CN103592433A - Point arranging method for biochip microarray - Google Patents
Point arranging method for biochip microarray Download PDFInfo
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- CN103592433A CN103592433A CN201310626472.3A CN201310626472A CN103592433A CN 103592433 A CN103592433 A CN 103592433A CN 201310626472 A CN201310626472 A CN 201310626472A CN 103592433 A CN103592433 A CN 103592433A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54306—Solid-phase reaction mechanisms
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Abstract
The invention provides a point arranging method for a biochip microarray. Array points of the micro-array is circular or rectangular; sizes of the array points and distance between centers of every two adjacent array points are controlled according to difference of biochip substrates so as to fully utilize arrayable space of a biochip, reduce waste of space and cross contamination among the array points of the biochip and improve effectiveness of array signals of the biochip.
Description
Technical field
The present invention relates to biological immune technical field, particularly a kind of biochip microarray points distributing method.
Background technology
The develop rapidly of science and technology is promoting the progress in the fields such as life science, biotechnology research, drug development and research, biochip is with advantages such as its high density, high flux, many target spots, is applied to more and more the various aspects such as experiment, production, manufacture of life science.
Existing biochip (Bio-chip) refers to the height being coated on solid phase carrier, in, low-density nucleic acid (cDNA, mRNA) probe, protein (polypeptide, enzyme molecule, antigen, antibody), medicine or acceptor, the microarray of cell or tissue (Microarray), biochip utilize between biomacromolecule, have special mutual identification ability and by their the orderly solid phase carrier substrate (film that is arranged in, glass sheet, silicon chip, ceramics etc.) on, carry out specific reaction or hybridization with sample to be checked and corresponding mark biomolecule simultaneously, by automatic reading equipment, can obtain a large amount of useful life science information.
Therefore, in biochip, can be furnished with a plurality of array points simultaneously.Yet array point layout can cause the problems such as phase mutual interference between chip space waste, array point, array signal validity be poor irrelevantly.Be embodied in, if the array point of arranging on biochip is very few, cause the waste of biochip host material space, and the setting of array point be too intensive, can cause again the phase mutual interference between array point, the distorted signals that biochip is produced.
Summary of the invention
Technical matters to be solved: the invention provides a kind of biochip microarray points distributing method for above problem, the microarray of reasonable Arrangement biochip of the present invention is layouted, according to the difference of biochip host material, control OC relation between array point size and adjacent array point, both made full use of the space that biochip can be structured the formation, reduce space waste, reduce again the cross pollution between biochip array point, improve the validity of biochip array signal.
Technical scheme: in order to overcome the above problems, the invention provides a kind of biochip microarray points distributing method, described array point is circular array point or rectangular array point, d is diameter or the rectangular array point length of side of circular array point, R is the center distance between adjacent array point, x is the minor increment of adjacent array point back gauge, meets formula R=d+x, x >=20%*d.
Described microarray is the capable dot matrix of m row n, and wherein m and n are natural number, the multiple that wherein m is 3.
Beneficial effect: different according to biochip host material, the microarray of reasonable Arrangement biochip is layouted, by adjusting OC relation between array point size and array point, both made full use of the space that biochip can be structured the formation, reduce space waste, reduce again the cross pollution between biochip array point, improve the validity of biochip array signal.
Accompanying drawing explanation
Fig. 1 is the structural representation of biological detection chip in the present invention.
Fig. 2 is the partial enlarged drawing of the biochip of embodiment 1 in the present invention.
Fig. 3 is the circular array dot structure schematic diagram of embodiment 1 in the present invention.
Fig. 4 is the partial enlarged drawing of the biochip of embodiment 2 in the present invention.
Fig. 5 is the rectangular array dot structure schematic diagram of embodiment 2 in the present invention.
Embodiment
Below in conjunction with Figure of description, the present invention is described in further detail.
Embodiment 1
As shown in Figures 1 to 3, a kind of biochip microarray points distributing method, described array point is circular array point, d is the diameter of circular array point, and R is the center distance between adjacent array point, and x is the minor increment of adjacent array point back gauge, meet formula R=d+x, and meet x >=20%*d.
Described microarray is the capable dot matrix of m row n, and wherein m and n are natural number, the multiple that wherein m is 3.
Embodiment 2
As shown in Fig. 1,4,5, a kind of biochip microarray points distributing method, described array point is rectangular array point, d is the rectangular array point length of side, and R is the center distance between adjacent array point, and x is the minor increment of adjacent array point back gauge, meet formula R=d+x, and x >=20%*d.
Described microarray is the capable dot matrix of m row n, and wherein m and n are natural number, the multiple that wherein m is 3.
Claims (3)
1. a biochip microarray points distributing method, it is characterized in that: described array point is circular array point or rectangular array point, d is diameter or the rectangular array point length of side of circular array point, R is the center distance between adjacent array point, x is the minor increment of adjacent array point back gauge, meet formula R=d+x, x >=20%*d.
2. a kind of biochip microarray points distributing method according to claim 1, is characterized in that: described microarray is the capable dot matrix of m row n, and wherein m and n are natural number.
3. a kind of biochip microarray points distributing method according to claim 2, is characterized in that: the multiple that m is 3.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310626472.3A CN103592433A (en) | 2013-11-29 | 2013-11-29 | Point arranging method for biochip microarray |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310626472.3A CN103592433A (en) | 2013-11-29 | 2013-11-29 | Point arranging method for biochip microarray |
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| CN103592433A true CN103592433A (en) | 2014-02-19 |
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| CN201310626472.3A Pending CN103592433A (en) | 2013-11-29 | 2013-11-29 | Point arranging method for biochip microarray |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1548444A1 (en) * | 2003-12-23 | 2005-06-29 | Paul Scherrer Institut | An assay chip, and uses of said assay chip to determine molecular structures and functions |
| EP1958689A1 (en) * | 2007-02-13 | 2008-08-20 | Samsung Electronics Co., Ltd. | Microarray and method of producing the same |
| CN101285822A (en) * | 2007-04-13 | 2008-10-15 | 上海芯超生物科技有限公司 | Tumor tissue micro- array tissue chip |
| CN102735839A (en) * | 2011-04-13 | 2012-10-17 | 上海芯超生物科技有限公司 | Staged and graded microarray tissue chip for research on esophageal cancer tissue metastasis suppressor gene nm23-H1 |
| CN103257233A (en) * | 2013-05-31 | 2013-08-21 | 南京祥中生物科技有限公司 | Biochip and method capable of simultaneously visually detecting multiple antibiotics, illegal addition agents and biotoxins |
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2013
- 2013-11-29 CN CN201310626472.3A patent/CN103592433A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1548444A1 (en) * | 2003-12-23 | 2005-06-29 | Paul Scherrer Institut | An assay chip, and uses of said assay chip to determine molecular structures and functions |
| EP1958689A1 (en) * | 2007-02-13 | 2008-08-20 | Samsung Electronics Co., Ltd. | Microarray and method of producing the same |
| CN101285822A (en) * | 2007-04-13 | 2008-10-15 | 上海芯超生物科技有限公司 | Tumor tissue micro- array tissue chip |
| CN102735839A (en) * | 2011-04-13 | 2012-10-17 | 上海芯超生物科技有限公司 | Staged and graded microarray tissue chip for research on esophageal cancer tissue metastasis suppressor gene nm23-H1 |
| CN103257233A (en) * | 2013-05-31 | 2013-08-21 | 南京祥中生物科技有限公司 | Biochip and method capable of simultaneously visually detecting multiple antibiotics, illegal addition agents and biotoxins |
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Application publication date: 20140219 |