CN103478410A - Probiotic protein feed produced through peltate yam rhizomes and production method thereof - Google Patents
Probiotic protein feed produced through peltate yam rhizomes and production method thereof Download PDFInfo
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Abstract
The invention discloses a method for producing probiotic protein feed through peltate yam rhizomes. The method comprises the following steps that (1) peltate yam rhizome cellulose and starch are separated; (2) the peltate yam rhizome cellulose is fermented; (3) the peltate yam rhizome starch is liquefied and saccharified; (4) the probiotic protein feed is prepared. Firstly, the starch and the cellulose in the peltate yam rhizomes are separated through a mechanical method, the cellulose is used for solid state fermentation to obtain fibrous residues containing cellulose and mycoprotein, then a suspension solution for separation of the starch is used for subsequent extraction of saponin, supernate is added to the peltate yam rhizome starch, and circulation utilization of waste water is achieved. Peltate yam rhizome starch milk is used for cultivating probiotics after being liquefied and saccharified, the probiotics include saccharomyces boulardii and lactobacillus acidophilus, then cellulose fermentation broth and probiotic culture are completely mixed to be uniform, and the probiotic protein feed containing the cellulose is obtained. Comprehensive utilization of the starch and the cellulose in the production process of the diosgenin saponin is completely achieved, production benefits are improved, and exhaust amount and pollution are reduced largely.
Description
Technical field
The present invention relates to a kind of probio protein feed and production method thereof, be specially a kind of probio protein feed and method thereof of utilizing yellow ginger to produce.
Background technology
Yellow ginger formal name used at school dioscorea zingiberensis wright (Dioscorea zingiberensis.C.H.Wright), have another name called the duration and degree of heating root, with root-like stock, is used as medicine, and is China's tradition parts of generic medicinal plants, and the title of " medicinal gold " is arranged.Contain abundant Dioscin in the yellow ginger root-like stock, its hydrolysate is that diosgenin is turmeric saponin, and the plant that saponin content is higher in the world is few, mainly is distributed in China and Mexico.At present, the saponin export volume of China is only second to Mexico, occupies the second in the world.
In yellow ginger except containing saponin(e, also containing the dioscorea zingiberensis pigment of the cellulose of the starch of 40%-50%, 40-50 and hemicellulose and lignin, 2% left and right, the organic matters such as 2% left and right plant essence, tannic acid.At present, yellow ginger is mainly used to produce saponin, but the yellow ginger traditional processing technology causes environmental pollution, a lot of documents all Alternative improve, mainly follow-up acid treatment consumption will be reduced after starch and fiber separation, thereby reduce to pollute, but Dioscorea. zingiberensis Wright Starch, cellulose etc. still could not find the suitable approach that utilizes.
Summary of the invention
The object of the present invention is to provide a kind of probio protein feed and method thereof of utilizing yellow ginger to produce, utilize the accessory substance starch of turmeric saponin production and cellulose to prepare the probio protein feed, realized the resource of yellow ginger accessory substance, improved economic benefit.
The present invention is achieved through the following technical solutions:
A kind of method of utilizing yellow ginger to produce the probio protein feed, comprise the steps,
1) yellow ginger cellulosic and starch separate: after yellow ginger is cleaned to pulverizing, filtration obtains cellulosic; To leach that thing is standing is divided into three layers, upper strata is supernatant, and centre is suspension, and lower floor is starch, pours out supernatant standby, isolates lower floor's starch washing;
2) yellow ginger cellulosic fermentation: add wheat bran, ammonium sulfate and Tween 80 in the yellow ginger cellulosic, and stir and obtain the cellulosic mixture, then sterilizing is cooled to 35-40 ℃ of access Trichoderma viride, after 27-33 ℃ of constant temperature culture 3-4d, obtain the yellow ginger fibrous residue of cellulase and mycoprotein;
3) Dioscorea. zingiberensis Wright Starch liquefying-saccharifying: the Dioscorea. zingiberensis Wright Starch after washing is added to supernatant, making solid content in starch milk is 8-12%, its pH value is adjusted to 6.2-6.5, and is warming up to 70-80 ℃, then add AMS and calcium chloride, and insulation enzymolysis 1-2h, then cool the temperature to 55-60 ℃, then the pH value is adjusted to 5.4-6.0, and add carbohydrase and Pullulanase, insulation enzymolysis 3-4h, obtain the Dioscorea. zingiberensis Wright Starch enzymolysis liquid;
4) probio protein feed preparation: after the Dioscorea. zingiberensis Wright Starch enzymolysis liquid is cooled to 45-50 ℃, add (NH
4)
2sO
4, peptone, anhydrous sodium acetate, diammonium hydrogen citrate, Tween 80, magnesium sulfate, manganese sulfate, dipotassium hydrogen phosphate, stir and regulate the pH value and obtain mixed enzymolysis liquid to 5.0-6.0, access again Paula enlightening yeast and lactobacillus acidophilus, 34-39 ℃ of bottom fermentation 48-60h, after the yellow ginger fibrous residue is added, mix, and low temperature drying obtains yellow ginger probio protein feed.
Further, in step 1), yellow ginger adopts the cadmium yellow ginger of water content 65-70%.
Further, in step 1), adopt 80-100 purpose filter-cloth filtering to obtain cellulosic, be divided into three layers after leaching the standing 5-6h of thing.
Further, step 2), in, add respectively in proportion wheat bran and the ammonium sulfate of 10-20g and the Tween 80 of 0.8-1.2ml of 20-25g in the yellow ginger cellulosic in every kilogram of yellow ginger cellulosic.
Further, step 2) in, sterilizing 20min-25min at the temperature of 121 ℃ after being cooled to 35 ℃-40 ℃, accesses the Trichoderma viride liquid spawn of 6-10ml in proportion in every 100mg cellulosic mixture.
Further, in step 3), after adding AMS and calcium chloride, the vigor of corresponding every gram starch AMS is 200-240U, the Ca in every kilogram of starch
2+ion concentration is 200-240mg.
Further, in step 3), after adding carbohydrase and Pullulanase, the vigor of corresponding every gram turmeric starch saccharification enzyme is 240-300U, and the vigor of Pullulanase is 20-40U.
Further, in step 4), add (the NH of 8-10g by every liter of Dioscorea. zingiberensis Wright Starch enzymolysis liquid
4)
2sO
4, the peptone of 4-5g, the anhydrous sodium acetate of 2-3.0g, the diammonium hydrogen citrate of 5-6g, the Tween 80 of 0.8-1.0mL, the magnesium sulfate of 0.2-0.3g, the manganese sulfate of 0.04-0.06g, the dipotassium hydrogen phosphate of 2.0-4.0g.
Further, in step 4), access respectively in proportion the enlightening yeast and lactobacillus acidophilus bacterial classification 6-8ml in the Paula of 4-6ml in every 100ml mixed enzymolysis liquid.
A kind of probio protein feed that utilizes yellow ginger to produce, made by above any one described method of utilizing yellow ginger to produce the probio protein feed.
Compared with prior art, the present invention has following useful technique effect:
A kind of method of utilizing yellow ginger to produce the probio protein feed of the present invention, at first separate the starch in yellow ginger and cellulosic by mechanical means, cellulosic obtained to the fibrous residue of cellulase and mycoprotein for solid state fermentation; Suspension during then by separating starch is for the extraction of follow-up saponin, supernatant is joined in Dioscorea. zingiberensis Wright Starch, realized recycling of waste water, the Dioscorea. zingiberensis Wright Starch breast through liquefaction, after saccharification for cultivating probio---Paula enlightening yeast and lactobacillus acidophilus fully mix by cellulose fermentation thing and probiotic bacterial cultures the probio protein feed that obtains cellulase subsequently.
Simultaneously owing to when saponin is extracted production, starch and cellulosic being separated, while therefore producing, acid hydrolysis volume and solid content greatly reduce, make sour use amount reduce 85-93%, hydrolysate is washed neutral water consumption and also is down to original 10-15%, the amount of gasoline that follow-up saponin is extracted also is down to original 10-20%, fully realize starch and cellulosic comprehensive utilization in the turmeric saponin production process, improved productivity effect, greatly reduced discharge capacity and pollution.
Further, the control by the vigor to adding enzyme in step and to the control of other admixture quality, and the control of technological parameter, improve the content of active principle in protein feed, guaranteed the quality of product.
A kind of probio protein feed that utilizes yellow ginger to produce of the present invention, be rich in Paula enlightening yeast, lactobacillus acidophilus, Trichoderma viride and cellulase, not only can be the useful animal health of livestock probiotic supplemented, and the cellulase in feed can also promote absorption and raising efficiency of feed utilization to nutriment.
The specific embodiment
Below in conjunction with specific embodiment, the present invention is described in further detail, and the explanation of the invention is not limited.
Example 1
A kind of method of utilizing yellow ginger to produce the probio protein feed, comprise the steps,
1) yellow ginger cellulosic and starch separate: after yellow ginger is cleaned to pulverizing, filtration obtains cellulosic; To leach that thing is standing is divided into three layers, upper strata is supernatant, and centre is suspension, and lower floor is starch, pours out supernatant standby, isolates lower floor's starch washing; Preferably, yellow ginger adopts the cadmium yellow ginger of water content 65%; Concrete employing 100 purpose filter-cloth filterings obtain cellulosic, are divided into three layers after leaching the standing 6h of thing.
2) yellow ginger cellulosic fermentation: add wheat bran, ammonium sulfate and Tween 80 in the yellow ginger cellulosic, and stir and obtain the cellulosic mixture, then sterilizing is cooled to 35 ℃ of access Trichoderma virides, after 30 ℃ of constant temperature culture 3d, obtains the yellow ginger fibrous residue of cellulase and mycoprotein; Concrete, add respectively in proportion wheat bran and the ammonium sulfate of 10g and the Tween 80 of 0.8ml of 20g in every kilogram of yellow ginger cellulosic in the yellow ginger cellulosic; Sterilizing 20min at the temperature of 121 ℃ after being cooled to 35 ℃, accesses the Trichoderma viride liquid spawn of 10ml in proportion in every 100mg cellulosic mixture.
3) Dioscorea. zingiberensis Wright Starch liquefying-saccharifying: the Dioscorea. zingiberensis Wright Starch after washing is added to supernatant, making solid content in starch milk is 8%, its pH value is adjusted to 6.2, and is warming up to 80 ℃, then add AMS and calcium chloride, and insulation enzymolysis 1.5h, then cool the temperature to 58 ℃, then the pH value is adjusted to 5.5, and add carbohydrase and Pullulanase, insulation enzymolysis 3h, obtain the Dioscorea. zingiberensis Wright Starch enzymolysis liquid; Concrete, after adding AMS and calcium chloride, the vigor of corresponding every gram starch AMS is 240U, the Ca in every kilogram of starch
2+ion concentration is 240mg; After adding carbohydrase and Pullulanase, the vigor of corresponding every gram turmeric starch saccharification enzyme is 300U, and the vigor of Pullulanase is 40U.
4) probio protein feed preparation: after the Dioscorea. zingiberensis Wright Starch enzymolysis liquid is cooled to 45 ℃, add (NH
4)
2sO
4, peptone, anhydrous sodium acetate, diammonium hydrogen citrate, Tween 80, magnesium sulfate, manganese sulfate, dipotassium hydrogen phosphate, stir and regulate pH value to 6.0 and obtain mixed enzymolysis liquid, access again Paula enlightening yeast and lactobacillus acidophilus, 37 ℃ of bottom fermentation 60h, after will the yellow ginger fibrous residue adding, mix, and low temperature drying obtains yellow ginger probio albumen and raises.Concrete, add (the NH of 10g by every liter of Dioscorea. zingiberensis Wright Starch enzymolysis liquid
4)
2sO
4, the peptone of 5.0g, the anhydrous sodium acetate of 3.0g, the diammonium hydrogen citrate of 6.0g, the Tween 80 of 1.0mL, the magnesium sulfate of 0.3g, the manganese sulfate of 0.06g, the dipotassium hydrogen phosphate of 4.0g; Access respectively in proportion the enlightening yeast and lactobacillus acidophilus bacterial classification 6ml in the Paula of 4ml in every 100ml mixed enzymolysis liquid.
Example 2
A kind of method of utilizing yellow ginger to produce the probio protein feed, comprise the steps,
1) yellow ginger cellulosic and starch separate: after yellow ginger is cleaned to pulverizing, filtration obtains cellulosic; To leach that thing is standing is divided into three layers, upper strata is supernatant, and centre is suspension, and lower floor is starch, pours out supernatant standby, isolates lower floor's starch washing; Preferably, yellow ginger adopts the cadmium yellow ginger of water content 66%; Concrete employing 80 purpose filter-cloth filterings obtain cellulosic, are divided into three layers after leaching the standing 5h of thing.
2) yellow ginger cellulosic fermentation: add wheat bran, ammonium sulfate and Tween 80 in the yellow ginger cellulosic, and stir and obtain the cellulosic mixture, then sterilizing is cooled to 35 ℃ of access Trichoderma virides, after 30 ℃ of constant temperature culture 4d, obtains the yellow ginger fibrous residue of cellulase and mycoprotein; Concrete, add respectively in proportion wheat bran and the ammonium sulfate of 20g and the Tween 80 of 1.2ml of 25g in every kilogram of yellow ginger cellulosic in the yellow ginger cellulosic; Sterilizing 20min at the temperature of 121 ℃ after being cooled to 35 ℃, accesses the Trichoderma viride liquid spawn of 10ml in proportion in every 100mg cellulosic mixture.
3) Dioscorea. zingiberensis Wright Starch liquefying-saccharifying: the Dioscorea. zingiberensis Wright Starch after washing is added to supernatant, making solid content in starch milk is 9%, its pH value is adjusted to 6.3, and is warming up to 75 ℃, then add AMS and calcium chloride, and insulation enzymolysis 2h, then cool the temperature to 60 ℃, then the pH value is adjusted to 5.8, and add carbohydrase and Pullulanase, insulation enzymolysis 4h, obtain the Dioscorea. zingiberensis Wright Starch enzymolysis liquid; Concrete, after adding AMS and calcium chloride, the vigor of corresponding every gram starch AMS is 220U, the Ca in every kilogram of starch
2+ion concentration is 220mg; After adding carbohydrase and Pullulanase, the vigor of corresponding every gram turmeric starch saccharification enzyme is 240U, and the vigor of Pullulanase is 20U.
4) probio protein feed preparation: after the Dioscorea. zingiberensis Wright Starch enzymolysis liquid is cooled to 45 ℃, add (NH
4)
2sO
4, peptone, anhydrous sodium acetate, diammonium hydrogen citrate, Tween 80, magnesium sulfate, manganese sulfate, dipotassium hydrogen phosphate, stir and regulate pH value to 5.5 and obtain mixed enzymolysis liquid, access again Paula enlightening yeast and lactobacillus acidophilus, 37 ℃ of bottom fermentation 48h, after will the yellow ginger fibrous residue adding, mix, and low temperature drying obtains yellow ginger probio albumen and raises.Concrete, add (the NH of 8.0g by every liter of Dioscorea. zingiberensis Wright Starch enzymolysis liquid
4)
2sO
4, the peptone of 4.0g, the anhydrous sodium acetate of 2.0g, the diammonium hydrogen citrate of 5.0g, the Tween 80 of 0.8mL, the magnesium sulfate of 0.2g, the manganese sulfate of 0.04g, the dipotassium hydrogen phosphate of 2.0g; Access respectively in proportion the enlightening yeast and lactobacillus acidophilus bacterial classification 8ml in the Paula of 6ml in every 100ml mixed enzymolysis liquid.
Example 3
A kind of method of utilizing yellow ginger to produce the probio protein feed, comprise the steps,
1) yellow ginger cellulosic and starch separate: after yellow ginger is cleaned to pulverizing, filtration obtains cellulosic; To leach that thing is standing is divided into three layers, upper strata is supernatant, and centre is suspension, and lower floor is starch, pours out supernatant standby, isolates lower floor's starch washing; Preferably, yellow ginger adopts the cadmium yellow ginger of water content 67%; Concrete employing 90 purpose filter-cloth filterings obtain cellulosic, are divided into three layers after leaching the standing 6h of thing.
2) yellow ginger cellulosic fermentation: add wheat bran, ammonium sulfate and Tween 80 in the yellow ginger cellulosic, and stir and obtain the cellulosic mixture, then sterilizing is cooled to 40 ℃ of access Trichoderma virides, after 33 ℃ of constant temperature culture 3d, obtains the yellow ginger fibrous residue of cellulase and mycoprotein; Concrete, add respectively in proportion wheat bran and the ammonium sulfate of 12g and the Tween 80 of 0.9ml of 21g in every kilogram of yellow ginger cellulosic in the yellow ginger cellulosic; Sterilizing 25min at the temperature of 121 ℃ after being cooled to 40 ℃, accesses the Trichoderma viride liquid spawn of 7ml in proportion in every 100mg cellulosic mixture.
3) Dioscorea. zingiberensis Wright Starch liquefying-saccharifying: the Dioscorea. zingiberensis Wright Starch after washing is added to supernatant, making solid content in starch milk is 10%, its pH value is adjusted to 6.4, and is warming up to 70 ℃, then add AMS and calcium chloride, and insulation enzymolysis 1h, then cool the temperature to 55 ℃, then the pH value is adjusted to 5.4, and add carbohydrase and Pullulanase, insulation enzymolysis 3.5h, obtain the Dioscorea. zingiberensis Wright Starch enzymolysis liquid; Concrete, after adding AMS and calcium chloride, the vigor of corresponding every gram starch AMS is 210U, the Ca in every kilogram of starch
2+ion concentration is 230mg; After adding carbohydrase and Pullulanase, the vigor of corresponding every gram turmeric starch saccharification enzyme is 250U, and the vigor of Pullulanase is 30U.
4) probio protein feed preparation: after the Dioscorea. zingiberensis Wright Starch enzymolysis liquid is cooled to 50 ℃, add (NH
4)
2sO
4, peptone, anhydrous sodium acetate, diammonium hydrogen citrate, Tween 80, magnesium sulfate, manganese sulfate, dipotassium hydrogen phosphate, stir and regulate pH value to 5.0 and obtain mixed enzymolysis liquid, access again Paula enlightening yeast and lactobacillus acidophilus, 34 ℃ of bottom fermentation 50h, after will the yellow ginger fibrous residue adding, mix, and low temperature drying obtains yellow ginger probio albumen and raises.Concrete, add (the NH of 9.0g by every liter of Dioscorea. zingiberensis Wright Starch enzymolysis liquid
4)
2sO
4, the peptone of 4.5g, the anhydrous sodium acetate of 2.5g, the diammonium hydrogen citrate of 5.5g, the Tween 80 of 0.9mL, the magnesium sulfate of 0.2g, the manganese sulfate of 0.04g, the dipotassium hydrogen phosphate of 2.0g; Access respectively in proportion the enlightening yeast and lactobacillus acidophilus bacterial classification 8ml in the Paula of 6ml in every 100ml mixed enzymolysis liquid.
Example 4
A kind of method of utilizing yellow ginger to produce the probio protein feed, comprise the steps,
1) yellow ginger cellulosic and starch separate: after yellow ginger is cleaned to pulverizing, filtration obtains cellulosic; To leach that thing is standing is divided into three layers, upper strata is supernatant, and centre is suspension, and lower floor is starch, pours out supernatant standby, isolates lower floor's starch washing; Preferably, yellow ginger adopts the cadmium yellow ginger of water content 68%; Concrete employing 85 purpose filter-cloth filterings obtain cellulosic, are divided into three layers after leaching the standing 5h of thing.
2) yellow ginger cellulosic fermentation: add wheat bran, ammonium sulfate and Tween 80 in the yellow ginger cellulosic, and stir and obtain the cellulosic mixture, then sterilizing is cooled to 36 ℃ of access Trichoderma virides, after 27 ℃ of constant temperature culture 4d, obtains the yellow ginger fibrous residue of cellulase and mycoprotein; Concrete, add respectively in proportion wheat bran and the ammonium sulfate of 14g and the Tween 80 of 1.0ml of 22g in every kilogram of yellow ginger cellulosic in the yellow ginger cellulosic; Sterilizing 21min at the temperature of 121 ℃ after being cooled to 36 ℃, accesses the Trichoderma viride liquid spawn of 6ml in proportion in every 100mg cellulosic mixture.
3) Dioscorea. zingiberensis Wright Starch liquefying-saccharifying: the Dioscorea. zingiberensis Wright Starch after washing is added to supernatant, making solid content in starch milk is 11%, its pH value is adjusted to 6.5, and is warming up to 72 ℃, then add AMS and calcium chloride, and insulation enzymolysis 1.8h, then cool the temperature to 56 ℃, then the pH value is adjusted to 6.0, and add carbohydrase and Pullulanase, insulation enzymolysis 3h, obtain the Dioscorea. zingiberensis Wright Starch enzymolysis liquid; Concrete, after adding AMS and calcium chloride, the vigor of corresponding every gram starch AMS is 240U, the Ca in every kilogram of starch
2+ion concentration is 210mg; After adding carbohydrase and Pullulanase, the vigor of corresponding every gram turmeric starch saccharification enzyme is 270U, and the vigor of Pullulanase is 25U.
4) probio protein feed preparation: after the Dioscorea. zingiberensis Wright Starch enzymolysis liquid is cooled to 48 ℃, add (NH
4)
2sO
4, peptone, anhydrous sodium acetate, diammonium hydrogen citrate, Tween 80, magnesium sulfate, manganese sulfate, dipotassium hydrogen phosphate, stir and regulate pH value to 6.0 and obtain mixed enzymolysis liquid, access again Paula enlightening yeast and lactobacillus acidophilus, 39 ℃ of bottom fermentation 55h, after will the yellow ginger fibrous residue adding, mix, and low temperature drying obtains yellow ginger probio albumen and raises.Concrete, add (the NH of 8.5g by every liter of Dioscorea. zingiberensis Wright Starch enzymolysis liquid
4)
2sO
4, the peptone of 4.0g, the anhydrous sodium acetate of 3.0g, the diammonium hydrogen citrate of 5.0g, the Tween 80 of 1.0mL, the magnesium sulfate of 0.2g, the manganese sulfate of 0.06g, the dipotassium hydrogen phosphate of 4.0g; Access respectively in proportion the enlightening yeast and lactobacillus acidophilus bacterial classification 8ml in the Paula of 4ml in every 100ml mixed enzymolysis liquid.
Example 5
A kind of method of utilizing yellow ginger to produce the probio protein feed, comprise the steps,
1) yellow ginger cellulosic and starch separate: after yellow ginger is cleaned to pulverizing, filtration obtains cellulosic; To leach that thing is standing is divided into three layers, upper strata is supernatant, and centre is suspension, and lower floor is starch, pours out supernatant standby, isolates lower floor's starch washing; Preferably, yellow ginger adopts the cadmium yellow ginger of water content 70%; Concrete employing 95 purpose filter-cloth filterings obtain cellulosic, are divided into three layers after leaching the standing 6h of thing.
2) yellow ginger cellulosic fermentation: add wheat bran, ammonium sulfate and Tween 80 in the yellow ginger cellulosic, and stir and obtain the cellulosic mixture, then sterilizing is cooled to 38 ℃ of access Trichoderma virides, after 31 ℃ of constant temperature culture 3d, obtains the yellow ginger fibrous residue of cellulase and mycoprotein; Concrete, add respectively in proportion wheat bran and the ammonium sulfate of 16g and the Tween 80 of 1.1ml of 24g in every kilogram of yellow ginger cellulosic in the yellow ginger cellulosic; Sterilizing 23min at the temperature of 121 ℃ after being cooled to 37 ℃, accesses the Trichoderma viride liquid spawn of 9ml in proportion in every 100mg cellulosic mixture.
3) Dioscorea. zingiberensis Wright Starch liquefying-saccharifying: the Dioscorea. zingiberensis Wright Starch after washing is added to supernatant, making solid content in starch milk is 12%, its pH value is adjusted to 6.2, and is warming up to 78 ℃, then add AMS and calcium chloride, and insulation enzymolysis 1.4h, then cool the temperature to 59 ℃, then the pH value is adjusted to 5.7, and add carbohydrase and Pullulanase, insulation enzymolysis 4h, obtain the Dioscorea. zingiberensis Wright Starch enzymolysis liquid; Concrete, after adding AMS and calcium chloride, the vigor of corresponding every gram starch AMS is 230U, the Ca in every kilogram of starch
2+ion concentration is 240mg; After adding carbohydrase and Pullulanase, the vigor of corresponding every gram turmeric starch saccharification enzyme is 290U, and the vigor of Pullulanase is 35U.
4) probio protein feed preparation: after the Dioscorea. zingiberensis Wright Starch enzymolysis liquid is cooled to 46 ℃, add (NH
4)
2sO
4, peptone, anhydrous sodium acetate, diammonium hydrogen citrate, Tween 80, magnesium sulfate, manganese sulfate, dipotassium hydrogen phosphate, stir and regulate pH value to 5.0 and obtain mixed enzymolysis liquid, access again Paula enlightening yeast and lactobacillus acidophilus, 35 ℃ of bottom fermentation 57h, after will the yellow ginger fibrous residue adding, mix, and low temperature drying obtains yellow ginger probio albumen and raises.Concrete, add (the NH of 9.5g by every liter of Dioscorea. zingiberensis Wright Starch enzymolysis liquid
4)
2sO
4, the peptone of 5.0g, the anhydrous sodium acetate of 2.0g, the diammonium hydrogen citrate of 6.0g, the Tween 80 of 0.8mL, the magnesium sulfate of 0.3g, the manganese sulfate of 0.04g, the dipotassium hydrogen phosphate of 2.0g; Access respectively in proportion the enlightening yeast and lactobacillus acidophilus bacterial classification 6ml in the Paula of 6ml in every 100ml mixed enzymolysis liquid.
Claims (10)
1. a method of utilizing yellow ginger to produce the probio protein feed, is characterized in that, comprise the steps,
1) yellow ginger cellulosic and starch separate: after yellow ginger is cleaned to pulverizing, filtration obtains cellulosic; To leach that thing is standing is divided into three layers, upper strata is supernatant, and centre is suspension, and lower floor is starch, pours out supernatant standby, isolates lower floor's starch washing;
2) yellow ginger cellulosic fermentation: add wheat bran, ammonium sulfate and Tween 80 in the yellow ginger cellulosic, and stir and obtain the cellulosic mixture, then sterilizing is cooled to 35-40 ℃ of access Trichoderma viride, after 27-33 ℃ of constant temperature culture 3-4d, obtain the yellow ginger fibrous residue of cellulase and mycoprotein;
3) Dioscorea. zingiberensis Wright Starch liquefying-saccharifying: the Dioscorea. zingiberensis Wright Starch after washing is added to supernatant, making solid content in starch milk is 8-12%, its pH value is adjusted to 6.2-6.5, and is warming up to 70-80 ℃, then add AMS and calcium chloride, and insulation enzymolysis 1-2h, then cool the temperature to 55-60 ℃, then the pH value is adjusted to 5.4-6.0, and add carbohydrase and Pullulanase, insulation enzymolysis 3-4h, obtain the Dioscorea. zingiberensis Wright Starch enzymolysis liquid;
4) probio protein feed preparation: after the Dioscorea. zingiberensis Wright Starch enzymolysis liquid is cooled to 45-50 ℃, add (NH
4)
2sO
4, peptone, anhydrous sodium acetate, diammonium hydrogen citrate, Tween 80, magnesium sulfate, manganese sulfate, dipotassium hydrogen phosphate, stir and regulate the pH value and obtain mixed enzymolysis liquid to 5.0-6.0, access again Paula enlightening yeast and lactobacillus acidophilus, 34-39 ℃ of bottom fermentation 48-60h, after the yellow ginger fibrous residue is added, mix, and low temperature drying obtains yellow ginger probio protein feed.
2. a kind of method of utilizing yellow ginger to produce the probio protein feed according to claim 1, is characterized in that, in step 1), yellow ginger adopts the cadmium yellow ginger of water content 65-70%.
3. a kind of method of utilizing yellow ginger to produce the probio protein feed according to claim 1, is characterized in that, in step 1), adopts 80-100 purpose filter-cloth filtering to obtain cellulosic, is divided into three layers after leaching the standing 5-6h of thing.
4. a kind of method of utilizing yellow ginger to produce the probio protein feed according to claim 1, it is characterized in that, step 2), in, add respectively in proportion wheat bran and the ammonium sulfate of 10-20g and the Tween 80 of 0.8-1.2ml of 20-25g in the yellow ginger cellulosic in every kilogram of yellow ginger cellulosic.
5. according to a kind of described method of utilizing yellow ginger to produce the probio protein feed of claim 1 or 4, it is characterized in that, step 2) in, sterilizing 20min-25min at the temperature of 121 ℃, after being cooled to 35 ℃-40 ℃, access in proportion the Trichoderma viride liquid spawn of 6-10ml in every 100mg cellulosic mixture.
6. a kind of method of utilizing yellow ginger to produce the probio protein feed according to claim 1, is characterized in that, in step 3), after adding AMS and calcium chloride, the vigor of corresponding every gram starch AMS is 200-240U, the Ca in every kilogram of starch
2+ion concentration is 200-240mg.
7. according to a kind of described method of utilizing yellow ginger to produce the probio protein feed of claim 1 or 6, it is characterized in that, in step 3), after adding carbohydrase and Pullulanase, the vigor of corresponding every gram turmeric starch saccharification enzyme is 240-300U, and the vigor of Pullulanase is 20-40U.
8. a kind of method of utilizing yellow ginger to produce the probio protein feed according to claim 1, is characterized in that, in step 4), adds (the NH of 8-10g by every liter of Dioscorea. zingiberensis Wright Starch enzymolysis liquid
4)
2sO
4, the peptone of 4-5g, the anhydrous sodium acetate of 2-3.0g, the diammonium hydrogen citrate of 5-6g, the Tween 80 of 0.8-1.0mL, the magnesium sulfate of 0.2-0.3g, the manganese sulfate of 0.04-0.06g, the dipotassium hydrogen phosphate of 2.0-4.0g.
9. according to a kind of described method of utilizing yellow ginger to produce the probio protein feed of claim 1 or 8, it is characterized in that, in step 4), access respectively in proportion the enlightening yeast and lactobacillus acidophilus bacterial classification 6-8ml in the Paula of 4-6ml in every 100ml mixed enzymolysis liquid.
10. a probio protein feed that utilizes yellow ginger to produce, is characterized in that, in claim 1-9, the described method of utilizing yellow ginger to produce the probio protein feed of any one makes.
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