CN103497838B - Method for extracting peony essential oil from peonies - Google Patents
Method for extracting peony essential oil from peonies Download PDFInfo
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- CN103497838B CN103497838B CN201310506233.4A CN201310506233A CN103497838B CN 103497838 B CN103497838 B CN 103497838B CN 201310506233 A CN201310506233 A CN 201310506233A CN 103497838 B CN103497838 B CN 103497838B
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Abstract
The invention relates to a method for extracting peony essential oil from peonies. The method comprises the following steps that step 1, the peonies are picked, forced air drying is conducted on the peonies, and the dried peonies are taken out and ground; step 2, a cellulase water solution and a pectinase water solution are mixed, acetum is added, and therefore a phosphoesterases complex solution is obtained; step 3, the ground peonies are placed in a triangular flask, the phosphoesterases complex solution is added, standing is conducted, ultrasonic treatment is conducted, and standing is conducted; step 4, the obtained solution in the step 3 is added to a petroleum ether ethanol solution, backflow is conducted, digestion is conducted at the indoor temperature, and the obtained solution is transferred into a rotary evaporator for vacuum concentration. According to the method, enzyme treatment is firstly conducted on the peonies so that local changes of loosening, expansion, crashing and the like are generated in the cell walls of the peonies and the mesenchyme structures of the cell walls; then, ultrasonic treatment is conducted on the peonies for a short time, and therefore the extraction rate of the essential oil from the cells of the peonies is greatly improved.
Description
Technical field
The invention belongs to plant biotechnology field, be specifically related to a kind of method extracting peony essential oil from peony.
Background technology
Peony essential oil extracts plant essence from the flower of tree peony, leaf, root, fruit, is rich in amino acid and trace element that multiple human body needs.It effectively can suppress skin pigment formation, smoothing wrinkle, minimizing skin splash and the pregnant occasion line of women by scavenging activated oxygen.Improve blood microcirculation, remove skin toxins, improve dry skin and pore refining, make skin smooth, flexible.Peony essential oil is natural plant hormone, and hormone secretion in balance women body, alleviates menalgia, nourish uterus.Anti-oxidant: distinctive tree peony polyphenol in peony essential oil is very strong antioxidant, can also extend other antioxidants time in vivo, delay skin aging.Suppress color spot, senile plaque expelling simultaneously, prevent and treat acne, bright and clean skin quality.Therefore a kind of method extracting peony essential oil fast and efficiently from peony is studied significant.
The extracting method of current peony essential oil has four kinds of methods, steam distillation, and the active ingredient of peony essential oil is damaged, and causes the yield of peony essential oil and quality to decline.Organic solvent extraction needs the time long, the waste extensive work time.The operating process of adsorbing and extracting method is many, and the production cycle is long, production efficiency is low.Supercritical CO_2 extraction method has to technological process that strict demand, required condition are high, apparatus expensive.
Summary of the invention
The object of the present invention is to provide a kind of method extracting peony essential oil from peony, peony essential oil can be extracted fast and efficiently from peony.
The technical solution used in the present invention is, a kind of method extracting peony essential oil from peony, comprises the following steps:
Step one, harvesting ripe, disease-free peony, be placed in the baking oven forced air drying that temperature is 25 ~ 30 DEG C by peony, take out peony afterwards and be crushed to 40 orders, for subsequent use;
The pectase aqueous solution equal-volume mixing of step 2, to be the cellulase aqueous solution of 0.2mg/mL and mass concentration by mass concentration be 0.1mg/mL, the acetum that mass percent concentration is 2% is added in mixed liquor, until mixed liquor pH value reaches 5.0, leave standstill 10 ~ 15min after being stirred by mixed liquor, obtain complex enzyme liquid;
Step 3, peony step one obtained put into triangular flask, complex enzyme liquid prepared by step 2 is added in triangular flask, the ratio of complex enzyme liquid and peony addition is 45:1, wherein complex enzyme liquid is in milliliter, peony in gram, first the triangular flask with peony being placed into temperature is afterwards leave standstill 20min under the condition of 35 DEG C, then this temperature is kept, and the ultrasonic wave process 5min that frequency is 22 kHz is applied to triangular flask, finally triangular flask being transferred to temperature is leave standstill 40min under the condition of 40 DEG C, for subsequent use;
Step 4, in the triangular flask after leaving standstill, to add mass concentration be refluxing extraction 1h in the benzinum ethanolic solution of 2 ~ 3kg/L, benzinum ethanolic solution addition is 5 times of solution in triangular flask, lixiviate 1h under room temperature, proceeding to temperature is afterwards reduced pressure concentration in the Rotary Evaporators of 40 ~ 45 DEG C, and being concentrated into temperature is that under the condition of 40 ~ 45 DEG C, relative density is 1.0g/mL.
Beneficial effect of the present invention has the following aspects:
One, the present invention first under temperature is the condition of 35 DEG C to peony ferment treatment 20min, peony cell membrane and interstitial structure thereof can be made to produce the change such as loose, expansion, collapse of local; Afterwards peony is applied to the ultrasonic wave process of short time, ultrasonic wave produces strong shock wave and microjet in the inside of liquid, this powerful impingement flow can effectively reduce, eliminate block layer between solvent and aqueous phase, thus increase mass transfer rate, in addition, impingement flow produces a kind of physical shear power to cell tissue, make it distortion, break, and discharge inclusion, cell membrane and interstitial structure thereof produce local loose, expand, extraction process that this accelerates peony essential oil in cell greatly, serve the effect of getting twice the result with half the effort; After ultrasonic wave process, peony being transferred to temperature is continue enzymolysis 40min under the condition of 40 DEG C, cell membrane and interstitial thereof can be made to entirely collapse, substantially increase the recovery rate of peony essential oil in peony cell.
Two, the ultrasonic frequency in the present invention is only 22kHz, the activated centre of cellulase and pectase can be made to be moved, hydrophobicity is strengthened, promote better to be combined with substrate during enzyme digestion reaction, promote that peony cell membrane and interstitial structure thereof are collapsed faster, intracellular peony essential oil is entered in solvent faster.
Three, the present invention is by destroying cell membrane and interstitial structure thereof, peony essential oil can be made to enter into solvent fast, substantially reduce extraction time, also improve the recovery rate of peony essential oil simultaneously.
Four, the present invention is extracted as auxiliary method by taking to be extracted as main, ultrasonic wave with enzyme, avoid use ultrasonic wave extract sample size large time, the ultrasonic energy decay arriving sample interior is serious, the problem of extraction effect difference.
Detailed description of the invention
Elaborate to embodiments of the invention below, the present embodiment is implemented under premised on technical solution of the present invention, give detailed embodiment and concrete operating process, but protection scope of the present invention is not limited to following embodiment.
From peony, extract a method for peony essential oil, comprise the following steps:
Step one, harvesting ripe, disease-free peony, be placed in the baking oven forced air drying that temperature is 25 ~ 30 DEG C by peony, take out peony afterwards and be crushed to 40 orders, for subsequent use;
The pectase aqueous solution equal-volume mixing of step 2, to be the cellulase aqueous solution of 0.2mg/mL and mass concentration by mass concentration be 0.1mg/mL, the acetum that mass percent concentration is 2% is added in mixed liquor, until mixed liquor pH value reaches 5.0, leave standstill 10 ~ 15min after being stirred by mixed liquor, obtain complex enzyme liquid;
Step 3, peony step one obtained put into triangular flask, complex enzyme liquid prepared by step 2 is added in triangular flask, the ratio of complex enzyme liquid and peony addition is 45:1, wherein complex enzyme liquid is in milliliter, peony in gram, first the triangular flask with peony being placed into temperature is afterwards leave standstill 20min under the condition of 35 DEG C, then this temperature is kept, and the ultrasonic wave process 5min that frequency is 22 kHz is applied to triangular flask, finally triangular flask being transferred to temperature is leave standstill 40min under the condition of 40 DEG C, for subsequent use;
Step 4, in the triangular flask after leaving standstill, to add mass concentration be refluxing extraction 1h in the benzinum ethanolic solution of 2 ~ 3kg/L, benzinum ethanolic solution addition is 5 times of solution in triangular flask, lixiviate 1h under room temperature, proceeding to temperature is afterwards reduced pressure concentration in the Rotary Evaporators of 40 ~ 45 DEG C, and being concentrated into temperature is that under the condition of 40 ~ 45 DEG C, relative density is 1.0g/mL.
Embodiment 1
From peony, extract a method for peony essential oil, comprise the following steps:
Step one, harvesting ripe, disease-free peony, be placed in the baking oven forced air drying that temperature is 30 DEG C by peony, take out peony afterwards and be crushed to 40 orders, for subsequent use;
The pectase aqueous solution equal-volume mixing of step 2, to be the cellulase aqueous solution of 0.2mg/mL and mass concentration by mass concentration be 0.1mg/mL, the acetum that mass percent concentration is 2% is added in mixed liquor, until mixed liquor pH value reaches 5.0, leave standstill 12min after being stirred by mixed liquor, obtain complex enzyme liquid;
Step 3, peony step one obtained put into triangular flask, complex enzyme liquid prepared by step 2 is added in triangular flask, the ratio of complex enzyme liquid and peony addition is 45:1, wherein complex enzyme liquid is in milliliter, peony in gram, first the triangular flask with peony being placed into temperature is afterwards leave standstill 20min under the condition of 35 DEG C, then this temperature is kept, and the ultrasonic wave process 5min that frequency is 22 kHz is applied to triangular flask, finally triangular flask being transferred to temperature is leave standstill 40min under the condition of 40 DEG C, for subsequent use;
Step 4, in the triangular flask after leaving standstill, to add mass concentration be refluxing extraction 1h in the benzinum ethanolic solution of 2.5kg/L, benzinum ethanolic solution addition is 5 times of solution in triangular flask, lixiviate 1h under room temperature, proceeding to temperature is afterwards reduced pressure concentration in the Rotary Evaporators of 42 DEG C, and being concentrated into temperature is that under the condition of 42 DEG C, relative density is 1.0g/mL.
Experimental result shows: the present invention is compared with conventional organic solvent extraction, and extraction time shortens one hour, and recovery rate increases 13%, achieves outstanding significant progressive.
Claims (1)
1. from peony, extract a method for peony essential oil, it is characterized in that, comprise the following steps:
Step one, harvesting ripe, disease-free peony, be placed in the baking oven forced air drying that temperature is 25 ~ 30 DEG C by peony, take out peony afterwards and be crushed to 40 orders, for subsequent use;
The pectase aqueous solution equal-volume mixing of step 2, to be the cellulase aqueous solution of 0.2mg/mL and mass concentration by mass concentration be 0.1mg/mL, the acetum that mass percent concentration is 2% is added in mixed liquor, until mixed liquor pH value reaches 5.0, leave standstill 10 ~ 15min after being stirred by mixed liquor, obtain complex enzyme liquid;
Step 3, peony step one obtained put into triangular flask, complex enzyme liquid prepared by step 2 is added in triangular flask, the ratio of complex enzyme liquid and peony addition is 45:1, wherein complex enzyme liquid is in milliliter, peony in gram, first the triangular flask with peony being placed into temperature is afterwards leave standstill 20min under the condition of 35 DEG C, then this temperature is kept, and the ultrasonic wave process 5min that frequency is 22 kHz is applied to triangular flask, finally triangular flask being transferred to temperature is leave standstill 40min under the condition of 40 DEG C, for subsequent use;
Step 4, in the triangular flask after leaving standstill, to add mass concentration be refluxing extraction 1h in the benzinum ethanolic solution of 2 ~ 3kg/L, benzinum ethanolic solution addition is 5 times of solution in triangular flask, lixiviate 1h under room temperature, proceeding to temperature is afterwards reduced pressure concentration in the Rotary Evaporators of 40 ~ 45 DEG C, and being concentrated into temperature is that under the condition of 40 ~ 45 DEG C, relative density is 1.0g/mL.
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Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103784523A (en) * | 2014-01-28 | 2014-05-14 | 齐鲁工业大学 | Peony polyphenol extraction process |
| CN104277915A (en) * | 2014-09-25 | 2015-01-14 | 齐鲁工业大学 | Method for extracting peony essential oil from peony |
| CN107308100B (en) * | 2017-07-21 | 2020-07-07 | 江苏岩藤农业发展有限公司 | Additive for preventing peony essential oil from volatilizing, preparation method thereof and peony essential oil containing additive |
| CN109730948A (en) * | 2019-01-24 | 2019-05-10 | 山东贝世康生物科技有限公司 | The method and application of fresh peony flower cellular water are extracted from fresh peony flower |
| CN109588722B (en) * | 2019-02-13 | 2022-10-14 | 山东贝世康生物科技有限公司 | Compound peony seed oil soft capsule with blood pressure lowering effect and preparation method thereof |
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| KR100986894B1 (en) * | 2008-08-21 | 2010-10-08 | (주)아모레퍼시픽 | Perfume composition for expressing the fragrance of Paeonia suffruticosa |
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| CN101213994A (en) * | 2008-01-03 | 2008-07-09 | 陕西师范大学 | Method for using ultrasound wave auxiliary enzyme to extract apple seed oil |
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