CN103549111B - The method for separating and preparing of protein in soy protein wastewater - Google Patents
The method for separating and preparing of protein in soy protein wastewater Download PDFInfo
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- 102000004169 proteins and genes Human genes 0.000 title claims abstract description 125
- 108010073771 Soybean Proteins Proteins 0.000 title claims abstract description 113
- 229940001941 soy protein Drugs 0.000 title claims abstract description 99
- 238000000034 method Methods 0.000 title claims abstract description 97
- 239000006260 foam Substances 0.000 claims abstract description 125
- 235000018102 proteins Nutrition 0.000 claims abstract description 124
- 239000007788 liquid Substances 0.000 claims abstract description 89
- 238000000926 separation method Methods 0.000 claims abstract description 36
- 235000010469 Glycine max Nutrition 0.000 claims abstract description 33
- 239000007787 solid Substances 0.000 claims abstract description 33
- 244000068988 Glycine max Species 0.000 claims abstract description 31
- 238000005507 spraying Methods 0.000 claims abstract description 25
- 238000004519 manufacturing process Methods 0.000 claims abstract description 21
- 239000002994 raw material Substances 0.000 claims abstract description 18
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- 235000013305 food Nutrition 0.000 claims abstract description 11
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- 238000001514 detection method Methods 0.000 claims abstract description 9
- 239000000047 product Substances 0.000 claims description 28
- 238000002306 biochemical method Methods 0.000 claims description 9
- 238000001556 precipitation Methods 0.000 claims description 8
- 239000006228 supernatant Substances 0.000 claims description 8
- ZTHYODDOHIVTJV-UHFFFAOYSA-N Propyl gallate Chemical compound CCCOC(=O)C1=CC(O)=C(O)C(O)=C1 ZTHYODDOHIVTJV-UHFFFAOYSA-N 0.000 claims description 6
- -1 cinnamic acid ester Chemical class 0.000 claims description 6
- BGNXCDMCOKJUMV-UHFFFAOYSA-N Tert-Butylhydroquinone Chemical compound CC(C)(C)C1=CC(O)=CC=C1O BGNXCDMCOKJUMV-UHFFFAOYSA-N 0.000 claims description 4
- CZBZUDVBLSSABA-UHFFFAOYSA-N butylated hydroxyanisole Chemical compound COC1=CC=C(O)C(C(C)(C)C)=C1.COC1=CC=C(O)C=C1C(C)(C)C CZBZUDVBLSSABA-UHFFFAOYSA-N 0.000 claims description 4
- 229960001755 resorcinol Drugs 0.000 claims description 4
- GEHJYWRUCIMESM-UHFFFAOYSA-L sodium sulfite Chemical compound [Na+].[Na+].[O-]S([O-])=O GEHJYWRUCIMESM-UHFFFAOYSA-L 0.000 claims description 4
- DWAQJAXMDSEUJJ-UHFFFAOYSA-M Sodium bisulfite Chemical compound [Na+].OS([O-])=O DWAQJAXMDSEUJJ-UHFFFAOYSA-M 0.000 claims description 3
- 238000005119 centrifugation Methods 0.000 claims description 3
- 235000010388 propyl gallate Nutrition 0.000 claims description 3
- 229940079827 sodium hydrogen sulfite Drugs 0.000 claims description 3
- 235000010267 sodium hydrogen sulphite Nutrition 0.000 claims description 3
- WBYWAXJHAXSJNI-VOTSOKGWSA-M .beta-Phenylacrylic acid Natural products [O-]C(=O)\C=C\C1=CC=CC=C1 WBYWAXJHAXSJNI-VOTSOKGWSA-M 0.000 claims description 2
- SPSPIUSUWPLVKD-UHFFFAOYSA-N 2,3-dibutyl-6-methylphenol Chemical compound CCCCC1=CC=C(C)C(O)=C1CCCC SPSPIUSUWPLVKD-UHFFFAOYSA-N 0.000 claims description 2
- ODJQKYXPKWQWNK-UHFFFAOYSA-N 3,3'-Thiobispropanoic acid Chemical compound OC(=O)CCSCCC(O)=O ODJQKYXPKWQWNK-UHFFFAOYSA-N 0.000 claims description 2
- QAQJMLQRFWZOBN-LAUBAEHRSA-N L-ascorbyl-6-palmitate Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@H](O)[C@H]1OC(=O)C(O)=C1O QAQJMLQRFWZOBN-LAUBAEHRSA-N 0.000 claims description 2
- 239000011786 L-ascorbyl-6-palmitate Substances 0.000 claims description 2
- 239000003490 Thiodipropionic acid Substances 0.000 claims description 2
- 235000010385 ascorbyl palmitate Nutrition 0.000 claims description 2
- 235000010354 butylated hydroxytoluene Nutrition 0.000 claims description 2
- 229930016911 cinnamic acid Natural products 0.000 claims description 2
- 235000013985 cinnamic acid Nutrition 0.000 claims description 2
- 238000010297 mechanical methods and process Methods 0.000 claims description 2
- WBYWAXJHAXSJNI-UHFFFAOYSA-N methyl p-hydroxycinnamate Natural products OC(=O)C=CC1=CC=CC=C1 WBYWAXJHAXSJNI-UHFFFAOYSA-N 0.000 claims description 2
- HRZFUMHJMZEROT-UHFFFAOYSA-L sodium disulfite Chemical compound [Na+].[Na+].[O-]S(=O)S([O-])(=O)=O HRZFUMHJMZEROT-UHFFFAOYSA-L 0.000 claims description 2
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- 238000002360 preparation method Methods 0.000 description 2
- 238000011084 recovery Methods 0.000 description 2
- 102000004407 Lactalbumin Human genes 0.000 description 1
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- 239000003513 alkali Substances 0.000 description 1
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- 238000005265 energy consumption Methods 0.000 description 1
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- 238000011017 operating method Methods 0.000 description 1
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Abstract
The present invention is the method for separating and preparing of protein in a kind of soy protein wastewater, and the method comprises: the collection of (1) soybean waste water and detection; (2) pretreatment of soy protein wastewater; (3) two-stage foam fraction factor technique; (4) Separation of Solid and Liquid and spraying dry.The method prevents the protein in separation process corrupt by adding antioxidant, determines the number range filling liquid height and these two parameters of operating time, finally obtains powdered proteins product.The present invention effectively reduces the protein concentration in soy protein wastewater, gained raffinate can be used as the raw material of soya oligose by membrane separation process, thus the cost of biochemical process processed soybeans proteinic wastewater and the production cost of soybean protein isolate is reduced, there is good economic benefit and social benefit, wherein protein quality percentage is up to 90%, can be used as food grade protein product.
Description
Technical field
Technical scheme of the present invention belongs to field of waste water treatment, is the method for separating and preparing of protein in soy protein wastewater specifically.
Background technology
Soy protein wastewater is producing with in alkali extraction-acid precipitation production soybean protein isolate process, its generation is 20 ~ 30 tons/soybean protein isolate per ton, and in this waste water containing being of high nutritive value in a large number, the protein of high-dissolvability, high foaming characteristic and high emulsibility.At present, soy protein wastewater is directly discharged into sewage treatment plant and utilizes common biochemical method to process by enterprise.This not only adds the production cost of soybean protein isolate, also cause a large amount of wastes of protein in waste water.So adopt suitable method reclaim and utilize the protein in soy protein wastewater can produce good Social and economic benef@.
At present, the main method reclaiming protein in soy protein wastewater comprises foamet, membrane separation process and ion-exchange etc.But, membrane separation process and the shortcoming that ion-exchange existence is invested and producing cost is high.Foamet gains great popularity because it has the advantages such as equipment is simple, small investment, energy consumption are low and pollution-free.Application foam fraction factor skill and technique reclaims albumen Quality Research in soy protein wastewater bibliographical information, the researchers such as Xie Jihong deliver on " East China University of Science's journal " the 23rd volume the 3rd phase in 1997 the 270 to 274 page and the 275 to 280 page " soy proteinaceous foam fraction factor research: I. operating procedure condition " and " soy proteinaceous foam fraction factor studies II. the Mathematical Modeling of foam fractionation process " two sections of papers to foamet recovery soy protein wastewater in protein conduct a preliminary study; Poplar has been delivered on " chemical industry progress " the 27th volume the 1st phase in 2008 the 92 to 97 page to equality researcher the foam fraction factor experiment of lactalbumin " in the soy protein wastewater " paper.Protein maximum concentration in the froth breaking liquid that they obtain is only 3.93g/L.The patent of invention ZL201010173810.9 of researcher's applications such as Wu Zhaoliang develops " in two-stage foam separation method soy protein concentrate waste water protein technique ".Protein concentration in the first order froth breaking liquid that they obtain is 22.5 ~ 32.5g/L.But find in experimentation, due to air oxidation, the froth breaking liquid obtained can send corrupt taste.To this this patent do not take any effective technology with ensure separation obtain the quality of protein.In addition, this patent does not determine the number range filling liquid height and these two important parameters of operating time yet.
Current research not yet carries out pretreatment to prevent the protein oxidation in foam fraction factor process to soy protein wastewater, and not yet obtains pulverous protein product.Therefore the method for separating and preparing of protein in invention soy protein wastewater, makes the problem of oxidation of protein in foam fraction factor process be solved, and obtains pulverous protein product.
At present, the technology Separation of Proteins preparation in soy protein wastewater being become powdered proteins product that in the soy protein wastewater of this patent, the method for separating and preparing of protein can reach has no bibliographical information.
Summary of the invention
The present invention is to reclaim and the protein in huge profit soy protein wastewater, and then reduce the cost of the production soybean protein isolate increase that biochemical process processed soybeans proteinic wastewater brings, the method for separating and preparing of protein in a kind of soy protein wastewater is proposed, the method prevents the protein in separation process corrupt by adding antioxidant, determine the number range filling liquid height and these two parameters of operating time, object embodies the efficiency of best separation, thus the Separation of Proteins preparation in soy protein wastewater is become pulverous protein product, truly realize the recovery of protein in soy protein wastewater.
Technical scheme of the present invention is:
A method for separating and preparing for protein in soy protein wastewater, comprises the following steps:
A. the collection of soybean waste water and detection:
Collect and produce soy protein wastewater, detect protein concentration, detect pH value;
B. the pretreatment of soy protein wastewater:
In the soy protein wastewater collected, add food grade antioxidants, add that quality is soy protein wastewater quality 0.015 ~ 0.050%, then regulate soy protein wastewater temperature to 50 ~ 65 DEG C;
C. two-stage foam fraction factor technique:
First order foam fraction factor technique: foam fraction factor is carried out to pretreated soybean proteinic wastewater, be separated by bubble tower interval, temperature is 50 ~ 65 DEG C, superficial gas velocity is 0.93 ~ 1.85mm/s, height of liquid layer is 0.7 ~ 1.4m, foam layer height is 0.6 ~ 1.0m, the one-period operating time was at 6.0 ~ 14.0 hours, and the froth breaking liquid of the first order is obtained through debubbling method, its volume is 10 ~ 25% of soy protein wastewater volume, protein concentration is 6.0 ~ 16.0g/L, this froth breaking liquid proceeds to steps d and directly carries out Separation of Solid and Liquid and spraying dry as the raw material producing soybean protein, raffinate remaining in foam separating tower, its protein concentration is 0.6 ~ 1.5g/L, as the charging of second level foam fraction factor,
Second level foam fraction factor technique: foam fraction factor is carried out to raffinate remaining in first order foam separating tower, be separated by bubble tower interval, temperature is 20 ~ 40 DEG C, superficial gas velocity is 1.54 ~ 3.40mm/s, height of liquid layer is 0.8 ~ 1.6m, foam layer height is 0.6 ~ 1.0m, the one-period operating time was at 3.0 ~ 9.0 hours, and the froth breaking liquid of the second level is obtained through debubbling method, its volume is 20 ~ 35% of soy protein wastewater volume, its protein concentration is 1.6 ~ 4.2g/L, this froth breaking liquid mixes with not pretreated soy protein wastewater, and after pretreatment as the charging of the first order foam fraction factor technique in next production cycle, raffinate remaining in foam separating tower, its protein concentration is 0.2 ~ 0.9g/L, as the raw material of soya oligose by membrane separation process or discharge after general biochemical method process,
D. Separation of Solid and Liquid and spraying dry:
The froth breaking liquid obtained by first order foam fraction factor carries out natural subsidence process, the one-period operating time was at 2.0 ~ 4.0 hours, obtain turbid solution, its percent solids content is 4 ~ 8%, this turbid solution is carried out solid-liquid centrifugation, in supernatant, protein concentration is 1.3 ~ 4.3g/L, mixes with not pretreated soy protein wastewater, and after pretreatment as the charging of the first order foam fraction factor technique in next production cycle; In precipitation, percent solids content is 10 ~ 20%, after carrying out spraying dry, obtains powdered proteins product, and wherein the mass percent of protein is 70 ~ 90%, and the yield of protein is 35 ~ 70%.
Protein concentration in soy protein wastewater in described step a is 1.5 ~ 4.0g/L, and pH value is 4.3 ~ 4.5.
Described food grade antioxidants is sodium hydrogensulfite, sodium metasulfite, sodium sulfite, butylhydroxy anisole, dibutyl hydroxy toluene, TBHQ, n-propyl gallate, ascorbyl palmitate, thiodipropionic acid dilauryl cinnamic acid ester or 4-hexyl resorcin.
Described debubbling method is Mechanical Method or hot froth breaking method above.
Beneficial effect of the present invention is:
1., by adding a small amount of antioxidant in soybean waste water, ensure the quality being separated the protein product obtained;
2. determine suitable dress liquid height and operating time, realize the high efficiente callback of protein, the highest yield is 70%, and this technique suitability for industrialized is produced;
3. obtain powdered proteins product, wherein protein quality percentage is up to 90%, can be used as food grade protein product;
4. secondary foam fraction factor technique effectively reduces the protein concentration in soy protein wastewater, gained raffinate can be used as the raw material of soya oligose by membrane separation process, thus reduce the cost of biochemical process processed soybeans proteinic wastewater and the production cost of soybean protein isolate, there is good economic benefit and social benefit.
Detailed description of the invention
Embodiment 1
A. the collection of soybean waste water and detection:
Collect and produce soy protein wastewater, with GB/T20371-2006, to detect this waste water protein concentration be 1.5g/L, pH is 4.4;
B. the pretreatment of soy protein wastewater:
In the soy protein wastewater collected, add food-grade sodium hydrogensulfite, the quality added is 0.015% of soy protein wastewater quality, then regulates soy protein wastewater temperature to 50 ~ 55 DEG C;
C. two-stage foam fraction factor technique:
First order foam fraction factor technique: foam fraction factor is carried out to pretreated soybean proteinic wastewater, be separated by bubble tower interval, temperature is 50 ~ 55 DEG C, superficial gas velocity is 1.54 ~ 1.85mm/s, height of liquid layer is 1.3 ~ 1.4m, foam layer height is 0.6 ~ 0.8m, the one-period operating time was at 6.0 ~ 8.0 hours, and the froth breaking liquid of the first order is obtained through mechanical defoaming, its volume is 10 ~ 15% of soy protein wastewater volume, protein concentration is 6.0 ~ 8.0g/L, this froth breaking liquid proceeds to steps d and directly carries out Separation of Solid and Liquid and spraying dry as the raw material producing soybean protein, raffinate remaining in foam separating tower, its protein concentration is 0.6 ~ 0.7g/L, as the charging of second level foam fraction factor,
Second level foam fraction factor technique: foam fraction factor is carried out to raffinate remaining in first order foam separating tower, be separated by bubble tower interval, temperature is 20 ~ 25 DEG C, superficial gas velocity is 3.09 ~ 3.40mm/s, height of liquid layer is 1.4 ~ 1.6m, foam layer height is 0.6m, the one-period operating time was at 3.0 ~ 4.0 hours, and the froth breaking liquid of the second level is obtained through mechanical defoaming, its volume is 20 ~ 25% of soy protein wastewater volume, protein concentration is 1.6 ~ 1.8g/L, this froth breaking liquid mixes with not pretreated soy protein wastewater, and after pretreatment as the charging of the first order foam fraction factor technique in next production cycle, the protein concentration of raffinate remaining in foam separating tower is 0.2 ~ 0.3g/L, and this raffinate is as the raw material of soya oligose by membrane separation process or discharge after general biochemical method process,
D. Separation of Solid and Liquid and spraying dry:
The froth breaking liquid obtained by first order foam fraction factor carries out natural subsidence process, the one-period operating time was at 3.5 ~ 4.0 hours, obtain turbid solution, its percent solids content is 4 ~ 5%, this turbid solution is carried out solid-liquid centrifugation, in supernatant, protein concentration is 1.3 ~ 1.7g/L, mixes with not pretreated soy protein wastewater, and after pretreatment as the charging of the first order foam fraction factor technique in next production cycle; In precipitation, percent solids content is 10 ~ 13%, after carrying out spraying dry, obtain the protein product of cream powder, detect according to GB/T20371-2006 examination criteria, the protein quality percentage of product is 70 ~ 75%, reaches the standard of food grade protein product; The yield of protein is 35 ~ 40%.
Embodiment 2
A. the collection of soybean waste water and detection:
Collect and produce soy protein wastewater, with GB/T20371-2006, to detect this waste water protein concentration be 2.0g/L, pH is 4.3;
B. the pretreatment of soy protein wastewater:
In the soy protein wastewater collected, add food-grade butylhydroxy anisole, the quality added is 0.030% of soy protein wastewater quality, then regulates soy protein wastewater temperature to 55 ~ 60 DEG C;
C. two-stage foam fraction factor technique:
First order foam fraction factor technique: foam fraction factor is carried out to pretreated soybean proteinic wastewater, be separated by bubble tower interval, temperature is 55 ~ 60 DEG C, superficial gas velocity is 1.45 ~ 1.54mm/s, height of liquid layer is 1.1 ~ 1.2m, foam layer height is 0.6 ~ 0.8m, the one-period operating time was at 8.0 ~ 10.0 hours, and the froth breaking liquid of the first order is obtained through mechanical defoaming, its volume is 15 ~ 18% of soy protein wastewater volume, protein concentration is 8.0 ~ 10.0g/L, this froth breaking liquid proceeds to steps d and directly carries out Separation of Solid and Liquid and spraying dry as the raw material producing soybean protein, raffinate remaining in foam separating tower, its protein concentration is 0.8 ~ 1.0g/L, as the charging of second level foam fraction factor,
Second level foam fraction factor technique: foam fraction factor is carried out to raffinate remaining in first order foam separating tower, be separated by bubble tower interval, temperature is 25 ~ 30 DEG C, superficial gas velocity is 2.46 ~ 2.56mm/s, height of liquid layer is 1.2 ~ 1.4m, foam layer height is 0.6 ~ 0.8m, the one-period operating time was at 5.0 ~ 6.0 hours, and the froth breaking liquid of the second level is obtained through mechanical defoaming method, its volume is 25 ~ 30% of soy protein wastewater volume, protein concentration is 2.1 ~ 2.3g/L, this froth breaking liquid mixes with not pretreated soy protein wastewater, and after pretreatment as the charging of the first order foam fraction factor technique in next production cycle, the protein concentration of raffinate remaining in foam separating tower is 0.5 ~ 0.6g/L, and this raffinate is as the raw material of soya oligose by membrane separation process or discharge after general biochemical method process,
D. Separation of Solid and Liquid and spraying dry:
The froth breaking liquid obtained by first order foam fraction factor carries out natural subsidence process, the one-period operating time was at 3.0 ~ 3.5 hours, obtain turbid solution, its percent solids content is 5 ~ 6%, this turbid solution is carried out centrifugal, in supernatant, protein concentration is 1.8 ~ 2.3g/L, mixes with not pretreated soy protein wastewater, and after pretreatment as the charging of the first order foam fraction factor technique in next production cycle; Percent solids content in precipitation is 13 ~ 15%, after carrying out spraying dry, obtain the protein product of cream powder, detect according to GB/T20371-2006 examination criteria, the protein quality percentage of product is 75 ~ 80%, reaches the standard of food grade protein product.The yield of protein is 45 ~ 50%.
Embodiment 3
A. the collection of soybean waste water and detection:
Collect and produce soy protein wastewater, with GB/T20371-2006, to detect this waste water protein concentration be 3.0g/L, pH is 4.3;
B. the pretreatment of soy protein wastewater:
In the soy protein wastewater collected, add food-grade n-propyl gallate, the quality added is 0.040% of soy protein wastewater quality, then regulates soy protein wastewater temperature to 60 ~ 65 DEG C;
C. two-stage foam fraction factor technique:
First order foam fraction factor technique: foam fraction factor is carried out to pretreated soybean proteinic wastewater, be separated by bubble tower interval, temperature is 60 ~ 65 DEG C, superficial gas velocity is 1.20 ~ 1.23mm/s, height of liquid layer is 0.9 ~ 1.0m, foam layer height is 0.8 ~ 0.9m, the one-period operating time was at 10.0 ~ 12.0 hours, and the froth breaking liquid of the first order is obtained through mechanical defoaming, its volume is 20 ~ 25% of soy protein wastewater volume, protein concentration is 9.0 ~ 10.0g/L, this froth breaking liquid proceeds to steps d and directly carries out Separation of Solid and Liquid and spraying dry as the raw material producing soybean protein, raffinate remaining in foam separating tower, its protein concentration is 1.2 ~ 1.4g/L, as the charging of second level foam fraction factor,
Second level foam fraction factor technique: foam fraction factor is carried out to raffinate remaining in first order foam separating tower, be separated by bubble tower interval, temperature is 30 ~ 35 DEG C, superficial gas velocity is 2.10 ~ 2.16mm/s, height of liquid layer is 1.0 ~ 1.2m, foam layer height is 0.8 ~ 0.9m, the one-period operating time was at 6.0 ~ 7.0 hours, and the froth breaking liquid of the second level is obtained through mechanical defoaming method, its volume is 30 ~ 35% of soy protein wastewater volume, protein concentration is 3.1 ~ 3.2g/L, this froth breaking liquid mixes with not pretreated soy protein wastewater, and after pretreatment as the charging of the first order foam fraction factor technique in next production cycle, the protein concentration of raffinate remaining in foam separating tower is 0.7 ~ 0.9g/L, and this raffinate is as the raw material of soya oligose by membrane separation process or discharge after general biochemical method process,
D. Separation of Solid and Liquid and spraying dry:
The froth breaking liquid obtained by first order foam fraction factor carries out natural subsidence process, the one-period operating time was at 2.0 ~ 2.5 hours, obtain turbid solution, its percent solids content is 6 ~ 7%, this turbid solution is carried out centrifugal, in supernatant, protein concentration is 2.7 ~ 3.3g/L, mixes with not pretreated soy protein wastewater, and after pretreatment as the charging of the first order foam fraction factor technique in next production cycle; Percent solids content in precipitation is 16 ~ 18%, after carrying out spraying dry, obtain the protein product of cream powder, detect according to GB/T20371-2006 examination criteria, the protein quality percentage of product is 80 ~ 85%, reaches the standard of food grade protein product.The yield of protein is 60 ~ 65%.
Embodiment 4
A. the collection of soybean waste water and detection:
Collect and produce soy protein wastewater, with GB/T20371-2006, to detect this waste water protein concentration be 4.0g/L, pH is 4.5;
B. the pretreatment of soy protein wastewater:
In the soy protein wastewater collected, add food-grade 4-hexyl resorcin, the quality added is 0.050% of soy protein wastewater quality, then regulates soy protein wastewater temperature to 60 ~ 65 DEG C;
C. two-stage foam fraction factor technique:
First order foam fraction factor technique: foam fraction factor is carried out to pretreated soybean proteinic wastewater, be separated by bubble tower interval, temperature is 60 ~ 65 DEG C, superficial gas velocity is 0.93 ~ 1.10mm/s, height of liquid layer is 0.7 ~ 0.8m, foam layer height is 0.9 ~ 1.0m, the one-period operating time was at 12.0 ~ 14.0 hours, and the froth breaking liquid of the first order is obtained through mechanical defoaming, its volume is 15 ~ 20% of soy protein wastewater volume, protein concentration is 14.0 ~ 16.0g/L, this froth breaking liquid proceeds to steps d and directly carries out Separation of Solid and Liquid and spraying dry as the raw material producing soybean protein, raffinate remaining in foam separating tower, its protein concentration is 1.4 ~ 1.5g/L, as the charging of second level foam fraction factor,
Second level foam fraction factor technique: foam fraction factor is carried out to raffinate remaining in first order foam separating tower, be separated by bubble tower interval, temperature is 35 ~ 40 DEG C, superficial gas velocity is 1.54 ~ 1.63mm/s, height of liquid layer is 0.8 ~ 0.9m, foam layer height is 0.9 ~ 1.0m, the one-period operating time was at 8.0 ~ 9.0 hours, and the froth breaking liquid of the second level is obtained through mechanical defoaming method, its volume is 20 ~ 25% of soy protein wastewater volume, protein concentration is 4.0 ~ 4.2g/L, this froth breaking liquid mixes with not pretreated soy protein wastewater, and after pretreatment as the charging of the first order foam fraction factor technique in next production cycle, the protein concentration of raffinate remaining in foam separating tower is 0.6 ~ 0.8g/L, and this raffinate is as the raw material of soya oligose by membrane separation process or discharge after general biochemical method process,
D. Separation of Solid and Liquid and spraying dry:
The froth breaking liquid obtained by first order foam fraction factor carries out natural subsidence process, the one-period operating time was at 2.0 ~ 2.5 hours, obtain turbid solution, its percent solids content is 7 ~ 8%, this turbid solution is carried out centrifugal, in supernatant, protein concentration is 3.8 ~ 4.3g/L, mixes with not pretreated soy protein wastewater, and after pretreatment as the charging of the first order foam fraction factor technique in next production cycle; Percent solids content in precipitation is 19 ~ 20%, after carrying out spraying dry, obtain the protein product of cream powder, detect according to GB/T20371-2006 examination criteria, the protein quality percentage of product is 85 ~ 90%, reaches the standard of food grade protein product.The yield of protein is 65 ~ 70%.
Comparative example 1
A. the collection of soybean waste water and detection:
Collect and produce soy protein wastewater, with GB/T20371-2006, to detect this waste water protein concentration be 2.0g/L, pH is 4.3;
B. the pretreatment of soy protein wastewater:
In the soy protein wastewater collected, add food-grade butylhydroxy anisole, the quality added is 0.030% of soy protein wastewater quality, then regulates soy protein wastewater temperature to 55 ~ 60 DEG C;
C. two-stage foam fraction factor technique:
First order foam fraction factor technique: foam fraction factor is carried out to pretreated soybean proteinic wastewater, be separated by bubble tower interval, temperature is 55 ~ 60 DEG C, superficial gas velocity is 1.45 ~ 1.54mm/s, height of liquid layer is 0.5 ~ 0.6m, foam layer height is 0.6 ~ 0.8m, the one-period operating time was at 4.0 ~ 5.0 hours, and the froth breaking liquid of the first order is obtained through mechanical defoaming, its volume is 3 ~ 5% of soy protein wastewater volume, protein concentration is 6.0 ~ 8.0g/L, and this froth breaking liquid proceeds to steps d and directly carries out Separation of Solid and Liquid and spraying dry as the raw material producing soybean protein; Raffinate remaining in foam separating tower, its protein concentration is 1.5 ~ 1.7g/L, as the charging of second level foam fraction factor;
Second level foam fraction factor technique: foam fraction factor is carried out to raffinate remaining in first order foam separating tower, be separated by bubble tower interval, temperature is 25 ~ 30 DEG C, superficial gas velocity is 2.46 ~ 2.56mm/s, height of liquid layer is 0.6 ~ 0.7m, foam layer height is 0.6 ~ 0.8m, the one-period operating time was at 2.0 ~ 3.0 hours, and the froth breaking liquid of the second level is obtained through mechanical defoaming method, its volume is 5 ~ 7% of soy protein wastewater volume, protein concentration is 2.1 ~ 2.2g/L, this froth breaking liquid mixes with not pretreated soy protein wastewater, and after pretreatment as the charging of the first order foam fraction factor technique in next production cycle, the protein concentration of raffinate remaining in foam separating tower is 1.3 ~ 1.5g/L, and this raffinate is as the raw material of soya oligose by membrane separation process or discharge after general biochemical method process,
D. Separation of Solid and Liquid and spraying dry:
The froth breaking liquid obtained by first order foam fraction factor carries out natural subsidence process, the one-period operating time was at 3.0 ~ 3.5 hours, obtain turbid solution, its percent solids content is 3 ~ 4%, this turbid solution is carried out centrifugal, in supernatant, protein concentration is 1.8 ~ 2.1g/L, mixes with not pretreated soy protein wastewater, and after pretreatment as the charging of the first order foam fraction factor technique in next production cycle; Percent solids content in precipitation is 13 ~ 15%, after carrying out spraying dry, obtain the protein product of cream powder, detect according to GB/T20371-2006 examination criteria, the protein quality percentage of product is 75 ~ 80%, reaches the standard of food grade protein product.The yield of protein is 5 ~ 10%.
Contrast with embodiment 2, when the dress liquid height of two-stage foam fraction factor technique and the parameter value of operating time are lower than range of parameter values of the present invention, the yield of protein drops to less than 10%.This is because the reduction of dress liquid height shortens the time of staying of bubble in liquid, the density of protein on bubble surface is caused to reduce; The shortening of operating time decreases the total amount of the protein be adsorbed on bubble surface.Final Protein yield is comparatively low to moderate less than 10%.
Comparative example 2
A. the collection of soybean waste water and detection:
Collect and produce soy protein wastewater, with GB/T20371-2006, to detect this waste water protein concentration be 4.0g/L, pH is 4.5;
B. the pretreatment of soy protein wastewater:
In the soy protein wastewater collected, add food-grade 4-hexyl resorcin, the quality added is 0.050% of soy protein wastewater quality, then regulates soy protein wastewater temperature to 60 ~ 65 DEG C;
C. two-stage foam fraction factor technique:
First order foam fraction factor technique: foam fraction factor is carried out to pretreated soybean proteinic wastewater, be separated by bubble tower interval, temperature is 60 ~ 65 DEG C, superficial gas velocity is 0.93 ~ 1.10mm/s, height of liquid layer is 1.5 ~ 1.6m, foam layer height is 0.9 ~ 1.0m, the one-period operating time was at 22.0 ~ 25.0 hours, and the froth breaking liquid of the first order is obtained through mechanical defoaming, its volume is 20 ~ 25% of soy protein wastewater volume, protein concentration is 13.0 ~ 15.0g/L, this froth breaking liquid proceeds to steps d and directly carries out Separation of Solid and Liquid and spraying dry as the raw material producing soybean protein, raffinate remaining in foam separating tower, its protein concentration is 1.3 ~ 1.5g/L, as the charging of second level foam fraction factor,
Second level foam fraction factor technique: foam fraction factor is carried out to raffinate remaining in first order foam separating tower, be separated by bubble tower interval, temperature is 35 ~ 40 DEG C, superficial gas velocity is 1.54 ~ 1.63mm/s, height of liquid layer is 1.6 ~ 1.7m, foam layer height is 0.9 ~ 1.0m, the one-period operating time was at 15.0 ~ 17.0 hours, and the froth breaking liquid of the second level is obtained through mechanical defoaming method, its volume is 22 ~ 27% of soy protein wastewater volume, protein concentration is 4.0 ~ 4.2g/L, this froth breaking liquid mixes with not pretreated soy protein wastewater, and after pretreatment as the charging of the first order foam fraction factor technique in next production cycle, the protein concentration of raffinate remaining in foam separating tower is 0.5 ~ 0.7g/L, and this raffinate is as the raw material of soya oligose by membrane separation process or discharge after general biochemical method process,
D. Separation of Solid and Liquid and spraying dry:
The froth breaking liquid obtained by first order foam fraction factor carries out natural subsidence process, the one-period operating time was at 2.0 ~ 2.5 hours, obtain turbid solution, its percent solids content is 7 ~ 8%, this turbid solution is carried out centrifugal, in supernatant, protein concentration is 3.8 ~ 4.1g/L, mixes with not pretreated soy protein wastewater, and after pretreatment as the charging of the first order foam fraction factor technique in next production cycle; Percent solids content in precipitation is 19 ~ 20%, and after carrying out spraying dry, obtain cream powder and have the protein product of rotten to the corn taste, detect according to GB/T20371-2006 examination criteria, the protein quality percentage of product is 85 ~ 90%.The yield of protein is 65 ~ 70%.
Contrast with embodiment 4, when the dress liquid height parameter of two-stage foam fraction factor technique is higher than range of parameter values of the present invention, the operating time will double to extend and just can reach Protein yield in the same manner as in Example 4.But cross long bubbling, make added antioxidant be consumed totally, the froth breaking liquid of one-level foam fraction factor technique sends corrupt taste, has had a strong impact on the quality of the protein product obtained.Therefore, the antioxidant that the present invention specifies addition, dress liquid height and the range of parameter values of operating time match, with the high-quality of the high efficiency and protein product that ensure two-stage foam fraction factor technique.
Claims (1)
1. the method for separating and preparing of protein in soy protein wastewater, is characterized by and comprise the following steps:
A. the collection of soybean waste water and detection:
Collect and produce soy protein wastewater, detect protein concentration, detect pH value;
B. the pretreatment of soy protein wastewater:
In the soy protein wastewater collected, add food grade antioxidants, add that quality is soy protein wastewater quality 0.015 ~ 0.050%, then regulate soy protein wastewater temperature to 50 ~ 65 DEG C;
C. two-stage foam fraction factor technique:
First order foam fraction factor technique: foam fraction factor is carried out to pretreated soybean proteinic wastewater, be separated by bubble tower interval, temperature is 50 ~ 65 DEG C, superficial gas velocity is 0.93 ~ 1.85mm/s, height of liquid layer is 0.7 ~ 1.4m, foam layer height is 0.6 ~ 1.0m, the one-period operating time was at 6.0 ~ 14.0 hours, and the froth breaking liquid of the first order is obtained through debubbling method, its volume is 10 ~ 25% of soy protein wastewater volume, protein concentration is 6.0 ~ 16.0g/L, this froth breaking liquid proceeds to steps d and directly carries out Separation of Solid and Liquid and spraying dry as the raw material producing soybean protein, raffinate remaining in foam separating tower, its protein concentration is 0.6 ~ 1.5g/L, as the charging of second level foam fraction factor,
Second level foam fraction factor technique: foam fraction factor is carried out to raffinate remaining in first order foam separating tower, be separated by bubble tower interval, temperature is 20 ~ 40 DEG C, superficial gas velocity is 1.54 ~ 3.40mm/s, height of liquid layer is 0.8 ~ 1.6m, foam layer height is 0.6 ~ 1.0m, the one-period operating time was at 3.0 ~ 9.0 hours, and the froth breaking liquid of the second level is obtained through debubbling method, its volume is 20 ~ 35% of soy protein wastewater volume, its protein concentration is 1.6 ~ 4.2g/L, this froth breaking liquid mixes with not pretreated soy protein wastewater, and after pretreatment as the charging of the first order foam fraction factor technique in next production cycle, raffinate remaining in foam separating tower, its protein concentration is 0.2 ~ 0.9g/L, as the raw material of soya oligose by membrane separation process or discharge after general biochemical method process,
D. Separation of Solid and Liquid and spraying dry:
The froth breaking liquid obtained by first order foam fraction factor carries out natural subsidence process, the one-period operating time was at 2.0 ~ 4.0 hours, obtain turbid solution, its percent solids content is 4 ~ 8%, this turbid solution is carried out solid-liquid centrifugation, in supernatant, protein concentration is 1.3 ~ 4.3g/L, mixes with not pretreated soy protein wastewater, and after pretreatment as the charging of the first order foam fraction factor technique in next production cycle; In precipitation, percent solids content is 10 ~ 20%, after carrying out spraying dry, obtains powdered proteins product, and wherein the mass percent of protein is 70 ~ 90%, and the yield of protein is 35 ~ 70%;
Protein concentration in soy protein wastewater in described step a is 1.5 ~ 4.0g/L, and pH value is 4.3 ~ 4.5;
Described food grade antioxidants is sodium hydrogensulfite, sodium metasulfite, sodium sulfite, butylhydroxy anisole, dibutyl hydroxy toluene, TBHQ, n-propyl gallate, ascorbyl palmitate, thiodipropionic acid dilauryl cinnamic acid ester or 4-hexyl resorcin;
Described debubbling method is Mechanical Method or hot froth breaking method.
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