CN103477991A - Primary culture medium specially used for tissue culture seedling culture of radix sophorae subprostratae - Google Patents
Primary culture medium specially used for tissue culture seedling culture of radix sophorae subprostratae Download PDFInfo
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- CN103477991A CN103477991A CN201310486772.6A CN201310486772A CN103477991A CN 103477991 A CN103477991 A CN 103477991A CN 201310486772 A CN201310486772 A CN 201310486772A CN 103477991 A CN103477991 A CN 103477991A
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Abstract
The invention provides a primary culture medium specially used for tissue culture seedling culture of radix sophorae subprostratae, for solving the problems of high difficulty and low seedling rate, existing in artificial breeding of radix sophorae subprostratae. The primary culture medium comprises an MS (Murashige and Skoog) culture medium, BA (Benzyl Adenine), NAA (Naphthalene Acetic Acid), sucrose and agar. By adoption of the primary culture medium disclosed by the invention, the inductivity of caespitose shoots is high up to 62%-83%, the successful tissue culture seedling culture of the radix sophorae subprostratae can be ensured and the demands of radix sophorae subprostratae seedlings in large-area artificial cultivation can be met.
Description
Technical field
The present invention relates to a kind of just culture base, be specifically related to a kind of first culture base of special use of subprostrate sophora tissue cultivating and seedling, belong to the tissue cultivating and seedling technical field.
Background technology
Subprostrate sophora, Latin is by name: Radix Sophorae Tonkinensis, also be root of subprostrate sophora, south beans root, for Dicotyledoneae leguminous plant sophora tonkinensis Gapnep, belong to natural crude drugs, medicinal part is root and rhizome, excavate autumn, have clearing heat and detoxicating, the function of swelling and pain relieving, be used for the treatment of abscess of throat, gingivitis, breathing heavily full heat coughs, hepatitis, constipation, jaundice, diarrhea, mange and mosquito, sting etc., phytomorph is shrub, high 1～2 meter, wild subprostrate sophora is born in Limestone Mountain or rock seam, main product Guangxi, also there is distribution in Yunnan and Guizhou, but output is less.
The medicinal part of subprostrate sophora is root system and rhizome, except the traditional Chinese medicine as traditional is used, medical industry is also usingd subprostrate sophora as raw material, a large amount of injections of developing treatment hepatitis, the tablet of abscess of throat and anti-swollen Chinese patent drug, therefore the demand of subprostrate sophora is increasing, but the Regional Distribution scope limited of subprostrate sophora, often sporadicly grow among tor rock seam, under field conditions (factors), the bloom damage by disease and insect of pod bearing period of subprostrate sophora is serious, during seed maturity, easily naturally come off, therefore the natural propagation coefficient is extremely low, and in order to meet the need of market, obtain economic interests, people excavate wild subprostrate sophora resource in a large number, cause wild subprostrate sophora resource endangered, for satisfying the demand, people have to develop artificial breeding technology, this solves the subprostrate sophora problem of growing seedlings with regard to needs, carrying out tissue cultivating and seedling is the most effective subprostrate sophora seedling-cultivating method without coagulating, and the tissue cultivating and seedling most critical is to prepare medium, medium comprises just culture base, subculture medium and root media, wherein just the effect of culture base is to make aseptic seedling induce Multiple Buds.
Summary of the invention
goal of the invention: the purpose of this invention is to provide the first culture base of a kind of subprostrate sophora group training seedling special use, improve the subprostrate sophora tissue and cultivate the inducing clumping bud rate, guarantee that the subprostrate sophora tissue cultivating and seedling obtains effect.
technical scheme: a kind of subprostrate sophora group training seedling of the present invention special use is the culture base just, just culture base is the MS medium that contains the naa (NAA) that benzyl purine (BA) that concentration is 1.5～2.4 mg/L and concentration are 0.1～0.3mg/L, that is to say and take the MS medium as basal medium, then the naa (NAA) that the benzyl purine (BA) that is 1.5～2.4 mg/L to MS basal medium interpolation concentration and concentration are 0.1～0.3mg/L, wherein the preferred concentration of benzyl purine (BA) is 2.0mg/L, the preferred concentration of naa (NAA) is 0.2mg/L, add 2% sucrose and 0.5% agar simultaneously, the medium temperature keeps 24 ℃～26 ℃, pH value 5.9～6.2, illumination 1600～1900Lx, 12 hours every days.
beneficial effect: compared with prior art, its beneficial effect is in the present invention:
Subprostrate sophora tissue of the present invention is cultivated special-purpose just culture base, can make the subprostrate sophora aseptic seedling form Multiple Buds, and the inducing clumping bud rate reaches 62%～83%, and draws materials conveniently.
embodiment
Below in conjunction with the concrete example of implementing, technical solution of the present invention is elaborated.
Embodiment: the first culture base that contains hormon is cultivated the experiment of subprostrate sophora aseptic seedling.
The subprostrate sophora seed is placed in to 0.2% mercuric chloride solution soaking disinfection 15 minutes, embathes 5 times with sterile water on superclean bench, embathe 3 minutes at every turn, then by planting seed in medium, under the Indoor Natural light scattering, cultivate, obtain the subprostrate sophora aseptic seedling.
After the subprostrate sophora aseptic seedling is grown to 5～6 centimetres, cut the top leaf stem section of top 2.6～2.9 centimeter length, 2. 1. access be numbered first culture base 3. respectively, cultivate after 28 days and have Multiple Buds to form, calculate different first culture bases, the inducing clumping bud rate is also different, and result is as following table.Visible cell mitogen BA and growth hormone NAA concentration are the factors that determines that Multiple Buds forms, the higher formation that may not be conducive to Multiple Buds of BA and NAA consumption.
Subordinate list: first culture base and the inducing clumping bud rate thereof of hormon concentration
Claims (3)
1. a subprostrate sophora group is trained just culture base of seedling special use, this special use just culture base comprises MS medium, benzyl purine (BA), naa (NAA), sucrose and agar, the concentration that it is characterized in that benzyl purine (BA) is 1.5～2.4 mg/L, the concentration of naa (NAA) is 0.1～0.3mg/L, the concentration of sucrose is 2%, and the concentration of agar is 0.5.
2. subprostrate sophora group according to claim 1 is trained just culture base of seedling special use, and the concentration that it is characterized in that described benzyl purine (BA) is 2.0 mg/L, and the concentration of naa (NAA) is 0.2mg/L.
3. the first culture base of subprostrate sophora group training seedling special use according to claim 1 and 2, is characterized in that 24 ℃～26 ℃ of just culture Ji Wendu maintenances of described special use, pH value 5.9～6.2.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201310486772.6A CN103477991A (en) | 2013-10-17 | 2013-10-17 | Primary culture medium specially used for tissue culture seedling culture of radix sophorae subprostratae |
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| CN201310486772.6A CN103477991A (en) | 2013-10-17 | 2013-10-17 | Primary culture medium specially used for tissue culture seedling culture of radix sophorae subprostratae |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104823854A (en) * | 2015-05-15 | 2015-08-12 | 黄振忠 | Primary culture medium special for panax notoginseng tissue culture seedlings |
| CN106508675A (en) * | 2016-10-27 | 2017-03-22 | 潘克稳 | Seed tissue culture medium for root of subprostrate sophora |
| CN106818490A (en) * | 2017-04-08 | 2017-06-13 | 广西科技大学鹿山学院 | A kind of culture medium for improving subprostrate sophora tissue-cultured seedling flavone compound and polysaccharide |
| CN108617504A (en) * | 2017-03-26 | 2018-10-09 | 黄振忠 | A kind of special Initial culture base of candidum tissue culturing seedling and preparation method thereof |
| CN112005884A (en) * | 2020-09-14 | 2020-12-01 | 广西壮族自治区药用植物园 | Method for improving alkaloid content of subprostrate sophora tissue culture seedling by utilizing selenium |
| CN112154914A (en) * | 2020-10-09 | 2021-01-01 | 广西壮族自治区药用植物园 | Method for improving alkaloid content of subprostrate sophora tissue culture seedling by using calcium ions |
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| CN101974478A (en) * | 2010-09-29 | 2011-02-16 | 成都大学 | Culture method for effectively improving total alkaloid content of Fritillaria cirrhosa D. Don |
| CN102715090A (en) * | 2012-07-11 | 2012-10-10 | 广西壮族自治区药用植物园 | Subprostrate sophora root tissue culture seedling industrialization production method |
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2013
- 2013-10-17 CN CN201310486772.6A patent/CN103477991A/en active Pending
Patent Citations (2)
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| CN101974478A (en) * | 2010-09-29 | 2011-02-16 | 成都大学 | Culture method for effectively improving total alkaloid content of Fritillaria cirrhosa D. Don |
| CN102715090A (en) * | 2012-07-11 | 2012-10-10 | 广西壮族自治区药用植物园 | Subprostrate sophora root tissue culture seedling industrialization production method |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104823854A (en) * | 2015-05-15 | 2015-08-12 | 黄振忠 | Primary culture medium special for panax notoginseng tissue culture seedlings |
| CN106508675A (en) * | 2016-10-27 | 2017-03-22 | 潘克稳 | Seed tissue culture medium for root of subprostrate sophora |
| CN108617504A (en) * | 2017-03-26 | 2018-10-09 | 黄振忠 | A kind of special Initial culture base of candidum tissue culturing seedling and preparation method thereof |
| CN106818490A (en) * | 2017-04-08 | 2017-06-13 | 广西科技大学鹿山学院 | A kind of culture medium for improving subprostrate sophora tissue-cultured seedling flavone compound and polysaccharide |
| CN112005884A (en) * | 2020-09-14 | 2020-12-01 | 广西壮族自治区药用植物园 | Method for improving alkaloid content of subprostrate sophora tissue culture seedling by utilizing selenium |
| CN112005884B (en) * | 2020-09-14 | 2022-02-22 | 广西壮族自治区药用植物园 | Method for improving alkaloid content of subprostrate sophora tissue culture seedling by utilizing selenium |
| CN112154914A (en) * | 2020-10-09 | 2021-01-01 | 广西壮族自治区药用植物园 | Method for improving alkaloid content of subprostrate sophora tissue culture seedling by using calcium ions |
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