CN103571748A - Microbial electrochemical system for continuous culture, sampling and real-time detection - Google Patents

Microbial electrochemical system for continuous culture, sampling and real-time detection Download PDF

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Publication number
CN103571748A
CN103571748A CN201310467369.9A CN201310467369A CN103571748A CN 103571748 A CN103571748 A CN 103571748A CN 201310467369 A CN201310467369 A CN 201310467369A CN 103571748 A CN103571748 A CN 103571748A
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China
Prior art keywords
sampling
real
reactor
electrochemical system
culture
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CN201310467369.9A
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Chinese (zh)
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张宇
李介夫
肖湘
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Shanghai Jiaotong University
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Shanghai Jiaotong University
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Priority to CN201310467369.9A priority Critical patent/CN103571748A/en
Publication of CN103571748A publication Critical patent/CN103571748A/en
Priority to CN201410187030.8A priority patent/CN104004655A/en
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/58—Reaction vessels connected in series or in parallel
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/20—Degassing; Venting; Bubble traps
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/02—Electrical or electromagnetic means, e.g. for electroporation or for cell fusion
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
    • C12M37/04—Seals
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • C12M41/14—Incubators; Climatic chambers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/48—Automatic or computerized control

Abstract

The invention provides a microbial electrochemical system for continuous culture, sampling and real-time detection. The microbial electrochemical system comprises a culture medium container, a peristaltic pump, a culture reactor, a gas cabinet, an exhaust and ventilation system, a computer and an electrochemical workstation. The microbial electrochemical system has the beneficial effects that the system comprises three pairs of parallel culture reaction chambers, and completely parallel contrast can be realized in electrical stimulation tests; continuous culture can be independently performed in the six culture reaction chambers, and different flow velocities and different electrode surface shear forces can be provided; gas-liquid closeness and chemical and biological pollution isolation are kept for a long time; culture solution sampling and electrode surface sampling are realized in an experimental process; with widely adaptive interfaces and modular design, a specific electrode or other parts can be conveniently transformed and loaded according to experimental requirements; the microbial electrochemical system mainly aims at microbes from ocean and is suitable for analysis of multiple biological samples comprising environmental samples and pure culture samples.

Description

For cultured continuously, sampling and the real-time microorganism electrochemical system detecting
Technical field
The present invention relates to microorganism electrochemical technology and culture technique field, refer to especially a kind of microorganism electrochemical system for cultured continuously, sampling and real-time detection for Deep-Sea Microorganisms research.
Background technology
Microbiological fuel cell technology can become electricity collection by the contained energy transformation of organism in sewage, and utilize microorganism electrochemical reaction development of fuel cells, dispose of sewage etc. has great potential; Meanwhile, adopt electrochemical method microorganisms metabolic process and microorganism-environment as mechanism, to be subject to extensive concern mutually; On the other hand, ocean, as understanding still shallow natural resources storehouse, has huge research and development potentiality, and the research of marine-derived microorganism is for explaining that microorganism-environment is made mechanism mutually, development of new natural resources has vital role.Yet, so far delivered or disclosed equipment scheme is studied based on traditional chemical mostly, research range is more single, be difficult to be applicable to the integration requirement to functions such as sampling and real-time parameter detections in polymorphic type biological specimen suitability, cultured continuously, experiment in the researchs such as microorganism enrichment of marine source, become the major obstacles in marine-derived microorganism electrochemical research and application.
Summary of the invention
For defect of the prior art, the technical problem to be solved in the present invention is to provide a kind of microorganism electrochemical system for cultured continuously, sampling and real-time detection for Deep-Sea Microorganisms research, in realizing cultured continuously, experiment in the multitask situation such as sampling, pollution-free in maintain system, can be according to different experiments, change the assemblies such as required electrode, in cultured continuously, real-time parameter detection etc., not interrupt experiments in the situation that, carry out nutrient solution sampling or electrode surface sampling.
The invention provides a kind of microorganism electrochemical system for cultured continuously, sampling and real-time detection, comprise medium container, peristaltic pump, cultivation reactor, gas cupboard, take out air exchange system, computer, electrochemical workstation;
Described medium container is for providing substratum by peristaltic pump for cultivating reactor; Described gas cupboard is for providing gas by taking out air exchange system for cultivating reactor; Described computer is for input control order; Described electrochemical workstation is for detecting in real time the data parameters of cultivating reactor according to the steering order of computer input.
Preferably, described electrochemical workstation is also for inputting electricity irritation according to the steering order of computer input to cultivating reactor.
Preferably, described computer is also for the detected data parameters of store electricity chem workstation.
Preferably, also comprise sampling system, wherein, described sampling system connects described cultivation reactor, and described sampling system is cultivated the culture of reactor for sampling.
Preferably, also comprise constant incubator, wherein, described cultivation reactor is arranged in described constant incubator, states cultivation reactor and is used to cultivation reactor to provide temperature adjustable environment.
Preferably, described cultivation reactor comprises exchange membrane, synthetic glass groove, silicone rubber O-ring, high pure graphite electrode, silicone rubber seal gasket, poly (methyl methacrylate) plate, screw, wherein, the both sides of described exchange membrane are symmetrical structure by screw respectively from inside to outside and fix successively organic glass guide channel, silicone rubber O-ring, high pure graphite electrode, silicone rubber seal gasket and poly (methyl methacrylate) plate.
Preferably, described cultivation reactor comprises multipair parallelly cultivate reaction chamber.
Preferably, described cultivation reaction chamber is for can independently carrying out the cultivation reaction chamber of cultured continuously.
Compared with prior art, the present invention has following beneficial effect:
1, contain three pairs of parallelly cultivate reaction chambers, in the experiment of electricity irritation class, can realize complete parallel contrast;
2, six cultivation reaction chambers can independently carry out cultured continuously, and different velocity of flow and Different electrodes surface shear power are provided;
3, keep for a long time gas-liquid closed and isolated chemistry and biological pollution;
4, realize sampling and electrode surface sampling in experimentation;
5, the interface and the modular design that extensively adapt to, reequip, install additional specific electrode or miscellaneous part according to experiment demand easily;
6,, mainly for the microorganism in Yu Haiyang source, be applicable to comprise the multiple biological sample analysis of environmental samples, pure culture sample.
Accompanying drawing explanation
By reading the detailed description of non-limiting example being done with reference to the following drawings, it is more obvious that other features, objects and advantages of the present invention will become:
Fig. 1 is functional block diagram of the present invention.
Fig. 2 cultivates the structural representation of reactor in Fig. 1.
Embodiment
Below in conjunction with specific embodiment, the present invention is described in detail.Following examples will contribute to those skilled in the art further to understand the present invention, but not limit in any form the present invention.It should be pointed out that to those skilled in the art, without departing from the inventive concept of the premise, can also make some distortion and improvement.These all belong to protection scope of the present invention.
The present invention is directed to the deficiencies in the prior art a kind of microorganism electrochemical system for cultured continuously, sampling and real-time detection for Deep-Sea Microorganisms research is provided, as shown in Figure 1, comprise medium container, peristaltic pump, cultivation reactor, gas cupboard, take out air exchange system, computer and electrochemical workstation, described medium container is for providing substratum by peristaltic pump for cultivating reactor; Described gas cupboard is for providing gas by taking out air exchange system for cultivating reactor; Described computer is for input control order; Described electrochemical workstation is for detecting in real time the data parameters of cultivating reactor according to the steering order of computer input; Described electrochemical workstation is also for inputting electricity irritation according to the steering order of computer input for cultivating reactor; Described computer is also for the detected data parameters of store electricity chem workstation.
Described cultivation reactor is also connected with the sampling system of the culture of cultivating reactor for sampling.
Described cultivation reactor is also provided with to be outward used to cultivates the constant incubator that reactor provides the adjustable environment of temperature.
As shown in Figure 2, described cultivation reactor comprises an exchange membrane 7, two synthetic glass grooves 6, two silicone rubber O-rings 5, two high pure graphite electrodes 4, two silicone rubber seal gaskets 3, two poly (methyl methacrylate) plates 2 and plus screw 1, described plus screw is the fixing reactor surrounding of cultivating respectively, and the both sides of described exchange membrane are symmetrical structure by screw respectively from inside to outside and fix successively organic glass guide channel, silicone rubber O-ring, high pure graphite electrode, silicone rubber seal gasket and poly (methyl methacrylate) plate.
In a preferred embodiment, the cultivation reactor of microorganism electrochemical system provided by the invention contains three pairs of parallelly cultivate reaction chambers, in the experiment of electricity irritation class, can realize complete parallel contrast; Six cultivation reaction chambers can independently carry out cultured continuously, and different velocity of flow and Different electrodes surface shear power are provided; Keep for a long time gas-liquid closed and isolated chemistry and biological pollution; Realize sampling and electrode surface sampling in experimentation; The interface and the modular design that extensively adapt to, reequip, install additional specific electrode or miscellaneous part according to experiment demand easily; For the microorganism of marine source, comprise the multiple biological sample analysis of environmental samples, pure culture sample.
Above specific embodiments of the invention are described.It will be appreciated that, the present invention is not limited to above-mentioned particular implementation, and those skilled in the art can make various distortion or modification within the scope of the claims, and this does not affect flesh and blood of the present invention.

Claims (8)

1. for cultured continuously, sampling and the real-time microorganism electrochemical system detecting, it is characterized in that, comprise medium container, peristaltic pump, cultivation reactor, gas cupboard, take out air exchange system, computer, electrochemical workstation;
Described medium container is for providing substratum by peristaltic pump for cultivating reactor; Described gas cupboard is for providing gas by taking out air exchange system for cultivating reactor; Described computer is for input control order; Described electrochemical workstation is for detecting in real time the data parameters of cultivating reactor according to the steering order of computer input.
2. the microorganism electrochemical system for cultured continuously, sampling and real-time detection according to claim 1, is characterized in that, described electrochemical workstation is also for inputting electricity irritation according to the steering order of computer input to cultivating reactor.
3. the microorganism electrochemical system for cultured continuously, sampling and real-time detection according to claim 1, is characterized in that, described computer is also for the detected data parameters of store electricity chem workstation.
4. according to claim 1 for cultured continuously, sampling and the real-time microorganism electrochemical system detecting, it is characterized in that, also comprise sampling system, wherein, described sampling system connects described cultivation reactor, and described sampling system is cultivated the culture of reactor for sampling.
5. according to claim 1 for cultured continuously, sampling and the real-time microorganism electrochemical system detecting, it is characterized in that, also comprise constant incubator, wherein, described cultivation reactor is arranged in described constant incubator, and described cultivation reactor is used to cultivation reactor to provide temperature adjustable environment.
6. according to claim 1 for cultured continuously, sampling and the real-time microorganism electrochemical system detecting, it is characterized in that, described cultivation reactor comprises exchange membrane, synthetic glass groove, silicone rubber O-ring, high pure graphite electrode, silicone rubber seal gasket, poly (methyl methacrylate) plate, screw, wherein, the both sides of described exchange membrane are symmetrical structure by screw respectively from inside to outside and fix successively organic glass guide channel, silicone rubber O-ring, high pure graphite electrode, silicone rubber seal gasket and poly (methyl methacrylate) plate.
7. the microorganism electrochemical system for cultured continuously, sampling and real-time detection according to claim 1, is characterized in that, described cultivation reactor comprises multipair parallelly cultivate reaction chamber.
8. the microorganism electrochemical system for cultured continuously, sampling and real-time detection according to claim 7, is characterized in that, described cultivation reaction chamber is for can independently carrying out the cultivation reaction chamber of cultured continuously.
CN201310467369.9A 2013-10-09 2013-10-09 Microbial electrochemical system for continuous culture, sampling and real-time detection Pending CN103571748A (en)

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Cited By (2)

* Cited by examiner, † Cited by third party
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CN104004655A (en) * 2013-10-09 2014-08-27 上海交通大学 Microbial electrochemical system for continuous culture, sampling and real-time detection
CN104255444A (en) * 2014-09-03 2015-01-07 上海农科种子种苗有限公司 Assistant selection system for green-keeping type corn seed selection

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CN107699483A (en) * 2017-10-30 2018-02-16 北华大学 A kind of medical test microbial cultivation equipment
CN107907698A (en) * 2017-12-14 2018-04-13 中国科学院深海科学与工程研究所 A kind of Biogeochemistry original position experimental provision for deep-sea
CN109026629B (en) * 2018-06-26 2019-12-27 杭州电子科技大学 Deep sea peristaltic pump and deep sea controllable long-term chemical reaction device
CN112834581B (en) * 2020-12-31 2023-10-03 陕西师范大学 Portable detection generator for dynamic detection, application and use method thereof

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KR100303611B1 (en) * 1999-07-07 2001-09-24 박호군 An Electrochemical Method for Enrichment of Microorganism, and a Biosensor for Analyzing Organic Substance and BOD
CN101413915B (en) * 2008-12-01 2012-05-16 中国科学院长春应用化学研究所 Method for rapidly detecting biochemical oxygen demand by using on site culture organism
CN102544562A (en) * 2012-02-27 2012-07-04 中国科学院城市环境研究所 Air-permeation cathode double-room microorganism fuel cell
CN103571748A (en) * 2013-10-09 2014-02-12 上海交通大学 Microbial electrochemical system for continuous culture, sampling and real-time detection

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104004655A (en) * 2013-10-09 2014-08-27 上海交通大学 Microbial electrochemical system for continuous culture, sampling and real-time detection
CN104255444A (en) * 2014-09-03 2015-01-07 上海农科种子种苗有限公司 Assistant selection system for green-keeping type corn seed selection

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Application publication date: 20140212