CN103479538A

CN103479538A - External preparation composition for skin comprising ginseng flower or ginseng seed extracts

Info

Publication number: CN103479538A
Application number: CN201310314216.0A
Authority: CN
Inventors: 廉明勳; 李真锳; 黄贞善; 朴录贤; 朴葰星; 金德姬; 金汉坤
Original assignee: Amorepacific Corp
Current assignee: Amorepacific Corp
Priority date: 2008-06-13
Filing date: 2009-04-28
Publication date: 2014-01-01
Anticipated expiration: 2029-04-28
Also published as: CN103479694B; US8709511B2; JP5628797B2; JP2011522881A; CN103479538B; CN103479694A; CN102065836A; US20110085996A1

Abstract

Provided are a use of a ginseng flower extract and a use of a ginseng seed extract. The ginseng seed extract includes ginseng seed oil or a ginseng seed residue extract. The uses include skin protection, anti-aging, anti-wrinkle, improvement of skin elasticity, skin whitening, skin moisturizing, prevention and improvement of skin dryness diseases such as atopy, removal of the horny layer, sebum control, anti-inflammation, prevention or improvement of skin troubles such as acne, improvement of skin complexion, prevention of hair loss, anti-dandruff, improvement of gloss and softness of hair, improvement of volume and elasticity of face or body, and improvement of facial or body line.

Description

The Dermatologic preparation composition of the flower that contains Radix Ginseng or the seed extract of Radix Ginseng

The application is to be dividing an application of April 28, application number in 2009 are 200980122190.1, denomination of invention is " Dermatologic preparation composition of the flower that contains Radix Ginseng or the seed extract of Radix Ginseng " application for a patent for invention the applying date.

Technical field

The present invention relates to the purposes of the seed extract of the purposes of flower extract of Radix Ginseng and Radix Ginseng.The seed extract of Radix Ginseng comprises the seed oil of Radix Ginseng or the seed slag extract of Radix Ginseng.In more detail, the present invention relates to the skin care purposes of the seed extract of the flower extract of Radix Ginseng or Radix Ginseng, the aging resistance purposes, the anti-wrinkle purposes, skin elasticity is improved purposes, the whitening purposes, the skin moisture-keeping purposes, the prevention of the xerosis cutis diseases such as atopy and improve purposes, sebum is regulated purposes, the anti-inflammatory purposes, the prevention of the skin problems such as acne or improve purposes, except exfoliation, improve the skin color, pre-Anti-hair loss, anti-dandruff, the gloss of hair and the improvement of flexibility, the volume of face or health or elastic enhancement, the improvement of the lines of face or health.

Background technology

Radix Ginseng (Panax ginseng C.A.Meyer) is the plant that belongs to the Araliaceae Panax, in areas such as Korea S, China, Japan, is the crude drug just be used before more than 2000 years, rule of thumb, take prevent disease and life-saving to be used as purpose.Radix Ginseng known function and effect up to now has: for central nervous system's effect, antitumaous effect and active anticancer, Immunologic Functions, anti-glycosuria effect, strengthen the liver function function, cardiovascular disorder improves and arteriosclerosis effect, Blood pressure regulation, relate to the climacteric obstacle and improve and osteoporotic effect, compressive resistance and antifatigue effect, antioxidant activity and aging inhibit feature etc.

In the aerial parts of Radix Ginseng and under ground portion, distribution is all arranged as the ginsenoside (Ginsenoside) of the representational physiologically active ingredient of Radix Ginseng.But the fruit of the root of Radix Ginseng, the leaf of Radix Ginseng, Radix Ginseng etc. are according to the difference at its position, ginsenoside's content and composition have larger difference.It is reported, especially the flower of Radix Ginseng be take the ginsenoside component content different from the root of Radix Ginseng and composition as basis, has the good liver-protecting function (J.Nat.Prod, 66 different from other positions; 922-927,2003.).On the other hand, in the situation that the seed of Radix Ginseng, owing to only being used as the means for cultivated ginseng, therefore the research of contained seed oil in the seed about the seed of Radix Ginseng, particularly Radix Ginseng, the fatty acid that only limits to the seed oil of Radix Ginseng forms and the content analysis (J.Agric.Food Chem.50,744-750,2002) of phytosterol.

Recently, consumer constantly increases for the care degree of natural cosmetics, and utilizes a lot of cosmetics of Chinese crude drug to be sold, and wherein, Radix Ginseng is the plant material of important skin function and studied as having of cosmetics also.But wherein major part has been utilized the root of Radix Ginseng.Particularly, practical situation is: be only to have utilized ginsenoside's composition of root of the extract of root of Radix Ginseng or Radix Ginseng and the polysaccharide of Radix Ginseng, and the research of the skin function do not brought about the composition of the seed of the flower by Radix Ginseng or Radix Ginseng fully.

Summary of the invention

The object of the present invention is to provide a kind of skin preparations for extenal use useful to skin.

The purpose of an embodiment of the invention is to provide a kind of compositions of protecting skin.

The purpose of another embodiment of the invention is to provide a kind of compositions of protecting hair.

The purpose of another embodiment of the invention is to provide a kind of aging compositions of preventing or improve.

The purpose of another embodiment of the invention is to provide a kind of compositions of preventing or improving wrinkle.

The purpose of another embodiment of the invention is to provide a kind of compositions of improving skin elasticity.

The purpose of another embodiment of the invention is to provide a kind of skin-whitening compositions.

The purpose of another embodiment of the invention is to provide a kind of skin moisture-keeping compositions.

The purpose of another embodiment of the invention is to provide a kind of compositions of preventing or improving the xerosis cutis diseases such as atopy.

The purpose of another embodiment of the invention is to provide a kind of compositions of removing skin keratin.

The purpose of another embodiment of the invention is to provide a kind of compositions of sebum of regulation of skin.

The purpose of another embodiment of the invention is to provide a kind of compositions of preventing or reducing inflammation.

The purpose of another embodiment of the invention is to provide a kind of inflammation or abirritant compositions of preventing or alleviating scalp.

The purpose of another embodiment of the invention is to provide a kind of compositions of preventing or improving the skin problems such as acne.

The compositions that the purpose of another embodiment of the invention is to provide a kind of prevention or improves the skin problems such as acne that occur on scalp.

The purpose of another embodiment of the invention is to provide a kind of compositions of improving the color of skin.

The purpose of another embodiment of the invention is to provide a kind of pre-Anti-hair loss compositions.

The purpose of another embodiment of the invention is to provide a kind of anti-dandruff compositions.

The purpose of another embodiment of the invention is to provide a kind of gloss of hair or compositions of flexibility of promoting.

The purpose of another embodiment of the invention is to provide a kind of volume and elastic compositions of promoting face or health.

The purpose of another embodiment of the invention is to provide a kind of compositions of improving the lines of face or health.

To achieve these goals, the flower extract that Dermatologic preparation composition involved in the present invention contains Radix Ginseng or the seed extract of Radix Ginseng are as effective ingredient.

In an embodiment of the invention, in related Dermatologic preparation composition, the seed oil that the seed extract of above-mentioned Radix Ginseng is Radix Ginseng gets final product.Under the seed oil condition that is Radix Ginseng at the seed extract of above-mentioned Radix Ginseng, preferably its content is 0.001～100 % by weight with respect to the gross weight of Dermatologic preparation composition.

At another embodiment of the invention, in related Dermatologic preparation composition, the seed slag extract that the seed extract of above-mentioned Radix Ginseng is Radix Ginseng gets final product.The content of the seed slag extract of preferred above-mentioned Radix Ginseng is 0.001～10 % by weight with respect to the gross weight of Dermatologic preparation composition.

In an embodiment of the invention, Dermatologic preparation composition is characterised in that to have the purposes that suppresses skin injury and protect skin.At this, skin comprises scalp or hair.That is, the damage that suppresses scalp or hair the Dermatologic preparation composition of protecting hair are disclosed in this manual.

In yet another embodiment of the present invention, Dermatologic preparation composition is characterised in that, by suppressing to be used for protecting skin by oxidation or UV-induced skin injury.At this, skin comprises scalp or hair.That is, disclose by suppressing to be protected by the damage of oxidation or UV-induced scalp or hair the Dermatologic preparation composition of scalp or hair.

In yet another embodiment of the present invention, Dermatologic preparation composition is characterised in that to have the purposes of preventing or improving skin aging.

In yet another embodiment of the present invention, Dermatologic preparation composition is characterised in that, by suppressing by oxidation or UV-induced skin injury, promoting collagenation or hinder matrix metalloproteinase I(MMP-1) biosynthesis suppress the aging of skin.

The related Dermatologic preparation composition of another embodiment of the invention is characterised in that to have the purposes of preventing or improving wrinkle of skin.

The related Dermatologic preparation composition of another embodiment of the invention is characterised in that, by promoting collagenation or hindering matrix metalloproteinase I(MMP-1) biosynthesis prevent or improve wrinkle of skin.

The related Dermatologic preparation composition of another embodiment of the invention is characterised in that to have the purposes of improving skin elasticity.

The related Dermatologic preparation composition of another embodiment of the invention is characterised in that to have the purposes of skin-whitening.

The related Dermatologic preparation composition of another embodiment of the invention is characterised in that, generates the pigment whitening that maybe will be deposited on skin by check melanin and carries out skin-whitening.

The related Dermatologic preparation composition of another embodiment of the invention is characterised in that to have the purposes of skin moisture-keeping.

The related Dermatologic preparation composition of another embodiment of the invention is characterised in that, by the differentiation of promotion keratinocyte or the expression of promotion transglutaminase, keeps moisture of skin.

The related Dermatologic preparation composition of another embodiment of the invention is characterised in that to have prevention or improve the purposes that comprises atopy or psoriasic xerosis cutis disease.

In yet another embodiment of the present invention, Dermatologic preparation composition is characterised in that to have the purposes except exfoliation.

The related Dermatologic preparation composition of another embodiment of the invention is characterised in that to have the purposes of the sebum of regulation of skin.

The related Dermatologic preparation composition of another embodiment of the invention is characterised in that, carrys out the sebum of regulation of skin by the secretion that suppresses sebum.

The related Dermatologic preparation composition of another embodiment of the invention is characterised in that have prevention or improve inflammation or abirritant purposes.At this, skin comprises scalp.That is, disclose in this manual the inflammation that has the prevention or improve scalp, alleviated the Dermatologic preparation composition to the purposes of the stimulation of scalp.

The related Dermatologic preparation composition of another embodiment of the invention is characterised in that to have the purposes that prevention or improvement comprise the skin problem of acne.At this, skin comprises scalp.That is, provide in this manual and there is the skin problem that prevents to comprise acne on scalp, the Dermatologic preparation composition that improves the purposes of the skin problem occurred.

In yet another embodiment of the present invention, Dermatologic preparation composition is characterised in that to have the purposes of improving the skin color.

In yet another embodiment of the present invention, Dermatologic preparation composition is characterised in that to have the purposes of pre-Anti-hair loss.

In yet another embodiment of the present invention, Dermatologic preparation composition is characterised in that to have the purposes of anti-dandruff.

In yet another embodiment of the present invention, Dermatologic preparation composition is characterised in that to have the gloss of enhancement hair or the purposes of flexibility.

In yet another embodiment of the present invention, Dermatologic preparation composition is characterised in that to have volume and the elastic purposes of promoting face or health.

In yet another embodiment of the present invention, Dermatologic preparation composition is characterised in that to have the purposes of the lines that improve face or health.

In the present invention, owing to containing the seed extract that has with the flower extract of the Radix Ginseng of the differentiated composition of the root of Radix Ginseng and composition or Radix Ginseng as effective ingredient in the aerial parts of Radix Ginseng, thereby there is the effect that following compositions can be provided, , said composition can be carried out skin care, aging resistance, anti-wrinkle, skin elasticity is improved, whitening, skin moisture-keeping, prevention and the improvement of the xerosis cutis diseases such as atopy, cutin is removed, sebum is regulated, anti-inflammatory, the prevention of the skin problems such as acne or improvement, the skin color is improved, pre-Anti-hair loss, anti-dandruff, the gloss of hair and the improvement of flexibility, the volume of face or health and elastic enhancement, the improvement of the lines of face or health.

The accompanying drawing explanation

Fig. 1 means that the active oxygen of the flower extract of the Radix Ginseng that an embodiment of the invention are related generates the chart that suppresses experimental result.

Fig. 2 means that the DNA oxidative damage of the flower extract of the Radix Ginseng that an embodiment of the invention are related suppresses active chart.

Fig. 3 means that the flower extract of the Radix Ginseng that an embodiment of the invention are related relates to the chart of the effect of I type procollagen generation.

Fig. 4 means the chart of the flower extract of the Radix Ginseng that an embodiment of the invention are related to the impact effect of the expression of MMP-1.

Fig. 5 means the chart of the impact effect that the flower extract of the Radix Ginseng that an embodiment of the invention are related generates melanin.

Fig. 6 means the chart of the inhibition that the related seed extract by Radix Ginseng of an embodiment of the invention generates melanin.

The specific embodiment

In this manual, so-called " extract ", the material obtained so long as extract composition wherein from natural goods gets final product, irrelevant with the kind of extracting method and composition, comprises all above-mentioned substances.For example, the concept of its broad sense that is the material that comprises that material that material that the composition that utilizes water or organic solvent to extract to be dissolved in solvent obtains, the specific composition of specific composition such as wet goods that only extracts natural goods obtain etc. is all from natural goods.

In this manual, the meaning of so-called " seed oil of Radix Ginseng " is, the oil obtained from the seed of Radix Ginseng.For example, can be the extract that utilizes the organic solvents such as hexane, ether, dichloromethane to extract from the seed of Radix Ginseng.

In this manual, the meaning of so-called " the seed slag of Radix Ginseng " is to remove the rear residual residual fraction that deoils from the seed of Radix Ginseng.For example, can be to utilize the organic solvents such as hexane, ether, dichloromethane to extract the rear residual residual fraction of oil from the seed of Radix Ginseng.

In this manual, the meaning of so-called " skin " is, covers the tissue of the body surface of animal, and it is not only to comprise the tissue of body surfaces such as covering face or health and comprise scalp and the concept of the broad sense of hair.

In embodiments of the present invention, above-mentioned Radix Ginseng is Radix Ginseng (Panax ginseng C.A.Meyer).

In embodiments of the present invention, the flower extract of above-mentioned Radix Ginseng, according to the form of Dermatologic preparation composition, can be take and be mixed as the amount of 0.001～10 % by weight with respect to the gross weight of compositions, still need not be limited to this.

The flower extract of the Radix Ginseng that embodiments of the present invention are related can be by the organic solvent (ethanol, methanol, butanols, ether, ethyl acetate, chloroform etc.) or the organic solvent that add water, contain water with the amount of 1～10 times (wt/v%) of the flower of Radix Ginseng in the spending of the Radix Ginseng dry, and places at normal temperatures over 1 day and prepare and get final product.Preferably repeat 2 identical operations and obtain extracting solution.In addition, preferably can with vacuum decker, its filtrate be concentrated by remove chlorophyll from extracting solution after, obtain the dry thing of the flower extract of Radix Ginseng.

In yet another embodiment of the present invention, the seed oil that the seed extract of above-mentioned Radix Ginseng is Radix Ginseng, according to the form of Dermatologic preparation composition, this oil can be take and mixed as the amount of 0.001～100 % by weight with respect to the gross weight of compositions.During seed oil less than 0.001 % by weight of above-mentioned Radix Ginseng, it is very small that its effect becomes.

In yet another embodiment of the present invention, the seed oil of above-mentioned Radix Ginseng can be by after pulverizing the seed of dry Radix Ginseng, put into the organic solvent (hexane, ether, dichloromethane etc.) of 1～10 times (wt/v%) of this ground product, and place at normal temperatures over 1 day and extracted.For example, can obtain extracting solution by repeating 3 identical operations, after filtering extracting solution, with vacuum decker, its filtrate be concentrated, obtain the seed oil of Radix Ginseng.

In an embodiment of the invention in related Dermatologic preparation composition, according to the form of Dermatologic preparation composition, the seed slag extract of above-mentioned Radix Ginseng can be take and mixed as the amount of 0.001～10 % by weight with respect to the gross weight of compositions.During seed slag extract less than 0.001 % by weight of Radix Ginseng, it is very small that its effect becomes; While surpassing 10 % by weight, with the increase of content, compare, the increase of effect is little.

The seed slag extract of above-mentioned Radix Ginseng prepares and gets final product by following method.After the seed of dry Radix Ginseng is pulverized, put into the organic solvents such as hexane of 1～10 times (wt/v%) of this ground product, and place at normal temperatures over 1 day and extracted.Can be by repeating 3 identical operations, remove and deoil from the seed of Radix Ginseng, add the water of 1～10 times (wt/v%), the organic solvent that contains water (ethanol, methanol, butanols, ether, ethyl acetate, chloroform etc.) or organic solvent in the seed slag of the Radix Ginseng except residual after deoiling, and place at normal temperatures over 1 day and extracted.Can obtain extracting solution by repeating 3 identical operations, after filtering extracting solution, with vacuum decker, its filtrate be concentrated, obtain the dry thing of the seed slag extract of Radix Ginseng.

People's skin with advancing age, because various endogenous causes of ill, outer thereby experience change.That is, aspect endogenous cause of ill, owing to regulating metabolic various hormonal secretions, reduce, the function of immunocyte and the activity of cell descend, thereby the biosynthesis that the required immune protein of organism and organism form protein reduces; Aspect exopathogenic factor, owing to being accompanied by the sunray brought by the destruction of ozone layer, the ultraviolet content that arrives earth's surface increases and seriousization of environmental pollution, the increases such as free radical and active harmful oxygen, therefore, various variations have been caused, the not only thickness attenuation of skin, wrinkle increases, flexibility decrease, and color variation, skin becomes and seems to become dim, and skin problem is multiple, moth patch or freckle and senile plaque increase etc.

Be accompanied by aging carrying out, occurred as the content of collagen, elastin laminin, hyaluronic acid and the glycoprotein of the material that forms skin and the symptom that configuration changes or reduces, thereby faced the oxidative pressure brought by free radical and active harmful oxygen.In addition, be known that: due to aging carrying out and ultraviolet, in the most cell that forms skin, the enzyme that generates the proinflammatory cytokine (proinflammatory cytokine) of known initiation inflammation is COX-2 (Cox-2, cyclooxygenase) biosynthesis increases, the enzyme that decomposes skin histology by these inflammatory factors is matrix metalloproteinase (MMP, Matrix metalloproteinase) biosynthesis increases, by iNOS(inducible nitric oxide synthase, the inducible nitric oxide synthase) the NO(nitric oxide carried out, nitric oxide) generation increases.; minimizing and micro-inflammation due to the aging cytoactive caused of the endogenous by naturally carrying out; make the biosynthesis of stroma ground substance reduce; the exopathogenic factors such as increase due to the increase of the pressure brought by various hostile environments and the reactive oxygen species that brought by sunray; make and decompose and the degeneration accelerationization; dermal matrix is destroyed, attenuation, and show the various symptoms of skin aging.

In this description, disclosed Dermatologic preparation composition has the generation that suppresses active oxygen and suppresses seed extract by the flower extract of the Radix Ginseng of the function of the oxidative damage of Cells Induced by Reactive Oxygen Species or Radix Ginseng as effective ingredient by containing; can prevent the damage of the cell that caused by active oxygen or ultraviolet etc.; consequently, can protect skin.In addition, can suppress by active oxygen or UV-induced aging carrying out.And then the flower extract of Radix Ginseng and the seed extract of Radix Ginseng, by suppressing the biosynthesis of COX-2, can prevent or improve inflammation, consequently, can alleviate skin problem.At this, so-called skin comprises scalp.That is, in this description the seed extract of the flower extract of disclosed Radix Ginseng or Radix Ginseng can with damage, the prevention of the prevention cell caused by active oxygen or ultraviolet etc. that occur on scalp or hair or improve scalp inflammation, alleviate the scalp caused by hair-waving or hair dyeing etc. stimulation purposes and be used.In addition, in this description the seed extract of the flower extract of disclosed Radix Ginseng or Radix Ginseng can also with the prevention or improve skin wrinkle purposes and be used.

The colour of skin for the decider, various factors is all relevant, but, wherein, produce activeness, the blood vessel of the melanocyte (melanocyte) of melanin pigment distribution, skin thickness and to have or not the inside and outside factors such as pigment such as the human body that contains carotenoid, bilirubin be important.

Wherein, the factor of particular importance is to be called melanic black pigment, in the melanocyte of this melanin in human body, by plurality of enzymes effects such as tryrosinases, generates.About the formation of this melanin pigment, environment sexual factor such as the Physiological factors relevant to hereditary factor, hormone secretion, pressure etc. and ultraviolet irradiation etc. is all influential.

The melanin pigment generated in the melanocyte of application on human skin is that the phenol with complex form of the pigment of black and protein is polymer substance; play a part the useful of protection skin internal protein and gene; for example; the ultraviolet of blocking-up from shining upon; protect subepidermal skin organ, the free radical that simultaneously will produce in skin organism body etc. is caught etc.

Disappear in by skin keratin and be discharged to before outside the stable material that also can not disappear even the melanin that the stimulation by the pressure inside and outside such skin forms is pressure.But, while forming the melanin more than the as many as necessary amount, bring out the pigments precipitation diseases such as moth patch or freckle, speckle, cause the upper bad result of beauty treatment.

In this description, the seed extract of the flower extract of disclosed Radix Ginseng or Radix Ginseng can make skin-whitening by the generation of check melanin.Consequently, the flower extract that contains Radix Ginseng or the seed extract of Radix Ginseng as effective ingredient, disclosed Dermatologic preparation composition can be used as the purposes of skin-whitening in this description.

In skin; the most important function that is positioned at outermost epidermis is; for the defence of the various stimulations from outside (stimulating factor of the physics such as chemical substance, Air Pollutants, dry environment, ultraviolet, chemical) with prevent the excessive defencive function of dispersing from moisture in the body of skin, such defencive function can have been given play to when the horny layer consisted of keratinocyte normally starts to form.Even in epidermis, be present in outermost horny layer and formed by keratinocyte, the horn cell that has comprised differentiation and its lipid layer of covering.Horn cell is that the basal cell of breeding constantly at the epidermis orlop is periodically through the variation on morphology and function and rise to the distinctive cell of skin surface; After the time of defined, old horn cell is from skin exfoliation, and new horn cell is being born its function, and a series of change procedures of such repetition are called " differentiation of epidermis cell " or " keratinization (keratinization) ".In the keratinization process, the keratinocyte limit generates nature moisturizing factor (Natural Moisturizing Factor; NMF) and intercellular lipid (ceramide, cholesterol, fatty acid), limit forms horny layer, because this horny layer has robustness and flexibility, therefore plays the function as skin barrier (skin barrier).

But, such horny layer because excessive washing one's face, the factor of the factor of the living habit such as bathing or the environment such as dry atmosphere, polluter and the endogenous diseases such as atopy skin or geroderma etc., its function is easily lost, in fact, enter the modern times, due to the more factor further increased, recently, the people of complaint dry skin symptom and the various obstacles that bring thus is in continuous increase.Therefore, to supply with moisture in order to keep suitable moisture of skin from outside or constantly to increase from the research quantity of the moisture loss in body in order preventing, in fact, a lot of wetting agent with moisture-retaining capacity is developed, and mainly is used to cosmetic field.

But, in living environment, hazard factor for the people constantly increases, in addition, due to the surge that is accompanied by the aged, (turnover rate) is slack-off for cuticular generation-falling speed, the lipid synthesis ability of keratinocyte reduces, perhaps, the division of the normal cell in epidermis, maturation and differentiation can not successfully be carried out, therefore, have amount in cuticular Moisture factor and lipid reduce and can not keep normal cuticular function state, be that the people of the skin of the skin barrier function state of being disintegrated increases.

Because division, the differentiation of so abnormal epidermis cell causes skin to bring out the various dermatosis such as xeroderma, atopy, psoriasis, such various diseases is only by being used the common wetting agent that only has moisture-keeping functions, can alleviate a little its symptom, but truth is to be difficult to expect radical cure.

In this description, the seed extract of the flower extract of disclosed Radix Ginseng or Radix Ginseng is by promoting the differentiation of keratinocyte, contribute to coming off and " differentiation of epidermis cell " or " keratinization " process of the generative process of new horn cell as old horn cell, consequently, have and can effectively remove the function of exfoliation.In this description, disclosed Dermatologic preparation composition has seed extract that above-mentioned good cutin removes the flower extract of Radix Ginseng of function or Radix Ginseng as effective ingredient by containing, and can effectively as cutin, remove and use compositions.

In addition, in this description, the seed extract of the flower extract of disclosed Radix Ginseng or Radix Ginseng remains in normal range by the speed (turn over rate) by cuticular generation-come off, thereby the amount of cuticular Moisture factor and lipid normally can be kept.In addition, promote transglutaminase to express.Consequently, the moisturizing of skin becomes possibility.

In addition, in this description, the seed extract of the flower extract of disclosed Radix Ginseng or Radix Ginseng is by the differentiation and the maintenance moisture of skin that promote keratinocyte, skin barrier function can be strengthened, consequently, the xerosis cutis diseases such as atopy or psoriasis can be prevented or treat.

Usually, comprise that moisturizing that sebum in the skin of scalp and face plays a part skin keeps and prevent the intrusion of microorganism, still, during the sebum excessive secretion, promote alopecia, acne worsens, the expansion of the pore that promotion is caused by acne, bring out various diseases, such as seborrheic dermatitis etc. occurs.

The excessive secretion of such sebum is caused by a variety of causes, but, wherein the most important thing is, according to as in the activity of sebaceous gland to the amount of the dihydrotestosterone (dihydrotestosterone:DHT) that promotes one of hormone that sebum secretion is relevant, the sebaceous gland cell activation, the sebum excessive secretion.; in alopecia; in cell; testosterone (testosterone:T) converts the dihydrotestosterone as one of androgen to by 5-5 alpha-reductases 2 types (5-α-reductase type2); simultaneously; with at intracytoplasmic receptor protein, be combined, enter in core, bring out alopecia; but; in skin and sebaceous gland, testosterone converts dihydrotestosterone, sebaceous gland cell activation to by 5-5 alpha-reductases 1 type (5-α-reductase type1); differentiation is promoted; thus, the sebum (sebum) in the excessive secretion sebaceous gland, bring out acne.

Except the excessive secretion of simple sebum, as percutaneous Microinflammations such as the acne of the problem produced on skin, alopecias, further worsen.From the generation reason of acne, have and be accumulated in hair follicle the stage that the activity that causes acne bacteria activated and brought out inflammation because of superfluous sebum.

In this description, the seed extract of the flower extract of disclosed Radix Ginseng or Radix Ginseng is by suppressing sebum secretion, can prevent or improve acne that the excessive secretion by sebum causes, pore expansion, seborrheic dermatitis etc.

In this description, disclosed sebum preparation composition for external use is by promoting the differentiation of adipose cell, volume and the elasticity that can promote face and body.In face and body, requiring to promote volume and elastic position, while being coated with in this description disclosed Dermatologic preparation composition, volume and elasticity are promoted.The position of wanting cube and elastic enhancement can be the position of caving in due to aging.For example, can enumerate meat due to the aging cheek caved in etc.In being coated with this description, the cheek of depression during disclosed Dermatologic preparation composition, by promoting Adipocyte Differentiation, can promote volume.In addition, as other the example that requires to promote volume and elastic position, can enumerate breast and buttocks.In breast and buttocks are coated with this description, during disclosed Dermatologic preparation composition, breast and buttocks have elasticity, can obtain the effect that becomes plentiful.

In addition, in this description, disclosed Dermatologic preparation composition can improve the lines of face and body.The flower extract of the Radix Ginseng contained as effective ingredient or the seed extract of Radix Ginseng are by promoting the differentiation of adipose cell, promote volume and the elasticity of face and body, promote the biosynthesis of collagen, and, by suppressing the decomposition of collagen, can improve the lines due to the former thereby face and body that is damaged such as aging.

The dosage form of cosmetics involved in the present invention contains medium or the base material allowed on cosmetic conduct and learning or Dermatology.It can provide with all dosage forms that are suitable for topical application, for example, can be as solution, gel, solid, cream stick with paste anhydrous product, oil phase is dispersed in water and the Emulsion obtained, suspension, microemulsion, microcapsule, molecule ball or, the form of the folliculus dispersant of ion-type (liposome) and nonionic, or emulsifiable paste, toner, emulsion (lotion), powder, ointment, spraying or hide the form of flaw rod.These compositionss can be produced by the usual way in this field.In addition, compositions involved in the present invention can also be used with the form of foam (foam) or with the form of the aerosol composition of the propellant that further contains compression.

Cosmetics of the present invention can contain fatty material, organic solvent, lytic agent, concentrating agents, gellant, softening agent, antioxidant, suspending agent, stabilizing agent, foaming agent, aromatic, surfactant, water, ion-type or nonionic emulsifier, filler, the metal ion sealer, chelating agen, antiseptic, vitamin, blocker, wetting agent, quintessence oil, dyestuff, pigment, hydrophilic or lipophile activating agent, lipid vesicle, the perhaps normally used adjuvant in cosmetic conduct and learning or Dermatology field as normally used other composition arbitrarily in cosmetics.Above-mentioned adjuvant can be imported into the general amount of using in cosmetic conduct and learning or Dermatology field.

The skin preparations for extenal use of the seed extract of the flower extract that contains Radix Ginseng of the present invention or Radix Ginseng is for its dosage form, there is no particular limitation, for example, can carry out dosage form with the form of the cosmetics such as soft astringent, astringent lotion, nutrition astringent, nourishing cream, massage cream, essence, eye cream, eye essence, cleansing cream, cleaning foam, clean water, facial film, powder, skin lotion, emollient cream, soothing oil (body oil) and skin moistening essence.

Dermatologic preparation composition involved in the present invention can be the compositions on pharmaceutics.Compositions on above-mentioned pharmaceutics can also contain antiseptic, stabilizing agent, hydrating agents (hydrating agent) or emulsifying promoter, for the adjuvant on the pharmaceuticss such as the salt of regulating osmotic pressure and/or buffer agent and other to treating useful material.Compositions on above-mentioned pharmaceutics can be turned to emulsion, emulsifiable paste, ointment or gel etc. by dosage form.

Compositions on above-mentioned pharmaceutics is preferably by percutaneous dosing.

As the dosage of the seed extract of the flower extract of the Radix Ginseng of the effective ingredient of the compositions on above-mentioned pharmaceutics or Radix Ginseng, can change according to seriousness, administration path and the prescriber's of the specific disease of age, sex, body weight and the treatment of the object of receiving treatment or pathological state, disease or pathological state judgement.The decision of the dosage of the factor based on such is in those skilled in the art's level.General dosage is the 0.0001mg/kg/ days extremely roughly scopes of 2000g/kg/ days.

Embodiment

Below enumerate embodiment and experimental example, formation of the present invention and effect are carried out to more specific description.In addition, these experimental examples and embodiment are only the examples provided about the purpose of the example of understanding of the present invention as contributing to, and category of the present invention and scope are not restricted to following embodiment.

The preparation of the flower extract of [embodiment 1] Radix Ginseng

By 70%(v/v) ethanol 10L puts into the colored 1kg of dry Radix Ginseng, places at normal temperatures 1 day, extracted.Repeat identical operation 2 times, obtain extracting solution, by after the extracting liquid filtering obtained, with vacuum decker, its filtrate is concentrated into to 2L.Then, add the ether of same amount, remove the ether layer from filtrate, thereby remove chlorophyll.After repeating identical operation 3 times, with vacuum decker, that residual extracting solution is concentrated, obtain dry thing 150g.

The preparation of the seed oil of [embodiment 2] Radix Ginseng

After the seed 1kg of dry Radix Ginseng is pulverized, add hexane 10L, place at normal temperatures 1 day, extracted.Repeat identical operation 3 times, obtain extracting solution, by after extracting liquid filtering, with vacuum decker, its filtrate is concentrated, obtain the seed oil 120g of Radix Ginseng.Add sodium hydroxide in the extract oil obtained like this, remove free fatty, after being washed 3 times with distilled water, after using carbon to be decoloured, utilize inertness gas under the vacuum state of 100 ℃, remove odor pollutant, obtain the seed oil 100g of Radix Ginseng.

The preparation of the seed slag extract of [embodiment 3] Radix Ginseng

After the seed 1kg of dry Radix Ginseng is pulverized, add hexane 10L, place at normal temperatures 1 day, extracted.Repeat identical operation 3 times, from the seed of Radix Ginseng, except deoiling, having removed oil, in the seed slag of residual Radix Ginseng, add water 10L, place at normal temperatures 1 day, extracted.Repeat identical operation 3 times, obtain extracting solution, by after extracting liquid filtering, with vacuum decker, that its filtrate is concentrated, obtain dry thing 100g.

[experimental example 1] active oxygen generates and suppresses experiment

By relatively irradiating because of ultraviolet (UV) experiment of the ability of removing from the active oxygen of cell generation, the antioxidant effect of the flower extract of research Radix Ginseng and the seed extract of Radix Ginseng.

The vitamin C (Sigma Co. company system) that use is usually used when comparing antioxidant effect is as the positive controls of the flower extract of Radix Ginseng.In addition, the group that use is not processed substances as a control group.People HaCaT keratinocyte tomography cultivating system (human HaCaT keratinocytes monolayer culture system:P Boukamp, German Cancer Research Center, Heidelberg.Germany) is carried out to ultraviolet (UVB30mJ/cm ²) after processing, use according to extract 10ppm, 50ppm, the 100pmm of embodiment 1 preparation and processed respectively.After this, measure DCFH-DA(2 ', 7 '-dichloro-dihydro fluorescein(e) diacetate, 2 ' with fluorescence analyser (fluorescence plate reader), 7 '-dichlorodihydro-fluorescein diacetate, Molecular Probes, Inc).The matched group that does not carry out UV treatment of take is 100, with the ratio with respect to this, calculates the ROS(active oxygen) ratio that generates.Its result is illustrated in table 1 and Fig. 1.

Table 1

Substances	ROS production rate (%)
		Without UVB	100
UVB	161
		UVB+ embodiment 1(10ppm)	78
UVB+ embodiment 1(50ppm)	72
		UVB+ embodiment 1(100ppm)	70
UVB+ vitamin C 100 μ M	72

The watermiscible vitamin E (trolox) (Aldrich Co. company system) that use is usually used when comparing antioxidant effect is as the positive controls of the seed extract of Radix Ginseng.In addition, the group that use is not processed substances as a control group.People HaCaT keratinocyte tomography cultivating system (human HaCaT keratinocytes monolayer culture system:P Boukamp, German Cancer Research Center, Heidelberg.Germany) is carried out to ultraviolet (UVB30mJ/cm ²) after processing, be described in table 2 below the seed slag extract of processing respectively according to the Radix Ginseng of the seed oil of the Radix Ginseng of embodiment 2 preparations and embodiment 3.After this, with fluorescence analyser, measure DCFH-DA(2 ', 7 '-dichlorodihydro-fluorescein diacetate, Molecular Probes, Inc).Calculate ROS with the ratio of the matched group with respect to having carried out UV treatment and generate the ratio suppressed.Its result is illustrated in following table 2.

Table 2

Substances	ROS generates the ratio (%) suppressed
		Matched group	0
Watermiscible vitamin E 0.1mM	42.7
		Embodiment 2(10ppm)	52.9
Embodiment 2(1ppm)	38.3
		Embodiment 3(10ppm)	58.1
Embodiment 3(1ppm)	45.9

According to the flower extract of the Radix Ginseng of above-described embodiment 1 preparation, according to the seed slag extract of the Radix Ginseng of the seed oil of the Radix Ginseng of embodiment 2 preparations and embodiment 3, compare with matched group in people HaCaT keratinocyte tomography cultivating system and demonstrate the effect of effectively removing the active oxygen generated by UV.This is and the vitamin C of the activity index that is used as antioxidant or the good activity of the roughly the same degree of watermiscible vitamin E.; according to the flower extract of the Radix Ginseng of embodiments of the invention 1 preparation, according to the seed slag extract of the Radix Ginseng of the seed oil of the Radix Ginseng of embodiment 2 preparations and embodiment 3 by effectively removing the active oxygen of the reason that becomes skin injury; thereby protection comprises the skin of scalp and hair; and then confirmation can have the effect of generation, elastic reduction and the pigementation etc. of prevention wrinkle.

[experimental example 2] is for by single cell gel electrophoresis (Single cell gel electrophoresis; SCGE, Comet assay) the inhibition activity of the DNA oxidative damage that causes

After people's normal fibroblast (self once cultivates) is cultivated to 24 hours, by the flower extract of the Radix Ginseng of embodiment 1, according to seed slag extract and the vitamin E of the Radix Ginseng of the seed oil of the Radix Ginseng of embodiment 2 preparation and embodiment 3, be processed into respectively different concentration, after 12 hours, process the H as positive controls ₂o ₂after (ultimate density 10～3M), the result with fluorescence microscope with electrophoresis method dyeing.Now, be used as the KOMET3.1(Kinetic Imaging of image dissector (image analyzer), England), 25 cells are analyzed respectively to shown long (tail length) (the μ m) of tail of result cut off by DNA, its result is illustrated in following table 3 and Fig. 2.

Table 3

In the result of above-mentioned table 3, by the cell gel electrophoresis, the seed slag extract of the seed oil of the flower extract of the Radix Ginseng of embodiment 1 preparation, the Radix Ginseng of embodiment 2, the Radix Ginseng of embodiment 3 demonstrates the inhibition activity of effective oxidative dna damage, can confirm thus, the flower extract of Radix Ginseng involved in the present invention or the seed extract of Radix Ginseng play a role as the DNA oxidative damage inhibitor of the DNA single chain damage (single strand break) that suppresses to be caused by hydroxy radical (.OH).This is the important protection effect of gene of the cell of the protection skin that comprises scalp or hair.

[experimental example 3] I type procollagen generates analyzes (Type I Procollagen assay)

With 10 ⁵after individual concentration utilizes the 12 dull and stereotyped incubators in holes to cultivate human fibroblasts, the seed oil of the culture medium of flower extract 1ppm, the 10ppm that replaces to the Radix Ginseng that comprises embodiment 1 as shown in table 4,50ppm, the Radix Ginseng that comprises embodiment 2 or the seed slag extract 1ppm of the Radix Ginseng of embodiment 3, the culture medium of 10ppm.The 3rd day harvesting after cultivation, carry out quantitatively the amount of the I type procollagen with the generation of ELISA method.Use TGF-β (people's transforming growth factor-beta 1, Human Transforming Growth Factor-β 1, Roche Co.) as positive controls.Consequently, be made as 0, positive controls is made as to 100 is measured and the fiducial value of the value that obtains is calculated as the matched group of the seed extract of the flower extract with not comprising Radix Ginseng and Radix Ginseng.Result is illustrated in table 4 and Fig. 3.

Table 4

?	I type procollagen biosynthesis (%)
		Negative control group	0
Embodiment 1(1ppm)	7
		Embodiment 2(1ppm)	19
Embodiment 3(1ppm)	11
		Embodiment 1(10ppm)	22
Embodiment 2(10ppm)	39
		Embodiment 3(10ppm)	25
Embodiment 1(50ppm)	31
		TGF-β10ng/ml	100

In people's normal fibroblast tomography cultivating system (Normal human fibroblast monolayer culture system), the seed slag extract of the seed oil of the Radix Ginseng of the flower extract of the Radix Ginseng of embodiment 1, embodiment 2 and the Radix Ginseng of embodiment 3 is compared with matched group and is demonstrated clearly the effect that promotes that I type procollagen generates.That is, the Dermatologic preparation composition of the involved in the present invention flower extract that contains Radix Ginseng or the seed extract of Radix Ginseng, by promoting collagenation, can alleviate the collagen minimizing phenomenon by the aging generation of application on human skin.

[experimental example 4] MMP-1 expression inhibiting is analyzed

With 10 ⁵after individual concentration utilizes the dull and stereotyped incubator in 12 holes to cultivate human fibroblasts, with 40mJ/cm ²after irradiation ultraviolet radiation B, replace to flower extract 1ppm, the 10ppm of the Radix Ginseng that comprises embodiment 1 and the culture medium of 50ppm.The 2nd day harvesting after cultivation, to the MMP-1(matrix metalloproteinase I generated by the ELISA method) amount carry out quantitatively.Consequently, as with will be not containing substances, the matched group of irradiation ultraviolet radiation is not made as 100 and the fiducial value of measuring the value obtained is calculated, positive controls has been used the RA(tretinoin, Retinoic acid, Sigma.Co.).Result is illustrated in table 5 and Fig. 4.

Table 5

?	MMP-1 biosynthesis (%)
		Without UV	100
UV	152
		UV+ embodiment 1(1ppm)	128
UV+ embodiment 1(10ppm)	105
		UV+ embodiment 1(50ppm)	92
UV+RA10μM	45

In people's normal fibroblast tomography cultivating system, the flower extract of the Radix Ginseng of embodiments of the invention 1 has suppressed significantly by UVB40mJ/cm ²the expression of the MMP-1 induced.Therefore, can find out, the biosynthesis of the MMP-1 of the skin histology catabolic enzyme that the flower extract of the Radix Ginseng of embodiments of the invention 1 produces as the aging or external environmental factors because of inherence by obstruction, can suppress the minimizing of collagen in skin, improves wrinkle.

[experimental example 5] be take the wrinkle of skin that application on human skin is object and improved effect

Subjects 120 people wrinkly on the face for 30～50 years old, preparation have the seed oil of the nourishing cream (embodiment 4) of flower extract of the Radix Ginseng that contains embodiment 1 of the composition that following table 6 means and the Radix Ginseng that contains embodiment 2 and embodiment 3 Radix Ginseng seed slag extract nourishing cream (embodiment 5) and after the nourishing cream of compositions (comparative example 1) identical with above-described embodiment 4,5 nourishing cream containing the seed extract of the flower extract of Radix Ginseng and Radix Ginseng not, their wrinkle of skin of comparative evaluation improves effect (unit: % by weight).

Table 6

The experimenter uses respectively the nourishing cream of 3 months embodiment 4 or 5 at the left part of face, use the nourishing cream of 3 months comparative examples 1 at right part.After the left and right two-part skin condition of having measured in advance the face before the nourishing cream used, the nourishing cream of use is after 3 months, then measures same area, the variation of mensuration wrinkle of skin.In 24 ℃, the constant temperature of relative humidity 40%, humidity room, utilize die (replica) to copy the wrinkle at tail of the eye position, with visibility observation system (Visiometer system; C+K company system) measure wrinkle of skin.Calculate the variable quantity of wrinkle of skin by following mathematical expression 1.

Mathematical expression 1

Variable quantity (Δ %)=[(Tdi-Tdo)/Tdo] * 100

In above-mentioned formula 1, " Tdi " is the value at the mensuration position of D90, and " Tdo " is the value at the mensuration position of D0.

Utilize above-mentioned formula 1 to calculate result, consequently, with respect to the wrinkle of skin at the position of having used comparative example 1 be shown as 6.1 ± 7%(average ± standard deviation) the minimizing value, used the wrinkle of skin at the position of embodiment 4 to be shown as 35 ± 8% minimizing value, used the wrinkle of skin at the position of embodiment 5 to be shown as 32 ± 5% minimizing value, demonstrated good wrinkle of skin and improve effect.

[experimental example 6] improves the experiment of effect to the skin elasticity of application on human skin

The embodiment 4,5 that mensuration utilizes the composition of above-mentioned table 6 to prepare and the skin elasticity of comparative example 1 are improved effect.Using women 60 people more than 30 years old as object and be divided into 3 groups, object for each group, by above-described embodiment 4 or 5 and the nourishing cream of comparative example 1 be coated with every day 2 times on the face, after 12 weeks, utilize skin elasticity determinator (Cutometer SEM575, C+K Electronic Co. company system, Germany), measure skin elasticity.Calculated this result, consequently, with respect to the elastic effect at the position of having used comparative example 1 be shown as 0.21 ± 0.14(average ± standard deviation), used the elastic effect at the position of embodiment 4 to be shown as 0.30 ± 0.10, used the elastic effect at the position of embodiment 5 to be shown as 0.29 ± 0.08, demonstrated good skin elasticity and improve effect.The above results value refers to the character of the skin viscoelasticity (viscoelasticity) of skin elasticity determinator.

From the above results, can find out, in the situation that used the nourishing cream of embodiment 4 of the flower extract of the Radix Ginseng that contains embodiment 1, the skin elasticity that demonstrates 140% left and right is promoted effect.

[experimental example 7] utilized the chromatophorous melanin of Mus to generate inhibition

For the melanin of the seed slag extract of the Radix Ginseng of the seed oil of the Radix Ginseng of the flower extract of the Radix Ginseng of investigating embodiment 1 and embodiment 2 and embodiment 3 generates inhibition, utilized the pigment cell of Mus.

At first, at DMEM(Dulbeccos modified Eagles media) in added in the culture medium of the 2-O-myristoyl phorbol (tetradecanoylphorbol) of 10% hyclone, 100nM-13-acetate and 1nM cholera toxin (cholera toxin), at 37 ℃, 5%CO ₂condition under cultivate the Mus pigment cell (Mel-Ab cell) (Dooley, T.P.et al, Skin pharmacol, 7, pp188-200) from the C57BL/6 mice.Utilize 0.25% trypsin-EDTA by the Mel-Ab cell separation of cultivating, with 10 ⁵after the concentration of cells/well (cells/well) has been cultivated cell on 24 orifice plates, from second day, start to add respectively for three days on end the flower extract (embodiment 1) of the Radix Ginseng of 10ppm, 50ppm and 100ppm, the seed oil (embodiment 2) of Radix Ginseng and seed slag extract (embodiment 3) the cultivation of Radix Ginseng.Now, use arbutin (Sigma Co. company system) as positive controls.Then, remove culture fluid, after being washed with PBS, with 1N dissolution of sodium hydroxide cell, measure absorbance under 400nm after, by following mathematical expression 2, calculate melanin generating suppression, its result is illustrated in (method of Dooley) in following table 7, Fig. 5 and Fig. 6.

Mathematical expression 2

The absorbance of the absorbance/matched group of melanin generating suppression (%)=each substances of 100-[() * 100]

Table 7

As shown in Table 7 above, the seed slag extract of confirming the Radix Ginseng of the seed oil of Radix Ginseng of flower extract, embodiment 2 of the Radix Ginseng of embodiments of the invention 1 and embodiment 3 demonstrates the melanin generating suppression with the basic identical degree of arbutin.The seed slag extract that can confirm thus the Radix Ginseng of the seed oil of Radix Ginseng of flower extract, embodiment 2 of the Radix Ginseng of embodiments of the invention 1 and embodiment 3 demonstrates good whitening effect.

[experimental example 8] whitening effect to application on human skin

For the whitening effect to application on human skin of the seed slag extract of the Radix Ginseng of the seed oil of the Radix Ginseng of the flower extract of the Radix Ginseng of investigating embodiment 1, embodiment 2 and embodiment 3, implemented following experiment.

At first, 12 male of health of take are object, after having adhered at experimenter's upper arm position the opaque adhesive tape that has the hole that diameter is 1.5cm, irradiate the ultraviolet (UVB) of 1.5～2 times of left and right of each experimenter's minimal erythema dose, have induced the melanism of skin.

After above-mentioned ultraviolet irradiation, be coated with respectively seed slag extract and the hydroquinone of Radix Ginseng of seed oil, the embodiment 3 of the Radix Ginseng of flower extract as the Radix Ginseng of the embodiment 1 of substances, embodiment 2,1 place is not coated with whatever, observes the change of state of 10 time-of-weeks.Take one week as unit, by the Minolta company system of colorimeter CR2002(Japan) measured the color of skin.

Afterwards, utilize following mathematical expression 3 to calculate the coating time started point of above-mentioned each substances and poor (the Δ L*) of the colour of skin of coating deadline point, its result is illustrated in following table 8.In addition, the Δ L* of whitening effect by sample coating position and matched group position relatively judged, when Δ L* is 2 left and right, can judge that the whiteningization of pigment of precipitation is obvious; Be 1.5 when above, can judge and there is whitening effect.

Mathematical expression 3

The L* value of Δ L*=coating deadline point-the be coated with L* value of time started point

Table 8

Substances	The brightness of the colour of skin (Δ L*)
		Embodiment 1	1.91±0.17
Embodiment 2	1.94±0.11
		Embodiment 3	1.91±0.14
Hydroquinone (positive controls)	1.89±0.15
		Solvent (vehicle) (negative control group)	0.51±0.13

As shown in Table 8 above, the seed slag extract of confirming the Radix Ginseng of the seed oil of Radix Ginseng of flower extract, embodiment 2 of the Radix Ginseng of embodiments of the invention 1 and embodiment 3 means the brightness with the colour of skin of the basic identical degree of hydroquinone.This is that the flower extract of above-mentioned Radix Ginseng, the seed oil of Radix Ginseng and the seed slag extract of Radix Ginseng improve the pigementation generated by ultraviolet, the cause that the colour of skin is brightened.

The induction of [experimental example 9] people's keratinocyte

Measure the CE(Cornified Envelop of the differentiation phase generation of keratinocyte and human skin cell's strain (HaCaT), hornification coating) amount, the cell differentiation facilitation effect of the seed oil of the flower extract of the Radix Ginseng of research embodiments of the invention 1, the Radix Ginseng of embodiment 2 and the seed slag extract of embodiment 3.

Cultivating with the keratinocyte that adds the people who cultivates for 1 time in flask, and, after making it be attached to bottom, the substances shown in following table 9 is being added in culture fluid according to variable concentrations, cultivating 5 days, until cell is cultured to 70～80% left and right of floor space.Gather in the crops this cell, with PBS(phosphate buffered saline, phosphate buffered solution) after the washing, add and contain 2%SDS(Sodium Dodecyl Sulfate, sodium lauryl sulphate) and the DTT(Dithiothreitol of 20mM concentration, the Tri(Hydroxymethyl) Amino Methane Hydrochloride of 10mM concentration dithiothreitol, DTT) (Tris-HCl, pH7.4) 1ml, and implement ultrasonic Treatment, boiling (boiling), centrifugalize, be precipitated thing, make again this precipitate be suspended in PBS1ml, be determined at the absorbance under 340nm.In addition, take out the solution after a part of above-mentioned ultrasonic Treatment, measure the content of protein, when the evaluation of cell differentiation as benchmark.Using respectively and hang down calcium (0.03mM) processed group and high calcium (1.2mM) processed group as feminine gender/positive controls, add substances and during the result of the test implemented is illustrated in following table 9 in low calcium concentration.

Table 9

From the result of above-mentioned table 9, can confirm, the seed slag extract of the seed oil of the Radix Ginseng of the flower extract of the Radix Ginseng of embodiment 1, embodiment 2 and the Radix Ginseng of embodiment 3 promotes differentiation in keratinocyte.This means, make coming off and the turn around time normalization of the generating period of new horn cell of old horn cell, can strengthen skin moisture-keeping.In addition, flower extract, the seed oil of Radix Ginseng and the seed slag extract of Radix Ginseng of expression Radix Ginseng can promote removing of skin keratin.

The expression effect of the transglutaminase (transglutaminase) of [experimental example 10] people's keratinocyte

Put into people's keratinocyte 5 * 10 in each hole of 96 pass Tissue Culture Plates ⁴individual, adhere to 24 hours.After the Skin Cell strain of adhering to is carried out to the substances processing, through two days later, remove culture medium, preserve in the refrigerator-freezer of-20 ℃.By freeze-thaw repetitive operation 2 times, after making to have carried out the cytoclasis of mass treatment, be used in the acetone of preserving under-20 ℃: ethanol (1:1, v/v) is processed, and under 4 ℃, places 30 minutes, and cell is fixed.Afterwards, at room temperature place, make organic solvent evaporation, sealed (1% bovine serum albumin), transglutaminase antibody (primary antibody), the anti-mouse antibodies of HRP (secondary antibody) processing, add OPD(o-phennyldiamine, o-phenylenediamine) developed the color.Expression obtains by measure absorbance under 490nm, revises by measure background under 630nm and is undertaken.Respectively with hang down calcium (0.03mM) processed group and high calcium (1.2mM) processed group negative/positive controls, add flower extract 1ppm, 10ppm, the 100ppm of the Radix Ginseng of embodiment 1 and during the result of the test implemented is illustrated in following table 10 in low calcium concentration.

Table 10

In the result of above-mentioned table 10, with matched group, to compare, the flower extract of the Radix Ginseng of embodiments of the invention 1 demonstrates good expression.This shows, the flower extract of the Radix Ginseng of embodiments of the invention 1 improves the expression of transglutaminase.

The increase of the skin moisture-keeping ability of [experimental example 11] application on human skin

Adult men and women 75 people of more than 50～60 year old that will have xeroderma are divided into 3 groups, and the people of each group is coated with to the nourishing cream of embodiment 4, embodiment 5 and comparative example 1 on the face, and altogether be coated with 4 week 2 every day.Before coating starts and after coating through the time point of 1 week, 2 weeks, 4 weeks and end coating and again after 2 weeks (altogether through 6 weeks), measure the skin beauty water component with moisture tester under constant temperature, constant humidity condition (24 ℃, relative humidity 40%), its result is illustrated in following table 11.It is benchmark that result of the test be take the value of testing the moisture tester that just starts front mensuration, means to process the increment rate of the measured value after certain hour with percentage rate.

Table 11

Moisture tester is the moisture of skin determinator that the electrical conductivity by measuring epidermis is measured the water quantities be present in skin.As shown in above-mentioned table 11, be coated with the group of material of embodiment 5 of seed slag extract of the Radix Ginseng of the embodiment 4 of the flower extract of the Radix Ginseng that contains embodiment 1, the seed oil of Radix Ginseng that contains embodiment 2 and embodiment 3, with the matched group of the material that has been coated with comparative example 1, compare, the skin beauty water component further increases.In addition, the coating of the nourishing cream of the example of discontinuing the operation 4 and embodiment 5, after 2 weeks, (altogether through 6 weeks) measure moisture of skin and the numerical value that obtains is shown as and the essentially identical numerical value of the numerical value after 1～2 week again, hence one can see that, even be not coated with the material of embodiment 4 or embodiment 5, the moisture of skin that also can within prescribed period of time, keep constantly the coating by the seed slag extract of the seed oil of the flower extract of Radix Ginseng of the present invention, Radix Ginseng or Radix Ginseng to bring.

[experimental example 12] sebum secretion inhibition

In order to measure the effect that alleviates of oily skin, on the oily skin women of 20～45 years old 30 people's forehead part, to the flower extract that wherein sooner or later twice coating in a day of 10 people are used embodiment 4(to contain Radix Ginseng) frost, the seed oil that uses embodiment 5(to contain Radix Ginseng to sooner or later twice coating in a day of other 10 people and the seed slag extract of Radix Ginseng) frost, in addition, after remaining other 10 people twice coating in a day sooner or later used to the frost of comparative example 1, at 1 week, 2 weeks, 3 weeks, and after 4 weeks, utilize skin sebum composition test instrument (Sebumeter SM810), measure the sebum secretion amount of skin.Experiment is carried out under 20 ℃, the condition of relative humidity 20%, and following table 12 means its measurement results.Result means with experimenter 10 people's meansigma methods.In the situation that oily skin, measured value is more than 220, in the situation that normal skin is between 100～220.

Table 12

Substances	Before use	1 week	2 weeks	3 weeks	4 weeks
						Comparative example 1	246	240	239	235	230
Embodiment 4	248	237	232	216	211
						Embodiment 5	244	235	231	217	211

In the result of above-mentioned table 12, the nourishing cream of the embodiment 5 of the embodiment 4 of the flower extract of the Radix Ginseng that contains embodiments of the invention 1 and the seed extract that contains Radix Ginseng demonstrates the effect of the sebum secretion that suppresses excessive, can confirm to have the sebum regulating effect.Also can prevent thus the acne or the problem that are caused by the excessive sebum produced in comprising the skin of scalp.

[experimental example 13] by LPS(Lipopolysaccharide, lipopolysaccharide) the biosynthesis inhibition of the COX-2 (COX-2) of inducing

With 10 ⁵after individual concentration utilizes the dull and stereotyped incubator in 12 holes to cultivate human fibroblasts, after implementing LPS and processing, replace with the seed slag extract 1ppm of the Radix Ginseng of the culture medium of flower extract 1ppm, the 10ppm of the Radix Ginseng that comprises embodiment 1 and 100ppm, the seed oil of Radix Ginseng that comprises embodiment 2 and embodiment 3, the culture medium of 50ppm.The 2nd day harvesting after cultivation, carry out quantitatively the amount of the COX-2 (cyclooxygenase-2, COX-2) with the generation of Western blotting (Westren blot) method.Take matched group as 100, will be illustrated in following table 13 with the fiducial value of the value of utilizing densitometer (densitometer) to measure.The untreated fish group that the processing of the seed slag extract of the seed oil of the flower extract that now, matched group is the Radix Ginseng that do not carry out embodiment 1, the Radix Ginseng of embodiment 2 and the Radix Ginseng of embodiment 3 is cultivated.

Table 13

From the result of above-mentioned table 13, can find out, the seed slag extract of the Radix Ginseng of the seed oil of the Radix Ginseng of the flower extract of the Radix Ginseng of embodiments of the invention 1, embodiment 2 and embodiment 3, the biosynthesis that makes the COX-2 of being induced by the LPS as proinflammatory cytokine reduces in the mode of concentration dependent, the inflammation-inhibiting reaction.In addition, demonstrate thus the function that can alleviate skin problem.

[experimental example 14] acne and skin problem improve effect

The participant of experiment, for usually to comprise serious women 15 people of skin problem of acne, soap face in thermostatic constant wet chamber after, adapts to 30 minutes, relatively the number of acne and with the number of inflammation, the degree of mensuration skin problem.

For above-mentioned experimenter, after using respectively the nourishing cream of 4 weeks embodiment 4, embodiment 5 and comparative example 1, the degree of acne and skin problem relatively, its result is illustrated in following table 14.

Table 14

?	Embodiment 4(5 people)	Embodiment 5(5 people)	Comparative example 1(5 people)
				Skin problem improves effect	86%	84%	32%

From the result of above-mentioned table 14, can find out, the embodiment 5 coatings result of 4 weeks of seed slag extract of Radix Ginseng of embodiment 4, the seed oil of Radix Ginseng that contains embodiment 2 and embodiment 3 of flower extract that will contain the Radix Ginseng of embodiments of the invention 1 is, demonstrates the minimizing of existing acne number and with the effect of improving of the problem of inflammation.

The color of [experimental example 15] application on human skin (face) is improved effect

For the compositions of the flower extract that contains Radix Ginseng of the present invention, for the blood circulation facilitation effect of evaluating skin, utilize LDPI(Laser Doppler Perfusion Imager, the laser Doppler perfusion imaging instrument) measure the blood circulation of skin.LDPI is known as the sanguimotor equipment of measuring skin, being now still at the equipment used, is not only can measure for the speed of the blood of the blood capillary of skin and amount and can measure the very sensitive equipment of small artery and venular blood flow.

Constant temperature and humidity is indoor soap face after, adapt to 30 minutes, utilize LDPI and, as infrared (IR, Infrad) camera of skin temperature sensing equipment, measure initial value.The participant of experiment is the ice-cold women of usual trick 40 people, measures the initial stage blood flow of the latter half of forehead with LDPI, utilizes the IR camera to measure the bottom of eyes and the skin temperature initial value of cheek.

Use the frost of 1 week embodiment 4 and 5 for the experimenter after, relatively blood flow and skin temperature (℃), its result is illustrated respectively in following table 15.

Table 15

As mentioned above, used the frost of the embodiment 4 of the flower extract that contains Radix Ginseng of the present invention with the seed oil that contains Radix Ginseng and the result of the frost of the embodiment 5 of the seed slag extract that contains Radix Ginseng to be, from experimenter's face, can confirm, utilize peripheral blood circulation to improve effect, improved the color of experimenter's face, this teaching in the situation that finally contain the flower extract of Radix Ginseng of the present invention or the seed extract of Radix Ginseng, the nutritional labeling of skin can be effectively transmitted, skin aging can be contributed to suppress and postpone.

[embodiment 6,7 and 8, comparative example 2]

According to the composition of following table 16, the compositions of mix and prepare flower extract containing the Radix Ginseng of with good grounds above-described embodiment 1 preparation, according to the seed slag of the seed oil of the Radix Ginsengs of embodiment 2 and 3 preparations and Radix Ginseng, extracting.

Table 16

Composition (% by weight)	Embodiment 6	Embodiment 7	Embodiment 8	Comparative example 2
					The flower extract of Radix Ginseng (embodiment 1)	10	-	-	-
The seed oil of Radix Ginseng (embodiment 2)	-	10	-	-
					The seed slag extract (embodiment 3) of Radix Ginseng	-	-	0.1	-
Miglyol 812N	70	70	70	80
					Ethanol	10	10	10	10
Water	Surplus	Surplus	Surplus	Surplus

The test of [experimental example 16] anti-loss effect

Experimenter 30 people with initial stage alopecia phenomenon of take are object, have implemented about the experiment of anti-loss effect 16 weeks.The compositions of above-described embodiment 6,7,8 and comparative example 2 is used 1 time every day, used altogether 16 weeks, after 16 weeks, collect alopecia during hair washing, its radical of number, its result is illustrated in table 17.

Table 17

Processed group	The mean elements of alopecia (root)/standard deviation
		Comparative example 2	82.80/10.32
The flower extract of embodiment 6(Radix Ginseng)	75.07/8.70
		The seed oil of embodiment 7(Radix Ginseng)	76.27/9.10
The seed slag extract of embodiment 8(Radix Ginseng)	79.21/6.10

As shown in above-mentioned table 17, can confirm, in the situation that the compositions of the seed slag extract of the seed oil of the flower extract that use contains Radix Ginseng, Radix Ginseng or Radix Ginseng has carried out processing, with comparative example, compare, the radical of alopecia significantly reduces.

The experiment of [experimental example 17] dandruff fungi restraining effect

In order to investigate the flower extract of above-described embodiment 1(Radix Ginseng), the seed oil of embodiment 2(Radix Ginseng) and the seed slag extract of embodiment 3(Radix Ginseng) whether there is the effect that suppresses dandruff fungus (P.ovale ATCC12087), use fluid medium dilution method (broth dilution method) to be tested.Now, after carrying out doubling dilution, carry out standing cultivation after 24 hours and 48 hours under 32 ℃, according to the degree be suspended, judge that fungus has or not propagation, will not have the Cmin of the propagation of fungus to be defined as MIC(Minimum Inhibition Concentration, minimal inhibitory concentration).In addition, in the situation that mixed liquor opaque, be difficult to judge that fungus has or not propagation, by microscopic examination, confirmed.Its result is illustrated in following table 18.

Table 18

Processed group	The MIC(minimal inhibitory concentration)
		The flower extract of embodiment 1(Radix Ginseng)	0.05%
The seed oil of embodiment 2(Radix Ginseng)	0.04%
		The seed slag extract of embodiment 3(Radix Ginseng)	0.07%

From above-mentioned table 18, can find out, the minimizing degree of the MIC of the seed slag extract of the flower extract of Radix Ginseng, the seed oil of Radix Ginseng and Radix Ginseng is good.Therefore, the seed slag extract of the seed oil of the flower extract of Radix Ginseng, Radix Ginseng and Radix Ginseng can be effectively for treatment and the prevention of the dandruff.

[experimental example 18] hair gloss is promoted effect

With after shampoo 0.1g washing hair sample 0.1g, after cleaning with flowing water, under 25 ℃, 50% damp condition, make the hair samples dried 6 hours.Afterwards, be coated with respectively the embodiment 6,7,8 of above-mentioned table 16 and the compositions 0.1g of comparative example 2, by following method, use goniophotometer to measure gloss.

Measure the gloss of hair sample with the goniophotometer that Halogen light is installed.In above-mentioned goniophotometer, the angle of incidence of light is 30 degree, can obtain the value of the reflection of 0～90 degree.Glossiness is calculated by following mathematical expression 4.

Mathematical expression 4

Glossiness (G)=Sf/d

Sf: mean the highest value in regular reflection value, reflected value

D: mean diffuse-reflectance, the value when 0 spends

Diffuse-reflectance is fewer, and regular reflection is more, and gloss demonstrates good result, and its measurement result is illustrated in following table 19.

Table 19

Processed group	d	Sf	G
				Comparative example 2	6.49	68.41	10.54
The flower extract of embodiment 6(Radix Ginseng)	3.58	83.20	23.24
				The seed oil of embodiment 7(Radix Ginseng)	2.81	87.45	31.12
The seed slag extract of embodiment 8(Radix Ginseng)	3.91	64.23	16.43

From the result of above-mentioned table 19, can confirm, in the situation that the compositions of the seed extract of the flower extract that use contains Radix Ginseng or Radix Ginseng has carried out processing, with comparative example, compare, hair gloss significantly improves.

The confirmation of the differentiation facilitation effect of [test example 19] adipose cell

Buy people's bone marrow stroma stem cell (human bone marrow mesenchymal stem cells, Hbm-MSCs) from Lonza company (Lonza, Inc.Walkersbille, MD, USA), cultivated according to the description of Lonza company.Adipocyte Differentiation is except use troglitazone (troglitazone, TRO) replacement indomethacin (indomethacin) is induced the Adipocyte Differentiation of stroma stem cell, and the method for recommending according to Lonza company is carried out.Particularly, add insulin 1g/ml, dexamethasone (dexamethasone, DEXA) 1M, isobutyl methylxanthine (isobutylmethylxanthine, IBX) 0.5mM and TRO2M in the culture fluid of stroma stem cell, cultivated, make stroma stem cell be divided into adipose cell.In the situation that experimental group, to the inducing of Adipocyte Differentiation the time, using respectively seed slag extract, troglitazone and glibenclamide (Glibenclamide) (buying from Sigma-aldrich, the U.S.A) 10M of Radix Ginseng of seed oil, embodiment 3 of Radix Ginseng of flower extract, the embodiment 2 of the Radix Ginseng of embodiment 1 to process (processing 10 days with the intervals of two days), each 10M is added in culture medium.Induce the Adipocyte Differentiation of stroma stem cell after 10 days, remove cell culture fluid, with after phosphate buffered solution (phosphate buffered saline, PBS) washed cell, use 10% formalin/PBS solution 0.2ml/cm ², make cell fix 10 minutes.With 60% aqueous isopropanol 0.5ml/cm ²by the cell washing that is fixed 1 time, use the O by oil red O(Oil Red) be dissolved in the dyeing liquor formed in isopropyl alcohol, with 0.2ml/cm ²lipid in adipose cell is dyeed.After the oil red O stripping that makes to have dyeed with 100% aqueous isopropanol, with the wavelength of 500nm, measure absorbance.Its result is illustrated in following table 20.Each data value IDX(-) negative control group is revised.

Table 20

Processed group	The ratio of the dyeing that oil red is relative
		Comparative example	1.00
The flower extract of embodiment 1(Radix Ginseng)	2.18
		The seed oil of embodiment 2(Radix Ginseng)	2.74
The seed slag extract of embodiment 3(Radix Ginseng)	1.75

From above-mentioned table 20, can confirm, the flower extract of Radix Ginseng and the seed extract of Radix Ginseng promote the differentiation (P value<0.01) of adipose cell.

Below the dosage form example of the compositions that the present invention relates to is described, but be not limited thereto.

[dosage form example 1] soft astringent (skin lotion)

Table 21

Gradation composition	Content (% by weight)
		Purified Water	Surplus
The flower extract of Radix Ginseng or the seed extract of Radix Ginseng	0.1
		Butanediol	2.0
Propylene glycol	2.0
		CVP Carbopol ETD2050	0.1
The PEG-12 nonylplenyl ether	0.2
		Polysorbate 80	0.4
Ethanol	10.0
		Triethanolamine	0.1
Antiseptic, pigment, spice	In right amount

[dosage form example 2] nutrition astringent (milk skin lotion)

Table 22

[dosage form example 3] massage cream

Table 23

[dosage form example 4] facial film

Table 24

Gradation composition	Content (% by weight)
		Purified Water	Surplus
The flower extract of Radix Ginseng or the seed extract of Radix Ginseng	0.1
		Polyvinyl alcohol	13.0
Sanlose	0.2
		Glycerol	5.0
Allantoin	0.1
		Ethanol	6.0
The PEG-12 nonylplenyl ether	0.3
		Polysorbate 60	0.3
Antiseptic, pigment, spice	In right amount

Claims

1. the seed slag extract of Radix Ginseng is being manufactured for preventing or improve the purposes of the preparation composition for external use of inflammation.

2. purposes according to claim 1, wherein, described compositions further contains more than one in the seed oil of the flower extract of Radix Ginseng and Radix Ginseng.

3. purposes according to claim 1, wherein, the concentration that the gross weight that the seed slag extract of described Radix Ginseng be take with respect to Dermatologic preparation composition is 0.001～10 % by weight contains.

4. purposes according to claim 1, wherein, described ginseng seed's slag extract reduces the biosynthesis of COX-2.