CN103499572A - 一种增强型化学发光试剂 - Google Patents
一种增强型化学发光试剂 Download PDFInfo
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- CN103499572A CN103499572A CN201310506254.6A CN201310506254A CN103499572A CN 103499572 A CN103499572 A CN 103499572A CN 201310506254 A CN201310506254 A CN 201310506254A CN 103499572 A CN103499572 A CN 103499572A
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- mmol
- luminol
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- 239000003153 chemical reaction reagent Substances 0.000 title claims abstract description 26
- 238000001378 electrochemiluminescence detection Methods 0.000 title abstract 3
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims abstract description 33
- 238000004020 luminiscence type Methods 0.000 claims abstract description 33
- HWYHZTIRURJOHG-UHFFFAOYSA-N luminol Chemical compound O=C1NNC(=O)C2=C1C(N)=CC=C2 HWYHZTIRURJOHG-UHFFFAOYSA-N 0.000 claims abstract description 33
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 claims abstract description 26
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 claims abstract description 22
- 239000000126 substance Substances 0.000 claims description 30
- 238000013016 damping Methods 0.000 claims description 19
- 239000012530 fluid Substances 0.000 claims description 19
- -1 1-naphthalene boronic acids Chemical class 0.000 claims description 10
- FEDYLAMMQSHIOY-UHFFFAOYSA-N 4-(1,2,4-triazol-1-ylmethyl)aniline;hydrochloride Chemical compound Cl.C1=CC(N)=CC=C1CN1N=CN=C1 FEDYLAMMQSHIOY-UHFFFAOYSA-N 0.000 claims description 8
- UFWIBTONFRDIAS-UHFFFAOYSA-N naphthalene-acid Natural products C1=CC=CC2=CC=CC=C21 UFWIBTONFRDIAS-UHFFFAOYSA-N 0.000 claims description 8
- RVJVDCVIJCBUTH-UHFFFAOYSA-M sodium;5-amino-2h-phthalazin-3-ide-1,4-dione Chemical class [Na+].O=C1N[N-]C(=O)C2=C1C(N)=CC=C2 RVJVDCVIJCBUTH-UHFFFAOYSA-M 0.000 claims description 2
- WHUUTDBJXJRKMK-VKHMYHEASA-N L-glutamic acid Chemical compound OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 claims 4
- 229960002989 glutamic acid Drugs 0.000 claims 2
- 239000000243 solution Substances 0.000 abstract description 23
- WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 abstract description 6
- 235000013922 glutamic acid Nutrition 0.000 abstract description 6
- 239000004220 glutamic acid Substances 0.000 abstract description 6
- 239000007853 buffer solution Substances 0.000 abstract description 3
- MMCPOSDMTGQNKG-UJZMCJRSSA-N aniline;hydrochloride Chemical compound Cl.N[14C]1=[14CH][14CH]=[14CH][14CH]=[14CH]1 MMCPOSDMTGQNKG-UJZMCJRSSA-N 0.000 abstract 1
- 125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 abstract 1
- LZTBZQSQFMLGQH-UHFFFAOYSA-N naphthalen-1-yloxyboronic acid Chemical compound C1=CC=C2C(OB(O)O)=CC=CC2=C1 LZTBZQSQFMLGQH-UHFFFAOYSA-N 0.000 abstract 1
- 239000007788 liquid Substances 0.000 description 12
- 238000003018 immunoassay Methods 0.000 description 8
- 108010001336 Horseradish Peroxidase Proteins 0.000 description 7
- 108090000790 Enzymes Proteins 0.000 description 5
- 102000004190 Enzymes Human genes 0.000 description 5
- 238000005516 engineering process Methods 0.000 description 5
- 239000012744 reinforcing agent Substances 0.000 description 4
- 230000035945 sensitivity Effects 0.000 description 4
- 102000002260 Alkaline Phosphatase Human genes 0.000 description 3
- 108020004774 Alkaline Phosphatase Proteins 0.000 description 3
- 241001597008 Nomeidae Species 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 230000003647 oxidation Effects 0.000 description 3
- 238000007254 oxidation reaction Methods 0.000 description 3
- 238000002360 preparation method Methods 0.000 description 3
- 239000000758 substrate Substances 0.000 description 3
- 238000004458 analytical method Methods 0.000 description 2
- 238000003556 assay Methods 0.000 description 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 239000001301 oxygen Substances 0.000 description 2
- 229910052760 oxygen Inorganic materials 0.000 description 2
- 159000000000 sodium salts Chemical class 0.000 description 2
- 239000002904 solvent Substances 0.000 description 2
- HUDPLKWXRLNSPC-UHFFFAOYSA-N 4-aminophthalhydrazide Chemical compound O=C1NNC(=O)C=2C1=CC(N)=CC=2 HUDPLKWXRLNSPC-UHFFFAOYSA-N 0.000 description 1
- 208000003643 Callosities Diseases 0.000 description 1
- 206010020649 Hyperkeratosis Diseases 0.000 description 1
- 239000000427 antigen Substances 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- 239000000010 aprotic solvent Substances 0.000 description 1
- 239000000872 buffer Substances 0.000 description 1
- 238000006555 catalytic reaction Methods 0.000 description 1
- 238000007385 chemical modification Methods 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000002038 chemiluminescence detection Methods 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 235000014113 dietary fatty acids Nutrition 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 229930195729 fatty acid Natural products 0.000 description 1
- 239000000194 fatty acid Substances 0.000 description 1
- 150000004665 fatty acids Chemical class 0.000 description 1
- 125000000623 heterocyclic group Chemical group 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 230000028993 immune response Effects 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 230000002045 lasting effect Effects 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000005728 strengthening Methods 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
Landscapes
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Description
时间(min) | RLU基础发光液 | RLU增强型化学发光试剂 |
2 | 157 | 47536 |
6 | 178 | 49987 |
10 | 205 | 53624 |
20 | 198 | 47858 |
30 | 185 | 42586 |
50 | 170 | 37859 |
70 | 154 | 33217 |
100 | 139 | 31058 |
150 | 118 | 29854 |
200 | 109 | 24893 |
时间(min) | RLU基础发光液 | RLU增强型化学发光试剂 |
2 | 131 | 26653 |
6 | 159 | 29798 |
10 | 180 | 35462 |
20 | 170 | 31854 |
30 | 159 | 28582 |
50 | 132 | 25754 |
70 | 124 | 22202 |
100 | 114 | 18587 |
150 | 105 | 15854 |
200 | 95 | 12587 |
时间(min) | RLU基础发光液 | RLU增强型化学发光试剂 |
2 | 167 | 57639 |
6 | 185 | 60917 |
10 | 215 | 57658 |
20 | 201 | 50897 |
30 | 195 | 47589 |
50 | 178 | 42639 |
70 | 164 | 38654 |
100 | 142 | 36548 |
150 | 124 | 32857 |
200 | 119 | 28965 |
Claims (4)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
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CN201310506254.6A CN103499572B (zh) | 2013-10-24 | 2013-10-24 | 一种增强型化学发光试剂 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
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CN201310506254.6A CN103499572B (zh) | 2013-10-24 | 2013-10-24 | 一种增强型化学发光试剂 |
Publications (2)
Publication Number | Publication Date |
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CN103499572A true CN103499572A (zh) | 2014-01-08 |
CN103499572B CN103499572B (zh) | 2015-10-28 |
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CN201310506254.6A Active CN103499572B (zh) | 2013-10-24 | 2013-10-24 | 一种增强型化学发光试剂 |
Country Status (1)
Country | Link |
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CN (1) | CN103499572B (zh) |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104629755A (zh) * | 2015-02-02 | 2015-05-20 | 苏州亚科化学试剂股份有限公司 | 复合型有机磷农药残留检测用的鲁米诺类粉末试剂及其制备方法 |
CN109283171A (zh) * | 2017-07-20 | 2019-01-29 | 苏州长光华医生物医学工程有限公司 | 一种化学发光试剂稳定剂的筛选方法 |
CN115128062A (zh) * | 2022-08-29 | 2022-09-30 | 中储粮成都储藏研究院有限公司 | 检测粮食新鲜度的方法及用途 |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1811390A (zh) * | 2006-02-07 | 2006-08-02 | 陕西师范大学 | 纺织物上血迹形态重现的发光成像方法 |
CN102435598A (zh) * | 2011-09-06 | 2012-05-02 | 山东博科生物产业有限公司 | 一种稳定的hrp酶促化学发光底物液 |
-
2013
- 2013-10-24 CN CN201310506254.6A patent/CN103499572B/zh active Active
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1811390A (zh) * | 2006-02-07 | 2006-08-02 | 陕西师范大学 | 纺织物上血迹形态重现的发光成像方法 |
CN102435598A (zh) * | 2011-09-06 | 2012-05-02 | 山东博科生物产业有限公司 | 一种稳定的hrp酶促化学发光底物液 |
Non-Patent Citations (3)
Title |
---|
司梦军: "增强化学发光酶联免疫新体系的研究及应用", 《中国优秀硕士学位论文全文数据库 医药卫生科技辑》, no. 5, 15 May 2009 (2009-05-15), pages 19 - 27 * |
骆建新等: "四苯硼钠和对苯苯酚协同增强化学发光的研究", 《第三军医大学学报》, vol. 24, no. 3, 31 March 2002 (2002-03-31), pages 361 - 363 * |
高新等: "谷氨酸流动注射化学发光法测定", 《理化检验-化学分册》, vol. 40, no. 11, 30 November 2004 (2004-11-30), pages 2 - 1 * |
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104629755A (zh) * | 2015-02-02 | 2015-05-20 | 苏州亚科化学试剂股份有限公司 | 复合型有机磷农药残留检测用的鲁米诺类粉末试剂及其制备方法 |
CN109283171A (zh) * | 2017-07-20 | 2019-01-29 | 苏州长光华医生物医学工程有限公司 | 一种化学发光试剂稳定剂的筛选方法 |
CN109283171B (zh) * | 2017-07-20 | 2020-04-28 | 苏州长光华医生物医学工程有限公司 | 一种化学发光试剂稳定剂的筛选方法 |
CN115128062A (zh) * | 2022-08-29 | 2022-09-30 | 中储粮成都储藏研究院有限公司 | 检测粮食新鲜度的方法及用途 |
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CN103499572B (zh) | 2015-10-28 |
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ASS | Succession or assignment of patent right |
Owner name: SHENZHEN HONGMEI BIOMEDICINE TECHNOLOGY CO., LTD. Free format text: FORMER OWNER: HOU ZHIBO Effective date: 20150806 |
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C41 | Transfer of patent application or patent right or utility model | ||
C53 | Correction of patent of invention or patent application | ||
CB03 | Change of inventor or designer information |
Inventor after: Hou Zhibo Inventor after: Su Ning Inventor before: Hou Zhibo |
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COR | Change of bibliographic data |
Free format text: CORRECT: INVENTOR; FROM: HOU ZHIBO TO: HOU ZHIBO SU NING |
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TA01 | Transfer of patent application right |
Effective date of registration: 20150806 Address after: 518000, a bio incubator building in central high tech Zone, Shenzhen, Guangdong 1 - 104, Nanshan District Applicant after: SHENZHEN HONGMEI BIOPHARMACEUTICAL TECHNOLOGY Co.,Ltd. Address before: 300000 Tianjin City, Nankai District Wei Jin Road No. 94 Applicant before: Hou Zhibo |
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C53 | Correction of patent of invention or patent application | ||
CB03 | Change of inventor or designer information |
Inventor after: Hou Zhibo Inventor after: Xu Jiake Inventor after: Zheng Jingyuan Inventor after: Su Yu Inventor after: Zhang Wei Inventor after: Fang Yanxiong Inventor after: Du Zhiyun Inventor before: Hou Zhibo Inventor before: Su Ning |
|
COR | Change of bibliographic data |
Free format text: CORRECT: INVENTOR; FROM: HOU ZHIBO SU NING TO: HOU ZHIBO XU JIAKE ZHENG JINGYUAN SU YU ZHANG WEI FANG YANXIONG DU ZHIYUN |
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C14 | Grant of patent or utility model | ||
GR01 | Patent grant | ||
TR01 | Transfer of patent right | ||
TR01 | Transfer of patent right |
Effective date of registration: 20191028 Address after: 518000 Guangdong province Shenzhen City Dapeng new Kwai Street Jinling Road No. 1 International Bio Valley Life Science Park A22 building 3, 4 floor Patentee after: Shenzhen Hongmei Diagnostic Technology Co., Ltd. Address before: 518000 1-104 biological incubator building, No.1 high tech Middle Road, high tech Zone, Nanshan District, Shenzhen City, Guangdong Province Patentee before: SHENZHEN HONGMEI BIOPHARMACEUTICAL TECHNOLOGY Co.,Ltd. |