CN103483303A - Proanthocyanidin extraction method - Google Patents
Proanthocyanidin extraction method Download PDFInfo
- Publication number
- CN103483303A CN103483303A CN201210202895.8A CN201210202895A CN103483303A CN 103483303 A CN103483303 A CN 103483303A CN 201210202895 A CN201210202895 A CN 201210202895A CN 103483303 A CN103483303 A CN 103483303A
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- CN
- China
- Prior art keywords
- pycnogenols
- extracting method
- starting material
- ethanolic solution
- aqueous ethanolic
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D311/00—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only hetero atom, condensed with other rings
- C07D311/02—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only hetero atom, condensed with other rings ortho- or peri-condensed with carbocyclic rings or ring systems
- C07D311/04—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring
- C07D311/58—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring other than with oxygen or sulphur atoms in position 2 or 4
- C07D311/60—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring other than with oxygen or sulphur atoms in position 2 or 4 with aryl radicals attached in position 2
- C07D311/62—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring other than with oxygen or sulphur atoms in position 2 or 4 with aryl radicals attached in position 2 with oxygen atoms directly attached in position 3, e.g. anthocyanidins
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- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Extraction Or Liquid Replacement (AREA)
Abstract
The invention discloses a proanthocyanidin extraction method. The method is characterized in that proanthocyanidin is extracted through a diafiltration process under the ultrasonic effect in a container provided with an ultrasonic generating device, is further purified, and is dried to obtain a powdery product.
Description
Technical field
The present invention relates to a kind of extracting method of pycnogenols.
Background technology
The pycnogenols polyphenolic substance general name that to be a class formed by catechin or the l-Epicatechol condensation of different quantities, nonpoisonous and tasteless, there is powerful anti-oxidant activity and radical scavenging activity, there is hypotensive, reducing blood-fat, prevent arteriosclerosis, the effect such as anticancer, anti-ageing and beauty treatment, be used in disease treatment, healthcare products and cosmetic field, become the natural plant extracts that liked by the various countries people.Therefore people more and more pay attention to the research of pycnogenols, and finding the new starting material that are rich in pycnogenols is two study hotspots with the method that is suitable for industrialization extraction, detection pycnogenols.
At present, the extraction process about pycnogenols mainly contains soxhlet extraction, heat reflow method, ultrasonic extraction and supercritical extraction.Although soxhlet extraction has high extraction yield, is not suitable for suitability for industrialized production; The heat reflow method industrialized producing technology is simple, but the destruction that heating easily causes effective constituent, yield is also not as good as soxhlet extraction; Ultrasonic extraction has short advantage of time, but yield is also not as good as soxhlet extraction; Although supercritical extraction has the advantages such as environmental friendliness, product be easily separated, energy consumption is higher.
Summary of the invention
The object of the present invention is to provide a kind of extracting method of pycnogenols, in order to realize this purpose, the present invention has adopted following concrete technological step:
(1) will join containing the starting material of pycnogenols in the container that ultrasonic generator (0-400W) is housed of certain capacity;
(2) 10-20min under ul-trasonic irradiation, adopt the diafiltration method that the aqueous ethanolic solution of 20%-80% ratio is passed through to starting material by certain flow rate;
(3) reaction gained mixture is through concentrating, be drying to obtain target product.
As a kind of improvement, described container is equipped with ultrasonic generator.
As a kind of improvement, described diafiltration method is to carry out under ultrasonication.
As a kind of improvement, after purifying, also comprise that freeze-drying makes the step of powdered product.
The beneficial effect that the present invention has is:
(1) diafiltration method is extracted pycnogenols, and easy and simple to handle saving time, reduced cost;
(2) use ultrasonic wave auxiliary, greatly accelerated extraction rate, efficiency of pcr product also is improved;
(3) use aqueous ethanolic solution safe, nontoxic, that be easy to get to make solvent, convenient operation and processing, ethanol easily reclaims use.
Embodiment
Below by specific embodiment, the invention will be further described:
Under embodiment 1 ultrasonic wave is auxiliary, extract pycnogenols in Cortex Pini in ethanol current hydrodynamic
In the 50mL container, add a certain amount of Cortex Pini, the pillar 20min that flows through under the 300W ul-trasonic irradiation of the aqueous ethanolic solution with 80%, collect extracting solution, and concentrated, freeze-drying obtains powder, and the pycnogenols extraction yield is 97%.
Under embodiment 2 ultrasonic wave are auxiliary, in ethanol current hydrodynamic, extract Proanthocyanidins from Grape Seeds
In the 50mL container, add a certain amount of Semen Vitis viniferae, the pillar 15min that flows through under the 250W ul-trasonic irradiation of the aqueous ethanolic solution with 65%, collect extracting solution, and concentrated, freeze-drying obtains powder, and the pycnogenols extraction yield is 89%.
Under embodiment 3 ultrasonic wave are auxiliary, extract pycnogenols in purple sweet potato in ethanol current hydrodynamic
In the 50mL container, add a certain amount of Semen Vitis viniferae, the pillar 10min that flows through under the 200W ul-trasonic irradiation of the aqueous ethanolic solution with 35%, collect extracting solution, and concentrated, freeze-drying obtains powder, and the pycnogenols extraction yield is 0.9%.
Under embodiment 4 ultrasonic wave are auxiliary, extract pycnogenols in Testa arachidis hypogaeae in ethanol current hydrodynamic
In the 50mL container, add a certain amount of Semen Vitis viniferae, the pillar 10min that flows through under the 200W ul-trasonic irradiation of the aqueous ethanolic solution with 50%, collect extracting solution, and concentrated, freeze-drying obtains powder, and the pycnogenols extraction yield is 12%
Under embodiment 5 ultrasonic wave are auxiliary, extract pycnogenols in rapeseed hull in ethanol current hydrodynamic
In the 50mL container, add a certain amount of Semen Vitis viniferae, the pillar 20min that flows through under the 300W ul-trasonic irradiation of the aqueous ethanolic solution with 75%, collect extracting solution, and concentrated, freeze-drying obtains powder, and the pycnogenols extraction yield is 2.6%
Finally, it is also to be noted that, what more than enumerate is only specific embodiments of the invention.Obviously, the invention is not restricted to above examples of implementation, many distortion can also be arranged.All distortion that those of ordinary skill in the art can directly derive or associate from content disclosed by the invention, all should think protection scope of the present invention.
Claims (5)
1. the extracting method of a pycnogenols, is characterized in that, comprises the following steps:
(1) will join containing the starting material of pycnogenols in the container that ultrasonic generator (0-400W) is housed of certain capacity;
(2) 10-20min under ul-trasonic irradiation, adopt the diafiltration method that the aqueous ethanolic solution of 20%-80% ratio is passed through to starting material by certain flow rate;
(3) reaction gained mixture is through concentrating, be drying to obtain target product.
2. the extracting method of pycnogenols according to claim 1, is characterized in that, starting material can be all solids shape materials containing pycnogenols.
3. the extracting method of pycnogenols according to claim 1, is characterized in that, described percolating device has ultrasonic generating function.
4. the extracting method of pycnogenols according to claim 1, is characterized in that, adopts the diafiltration method that a certain proportion of aqueous ethanolic solution is extracted by starting material by certain flow rate.
5. the extracting method of pycnogenols according to claim 1, is characterized in that, after purifying, also comprises that freeze-drying makes the step of powdered product.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210202895.8A CN103483303A (en) | 2012-06-13 | 2012-06-13 | Proanthocyanidin extraction method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210202895.8A CN103483303A (en) | 2012-06-13 | 2012-06-13 | Proanthocyanidin extraction method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103483303A true CN103483303A (en) | 2014-01-01 |
Family
ID=49823952
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201210202895.8A Pending CN103483303A (en) | 2012-06-13 | 2012-06-13 | Proanthocyanidin extraction method |
Country Status (1)
| Country | Link |
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| CN (1) | CN103483303A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111518860A (en) * | 2020-05-09 | 2020-08-11 | 浙江天草生物科技股份有限公司 | Preparation method of cowberry fruit extract |
-
2012
- 2012-06-13 CN CN201210202895.8A patent/CN103483303A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111518860A (en) * | 2020-05-09 | 2020-08-11 | 浙江天草生物科技股份有限公司 | Preparation method of cowberry fruit extract |
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| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20140101 |