CN103535328B - Method for industrially breeding phyoseiulus persimilis - Google Patents
Method for industrially breeding phyoseiulus persimilis Download PDFInfo
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Abstract
The invention relates to a method for industrially breeding phyoseiulus persimilis. The method includes steps of A, constructing an industrial breeding greenhouse, specifically, dividing the greenhouse into a phyoseiulus persimilis food-chain tetranychus urticae breeding area, a tetranychus urticae food-chain clean seedling planting area and a phyoseiulus persimilis isolation breeding area; B, planting clean seedlings, in other words, cultivating peanuts in nutrition bowls, and keeping the temperature of the clean seedling planting area to range from 18 DEG C to 35 DEG C and the humidity of the clean seedling planting area to range from 35% to 85%; C, breeding tetranychus urticae, in other words, breeding a large quantity of tetranychus urticae in the greenhouse and performing conservation breeding on the tetranychus urticae in small climatic chambers synchronously; D, breeding the phyoseiulus persimilis in an isolation manner. The peanuts are selected to be used as food for the tetranychus urticae. The method has the advantages that the method aims to solve the problem that a large quantity of phyoseiulus persimilis cannot be bread all the year round, the phyoseiulus persimilis can be bred by means of cultivating host plants and breeding host insects and natural enemy insects in the glass greenhouse all the year round under full automatic control, so that purposes of low energy consumption, high efficiency and long-term output can be achieved.
Description
Technical field
The present invention relates to beneficial insect factorial praluction and pest technical field of biological control, particularly a kind of method of industrialized propagation Phyloseiulus nersimilis.
Background technology
Along with the aggravation of mankind's activity; the increase of the pressure of grain, population, natural resources; ball ecological environment worsens year by year with unprecedented speed, and survival crisis is deepened day by day, and " economic sustainable development and the sustainable use of resource " policy receives publicity.The strategy of Pest Management also will note preventing the atrophy of natural ecosystems and impaired, to adapt to the needs (Ding Yanqin, 2005) of the mankind, environment long-term interest.At present, evil mite harms the crops and the important pests of fruit tree, can endanger the multiclass crops such as eggplant, cotton, strawberry, legume crop, apple, oranges and tangerines, bring massive losses to the production of peasant.Based on the green prevention and control technology of Phytoseiulus Persimilis evil mite, be subject to the great attention of all circles.
In the production application of artificial breeding Phyloseiulus nersimilis, for different facility types, different cultivation season, there is problems such as breeding not in time, breeding amount is inadequate, only had and realize high efficiency continuous breeding and could solve problems." a kind of propagation method (patent No. 200810118205.4) of Phyloseiulus nersimilis " of Beijing City Agriculture and Forestry Institute application, does not relate to workshop, greenhouse and facility condition; Tetranychus urticae greenhouse mass rearing and microclimate room conservation are bred; The aspects such as Phyloseiulus nersimilis breeding isolation.
Summary of the invention
The object of the invention is to can not mass rearing problem all the year round for Phyloseiulus nersimilis, provides a kind ofly to utilize the method for cultivating host plant, host egg insect and natural enemy insect the full self-controlled greenhouse anniversary, realizes low energy consumption, high efficiency, all the year round output.
This industrialized propagation Phyloseiulus nersimilis method, realizes as follows:
A, construction plant modification greenhouse: greenhouse is divided into Phyloseiulus nersimilis food chain Tetranychus urticae multiplication plot, and Tetranychus urticae food chain cleans seedling growing area, and Phyloseiulus nersimilis isolation multiplication plot;
B, clean seed are planted: selectively beans are as the food of Tetranychus urticae, cultivate in nutritive cube, clean seedling growing area temperature 18-35 DEG C, humidity 35%-85%;
C, Tetranychus urticae are bred: synchronously carry out Tetranychus urticae greenhouse mass rearing and microclimate room conservation is bred, part mass rearing in greenhouse, and another part is bred in microclimate room conservation; Microclimate room remains purer Phyloseiulus nersimilis breeding, in order to regular replenishment greenhouse Tetranychus urticae propagating population;
D, Phyloseiulus nersimilis isolation breeding: Phyloseiulus nersimilis plant propagation greenhouse independently becomes a greenhouse with clean seedling district separately with greenhouse, tetranychid district, and more than 20 meters distances of being separated by, avoid Phyloseiulus nersimilis to import Tetranychus urticae multiplication plot into, affect tetranychid and normally breed.
Described industrialized propagation Phyloseiulus nersimilis method, greenhouse described in steps A and subregion all have strict seal, greenhouse arranges roof window ventilating opening, 80 order fly nets are all installed on each ventilating opening and both sides, corridor, cooling adopts, and " wet curtain and blower fan " combines and air-conditioning, heats and adopts circular wing type heating installation and air-conditioning.
Described industrialized propagation Phyloseiulus nersimilis method, the greenhouse described in steps A and subregion cooling point four grades, 1. during ambient temperature 25-28 DEG C, only open roof window gravity-flow ventilation; 2., during extraneous 29-32 DEG C, roof window and blower fan is driven; 3., during ambient temperature 33-35 DEG C, wet curtain and blower fan is driven; 4., during ambient temperature more than 36 DEG C, turn on the aircondition; Heat up and adopt heating installation, if when temperature is still lower than 18 DEG C, auxiliary heating of turning on the aircondition.
Described industrialized propagation Phyloseiulus nersimilis method, clean bean seedlings plantation described in step B comprises, potting soil is put into the nutritive cube of medium size 16cm × 14cm, in each nutritive cube, the ground beans of having planted are put on seedbed, are plugged automatic drip irrigation watering facility by plantation 3 ground beans seed in order, a drip irrigation pipe inserted by every basin, water a water every three days, notice that irrigation amount is too much unsuitable, keep soil from packing together; After emerging, watered a chemical fertilizer water every about 10 days, strengthen management, cultivate healthy and strong bean seedlings.
Described industrialized propagation Phyloseiulus nersimilis method, Tetranychus urticae greenhouse mass rearing described in step C is for expanding numerous and inoculation Phyloseiulus nersimilis, method comprises, potted plant for a part fresh healthy and strong bean seedlings are moved into Tetranychus urticae and breeds workshop, every basin inoculation 3-4 sheet is with the blade of tetranychid, on every sheet leaf, the amount of Tetranychus urticae is 50-100 head, when Tetranychus urticae is transferred on new blade, clears up carrier blade in time; Tetranychus urticae greenhouse multiplication plot temperature 18-32 DEG C, humidity 35%-75%.
Described industrialized propagation Phyloseiulus nersimilis method, microclimate room conservation breeding Tetranychus urticae described in step C, adopts the breeding of bean seedlings inoculation Tetranychus urticae, is cut by partial blade as inoculation carrier inoculation new talent after 5-7 days, remainingly bring greenhouse into, supplement its Tetranychus urticae propagating population.
Described industrialized propagation Phyloseiulus nersimilis method, described microclimate room surrounding is that glass curtain wall is beneficial to solar radiation, arranges external door, inside door isolation, inner outfit air-conditioning, light compensating lamp, dehumidifier, by automatic controlling system, temperature 20-31 DEG C, humidity 35%-55%.
Described industrialized propagation Phyloseiulus nersimilis method, Phyloseiulus nersimilis isolation breeding described in step D comprises: when the amount of every basin bean seedlings Tetranychus urticae is 800-1000 head, move the Phyloseiulus nersimilis room of breeding inoculation Predatory Mites to, select the plant leaf containing a large amount of Phyloseiulus nersimilis, cut gently with scissors, be placed in pallet as inoculation carrier, then with tweezers gently gripping blade be placed directly on bean seedlings and inoculate, Phyloseiulus nersimilis can be climbed to very soon on bean seedlings and take food Tetranychus urticae, and inoculative proportion is that 1:40-50 is advisable.
Described industrialized propagation Phyloseiulus nersimilis method, run away for preventing Phyloseiulus nersimilis in Phyloseiulus nersimilis isolation multiplication plot, breeding of Phyloseiulus nersimilis adopts movable seedbed to place, arrange at rectangle tank central authorities " one " word, distance tank dual-side edge is 35cm, water logging in anti-water stopping trough there is not plant, and potted plant heelpiece goes out the water surface; When breeding Phyloseiulus nersimilis more than 40,000 every day, 10-30 mu facilities vegetable tetranychid biological control demand can be met.
Technological progress effect of the present invention shows:
(1) specify that greenhouse and the appointed condition of breeding Phyloseiulus nersimilis, adopt " cascade+blower fan " combined type to substitute air-conditioning temperature-reducing, greatly reduce energy resource consumption; Each link all has strict quarantine measures, solves different subregion insect and mutually flees interference problem.
(2) synchronously carry out Tetranychus urticae greenhouse mass rearing and microclimate room conservation is bred, phytotron can keep purer Tetranychus urticae all the time, in order to supplementing greenhouse Tetranychus urticae propagating population, improves the annual quantity-produced stability of Tetranychus urticae.
(3) the cultured space of Phyloseiulus nersimilis is comparatively independent, strictly separates, prevent two kinds of insects from running away mutually, affect feeding efficiency with the long distance in the Tetranychus urticae room of breeding.
Accompanying drawing explanation
Fig. 1 is the plane figure schematic diagram of greenhouse subregion
Fig. 2 is Phyloseiulus nersimilis propagation tank floor map
In figure:
1, clean seedling district 2, Tetranychus urticae multiplication plot 3, corridor 4, cascade 5 blower fan
6, Phyloseiulus nersimilis multiplication plot 7, isolation strip 6-1, Phyloseiulus nersimilis greenhouse
6-2, movable seedbed 6-3, rectangle tank 6-4, Potted orchard
Embodiment
Further illustrate for embodiment with Phyloseiulus nersimilis industrialized propagation in year September prebiotic anti-Co., Ltd in Hengshui City field in September, 2009 to 2013 below:
Tetranychus urticae (greenhouse, Hengshui City Taocheng District, Hebei province gathers, host's eggplant)
Phyloseiulus nersimilis (Beijing City Academy Of Agriculture and Forest Sciences INST OF PLANT PROT & ENVIRONME provides)
1, workshop, greenhouse and facility condition: as shown in Figure 1, workshop, greenhouse is divided into clean seedling and cultivates district 1, Tetranychus urticae multiplication plot 2, Phyloseiulus nersimilis multiplication plot 6 three parts.Increase glass partition at not same district intersection, cascade 4 heat sink and fly net are installed in both sides, corridor, the blower fan 5 that configuration is ventilated and cooling, heating device.Greenhouse and subregion all have strict seal, and greenhouse arranges roof window ventilating opening, and 80 order fly nets are all installed on each ventilating opening and both sides, corridor, and cooling adopts, and " wet curtain and blower fan " combines and air-conditioning, heats and adopts circular wing type heating installation and air-conditioning.
Greenhouse and subregion cooling point four grades, 1. during ambient temperature 25-28 DEG C, only open roof window gravity-flow ventilation; 2., during extraneous 29-32 DEG C, roof window and blower fan is driven; 3., during ambient temperature 33-35 DEG C, wet curtain and blower fan is driven; 4., during ambient temperature more than 36 DEG C, turn on the aircondition; Heat up and adopt heating installation, if when temperature is still lower than 18 DEG C, auxiliary heating of turning on the aircondition.
2, clean seed is planted: selectively beans are as the food of Tetranychus urticae, cultivate in nutritive cube, clean seedling growing area temperature 18-35 DEG C, humidity 35%-85%;
Every day, potted plant 65 basin bean seedlings, put into medium size nutritive cube (16cm × 14cm) by potting soil, plantation 3 ground beans seed in each nutritive cube.Put in order on seedbed by the ground beans of having planted, plug automatic drip irrigation watering facility, a drip irrigation pipe inserted by every basin, waters a water every three days, notices that irrigation amount is too much unsuitable, keeps soil from packing together; After emerging, watered a chemical fertilizer water every about 10 days, strengthen management, cultivate healthy and strong bean seedlings.
3, Tetranychus urticae is bred: synchronously carry out Tetranychus urticae greenhouse mass rearing and microclimate room conservation is bred, part mass rearing in greenhouse, and another part is bred in microclimate room conservation; Secondary climatic chamber remains purer Tetranychus urticae, in order to regular replenishment greenhouse Tetranychus urticae propagating population;
In 3-1, greenhouse, the method for mass rearing Tetranychus urticae is: every day picking pinch after potted plant bean seedlings, in greenhouse, carry out mass rearing Tetranychus urticae.Method is that plant that picking is loaded with Tetranychus urticae 50-100 head/leaf is moved Phyloseiulus nersimilis to and breeds district 2, clip is placed in plate with about 200, the blade (50-100 head/leaf) of tetranychid, with tweezers gripping blade gently, be placed on fresh bean seedlings, every basin puts 3-4 sheet leaf.
Breed to be conducive to Tetranychus urticae, preferred control temperature is 20-31 DEG C, humidity 35%-55%, and when temperature is higher or lower than design temperature, computer automatically starts or closes air discharging facility.
3-2, microclimate room conservation Tetranychus urticae are bred, and adopt bean seedlings inoculation to be cut by partial blade as inoculation carrier inoculation new talent after 5-7 days, remainingly bring greenhouse into, supplement its Tetranychus urticae propagating population.
Microclimate room surrounding is that glass curtain wall is beneficial to solar radiation, arranges external door, inside door isolation, inner outfit air-conditioning, light compensating lamp, dehumidifier, by automatic controlling system, and temperature 20-31 DEG C, humidity 35%-55%.
4, Phyloseiulus nersimilis batch production is bred in enormous quantities:
When the amount of every basin bean seedlings Tetranychus urticae is 800-1000 head, move the Phyloseiulus nersimilis room of breeding inoculation Predatory Mites-Phyloseiulus nersimilis to, select the plant leaf containing a large amount of Phyloseiulus nersimilis, cut gently with scissors, be placed in pallet as inoculation carrier, then with tweezers gently gripping blade be placed directly on bean seedlings and inoculate, Phyloseiulus nersimilis can be climbed to very soon on bean seedlings and take food Tetranychus urticae, and inoculative proportion is that 1:40-50 is advisable.
For preventing Phyloseiulus nersimilis from running away, Phyloseiulus nersimilis plant propagation greenhouse independently becomes a greenhouse with clean seedling district separately with greenhouse, tetranychid district, and more than 20 meters distances of being separated by, avoid Phyloseiulus nersimilis to import Tetranychus urticae multiplication plot into, affect tetranychid and normally breed.
As shown in Figure 2, the multiplication plot 6 of Phyloseiulus nersimilis is bred and is adopted movable seedbed 2 to place, and arranges at rectangle tank 3 central authorities " one " word, and distance tank dual-side is along being 35cm, and the water logging in anti-water stopping trough there is not plant, and potted plant heelpiece goes out the water surface; When breeding Phyloseiulus nersimilis more than 40,000 every day, 10-30 mu facilities vegetable tetranychid biological control demand can be met.
Claims (6)
1. an industrialized propagation Phyloseiulus nersimilis method, its feature comprises the steps:
A, construction plant modification greenhouse: greenhouse is divided into Phyloseiulus nersimilis food chain Tetranychus urticae multiplication plot, and Tetranychus urticae food chain cleans seedling growing area, and Phyloseiulus nersimilis isolation multiplication plot;
B, clean seed are planted: selectively beans are as the food of Tetranychus urticae, cultivate in nutritive cube, clean seedling growing area temperature 18-35 DEG C, humidity 35%-85%;
C, Tetranychus urticae are bred: synchronously carry out Tetranychus urticae greenhouse mass rearing and microclimate room conservation is bred, part mass rearing in greenhouse, and another part is bred in microclimate room conservation; Microclimate room remains purer Tetranychus urticae, in order to regular replenishment greenhouse Tetranychus urticae propagating population;
D, Phyloseiulus nersimilis isolation breeding: Phyloseiulus nersimilis plant propagation greenhouse independently becomes a greenhouse with clean seedling growing area separately with greenhouse, Tetranychus urticae multiplication plot, and more than 20 meters distances of being separated by, avoid Phyloseiulus nersimilis to import Tetranychus urticae multiplication plot into, affect tetranychid and normally breed;
Wherein:
Greenhouse described in steps A and subregion cooling point four grades, 1. during ambient temperature 25-28 DEG C, only open roof window gravity-flow ventilation; 2., during extraneous 29-32 DEG C, roof window and blower fan is driven; 3., during ambient temperature 33-35 DEG C, wet curtain and blower fan is driven; 4., during ambient temperature more than 36 DEG C, turn on the aircondition; Heat up and adopt heating installation, if when temperature is still lower than 18 DEG C, auxiliary heating of turning on the aircondition;
Microclimate room conservation breeding Tetranychus urticae described in step C, adopts the breeding of bean seedlings inoculation Tetranychus urticae, is cut by partial blade as inoculation carrier inoculation new talent, remainingly bring greenhouse into, supplement its Tetranychus urticae propagating population after 5-7 days;
Run away for preventing Phyloseiulus nersimilis at Phyloseiulus nersimilis isolation multiplication plot (6), breeding of Phyloseiulus nersimilis adopts movable seedbed (2) to place, in rectangle tank (3) central authorities, " one " word is arranged, distance tank dual-side edge is 35cm, water logging in anti-water stopping trough there is not plant, and potted plant heelpiece goes out the water surface; When breeding Phyloseiulus nersimilis more than 40,000 every day, 10-30 mu facilities vegetable tetranychid biological control demand can be met.
2. industrialized propagation Phyloseiulus nersimilis method according to claim 1, it is characterized in that: the greenhouse described in steps A and subregion all have strict seal, greenhouse arranges roof window ventilating opening, 80 order fly nets are all installed on each ventilating opening and both sides, corridor, cooling adopts, and " wet curtain and blower fan " combines and air-conditioning, heats and adopts circular wing type heating installation and air-conditioning.
3. industrialized propagation Phyloseiulus nersimilis method according to claim 1, it is characterized in that: the clean seed described in step B is planted and comprises, potting soil is put into the nutritive cube of medium size 16cm × 14cm, in each nutritive cube, the ground beans of having planted are put on seedbed, are plugged automatic drip irrigation watering facility by plantation 3 ground beans seed in order, a drip irrigation pipe inserted by every basin, water a water every three days, notice that irrigation amount is too much unsuitable, keep soil from packing together; After emerging, watered a chemical fertilizer water every about 10 days, strengthen management, cultivate healthy and strong bean seedlings.
4. industrialized propagation Phyloseiulus nersimilis method according to claim 1, it is characterized in that: the Tetranychus urticae greenhouse mass rearing described in step C is for expanding numerous and inoculation Phyloseiulus nersimilis, method comprises, potted plant for a part fresh healthy and strong bean seedlings are moved into Tetranychus urticae and breeds workshop, every basin inoculation 3-4 sheet is with the blade of tetranychid, on every sheet leaf, the amount of Tetranychus urticae is 50-100 head, when Tetranychus urticae is transferred on new blade, clears up carrier blade in time; Tetranychus urticae greenhouse multiplication plot temperature 18-32 DEG C, humidity 35%-75%.
5. industrialized propagation Phyloseiulus nersimilis method according to claim 1, it is characterized in that: microclimate room surrounding described in step C is that glass curtain wall is beneficial to solar radiation, external door, inside door isolation are set, inner outfit air-conditioning, light compensating lamp, dehumidifier, by automatic controlling system, temperature 20-31 DEG C, humidity 35%-55%.
6. industrialized propagation Phyloseiulus nersimilis method according to claim 1, it is characterized in that: the Phyloseiulus nersimilis isolation breeding described in step D comprises: when the amount of every basin bean seedlings Tetranychus urticae is 800-1000 head, move Phyloseiulus nersimilis plant propagation greenhouse inoculation Predatory Mites to, select the plant leaf containing a large amount of Phyloseiulus nersimilis, cut gently with scissors, be placed in pallet as inoculation carrier, then with tweezers gently gripping blade be placed directly on bean seedlings and inoculate, Phyloseiulus nersimilis can be climbed to very soon on bean seedlings and take food Tetranychus urticae, inoculative proportion is that 1:40-50 is advisable.
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| FR3027488A1 (en) * | 2014-10-23 | 2016-04-29 | Selkys & Co | SYSTEM AND METHOD FOR MASSIVE FARMING OF INSECTS |
| CN106234310B (en) * | 2016-08-01 | 2019-04-09 | 河北省农林科学院旱作农业研究所 | A kind of method of efficient breeding Phyloseiulus nersimilis |
| CN107410217B (en) * | 2017-05-16 | 2023-06-06 | 中国热带农业科学院环境与植物保护研究所 | Breeding device and method for experimental population of cassava single-claw mites |
| CN109699593B (en) * | 2019-03-14 | 2024-02-13 | 山西省农业科学院植物保护研究所 | Fruit tree mite feeding device and use method |
| CN111226652B (en) * | 2020-04-10 | 2024-05-28 | 山东省植物保护总站 | Greenhouse produced by natural enemy insects in matched factory |
| CN112913789B (en) * | 2021-04-02 | 2022-06-03 | 贵州大学 | Predatory mite feeding method taking cattail pollen as feed |
| CN115281158B (en) * | 2022-08-15 | 2023-09-15 | 绵阳市农业科学研究院 | Method for large-scale propagation of phytoseiid chile |
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