CN103496793A - Method for fast removing azo dyes from sewage in aerobic conditions - Google Patents
Method for fast removing azo dyes from sewage in aerobic conditions Download PDFInfo
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- CN103496793A CN103496793A CN201310491252.4A CN201310491252A CN103496793A CN 103496793 A CN103496793 A CN 103496793A CN 201310491252 A CN201310491252 A CN 201310491252A CN 103496793 A CN103496793 A CN 103496793A
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- Prior art keywords
- sewage
- azo dyes
- pseudomonas aeruginosa
- azoic dyestuff
- aerobic conditions
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W10/00—Technologies for wastewater treatment
- Y02W10/10—Biological treatment of water, waste water, or sewage
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
Abstract
The invention discloses a method for fast removing azo dyes from sewage in aerobic conditions by the aid of microorganisms. The method includes: cultivating pseudomonas aeruginosa for some periods, removing supernatant through centrifugation so as to enable the pseudomonas aeruginosa to heavily suspend in neutral PBS; adding a certain amount of the aze dyes into bacteria suspension liquid and cultivating the bacteria suspension liquid on a constant-temperature shaking table in a shaking manner in the aerobic condition to achieve the purpose of removing the azo dyes from the sewage. Aiming to overcome the defect that a method for removing the azo dyes by the aid of ordinary microorganisms is in need of keeping anaerobic condition and is low in speed, the method for fast removing the azo dyes from the sewage in the aerobic conditions can be operated under non-anaerobic conditions, and is high in processing speed.
Description
Technical field
The present invention relates to the method for azoic dyestuff in a kind of quick removal sewage, utilize the characteristics of microbial environment protecting economy, belong to water and pollute the reparation field.
Background technology
Along with the development of chemical industry, various dyestuffs are widely applied in the industries such as weaving, makeup, printing, have caused a large amount of discharges of coloured sewage.The coloured sewage be discharged in environment not only causes chroma pollution that the water body transmittance is reduced, and causes the deterioration of water ecological setting; And dye well degradation production wherein has bio-toxicity, carcinogenic, teratogenesis, mutagenic effect are arranged.Therefore in waste water from dyestuff, the removal of dyestuff gets the attention.
The maximum dyestuff of application is azoic dyestuff at present, accounts for 70% of total amount.Azoic dyestuff is various in style, complex structure, chemical stability is good, biodegradability is poor.In treating processes, physico-chemical process and the method for being combined with biotechnology thereof can effectively be degraded by azo dyes, but cost compare is high, easily produce secondary pollution.Biological degradation method is effective means the most at present, and treatment effect is good and cost is low.Yet separate at present the Degradation of Azo Dyes bacterium obtained and generally all need under anaerobic could realize degraded, and treatment rate is slow.Therefore, obtaining some can be the important research direction of processing azo dye wastewater at the bacterial strain of aerobic lower efficient degradation.
Summary of the invention
Technical scheme:
The purpose of this invention is to provide a kind of microorganism quick method of removing azoic dyestuff in sewage under aerobic condition of utilizing.
Concrete application method:
(1) the bacterium liquid centrifugal (6000 r/min, 5 min) that will be cultured to logarithmic phase removes supernatant, with after the sterile distilled water washing, thalline is resuspended in to PBS(50 mM, pH=7.0) in.(2) gained bacterium liquid in 1 is placed in to Erlenmeyer flask, splashes into a certain amount of azoic dyestuff (12.5 mg/L).(3) Erlenmeyer flask in 2 is placed in to the aerobic cultivation of constant-temperature table concussion, can realizes the removal of azo dyes.
Beneficial effect of the present invention is: the method for azoic dyestuff in the removal sewage provided, simple to operation, treatment effect is fast, has the possibility in the actual dye wastewater of being applied to processing.
The accompanying drawing explanation
Fig. 1: the tropeolin-D clearance over time.
Fig. 2: tropeolin-D is processed the front and back spectrogram.
Fig. 3: the Viola crystallina clearance over time.
Fig. 4: Viola crystallina is processed the front and back spectrogram.
Embodiment
Embodiment 1: after Pseudomonas aeruginosa is cultured to logarithmic phase, centrifugal (6000 r/min, 5 min) remove supernatant, are resuspended to PBS(50 mM, pH=7.0) in.Resuspension gained bacterium liquid 40 mL are added in 100 mL Erlenmeyer flasks, and splash into the aqueous solution of 1 mL containing 0.5 g/L tropeolin-D, the final concentration that makes tropeolin-D in solution is 12.5 mg/L.Erlenmeyer flask is placed in to constant temperature (37 ℃) shaking table concussion (130 r/min) aerobic cultivation, and sampling detects clearance.Wherein, clearance=(original liquid concentration-retained concentration)/original liquid concentration.Fig. 1 shows the clearance of tropeolin-D, and in 3 h, clearance reaches 86.0%.Fig. 2 shows the spectrogram after tropeolin-D is processed front and processing 18 h.Illustrate that Pseudomonas aeruginosa has played good removal effect to tropeolin-D.
Embodiment 2: after Pseudomonas aeruginosa is cultured to logarithmic phase, centrifugal (6000 r/min, 5 min) remove supernatant, are resuspended to PBS(50 mM, pH=7.0) in.Resuspension gained bacterium liquid 40mL is added in 100 mL Erlenmeyer flasks, and splash into the aqueous solution of 1 mL containing 0.5 g/L Viola crystallina, the final concentration that makes Viola crystallina in solution is 12.5 mg/L.Erlenmeyer flask is placed in to constant temperature (37 ℃) shaking table concussion (130 r/min) aerobic cultivation, and sampling detects clearance.Wherein, clearance=(original liquid concentration-retained concentration)/original liquid concentration.Fig. 3 shows the clearance of Viola crystallina, and in 3 h, clearance reaches 66.1%.Fig. 2 shows the spectrogram after Viola crystallina is processed front and processing 18 h.Illustrate that Pseudomonas aeruginosa has played good removal effect to Viola crystallina.
Claims (2)
1. a method of utilizing microorganism to remove azoic dyestuff in sewage is characterized in that: the microorganism Pseudomonas aeruginosa that utilizes can under aerobic condition, remove fast the azoic dyestuff in sewage.
2. a method of utilizing microorganism to remove azoic dyestuff in sewage is characterized in that: by following detailed process, realize:
(1) will be cultured to the centrifugal supernatant that goes of Pseudomonas aeruginosa of logarithmic phase, clean with sterile distilled water, thalline will be resuspended in aseptic neutral PBS; (2) gained bacterium liquid in 1 is placed in to Erlenmeyer flask, after splashing into a certain amount of azoic dyestuff, Erlenmeyer flask is placed in to the aerobic cultivation of constant-temperature table concussion, can realize the removal of azo dyes.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310491252.4A CN103496793A (en) | 2013-10-21 | 2013-10-21 | Method for fast removing azo dyes from sewage in aerobic conditions |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310491252.4A CN103496793A (en) | 2013-10-21 | 2013-10-21 | Method for fast removing azo dyes from sewage in aerobic conditions |
Publications (1)
| Publication Number | Publication Date |
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| CN103496793A true CN103496793A (en) | 2014-01-08 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201310491252.4A Pending CN103496793A (en) | 2013-10-21 | 2013-10-21 | Method for fast removing azo dyes from sewage in aerobic conditions |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101134954A (en) * | 2007-07-30 | 2008-03-05 | 江苏省淡水水产研究所 | Immobilized biological agent and method for degrading bice green contamination of cultivation aquifer |
| CN102167448A (en) * | 2010-12-03 | 2011-08-31 | 中国地质大学（武汉） | Application of aniline degradation strain NO.3 |
| CN103146621A (en) * | 2013-03-21 | 2013-06-12 | 青岛蔚蓝生物集团有限公司 | Pseudomonas aeruginosa and application thereof |
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2013
- 2013-10-21 CN CN201310491252.4A patent/CN103496793A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101134954A (en) * | 2007-07-30 | 2008-03-05 | 江苏省淡水水产研究所 | Immobilized biological agent and method for degrading bice green contamination of cultivation aquifer |
| CN102167448A (en) * | 2010-12-03 | 2011-08-31 | 中国地质大学（武汉） | Application of aniline degradation strain NO.3 |
| CN103146621A (en) * | 2013-03-21 | 2013-06-12 | 青岛蔚蓝生物集团有限公司 | Pseudomonas aeruginosa and application thereof |
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Application publication date: 20140108 |