CN103543276A - Kit for combined detection of HCG (human chorionic gonadotropin) and urinary system infection - Google Patents
Kit for combined detection of HCG (human chorionic gonadotropin) and urinary system infection Download PDFInfo
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- CN103543276A CN103543276A CN201310538904.5A CN201310538904A CN103543276A CN 103543276 A CN103543276 A CN 103543276A CN 201310538904 A CN201310538904 A CN 201310538904A CN 103543276 A CN103543276 A CN 103543276A
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- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54366—Apparatus specially adapted for solid-phase testing
- G01N33/54386—Analytical elements
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- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/74—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving hormones or other non-cytokine intercellular protein regulatory factors such as growth factors, including receptors to hormones and growth factors
- G01N33/76—Human chorionic gonadotropin including luteinising hormone, follicle stimulating hormone, thyroid stimulating hormone or their receptors
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- G—PHYSICS
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- G01N2800/00—Detection or diagnosis of diseases
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Abstract
The invention relates to a kit for the combined detection of HCG (human chorionic gonadotropin) and urinary system infection, and belongs to the technical field of detection. The kit comprises an HCG immunochromatography test strip and one or more of urine protein test paper, leucocyte test paper, feces occult blood test paper and nitrite test paper. Preferably, a conjugate pad and a chromatography membrane of the HCG immunochromatography test strip are wrapped by a corresponding immunolabelling antibody and a corresponding detection antibody respectively; the HCG immunochromatography test strip is arranged in parallel with one or more of the urine protein test paper, the leucocyte test paper, the feces occult blood test paper and the nitrite test paper or is arranged on the upper and lower surfaces of a bottom plate. The kit can be used for simultaneously detecting the HCG and urinary system infection, and is simple, convenient and suitable for large-scale popularization and application.
Description
Technical field
The present invention relates to a kind of human chorionic gonadotrophin and urinary system infection contamination joint inspection kit, particularly a kind of human chorionic gonadotrophin (HCG) and urinary system infection contamination joint inspection kit, belong to detection technique field.
Background technology
Human chorionic gonadotrophin (HCG), it is a kind of glycoprotein hormones with promotion gonad development by the secretion of tire basin syneytiotrophoblast, be present in pregnant woman's blood, urine, colostrum, amniotic fluid and fetus body, in First Trimester HCG secretory volume, increase and be exceedingly fast, during to pregnant 8 ~ 10 weeks, serum-concentration peaks, at the gestation initial stage, rule HCG ratio in serum is the highest, its content is substantially parallel in blood, urine, being determined at clinically of HCG correlation molecule is widely used, and comprising: for the diagnosis of early pregnancy; The appearance (as threatened abortion, Down syndrome etc.) of prompting abnormal pregnancy; Diagnose and monitor pregnant trophoblastic disease (vesicular mole, suede cancer etc.) etc.
Urinary system infection contamination is clinical common infectious diseases. women's incidence of disease is apparently higher than the male sex, and urinary system infection contamination very easily appears in pregnancy duration.
Widespread use colloidal gold method carries out fast detecting to HCG clinically, and urinary system infection contamination detects also multiple detection method, comprises urinalysis, bacteriology checking etc., but not yet find the product of HCG and urinary system infection contamination joint inspection.As HCG and urinary system infection contamination are carried out to joint inspection, by oneself, sample, once can detect simultaneously, simple and convenient.
Summary of the invention
The object of the invention is to overcome above-mentioned weak point, by primary sample, HCG and urinary system infection contamination are detected, a kind of human chorionic gonadotrophin HCG and urinary system infection contamination joint inspection kit are provided, apply easyly, be suitable for large-scale promotion application.
According to technical scheme provided by the invention, and a urinary system infection contamination joint inspection kit, comprise the first base plate, in the one side of the first base plate, be provided with chromatographic film, the front end of chromatographic film arranges opaque diaphragm, and the front end of opaque diaphragm arranges arrow; The front end of arrow arranges pad; In chromatographic film, be also disposed with detection line and nature controlling line, chromatographic film rear end is provided with adsorptive pads.
Also comprise infection index detection zone.
Described infection index detection zone is attached on the second base plate or is compound on any one surface of the first base plate.
In the one side of the first base plate, be provided with chromatographic film, the front end of chromatographic film arranges pad, and the place ahead of pad also arranges sample pad; In chromatographic film, be also disposed with detection line and nature controlling line, chromatographic film rear end is provided with adsorptive pads; On the lower surface of the first base plate, be also provided with infection index detection zone.
Described infection index detection zone is provided with urine Protein Detection test paper, leucocyte Test paper, one or more in fecal occult blood Test paper and nitrite Test paper.
Described urine Protein Detection test paper, leucocyte Test paper, the first base plate of fecal occult blood Test paper and nitrite Test paper or the material of the second base plate are a kind of in PET, PVC, PE, PP or PS, long is 10-300mm, wide 2-30mm, thickness is 0.1-1.5mm.
Described urine Protein Detection test paper, leucocyte Test paper, the basic material of fecal occult blood Test paper and nitrite Test paper is cellulose filter paper, glass fiber filter paper, chromatographic paper, polyester film or nylon membrane; Described test paper be shaped as square, rectangle, circle, ellipse, triangle, rhombus or trapezoidal; Size is long 1-30 mm; Wide 1-30 mm; Thickness 0.01-5 mm; Spacing between multiple test paper is 1-30 mm.
On described pad, be coated with human chorionic gonadotrophin HCG and detect antibody.
In described chromatographic film 6, be coated with HCG and detect antibody.
On the pad of described HCG immunochromatographydetecting detecting test strip, there is the immune labeled antibody of HCG, in the chromatographic film of described HCG immunochromatographydetecting detecting test strip, be coated with HCG and detect antibody; Described urine Protein Detection test paper, leucocyte Test paper, fecal occult blood Test paper and nitrite Test paper are by urine protein assay reagent, leucocyte detects reagent, and fecal occult blood detects reagent and nitrite detection reagent is solidificated in cellulose filter paper, glass fiber filter paper, chromatographic paper, polyester film or nylon membrane, the covering of employing level or built-in embedding grammar are placed in the upper and lower surface of base plate, and base plate consists of transparent or opaque plastics material.
Beneficial effect of the present invention: the present invention can sample by oneself, primary sample can detect HCG and urinary system infection contamination simultaneously, simple and convenient, is applicable to large-scale promotion application.
Accompanying drawing explanation
Fig. 1 is embodiment 1 first base plate 10 structural representations.
Fig. 2 is embodiment 1 second base plate 11 structural representations.
Fig. 3 is embodiment 2 structural representations.
Fig. 4 is embodiment 3 structural representations.
Fig. 5 is embodiment 4 structural representations.
Fig. 6 is embodiment 1 infection index detection zone structural representation.
Fig. 7 is embodiment 2 infection index detection zone structural representations.
Fig. 8 is embodiment 3 infection index detection zone structural representations.
Fig. 9 is embodiment 4 infection index detection zone structural representations.
Embodiment
As shown in Fig. 1,2,5, and a urinary system infection contamination joint inspection kit, comprise the first base plate 10, in the one side of the first base plate 10, be provided with chromatographic film 6, the front end of chromatographic film 6 arranges opaque diaphragm 3, and the front end of opaque diaphragm 3 arranges arrow 2; The front end of arrow 2 arranges pad 1; In chromatographic film 6, be also disposed with detection line 4 and nature controlling line 5, chromatographic film 6 rear ends are provided with adsorptive pads 7.
Also comprise infection index detection zone 8.Described infection index detection zone 8 is attached to the upper surface of the first base plate 10.
Described infection index detection zone 8 is provided with urine Protein Detection test paper.Described HCG immunochromatographydetecting detecting test strip, and urinary system infection contamination index detection zone, comprise urine Protein Detection test paper, is placed in the upper surface of base plate 10 with horizontal coverage mode or built-in embedded mode.
As shown in Fig. 3,7, and a urinary system infection contamination joint inspection kit, comprise the first base plate 10, in the one side of the first base plate 10, be provided with chromatographic film 6, the front end of chromatographic film 6 arranges opaque diaphragm 3, and the front end of opaque diaphragm 3 arranges arrow 2; The front end of arrow 2 arranges pad 1; In chromatographic film 6, be also disposed with detection line 4 and nature controlling line 5, chromatographic film 6 rear ends are provided with adsorptive pads 7.
Also comprise infection index detection zone 8.Described infection index detection zone 8 is attached on the second base plate 11.
Described infection index detection zone 8 is provided with leucocyte Test paper, fecal occult blood Test paper.With horizontal coverage mode or built-in embedded mode, be placed in the upper surface of base plate, and be set up in parallel on draw-in groove with HCG immunochromatographydetecting detecting test strip.
As shown in Fig. 4,8, and a urinary system infection contamination joint inspection kit, comprise the first base plate 10, in the one side of the first base plate 10, be provided with chromatographic film 6, the front end of chromatographic film 6 arranges pad 1, and the place ahead of pad 1 also arranges sample pad 9; In chromatographic film 6, be also disposed with detection line 4 and nature controlling line 5, chromatographic film 6 rear ends are provided with adsorptive pads 7; On the lower surface of the first base plate 10, be also provided with infection index detection zone 8.
Described infection index detection zone 8 is attached on the lower surface of the first base plate 10.
Described infection index detection zone 8 is provided with leucocyte Test paper, fecal occult blood Test paper and nitrite Test paper, with horizontal coverage mode or built-in embedded mode, be placed in the surface of base plate, and be placed in respectively the upper and lower surface of base plate with HCG immunity chromatography detection test paper.
As shown in Fig. 5,9, and a urinary system infection contamination joint inspection kit, comprise the first base plate 10, in the one side of the first base plate 10, be provided with chromatographic film 6, the front end of chromatographic film 6 arranges opaque diaphragm 3, and the front end of opaque diaphragm 3 arranges arrow 2; The front end of arrow 2 arranges pad 1; In chromatographic film 6, be also disposed with detection line 4 and nature controlling line 5, chromatographic film 6 rear ends are provided with adsorptive pads 7.Described infection index detection zone 8 is attached on the second base plate 11.
In embodiment 1-4, the immune labeled antibody of described HCG is anti-human HCG colloid gold immune labelled antibody, it is that anti-human HCG detects antibody that described HCG detects antibody, and anti-human HCG colloid gold immune labelled antibody wherein and anti-human HCG detect antibody in conjunction with the different parts of people HCG;
Described infection index detection zone 8 is provided with urine Protein Detection test paper, leucocyte Test paper, fecal occult blood Test paper and nitrite Test paper, be placed in the upper surface of base plate, and be placed on dismountable draw-in groove with HCG immunity chromatography detection test paper with horizontal coverage mode or built-in embedded mode.
Described urine Protein Detection test paper is in bromophenol blue by blank filter paper, dry processing after soaking in the solution that surfactant and citrate buffer solution form, the concentration of the bromophenol blue of telling in solution be 100-2000mg/L, the mass concentration of surfactant in solution is 0.1-10%, the pH value of citrate buffer solution is 2.8-4.6, and volumetric molar concentration is 0.01-1M;
Described leucocyte Test paper is dry processing after blank filter paper is soaked in the solution of the chloro-3-indolyl acetic acid salt of the bromo-4-of 5-and sucrose composition, the concentration of the chloro-3-indolyl acetic acid salt of the bromo-4-of described 5-in solution is 40-1000 mg/L, and the concentration of described sucrose in solution is 200-10000 mg/L;
Described fecal occult blood Test paper is at hydrogen peroxide or dicumyl peroxide by blank filter paper, tetramethyl benzidine or aminopyrine, dry processing after soaking in the solution that surfactant and PBS damping fluid form, the massfraction of described hydrogen peroxide or dicumyl peroxide is 1-10%, the concentration of tetramethyl benzidine (TMB) or aminopyrine is 0.1-40 g/L, the pH value of PBS damping fluid is 7.0, and volumetric molar concentration is 0.01-1 M;
Described nitrite Test paper is at sulfanilic acid by blank filter paper, dry processing after soaking in the solution that alpha-naphthylamine and tartrate form, the concentration of described sulfanilic acid is 500 ~ 4000 mg/L, the concentration of alpha-naphthylamine is 500 ~ 4000 mg/L, and tartaric massfraction is 0.05 ~ 10%.
If HCG immunochromatographydetecting detecting test strip C district occurs that band ，Ze T district occurs that band represents that sample is positive, does not occur that band represents feminine gender during detection; If band does not appear in C district, represent that test strips lost efficacy.Urine Protein Detection test paper color change represent undesired, color do not change represent normal.Leucocyte Test paper does not develop the color and represents normally, occurs that blue or green represents undesired.Fecal occult blood Test paper does not develop the color and represents normally, occurs that blue (or bluish violet) represents undesired.Nitrite Test paper does not develop the color and represents normally, occurs that pink or redness represent undesired.
Preparation Example 1
The antibody colloidal gold labeling method detecting for HCG is for adding 400 μ L, and 3% sal tartari regulates the pH of 50 mL colloidal gold solutions; Under stirring condition, add gradually 0.25 mL, 6.6 mg/mL anti-HCG antibody to be marked, in above-mentioned colloidal gold solution, mixes standing 30 min of room temperature; 12.5 mL confining liquids are slowly added in above-mentioned colloidal gold solution, mix, the standing 30min of room temperature; 4 ℃ of centrifugal 1h of 8000g, remove supernatant, stay loose deposits; Each pipe precipitation is dissolved with 25mL cleansing solution, and 4 ℃ of centrifugal 1h of 6000g, remove supernatant, stay loose deposits; Each pipe precipitation is dissolved with 1.2 mL cleansing solutions, proceeds in 1.5mL centrifuge tube, and 4 ℃ of centrifugal 1h of 4500g, remove supernatant; All precipitations are resuspended with the resuspended liquid of 1 mL colloidal gold antibody, 4 ℃ of preservations.
The specking method of the antibody colloidal gold pad detecting for HCG is as follows: with the resuspended liquid of colloidal gold antibody, the colloidal gold antibody bond of above-mentioned preparation is diluted to 4 times; The power supply of opening point film instrument, sets specking program, and specking amount is 8 μ L/cm; No. 1 pipeline is specking passage; No. 1 pipeline is placed in to the resuspended solution of colloidal gold antibody, selects initialize routine, 6 circulations of initialization; Gold mark pad is lain on a film instrument by fixed position, press " GO " key on control panel and start specking, after having put, take off, check the good gold mark pad of specking, the colloidal gold antibody band of specking evenly, continuously and the straight line that connects whole gold mark pad be qualified specking product, in two straight lines, occur that breakpoint is defective specking product; Often put a slice gold mark pad, " GO " key of pressing on a control panel is specking once (a slice); Specking finishes, and the gold mark pad of specking is placed in to room temperature natural drying 1 hour, on film, should can't see specking vestige.
The antibody chromatography membrane preparation method detecting for HCG is as follows: get mouse-anti people HCG antibody 500 μ g, be added in 5mL graduated centrifuge tube, antibody diluent is to 1mL, and Container Tag T indicates.Get sheep anti-mouse igg antibody 25 μ L, be added in 5 mL graduated centrifuge tubes, antibody diluent to 1 mL, Container Tag C sign; The power supply of opening point film instrument, sets specking program, and specking amount is 1 μ L/cm, and No. 1 pipeline is for detecting band specking passage, and No. 2 pipelines are contrast band specking passage; No. 1 pipeline is placed in and detects band solution, No. 2 pipelines are placed in to contrast band solution, select initialize routine, 6 circulations of initialization; Chromatographic film is lain on a film instrument by fixed position, press " GO " key on control panel and start specking, after having put, take off, check the good chromatographic film of specking, detect band and contrast band and be two evenly, continuously and the straight line that connects whole chromatographic film be qualified specking product, in two straight lines, occur that breakpoint is defective specking product; Often put a slice chromatographic film, " GO " key of pressing on a control panel is specking once (a slice); Specking finishes, and the chromatographic film of specking is placed in to room temperature natural drying 1 hour, on film, should can't see specking vestige.
The protection sheet of wider portion on base plate is removed, and edge is the coboundary of protection sheet above, will pull the chromatographic film of line, in C line mode up, is attached on base plate plate; Collaurum pad is attached to T line below, and a little contacts with NC film; Sample pad is attached to collaurum pad below, and a little contacts with collaurum pad; Then remove top protection sheet, adsorptive pads is attached to the top of NC film, a little contacts with NC film; Protection sheet and index strip paper are attached to the test strips outside assembling one by one, are assembled into kilocalorie.Connect cutting machine power supply, set the film program of cutting, setting cutting width is 4 mm; Kilocalorie certified products are kept flat in cutting machine platform track, face up, press " GO " key on guidance panel, start cutting; Often put a slice kilocalorie certified products, press on guidance panel " GO " key once, until cut all kilocalorie certified products.
Described urine Protein Detection test paper is in bromophenol blue by blank filter paper, dry processing after soaking in the solution that surfactant and citrate buffer solution form, the concentration of the bromophenol blue of telling in solution be 500 mg/L, the mass concentration of surfactant in solution is 10 %, the pH value of citrate buffer solution is 4.6, volumetric molar concentration is 0.01 M, after drying, uses cutting machine to be cut into the square that the length of side is 4 mm;
Described leucocyte Test paper is dry processing after blank filter paper is soaked in the solution of the chloro-3-indolyl acetic acid salt of the bromo-4-of 5-and sucrose composition, the concentration of the chloro-3-indolyl acetic acid salt of the bromo-4-of described 5-in solution is 800 mg/L, the concentration of described sucrose in solution is 10000 mg/L, after drying, uses cutting machine to be cut into the square that the length of side is 4 mm;
Described fecal occult blood Test paper is at hydrogen peroxide or dicumyl peroxide by blank filter paper, tetramethyl benzidine or aminopyrine, dry processing after soaking in the solution that surfactant and PBS damping fluid form, the massfraction of described hydrogen peroxide or dicumyl peroxide is 10%, the concentration of tetramethyl benzidine (TMB) or aminopyrine is 40 g/L, the pH value of PBS damping fluid is 7.0, and volumetric molar concentration is 0.01 M, after drying, uses cutting machine to be cut into the square that the length of side is 4 mm;
Described nitrite Test paper is at sulfanilic acid by blank filter paper, dry processing after soaking in the solution that alpha-naphthylamine and tartrate form, the concentration of described sulfanilic acid is 4000 mg/L, the concentration of alpha-naphthylamine is 2000 mg/L, tartaric massfraction is 0.05%, after drying, uses cutting machine to be cut into the square that the length of side is 4 mm.
To urinate Protein Detection test paper respectively, leucocyte Test paper, one or more in fecal occult blood Test paper and nitrite Test paper cover with level or built-in embedded mode is pasted and is fixed on transparent PVC sheet, and each test paper spacing is 5 mm, are cut into the length of side and are the test strips of 4 mm.
Preparation Example 2
The antibody colloidal gold labeling method detecting for HCG is for adding 400 μ L, and 3% sal tartari regulates the pH of 50 ml colloidal gold solutions; Under stirring condition, add gradually 0.25 mL, 6.6 mg/mL anti-HCG antibody to be marked, in above-mentioned colloidal gold solution, mixes standing 30 min of room temperature; 12.5 mL confining liquids are slowly added in above-mentioned colloidal gold solution, mix, the standing 30min of room temperature; 4 ℃ of centrifugal 1h of 8000g, remove supernatant, stay loose deposits; Each pipe precipitation is dissolved with 25ml cleansing solution, and 4 ℃ of centrifugal 1h of 6000g, remove supernatant, stay loose deposits; Each pipe precipitation is dissolved with 1.2mL cleansing solution, proceeds in 1.5mL centrifuge tube, and 4 ℃ of centrifugal 1h of 4500g, remove supernatant; All precipitations are resuspended with the resuspended liquid of 1 mL colloidal gold antibody, 4 ℃ of preservations.
The specking method of the antibody colloidal gold pad detecting for HCG is as follows: with the resuspended liquid of colloidal gold antibody, the colloidal gold antibody bond of above-mentioned preparation is diluted to 4 times; The power supply of opening point film instrument, sets specking program, and specking amount is 8 μ L/cm; No. 1 pipeline is specking passage; No. 1 pipeline is placed in to the resuspended solution of colloidal gold antibody, selects initialize routine, 6 circulations of initialization; Gold mark pad is lain on a film instrument by fixed position, press " GO " key on control panel and start specking, after having put, take off, check the good gold mark pad of specking, the colloidal gold antibody band of specking evenly, continuously and the straight line that connects whole gold mark pad be qualified specking product, in two straight lines, occur that breakpoint is defective specking product; Often put a slice gold mark pad, " GO " key of pressing on a control panel is specking once (a slice); Specking finishes, and the gold mark pad of specking is placed in to room temperature natural drying 1 hour, on film, should can't see specking vestige.
The antibody chromatography membrane preparation method detecting for HCG is as follows: get mouse-anti people HCG antibody 500 μ g, be added in 5mL graduated centrifuge tube, antibody diluent is to 1mL, and Container Tag T indicates.Get sheep anti-mouse igg antibody 25 μ L, be added in 5mL graduated centrifuge tube, antibody diluent indicates to 1mL Container Tag C; The power supply of opening point film instrument, sets specking program, and specking amount is 1 μ L/cm, and No. 1 pipeline is for detecting band specking passage, and No. 2 pipelines are contrast band specking passage; No. 1 pipeline is placed in and detects band solution, No. 2 pipelines are placed in to contrast band solution, select initialize routine, 6 circulations of initialization; Chromatographic film is lain on a film instrument by fixed position, press " GO " key on control panel and start specking, after having put, take off, check the good chromatographic film of specking, detect band and contrast band and be two evenly, continuously and the straight line that connects whole chromatographic film be qualified specking product, in two straight lines, occur that breakpoint is defective specking product; Often put a slice chromatographic film, " GO " key of pressing on a control panel is specking once (a slice); Specking finishes, and the chromatographic film of specking is placed in to room temperature natural drying 1 hour, on film, should can't see specking vestige.
The protection sheet of wider portion on base plate is removed, and edge is the coboundary of protection sheet above, will pull the chromatographic film of line, in C line mode up, is attached on base plate plate; Collaurum pad is attached to T line below, and a little contacts with NC film; Sample pad is attached to collaurum pad below, and a little contacts with collaurum pad; Then remove top protection sheet, adsorptive pads is attached to the top of NC film, a little contacts with NC film; Protection sheet and index strip paper are attached to the test strips outside assembling one by one, are assembled into kilocalorie.Connect cutting machine power supply, set the film program of cutting, setting cutting width is 4 mm; Kilocalorie certified products are kept flat in cutting machine platform track, face up, press " GO " key on guidance panel, start cutting; Often put a slice kilocalorie certified products, press on guidance panel " GO " key once, until cut all kilocalorie certified products.
Described urine Protein Detection test paper is in bromophenol blue by blank filter paper, dry processing after soaking in the solution that surfactant and citrate buffer solution form, the concentration of the bromophenol blue of telling in solution be 500 mg/L, the concentration of surfactant in solution is 10 %, the pH value of citrate buffer solution is 4.6, volumetric molar concentration is 0.01 M, after drying, uses cutting machine to be cut into the square that the length of side is 4 mm;
Described leucocyte Test paper is dry processing after blank filter paper is soaked in the solution of the chloro-3-indolyl acetic acid salt of the bromo-4-of 5-and sucrose composition, the concentration of the chloro-3-indolyl acetic acid salt of the bromo-4-of described 5-in solution is 800 mg/L, the concentration of described sucrose in solution is 10000 mg/L, after drying, uses cutting machine to be cut into the square that the length of side is 4 mm;
Described fecal occult blood Test paper is at hydrogen peroxide or dicumyl peroxide by blank filter paper, tetramethyl benzidine or aminopyrine, dry processing after soaking in the solution that surfactant and PBS damping fluid form, the massfraction of described hydrogen peroxide or dicumyl peroxide is 10%, the concentration of tetramethyl benzidine (TMB) or aminopyrine is 40 g/L, the pH value of PBS damping fluid is 7.0, and volumetric molar concentration is 0.01 M, after drying, uses cutting machine to be cut into the square that the length of side is 4 mm;
Described nitrite Test paper is at sulfanilic acid by blank filter paper, dry processing after soaking in the solution that alpha-naphthylamine and tartrate form, the concentration of described sulfanilic acid is 4000 mg/L, the concentration of alpha-naphthylamine is 2000 mg/L, tartaric massfraction is 0.05%, after drying, uses cutting machine to be cut into the square that the length of side is 4 mm.
To urinate Protein Detection test paper respectively, leucocyte Test paper, one or more in fecal occult blood Test paper and nitrite Test paper cover with level or the stickup of built-in embedded mode is fixed on transparent PVC sheet, each test paper spacing is 5mm, be cut into the length of side and be the test strips of 4mm, and be placed on draw-in groove side by side with HCG immunochromatographydetecting detecting test strip.
Preparation Example 3
The antibody colloidal gold labeling method detecting for HCG is for adding 400 μ L, and 3% sal tartari regulates the pH of 50 mL colloidal gold solutions; Under stirring condition, add gradually 0.25mL, 6.6 mg/mL anti-HCG antibody to be marked, in above-mentioned colloidal gold solution, mixes standing 30 min of room temperature; 12.5 mL confining liquids are slowly added in above-mentioned colloidal gold solution, mix, the standing 30min of room temperature; 4 ℃ of centrifugal 1h of 8000g, remove supernatant, stay loose deposits; Each pipe precipitation is dissolved with 25mL cleansing solution, and the centrifugal 1h of 6,000 4 ℃ of g, removes supernatant, stays loose deposits; Each pipe precipitation is dissolved with 1.2mL cleansing solution, proceeds in 1.5mL centrifuge tube, and 4 ℃ of centrifugal 1h of 4500g, remove supernatant; All precipitations are resuspended with the resuspended liquid of 1mL colloidal gold antibody, 4 ℃ of preservations.
The specking method of the antibody colloidal gold pad detecting for HCG is as follows: with the resuspended liquid of colloidal gold antibody, the colloidal gold antibody bond of above-mentioned preparation is diluted to 4 times; The power supply of opening point film instrument, sets specking program, and specking amount is 8 μ L/cm; No. 1 pipeline is specking passage; No. 1 pipeline is placed in to the resuspended solution of colloidal gold antibody, selects initialize routine, 6 circulations of initialization; Gold mark pad is lain on a film instrument by fixed position, press " GO " key on control panel and start specking, after having put, take off, check the good gold mark pad of specking, the colloidal gold antibody band of specking evenly, continuously and the straight line that connects whole gold mark pad be qualified specking product, in two straight lines, occur that breakpoint is defective specking product; Often put a slice gold mark pad, " GO " key of pressing on a control panel is specking once (a slice); Specking finishes, and the gold mark pad of specking is placed in to room temperature natural drying 1 hour, on film, should can't see specking vestige.
The antibody chromatography membrane preparation method detecting for HCG is as follows: get mouse-anti people HCG antibody 500 μ g, be added in 5mL graduated centrifuge tube, antibody diluent is to 1mL, and Container Tag T indicates.Get sheep anti-mouse igg antibody 25 μ L, be added in 5mL graduated centrifuge tube, antibody diluent is to 1mL, and Container Tag C indicates; The power supply of opening point film instrument, sets specking program, and specking amount is 1 μ L/cm, and No. 1 pipeline is for detecting band specking passage, and No. 2 pipelines are contrast band specking passage; No. 1 pipeline is placed in and detects band solution, No. 2 pipelines are placed in to contrast band solution, select initialize routine, 6 circulations of initialization; Chromatographic film is lain on a film instrument by fixed position, press " GO " key on control panel and start specking, after having put, take off, check the good chromatographic film of specking, detect band and contrast band and be two evenly, continuously and the straight line that connects whole chromatographic film be qualified specking product, in two straight lines, occur that breakpoint is defective specking product; Often put a slice chromatographic film, " GO " key of pressing on a control panel is specking once (a slice); Specking finishes, and the chromatographic film of specking is placed in to room temperature natural drying 1 hour, on film, should can't see specking vestige.
The protection sheet of wider portion on base plate is removed, and edge is the coboundary of protection sheet above, will pull the chromatographic film of line, in C line mode up, is attached on base plate plate; Collaurum pad is attached to T line below, and a little contacts with NC film; Sample pad is attached to collaurum pad below, and a little contacts with collaurum pad; Then remove top protection sheet, adsorptive pads is attached to the top of NC film, a little contacts with NC film; Protection sheet and index strip paper are attached to the test strips outside assembling one by one, are assembled into kilocalorie.Connect cutting machine power supply, set the film program of cutting, setting cutting width is 4 mm; Kilocalorie certified products are kept flat in cutting machine platform track, face up, press " GO " key on guidance panel, start cutting; Often put a slice kilocalorie certified products, press on guidance panel " GO " key once, until cut all kilocalorie certified products.
Described urine Protein Detection test paper is in bromophenol blue by blank filter paper, dry processing after soaking in the solution that surfactant and citrate buffer solution form, the concentration of the bromophenol blue of telling in solution be 500 mg/L, the concentration of surfactant in solution is 10 %, the pH value of citrate buffer solution is 4.6, volumetric molar concentration is 0.01 M, after drying, uses cutting machine to be cut into the square that the length of side is 4 mm;
Described leucocyte Test paper is dry processing after blank filter paper is soaked in the solution of the chloro-3-indolyl acetic acid salt of the bromo-4-of 5-and sucrose composition, the concentration of the chloro-3-indolyl acetic acid salt of the bromo-4-of described 5-in solution is 800 mg/L, the concentration of described sucrose in solution is 10000 mg/L, after drying, uses cutting machine to be cut into the square that the length of side is 4 mm;
Described fecal occult blood Test paper is at hydrogen peroxide or dicumyl peroxide by blank filter paper, tetramethyl benzidine or aminopyrine, dry processing after soaking in the solution that surfactant and PBS damping fluid form, the massfraction of described hydrogen peroxide or dicumyl peroxide is 10%, the concentration of tetramethyl benzidine (TMB) or aminopyrine is 40 g/L, the pH value of PBS damping fluid is 7.0, and volumetric molar concentration is 0.01 M, after drying, uses cutting machine to be cut into the square that the length of side is 4 mm;
Described nitrite Test paper is at sulfanilic acid by blank filter paper, dry processing after soaking in the solution that alpha-naphthylamine and tartrate form, the concentration of described sulfanilic acid is 4000 mg/L, the concentration of alpha-naphthylamine is 2000 mg/L, tartaric massfraction is 0.05%, after drying, uses cutting machine to be cut into the square that the length of side is 4 mm.
To urinate Protein Detection test paper respectively, leucocyte Test paper, one or more in fecal occult blood Test paper and nitrite Test paper cover with level or the stickup of built-in embedded mode is fixed on transparent PVC sheet, each test paper spacing is 5 mm, be cut into the length of side and be the test strips of 4 mm, and be placed in respectively the upper and lower surface of base plate with HCG immunochromatographydetecting detecting test strip.
Preparation Example 4
The antibody colloidal gold labeling method detecting for HCG is for adding 400 μ L, and 3% sal tartari regulates the pH of 50 mL colloidal gold solutions; Under stirring condition, add gradually 0.25 mL, 6.6 mg/mL anti-HCG antibody to be marked, in above-mentioned colloidal gold solution, mixes standing 30 min of room temperature; 12.5mL confining liquid is slowly added in above-mentioned colloidal gold solution, mix, the standing 30min of room temperature; 4 ℃ of centrifugal 1h of 8000g, remove supernatant, stay loose deposits; Each pipe precipitation is dissolved with 25mL cleansing solution, and 4 ℃ of centrifugal 1h of 6000g, remove supernatant, stay loose deposits; Each pipe precipitation is dissolved with 1.2 mL cleansing solutions, proceeds in 1.5 mL centrifuge tubes, and 4 ℃ of centrifugal 1h of 4500g, remove supernatant; All precipitations are resuspended with the resuspended liquid of 1mL colloidal gold antibody, 4 ℃ of preservations.
The specking method of the antibody colloidal gold pad detecting for HCG is as follows: with the resuspended liquid of colloidal gold antibody, the colloidal gold antibody bond of above-mentioned preparation is diluted to 4 times; The power supply of opening point film instrument, sets specking program, and specking amount is 8 μ L/cm; No. 1 pipeline is specking passage; No. 1 pipeline is placed in to the resuspended solution of colloidal gold antibody, selects initialize routine, 6 circulations of initialization; Gold mark pad is lain on a film instrument by fixed position, press " GO " key on control panel and start specking, after having put, take off, check the good gold mark pad of specking, the colloidal gold antibody band of specking evenly, continuously and the straight line that connects whole gold mark pad be qualified specking product, in two straight lines, occur that breakpoint is defective specking product; Often put a slice gold mark pad, " GO " key of pressing on a control panel is specking once (a slice); Specking finishes, and the gold mark pad of specking is placed in to room temperature natural drying 1 hour, on film, should can't see specking vestige.
The antibody chromatography membrane preparation method detecting for HCG is as follows: get mouse-anti people HCG antibody 500 ug, be added in 5 ml graduated centrifuge tubes, antibody diluent to 1 ml, Container Tag T sign.Get sheep anti-mouse igg antibody 25 μ L, be added in 5mL graduated centrifuge tube, antibody diluent to 1 mL, Container Tag C sign; The power supply of opening point film instrument, sets specking program, and specking amount is 1 μ L/cm, and No. 1 pipeline is for detecting band specking passage, and No. 2 pipelines are contrast band specking passage; No. 1 pipeline is placed in and detects band solution, No. 2 pipelines are placed in to contrast band solution, select initialize routine, 6 circulations of initialization; Chromatographic film is lain on a film instrument by fixed position, press " GO " key on control panel and start specking, after having put, take off, check the good chromatographic film of specking, detect band and contrast band and be two evenly, continuously and the straight line that connects whole chromatographic film be qualified specking product, in two straight lines, occur that breakpoint is defective specking product; Often put a slice chromatographic film, " GO " key of pressing on a control panel is specking once (a slice); Specking finishes, and the chromatographic film of specking is placed in to room temperature natural drying 1 hour, on film, should can't see specking vestige.
The protection sheet of wider portion on base plate is removed, and edge is the coboundary of protection sheet above, will pull the chromatographic film of line, in C line mode up, is attached on base plate plate; Collaurum pad is attached to T line below, and a little contacts with NC film; Sample pad is attached to collaurum pad below, and a little contacts with collaurum pad; Then remove top protection sheet, adsorptive pads is attached to the top of NC film, a little contacts with NC film; Protection sheet and index strip paper are attached to the test strips outside assembling one by one, are assembled into kilocalorie.Connect cutting machine power supply, set and cut film program, setting cutting width is 4mm; Kilocalorie certified products are kept flat in cutting machine platform track, face up, press " GO " key on guidance panel, start cutting; Often put a slice kilocalorie certified products, press on guidance panel " GO " key once, until cut all kilocalorie certified products.
Described urine Protein Detection test paper is in bromophenol blue by blank filter paper, dry processing after soaking in the solution that surfactant and citrate buffer solution form, the concentration of the bromophenol blue of telling in solution be 500 mg/L, the concentration of surfactant in solution is 10 %, the pH value of citrate buffer solution is 4.6, volumetric molar concentration is 0.01 M, after drying, uses cutting machine to be cut into the square that the length of side is 4 mm;
Described leucocyte Test paper is dry processing after blank filter paper is soaked in the solution of the chloro-3-indolyl acetic acid salt of the bromo-4-of 5-and sucrose composition, the concentration of the chloro-3-indolyl acetic acid salt of the bromo-4-of described 5-in solution is 800 mg/L, the concentration of described sucrose in solution is 10000 mg/L, after drying, uses cutting machine to be cut into the square that the length of side is 4mm;
Described fecal occult blood Test paper is at hydrogen peroxide or dicumyl peroxide by blank filter paper, tetramethyl benzidine or aminopyrine, dry processing after soaking in the solution that surfactant and PBS damping fluid form, the massfraction of described hydrogen peroxide or dicumyl peroxide is 10%, the concentration of tetramethyl benzidine (TMB) or aminopyrine is 40g/L, the pH value of PBS damping fluid is 7.0, and volumetric molar concentration is 0.01M, after drying, uses cutting machine to be cut into the square that the length of side is 4mm;
Described nitrite Test paper is at sulfanilic acid by blank filter paper, dry processing after soaking in the solution that alpha-naphthylamine and tartrate form, the concentration of described sulfanilic acid is 4000 mg/L, the concentration of alpha-naphthylamine is 2000mg/L, tartaric massfraction is 0.05%, after drying, uses cutting machine to be cut into the square that the length of side is 4 mm.
To urinate Protein Detection test paper respectively, leucocyte Test paper, one or more in fecal occult blood Test paper and nitrite Test paper cover with level or the stickup of built-in embedded mode is fixed on transparent PVC sheet, each test paper spacing is 5mm, be cut into the length of side and be the test strips of 4mm, and be placed on dismountable draw-in groove with HCG immuno-chromatographic test paper strip.
Claims (9)
1. a human chorionic gonadotrophin and urinary system infection contamination joint inspection kit, comprise the first base plate (10), it is characterized in that: in the one side of the first base plate (10), be provided with chromatographic film (6), the front end of chromatographic film (6) arranges opaque diaphragm (3), and the front end of opaque diaphragm (3) arranges arrow (2); The front end of arrow (2) arranges pad (1); In chromatographic film (6), be also disposed with detection line (4) and nature controlling line (5), chromatographic film (6) rear end is provided with adsorptive pads (7).
2. human chorionic gonadotrophin and urinary system infection contamination joint inspection kit as claimed in claim 1, is characterized in that: also comprise infection index detection zone (8).
3. human chorionic gonadotrophin and urinary system infection contamination joint inspection kit as claimed in claim 2, is characterized in that: it is upper or be compound on any one surface of the first base plate (10) that described infection index detection zone (8) is attached to the second base plate (11).
4. a human chorionic gonadotrophin and urinary system infection contamination joint inspection kit, comprise the first base plate (10), it is characterized in that: in the one side of the first base plate (10), be provided with chromatographic film (6), the front end of chromatographic film (6) arranges pad (1), and the place ahead of pad (1) also arranges sample pad (9); In chromatographic film (6), be also disposed with detection line (4) and nature controlling line (5), chromatographic film (6) rear end is provided with adsorptive pads (7); On the lower surface of the first base plate (10), be also provided with infection index detection zone (8).
5. human chorionic gonadotrophin and urinary system infection contamination joint inspection kit as described in claim 2 or 4, it is characterized in that: described infection index detection zone (8) is provided with urine Protein Detection test paper, leucocyte Test paper, one or more in fecal occult blood Test paper and nitrite Test paper.
6. human chorionic gonadotrophin and urinary system infection contamination joint inspection kit as claimed in claim 5, it is characterized in that: described urine Protein Detection test paper, leucocyte Test paper, first base plate (10) of fecal occult blood Test paper and nitrite Test paper or the material of the second base plate (11) are a kind of in PET, PVC, PE, PP or PS, long is 10-300mm, wide 2-30mm, thickness is 0.1-1.5mm.
7. human chorionic gonadotrophin and urinary system infection contamination joint inspection kit as claimed in claim 5, it is characterized in that: described urine Protein Detection test paper, leucocyte Test paper, the basic material of fecal occult blood Test paper and nitrite Test paper is cellulose filter paper, glass fiber filter paper, chromatographic paper, polyester film or nylon membrane; Described test paper be shaped as square, rectangle, circle, ellipse, triangle, rhombus or trapezoidal; Size is long 1-30 mm; Wide 1-30 mm; Thickness 0.01-5 mm; Spacing between multiple test paper is 1-30 mm.
8. human chorionic gonadotrophin and urinary system infection contamination joint inspection kit as described in claim 1 or 4, is characterized in that: on described pad (1), be coated with human chorionic gonadotrophin HCG and detect antibody.
9. human chorionic gonadotrophin and urinary system infection contamination joint inspection kit as described in claim 1 or 4, is characterized in that: in described chromatographic film (6), be coated with HCG and detect antibody.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108139410A (en) * | 2015-04-02 | 2018-06-08 | 瑞士斯保德精密诊断有限公司 | Improved Pregnancy Test device and method |
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| CN203616319U (en) * | 2013-11-04 | 2014-05-28 | 无锡博慧斯生物医药科技有限公司 | HCG (human chorionic gonadotropin) and UTI (urinary tract infection) joint detection kit |
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