CN103585787A - Application of suspension isolation balls in separation gel blood collection tube - Google Patents
Application of suspension isolation balls in separation gel blood collection tube Download PDFInfo
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- CN103585787A CN103585787A CN201310571186.1A CN201310571186A CN103585787A CN 103585787 A CN103585787 A CN 103585787A CN 201310571186 A CN201310571186 A CN 201310571186A CN 103585787 A CN103585787 A CN 103585787A
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Abstract
The invention provides an application of suspension isolation balls in a separation gel blood collection tube, belongs to the field of blood collection tubes and solves the problem that secondary blood collection is caused due to the fact that separation gel micelles float in serum during separation of the serum and blood plasma. A plurality of the suspension isolation balls form an isolation belt and are arranged above the separation gel, and the proportion of the suspension isolation balls is larger than that of the serum and smaller than that of the blood plasma at the same time, so that the suspension isolation balls can float above the separation gel, and serum collection of a probe is not affected; particle sizes of the suspension isolation balls are controlled to be between 1.0 mm and 2.5 mm, so that the serum can pass through seams of the isolation balls freely; and free separation gel is restrained in a lower layer due to a stacking effect of the suspension isolation balls and cannot pass through the isolation belt formed by the suspension isolation balls.
Description
Technical field
The invention belongs to heparin tube field, the application of batching sphere on separation gel heparin tube is specifically related to suspend.
Background technology
In clinical examination, the blood sample for Clinical laboratory tests such as biochemical indicator, amynologic index, coagulation function analysis, detection of nucleic acids, need to give separated by the serum in whole blood and blood plasma.As one of separated method, have in the heparin tube of ratio heavy substance that has been equipped with the centre with serum and plasma and take whole blood sample, by centrifugation, operate the centre that makes this material be positioned at serum and plasma, thereby carry out separation.Adopt the method, can not operate because of pipette, decant etc. sneaked into blood cell composition in serum.
So far, as the parting material of serum or blood plasma, main use has thixotropic gel-like material, for example, have alpha-olefin-maleic acid diester copolymer of particular viscosity scope, and proportion adjustable range is 1.035～1.055.
But, utilize in the separation process of the serum and plasma that gel carries out, because gels-soft, inner difference of specific gravity, a macromolecule ball of string are shunk and reasons such as vibration during sample pretreating, have separation gel micelle dissociates out from body, float among serum, not yet having at present any brand separation gel can stop this phenomenon completely occurs, also without any heparin tube producer, for this phenomenon, do special safeguard procedures, the secondary blood sampling that produced has thus increased patient's misery and medical treatment cost, has also increased the detection burden of hospital simultaneously.
Summary of the invention
The object of the invention is in order to solve in existing serum and plasma separation, separation gel micelle floats in serum, causes the problem of secondary blood sampling, and the application of suspension batching sphere on separation gel heparin tube is provided.
The invention provides a kind of application of batching sphere on separation gel heparin tube that suspend, the proportion that the proportion of described suspension batching sphere is greater than serum is less than the proportion of separation gel simultaneously.
Preferably, described separation gel proportion is 1.055, and suspension batching sphere proportion is 1.045-1.054.
Preferably, described suspension batching sphere be take resin and proportion conditioning agent and is prepared as raw material.
Preferably, described resin comprises polyvinyl resin, acrylic resin, polyester, polyurethane, silicon rubber or fluoroplastics.
Preferably, described proportion conditioning agent comprises kaolin, talcum powder, silica, mica powder or magnetic.
Preferably, the particle diameter of described suspension batching sphere is 1.0mm-2.5mm.
Beneficial effect of the present invention
The invention provides the application of suspension batching sphere on separation gel heparin tube, several described suspension batching spheres form isolation strip, be arranged on the top of separation gel, the proportion that the proportion of suspension batching sphere is greater than serum is less than the proportion of insulation rubber simultaneously, suspension batching sphere can be suspended in the top of separation gel like this, but do not affect the collection of probe to serum, the particle diameter of suspension batching sphere is controlled between 1.0mm-2.5mm, to guarantee that serum can freely pass through in the gap of batching sphere, free separation gel is because the pile up effect of suspension batching sphere is suppressed in lower floor, the isolation strip that cannot form by suspension batching sphere.
Figure of description
Fig. 1 is the schematic diagram of separation gel heparin tube of the present invention;
Fig. 2 is the suspend structural representation of batching sphere of the present invention.
In figure, 1, seal cover, 2, serum, 3, suspension batching sphere, 4, separation gel, 5, blood plasma, 6, resin matrix, 7, proportion conditioning agent.
The specific embodiment
In order further to understand the present invention, below in conjunction with embodiment, the preferred embodiments of the invention are described, but should be appreciated that these are described is the restriction for further illustrating the features and advantages of the present invention rather than patent of the present invention being required.
The invention provides a kind of application of batching sphere on separation gel heparin tube that suspend, the proportion that the proportion of described suspension batching sphere is greater than serum is less than the proportion of separation gel simultaneously.
Separation gel proportion of the present invention is slightly different according to different preparation technologies, can to suspension batching sphere proportion, adjust according to actual conditions.Preferably, described separation gel proportion is 1.055, and suspension batching sphere proportion is 1.045-1.054.
Suspension batching sphere of the present invention is comprised of resin 6 and proportion conditioning agent 7, as shown in Figure 2, first according to the requirement of suspension batching sphere proportion, take resin and proportion conditioning agent, after high-speed mixer evenly mixes, at 180～200 ℃ of double-screw extruding pelletizings, after oven dry, obtain the batching sphere that suspends.The particle diameter of described suspension batching sphere is preferably 1.0mm-2.5mm, and suspension batching sphere particle diameter is less, preferably adopts underwater pellet cutting system granulation.
Resin of the present invention is not particularly limited, and adopts medical rank and non-water-soluble resin, preferably includes polyvinyl resin, acrylic resin, polyester, polyurethane, silicon rubber or fluoroplastics, more preferably polyvinyl resin or acrylic resin.
Proportion conditioning agent of the present invention comprises kaolin, talcum powder, silica, mica powder or magnetic.
Resin of the present invention and proportion conditioning agent, according to parts by weight meter, comprise 100 parts of resins, proportion conditioning agent 8.9-17.8 part.
Separation gel heparin tube schematic diagram of the present invention as shown in Figure 1, wherein several suspension batching spheres 3 form isolation strip, be arranged on the top of separation gel 4, the proportion that the proportion of suspension batching sphere 3 is greater than serum 2 is less than the proportion of insulation rubber 4 simultaneously, suspension batching sphere 3 can be suspended in the top of separation gel 4 like this, but do not affect the collection of probe to serum, the particle diameter of suspension batching sphere 3 is controlled between 1.0mm-2.5mm, to guarantee that serum 3 can freely pass through in the gap of batching sphere, free separation gel 4 is because the pile up effect of suspension batching sphere 3 is suppressed in lower floor, the isolation strip that cannot form by suspension batching sphere 3.It should be noted that controlling batching sphere size is slightly larger than probe internal diameter size and prevents mistake suction simultaneously.
Separation gel heparin tube of the present invention is manufactured according to the production technology of prior art, through blank pipe injecting glue, centrifugal, vacuumizes, organizes cap, last overall package.Described suspension batching sphere does not change existing separation gel heparin tube production technology, only need after rotary process completes, directly in pipe, add scheduled volume batching sphere, follow-up vacuumize, organize cap operation and technological parameter constant, technique is simple.
Below in conjunction with embodiment, the invention will be further described.
Embodiment 1
100 parts of acrylic resins and 15.4 parts of modified kaolins are put in high-speed mixer and mixed, compound is extruded through double screw extruder, underwater pellet cutting system granulation, obtains the batching sphere that suspends after oven dry, the particle diameter of described suspension batching sphere is 1.5mm.The effect that the suspension batching sphere that embodiment 1 obtains is applied on separation gel heparin tube is as shown in table 1.
100 parts of acrylic resins and 17.8 parts of modified kaolins are put in high-speed mixer and mixed, compound is extruded through double screw extruder, underwater pellet cutting system granulation, obtains the batching sphere that suspends after oven dry, the particle diameter of described suspension batching sphere is 1.5mm.The effect that the suspension batching sphere that embodiment 2 obtains is applied on separation gel heparin tube is as shown in table 1.
100 parts of acrylic resins and 9.8 parts of modified kaolins are put in high-speed mixer and mixed, compound is extruded through double screw extruder, underwater pellet cutting system granulation, obtains the batching sphere that suspends after oven dry, and the particle diameter of described suspension batching sphere is 1.5mm.The effect that the suspension batching sphere that embodiment 3 obtains is applied on separation gel heparin tube is as shown in table 1.
100 parts of acrylic resins and 8.9 parts of modified kaolins are put in high-speed mixer and mixed, compound is extruded through double screw extruder, underwater pellet cutting system granulation, obtains the batching sphere that suspends after oven dry, and the particle diameter of described suspension batching sphere is 1.5mm.The effect that the suspension batching sphere that embodiment 4 obtains is applied on separation gel heparin tube is as shown in table 1.
Table 1 batching sphere effect comparison table
Note: 2,000 every group finished product separation gel heparin tubes, copper-bath simulation blood sampling test, control group adopts the separation gel heparin tube that does not add suspension batching sphere.
40 upset experiments:
A) being ready to proportion is 1.040 copper sulphate standard liquid;
B) in clean test tube (do not add the separation gel heparin tube of suspension batching sphere or add the separation gel heparin tube of the suspension batching sphere of embodiment 1-4) bottom, add separation gel 1g, on centrifuge with the speed of 3500r/min after centrifugal 3 minutes, add copper sulphate standard liquid, the liquid level of standard liquid is 1/3rd of test tube length apart from the length of test tube mouth;
C) test tube that adds standard liquid is put into 40 ℃ of heating water baths of thermostat 2 hours;
D) on centrifuge with the speed of 3200r/min centrifugal 5 minutes.Sample quantities must not be lower than 10, if having one or an above sample to overturn completely, two or two above partial switchings, be all judged to be defective.
80 upset experiments:
A) being ready to proportion is 1.080 copper sulphate standard liquid;
B) in clean test tube (do not add the separation gel heparin tube of suspension batching sphere or add the separation gel heparin tube of the suspension batching sphere of embodiment 1-4) bottom, add separation gel 1g, on centrifuge with the speed of 3500r/min after centrifugal 3 minutes, add copper sulphate standard liquid, the liquid level of standard liquid is 1/3rd of test tube length apart from the length of test tube mouth;
C) test tube that adds standard liquid being put into 4 ℃ of refrigerating chambers deposits 2 hours;
D) on centrifuge with the speed of 3200r/min centrifugal 5 minutes.Sample quantities must not be lower than 10, if having one or an above sample not overturn completely, two or two above partial switchings, be all judged to be defective.
As can be seen from Table 1, show after tested, suspension batching sphere, when not affecting separation gel upset performance, can effectively prevent that separation gel from floating the generation of glue phenomenon, closely stops probe and stops up.
The explanation of above embodiment is just for helping to understand method of the present invention and core concept thereof.It should be pointed out that for those skilled in the art, under the premise without departing from the principles of the invention, can also carry out some improvement and modification to the present invention, these improvement and modification also fall in the protection domain of the claims in the present invention.
Above-mentioned explanation to the disclosed embodiments, makes professional and technical personnel in the field can realize or use the present invention.To the multiple modification of these embodiment, will be apparent for those skilled in the art, General Principle as defined herein can, in the situation that not departing from the spirit or scope of the present invention, realize in other embodiments.Therefore, the present invention will can not be restricted to these embodiment shown in this article, but will meet the widest scope consistent with principle disclosed herein and features of novelty.
Claims (6)
1. the application of suspension batching sphere on separation gel heparin tube, is characterized in that, the proportion that the proportion of described suspension batching sphere is greater than serum is less than the proportion of separation gel simultaneously.
2. the application of suspension batching sphere according to claim 1 on separation gel heparin tube, is characterized in that, described separation gel proportion is 1.055, and suspension batching sphere proportion is 1.045-1.054.
3. the application of suspension batching sphere according to claim 1 on separation gel heparin tube, is characterized in that, described suspension batching sphere be take resin and proportion conditioning agent and prepared as raw material.
4. the application of suspension batching sphere according to claim 3 on separation gel heparin tube, is characterized in that, described resin comprises polyvinyl resin, acrylic resin, polyester, polyurethane, silicon rubber or fluoroplastics.
5. the application of suspension batching sphere according to claim 3 on separation gel heparin tube, is characterized in that, described proportion conditioning agent comprises kaolin, talcum powder, silica, mica powder or magnetic.
6. the application of the suspension batching sphere described in any one on separation gel heparin tube according to claim 1-3, is characterized in that, the particle diameter of described suspension batching sphere is 1.0mm-2.5mm.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310571186.1A CN103585787A (en) | 2013-11-15 | 2013-11-15 | Application of suspension isolation balls in separation gel blood collection tube |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201310571186.1A CN103585787A (en) | 2013-11-15 | 2013-11-15 | Application of suspension isolation balls in separation gel blood collection tube |
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS58218651A (en) * | 1982-06-14 | 1983-12-19 | Nippon Paint Co Ltd | Blood separation device |
| JPH0599917A (en) * | 1991-10-08 | 1993-04-23 | Terumo Corp | Blood separating tube |
| CN1096373A (en) * | 1993-06-09 | 1994-12-14 | 中国科学院大连化学物理研究所 | The preparation method of a kind of serum separation gel and heparin tube |
| CN1281145A (en) * | 2000-08-08 | 2001-01-24 | 湖北医科大学 | Blood separating colloid |
| JP2007271388A (en) * | 2006-03-30 | 2007-10-18 | Hidetoshi Tsuchida | Separation method of serum or plasma, and blood separation tube |
| CN102209895A (en) * | 2008-11-07 | 2011-10-05 | 日立化成工业株式会社 | Blood serum or blood plasma separating material and blood-collecting tube using same |
-
2013
- 2013-11-15 CN CN201310571186.1A patent/CN103585787A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS58218651A (en) * | 1982-06-14 | 1983-12-19 | Nippon Paint Co Ltd | Blood separation device |
| JPH0599917A (en) * | 1991-10-08 | 1993-04-23 | Terumo Corp | Blood separating tube |
| CN1096373A (en) * | 1993-06-09 | 1994-12-14 | 中国科学院大连化学物理研究所 | The preparation method of a kind of serum separation gel and heparin tube |
| CN1281145A (en) * | 2000-08-08 | 2001-01-24 | 湖北医科大学 | Blood separating colloid |
| JP2007271388A (en) * | 2006-03-30 | 2007-10-18 | Hidetoshi Tsuchida | Separation method of serum or plasma, and blood separation tube |
| CN102209895A (en) * | 2008-11-07 | 2011-10-05 | 日立化成工业株式会社 | Blood serum or blood plasma separating material and blood-collecting tube using same |
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Application publication date: 20140219 |