark, clean 2 minutes) start also operational outfit, also can manually control item by item and complete.After testing process completes, take off the sheet glass of micro-fluidic chip bottom, with chip scanner Genepix4400A (purchased from U.S. Molecular Devices company), scan and reading out data.
Claims (6)
1. the micro-fluidic chip liquid control system based on wireless mobile intelligent terminal, is characterized in that: comprise micro-fluidic chip module, wireless drive control module and have the wireless mobile intelligent terminal of wifi function; Micro-fluidic chip module is connected with wireless drive control module both-way communication, and wireless drive control module is connected with the wireless mobile intelligent terminal both-way communication with wifi function by wireless network.
2. the micro-fluidic chip liquid control system based on wireless mobile intelligent terminal as claimed in claim 1, is characterized in that: described micro-fluidic chip module comprises the micro-fluidic chip that does not contain pump, valve arrangement and the micro-fluidic chip that contains pump, valve arrangement.
3. the micro-fluidic chip liquid control system based on wireless mobile intelligent terminal as claimed in claim 1, it is characterized in that: described wireless drive control module comprises wifi chip, Bluetooth chip, infrared chip, single-chip microcomputer and power supply, wifi chip, Bluetooth chip and infrared chip are connected with single-chip microcomputer two-way communication respectively, and power supply is connected and powers to single-chip microcomputer with monolithic.
4. the micro-fluidic chip liquid control system based on wireless mobile intelligent terminal as claimed in claim 1, is characterized in that: described in there is wifi function wireless mobile intelligent terminal be smart mobile phone, panel computer or intelligent MP4.
5. the micro-fluidic chip liquid control system based on wireless mobile intelligent terminal as claimed in claim 1, it is characterized in that: described in have and in the wireless mobile intelligent terminal of wifi function, be provided with fluid and control software, by this fluid control software can control fluid in micro-fluidic chip flow with stop, the currency of fluid and the flow velocity of fluid.
6. the micro-fluidic chip liquid control system based on wireless mobile intelligent terminal as claimed in claim 5, it is characterized in that: by fluid, control software and can realize a wireless mobile intelligent terminal and control a plurality of micro-fluidic chips, or a plurality of wireless mobile intelligent terminal is shared a micro-fluidic chip.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310547782.6A CN103595864A (en) | 2013-11-06 | 2013-11-06 | Microfluidic chip liquid control system based on wireless mobile intelligent terminal |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310547782.6A CN103595864A (en) | 2013-11-06 | 2013-11-06 | Microfluidic chip liquid control system based on wireless mobile intelligent terminal |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103595864A true CN103595864A (en) | 2014-02-19 |
Family
ID=50085836
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310547782.6A Pending CN103595864A (en) | 2013-11-06 | 2013-11-06 | Microfluidic chip liquid control system based on wireless mobile intelligent terminal |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103595864A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104849484A (en) * | 2015-05-27 | 2015-08-19 | 胡师彦 | Control system of biochip |
| CN105259934A (en) * | 2015-11-02 | 2016-01-20 | 哈尔滨工业大学 | Wireless remote control micro-injection device |
| CN105319380A (en) * | 2015-10-24 | 2016-02-10 | 哈尔滨工业大学 | Wireless pneumatic/hydraulic microfluidic module |
| CN108614100A (en) * | 2018-07-19 | 2018-10-02 | 苏州芯海智能科技有限公司 | A kind of quickly and easily detection device |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101576557A (en) * | 2008-05-07 | 2009-11-11 | 中国科学院电子学研究所 | Integrated micro-fluidic chip system |
| CN103091486A (en) * | 2011-11-01 | 2013-05-08 | 马义才 | Test strip detection system |
| CN103197086A (en) * | 2013-04-13 | 2013-07-10 | 西安科技大学 | Microfluidic underground coal mine air monitoring system and method |
| CN103274352A (en) * | 2013-05-23 | 2013-09-04 | 安徽海聚信息科技有限责任公司 | MEMS module and control system based on same |
| CN103308502A (en) * | 2013-06-01 | 2013-09-18 | 浙江大学 | Handheld general microfluidic chip real-time detection device and application |
-
2013
- 2013-11-06 CN CN201310547782.6A patent/CN103595864A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101576557A (en) * | 2008-05-07 | 2009-11-11 | 中国科学院电子学研究所 | Integrated micro-fluidic chip system |
| CN103091486A (en) * | 2011-11-01 | 2013-05-08 | 马义才 | Test strip detection system |
| CN103197086A (en) * | 2013-04-13 | 2013-07-10 | 西安科技大学 | Microfluidic underground coal mine air monitoring system and method |
| CN103274352A (en) * | 2013-05-23 | 2013-09-04 | 安徽海聚信息科技有限责任公司 | MEMS module and control system based on same |
| CN103308502A (en) * | 2013-06-01 | 2013-09-18 | 浙江大学 | Handheld general microfluidic chip real-time detection device and application |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104849484A (en) * | 2015-05-27 | 2015-08-19 | 胡师彦 | Control system of biochip |
| CN104849484B (en) * | 2015-05-27 | 2016-10-26 | 谢俊 | The control system of biochip |
| CN105319380A (en) * | 2015-10-24 | 2016-02-10 | 哈尔滨工业大学 | Wireless pneumatic/hydraulic microfluidic module |
| CN105259934A (en) * | 2015-11-02 | 2016-01-20 | 哈尔滨工业大学 | Wireless remote control micro-injection device |
| CN105259934B (en) * | 2015-11-02 | 2018-06-26 | 哈尔滨工业大学 | Wireless remote control microinjection apparatus |
| CN108614100A (en) * | 2018-07-19 | 2018-10-02 | 苏州芯海智能科技有限公司 | A kind of quickly and easily detection device |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN109647553B (en) | Multi-index disease joint detection microfluidic device | |
| CN101576557B (en) | Integrated micro-fluidic chip system | |
| CN100503838C (en) | Microflow system for analyzing nucleic acid | |
| EP0865606B1 (en) | Devices and methods for using centripetal acceleration to drive fluid movement in a microfluidics system with on-board informatics | |
| US20010055812A1 (en) | Devices and method for using centripetal acceleration to drive fluid movement in a microfluidics system with on-board informatics | |
| CN108535239A (en) | Micro-fluidic chip based on microlayer model and detecting system | |
| CN103595864A (en) | Microfluidic chip liquid control system based on wireless mobile intelligent terminal | |
| US20050069913A1 (en) | Devices and methods for using centripetal acceleration to drive fluid movement in a microfluidics system | |
| CN101184983A (en) | Methods and device for transmitting, enclosing and analysing fluid samples | |
| US20170095810A1 (en) | Handheld fluid handling systems and methods | |
| DK0871888T3 (en) | Automated Molecular Biological Diagnosis System | |
| CN106140334B (en) | Micro-fluidic chip detection reaction unit with reagent store function | |
| CN101073002A (en) | Microfluidic devices | |
| US10596566B2 (en) | Capillary-action microfluidic device for point-of-care diagnostics | |
| JP2019533808A (en) | Analysis system and method for inspecting a sample | |
| Mitsakakis et al. | Diagnostic tools for tackling febrile illness and enhancing patient management | |
| CN211463197U (en) | Microfluid detection chip adopting immune electrode | |
| CN105628660B (en) | A kind of passive micro-valve POCT chips | |
| US10702867B2 (en) | Microfluidics sensing system | |
| CN102998472A (en) | Automatic device for heterogeneous immunization rapid analysis and use method of automatic device | |
| He et al. | Microfluidic technology for multiple single-cell capture | |
| CN100504389C (en) | Transmission through applied microflow liquid, and method of analyzing system | |
| WO2021108266A1 (en) | Systems and devices for sample preparation and analyte detection | |
| Novak et al. | Microfluidic platform for multiplexed cell sampling and time-resolved spr-based cytokine sensing | |
| KR101044786B1 (en) | Flow controller for bio chip |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20140219 |