CN103493733A - Method for quickly inducing generation of fritillaria cirrhosa bulb - Google Patents
Method for quickly inducing generation of fritillaria cirrhosa bulb Download PDFInfo
- Publication number
- CN103493733A CN103493733A CN201310437399.5A CN201310437399A CN103493733A CN 103493733 A CN103493733 A CN 103493733A CN 201310437399 A CN201310437399 A CN 201310437399A CN 103493733 A CN103493733 A CN 103493733A
- Authority
- CN
- China
- Prior art keywords
- bulb
- culture
- condition
- bulbus fritillariae
- fritillariae cirrhosae
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention discloses a method for quickly inducing generation of fritillaria cirrhosa bulb, belonging to the field of biotechnology. In the method provided by the invention, a tissue culture bulb is selected to be put in MS+KT1-3mg.L-1+NAA0.1-1mg.L-1 culture medium, after the tissue culture bulb is cultured for 30-40 days at the culture temperature of 12-18 DEG C without illumination, succulent juvenile leaves of fritillaria cirrhosa generated by the tissue culture bulb are obtained, then, the succulent juvenile leaves of fritillaria cirrhosa are put in a bulb generation inducing culture medium for cultivation, thus, regeneration of fritillaria cirrhosa bulb on the succulent juvenile leaves of fritillaria cirrhosa is realized, and a new way for quick and massive generation of medicinal parts of fritillaria cirrhosa is provided. The method has simple steps and strong operability, has the characteristics of low investment, low cost and high efficiency, and has important practical significance for solving the problem of shortage of fritillaria cirrhosa medicinal materials.
Description
Technical field
The invention belongs to biological technical field, relate to particularly a kind of method that rapid induction Bulbus Fritillariae Cirrhosae bulb produces.
Background technology
Bulbus Fritillariae Cirrhosae (Fritillaria cirrhosa D.Don) is the Liliaceae herbaceos perennial, with bulb, is used as medicine, and be famous and precious Genuine crude drugs in Sichuan, can be rated as the treasured in medicine.The Bulbus Fritillariae Cirrhosae plant requires harsh to growing environment, it like cold cloudy weather conditions, have cold-resistant, happiness is wet, happiness covers, the characteristic of fearness high temperature.Temperature reaches 30 ℃ or ground temperature and surpasses 25 ℃, and plant will wither, and can not survive in the area low at height above sea level, that temperature is high, so Bulbus Fritillariae Cirrhosae could normal growth at the height of height above sea level 3500m～4500m.Bulbus Fritillariae Cirrhosae is the good medicine that important treatment is coughed, for lung-heat type cough, and the few phlegm of dry cough, deficiency of Yin labor is coughed, and coughs up the symptoms such as sputum streaked with blood.Because Bulbus Fritillariae Cirrhosae is expensive, in recent years without planning excavating of blindness, natural resources is increasingly exhausted, estimates also be difficult to alleviation within nearly decades in addition.The tissue culture technique growth cycle is short, reproduction rate is high, broken away from the adverse effect of the four seasons, variation round the clock and disastrous weather in the Nature, and condition homogeneous, very favourable to plant growth, be convenient to stably carry out cultivate in the anniversary and produce, it is one of effective ways that solve Bulbus Fritillariae Cirrhosae herb resource shortage problem that the method that therefore the application tissue is cultivated is carried out the Bulbus Fritillariae Cirrhosae Fast-propagation.The generation that existing employing Bulbus Fritillariae Cirrhosae Plant Leaf is the explant induction bulb all will produce the acquisition bulb again through the callus cultivation stage.As everyone knows, organize culture materials in the process callus chromosomal variation that during stage, cell more easily produces, thus the genetic stability that is unfavorable for guaranteeing to cultivate the bulb medicinal material.
Summary of the invention
The object of the invention is to overcome the deficiencies in the prior art, a kind of method that provides rapid induction Bulbus Fritillariae Cirrhosae bulb to produce, the method step is simple, workable, and process stabilizing is applicable to the extensive use in industrialized production.
The objective of the invention is to be achieved through the following technical solutions:
A kind of method that rapid induction Bulbus Fritillariae Cirrhosae bulb produces, it comprises the following steps:
S1: choose the diameter of inducing generation and train bulb in the group of 0.5～2cm, access MS+KT 1～3mgL
-1+ NAA 0.1～1 mgL
-1in medium, cultivate, condition of culture is: cultivation temperature is 12～18 ℃ cultivates 30～40 days according under condition with unglazed, obtains the Bulbus Fritillariae Cirrhosae carnification spire produced by group training bulb;
S2: the carnification spire that to choose the spire upper end be white or yellow-white, from leaf sheath base cuts, access the medium MS+6-BA 1～3mgL that induces bulb to produce by it in the mode kept flat
-1+ TDZ 0.1～0.5mgL
-1+ IAA 0.1～1mgL
-1+ Ad 10～25mgL
-1in cultivated, condition of culture is: it is to cultivate 5～10 days under the condition of culture of 4～8 ℃ and unglazed photograph that culture materials is placed on;
S3: it is 15～28 ℃ that the culture materials after the S2 step process is placed on to cultivation temperature, and illumination every day 4～8h cultivates under the condition of culture that intensity of illumination is 200～600lx 20～40 days.
Preferably, the group training bulb described in the S1 step is directly 1.2cm, access MS+KT 2mgL
-1+ NAA 0.5mgL
-1medium in.
Preferably, the cultivation temperature described in the S1 step is 15 ℃.
Preferably, the condition of culture described in the S1 step is unglazed according to cultivating 35 days under condition.
Preferably, the medium of inducing bulb to produce described in the S2 step is MS+6-BA 2mgL
-1+ TDZ 0.3mgL
-1+ IAA 0.5mgL
-1+ Ad 18mgL
-1.
Preferably, the cultivation temperature described in the S2 step is: 6 ℃, and cultivate 7 days under the condition of culture of unglazed photograph.
Preferably, the cultivation temperature described in the S3 step is: 20 ℃, every day, light application time was 6h, under the condition of culture that intensity of illumination is 400lx, cultivated 30 days.
Beneficial effect of the present invention is:
The present invention has realized Direct Regeneration Bulbus Fritillariae Cirrhosae bulb from Bulbus Fritillariae Cirrhosae meat spire, for the rapid batch of Bulbus Fritillariae Cirrhosae medicinal part produces, provides a new way.The method can significantly improve the sprout rate of bulb, obviously shortens the output that improves Bulbus Fritillariae Cirrhosae crop cycle.The method step is simple, workable, and process stabilizing is applicable to the extensive use in industrialized production.
Embodiment
Below in conjunction with embodiment, the present invention will be further described, and protection scope of the present invention is not limited to the following stated:
Embodiment 1: a kind of method that rapid induction Bulbus Fritillariae Cirrhosae bulb produces, and it comprises the following steps:
S1: choose the diameter of inducing generation and train bulb in the group of 0.5～2cm, access MS+KT 1～3mgL
-1+ NAA 0.1～1mgL
-1in medium, cultivate, condition of culture is: cultivation temperature is 12～18 ℃ cultivates 30～40 days according under condition with unglazed, obtains the Bulbus Fritillariae Cirrhosae carnification spire produced by group training bulb;
S2: the carnification spire that to choose the spire upper end be white or yellow-white, from leaf sheath base cuts, access the medium MS+6-BA 1～3mgL that induces bulb to produce by it in the mode kept flat
-1+ TDZ 0.1～0.5mgL
-1+ IAA 0.1～1mgL
-1+ Ad 10～25mgL
-1in cultivated, condition of culture is: it is to cultivate 5～10 days under the condition of culture of 4～8 ℃ and unglazed photograph that culture materials is placed on;
S3: it is 15～28 ℃ that the culture materials after the S2 step process is placed on to cultivation temperature, and illumination every day 4～8h cultivates under the condition of culture that intensity of illumination is 200～600lx 20～40 days.
Embodiment 2: a kind of method that rapid induction Bulbus Fritillariae Cirrhosae bulb produces, and it comprises the following steps:
S1: choose the diameter of inducing generation and train bulb in the group of 1.2cm, access MS+KT 2mgL
-1+ NAA 0.5mgL
-1in medium, cultivate, condition of culture is: cultivation temperature is 15 ℃ and unglazed according to cultivating 35 days under condition, obtains the Bulbus Fritillariae Cirrhosae carnification spire produced by group training bulb;
S2: the carnification spire that to choose the spire upper end be white or yellow-white, from leaf sheath base cuts, access the medium MS+6-BA 2mgL that induces bulb to produce by it in the mode kept flat
-1+ TDZ 0.3mgL
-1+ IAA 0.5mgL
-1+ Ad 18mgL
-1in cultivated, condition of culture is: it is to cultivate 7 days under the condition of culture of 6 ℃ and unglazed photograph that culture materials is placed on;
S3: it is 20 ℃ that the culture materials after the S2 step process is placed on to cultivation temperature, and illumination every day 6h cultivates 30 days under the condition of culture that intensity of illumination is 400lx.
Embodiment 3: a kind of method that rapid induction Bulbus Fritillariae Cirrhosae bulb produces, and it comprises the following steps:
S1: choose the diameter of inducing generation and train bulb in the group of 0.5cm, access MS+KT 1mgL
-1+ NAA 0.1mgL
-1in medium, cultivate, condition of culture is: cultivation temperature is 12 ℃ and unglazed according to cultivating 30 days under condition, obtains the Bulbus Fritillariae Cirrhosae carnification spire produced by group training bulb;
S2: the carnification spire that to choose the spire upper end be white or yellow-white, from leaf sheath base cuts, access the medium MS+6-BA 1mgL that induces bulb to produce by it in the mode kept flat
-1+ TDZ 0.1mgL
-1+ IAA 0.1mgL
-1+ Ad 10mgL
-1in cultivated, condition of culture is: it is to cultivate 5 days under the condition of culture of 4 ℃ and unglazed photograph that culture materials is placed on;
S3: it is 15 ℃ that the culture materials after the S2 step process is placed on to cultivation temperature, and illumination every day 4h cultivates 20 days under the condition of culture that intensity of illumination is 200lx.
Embodiment 4: a kind of method that rapid induction Bulbus Fritillariae Cirrhosae bulb produces, and it comprises the following steps:
S1: choose the diameter of inducing generation and train bulb in the group of 2cm, access MS+KT 3mgL
-1+ NAA 1mgL
-1in medium, cultivate, condition of culture is: cultivation temperature is 18 ℃ and unglazed according to cultivating 40 days under condition, obtains the Bulbus Fritillariae Cirrhosae carnification spire produced by group training bulb;
S2: the carnification spire that to choose the spire upper end be white or yellow-white, from leaf sheath base cuts, access the medium MS+6-BA 3mgL that induces bulb to produce by it in the mode kept flat
-1+ TDZ 0.5mgL
-1+ IAA 1mgL
-1+ Ad 25mgL
-1in cultivated, condition of culture is: it is to cultivate 10 days under the condition of culture of 8 ℃ and unglazed photograph that culture materials is placed on;
S3: it is 28 ℃ that the culture materials after the S2 step process is placed on to cultivation temperature, and illumination every day 8h cultivates 40 days under the condition of culture that intensity of illumination is 600lx.
Embodiment 5: a kind of method that rapid induction Bulbus Fritillariae Cirrhosae bulb produces, and it comprises the following steps:
S1: choose the diameter of inducing generation and train bulb in the group of 1cm, access MS+KT 2mgL
-1+ NAA 1mgL
-1in medium, cultivate, condition of culture is: cultivation temperature is 15 ℃ and unglazed according to cultivating 35 days under condition, obtains the Bulbus Fritillariae Cirrhosae carnification spire produced by group training bulb;
S2: the carnification spire that to choose the spire upper end be white or yellow-white, from leaf sheath base cuts, access the medium MS+6-BA 2mgL that induces bulb to produce by it in the mode kept flat
-1+ TDZ 0.3mgL
-1+ IAA 0.5mgL
-1+ Ad 18mgL
-1in cultivated, condition of culture is: it is to cultivate 7 days under the condition of culture of 6 ℃ and unglazed photograph that culture materials is placed on;
S3: it is 18 ℃ that the culture materials after the S2 step process is placed on to cultivation temperature, and illumination every day 6h cultivates 35 days under the condition of culture that intensity of illumination is 400lx.
Embodiment 6: a kind of method that rapid induction Bulbus Fritillariae Cirrhosae bulb produces, and it comprises the following steps:
S1: choose the diameter of inducing generation and train bulb in the group of 0.6cm, access MS+KT 1.5mgL
-1+ NAA 0.6mgL
-1in medium, cultivate, condition of culture is: cultivation temperature is 15 ℃ and unglazed according to cultivating 30 days under condition, obtains the Bulbus Fritillariae Cirrhosae carnification spire produced by group training bulb;
S2: the carnification spire that to choose the spire upper end be white or yellow-white, from leaf sheath base cuts, access the medium MS+6-BA 1.5mgL that induces bulb to produce by it in the mode kept flat
-1+ TDZ 0.5mgL
-1+ IAA 0.6mgL
-1+ Ad 18mgL
-1in cultivated, condition of culture is: it is to cultivate 8 days under the condition of culture of 7 ℃ and unglazed photograph that culture materials is placed on;
S3: it is 18 ℃ that the culture materials after the S2 step process is placed on to cultivation temperature, and illumination every day 8h cultivates 20 days under the condition of culture that intensity of illumination is 300lx.
Embodiment 7: a kind of method that rapid induction Bulbus Fritillariae Cirrhosae bulb produces, and it comprises the following steps:
S1: choose the diameter of inducing generation and train bulb in the group of 1.5cm, access MS+KT 2mgL
-1+ NAA 0.5 mgL
-1in medium, cultivate, condition of culture is: cultivation temperature is 16 ℃ and unglazed according to cultivating 35 days under condition, obtains the Bulbus Fritillariae Cirrhosae carnification spire produced by group training bulb;
S2: the carnification spire that to choose the spire upper end be white or yellow-white, from leaf sheath base cuts, access the medium MS+6-BA 2mgL that induces bulb to produce by it in the mode kept flat
-1+ TDZ 0.3mgL
-1+ IAA 0.5mgL
-1+ Ad 18mgL
-1in cultivated, condition of culture is: it is to cultivate 7 days under the condition of culture of 6 ℃ and unglazed photograph that culture materials is placed on;
S3: it is 20 ℃ that the culture materials after the S2 step process is placed on to cultivation temperature, and illumination every day 6h cultivates under the condition of culture that intensity of illumination is 400lx 20～40 days.
Embodiment 8: a kind of method that rapid induction Bulbus Fritillariae Cirrhosae bulb produces, and it comprises the following steps:
S1: choose the diameter of inducing generation and train bulb in the group of 2cm, access MS+KT 2mgL
-1+ NAA0.5 mgL
-1in medium, cultivate, condition of culture is: cultivation temperature is 16 ℃ and unglazed according to cultivating 35 days under condition, obtains the Bulbus Fritillariae Cirrhosae carnification spire produced by group training bulb;
S2: the carnification spire that to choose the spire upper end be white or yellow-white, from leaf sheath base cuts, access the medium MS+6-BA 2mgL that induces bulb to produce by it in the mode kept flat
-1+ TDZ 0.3mgL
-1+ IAA 0.5mgL
-1+ Ad 20mgL
-1in cultivated, condition of culture is: it is to cultivate 8 days under the condition of culture of 6 ℃ and unglazed photograph that culture materials is placed on;
S3: it is 25 ℃ that the culture materials after the S2 step process is placed on to cultivation temperature, and illumination every day 7h cultivates 40 days under the condition of culture that intensity of illumination is 500lx.
Embodiment 9: a kind of method that rapid induction Bulbus Fritillariae Cirrhosae bulb produces, and it comprises the following steps:
S1: choose the diameter of inducing generation and train bulb in the group of 0.5～2cm, access MS+KT 1～3mgL
-1+ NAA 0.1～1 mgL
-1in medium, cultivate, condition of culture is: cultivation temperature is 15 ℃ and unglazed according to cultivating 35 days under condition, obtains the Bulbus Fritillariae Cirrhosae carnification spire produced by group training bulb;
S2: the carnification spire that to choose the spire upper end be white or yellow-white, from leaf sheath base cuts, access the medium MS+6-BA 1～3mgL that induces bulb to produce by it in the mode kept flat
-1+ TDZ 0.1～0.5mgL
-1+ IAA 0.1～1mgL
-1+ Ad 10～25mgL
-1in cultivated, condition of culture is: it is to cultivate 7 days under the condition of culture of 6 ℃ and unglazed photograph that culture materials is placed on;
S3: it is 20 ℃ that the culture materials after the S2 step process is placed on to cultivation temperature, and illumination every day 6h cultivates under the condition of culture that intensity of illumination is 400lx 20～40 days.
Further illustrate effect of the present invention below by experiment:
Experiment one: the impact that the inventive method occurs bulb
S1 step: choose the diameter of inducing generation and train bulb in the group of 1.2 cm, access MS+KT 2mgL
-1+ NAA 0.5mgL
-1in medium, in cultivation temperature, be 15 ℃ and unglazed according to after cultivating 35 days under condition, obtain the Bulbus Fritillariae Cirrhosae carnification spire produced by group training bulb.The S2 step: choosing the spire upper end is white carnification spire, and it,, from leaf sheath base cuts, is accessed to the medium MS+6-BA 2mgL that induces bulb to produce in the mode kept flat
-1+ TDZ 0.3mgL
-1+ IAA 0.5mgL
-1+ Ad 18mgL
-1in cultivated, condition of culture is: it is to cultivate 7 days under the condition of culture of 6 ℃ and unglazed photograph that culture materials is placed on.The S3 step: it is 22 ℃ that the culture materials after the S2 step process is placed on to cultivation temperature, and illumination every day 6h cultivates 30 days under the condition of culture that intensity of illumination is 400lx.The generation bulb of Bulbus Fritillariae Cirrhosae carnification spire 100%, the bulb number that on average each spire explant produces is 15～30.
Experiment two: will induce the medium that group training bulb sprouts fast to change MS+6-BA 0.5mgL in experiment one S2 step
-1+ Cefotaxime Sodium 100mgL
-1in medium, other step is with experiment one; After 45 days cultivate, the sprout rate of group training bulb is 58.62%.
Experiment three: in experiment, will induce in one S2 step condition of culture that group training bulb sprouts fast to change into after cultivation temperature is to cultivate 45 days under 28 ℃, illumination every day 24 hours, the intensity of illumination condition of culture that is 2500lx, the sprout rate of group training bulb is 89.18%.
Experiment four: the medium in experiment one S3 step, inducing embryoid body produced changes MS+ Cefotaxime Sodium 500mgL into
-1, other step is with experiment one; After 50 days cultivate, the inductivity of its embryoid is 58.81%, and the embryoid shape produced mostly is elongated taper shape.
Experiment five: will organize the training bulb medium that sprouts fast in one S2 step in experiment and change the MS0 minimal medium into, and not add any phytohormone and antibiotic, other step is with testing one; After 45 days cultivate, the sprout rate of group training bulb is only 13.17%.
Experiment six: will test in a S2 step, after the condition of culture that group training bulb is sprouted fast changes into and cultivate 45 days under cultivation temperature is 15 ℃, the condition of unglazed photograph, other step is with testing one; The sprout rate of group training bulb is 11.85%.
Experiment seven: in experiment one S3 step, take not cut the yellow green blade part on spire top, other step is with experiment one, and after 50 days cultivate, the inductivity of its embryoid is 38.71%.
Experiment eight: in experiment one S3 step, the white leaf sheath of spire base portion is directly inserted in medium just to connect mode (with the morphology lower end of leaf sheath explant), other step is with experiment one, and after 50 days cultivate, the inductivity of its embryoid is 28.56%.
Experiment nine: in experiment one S3 step, the white leaf sheath of spire base portion is directly inserted in medium in reversal connection mode (with the morphology upper end of leaf sheath explant), other step is with real experiment one, and after 50 days cultivate, the inductivity of its embryoid is 39.16%.
Show by experiment, the inventive method has realized Direct Regeneration Bulbus Fritillariae Cirrhosae bulb from Bulbus Fritillariae Cirrhosae meat spire, for the rapid batch of Bulbus Fritillariae Cirrhosae medicinal part produces, provides a new way.The method can significantly improve the sprout rate of bulb, obviously shortens crop cycle, and then improves the output of Bulbus Fritillariae Cirrhosae.
Claims (7)
1. the method that a rapid induction Bulbus Fritillariae Cirrhosae bulb produces, it is characterized in that: it comprises the following steps:
S1: choose the diameter of inducing generation and train bulb in the group of 0.5～2cm, access MS+KT 1～3mgL
-1+ NAA 0.1～1 mgL
-1in medium, cultivate, condition of culture is: cultivation temperature is 12～18 ℃ cultivates 30～40 days according under condition with unglazed, obtains the Bulbus Fritillariae Cirrhosae carnification spire produced by group training bulb;
S2: the carnification spire that to choose the spire upper end be white or yellow-white, from leaf sheath base cuts, access the medium MS+6-BA 1～3mgL that induces bulb to produce by it in the mode kept flat
-1+ TDZ 0.1～0.5mgL
-1+ IAA 0.1～1mgL
-1+ Ad 10～25mgL
-1in cultivated, condition of culture is: it is to cultivate 5～10 days under the condition of culture of 4～8 ℃ and unglazed photograph that culture materials is placed on;
S3: it is 15～28 ℃ that the culture materials after the S2 step process is placed on to cultivation temperature, and illumination every day 4～8h cultivates under the condition of culture that intensity of illumination is 200～600lx 20～40 days.
2. the method that a kind of rapid induction Bulbus Fritillariae Cirrhosae bulb according to claim 1 produces is characterized in that: the group training bulb diameter described in the S1 step is 1.2cm, access MS+KT 2mgL
-1+ NAA 0.5mgL
-1medium in.
3. the method that a kind of rapid induction Bulbus Fritillariae Cirrhosae bulb according to claim 1 produces, it is characterized in that: the cultivation temperature described in the S1 step is 15 ℃.
4. the method that a kind of rapid induction Bulbus Fritillariae Cirrhosae bulb according to claim 1 produces is characterized in that: the condition of culture described in the S1 step is unglazed according to cultivating 35 days under condition.
5. the method that a kind of rapid induction Bulbus Fritillariae Cirrhosae bulb according to claim 1 produces is characterized in that: the medium of inducing bulb to produce described in the S2 step is MS+6-BA 2mgL
-1+ TDZ 0.3mgL
-1+ IAA 0.5mgL
-1+ Ad 18mgL
-1.
6. the method that a kind of rapid induction Bulbus Fritillariae Cirrhosae bulb according to claim 1 produces, it is characterized in that: the cultivation temperature described in the S2 step is: 6 ℃, and cultivate 7 days under the condition of culture of unglazed photograph.
7. the method that a kind of rapid induction Bulbus Fritillariae Cirrhosae bulb according to claim 1 produces, it is characterized in that: the cultivation temperature described in the S3 step is: 20 ℃, every day, light application time was 6h, under the condition of culture that intensity of illumination is 400lx, cultivated 30 days.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310437399.5A CN103493733B (en) | 2013-09-24 | 2013-09-24 | Method for quickly inducing generation of fritillaria cirrhosa bulb |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310437399.5A CN103493733B (en) | 2013-09-24 | 2013-09-24 | Method for quickly inducing generation of fritillaria cirrhosa bulb |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103493733A true CN103493733A (en) | 2014-01-08 |
| CN103493733B CN103493733B (en) | 2015-02-25 |
Family
ID=49859079
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310437399.5A Active CN103493733B (en) | 2013-09-24 | 2013-09-24 | Method for quickly inducing generation of fritillaria cirrhosa bulb |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103493733B (en) |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104957037A (en) * | 2015-07-07 | 2015-10-07 | 甘肃省科学院生物研究所 | Method for cultivating fritillaria przewalskii test-tube bulblet |
| CN105519435A (en) * | 2016-01-06 | 2016-04-27 | 南京海源中药饮片有限公司 | Thunberg fritillary bulb bulblet inducement tissue culture method |
| CN111296211A (en) * | 2020-03-12 | 2020-06-19 | 重庆市药物种植研究所 | Rapid propagation method of fritillaria taipaiensis bulbs |
| CN111492976A (en) * | 2020-05-18 | 2020-08-07 | 成都大学 | Method for culturing fleshy straight roots of bulbus fritillariae cirrhosae |
| CN111492977A (en) * | 2020-05-18 | 2020-08-07 | 成都大学 | Induced culture method of bulbus fritillariae cirrhosae round bulbs |
| CN112243631A (en) * | 2020-09-14 | 2021-01-22 | 云南省农业科学院花卉研究所 | Method for rapidly breaking dormancy of green flower lily seed bulbs |
| CN112931210A (en) * | 2021-03-18 | 2021-06-11 | 四川迪菲特药业有限公司 | Bulb proliferation culture method taking fritillaria paniculata bulb discs as explants |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114847159B (en) * | 2022-04-28 | 2023-04-07 | 云南龙藏生物科技有限公司 | Tissue culture regeneration method for bulbus fritillariae cirrhosae |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR100586495B1 (en) * | 2004-11-30 | 2006-06-07 | 삼성에버랜드 주식회사 | A method of embryogenic callus formation with a high frequency from bulb scale of tulip and shoot regeneration from the embryogenic callus |
| CN102002474A (en) * | 2009-09-03 | 2011-04-06 | 薛永新 | Rapid bulb multiplication method by using fritillaria cirrhosa leaves as explant |
| CN103238456A (en) * | 2013-05-31 | 2013-08-14 | 常熟市佳盛农业科技发展有限公司 | Method for utilizing fritillaria cirrhosa flakes to quickly breed fritillaria cirrhosa |
-
2013
- 2013-09-24 CN CN201310437399.5A patent/CN103493733B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR100586495B1 (en) * | 2004-11-30 | 2006-06-07 | 삼성에버랜드 주식회사 | A method of embryogenic callus formation with a high frequency from bulb scale of tulip and shoot regeneration from the embryogenic callus |
| CN102002474A (en) * | 2009-09-03 | 2011-04-06 | 薛永新 | Rapid bulb multiplication method by using fritillaria cirrhosa leaves as explant |
| CN103238456A (en) * | 2013-05-31 | 2013-08-14 | 常熟市佳盛农业科技发展有限公司 | Method for utilizing fritillaria cirrhosa flakes to quickly breed fritillaria cirrhosa |
Non-Patent Citations (2)
| Title |
|---|
| 王跃华等: "离体培养条件对卷叶贝母鳞茎再生的影响", 《中药材》, vol. 33, no. 6, 30 June 2010 (2010-06-30), pages 854 - 856 * |
| 陈敏等: "川贝母组织培养的研究", 《中国中药杂志》, vol. 20, no. 8, 31 December 1995 (1995-12-31), pages 461 - 463 * |
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104957037A (en) * | 2015-07-07 | 2015-10-07 | 甘肃省科学院生物研究所 | Method for cultivating fritillaria przewalskii test-tube bulblet |
| CN105519435A (en) * | 2016-01-06 | 2016-04-27 | 南京海源中药饮片有限公司 | Thunberg fritillary bulb bulblet inducement tissue culture method |
| CN111296211A (en) * | 2020-03-12 | 2020-06-19 | 重庆市药物种植研究所 | Rapid propagation method of fritillaria taipaiensis bulbs |
| CN111492976A (en) * | 2020-05-18 | 2020-08-07 | 成都大学 | Method for culturing fleshy straight roots of bulbus fritillariae cirrhosae |
| CN111492977A (en) * | 2020-05-18 | 2020-08-07 | 成都大学 | Induced culture method of bulbus fritillariae cirrhosae round bulbs |
| CN111492977B (en) * | 2020-05-18 | 2022-09-13 | 成都大学 | Induced culture method of bulbus fritillariae cirrhosae round bulbs |
| CN111492976B (en) * | 2020-05-18 | 2022-09-13 | 成都大学 | Method for culturing fleshy straight roots of bulbus fritillariae cirrhosae |
| CN112243631A (en) * | 2020-09-14 | 2021-01-22 | 云南省农业科学院花卉研究所 | Method for rapidly breaking dormancy of green flower lily seed bulbs |
| CN112931210A (en) * | 2021-03-18 | 2021-06-11 | 四川迪菲特药业有限公司 | Bulb proliferation culture method taking fritillaria paniculata bulb discs as explants |
| CN112931210B (en) * | 2021-03-18 | 2022-06-21 | 四川迪菲特药业有限公司 | Bulb proliferation culture method taking fritillaria paniculata bulb discs as explants |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103493733B (en) | 2015-02-25 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103493733B (en) | Method for quickly inducing generation of fritillaria cirrhosa bulb | |
| CN102002474B (en) | Rapid bulb multiplication method by using fritillaria cirrhosa leaves as explant | |
| CN102217540B (en) | Quick propagation method for lycoris chinensis | |
| CN102265787B (en) | Tissue culture method of president clematis | |
| CN102119655A (en) | Natural light rapid breeding method for dendrobium officinale | |
| CN104206270A (en) | Tissue culture and rhizome expansion method of Curcuma hybrida 'Red Torch' | |
| CN103518625B (en) | The tissue culture medium (TCM) of Root of Fiddleleaf Fig blade and in-vitro regeneration method | |
| CN102919122B (en) | High-efficiency method for inducing pinellia in vitro bulb | |
| CN103314979A (en) | Composition and method for raising chrysanthemum cutting survival rate | |
| CN101491216A (en) | Evodia fruit tissue-culture quick propagation method | |
| CN103609453A (en) | Construction method of in vitro regeneration system of tea tree | |
| CN102217538B (en) | Method for treating walnut tissue culture stem segments inside test tubes and rooting outside test tubes | |
| CN103609444B (en) | Tissue culture method for hemerocallis sempervirens araki | |
| CN103416302B (en) | Method for culturing regeneration plant of somatic embryo of osmanthus fragrans Lour | |
| CN103468633B (en) | Inducing method for improving cell synchronization of tendril-leaved fritillary bulbs | |
| CN103461137B (en) | Method for inducing generation of fritillaria cirrhosa embryoids | |
| CN102754599B (en) | Method for quickly breeding cymbidium hybridium by use of root inducing protocorm | |
| CN102511393A (en) | Method for establishing racomitrium japonicum gametophyte regeneration system | |
| CN102067818A (en) | Inducing technology of test tube lotus root | |
| CN102630566B (en) | Method for cultivation and production of paclitaxel from induction of taxus chinensis var. mairei axillary bud in vitro | |
| CN103858760A (en) | Manufacturing method of test-tube type anoectohilus formosanus | |
| CN103250639A (en) | Dendrobium officinale seed cultivation method | |
| CN103444547A (en) | Culture method of aralis suspension cells | |
| CN103004404A (en) | Anthurium plantlet culturing method | |
| CN106718940A (en) | A kind of tissue culture and rapid propagation method suitable for tung oil tree |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |